RchiOBHm_Chr6g0262501

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0003526
Physical Location & Seq
Reverse (-)
17261151 .. 17263467
2317 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ23541

Sequence Viewer

Length: 480 bp
ATGAAGGTGAGGCTTGAGGCGACTAAGAAGAAGAGGAATTCTGTGCTCAAGTATTTGAAGAATGGTTTTGCCGATCGTGGCCTTGATATTAATGCATATGGAAGGAAAAAAATATCTGAAACTAAGGTGTTTCCGGATAAAGAAAAAATAAATAAGAGTTGCAAGGAAAGCTGTCCTGAAGAATGCAAGGAAGCTGTTGCATCTGTGATATATGCTACAACAAGATTTCTTGATTTACCAGAACTGCATGACCTCAGAAACATATTAGCTGAAAAATATGGAGATTCCGCACAATCTTTTGTAAATAAAGAGTTTGTGGACAAATTCAAGCCAAAGCCCCTTACAAAGGATATGAAGGTACAAGTACTAATGCATGACATAGCCAGAGAATACTCAATCGAATGGGGGATGGTGCCCTTGGGAGTTATAGTAGCAGTAGCAAGGCTAGTGTATGACTCAATTATAAAACTGAATGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

159

Amino Acids

18.2

Weight (kDa)

9.33

Isoelectric Point (pI)

