MD17G1194200.v1.1

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0003526
Physical Location & Seq
Forward (+)
23290243 .. 23293244
3002 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1194200.v1.1.491

Sequence Viewer

Length: 1422 bp
ATGTTGAGGGGGTTCCGAAAGCCGAGATTCGTCACCAAATGCAAATCGGATATAAAGATGACGAAGATGCGGCTCGAGGCGATAAAGAAGAAGAGGAACGCAGTGCAGAAGTTTTTGAAGAACGATATGGCTGATCTTTTGAGCAGTGGCCTTGGCATCAATGCTTATGGCAGGGCTGAGGGGCTTCTAGTTGAGCAAAATATGTCCGCCTGTTACGAATCAACAGAAAACTTTCTTGGATGCATCTCGAGTCATCTGTCTCTCATGCAAAGTCAGAGTGAGTGCCCTGAGGAATGCAAGGAAGCTGTTCCATCTTTGATGTACGCTGCAGCACGATTTTCTGATTTGCCAGAACTGCGTGACCTCCGAACTCTGTTTAGTGAAAAATATGGCAATTCTCTCGAACCTTTTCTGAATAAAGAGTTTGTGGAGAGGTTGAAATCAAAGCCTTCAACGAAGGAGATGAAGATTCAGTTAATGCATGACATAGCACGGGAATTCTCAATTGAATGGGATTCAAAGGCCTTGGAACAGAAACTGTATACTCCACCTGCACCAGACCAAGGTCAGCGCAGGTATGGGTCTCCAGTTGACAGCGATAACGATATGCACAAATCACATAAAGGGCAGGATAATGCTTTCCCGAAGAGAAGAAACCAGGATGCTGGGGACAAATTAAGCCACACAACGTCTTCTACTGCATCAGTAGGAAATGAGATCGACTTGAAACCTCAGGCAAGCAAGGGTGACTCTTATGACAGATACAAGCTGCAGAGCAGCAGTGAAGATGAAACCACAACAGACGCATCCCAATCTTCCCCAAAGTATTCTTCAAGCGCAGTAGGAAGCACATCCGCAGATGAAGCAGATAACAAGAAGATGCCCTTCCACTCCAAATTTATTCCACCACCTTACTACAAACCAAGACCTGGAAAAAAGGAAAGCAGTCACGGCGAAAAGGAAACAGATCAGCCTAGCAATGTTTCAGTCGACGAAGATAATACAAAACCAAGATCAGTAAGGTGGAAAAACTTGAAACCACCACCAGGTCGTGAGGATCTTGACAGGAACGATAAAACTAGACACGGAGATGATAGTGATCCAAGGGACAAAGAAGAGAGGATAATGGACGGGCTTTTGTTGCATTACAGCAAGAAACAGTCACCCTATGAATCGAGCCAGAAGAAGGGAAATGTAGACGAGTATGATGGTGGTGAATCCACATGGAAGAGAAGCAGAAGATCTCAGCGAGAAGCTCCATCGGCCAGAGCATCATCTCTGCCACCAGAGCCAACAAGTCCAATTGAGGCACCGAGAAAGCATGGCCGAGCAGTGTCCTTGGAACCGGATGCGCATGTGCACCCAAAGCTACCGGAGTATGATGAACTGGCAGCTCGAATTGCGGCTCTTAGGGGAATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

474

Amino Acids

53.32

Weight (kDa)

8.64

Isoelectric Point (pI)

65.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 15 - 169 1.3e-43 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 559
Acc16I TGCGCA 1 cut(s) 1353
Acc36I ACCTGC 2 cut(s) 559, 564
AccB1I GGYRCC 1 cut(s) 1309
AccB7I CCANNNNNTGG 1 cut(s) 929
AccI GTMKAC 3 cut(s) 542, 990, 1197
AciI CCGC 4 cut(s) 70, 207, 855, 1403
AclWI GGATC 2 cut(s) 1065, 1094
