Rh2BG410900

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0003526
Physical Location & Seq
Forward (+)
58139733 .. 58144449
4717 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG410900.1

Sequence Viewer

Length: 1149 bp
ATGCTCTTAACTTTTCATAAATTGGTGCAACAGGCTGAGGGAGTTATGGTTGAGCAAAATATGTCTGCCTGTTATGCACTGACTGAGAACTTTCTTGACTGTATCGCGAATCATCTTTCTCTCATGCAAAATCAGAGGGAATGTCCTGAAGAATGCAAGGAAGCTGTTGCATCTGTGATATATGCTGCAGCAAGATTTTCTGATTTGCCAGAACTGCGTGACCTCAGAAACATATTTACTGGAAAGTATGGAGATTCCCTACAATCTTTTGTAAATAAAGAGTTTGCGGATAAATTCAAGTCAAAGGCCCCTACAAAGGAGATGAAGGTACAATTAATGCATGACATAGCGAAAGAATACTCAATCGAGTGGGACTCCAAGGCTCTGGAACAAAAGCTGTTTACTCCACCTCCAACAAACAAGGGGCAGCCTACGTATGGCTCTCCGTGTGACAGTGATGATGATATGTACAGATCACAGAAGAAGGATAACGCTCTTCCGAAGAGAAAAAATAAGGGTGATGGAGACAAACGGAGCTATGTAACTGATGATACTGCATCAGTAGGAAGTGATGATGAAGCAACAGATATGTCCCATGATGACCGAATGACTGCTTCAAGTTCAGTAGGAAGTAGATCCGAGGATGAAGCAGAAAACAAGGTGCCCTCTTCCTATAAGTTCACTGCACCCCCTTACTACAAACCAAAGCCTCAGAAAAAAGAAAGTGTCTTTGAGGAACCCAGGAAGGTGAATGGTCATGCTGAACGAAAGTCAGTTAGGAGAACCTCGAAACCACCAGCAGGTGGTGACAATCATGGAAGTGATCCGAGGGATGAAGAAGAGAGGGTAATGGACGGGCTTTTGATGCATTACAGTCATAAACAGTCACCCTATGACGCAAGCAAGATGAAGGGAAATCTAAAACCTTGCTACAACCAGTCAGATGAAAATACTAATGAATCCACAAGGCACAGAAGCAGCAGGTCTGAAAGAGCAGCATCTCTGCCACCACCAGAGCAATCAACTTCACCTGAGCCAACAAAAAGGCATGCTCGAGGCAATTCCTTACAACCAGATGCCCATGTGCACCCGAACCTACCGGAGTATGATGAATTGGCAGCCCGAATTGCAGCTCTTAGAGGAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

382

Amino Acids

42.96

Weight (kDa)

6.21

Isoelectric Point (pI)

