Rroxscaffold_2G00106570

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0003526
Physical Location & Seq
Forward (+)
30155571 .. 30158997
3427 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00106570.1

Sequence Viewer

Length: 1287 bp
ATGTTTGGGTTCGGGAGGCCTAAATTTGTCACTAAATGCAAATCTGATTTAAAGGGCATGAAGGTGAGGCTTGAGGCGATTAAGAAGAAGAGGAACTCTGTGCTTAAGTATTTGAAGAATGATGTTGCCGAGCTTCTCAAGAGTGGCCTTGATATTAATGCATATGGAAGGGCTGAGGGAGTTCTGGTTGAGCAAAATATGTCCGCCTGTTATGCACTGACTGAGAACTTTCTTGACTGTATCGCAAATCATCTTTCTCTCATGCAAAATCAGAGGGAATGTCCTGAAGAATGCAAGGAAGCTGTTGCATCTGTGATATATGCAGCAGCAAGATTTTCTGATTTGCCAGAACTGCGTGACCTCAGAAACATATTTACTCAAAAGTATGGAGATTCCCTACAATCTTTTGTAAATAAAGAGTTTGCGGATAAATTCAATTCAAAGCCCCCTACAAAGGAGATGAAGGTACAATTAATGCATGACATAGCAAGAGAATACTCAATCGAATGGGACTCCAAGGCTCTGGAACAAAAGCTGTTTACTCCACCTCCAACAAACAAGGGGCAGCCTAGGTATGGGTCTCCATGTGACAGTGATGATGATATGTACAGATCTGAGAAGAAGGATAATGCTCTTCCGAAGAGAAAAAATAAGGGTGATGGAGAAAAACGGAGCTATGTAACTGATGATACTGCATCAGTAGGTAGTGATGATGAAGCAACAGATATGTCCCATGATGGCCGAATGACTGCTTCAAGTTCAGTAGGAAGTAGATCTGAGGATGAAGCAGAAAACAAGGTGCCCTCTTCCTATAAGTTCACTGCACCCCCTTACTACAAACCAAAGCCTCAGAAAAAAGAAAGTGTCTTTGAGGAACCCAGGAAAGTGAATGGTCATGCTGAACGAAGATCAGTTAGGAGAACCTCGAAACCACCAGCAGGTGGCGACAATCATGGAAGTGATCCGAGGGATGAAGAAGAGAGGGTAATGGATGGGCTTTTGATGCATTACAGTCATAAACAGTCACCCTATGAGTCAAGCAAGATGAAGGGAAATCTAAAACCTTGCTACAACCAGTCAGATGAAGATACTAATGAATCCACAAGGCACAGAAGCAGCAGGTCTGAAAGAGCAGCATCTCTACCACCACCAGAGCAATCTACTTCACCTGAGCCAACAAAAAGGCATGCTCGAGGCAGTTCCTTACAACCAGATGCCCATGTGCACCCGAACCTACCAGAGTATGATGAATTGGCAGCCCGAATTGCAGCTCTTAGAGGAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

428

Amino Acids

48.24

Weight (kDa)

8.27

Isoelectric Point (pI)

