RLG00000019713

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0003526
Physical Location & Seq
Reverse (-)
55878631 .. 55881569
2939 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019713

Sequence Viewer

Length: 1146 bp
ATGTTTGGGTTCGGGAGGCCTAAATTTGTCACTAAATGCAAATCTGATTTAAAGGGCATGAAGGTGAGGCTTGAGACGATTAAGAAGAAGAGGAACTCTGTGCTCAAGTATTTGAAAAATGATGTTGCCGAGCTTCTCAAGAATGGCCTTGACATTAATGCATATGGAAGGGCTGAGGGAGTTCTGGTTGAGCAAAATATGTCCGCCTGTTATGCACTGACTGAGAACTTTCTTGACTGTATCGCAAATCATCTTTCTCTCATGCAAAATCAGAGGTATTTTGCAGATAAATTCAAGTCAAAGGCCCCTACAAAGGAGATGAAGGTACAATTAATGCACGACATAGCGAGAGAATACTCAATCGAGTGGGACTCTAAGGCCCTGGAACAAAAGCTGTTTACTCCACCTCCAACAAACAAGGGGCAGCCCGCATATGGGTCTCCATGTGACGGTGATGATGATATGTACAGATCACAGAAGAAGGATAACGCTCTTCTGAAGAGAAAAAATAAGGGTGATGAAGACAAACGGAGCTATGTAACTGATGATACTGCATCAGTAGGAAGTGATGATGAAGCAACAGATATGTCCCATGATGGCCGAATGACTGCTTCAAGTTCAGTAGGAAGTAGATCCGAGGATGAAGCAGAAAACAAGGTGCCCTCTTCCTATAAGTTCACTGCACCCCCTTACTACAAACCAAATCCTCAGAAAAAAGAAAGTGTCTTTGAGGAACCCAGGAAGGTGAATGGTCATGCTGAACGAAGGTCAGTTAGGAGAACCTCGAAACCACCAGCAGGTGGTGACAATCATGGAAGTGATCCGAGGGATGAAGAAGAGAGGGTAATGGATGGGCTTTTGATGCATTACAGTCATAAACAGTCACCCTATGACTCAAGCAAGATGAAGGGAAATCTAAAACCATGCTACAACCAGTCAGATGAAGATACTAATGAATCCACAAGGCACAGAAGCAGCAGGTCTGAAAGAGCAGCATCTCTGCCACCACCAGAGCAATCAACTTCACCAGAGCCAACAAAAAGGCATGCTCGAGGCAATTCCTTACAACCAGATGCCCATGTGCACCCGAACCTACCGGAGTATGATGAATTGGCAGCCCGAATTGCAGCTCTTAGAAGAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

382

Amino Acids

43.04

Weight (kDa)

8.8

Isoelectric Point (pI)

