RchiOBHm_Chr2g0138021

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0003526
Physical Location & Seq
Reverse (-)
55821771 .. 55825183
3413 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ50873

Sequence Viewer

Length: 1287 bp
ATGTTTGGGTTCGGGAGGCCTAAATTTGTCACTAAATGCAAATCTGATTTAAAGGGCATGAAGGTGAGGCTTGAGGCGGTTAAGAAGAAGAGGAACTCTGTGCTCAAGTATTTGAAGAATGATGTTGCCGAGCTTCTCAAGAATGGCCTTGATATTAATGCATATGGAAGGGCTGAGGGAGTTATGGTTGAGCAAAATATGTCTGCCTGTTATGCACTGACTGAGAACTTTCTTGACTGTATCGCGAATCATCTTTCTCTCATGCAAAATCAGAGGGAATGTCCTGAAGAATGCAAGGAAGCTGTTGCATCTGTGATATATGGTGCAGCAAGATTTTCTGATTTGCCAGAACTGCGTGACCTCAGAAACATATTTACTGGAAAGTATGGAGATTCCCTACAATCTTTTGTAAATAAAGAGTTTGCGGATAAATTCAAGTCAAAGGCCCCTACAAAGGAGATGAAGGTACAATTAATGCATGACATAGCGAGAGAATACTCAATCGAGTGGGACTCCAAGGCTCTGGAACAAAAGCTGTTTACTCCACCTCCAACAAACAAGGGGCAGCCTACGTATGGGTCTCCGTGTGACAGTGATGATGATATGTACAGATCACAGAAGAAGGATAACGCTCTTCCGAAGAGAAAAAATAAGGGTGATGGAGACAAACGGAGCTATGTAACTGATGATACTGCATCAGTAGGAAGTGATGATGAAGCAACAGATATGTCCCATGATGACCGAATGACTGCTTCAAGTTCAGTAGGAAGTAGATCCGAGGATGAAGCAGAAAACAAGGTGCCCTCTTCCTATAAGTTCACTGCACCCCCTTACTACAAACCAAAGCCTCAGAAAAAAGAAAGTGTCTTTGAGGAACCCAGGAAGGTGAATGGTCATGCTGAACGAAGGTCAGTTAGGAGAACCTCGAAACCACCAGCAGGTGGTGACAATCATGGAAGTGATCCGAGGGATGAAGAAGAGAGGGTAATGGATGGGCTTTTGATGCATTACAGTCATAAACAGTCACCCTATGACGCAAGCAAGATGAAGGGAAATCTAAAACCTTGCTACAACCAGTCAGATGAAAATACTAATGAATCCACAAGGCACAGAAGCAGCAGGTCTGAAAGAGCAGCATCTCTGCCACCACCAGAGCAATCAACTTCACCTGAGCCAACAAAAAGGCATGCTCGAGGCAATTCCTTACAGCCAGATGCCCATGTGCACCCGAACCTACCGGAGTATGATGAATTGGCAGCCCGAATTGCAGCTCTTAGAGGAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

428

Amino Acids

48.16

Weight (kDa)

8.44

Isoelectric Point (pI)