64.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 14 - 169 1.3e-46 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 929
Acc36I ACCTGC 2 cut(s) 929, 1110
AccB1I GGYRCC 1 cut(s) 799
AccB7I CCANNNNNTGG 1 cut(s) 941
AciI CCGC 2 cut(s) 204, 425
AclWI GGATC 1 cut(s) 956
AcoI YGGCCR 1 cut(s) 739
AcsI RAATTY 2 cut(s) 23, 431
AcuI CTGAAG 1 cut(s) 306
AfaI GTAC 2 cut(s) 468, 608
AfiI CCNNNNNNNGG 4 cut(s) 454, 575, 938, 941
AflII CTTAAG 1 cut(s) 104
AgsI TTSAA 4 cut(s) 115, 436, 441, 756
AjnI CCWGG 1 cut(s) 878
AluBI AGCT 5 cut(s) 133, 302, 535, 675, 1271
AluI AGCT 5 cut(s) 133, 302, 535, 675, 1271
Alw21I GWGCWC 1 cut(s) 1227
Alw26I GTCTC 1 cut(s) 585
Alw44I GTGCAC 1 cut(s) 1223
AlwI GGATC 1 cut(s) 956
Ama87I CYCGRG 1 cut(s) 1191
AoxI GGCC 3 cut(s) 17, 145, 739
ApaLI GTGCAC 1 cut(s) 1223
ApeKI GCWGC 7 cut(s) 323, 326, 565, 1116, 1133, 1256, 1268
ApoI RAATTY 2 cut(s) 23, 431
AseI ATTAAT 2 cut(s) 156, 473
AspA2I CCTAGG 1 cut(s) 569
AsuHPI GGTGA 4 cut(s) 76, 668, 1017, 1158
AvaI CYCGRG 1 cut(s) 1191
AvrII CCTAGG 1 cut(s) 569
BaeGI GKGCMC 2 cut(s) 804, 1227
BanI GGYRCC 1 cut(s) 799
Bbv12I GWGCWC 1 cut(s) 1227
BbvCI CCTCAGC 1 cut(s) 174
BbvI GCAGC 7 cut(s) 335, 338, 577, 1128, 1145, 1268, 1280
BccI CCATC 3 cut(s) 653, 731, 986
BciT130I CCWGG 1 cut(s) 880
BcoDI GTCTC 1 cut(s) 585
BfaI CTAG 1 cut(s) 570
BfrI CTTAAG 1 cut(s) 104
BfuAI ACCTGC 2 cut(s) 929, 1110
BglII AGATCT 2 cut(s) 611, 773
BisI GCNGC 7 cut(s) 324, 327, 566, 1117, 1134, 1257, 1269
BlnI CCTAGG 1 cut(s) 569
BlsI GCNGC 7 cut(s) 325, 328, 567, 1118, 1135, 1258, 1270
Bme1390I CCNGG 1 cut(s) 880
BmeT110I CYCGRG 1 cut(s) 1191
BmiI GGNNCC 2 cut(s) 801, 876
BmrFI CCNGG 1 cut(s) 880
BmsI GCATC 5 cut(s) 317, 704, 993, 1145, 1204
Bpu10I CCTNAGC 2 cut(s) 174, 1170
BpuEI CTTGAG 2 cut(s) 92, 122
BsaI GGTCTC 1 cut(s) 585
BsaJI CCNNGG 4 cut(s) 516, 569, 878, 965
BsaXI ACNNNNNCTCC 4 cut(s) 171, 201, 532, 562
Bsc4I CCNNNNNNNGG 4 cut(s) 454, 575, 938, 941
Bse1I ACTGG 1 cut(s) 1075
BseBI CCWGG 1 cut(s) 880
BseDI CCNNGG 4 cut(s) 516, 569, 878, 965
BseGI GGATG 3 cut(s) 787, 976, 997
BseLI CCNNNNNNNGG 4 cut(s) 454, 575, 938, 941
BseMII CTCAG 7 cut(s) 165, 213, 376, 606, 768, 863, 1161
BseNI ACTGG 1 cut(s) 1075
