MD09G1212300.v1.1

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0003526
Physical Location & Seq
Forward (+)
20781916 .. 20785273
3358 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1212300.v1.1.491

Sequence Viewer

Length: 1281 bp
ATGCGGATCGAGGCGATAAAGAAGAAGAGGAGCGCGGTGCAGAAGTTTTTGAAGAGCGACATAGCTGATCTTTTGAGGAGTGGCCGTGACATCAATGCATACGGCAGGGCTGAAGGGCTTCTAGTTGAGCAAAATATGTCCGCCTGTTATGAATCAACTGAGAGCTTTCTTGGATGCATCTCGACTCAACTTCCTCTCATACAAAATCAGAGTGAGTGCCCTGAGGAATGCAAGGAAGCTGTTCGGTCAATTATGTACGCTGCAGCACGATTTGCTGATTTACCAGAACTCCGTGAGCTCAGAACTCTGTTTAGTGAAAAGTATGGAAATTCCCTCGAACCTTTTCTGAATAAAGAGTTTGTGGAGAGGTTGAAGTCAAAGCCTCTTACGAAGGAGACGAAGATTCAGTTAATGCACGACATAGCACAAGAATTTTCGATTGAATGGGATTCAAGGTCTTTGGAACAGAAACTGTATACTAATACTCCATCGGCATCTGACCAAGGCCAGCGCAGGTGTGGATCTCCAGTTGACAGCGATGACGATATGCACAAATCACATAAAAGGCAAGACAATGCTTTCCCGAAGAGAAGAAACCAGGAGGGTGACTCTTATGACAGTTACAAGCTGCAGAGCAGCAGTGAAGACGAAACAACAACAGATGTATCCCAATCTGCCCCAAAATATTCTTCAAGCTCAGTAGGAAGTACATCCGGGGATGAAGCAGATACCAGCAAGATGCCCTTCTACTCCAAGTTTGTTCCACCACCTTACTACAGATCAAGACCTGAAAAAGAGGAAAGCAGTCACGTCGAAAGGGAAACAGATCACCCTAGCGATGTTTCAGTTGGTGAAGATAATACAAAACGAAGATCAGCAAGGAGGGGAAACTTGAAACCACCACCAGGTCACGGGAATCTTGGCAGGAATGATAAAACTAGACATGGAGAGGATAGTGATTCAATGGACGAAGAGAAGAGAATAATGGATGGGCTTTTGATACATTACAGCAGGAAACAGTCACCCTATGAATCGAGCCAGATGAAGGGCAGTCTAGACGATGATGATGCTGGTGAATCAACATGGAACAGAAGCAGAAAATCTCAGCGAGAAGCTCCGTCAGCCAGAGCATCATCTCTCCCACCAGAGCCAACAAGTCCAATTGAGGCACCGAAAAAGCATGTCCGAGCATTTTCCTTCACACCAGATGTACACGTGCACCCAAAGCTACCGGAGTACGATGAACTGGCAGCTCGAATTGCCGCTCTTAGAGGAATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

427

Amino Acids

48.15

Weight (kDa)

6.07

Isoelectric Point (pI)

78.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 2 - 147 5.8e-42 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 504
Acc36I ACCTGC 1 cut(s) 504
AccB1I GGYRCC 1 cut(s) 1168
AccBSI CCGCTC 1 cut(s) 1265
AccI GTMKAC 1 cut(s) 476
AccII CGCG 1 cut(s) 35
AciI CCGC 4 cut(s) 4, 35, 141, 1263
AclWI GGATC 2 cut(s) 14, 529
AcoI YGGCCR 1 cut(s) 82
AcsI RAATTY 2 cut(s) 328, 431
AcuI CTGAAG 1 cut(s) 132