68.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 11 - 123 2.4e-33 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 791
Acc36I ACCTGC 2 cut(s) 791, 972
AccB1I GGYRCC 1 cut(s) 661
AccB7I CCANNNNNTGG 1 cut(s) 803
AccII CGCG 1 cut(s) 107
AciI CCGC 1 cut(s) 287
AclWI GGATC 2 cut(s) 630, 818
AcsI RAATTY 1 cut(s) 293
AcuI CTGAAG 1 cut(s) 168
AfaI GTAC 2 cut(s) 330, 470
AfiI CCNNNNNNNGG 4 cut(s) 316, 437, 800, 803
AgsI TTSAA 2 cut(s) 298, 618
AjnI CCWGG 1 cut(s) 740
AluBI AGCT 4 cut(s) 164, 397, 537, 1133
AluI AGCT 4 cut(s) 164, 397, 537, 1133
Alw21I GWGCWC 1 cut(s) 1089
Alw26I GTCTC 1 cut(s) 519
Alw44I GTGCAC 1 cut(s) 1085
AlwI GGATC 2 cut(s) 630, 818
Ama87I CYCGRG 1 cut(s) 1053
AoxI GGCC 1 cut(s) 306
ApaLI GTGCAC 1 cut(s) 1085
ApeKI GCWGC 7 cut(s) 185, 188, 427, 978, 995, 1118, 1130
ApoI RAATTY 1 cut(s) 293
AseI ATTAAT 1 cut(s) 335
AspS9I GGNCC 1 cut(s) 307
AsuHPI GGTGA 5 cut(s) 530, 760, 818, 879, 1020
AvaI CYCGRG 1 cut(s) 1053
BaeGI GKGCMC 2 cut(s) 666, 1089
BanI GGYRCC 1 cut(s) 661
Bbv12I GWGCWC 1 cut(s) 1089
BbvCI CCTCAGC 1 cut(s) 36
BbvI GCAGC 7 cut(s) 172, 200, 439, 990, 1007, 1130, 1142
BccI CCATC 1 cut(s) 515
BciT130I CCWGG 1 cut(s) 742
BcoDI GTCTC 1 cut(s) 519
BfmI CTRYAG 1 cut(s) 186
BfuAI ACCTGC 2 cut(s) 791, 972
BisI GCNGC 7 cut(s) 186, 189, 428, 979, 996, 1119, 1131
BlsI GCNGC 7 cut(s) 187, 190, 429, 980, 997, 1120, 1132
Bme1390I CCNGG 1 cut(s) 742
BmeT110I CYCGRG 1 cut(s) 1053
BmgT120I GGNCC 1 cut(s) 307
BmiI GGNNCC 3 cut(s) 309, 663, 738
BmrFI CCNGG 1 cut(s) 742
BmsI GCATC 5 cut(s) 179, 566, 855, 1007, 1066
BplI GAGNNNNNCTC 2 cut(s) 359, 391
Bpu10I CCTNAGC 2 cut(s) 36, 1032
BsaAI YACGTR 1 cut(s) 435
BsaJI CCNNGG 4 cut(s) 378, 639, 740, 827
BsaWI WCCGGW 1 cut(s) 1099
BsaXI ACNNNNNCTCC 4 cut(s) 33, 63, 394, 424
Bsc4I CCNNNNNNNGG 4 cut(s) 316, 437, 800, 803
Bse1I ACTGG 2 cut(s) 244, 937
BseBI CCWGG 1 cut(s) 742
BseDI CCNNGG 4 cut(s) 378, 639, 740, 827
BseGI GGATG 2 cut(s) 649, 838
BseLI CCNNNNNNNGG 4 cut(s) 316, 437, 800, 803
BseMII CTCAG 5 cut(s) 27, 75, 238, 725, 1023
BseNI ACTGG 2 cut(s) 244, 937
BseSI GKGCMC 2 cut(s) 666, 1089
BseXI GCAGC 7 cut(s) 172, 200, 439, 990, 1007, 1130, 1142
BsgI GTGCAG 1 cut(s) 669
Bsh1236I CGCG 1 cut(s) 107
BshFI GGCC 1 cut(s) 308
BshNI GGYRCC 1 cut(s) 661