BseSI GKGCMC 2 cut(s) 804, 1227
BseXI GCAGC 7 cut(s) 335, 338, 577, 1128, 1145, 1268, 1280
BsgI GTGCAG 1 cut(s) 807
BshFI GGCC 3 cut(s) 19, 147, 741
BshNI GGYRCC 1 cut(s) 799
BsiHKAI GWGCWC 1 cut(s) 1227
BsiHKCI CYCGRG 1 cut(s) 1191
BslFI GGGAC 2 cut(s) 524, 715
BslI CCNNNNNNNGG 4 cut(s) 454, 575, 938, 941
BsmAI GTCTC 1 cut(s) 585
BsmFI GGGAC 2 cut(s) 524, 715
BsmI GAATGC 1 cut(s) 296
BsnI GGCC 3 cut(s) 19, 147, 741
Bso31I GGTCTC 1 cut(s) 585
BsoBI CYCGRG 1 cut(s) 1191
Bsp1286I GDGCHC 2 cut(s) 804, 1227
Bsp1407I TGTACA 1 cut(s) 606
Bsp143I GATC 4 cut(s) 611, 773, 908, 961
BspACI CCGC 2 cut(s) 204, 425
BspANI GGCC 3 cut(s) 19, 147, 741
BspCNI CTCAG 7 cut(s) 166, 214, 375, 607, 769, 862, 1162
BspLI GGNNCC 2 cut(s) 801, 876
BspMI ACCTGC 2 cut(s) 929, 1110
BspPI GGATC 1 cut(s) 956
BspQI GCTCTTC 1 cut(s) 639
BspT107I GGYRCC 1 cut(s) 799
BspTI CTTAAG 1 cut(s) 104
BspTNI GGTCTC 1 cut(s) 585
BsrGI TGTACA 1 cut(s) 606
BsrI ACTGG 1 cut(s) 1075
BssECI CCNNGG 4 cut(s) 516, 569, 878, 965
BssMI GATC 4 cut(s) 611, 773, 908, 961
BssT1I CCWWGG 2 cut(s) 516, 569
Bst2UI CCWGG 1 cut(s) 880
Bst4CI ACNGT 4 cut(s) 239, 593, 1013, 1023
Bst6I CTCTTC 5 cut(s) 83, 635, 639, 811, 972
BstAFI CTTAAG 1 cut(s) 104
BstAUI TGTACA 1 cut(s) 606
BstC8I GCNNGC 1 cut(s) 1188
BstDEI CTNAG 8 cut(s) 174, 222, 362, 615, 777, 849, 1170, 1274
BstF5I GGATG 3 cut(s) 787, 976, 997
BstKTI GATC 4 cut(s) 614, 776, 911, 964
BstMAI GTCTC 1 cut(s) 585
BstMBI GATC 4 cut(s) 611, 773, 908, 961
BstMWI GCNNNNNNNGC 4 cut(s) 212, 352, 1003, 1265
BstNI CCWGG 1 cut(s) 880
BstNSI RCATGY 1 cut(s) 1190
BstSCI CCNGG 1 cut(s) 878
BstSLI GKGCMC 2 cut(s) 804, 1227
BstV1I GCAGC 7 cut(s) 335, 338, 577, 1128, 1145, 1268, 1280
BstX2I RGATCY 2 cut(s) 611, 773
BstXI CCANNNNNNTGG 1 cut(s) 523
BstYI RGATCY 2 cut(s) 611, 773
BsuRI GGCC 3 cut(s) 19, 147, 741
BtsCI GGATG 3 cut(s) 787, 976, 997
BtsI GCAGTG 1 cut(s) 819
BtsIMutI CAGTG 3 cut(s) 215, 598, 819
BveI ACCTGC 2 cut(s) 929, 1110
Cac8I GCNNGC 1 cut(s) 1188
Csp6I GTAC 2 cut(s) 467, 607
CviQI GTAC 2 cut(s) 467, 607
DdeI CTNAG 8 cut(s) 174, 222, 362, 615, 777, 849, 1170, 1274
DpnI GATC 4 cut(s) 613, 775, 910, 963
DpnII GATC 4 cut(s) 611, 773, 908, 961
DraI TTTAAA 1 cut(s) 51