62.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 14 - 169 3.5e-45 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 929
Acc36I ACCTGC 2 cut(s) 929, 1110
AccB1I GGYRCC 1 cut(s) 799
AccB7I CCANNNNNTGG 1 cut(s) 941
AccII CGCG 1 cut(s) 245
AciI CCGC 2 cut(s) 77, 425
AclWI GGATC 2 cut(s) 768, 956
AcsI RAATTY 2 cut(s) 23, 431
AcuI CTGAAG 1 cut(s) 306
AfaI GTAC 2 cut(s) 468, 608
AfiI CCNNNNNNNGG 4 cut(s) 454, 575, 938, 941
AgsI TTSAA 3 cut(s) 115, 436, 756
AjnI CCWGG 1 cut(s) 878
AluBI AGCT 5 cut(s) 133, 302, 535, 675, 1271
AluI AGCT 5 cut(s) 133, 302, 535, 675, 1271
Alw21I GWGCWC 2 cut(s) 105, 1227
Alw26I GTCTC 2 cut(s) 585, 657
Alw44I GTGCAC 1 cut(s) 1223
AlwI GGATC 2 cut(s) 768, 956
Ama87I CYCGRG 1 cut(s) 1191
AoxI GGCC 3 cut(s) 17, 145, 444
ApaLI GTGCAC 1 cut(s) 1223
ApeKI GCWGC 6 cut(s) 326, 565, 1116, 1133, 1256, 1268
ApoI RAATTY 2 cut(s) 23, 431
AseI ATTAAT 2 cut(s) 156, 473
AspS9I GGNCC 1 cut(s) 445
AsuHPI GGTGA 6 cut(s) 76, 668, 898, 956, 1017, 1158
AvaI CYCGRG 1 cut(s) 1191
BaeGI GKGCMC 2 cut(s) 804, 1227
BanI GGYRCC 1 cut(s) 799
Bbv12I GWGCWC 2 cut(s) 105, 1227
BbvCI CCTCAGC 1 cut(s) 174
BbvI GCAGC 6 cut(s) 338, 577, 1128, 1145, 1268, 1280
BccI CCATC 2 cut(s) 653, 986
BciT130I CCWGG 1 cut(s) 880
BcoDI GTCTC 2 cut(s) 585, 657
BfuAI ACCTGC 2 cut(s) 929, 1110
BisI GCNGC 6 cut(s) 327, 566, 1117, 1134, 1257, 1269
BlsI GCNGC 6 cut(s) 328, 567, 1118, 1135, 1258, 1270
Bme1390I CCNGG 1 cut(s) 880
BmeT110I CYCGRG 1 cut(s) 1191
BmgT120I GGNCC 1 cut(s) 445
BmiI GGNNCC 3 cut(s) 447, 801, 876
BmrFI CCNGG 1 cut(s) 880
BmsI GCATC 5 cut(s) 317, 704, 993, 1145, 1204
BplI GAGNNNNNCTC 2 cut(s) 497, 529
Bpu10I CCTNAGC 2 cut(s) 174, 1170
BpuEI CTTGAG 3 cut(s) 89, 92, 122
BsaAI YACGTR 1 cut(s) 573
BsaI GGTCTC 1 cut(s) 585
BsaJI CCNNGG 4 cut(s) 516, 777, 878, 965
BsaWI WCCGGW 1 cut(s) 1237
BsaXI ACNNNNNCTCC 4 cut(s) 171, 201, 532, 562
Bsc4I CCNNNNNNNGG 4 cut(s) 454, 575, 938, 941
Bse1I ACTGG 2 cut(s) 382, 1075
BseBI CCWGG 1 cut(s) 880
BseDI CCNNGG 4 cut(s) 516, 777, 878, 965
BseGI GGATG 3 cut(s) 787, 976, 997
BseLI CCNNNNNNNGG 4 cut(s) 454, 575, 938, 941
BseMII CTCAG 5 cut(s) 165, 213, 376, 863, 1161
BseNI ACTGG 2 cut(s) 382, 1075
BseSI GKGCMC 2 cut(s) 804, 1227
BseXI GCAGC 6 cut(s) 338, 577, 1128, 1145, 1268, 1280
BsgI GTGCAG 2 cut(s) 345, 807
Bsh1236I CGCG 1 cut(s) 245
BshFI GGCC 3 cut(s) 19, 147, 446
BshNI GGYRCC 1 cut(s) 799