AcvI CACGTG 1 cut(s) 1216
AfaI GTAC 4 cut(s) 257, 709, 1212, 1238
AfiI CCNNNNNNNGG 3 cut(s) 905, 911, 1045
AflIII ACRYGT 1 cut(s) 1213
AgsI TTSAA 7 cut(s) 52, 373, 443, 453, 693, 895, 963
AjiI CACGTC 1 cut(s) 811
AjnI CCWGG 2 cut(s) 597, 904
AjuI GAANNNNNNNTTGG 2 cut(s) 673, 705
AluBI AGCT 9 cut(s) 65, 165, 239, 298, 628, 696, 1115, 1228, 1253
AluI AGCT 9 cut(s) 65, 165, 239, 298, 628, 696, 1115, 1228, 1253
Alw21I GWGCWC 2 cut(s) 300, 1221
Alw26I GTCTC 1 cut(s) 389
Alw44I GTGCAC 1 cut(s) 1217
AlwI GGATC 2 cut(s) 14, 529
AlwNI CAGNNNCTG 1 cut(s) 472
AoxI GGCC 2 cut(s) 82, 505
ApaLI GTGCAC 1 cut(s) 1217
ApeKI GCWGC 5 cut(s) 260, 263, 628, 636, 1250
ApoI RAATTY 2 cut(s) 328, 431
Asp700I GAANNNNTTC 3 cut(s) 117, 240, 342
AspLEI GCGC 2 cut(s) 35, 513
AsuC2I CCSGG 1 cut(s) 715
AsuHPI GGTGA 5 cut(s) 617, 821, 863, 1014, 1085
AxyI CCTNAGG 1 cut(s) 222
BaeGI GKGCMC 2 cut(s) 221, 1221
BaeI ACNNNNGTAYC 2 cut(s) 648, 681
BanI GGYRCC 1 cut(s) 1168
BanII GRGCYC 1 cut(s) 300
BbrPI CACGTG 1 cut(s) 1216
BbsI GAAGAC 1 cut(s) 651
Bbv12I GWGCWC 2 cut(s) 300, 1221
BbvI GCAGC 5 cut(s) 247, 275, 615, 648, 1262
BccI CCATC 2 cut(s) 496, 983
BceAI ACGGC 2 cut(s) 69, 118
BcgI CGANNNNNNTGC 4 cut(s) 793, 827, 1049, 1083
BciT130I CCWGG 2 cut(s) 599, 906
BciVI GTATCC 1 cut(s) 676
BcnI CCSGG 1 cut(s) 715
BcoDI GTCTC 1 cut(s) 389
BfaI CTAG 4 cut(s) 122, 834, 939, 1055
BfmI CTRYAG 3 cut(s) 261, 629, 775
BfuAI ACCTGC 1 cut(s) 504
BfuI GTATCC 1 cut(s) 676
BisI GCNGC 6 cut(s) 261, 264, 629, 637, 1251, 1263
BlsI GCNGC 6 cut(s) 262, 265, 630, 638, 1252, 1264
Bme1390I CCNGG 3 cut(s) 599, 715, 906
BmgBI CACGTC 1 cut(s) 811
BmiI GGNNCC 1 cut(s) 1170
BmrFI CCNGG 3 cut(s) 599, 715, 906
BmsI GCATC 6 cut(s) 164, 186, 503, 729, 1057, 1139
BpiI GAAGAC 1 cut(s) 651
BplI GAGNNNNNCTC 2 cut(s) 593, 625
BpmI CTGGAG 1 cut(s) 510
BpuMI CCSGG 1 cut(s) 715
BsaAI YACGTR 1 cut(s) 1216
BsaJI CCNNGG 2 cut(s) 502, 714
BsaWI WCCGGW 1 cut(s) 1231
BsaXI ACNNNNNCTCC 6 cut(s) 70, 100, 273, 303, 469, 499
Bsc4I CCNNNNNNNGG 3 cut(s) 905, 911, 1045
Bse1I ACTGG 2 cut(s) 527, 1251
Bse21I CCTNAGG 1 cut(s) 222