EaeI YGGCCR 1 cut(s) 739
Eam1104I CTCTTC 5 cut(s) 83, 635, 639, 811, 972
EarI CTCTTC 5 cut(s) 83, 635, 639, 811, 972
EciI GGCGGA 1 cut(s) 193
Eco130I CCWWGG 2 cut(s) 516, 569
Eco147I AGGCCT 1 cut(s) 19
Eco31I GGTCTC 1 cut(s) 585
Eco57I CTGAAG 1 cut(s) 306
Eco88I CYCGRG 1 cut(s) 1191
EcoRII CCWGG 1 cut(s) 878
EcoT14I CCWWGG 2 cut(s) 516, 569
EcoT22I ATGCAT 3 cut(s) 163, 480, 1008
ErhI CCWWGG 2 cut(s) 516, 569
FaqI GGGAC 2 cut(s) 524, 715
FauNDI CATATG 1 cut(s) 163
Fnu4HI GCNGC 7 cut(s) 324, 327, 566, 1117, 1134, 1257, 1269
FokI GGATG 3 cut(s) 794, 983, 1004
Fsp4HI GCNGC 7 cut(s) 324, 327, 566, 1117, 1134, 1257, 1269
FspBI CTAG 1 cut(s) 570
GluI GCNGC 7 cut(s) 324, 327, 566, 1117, 1134, 1257, 1269
HaeIII GGCC 3 cut(s) 19, 147, 741
HinfI GANTC 4 cut(s) 392, 512, 1034, 1097
HphI GGTGA 4 cut(s) 76, 668, 1017, 1158
Hpy166II GTNNAC 3 cut(s) 540, 819, 1225
Hpy188III TCNNGA 5 cut(s) 13, 139, 233, 284, 524
Hpy8I GTNNAC 3 cut(s) 540, 819, 1225
HpyAV CCTTC 5 cut(s) 55, 162, 457, 616, 1042
HpyCH4III ACNGT 4 cut(s) 239, 593, 1013, 1023
HpyF10VI GCNNNNNNNGC 4 cut(s) 212, 352, 1003, 1265
HpyF3I CTNAG 8 cut(s) 174, 222, 362, 615, 777, 849, 1170, 1274
Kzo9I GATC 4 cut(s) 611, 773, 908, 961
LguI GCTCTTC 1 cut(s) 639
LmnI GCTCC 1 cut(s) 672
Lsp1109I GCAGC 7 cut(s) 335, 338, 577, 1128, 1145, 1268, 1280
LweI GCATC 5 cut(s) 317, 704, 993, 1145, 1204
MaeI CTAG 1 cut(s) 570
MaeIII GTNAC 5 cut(s) 28, 356, 587, 679, 1023
MalI GATC 4 cut(s) 613, 775, 910, 963
MboI GATC 4 cut(s) 611, 773, 908, 961
MflI RGATCY 2 cut(s) 611, 773
MhlI GDGCHC 2 cut(s) 804, 1227
MluCI AATT 6 cut(s) 23, 431, 436, 470, 1250, 1263
MlyI GAGTC 2 cut(s) 506, 1043
MmeI TCCRAC 1 cut(s) 575
Mph1103I ATGCAT 3 cut(s) 163, 480, 1008
MseI TTAA 5 cut(s) 50, 81, 105, 156, 473
MslI CAYNNNNRTG 2 cut(s) 62, 957
MspCI CTTAAG 1 cut(s) 104
MspR9I CCNGG 1 cut(s) 880
Mva1269I GAATGC 1 cut(s) 296
MvaI CCWGG 1 cut(s) 880
MwoI GCNNNNNNNGC 4 cut(s) 212, 352, 1003, 1265
NdeI CATATG 1 cut(s) 163
NdeII GATC 4 cut(s) 611, 773, 908, 961
NlaIV GGNNCC 2 cut(s) 801, 876
NmeAIII GCCGAG 1 cut(s) 154
NmuCI GTSAC 4 cut(s) 28, 356, 587, 1023
NsiI ATGCAT 3 cut(s) 163, 480, 1008
NspI RCATGY 1 cut(s) 1190
PaeI GCATGC 1 cut(s) 1190