62.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 14 - 93 2.9e-16 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 788
Acc36I ACCTGC 2 cut(s) 788, 969
AccB1I GGYRCC 1 cut(s) 658
AccB7I CCANNNNNTGG 1 cut(s) 800
AciI CCGC 2 cut(s) 204, 429
AclWI GGATC 2 cut(s) 627, 815
AcoI YGGCCR 1 cut(s) 598
AcsI RAATTY 2 cut(s) 23, 290
AcuI CTGAAG 1 cut(s) 518
AfaI GTAC 2 cut(s) 327, 467
AfiI CCNNNNNNNGG 6 cut(s) 313, 434, 435, 449, 797, 800
AgsI TTSAA 3 cut(s) 115, 295, 615
AjnI CCWGG 2 cut(s) 381, 737
AluBI AGCT 4 cut(s) 133, 394, 534, 1130
AluI AGCT 4 cut(s) 133, 394, 534, 1130
Alw21I GWGCWC 2 cut(s) 105, 1086
Alw26I GTCTC 2 cut(s) 68, 444
Alw44I GTGCAC 1 cut(s) 1082
AlwI GGATC 2 cut(s) 627, 815
Ama87I CYCGRG 1 cut(s) 1050
AoxI GGCC 5 cut(s) 17, 145, 303, 378, 598
ApaLI GTGCAC 1 cut(s) 1082
ApeKI GCWGC 5 cut(s) 424, 975, 992, 1115, 1127
ApoI RAATTY 2 cut(s) 23, 290
AseI ATTAAT 2 cut(s) 156, 332
AspS9I GGNCC 2 cut(s) 304, 379
AsuHPI GGTGA 7 cut(s) 76, 464, 527, 757, 815, 876, 1017
AvaI CYCGRG 1 cut(s) 1050
BaeGI GKGCMC 2 cut(s) 663, 1086
BanI GGYRCC 1 cut(s) 658
BbsI GAAGAC 1 cut(s) 528
Bbv12I GWGCWC 2 cut(s) 105, 1086
BbvCI CCTCAGC 1 cut(s) 174
BbvI GCAGC 5 cut(s) 436, 987, 1004, 1127, 1139
BccI CCATC 2 cut(s) 590, 845
BciT130I CCWGG 2 cut(s) 383, 739
BcoDI GTCTC 2 cut(s) 68, 444
BfuAI ACCTGC 2 cut(s) 788, 969
BisI GCNGC 5 cut(s) 425, 976, 993, 1116, 1128
BlsI GCNGC 5 cut(s) 426, 977, 994, 1117, 1129
Bme1390I CCNGG 2 cut(s) 383, 739
BmeT110I CYCGRG 1 cut(s) 1050
BmgT120I GGNCC 2 cut(s) 304, 379
BmiI GGNNCC 3 cut(s) 306, 660, 735
BmrFI CCNGG 2 cut(s) 383, 739
BmsI GCATC 4 cut(s) 563, 852, 1004, 1063
BpiI GAAGAC 1 cut(s) 528
BplI GAGNNNNNCTC 2 cut(s) 356, 388
Bpu10I CCTNAGC 1 cut(s) 174
BpuEI CTTGAG 4 cut(s) 89, 92, 122, 880
BsaI GGTCTC 1 cut(s) 444
BsaJI CCNNGG 4 cut(s) 381, 636, 737, 824
BsaWI WCCGGW 1 cut(s) 1096
BsaXI ACNNNNNCTCC 4 cut(s) 171, 201, 391, 421
Bsc4I CCNNNNNNNGG 6 cut(s) 313, 434, 435, 449, 797, 800
Bse1I ACTGG 1 cut(s) 934
BseBI CCWGG 2 cut(s) 383, 739
BseDI CCNNGG 4 cut(s) 381, 636, 737, 824
BseGI GGATG 3 cut(s) 646, 835, 856
BseLI CCNNNNNNNGG 6 cut(s) 313, 434, 435, 449, 797, 800
BseMII CTCAG 3 cut(s) 165, 213, 722
BseNI ACTGG 1 cut(s) 934
BseSI GKGCMC 2 cut(s) 663, 1086
BseXI GCAGC 5 cut(s) 436, 987, 1004, 1127, 1139
BsgI GTGCAG 1 cut(s) 666
BshFI GGCC 5 cut(s) 19, 147, 305, 380, 600
BshNI GGYRCC 1 cut(s) 658
BsiHKAI GWGCWC 2 cut(s) 105, 1086
BsiHKCI CYCGRG 1 cut(s) 1050
BsiSI CCGG 1 cut(s) 1097
BslFI GGGAC 2 cut(s) 383, 574
BslI CCNNNNNNNGG 6 cut(s) 313, 434, 435, 449, 797, 800
BsmAI GTCTC 2 cut(s) 68, 444
BsmBI CGTCTC 1 cut(s) 68
BsmFI GGGAC 2 cut(s) 383, 574
BsnI GGCC 5 cut(s) 19, 147, 305, 380, 600
Bso31I GGTCTC 1 cut(s) 444
BsoBI CYCGRG 1 cut(s) 1050
Bsp1286I GDGCHC 3 cut(s) 105, 663, 1086
Bsp1407I TGTACA 1 cut(s) 465