BseBI CCWGG 2 cut(s) 599, 906
BseDI CCNNGG 2 cut(s) 502, 714
BseGI GGATG 4 cut(s) 179, 710, 724, 994
BseLI CCNNNNNNNGG 3 cut(s) 905, 911, 1045
BseMII CTCAG 5 cut(s) 150, 213, 313, 711, 1118
BseNI ACTGG 2 cut(s) 527, 1251
BseRI GAGGAG 2 cut(s) 43, 91
BseSI GKGCMC 2 cut(s) 221, 1221
BseXI GCAGC 5 cut(s) 247, 275, 615, 648, 1262
BsgI GTGCAG 1 cut(s) 59
Bsh1236I CGCG 1 cut(s) 35
BshFI GGCC 2 cut(s) 84, 507
BshNI GGYRCC 1 cut(s) 1168
BsiHKAI GWGCWC 2 cut(s) 300, 1221
BsiSI CCGG 2 cut(s) 714, 1232
BslI CCNNNNNNNGG 3 cut(s) 905, 911, 1045
BsmAI GTCTC 1 cut(s) 389
BsmBI CGTCTC 1 cut(s) 389
BsmI GAATGC 1 cut(s) 233
BsnI GGCC 2 cut(s) 84, 507
Bsp1286I GDGCHC 3 cut(s) 221, 300, 1221
Bsp1407I TGTACA 1 cut(s) 1210
Bsp143I GATC 6 cut(s) 6, 67, 521, 779, 826, 872
BspACI CCGC 4 cut(s) 4, 35, 141, 1263
BspANI GGCC 2 cut(s) 84, 507
BspCNI CTCAG 5 cut(s) 151, 214, 312, 710, 1117
BspFNI CGCG 1 cut(s) 35
BspLI GGNNCC 1 cut(s) 1170
BspMAI CTGCAG 2 cut(s) 265, 633
BspMI ACCTGC 1 cut(s) 504
BspPI GGATC 2 cut(s) 14, 529
BspQI GCTCTTC 1 cut(s) 47
BspT107I GGYRCC 1 cut(s) 1168
BsrBI CCGCTC 1 cut(s) 1265
BsrGI TGTACA 1 cut(s) 1210
BsrI ACTGG 2 cut(s) 527, 1251
BssECI CCNNGG 2 cut(s) 502, 714
BssMI GATC 6 cut(s) 6, 67, 521, 779, 826, 872
BssNAI GTATAC 1 cut(s) 477
BssT1I CCWWGG 1 cut(s) 502
Bst1107I GTATAC 1 cut(s) 477
Bst2UI CCWGG 2 cut(s) 599, 906
Bst4CI ACNGT 3 cut(s) 474, 620, 1020
Bst6I CTCTTC 5 cut(s) 20, 47, 581, 966, 971
BstAPI GCANNNNNTGC 1 cut(s) 272
BstAUI TGTACA 1 cut(s) 1210
BstBAI YACGTR 1 cut(s) 1216
BstC8I GCNNGC 1 cut(s) 509
BstDEI CTNAG 6 cut(s) 159, 222, 299, 697, 1104, 1268
BstF5I GGATG 4 cut(s) 179, 710, 724, 994
BstFNI CGCG 1 cut(s) 35
BstHHI GCGC 2 cut(s) 35, 513
BstKTI GATC 6 cut(s) 9, 70, 524, 782, 829, 875
BstMAI GTCTC 1 cut(s) 389
BstMBI GATC 6 cut(s) 6, 67, 521, 779, 826, 872
BstMWI GCNNNNNNNGC 4 cut(s) 272, 1121, 1225, 1259
BstNI CCWGG 2 cut(s) 599, 906
BstNSI RCATGY 1 cut(s) 1184
BstSCI CCNGG 3 cut(s) 597, 713, 904
BstSFI CTRYAG 3 cut(s) 261, 629, 775
BstSLI GKGCMC 2 cut(s) 221, 1221
BstUI CGCG 1 cut(s) 35