AcoI YGGCCR 2 cut(s) 1263, 1324
AcsI RAATTY 2 cut(s) 497, 896
AfaI GTAC 1 cut(s) 323
AfiI CCNNNNNNNGG 4 cut(s) 563, 929, 1046, 1186
AgsI TTSAA 8 cut(s) 118, 439, 453, 509, 519, 727, 834, 1036
AjnI CCWGG 3 cut(s) 657, 928, 1045
AjuI GAANNNNNNNTTGG 4 cut(s) 219, 251, 814, 846
AluBI AGCT 5 cut(s) 305, 769, 1256, 1369, 1394
AluI AGCT 5 cut(s) 305, 769, 1256, 1369, 1394
Alw21I GWGCWC 1 cut(s) 1362
Alw26I GTCTC 2 cut(s) 264, 588
Alw44I GTGCAC 1 cut(s) 1358
AlwI GGATC 2 cut(s) 1065, 1094
AlwNI CAGNNNCTG 1 cut(s) 538
Ama87I CYCGRG 2 cut(s) 74, 247
AoxI GGCC 4 cut(s) 148, 522, 1263, 1324
ApaLI GTGCAC 1 cut(s) 1358
ApeKI GCWGC 5 cut(s) 326, 329, 769, 777, 1391
ApoI RAATTY 2 cut(s) 497, 896
Asp700I GAANNNNTTC 3 cut(s) 231, 306, 408
AspLEI GCGC 3 cut(s) 573, 839, 1354
AsuHPI GGTGA 4 cut(s) 25, 758, 1155, 1226
AvaI CYCGRG 2 cut(s) 74, 247
AxyI CCTNAGG 2 cut(s) 288, 732
BaeGI GKGCMC 2 cut(s) 287, 1362
BanI GGYRCC 1 cut(s) 1309
BbsI GAAGAC 1 cut(s) 684
Bbv12I GWGCWC 1 cut(s) 1362
BbvCI CCTCAGC 1 cut(s) 177
BbvI GCAGC 5 cut(s) 313, 341, 756, 789, 1403
BccI CCATC 3 cut(s) 319, 1202, 1267
BceAI ACGGC 1 cut(s) 967
BcgI CGANNNNNNTGC 2 cut(s) 382, 416
BciT130I CCWGG 3 cut(s) 659, 930, 1047
BcoDI GTCTC 2 cut(s) 264, 588
BfaI CTAG 3 cut(s) 188, 975, 1080
BfmI CTRYAG 2 cut(s) 327, 770
BfuAI ACCTGC 2 cut(s) 559, 564
BglII AGATCT 1 cut(s) 1241
BisI GCNGC 7 cut(s) 71, 327, 330, 770, 778, 1392, 1404
BlsI GCNGC 7 cut(s) 72, 328, 331, 771, 779, 1393, 1405
Bme1390I CCNGG 3 cut(s) 659, 930, 1047
BmeT110I CYCGRG 2 cut(s) 74, 247
BmiI GGNNCC 3 cut(s) 14, 1311, 1344
BmrFI CCNGG 3 cut(s) 659, 930, 1047
BoxI GACNNNNGTC 1 cut(s) 564
BpiI GAAGAC 1 cut(s) 684
BpmI CTGGAG 1 cut(s) 570
Bpu10I CCTNAGC 1 cut(s) 177
BsaBI GATNNNNATC 1 cut(s) 1098
BsaI GGTCTC 1 cut(s) 588
BsaJI CCNNGG 5 cut(s) 151, 525, 562, 1103, 1338
BsaWI WCCGGW 2 cut(s) 1345, 1372
Bsc4I CCNNNNNNNGG 4 cut(s) 563, 929, 1046, 1186
Bse1I ACTGG 2 cut(s) 587, 1392
Bse21I CCTNAGG 2 cut(s) 288, 732
Bse3DI GCAATG 1 cut(s) 985
Bse8I GATNNNNATC 1 cut(s) 1098
BseBI CCWGG 3 cut(s) 659, 930, 1047
BseDI CCNNGG 5 cut(s) 151, 525, 562, 1103, 1338
BseGI GGATG 5 cut(s) 245, 667, 806, 851, 1354
BseJI GATNNNNATC 1 cut(s) 1098
