Rh2CG382300

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0003526
Physical Location & Seq
Reverse (-)
52148724 .. 52152826
4103 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG382300.1

Sequence Viewer

Length: 1140 bp
ATGCTCTTAACTTTTCATAAACAGGCTGAGGGAGTTCTGGTTGAGCAAAATATGTCTGCCTGTTATGCACTGACTGAGAACTTTCTTGACTGTATCGCGAATCATCTTTCTCTCATGCAAAATCAGAGGGAATGTCCTGAAGAATGCAAGGAAGCTGTTGCATCTGTGATATATGCTGCAGCAAGATTTTCTGATTTGCCAGAACTGCGTGACCTCAGAAACATATTTACTGGAAAGTATGGAGATTCCCTACAATCTTTTGTAAATAAAGAGTTTGCGGATAAATTCAAGTCAAAGGCCCCTACAAAGGAGATGAAGGTACAATTAATGCATGACATAGCGAGAGAATACTCAATCGAGTGGGACTCCAAGGCTCTGGAACAAAAGCTGTTTACTCCACCTCCAACAAACAAGGGGCAGCCTACGTATGGGTCTCCGTGTGATAGTGATGATGATATGTACAGATCACAGAAGAAGGATAACGCTCTTCCGAAGAGAAAAAATAAGGGTGATGGAGACAAACGGAGCTATGTAACTGATGATACTGCATCAGTAGGAAGTGATGATGAAGCAACAGATATGTCCCATGATGACCGAATGACTGCTTCAAGTTCAGTAGGAAGTAGATCCGAGGATGAAGCAGAAAACAAGGTGCCCTCTTCCTATAAGTTCACTGCACCCCCTTACTACAAACCAAAGCCTCAGAAAAAAGAAAGTGTCTTTGAGGAACCCAGGAAGGTGAATGGTCATGCTGAACGAAGGTCAGTTAGGAGAACCTCGAAACCACCAGCAGGTGGTGACAATCATGGAAGTGATCCGAGGGATGAAGAAGAGAGGGTAATGGATGGGCTTTTGATGCATTACAGTCATAAACAGTCACCCTATGACGCAAGCAAGATGAAGGGAAATCTAAAACCTTGCTACAACCAGTCAGATGAAAATACTAATGAATCCACAAGGCACAGAAGCAGCAGGTCTGAAAGAGCAGCATCTCTGCCACCACCAGAGCAATCAACTTCACCTGAGCCAACAAAAAGGCATGCTCGAGGCAATTCCTTACAACCAGATGCCCATGTGCACCCGAACCTACCGGAGTATGATGAATTGGCAGCCCGAATTGCAGCTCTTAGAGGAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

379

Amino Acids

42.65

Weight (kDa)

6.21

Isoelectric Point (pI)