BstV1I GCAGC 5 cut(s) 247, 275, 615, 648, 1262
BstV2I GAAGAC 1 cut(s) 651
BstX2I RGATCY 1 cut(s) 521
BstYI RGATCY 1 cut(s) 521
BstZ17I GTATAC 1 cut(s) 477
Bsu36I CCTNAGG 1 cut(s) 222
BsuI GTATCC 1 cut(s) 676
BsuRI GGCC 2 cut(s) 84, 507
BtgZI GCGATG 2 cut(s) 552, 852
BtrI CACGTC 1 cut(s) 811
BtsCI GGATG 4 cut(s) 179, 710, 724, 994
BtsI GCAGTG 1 cut(s) 646
BtsIMutI CAGTG 1 cut(s) 646
BveI ACCTGC 1 cut(s) 504
Cac8I GCNNGC 1 cut(s) 509
CaiI CAGNNNCTG 1 cut(s) 472
CfoI GCGC 2 cut(s) 35, 513
CsiI ACCWGGT 1 cut(s) 904
Csp6I GTAC 4 cut(s) 256, 708, 1211, 1237
CviAII CATG 3 cut(s) 944, 1083, 1181
CviQI GTAC 4 cut(s) 256, 708, 1211, 1237
DdeI CTNAG 6 cut(s) 159, 222, 299, 697, 1104, 1268
DpnI GATC 6 cut(s) 8, 69, 523, 781, 828, 874
DpnII GATC 6 cut(s) 6, 67, 521, 779, 826, 872
EaeI YGGCCR 1 cut(s) 82
Eam1104I CTCTTC 5 cut(s) 20, 47, 581, 966, 971
EarI CTCTTC 5 cut(s) 20, 47, 581, 966, 971
EciI GGCGGA 1 cut(s) 130
Ecl136II GAGCTC 1 cut(s) 298
Eco130I CCWWGG 1 cut(s) 502
Eco24I GRGCYC 1 cut(s) 300
Eco53kI GAGCTC 1 cut(s) 298
Eco57I CTGAAG 1 cut(s) 132
Eco72I CACGTG 1 cut(s) 1216
Eco81I CCTNAGG 1 cut(s) 222
EcoICRI GAGCTC 1 cut(s) 298
EcoRII CCWGG 2 cut(s) 597, 904
EcoT14I CCWWGG 1 cut(s) 502
EcoT22I ATGCAT 2 cut(s) 100, 179
EcoT38I GRGCYC 1 cut(s) 300
ErhI CCWWGG 1 cut(s) 502
Esp3I CGTCTC 1 cut(s) 389
FaeI CATG 3 cut(s) 947, 1086, 1184
FalI AAGNNNNNCTT 2 cut(s) 728, 760
FatI CATG 3 cut(s) 943, 1082, 1180
FblI GTMKAC 1 cut(s) 476
Fnu4HI GCNGC 6 cut(s) 261, 264, 629, 637, 1251, 1263
FokI GGATG 4 cut(s) 186, 697, 731, 1001
FriOI GRGCYC 1 cut(s) 300
Fsp4HI GCNGC 6 cut(s) 261, 264, 629, 637, 1251, 1263
FspBI CTAG 4 cut(s) 122, 834, 939, 1055
GlaI GCGC 2 cut(s) 34, 512
GluI GCNGC 6 cut(s) 261, 264, 629, 637, 1251, 1263
GsuI CTGGAG 1 cut(s) 510
HaeIII GGCC 2 cut(s) 84, 507
HapII CCGG 2 cut(s) 714, 1232
HhaI GCGC 2 cut(s) 35, 513
Hin1II CATG 3 cut(s) 947, 1086, 1184
Hin6I GCGC 2 cut(s) 33, 511
HinP1I GCGC 2 cut(s) 33, 511
HincII GTYRAC 1 cut(s) 532
HindII GTYRAC 1 cut(s) 532
HinfI GANTC 9 cut(s) 152, 184, 403, 449, 608, 916, 959, 1031, 1076