BseLI CCNNNNNNNGG 4 cut(s) 563, 929, 1046, 1186
BseMI GCAATG 1 cut(s) 985
BseMII CTCAG 4 cut(s) 168, 279, 746, 1259
BseNI ACTGG 2 cut(s) 587, 1392
BseSI GKGCMC 2 cut(s) 287, 1362
BseXI GCAGC 5 cut(s) 313, 341, 756, 789, 1403
BseYI CCCAGC 1 cut(s) 665
BsgI GTGCAG 2 cut(s) 125, 537
BshFI GGCC 4 cut(s) 150, 524, 1265, 1326
BshNI GGYRCC 1 cut(s) 1309
BsiHKAI GWGCWC 1 cut(s) 1362
BsiHKCI CYCGRG 2 cut(s) 74, 247
BsiSI CCGG 2 cut(s) 1346, 1373
BslFI GGGAC 2 cut(s) 683, 1121
BslI CCNNNNNNNGG 4 cut(s) 563, 929, 1046, 1186
BsmAI GTCTC 2 cut(s) 264, 588
BsmFI GGGAC 2 cut(s) 683, 1121
BsmI GAATGC 1 cut(s) 299
BsnI GGCC 4 cut(s) 150, 524, 1265, 1326
Bso31I GGTCTC 1 cut(s) 588
BsoBI CYCGRG 2 cut(s) 74, 247
Bsp1286I GDGCHC 2 cut(s) 287, 1362
Bsp143I GATC 7 cut(s) 133, 717, 967, 1013, 1057, 1099, 1241
BspACI CCGC 4 cut(s) 70, 207, 855, 1403
BspANI GGCC 4 cut(s) 150, 524, 1265, 1326
BspCNI CTCAG 4 cut(s) 169, 280, 745, 1258
BspLI GGNNCC 3 cut(s) 14, 1311, 1344
BspMAI CTGCAG 2 cut(s) 331, 774
BspMI ACCTGC 2 cut(s) 559, 564
BspPI GGATC 2 cut(s) 1065, 1094
BspT107I GGYRCC 1 cut(s) 1309
BspTNI GGTCTC 1 cut(s) 588
BsrDI GCAATG 1 cut(s) 985
BsrI ACTGG 2 cut(s) 587, 1392
BssECI CCNNGG 5 cut(s) 151, 525, 562, 1103, 1338
BssMI GATC 7 cut(s) 133, 717, 967, 1013, 1057, 1099, 1241
BssNAI GTATAC 1 cut(s) 543
BssT1I CCWWGG 5 cut(s) 151, 525, 562, 1103, 1338
Bst1107I GTATAC 1 cut(s) 543
Bst2UI CCWGG 3 cut(s) 659, 930, 1047
Bst4CI ACNGT 2 cut(s) 540, 1161
Bst6I CTCTTC 4 cut(s) 86, 641, 1110, 1223
BstC8I GCNNGC 1 cut(s) 739
BstDEI CTNAG 5 cut(s) 177, 288, 732, 1245, 1409
BstF5I GGATG 5 cut(s) 245, 667, 806, 851, 1354
BstHHI GCGC 3 cut(s) 573, 839, 1354
BstKTI GATC 7 cut(s) 136, 720, 970, 1016, 1060, 1102, 1244
BstMAI GTCTC 2 cut(s) 264, 588
BstMBI GATC 7 cut(s) 133, 717, 967, 1013, 1057, 1099, 1241
BstMWI GCNNNNNNNGC 8 cut(s) 355, 863, 951, 1141, 1262, 1288, 1366, 1400
BstNI CCWGG 3 cut(s) 659, 930, 1047
BstNSI RCATGY 1 cut(s) 1358
BstPAI GACNNNNGTC 1 cut(s) 564
BstSCI CCNGG 3 cut(s) 657, 928, 1045
BstSFI CTRYAG 2 cut(s) 327, 770
BstSLI GKGCMC 2 cut(s) 287, 1362
BstV1I GCAGC 5 cut(s) 313, 341, 756, 789, 1403
BstV2I GAAGAC 1 cut(s) 684