38.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 55 - 134 3.8e-20 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 464
AccB1I GGYRCC 1 cut(s) 412
AccIII TCCGGA 1 cut(s) 133
AciI CCGC 1 cut(s) 288
AcsI RAATTY 2 cut(s) 37, 323
AcuI CTGAAG 1 cut(s) 198
AfaI GTAC 2 cut(s) 360, 366
AfiI CCNNNNNNNGG 1 cut(s) 346
AgsI TTSAA 2 cut(s) 58, 328
AluBI AGCT 3 cut(s) 171, 194, 269
AluI AGCT 3 cut(s) 171, 194, 269
Alw21I GWGCWC 1 cut(s) 48
Aor13HI TCCGGA 1 cut(s) 133
AoxI GGCC 1 cut(s) 79
ApoI RAATTY 2 cut(s) 37, 323
AseI ATTAAT 1 cut(s) 90
AsuHPI GGTGA 1 cut(s) 19
BaeGI GKGCMC 1 cut(s) 417
BanI GGYRCC 1 cut(s) 412
Bbv12I GWGCWC 1 cut(s) 48
BccI CCATC 1 cut(s) 403
BfaI CTAG 1 cut(s) 446
BmcAI AGTACT 1 cut(s) 366
BmiI GGNNCC 1 cut(s) 414
BmsI GCATC 1 cut(s) 209
BpuEI CTTGAG 2 cut(s) 32, 35
BsaJI CCNNGG 1 cut(s) 417
BsaWI WCCGGW 1 cut(s) 133
BsaXI ACNNNNNCTCC 2 cut(s) 414, 444
Bsc4I CCNNNNNNNGG 1 cut(s) 346
BseAI TCCGGA 1 cut(s) 133
BseDI CCNNGG 1 cut(s) 417
BseGI GGATG 1 cut(s) 414
BseLI CCNNNNNNNGG 1 cut(s) 346
BseMII CTCAG 1 cut(s) 268
BseSI GKGCMC 1 cut(s) 417
Bsh1285I CGRYCG 1 cut(s) 76
BshFI GGCC 1 cut(s) 81
BshNI GGYRCC 1 cut(s) 412
BsiEI CGRYCG 1 cut(s) 76
BsiHKAI GWGCWC 1 cut(s) 48
BsiSI CCGG 1 cut(s) 134
BslI CCNNNNNNNGG 1 cut(s) 346
BsmI GAATGC 1 cut(s) 188
BsnI GGCC 1 cut(s) 81
Bsp1286I GDGCHC 2 cut(s) 48, 417
Bsp13I TCCGGA 1 cut(s) 133
Bsp143I GATC 1 cut(s) 73
BspACI CCGC 1 cut(s) 288
BspANI GGCC 1 cut(s) 81
BspCNI CTCAG 1 cut(s) 267
BspEI TCCGGA 1 cut(s) 133
BspLI GGNNCC 1 cut(s) 414
BspT107I GGYRCC 1 cut(s) 412
BssECI CCNNGG 1 cut(s) 417
BssMI GATC 1 cut(s) 73
BssT1I CCWWGG 1 cut(s) 417
Bst6I CTCTTC 1 cut(s) 26
BstDEI CTNAG 3 cut(s) 24, 123, 254
BstENI CCTNNNNNAGG 1 cut(s) 344
BstF5I GGATG 1 cut(s) 414
BstKTI GATC 1 cut(s) 76
BstMBI GATC 1 cut(s) 73
BstMCI CGRYCG 1 cut(s) 76
BstMWI GCNNNNNNNGC 1 cut(s) 168
BstSLI GKGCMC 1 cut(s) 417
BsuRI GGCC 1 cut(s) 81
BtsCI GGATG 1 cut(s) 414
Csp6I GTAC 2 cut(s) 359, 365
CviAII CATG 2 cut(s) 248, 374
CviJI RGCY 9 cut(s) 13, 81, 171, 194, 269, 331, 337, 383, 445
CviKI_1 RGCY 9 cut(s) 13, 81, 171, 194, 269, 331, 337, 383, 445
CviQI GTAC 2 cut(s) 359, 365
DdeI CTNAG 3 cut(s) 24, 123, 254
DpnI GATC 1 cut(s) 75
DpnII GATC 1 cut(s) 73
Eam1104I CTCTTC 1 cut(s) 26
EarI CTCTTC 1 cut(s) 26
Eco130I CCWWGG 1 cut(s) 417
Eco57I CTGAAG 1 cut(s) 198
EcoNI CCTNNNNNAGG 1 cut(s) 344
EcoRI GAATTC 1 cut(s) 37
EcoT14I CCWWGG 1 cut(s) 417
EcoT22I ATGCAT 2 cut(s) 97, 375
ErhI CCWWGG 1 cut(s) 417
FaeI CATG 2 cut(s) 251, 377
FatI CATG 2 cut(s) 247, 373
FauNDI CATATG 1 cut(s) 97
FokI GGATG 1 cut(s) 421
FspBI CTAG 1 cut(s) 446
HaeIII GGCC 1 cut(s) 81
HapII CCGG 1 cut(s) 134
Hin1II CATG 2 cut(s) 251, 377
HinfI GANTC 2 cut(s) 284, 455
HpaII CCGG 1 cut(s) 134
HphI GGTGA 1 cut(s) 19
Hpy166II GTNNAC 1 cut(s) 319
Hpy188I TCNGA 3 cut(s) 118, 257, 479
Hpy188III TCNNGA 3 cut(s) 134, 176, 230
Hpy8I GTNNAC 1 cut(s) 319
HpyAV CCTTC 2 cut(s) 96, 349
HpyCH4V TGCA 6 cut(s) 95, 162, 186, 200, 247, 373
HpyF10VI GCNNNNNNNGC 1 cut(s) 168
HpyF3I CTNAG 3 cut(s) 24, 123, 254
Hsp92II CATG 2 cut(s) 251, 377
Kpn2I TCCGGA 1 cut(s) 133
Kzo9I GATC 1 cut(s) 73
LpnPI CCDG 4 cut(s) 147, 189, 252, 397
LweI GCATC 1 cut(s) 209
MaeI CTAG 1 cut(s) 446
MalI GATC 1 cut(s) 75
MboI GATC 1 cut(s) 73
MboII GAAGA 4 cut(s) 40, 43, 70, 191
MhlI GDGCHC 2 cut(s) 48, 417
MluCI AATT 3 cut(s) 37, 323, 459
MlyI GAGTC 1 cut(s) 449
MnlI CCTC 4 cut(s) 3, 10, 27, 263
Mph1103I ATGCAT 2 cut(s) 97, 375
MroI TCCGGA 1 cut(s) 133
MseI TTAA 1 cut(s) 90
MspI CCGG 1 cut(s) 134
Mva1269I GAATGC 1 cut(s) 188
MwoI GCNNNNNNNGC 1 cut(s) 168
NdeI CATATG 1 cut(s) 97
NdeII GATC 1 cut(s) 73
NlaIII CATG 2 cut(s) 251, 377
NlaIV GGNNCC 1 cut(s) 414
NsiI ATGCAT 2 cut(s) 97, 375
PctI GAATGC 1 cut(s) 188
PfeI GAWTC 1 cut(s) 284
Ple19I CGATCG 1 cut(s) 76
PleI GAGTC 1 cut(s) 449
PpsI GAGTC 1 cut(s) 449
PshBI ATTAAT 1 cut(s) 90
PsiI TTATAA 1 cut(s) 464
PspN4I GGNNCC 1 cut(s) 414
PvuI CGATCG 1 cut(s) 76
RsaI GTAC 2 cut(s) 360, 366
RsaNI GTAC 2 cut(s) 359, 365
SaqAI TTAA 1 cut(s) 90
Sau3AI GATC 1 cut(s) 73
ScaI AGTACT 1 cut(s) 366
SchI GAGTC 1 cut(s) 449
SduI GDGCHC 2 cut(s) 48, 417
SetI ASST 7 cut(s) 9, 129, 173, 196, 255, 271, 360
SfaNI GCATC 1 cut(s) 209
SmlI CTYRAG 2 cut(s) 14, 47
SmoI CTYRAG 2 cut(s) 14, 47
Sse9I AATT 3 cut(s) 37, 323, 459
SsiI CCGC 1 cut(s) 288
SspMI CTAG 1 cut(s) 446
StyI CCWWGG 1 cut(s) 417
TaqI TCGA 1 cut(s) 399
TasI AATT 3 cut(s) 37, 323, 459
TatI WGTACW 1 cut(s) 364
TfiI GAWTC 1 cut(s) 284
Tru1I TTAA 1 cut(s) 90
Tru9I TTAA 1 cut(s) 90
TspDTI ATGAA 2 cut(s) 17, 368
VspI ATTAAT 1 cut(s) 90
XagI CCTNNNNNAGG 1 cut(s) 344
XapI RAATTY 2 cut(s) 37, 323
XspI CTAG 1 cut(s) 446
ZrmI AGTACT 1 cut(s) 366
Zsp2I ATGCAT 2 cut(s) 97, 375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.