Bsp143I GATC 3 cut(s) 470, 632, 820
BspACI CCGC 2 cut(s) 204, 429
BspANI GGCC 5 cut(s) 19, 147, 305, 380, 600
BspCNI CTCAG 3 cut(s) 166, 214, 721
BspLI GGNNCC 3 cut(s) 306, 660, 735
BspMI ACCTGC 2 cut(s) 788, 969
BspPI GGATC 2 cut(s) 627, 815
BspQI GCTCTTC 1 cut(s) 498
BspT107I GGYRCC 1 cut(s) 658
BspTNI GGTCTC 1 cut(s) 444
BsrGI TGTACA 1 cut(s) 465
BsrI ACTGG 1 cut(s) 934
BssECI CCNNGG 4 cut(s) 381, 636, 737, 824
BssMI GATC 3 cut(s) 470, 632, 820
Bst2UI CCWGG 2 cut(s) 383, 739
Bst4CI ACNGT 4 cut(s) 239, 452, 872, 882
Bst6I CTCTTC 5 cut(s) 83, 494, 498, 670, 831
BstAUI TGTACA 1 cut(s) 465
BstC8I GCNNGC 2 cut(s) 429, 1047
BstDEI CTNAG 5 cut(s) 174, 222, 375, 708, 1133
BstF5I GGATG 3 cut(s) 646, 835, 856
BstKTI GATC 3 cut(s) 473, 635, 823
BstMAI GTCTC 2 cut(s) 68, 444
BstMBI GATC 3 cut(s) 470, 632, 820
BstMWI GCNNNNNNNGC 3 cut(s) 212, 862, 1124
BstNI CCWGG 2 cut(s) 383, 739
BstNSI RCATGY 1 cut(s) 1049
BstSCI CCNGG 2 cut(s) 381, 737
BstSLI GKGCMC 2 cut(s) 663, 1086
BstV1I GCAGC 5 cut(s) 436, 987, 1004, 1127, 1139
BstV2I GAAGAC 1 cut(s) 528
BstX2I RGATCY 1 cut(s) 632
BstYI RGATCY 1 cut(s) 632
BsuRI GGCC 5 cut(s) 19, 147, 305, 380, 600
BtsCI GGATG 3 cut(s) 646, 835, 856
BtsI GCAGTG 1 cut(s) 678
BtsIMutI CAGTG 2 cut(s) 215, 678
BveI ACCTGC 2 cut(s) 788, 969
Cac8I GCNNGC 2 cut(s) 429, 1047
Cfr13I GGNCC 2 cut(s) 304, 379
Csp6I GTAC 2 cut(s) 326, 466
CviQI GTAC 2 cut(s) 326, 466
DdeI CTNAG 5 cut(s) 174, 222, 375, 708, 1133
DpnI GATC 3 cut(s) 472, 634, 822
DpnII GATC 3 cut(s) 470, 632, 820
DraI TTTAAA 1 cut(s) 51
EaeI YGGCCR 1 cut(s) 598
Eam1104I CTCTTC 5 cut(s) 83, 494, 498, 670, 831
EarI CTCTTC 5 cut(s) 83, 494, 498, 670, 831
EciI GGCGGA 1 cut(s) 193
Eco147I AGGCCT 1 cut(s) 19
Eco31I GGTCTC 1 cut(s) 444
Eco57I CTGAAG 1 cut(s) 518
Eco88I CYCGRG 1 cut(s) 1050
EcoO109I RGGNCCY 2 cut(s) 304, 379
EcoRII CCWGG 2 cut(s) 381, 737
EcoT22I ATGCAT 2 cut(s) 163, 867
Esp3I CGTCTC 1 cut(s) 68
FaqI GGGAC 2 cut(s) 383, 574
FauI CCCGC 1 cut(s) 436
FauNDI CATATG 2 cut(s) 163, 433
Fnu4HI GCNGC 5 cut(s) 425, 976, 993, 1116, 1128
FokI GGATG 3 cut(s) 653, 842, 863
Fsp4HI GCNGC 5 cut(s) 425, 976, 993, 1116, 1128
GluI GCNGC 5 cut(s) 425, 976, 993, 1116, 1128
HaeIII GGCC 5 cut(s) 19, 147, 305, 380, 600
HapII CCGG 1 cut(s) 1097
HinfI GANTC 3 cut(s) 371, 893, 956
HpaII CCGG 1 cut(s) 1097
HphI GGTGA 7 cut(s) 76, 464, 527, 757, 815, 876, 1017
Hpy166II GTNNAC 3 cut(s) 399, 678, 1084
Hpy188I TCNGA 8 cut(s) 46, 273, 498, 637, 711, 825, 940, 985
Hpy188III TCNNGA 3 cut(s) 13, 139, 233
Hpy8I GTNNAC 3 cut(s) 399, 678, 1084
HpyAV CCTTC 7 cut(s) 55, 162, 316, 475, 736, 759, 901
HpyCH4III ACNGT 4 cut(s) 239, 452, 872, 882
HpyF10VI GCNNNNNNNGC 3 cut(s) 212, 862, 1124
HpyF3I CTNAG 5 cut(s) 174, 222, 375, 708, 1133
Kzo9I GATC 3 cut(s) 470, 632, 820
LguI GCTCTTC 1 cut(s) 498
LmnI GCTCC 1 cut(s) 531