BstX2I RGATCY 2 cut(s) 1057, 1241
BstXI CCANNNNNNTGG 1 cut(s) 665
BstYI RGATCY 2 cut(s) 1057, 1241
BstZ17I GTATAC 1 cut(s) 543
Bsu36I CCTNAGG 2 cut(s) 288, 732
BsuRI GGCC 4 cut(s) 150, 524, 1265, 1326
BtsCI GGATG 5 cut(s) 245, 667, 806, 851, 1354
BtsI GCAGTG 4 cut(s) 108, 151, 787, 1338
BtsIMutI CAGTG 4 cut(s) 108, 151, 787, 1338
BveI ACCTGC 2 cut(s) 559, 564
Cac8I GCNNGC 1 cut(s) 739
CaiI CAGNNNCTG 1 cut(s) 538
CfoI GCGC 3 cut(s) 573, 839, 1354
CseI GACGC 1 cut(s) 812
CsiI ACCWGGT 1 cut(s) 1045
Csp6I GTAC 1 cut(s) 322
CviAII CATG 5 cut(s) 265, 482, 1224, 1322, 1355
CviQI GTAC 1 cut(s) 322
DdeI CTNAG 5 cut(s) 177, 288, 732, 1245, 1409
DpnI GATC 7 cut(s) 135, 719, 969, 1015, 1059, 1101, 1243
DpnII GATC 7 cut(s) 133, 717, 967, 1013, 1057, 1099, 1241
EaeI YGGCCR 2 cut(s) 1263, 1324
Eam1104I CTCTTC 4 cut(s) 86, 641, 1110, 1223
EarI CTCTTC 4 cut(s) 86, 641, 1110, 1223
EciI GGCGGA 1 cut(s) 196
Eco130I CCWWGG 5 cut(s) 151, 525, 562, 1103, 1338
Eco147I AGGCCT 1 cut(s) 524
Eco31I GGTCTC 1 cut(s) 588
Eco81I CCTNAGG 2 cut(s) 288, 732
Eco88I CYCGRG 2 cut(s) 74, 247
EcoRI GAATTC 1 cut(s) 497
EcoRII CCWGG 3 cut(s) 657, 928, 1045
EcoT14I CCWWGG 5 cut(s) 151, 525, 562, 1103, 1338
EcoT22I ATGCAT 2 cut(s) 245, 483
ErhI CCWWGG 5 cut(s) 151, 525, 562, 1103, 1338
FaeI CATG 5 cut(s) 268, 485, 1227, 1325, 1358
FalI AAGNNNNNCTT 2 cut(s) 869, 901
FaqI GGGAC 2 cut(s) 683, 1121
FatI CATG 5 cut(s) 264, 481, 1223, 1321, 1354
FblI GTMKAC 3 cut(s) 542, 990, 1197
Fnu4HI GCNGC 7 cut(s) 71, 327, 330, 770, 778, 1392, 1404
FokI GGATG 5 cut(s) 252, 674, 793, 838, 1361
Fsp4HI GCNGC 7 cut(s) 71, 327, 330, 770, 778, 1392, 1404
FspAI RTGCGCAY 1 cut(s) 1353
FspBI CTAG 3 cut(s) 188, 975, 1080
FspI TGCGCA 1 cut(s) 1353
GlaI GCGC 3 cut(s) 572, 838, 1353
GluI GCNGC 7 cut(s) 71, 327, 330, 770, 778, 1392, 1404
GsaI CCCAGC 1 cut(s) 669
GsuI CTGGAG 1 cut(s) 570
HaeIII GGCC 4 cut(s) 150, 524, 1265, 1326
HapII CCGG 2 cut(s) 1346, 1373
HgaI GACGC 1 cut(s) 812
HhaI GCGC 3 cut(s) 573, 839, 1354
Hin1II CATG 5 cut(s) 268, 485, 1227, 1325, 1358
Hin6I GCGC 3 cut(s) 571, 837, 1352
HinP1I GCGC 3 cut(s) 571, 837, 1352
HincII GTYRAC 2 cut(s) 592, 991
HindII GTYRAC 2 cut(s) 592, 991