BsiHKAI GWGCWC 2 cut(s) 105, 1227
BsiHKCI CYCGRG 1 cut(s) 1191
BsiSI CCGG 1 cut(s) 1238
BslFI GGGAC 2 cut(s) 524, 715
BslI CCNNNNNNNGG 4 cut(s) 454, 575, 938, 941
BsmAI GTCTC 2 cut(s) 585, 657
BsmFI GGGAC 2 cut(s) 524, 715
BsmI GAATGC 1 cut(s) 296
BsnI GGCC 3 cut(s) 19, 147, 446
Bso31I GGTCTC 1 cut(s) 585
BsoBI CYCGRG 1 cut(s) 1191
Bsp1286I GDGCHC 3 cut(s) 105, 804, 1227
Bsp1407I TGTACA 1 cut(s) 606
Bsp143I GATC 3 cut(s) 611, 773, 961
Bsp68I TCGCGA 1 cut(s) 245
BspACI CCGC 2 cut(s) 77, 425
BspANI GGCC 3 cut(s) 19, 147, 446
BspCNI CTCAG 5 cut(s) 166, 214, 375, 862, 1162
BspFNI CGCG 1 cut(s) 245
BspLI GGNNCC 3 cut(s) 447, 801, 876
BspMI ACCTGC 2 cut(s) 929, 1110
BspPI GGATC 2 cut(s) 768, 956
BspQI GCTCTTC 1 cut(s) 639
BspT107I GGYRCC 1 cut(s) 799
BspTNI GGTCTC 1 cut(s) 585
BsrGI TGTACA 1 cut(s) 606
BsrI ACTGG 2 cut(s) 382, 1075
BssECI CCNNGG 4 cut(s) 516, 777, 878, 965
BssMI GATC 3 cut(s) 611, 773, 961
BssT1I CCWWGG 1 cut(s) 516
Bst2UI CCWGG 1 cut(s) 880
Bst4CI ACNGT 4 cut(s) 239, 593, 1013, 1023
Bst6I CTCTTC 5 cut(s) 83, 635, 639, 811, 972
BstAUI TGTACA 1 cut(s) 606
BstBAI YACGTR 1 cut(s) 573
BstC8I GCNNGC 2 cut(s) 1039, 1188
BstDEI CTNAG 6 cut(s) 174, 222, 362, 849, 1170, 1274
BstF5I GGATG 3 cut(s) 787, 976, 997
BstFNI CGCG 1 cut(s) 245
BstKTI GATC 3 cut(s) 614, 776, 964
BstMAI GTCTC 2 cut(s) 585, 657
BstMBI GATC 3 cut(s) 611, 773, 961
BstMWI GCNNNNNNNGC 4 cut(s) 212, 352, 1003, 1265
BstNI CCWGG 1 cut(s) 880
BstNSI RCATGY 1 cut(s) 1190
BstSCI CCNGG 1 cut(s) 878
BstSLI GKGCMC 2 cut(s) 804, 1227
BstSNI TACGTA 1 cut(s) 573
BstUI CGCG 1 cut(s) 245
BstV1I GCAGC 6 cut(s) 338, 577, 1128, 1145, 1268, 1280
BstX2I RGATCY 1 cut(s) 773
BstXI CCANNNNNNTGG 1 cut(s) 523
BstYI RGATCY 1 cut(s) 773
BsuRI GGCC 3 cut(s) 19, 147, 446
BtsCI GGATG 3 cut(s) 787, 976, 997
BtsI GCAGTG 1 cut(s) 819
BtsIMutI CAGTG 3 cut(s) 215, 598, 819
BtuMI TCGCGA 1 cut(s) 245
BveI ACCTGC 2 cut(s) 929, 1110
Cac8I GCNNGC 2 cut(s) 1039, 1188
Cfr13I GGNCC 1 cut(s) 445
CseI GACGC 1 cut(s) 1043
Csp6I GTAC 2 cut(s) 467, 607
CviAII CATG 9 cut(s) 58, 262, 479, 734, 896, 953, 1187, 1220, 1284
CviQI GTAC 2 cut(s) 467, 607
DdeI CTNAG 6 cut(s) 174, 222, 362, 849, 1170, 1274
DpnI GATC 3 cut(s) 613, 775, 963
DpnII GATC 3 cut(s) 611, 773, 961
DraI TTTAAA 1 cut(s) 51
Eam1104I CTCTTC 5 cut(s) 83, 635, 639, 811, 972
EarI CTCTTC 5 cut(s) 83, 635, 639, 811, 972
Eco105I TACGTA 1 cut(s) 573