PaeR7I CTCGAG 1 cut(s) 1191
PaqCI CACCTGC 1 cut(s) 929
PceI AGGCCT 1 cut(s) 19
PciSI GCTCTTC 1 cut(s) 639
PctI GAATGC 1 cut(s) 296
PfeI GAWTC 2 cut(s) 392, 1097
PflMI CCANNNNNTGG 1 cut(s) 941
PkrI GCNGC 7 cut(s) 325, 328, 567, 1118, 1135, 1258, 1270
PleI GAGTC 2 cut(s) 506, 1042
PpsI GAGTC 2 cut(s) 506, 1042
PshBI ATTAAT 2 cut(s) 156, 473
Psp6I CCWGG 1 cut(s) 878
PspGI CCWGG 1 cut(s) 878
PspN4I GGNNCC 2 cut(s) 801, 876
PspXI VCTCGAGB 1 cut(s) 1191
PsuI RGATCY 2 cut(s) 611, 773
RsaI GTAC 2 cut(s) 468, 608
RsaNI GTAC 2 cut(s) 467, 607
RseI CAYNNNNRTG 2 cut(s) 62, 957
SapI GCTCTTC 1 cut(s) 639
SaqAI TTAA 5 cut(s) 50, 81, 105, 156, 473
SatI GCNGC 7 cut(s) 324, 327, 566, 1117, 1134, 1257, 1269
Sau3AI GATC 4 cut(s) 611, 773, 908, 961
SchI GAGTC 2 cut(s) 506, 1043
ScrFI CCNGG 1 cut(s) 880
SduI GDGCHC 2 cut(s) 804, 1227
SfaNI GCATC 5 cut(s) 317, 704, 993, 1145, 1204
Sfr274I CTCGAG 1 cut(s) 1191
SlaI CTCGAG 1 cut(s) 1191
SmiMI CAYNNNNRTG 2 cut(s) 62, 957
SmlI CTYRAG 4 cut(s) 71, 104, 137, 1191
SmoI CTYRAG 4 cut(s) 71, 104, 137, 1191
SphI GCATGC 1 cut(s) 1190
Sse9I AATT 6 cut(s) 23, 431, 436, 470, 1250, 1263
SseBI AGGCCT 1 cut(s) 19
SsiI CCGC 2 cut(s) 204, 425
SspMI CTAG 1 cut(s) 570
StuI AGGCCT 1 cut(s) 19
StyD4I CCNGG 1 cut(s) 878
StyI CCWWGG 2 cut(s) 516, 569
TaaI ACNGT 4 cut(s) 239, 593, 1013, 1023
TaqI TCGA 3 cut(s) 504, 926, 1192
TasI AATT 6 cut(s) 23, 431, 436, 470, 1250, 1263
TatI WGTACW 1 cut(s) 606
TfiI GAWTC 2 cut(s) 392, 1097
Tru1I TTAA 5 cut(s) 50, 81, 105, 156, 473
Tru9I TTAA 5 cut(s) 50, 81, 105, 156, 473
TscAI CASTG 3 cut(s) 222, 598, 826
TseFI GTSAC 4 cut(s) 28, 356, 587, 1023
TseI GCWGC 7 cut(s) 323, 326, 565, 1116, 1133, 1256, 1268
Tsp45I GTSAC 4 cut(s) 28, 356, 587, 1023
TspDTI ATGAA 9 cut(s) 74, 476, 729, 798, 987, 1061, 1098, 1110, 1263
TspGWI ACGGA 1 cut(s) 685
TspRI CASTG 3 cut(s) 222, 598, 826
Van91I CCANNNNNTGG 1 cut(s) 941
Vha464I CTTAAG 1 cut(s) 104
VneI GTGCAC 1 cut(s) 1223
VspI ATTAAT 2 cut(s) 156, 473
XapI RAATTY 2 cut(s) 23, 431
XceI RCATGY 1 cut(s) 1190
XhoI CTCGAG 1 cut(s) 1191
XmaJI CCTAGG 1 cut(s) 569
XspI CTAG 1 cut(s) 570
Zsp2I ATGCAT 3 cut(s) 163, 480, 1008
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.