BsiHKAI GWGCWC 1 cut(s) 1089
BsiHKCI CYCGRG 1 cut(s) 1053
BsiSI CCGG 1 cut(s) 1100
BslFI GGGAC 2 cut(s) 386, 577
BslI CCNNNNNNNGG 4 cut(s) 316, 437, 800, 803
BsmAI GTCTC 1 cut(s) 519
BsmFI GGGAC 2 cut(s) 386, 577
BsmI GAATGC 1 cut(s) 158
BsnI GGCC 1 cut(s) 308
BsoBI CYCGRG 1 cut(s) 1053
Bsp1286I GDGCHC 2 cut(s) 666, 1089
Bsp1407I TGTACA 1 cut(s) 468
Bsp143I GATC 3 cut(s) 473, 635, 823
Bsp68I TCGCGA 1 cut(s) 107
BspACI CCGC 1 cut(s) 287
BspANI GGCC 1 cut(s) 308
BspCNI CTCAG 5 cut(s) 28, 76, 237, 724, 1024
BspFNI CGCG 1 cut(s) 107
BspLI GGNNCC 3 cut(s) 309, 663, 738
BspMAI CTGCAG 1 cut(s) 190
BspMI ACCTGC 2 cut(s) 791, 972
BspPI GGATC 2 cut(s) 630, 818
BspQI GCTCTTC 1 cut(s) 501
BspT107I GGYRCC 1 cut(s) 661
BsrGI TGTACA 1 cut(s) 468
BsrI ACTGG 2 cut(s) 244, 937
BssECI CCNNGG 4 cut(s) 378, 639, 740, 827
BssMI GATC 3 cut(s) 473, 635, 823
BssT1I CCWWGG 1 cut(s) 378
Bst2UI CCWGG 1 cut(s) 742
Bst4CI ACNGT 4 cut(s) 101, 455, 875, 885
Bst6I CTCTTC 4 cut(s) 497, 501, 673, 834
BstAUI TGTACA 1 cut(s) 468
BstBAI YACGTR 1 cut(s) 435
BstC8I GCNNGC 2 cut(s) 901, 1050
BstDEI CTNAG 6 cut(s) 36, 84, 224, 711, 1032, 1136
BstF5I GGATG 2 cut(s) 649, 838
BstFNI CGCG 1 cut(s) 107
BstKTI GATC 3 cut(s) 476, 638, 826
BstMAI GTCTC 1 cut(s) 519
BstMBI GATC 3 cut(s) 473, 635, 823
BstMWI GCNNNNNNNGC 4 cut(s) 74, 214, 865, 1127
BstNI CCWGG 1 cut(s) 742
BstNSI RCATGY 1 cut(s) 1052
BstSCI CCNGG 1 cut(s) 740
BstSFI CTRYAG 1 cut(s) 186
BstSLI GKGCMC 2 cut(s) 666, 1089
BstSNI TACGTA 1 cut(s) 435
BstUI CGCG 1 cut(s) 107
BstV1I GCAGC 7 cut(s) 172, 200, 439, 990, 1007, 1130, 1142
BstX2I RGATCY 1 cut(s) 635
BstXI CCANNNNNNTGG 1 cut(s) 385
BstYI RGATCY 1 cut(s) 635
BsuRI GGCC 1 cut(s) 308
BtsCI GGATG 2 cut(s) 649, 838
BtsI GCAGTG 1 cut(s) 681
BtsIMutI CAGTG 3 cut(s) 77, 460, 681
BtuMI TCGCGA 1 cut(s) 107
BveI ACCTGC 2 cut(s) 791, 972
Cac8I GCNNGC 2 cut(s) 901, 1050
Cfr13I GGNCC 1 cut(s) 307
CseI GACGC 1 cut(s) 905
Csp6I GTAC 2 cut(s) 329, 469
CviAII CATG 8 cut(s) 124, 341, 596, 758, 815, 1049, 1082, 1146
CviQI GTAC 2 cut(s) 329, 469
DdeI CTNAG 6 cut(s) 36, 84, 224, 711, 1032, 1136
DpnI GATC 3 cut(s) 475, 637, 825
DpnII GATC 3 cut(s) 473, 635, 823
Eam1104I CTCTTC 4 cut(s) 497, 501, 673, 834
EarI CTCTTC 4 cut(s) 497, 501, 673, 834
Eco105I TACGTA 1 cut(s) 435