HinfI GANTC 8 cut(s) 27, 218, 250, 469, 515, 749, 1172, 1217
HpaII CCGG 2 cut(s) 1346, 1373
HphI GGTGA 4 cut(s) 25, 758, 1155, 1226
Hpy166II GTNNAC 5 cut(s) 543, 592, 991, 1198, 1360
Hpy188I TCNGA 6 cut(s) 17, 49, 276, 343, 368, 414
Hpy188III TCNNGA 5 cut(s) 247, 401, 643, 1052, 1061
Hpy8I GTNNAC 5 cut(s) 543, 592, 991, 1198, 1360
Hpy99I CGWCG 1 cut(s) 995
HpyAV CCTTC 4 cut(s) 451, 459, 895, 1180
HpyCH4III ACNGT 2 cut(s) 540, 1161
HpyCH4IV ACGT 1 cut(s) 689
HpyF10VI GCNNNNNNNGC 8 cut(s) 355, 863, 951, 1141, 1262, 1288, 1366, 1400
HpyF3I CTNAG 5 cut(s) 177, 288, 732, 1245, 1409
HpySE526I ACGT 1 cut(s) 689
Hsp92II CATG 5 cut(s) 268, 485, 1227, 1325, 1358
HspAI GCGC 3 cut(s) 571, 837, 1352
Kzo9I GATC 7 cut(s) 133, 717, 967, 1013, 1057, 1099, 1241
LmnI GCTCC 1 cut(s) 1261
Lsp1109I GCAGC 5 cut(s) 313, 341, 756, 789, 1403
MabI ACCWGGT 1 cut(s) 1045
MaeI CTAG 3 cut(s) 188, 975, 1080
MaeII ACGT 1 cut(s) 689
MaeIII GTNAC 6 cut(s) 31, 212, 359, 746, 947, 1161
MalI GATC 7 cut(s) 135, 719, 969, 1015, 1059, 1101, 1243
MboI GATC 7 cut(s) 133, 717, 967, 1013, 1057, 1099, 1241
MfeI CAATTG 2 cut(s) 504, 1302
MflI RGATCY 2 cut(s) 1057, 1241
MhlI GDGCHC 2 cut(s) 287, 1362
MluCI AATT 7 cut(s) 394, 497, 504, 674, 896, 1302, 1398
MlyI GAGTC 2 cut(s) 259, 743
Mph1103I ATGCAT 2 cut(s) 245, 483
MroXI GAANNNNTTC 3 cut(s) 231, 306, 408
MseI TTAA 2 cut(s) 476, 677
MslI CAYNNNNRTG 1 cut(s) 1089
MspI CCGG 2 cut(s) 1346, 1373
MspR9I CCNGG 3 cut(s) 659, 930, 1047
MunI CAATTG 2 cut(s) 504, 1302
Mva1269I GAATGC 1 cut(s) 299
MvaI CCWGG 3 cut(s) 659, 930, 1047
MwoI GCNNNNNNNGC 8 cut(s) 355, 863, 951, 1141, 1262, 1288, 1366, 1400
NdeII GATC 7 cut(s) 133, 717, 967, 1013, 1057, 1099, 1241
NlaIII CATG 5 cut(s) 268, 485, 1227, 1325, 1358
NlaIV GGNNCC 3 cut(s) 14, 1311, 1344
NmeAIII GCCGAG 2 cut(s) 48, 1352
NmuCI GTSAC 5 cut(s) 31, 359, 746, 947, 1161
NsbI TGCGCA 1 cut(s) 1353
NsiI ATGCAT 2 cut(s) 245, 483
NspI RCATGY 1 cut(s) 1358
PaeR7I CTCGAG 2 cut(s) 74, 247
PaqCI CACCTGC 1 cut(s) 559
PceI AGGCCT 1 cut(s) 524
PctI GAATGC 1 cut(s) 299
PdmI GAANNNNTTC 3 cut(s) 231, 306, 408
PfeI GAWTC 6 cut(s) 27, 218, 469, 515, 1172, 1217
PflMI CCANNNNNTGG 1 cut(s) 929