HpaII CCGG 2 cut(s) 714, 1232
HphI GGTGA 5 cut(s) 617, 821, 863, 1014, 1085
Hpy166II GTNNAC 4 cut(s) 477, 532, 1213, 1219
Hpy188I TCNGA 5 cut(s) 210, 302, 348, 499, 1187
Hpy188III TCNNGA 4 cut(s) 181, 583, 783, 1055
Hpy8I GTNNAC 4 cut(s) 477, 532, 1213, 1219
Hpy99I CGWCG 1 cut(s) 815
HpyAV CCTTC 5 cut(s) 107, 385, 754, 1039, 1207
HpyCH4III ACNGT 3 cut(s) 474, 620, 1020
HpyCH4IV ACGT 2 cut(s) 810, 1215
HpyCH4V TGCA 9 cut(s) 40, 98, 177, 231, 263, 415, 550, 631, 1219
HpyF10VI GCNNNNNNNGC 4 cut(s) 272, 1121, 1225, 1259
HpyF3I CTNAG 6 cut(s) 159, 222, 299, 697, 1104, 1268
HpySE526I ACGT 2 cut(s) 810, 1215
Hsp92II CATG 3 cut(s) 947, 1086, 1184
HspAI GCGC 2 cut(s) 33, 511
Kzo9I GATC 6 cut(s) 6, 67, 521, 779, 826, 872
LguI GCTCTTC 1 cut(s) 47
LmnI GCTCC 2 cut(s) 30, 1120
Lsp1109I GCAGC 5 cut(s) 247, 275, 615, 648, 1262
LweI GCATC 6 cut(s) 164, 186, 503, 729, 1057, 1139
MabI ACCWGGT 1 cut(s) 904
MaeI CTAG 4 cut(s) 122, 834, 939, 1055
MaeII ACGT 2 cut(s) 810, 1215
MaeIII GTNAC 6 cut(s) 86, 605, 620, 806, 908, 1020
MalI GATC 6 cut(s) 8, 69, 523, 781, 828, 874
MbiI CCGCTC 1 cut(s) 1265
MboI GATC 6 cut(s) 6, 67, 521, 779, 826, 872
MfeI CAATTG 1 cut(s) 1161
MflI RGATCY 1 cut(s) 521
MhlI GDGCHC 3 cut(s) 221, 300, 1221
MluCI AATT 5 cut(s) 249, 328, 431, 1161, 1257
MlyI GAGTC 2 cut(s) 178, 602
Mph1103I ATGCAT 2 cut(s) 100, 179
MroXI GAANNNNTTC 3 cut(s) 117, 240, 342
MseI TTAA 1 cut(s) 410
MspI CCGG 2 cut(s) 714, 1232
MspR9I CCNGG 3 cut(s) 599, 715, 906
MunI CAATTG 1 cut(s) 1161
Mva1269I GAATGC 1 cut(s) 233
MvaI CCWGG 2 cut(s) 599, 906
MvnI CGCG 1 cut(s) 35
MwoI GCNNNNNNNGC 4 cut(s) 272, 1121, 1225, 1259
NciI CCSGG 1 cut(s) 715
NdeII GATC 6 cut(s) 6, 67, 521, 779, 826, 872
NlaIII CATG 3 cut(s) 947, 1086, 1184
NlaIV GGNNCC 1 cut(s) 1170
NmuCI GTSAC 5 cut(s) 86, 605, 806, 908, 1020
NsiI ATGCAT 2 cut(s) 100, 179
NspI RCATGY 1 cut(s) 1184
PaqCI CACCTGC 1 cut(s) 504
PciSI GCTCTTC 1 cut(s) 47
PcsI WCGNNNNNNNCGW 1 cut(s) 395
PctI GAATGC 1 cut(s) 233
PdmI GAANNNNTTC 3 cut(s) 117, 240, 342
PfeI GAWTC 7 cut(s) 152, 403, 449, 916, 959, 1031, 1076