Eco130I CCWWGG 1 cut(s) 516
Eco147I AGGCCT 1 cut(s) 19
Eco31I GGTCTC 1 cut(s) 585
Eco57I CTGAAG 1 cut(s) 306
Eco88I CYCGRG 1 cut(s) 1191
EcoO109I RGGNCCY 1 cut(s) 445
EcoRII CCWGG 1 cut(s) 878
EcoT14I CCWWGG 1 cut(s) 516
EcoT22I ATGCAT 3 cut(s) 163, 480, 1008
ErhI CCWWGG 1 cut(s) 516
FaeI CATG 9 cut(s) 61, 265, 482, 737, 899, 956, 1190, 1223, 1287
FaqI GGGAC 2 cut(s) 524, 715
FatI CATG 9 cut(s) 57, 261, 478, 733, 895, 952, 1186, 1219, 1283
FauNDI CATATG 1 cut(s) 163
Fnu4HI GCNGC 6 cut(s) 327, 566, 1117, 1134, 1257, 1269
FokI GGATG 3 cut(s) 794, 983, 1004
Fsp4HI GCNGC 6 cut(s) 327, 566, 1117, 1134, 1257, 1269
GluI GCNGC 6 cut(s) 327, 566, 1117, 1134, 1257, 1269
HaeIII GGCC 3 cut(s) 19, 147, 446
HapII CCGG 1 cut(s) 1238
HgaI GACGC 1 cut(s) 1043
Hin1II CATG 9 cut(s) 61, 265, 482, 737, 899, 956, 1190, 1223, 1287
HinfI GANTC 4 cut(s) 247, 392, 512, 1097
HpaII CCGG 1 cut(s) 1238
HphI GGTGA 6 cut(s) 76, 668, 898, 956, 1017, 1158
Hpy166II GTNNAC 3 cut(s) 540, 819, 1225
Hpy188III TCNNGA 6 cut(s) 13, 139, 233, 244, 284, 524
Hpy8I GTNNAC 3 cut(s) 540, 819, 1225
HpyAV CCTTC 7 cut(s) 55, 162, 457, 616, 877, 900, 1042
HpyCH4III ACNGT 4 cut(s) 239, 593, 1013, 1023
HpyCH4IV ACGT 1 cut(s) 572
HpyF10VI GCNNNNNNNGC 4 cut(s) 212, 352, 1003, 1265
HpyF3I CTNAG 6 cut(s) 174, 222, 362, 849, 1170, 1274
HpySE526I ACGT 1 cut(s) 572
Hsp92II CATG 9 cut(s) 61, 265, 482, 737, 899, 956, 1190, 1223, 1287
Kzo9I GATC 3 cut(s) 611, 773, 961
LguI GCTCTTC 1 cut(s) 639
LmnI GCTCC 1 cut(s) 672
Lsp1109I GCAGC 6 cut(s) 338, 577, 1128, 1145, 1268, 1280
LweI GCATC 5 cut(s) 317, 704, 993, 1145, 1204
MaeII ACGT 1 cut(s) 572
MaeIII GTNAC 6 cut(s) 28, 356, 587, 679, 944, 1023
MalI GATC 3 cut(s) 613, 775, 963
MboI GATC 3 cut(s) 611, 773, 961
MflI RGATCY 1 cut(s) 773
MhlI GDGCHC 3 cut(s) 105, 804, 1227
MluCI AATT 6 cut(s) 23, 431, 470, 1198, 1250, 1263
MlyI GAGTC 1 cut(s) 506
MmeI TCCRAC 1 cut(s) 575
Mph1103I ATGCAT 3 cut(s) 163, 480, 1008
MseI TTAA 4 cut(s) 50, 81, 156, 473
MslI CAYNNNNRTG 2 cut(s) 62, 957
MspI CCGG 1 cut(s) 1238
MspR9I CCNGG 1 cut(s) 880
Mva1269I GAATGC 1 cut(s) 296
MvaI CCWGG 1 cut(s) 880
MvnI CGCG 1 cut(s) 245
MwoI GCNNNNNNNGC 4 cut(s) 212, 352, 1003, 1265
NdeI CATATG 1 cut(s) 163
NdeII GATC 3 cut(s) 611, 773, 961
NlaIII CATG 9 cut(s) 61, 265, 482, 737, 899, 956, 1190, 1223, 1287
NlaIV GGNNCC 3 cut(s) 447, 801, 876
NmeAIII GCCGAG 1 cut(s) 154