Eco130I CCWWGG 1 cut(s) 378
Eco57I CTGAAG 1 cut(s) 168
Eco88I CYCGRG 1 cut(s) 1053
EcoO109I RGGNCCY 1 cut(s) 307
EcoRII CCWGG 1 cut(s) 740
EcoT14I CCWWGG 1 cut(s) 378
EcoT22I ATGCAT 2 cut(s) 342, 870
ErhI CCWWGG 1 cut(s) 378
FaeI CATG 8 cut(s) 127, 344, 599, 761, 818, 1052, 1085, 1149
FaqI GGGAC 2 cut(s) 386, 577
FatI CATG 8 cut(s) 123, 340, 595, 757, 814, 1048, 1081, 1145
Fnu4HI GCNGC 7 cut(s) 186, 189, 428, 979, 996, 1119, 1131
FokI GGATG 2 cut(s) 656, 845
Fsp4HI GCNGC 7 cut(s) 186, 189, 428, 979, 996, 1119, 1131
GluI GCNGC 7 cut(s) 186, 189, 428, 979, 996, 1119, 1131
HaeIII GGCC 1 cut(s) 308
HapII CCGG 1 cut(s) 1100
HgaI GACGC 1 cut(s) 905
Hin1II CATG 8 cut(s) 127, 344, 599, 761, 818, 1052, 1085, 1149
HinfI GANTC 4 cut(s) 109, 254, 374, 959
HpaII CCGG 1 cut(s) 1100
HphI GGTGA 5 cut(s) 530, 760, 818, 879, 1020
Hpy166II GTNNAC 3 cut(s) 402, 681, 1087
Hpy188I TCNGA 9 cut(s) 135, 202, 227, 501, 640, 714, 828, 943, 988
Hpy188III TCNNGA 4 cut(s) 95, 106, 146, 386
Hpy8I GTNNAC 3 cut(s) 402, 681, 1087
HpyAV CCTTC 4 cut(s) 319, 478, 739, 904
HpyCH4III ACNGT 4 cut(s) 101, 455, 875, 885
HpyCH4IV ACGT 1 cut(s) 434
HpyF10VI GCNNNNNNNGC 4 cut(s) 74, 214, 865, 1127
HpyF3I CTNAG 6 cut(s) 36, 84, 224, 711, 1032, 1136
HpySE526I ACGT 1 cut(s) 434
Hsp92II CATG 8 cut(s) 127, 344, 599, 761, 818, 1052, 1085, 1149
Kzo9I GATC 3 cut(s) 473, 635, 823
LguI GCTCTTC 1 cut(s) 501
LmnI GCTCC 1 cut(s) 534
Lsp1109I GCAGC 7 cut(s) 172, 200, 439, 990, 1007, 1130, 1142
LweI GCATC 5 cut(s) 179, 566, 855, 1007, 1066
MaeII ACGT 1 cut(s) 434
MaeIII GTNAC 5 cut(s) 218, 449, 541, 806, 885
MalI GATC 3 cut(s) 475, 637, 825
MboI GATC 3 cut(s) 473, 635, 823
MboII GAAGA 7 cut(s) 161, 488, 493, 514, 660, 848, 851
MflI RGATCY 1 cut(s) 635
MhlI GDGCHC 2 cut(s) 666, 1089
MluCI AATT 6 cut(s) 20, 293, 332, 1060, 1112, 1125
MlyI GAGTC 1 cut(s) 368
MmeI TCCRAC 1 cut(s) 437
Mph1103I ATGCAT 2 cut(s) 342, 870
MseI TTAA 2 cut(s) 8, 335
MslI CAYNNNNRTG 1 cut(s) 819
MspI CCGG 1 cut(s) 1100
MspR9I CCNGG 1 cut(s) 742
Mva1269I GAATGC 1 cut(s) 158
MvaI CCWGG 1 cut(s) 742
MvnI CGCG 1 cut(s) 107
MwoI GCNNNNNNNGC 4 cut(s) 74, 214, 865, 1127
NdeII GATC 3 cut(s) 473, 635, 823
NlaIII CATG 8 cut(s) 127, 344, 599, 761, 818, 1052, 1085, 1149
NlaIV GGNNCC 3 cut(s) 309, 663, 738