PkrI GCNGC 7 cut(s) 72, 328, 331, 771, 779, 1393, 1405
PleI GAGTC 2 cut(s) 258, 743
PpsI GAGTC 2 cut(s) 258, 743
PshAI GACNNNNGTC 1 cut(s) 564
Psp6I CCWGG 3 cut(s) 657, 928, 1045
PspFI CCCAGC 1 cut(s) 665
PspGI CCWGG 3 cut(s) 657, 928, 1045
PspN4I GGNNCC 3 cut(s) 14, 1311, 1344
PspXI VCTCGAGB 1 cut(s) 74
PstI CTGCAG 2 cut(s) 331, 774
PstNI CAGNNNCTG 1 cut(s) 538
PsuI RGATCY 2 cut(s) 1057, 1241
RsaI GTAC 1 cut(s) 323
RsaNI GTAC 1 cut(s) 322
RseI CAYNNNNRTG 1 cut(s) 1089
SalI GTCGAC 1 cut(s) 989
SaqAI TTAA 2 cut(s) 476, 677
SatI GCNGC 7 cut(s) 71, 327, 330, 770, 778, 1392, 1404
Sau3AI GATC 7 cut(s) 133, 717, 967, 1013, 1057, 1099, 1241
SchI GAGTC 2 cut(s) 259, 743
ScrFI CCNGG 3 cut(s) 659, 930, 1047
SduI GDGCHC 2 cut(s) 287, 1362
SexAI ACCWGGT 1 cut(s) 1045
SfcI CTRYAG 2 cut(s) 327, 770
Sfr274I CTCGAG 2 cut(s) 74, 247
SlaI CTCGAG 2 cut(s) 74, 247
SmiMI CAYNNNNRTG 1 cut(s) 1089
SmlI CTYRAG 2 cut(s) 74, 247
SmoI CTYRAG 2 cut(s) 74, 247
Sse9I AATT 7 cut(s) 394, 497, 504, 674, 896, 1302, 1398
SseBI AGGCCT 1 cut(s) 524
SsiI CCGC 4 cut(s) 70, 207, 855, 1403
SspMI CTAG 3 cut(s) 188, 975, 1080
StuI AGGCCT 1 cut(s) 524
StyD4I CCNGG 3 cut(s) 657, 928, 1045
StyI CCWWGG 5 cut(s) 151, 525, 562, 1103, 1338
TaaI ACNGT 2 cut(s) 540, 1161
TaiI ACGT 1 cut(s) 692
TaqI TCGA 7 cut(s) 75, 248, 402, 720, 990, 1175, 1396
TasI AATT 7 cut(s) 394, 497, 504, 674, 896, 1302, 1398
TauI GCSGC 2 cut(s) 73, 1406
TfiI GAWTC 6 cut(s) 27, 218, 469, 515, 1172, 1217
Tru1I TTAA 2 cut(s) 476, 677
Tru9I TTAA 2 cut(s) 476, 677
TscAI CASTG 4 cut(s) 108, 151, 787, 1338
TseFI GTSAC 5 cut(s) 31, 359, 746, 947, 1161
TseI GCWGC 5 cut(s) 326, 329, 769, 777, 1391
Tsp45I GTSAC 5 cut(s) 31, 359, 746, 947, 1161
TspDTI ATGAA 5 cut(s) 479, 804, 876, 1185, 1398
TspGWI ACGGA 1 cut(s) 1101
TspRI CASTG 4 cut(s) 108, 151, 787, 1338
Van91I CCANNNNNTGG 1 cut(s) 929
VneI GTGCAC 1 cut(s) 1358
XapI RAATTY 2 cut(s) 497, 896
XceI RCATGY 1 cut(s) 1358
XhoI CTCGAG 2 cut(s) 74, 247
XmiI GTMKAC 3 cut(s) 542, 990, 1197
XmnI GAANNNNTTC 3 cut(s) 231, 306, 408
XspI CTAG 3 cut(s) 188, 975, 1080
Zsp2I ATGCAT 2 cut(s) 245, 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.