PkrI GCNGC 6 cut(s) 262, 265, 630, 638, 1252, 1264
PleI GAGTC 2 cut(s) 178, 602
PmaCI CACGTG 1 cut(s) 1216
PmlI CACGTG 1 cut(s) 1216
PpsI GAGTC 2 cut(s) 178, 602
Ppu21I YACGTR 1 cut(s) 1216
Psp124BI GAGCTC 1 cut(s) 300
Psp6I CCWGG 2 cut(s) 597, 904
PspCI CACGTG 1 cut(s) 1216
PspGI CCWGG 2 cut(s) 597, 904
PspN4I GGNNCC 1 cut(s) 1170
PstI CTGCAG 2 cut(s) 265, 633
PstNI CAGNNNCTG 1 cut(s) 472
PsuI RGATCY 1 cut(s) 521
RsaI GTAC 4 cut(s) 257, 709, 1212, 1238
RsaNI GTAC 4 cut(s) 256, 708, 1211, 1237
SacI GAGCTC 1 cut(s) 300
SapI GCTCTTC 1 cut(s) 47
SaqAI TTAA 1 cut(s) 410
SatI GCNGC 6 cut(s) 261, 264, 629, 637, 1251, 1263
Sau3AI GATC 6 cut(s) 6, 67, 521, 779, 826, 872
SchI GAGTC 2 cut(s) 178, 602
ScrFI CCNGG 3 cut(s) 599, 715, 906
SduI GDGCHC 3 cut(s) 221, 300, 1221
SexAI ACCWGGT 1 cut(s) 904
SfaNI GCATC 6 cut(s) 164, 186, 503, 729, 1057, 1139
SfcI CTRYAG 3 cut(s) 261, 629, 775
Sse9I AATT 5 cut(s) 249, 328, 431, 1161, 1257
SsiI CCGC 4 cut(s) 4, 35, 141, 1263
SspI AATATT 1 cut(s) 686
SspMI CTAG 4 cut(s) 122, 834, 939, 1055
SstI GAGCTC 1 cut(s) 300
StyD4I CCNGG 3 cut(s) 597, 713, 904
StyI CCWWGG 1 cut(s) 502
TaaI ACNGT 3 cut(s) 474, 620, 1020
TaiI ACGT 2 cut(s) 813, 1218
TaqI TCGA 7 cut(s) 9, 182, 336, 437, 813, 1034, 1255
TaqII GACCGA 1 cut(s) 234
TasI AATT 5 cut(s) 249, 328, 431, 1161, 1257
TatI WGTACW 2 cut(s) 707, 1210
TauI GCSGC 1 cut(s) 1265
TfiI GAWTC 7 cut(s) 152, 403, 449, 916, 959, 1031, 1076
Tru1I TTAA 1 cut(s) 410
Tru9I TTAA 1 cut(s) 410
TscAI CASTG 1 cut(s) 646
TseFI GTSAC 5 cut(s) 86, 605, 806, 908, 1020
TseI GCWGC 5 cut(s) 260, 263, 628, 636, 1250
Tsp45I GTSAC 5 cut(s) 86, 605, 806, 908, 1020
TspDTI ATGAA 5 cut(s) 165, 735, 1044, 1058, 1257
TspGWI ACGGA 2 cut(s) 281, 1107
TspRI CASTG 1 cut(s) 646
VneI GTGCAC 1 cut(s) 1217
XapI RAATTY 2 cut(s) 328, 431
XbaI TCTAGA 1 cut(s) 1054
XceI RCATGY 1 cut(s) 1184
XcmI CCANNNNNNNNNTGG 1 cut(s) 515
XmiI GTMKAC 1 cut(s) 476
XmnI GAANNNNTTC 3 cut(s) 117, 240, 342
XspI CTAG 4 cut(s) 122, 834, 939, 1055
Zsp2I ATGCAT 2 cut(s) 100, 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.