NmuCI GTSAC 4 cut(s) 218, 449, 806, 885
NruI TCGCGA 1 cut(s) 107
NsiI ATGCAT 2 cut(s) 342, 870
NspI RCATGY 1 cut(s) 1052
PaeI GCATGC 1 cut(s) 1052
PaeR7I CTCGAG 1 cut(s) 1053
PaqCI CACCTGC 1 cut(s) 791
PciSI GCTCTTC 1 cut(s) 501
PctI GAATGC 1 cut(s) 158
PfeI GAWTC 3 cut(s) 109, 254, 959
PflMI CCANNNNNTGG 1 cut(s) 803
PkrI GCNGC 7 cut(s) 187, 190, 429, 980, 997, 1120, 1132
PleI GAGTC 1 cut(s) 368
PpsI GAGTC 1 cut(s) 368
Ppu21I YACGTR 1 cut(s) 435
PshBI ATTAAT 1 cut(s) 335
Psp6I CCWGG 1 cut(s) 740
PspGI CCWGG 1 cut(s) 740
PspN4I GGNNCC 3 cut(s) 309, 663, 738
PspPI GGNCC 1 cut(s) 307
PspXI VCTCGAGB 1 cut(s) 1053
PstI CTGCAG 1 cut(s) 190
PsuI RGATCY 1 cut(s) 635
RruI TCGCGA 1 cut(s) 107
RsaI GTAC 2 cut(s) 330, 470
RsaNI GTAC 2 cut(s) 329, 469
RseI CAYNNNNRTG 1 cut(s) 819
SapI GCTCTTC 1 cut(s) 501
SaqAI TTAA 2 cut(s) 8, 335
SatI GCNGC 7 cut(s) 186, 189, 428, 979, 996, 1119, 1131
Sau3AI GATC 3 cut(s) 473, 635, 823
Sau96I GGNCC 1 cut(s) 307
SchI GAGTC 1 cut(s) 368
ScrFI CCNGG 1 cut(s) 742
SduI GDGCHC 2 cut(s) 666, 1089
SfaNI GCATC 5 cut(s) 179, 566, 855, 1007, 1066
SfcI CTRYAG 1 cut(s) 186
Sfr274I CTCGAG 1 cut(s) 1053
SlaI CTCGAG 1 cut(s) 1053
SmiMI CAYNNNNRTG 1 cut(s) 819
SmlI CTYRAG 1 cut(s) 1053
SmoI CTYRAG 1 cut(s) 1053
SnaBI TACGTA 1 cut(s) 435
SphI GCATGC 1 cut(s) 1052
Sse9I AATT 6 cut(s) 20, 293, 332, 1060, 1112, 1125
SsiI CCGC 1 cut(s) 287
StyD4I CCNGG 1 cut(s) 740
StyI CCWWGG 1 cut(s) 378
TaaI ACNGT 4 cut(s) 101, 455, 875, 885
TaiI ACGT 1 cut(s) 437
TaqI TCGA 3 cut(s) 366, 788, 1054
TaqII GACCGA 1 cut(s) 618
TasI AATT 6 cut(s) 20, 293, 332, 1060, 1112, 1125
TatI WGTACW 1 cut(s) 468
TfiI GAWTC 3 cut(s) 109, 254, 959
Tru1I TTAA 2 cut(s) 8, 335
Tru9I TTAA 2 cut(s) 8, 335
TscAI CASTG 3 cut(s) 84, 460, 688
TseFI GTSAC 4 cut(s) 218, 449, 806, 885
TseI GCWGC 7 cut(s) 185, 188, 427, 978, 995, 1118, 1130
Tsp45I GTSAC 4 cut(s) 218, 449, 806, 885
TspDTI ATGAA 9 cut(s) 5, 338, 591, 660, 849, 923, 960, 972, 1125
TspGWI ACGGA 2 cut(s) 435, 547
TspRI CASTG 3 cut(s) 84, 460, 688
Van91I CCANNNNNTGG 1 cut(s) 803
VneI GTGCAC 1 cut(s) 1085
VspI ATTAAT 1 cut(s) 335
XapI RAATTY 1 cut(s) 293
XceI RCATGY 1 cut(s) 1052
XhoI CTCGAG 1 cut(s) 1053
Zsp2I ATGCAT 2 cut(s) 342, 870
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.