Lsp1109I GCAGC 5 cut(s) 436, 987, 1004, 1127, 1139
LweI GCATC 4 cut(s) 563, 852, 1004, 1063
MaeIII GTNAC 5 cut(s) 28, 446, 538, 803, 882
MalI GATC 3 cut(s) 472, 634, 822
MboI GATC 3 cut(s) 470, 632, 820
MflI RGATCY 1 cut(s) 632
MhlI GDGCHC 3 cut(s) 105, 663, 1086
MluCI AATT 6 cut(s) 23, 290, 329, 1057, 1109, 1122
MlyI GAGTC 2 cut(s) 365, 887
MmeI TCCRAC 1 cut(s) 434
Mph1103I ATGCAT 2 cut(s) 163, 867
MseI TTAA 4 cut(s) 50, 81, 156, 332
MslI CAYNNNNRTG 2 cut(s) 62, 816
MspI CCGG 1 cut(s) 1097
MspR9I CCNGG 2 cut(s) 383, 739
MvaI CCWGG 2 cut(s) 383, 739
MwoI GCNNNNNNNGC 3 cut(s) 212, 862, 1124
NdeI CATATG 2 cut(s) 163, 433
NdeII GATC 3 cut(s) 470, 632, 820
NlaIV GGNNCC 3 cut(s) 306, 660, 735
NmeAIII GCCGAG 1 cut(s) 154
NmuCI GTSAC 4 cut(s) 28, 446, 803, 882
NsiI ATGCAT 2 cut(s) 163, 867
NspI RCATGY 1 cut(s) 1049
PaeI GCATGC 1 cut(s) 1049
PaeR7I CTCGAG 1 cut(s) 1050
PaqCI CACCTGC 1 cut(s) 788
PceI AGGCCT 1 cut(s) 19
PciSI GCTCTTC 1 cut(s) 498
PfeI GAWTC 1 cut(s) 956
PflMI CCANNNNNTGG 1 cut(s) 800
PkrI GCNGC 5 cut(s) 426, 977, 994, 1117, 1129
PleI GAGTC 2 cut(s) 365, 887
PpsI GAGTC 2 cut(s) 365, 887
PshBI ATTAAT 2 cut(s) 156, 332
Psp6I CCWGG 2 cut(s) 381, 737
PspGI CCWGG 2 cut(s) 381, 737
PspN4I GGNNCC 3 cut(s) 306, 660, 735
PspPI GGNCC 2 cut(s) 304, 379
PspXI VCTCGAGB 1 cut(s) 1050
PsuI RGATCY 1 cut(s) 632
RsaI GTAC 2 cut(s) 327, 467
RsaNI GTAC 2 cut(s) 326, 466
RseI CAYNNNNRTG 2 cut(s) 62, 816
SapI GCTCTTC 1 cut(s) 498
SaqAI TTAA 4 cut(s) 50, 81, 156, 332
SatI GCNGC 5 cut(s) 425, 976, 993, 1116, 1128
Sau3AI GATC 3 cut(s) 470, 632, 820
Sau96I GGNCC 2 cut(s) 304, 379
SchI GAGTC 2 cut(s) 365, 887
ScrFI CCNGG 2 cut(s) 383, 739
SduI GDGCHC 3 cut(s) 105, 663, 1086
SfaNI GCATC 4 cut(s) 563, 852, 1004, 1063
Sfr274I CTCGAG 1 cut(s) 1050
SlaI CTCGAG 1 cut(s) 1050
SmiMI CAYNNNNRTG 2 cut(s) 62, 816
SmlI CTYRAG 5 cut(s) 71, 104, 137, 895, 1050
SmoI CTYRAG 5 cut(s) 71, 104, 137, 895, 1050
SphI GCATGC 1 cut(s) 1049
Sse9I AATT 6 cut(s) 23, 290, 329, 1057, 1109, 1122
SseBI AGGCCT 1 cut(s) 19
SsiI CCGC 2 cut(s) 204, 429
StuI AGGCCT 1 cut(s) 19
StyD4I CCNGG 2 cut(s) 381, 737
TaaI ACNGT 4 cut(s) 239, 452, 872, 882
TaqI TCGA 3 cut(s) 363, 785, 1051
TasI AATT 6 cut(s) 23, 290, 329, 1057, 1109, 1122
TatI WGTACW 1 cut(s) 465
TfiI GAWTC 1 cut(s) 956
Tru1I TTAA 4 cut(s) 50, 81, 156, 332
Tru9I TTAA 4 cut(s) 50, 81, 156, 332
TscAI CASTG 2 cut(s) 222, 685
TseFI GTSAC 4 cut(s) 28, 446, 803, 882
TseI GCWGC 5 cut(s) 424, 975, 992, 1115, 1127
Tsp45I GTSAC 4 cut(s) 28, 446, 803, 882
TspGWI ACGGA 1 cut(s) 544
TspRI CASTG 2 cut(s) 222, 685
Van91I CCANNNNNTGG 1 cut(s) 800
VneI GTGCAC 1 cut(s) 1082
VspI ATTAAT 2 cut(s) 156, 332
XapI RAATTY 2 cut(s) 23, 290
XceI RCATGY 1 cut(s) 1049
XhoI CTCGAG 1 cut(s) 1050
Zsp2I ATGCAT 2 cut(s) 163, 867
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.