NmuCI GTSAC 5 cut(s) 28, 356, 587, 944, 1023
NruI TCGCGA 1 cut(s) 245
NsiI ATGCAT 3 cut(s) 163, 480, 1008
NspI RCATGY 1 cut(s) 1190
PaeI GCATGC 1 cut(s) 1190
PaeR7I CTCGAG 1 cut(s) 1191
PaqCI CACCTGC 1 cut(s) 929
PceI AGGCCT 1 cut(s) 19
PciSI GCTCTTC 1 cut(s) 639
PctI GAATGC 1 cut(s) 296
PfeI GAWTC 3 cut(s) 247, 392, 1097
PflMI CCANNNNNTGG 1 cut(s) 941
PkrI GCNGC 6 cut(s) 328, 567, 1118, 1135, 1258, 1270
PleI GAGTC 1 cut(s) 506
PpsI GAGTC 1 cut(s) 506
Ppu21I YACGTR 1 cut(s) 573
PshBI ATTAAT 2 cut(s) 156, 473
Psp6I CCWGG 1 cut(s) 878
PspGI CCWGG 1 cut(s) 878
PspN4I GGNNCC 3 cut(s) 447, 801, 876
PspPI GGNCC 1 cut(s) 445
PspXI VCTCGAGB 1 cut(s) 1191
PsuI RGATCY 1 cut(s) 773
RruI TCGCGA 1 cut(s) 245
RsaI GTAC 2 cut(s) 468, 608
RsaNI GTAC 2 cut(s) 467, 607
RseI CAYNNNNRTG 2 cut(s) 62, 957
SapI GCTCTTC 1 cut(s) 639
SaqAI TTAA 4 cut(s) 50, 81, 156, 473
SatI GCNGC 6 cut(s) 327, 566, 1117, 1134, 1257, 1269
Sau3AI GATC 3 cut(s) 611, 773, 961
Sau96I GGNCC 1 cut(s) 445
SchI GAGTC 1 cut(s) 506
ScrFI CCNGG 1 cut(s) 880
SduI GDGCHC 3 cut(s) 105, 804, 1227
SfaNI GCATC 5 cut(s) 317, 704, 993, 1145, 1204
Sfr274I CTCGAG 1 cut(s) 1191
SlaI CTCGAG 1 cut(s) 1191
SmiMI CAYNNNNRTG 2 cut(s) 62, 957
SmlI CTYRAG 4 cut(s) 71, 104, 137, 1191
SmoI CTYRAG 4 cut(s) 71, 104, 137, 1191
SnaBI TACGTA 1 cut(s) 573
SphI GCATGC 1 cut(s) 1190
Sse9I AATT 6 cut(s) 23, 431, 470, 1198, 1250, 1263
SseBI AGGCCT 1 cut(s) 19
SsiI CCGC 2 cut(s) 77, 425
StuI AGGCCT 1 cut(s) 19
StyD4I CCNGG 1 cut(s) 878
StyI CCWWGG 1 cut(s) 516
TaaI ACNGT 4 cut(s) 239, 593, 1013, 1023
TaiI ACGT 1 cut(s) 575
TaqI TCGA 3 cut(s) 504, 926, 1192
TaqII GACCGA 1 cut(s) 756
TasI AATT 6 cut(s) 23, 431, 470, 1198, 1250, 1263
TatI WGTACW 1 cut(s) 606
TfiI GAWTC 3 cut(s) 247, 392, 1097
Tru1I TTAA 4 cut(s) 50, 81, 156, 473
Tru9I TTAA 4 cut(s) 50, 81, 156, 473
TscAI CASTG 3 cut(s) 222, 598, 826
TseFI GTSAC 5 cut(s) 28, 356, 587, 944, 1023
TseI GCWGC 6 cut(s) 326, 565, 1116, 1133, 1256, 1268
Tsp45I GTSAC 5 cut(s) 28, 356, 587, 944, 1023
TspDTI ATGAA 9 cut(s) 74, 476, 729, 798, 987, 1061, 1098, 1110, 1263
TspGWI ACGGA 2 cut(s) 573, 685
TspRI CASTG 3 cut(s) 222, 598, 826
Van91I CCANNNNNTGG 1 cut(s) 941
VneI GTGCAC 1 cut(s) 1223
VspI ATTAAT 2 cut(s) 156, 473
XapI RAATTY 2 cut(s) 23, 431
XceI RCATGY 1 cut(s) 1190
XhoI CTCGAG 1 cut(s) 1191
Zsp2I ATGCAT 3 cut(s) 163, 480, 1008
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.