72.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 8 - 120 5.2e-33 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 782
Acc36I ACCTGC 2 cut(s) 782, 963
AccB1I GGYRCC 1 cut(s) 652
AccB7I CCANNNNNTGG 1 cut(s) 794
AccII CGCG 1 cut(s) 98
AciI CCGC 1 cut(s) 278
AclWI GGATC 2 cut(s) 621, 809
AcsI RAATTY 1 cut(s) 284
AcuI CTGAAG 1 cut(s) 159
AfaI GTAC 2 cut(s) 321, 461
AfiI CCNNNNNNNGG 4 cut(s) 307, 428, 791, 794
AgsI TTSAA 2 cut(s) 289, 609
AjnI CCWGG 1 cut(s) 731
AluBI AGCT 4 cut(s) 155, 388, 528, 1124
AluI AGCT 4 cut(s) 155, 388, 528, 1124
Alw21I GWGCWC 1 cut(s) 1080
Alw26I GTCTC 2 cut(s) 438, 510
Alw44I GTGCAC 1 cut(s) 1076
AlwI GGATC 2 cut(s) 621, 809
Ama87I CYCGRG 1 cut(s) 1044
AoxI GGCC 1 cut(s) 297
ApaLI GTGCAC 1 cut(s) 1076
ApeKI GCWGC 7 cut(s) 176, 179, 418, 969, 986, 1109, 1121
ApoI RAATTY 1 cut(s) 284
AseI ATTAAT 1 cut(s) 326
AspS9I GGNCC 1 cut(s) 298
AsuHPI GGTGA 5 cut(s) 521, 751, 809, 870, 1011
AvaI CYCGRG 1 cut(s) 1044
BaeGI GKGCMC 2 cut(s) 657, 1080
BanI GGYRCC 1 cut(s) 652
Bbv12I GWGCWC 1 cut(s) 1080
BbvCI CCTCAGC 1 cut(s) 27
BbvI GCAGC 7 cut(s) 163, 191, 430, 981, 998, 1121, 1133
BccI CCATC 2 cut(s) 506, 839
BciT130I CCWGG 1 cut(s) 733
BcoDI GTCTC 2 cut(s) 438, 510
BfmI CTRYAG 1 cut(s) 177
BfuAI ACCTGC 2 cut(s) 782, 963
BisI GCNGC 7 cut(s) 177, 180, 419, 970, 987, 1110, 1122
BlsI GCNGC 7 cut(s) 178, 181, 420, 971, 988, 1111, 1123
Bme1390I CCNGG 1 cut(s) 733
BmeT110I CYCGRG 1 cut(s) 1044
BmgT120I GGNCC 1 cut(s) 298
BmiI GGNNCC 3 cut(s) 300, 654, 729
BmrFI CCNGG 1 cut(s) 733
BmsI GCATC 5 cut(s) 170, 557, 846, 998, 1057
BplI GAGNNNNNCTC 2 cut(s) 350, 382
Bpu10I CCTNAGC 2 cut(s) 27, 1023
BsaAI YACGTR 1 cut(s) 426
BsaI GGTCTC 1 cut(s) 438
BsaJI CCNNGG 4 cut(s) 369, 630, 731, 818
BsaWI WCCGGW 1 cut(s) 1090
BsaXI ACNNNNNCTCC 4 cut(s) 24, 54, 385, 415
Bsc4I CCNNNNNNNGG 4 cut(s) 307, 428, 791, 794
Bse1I ACTGG 2 cut(s) 235, 928
BseBI CCWGG 1 cut(s) 733
BseDI CCNNGG 4 cut(s) 369, 630, 731, 818
BseGI GGATG 3 cut(s) 640, 829, 850
BseLI CCNNNNNNNGG 4 cut(s) 307, 428, 791, 794
BseMII CTCAG 5 cut(s) 18, 66, 229, 716, 1014
BseNI ACTGG 2 cut(s) 235, 928
BseSI GKGCMC 2 cut(s) 657, 1080
BseXI GCAGC 7 cut(s) 163, 191, 430, 981, 998, 1121, 1133
BsgI GTGCAG 1 cut(s) 660
Bsh1236I CGCG 1 cut(s) 98
BshFI GGCC 1 cut(s) 299
BshNI GGYRCC 1 cut(s) 652
BsiHKAI GWGCWC 1 cut(s) 1080
BsiHKCI CYCGRG 1 cut(s) 1044
BsiSI CCGG 1 cut(s) 1091
BslFI GGGAC 2 cut(s) 377, 568
BslI CCNNNNNNNGG 4 cut(s) 307, 428, 791, 794
BsmAI GTCTC 2 cut(s) 438, 510
BsmFI GGGAC 2 cut(s) 377, 568
BsmI GAATGC 1 cut(s) 149
BsnI GGCC 1 cut(s) 299
Bso31I GGTCTC 1 cut(s) 438
BsoBI CYCGRG 1 cut(s) 1044
Bsp1286I GDGCHC 2 cut(s) 657, 1080
Bsp1407I TGTACA 1 cut(s) 459
Bsp143I GATC 3 cut(s) 464, 626, 814
Bsp68I TCGCGA 1 cut(s) 98
BspACI CCGC 1 cut(s) 278
BspANI GGCC 1 cut(s) 299
BspCNI CTCAG 5 cut(s) 19, 67, 228, 715, 1015
BspFNI CGCG 1 cut(s) 98
BspLI GGNNCC 3 cut(s) 300, 654, 729
BspMAI CTGCAG 1 cut(s) 181
BspMI ACCTGC 2 cut(s) 782, 963
BspPI GGATC 2 cut(s) 621, 809
BspQI GCTCTTC 1 cut(s) 492
BspT107I GGYRCC 1 cut(s) 652
BspTNI GGTCTC 1 cut(s) 438
BsrGI TGTACA 1 cut(s) 459
BsrI ACTGG 2 cut(s) 235, 928
BssECI CCNNGG 4 cut(s) 369, 630, 731, 818
BssMI GATC 3 cut(s) 464, 626, 814
BssT1I CCWWGG 1 cut(s) 369
Bst2UI CCWGG 1 cut(s) 733
Bst4CI ACNGT 3 cut(s) 92, 866, 876
Bst6I CTCTTC 4 cut(s) 488, 492, 664, 825
BstAUI TGTACA 1 cut(s) 459
BstBAI YACGTR 1 cut(s) 426
BstC8I GCNNGC 2 cut(s) 892, 1041
BstDEI CTNAG 6 cut(s) 27, 75, 215, 702, 1023, 1127
BstF5I GGATG 3 cut(s) 640, 829, 850
BstFNI CGCG 1 cut(s) 98
BstKTI GATC 3 cut(s) 467, 629, 817
BstMAI GTCTC 2 cut(s) 438, 510
BstMBI GATC 3 cut(s) 464, 626, 814
BstMWI GCNNNNNNNGC 4 cut(s) 65, 205, 856, 1118
BstNI CCWGG 1 cut(s) 733
BstNSI RCATGY 1 cut(s) 1043
BstSCI CCNGG 1 cut(s) 731
BstSFI CTRYAG 1 cut(s) 177
BstSLI GKGCMC 2 cut(s) 657, 1080
BstSNI TACGTA 1 cut(s) 426
BstUI CGCG 1 cut(s) 98
BstV1I GCAGC 7 cut(s) 163, 191, 430, 981, 998, 1121, 1133
BstX2I RGATCY 1 cut(s) 626
BstXI CCANNNNNNTGG 1 cut(s) 376
BstYI RGATCY 1 cut(s) 626
BsuRI GGCC 1 cut(s) 299
BtsCI GGATG 3 cut(s) 640, 829, 850
BtsI GCAGTG 1 cut(s) 672
BtsIMutI CAGTG 2 cut(s) 68, 672
BtuMI TCGCGA 1 cut(s) 98
BveI ACCTGC 2 cut(s) 782, 963
Cac8I GCNNGC 2 cut(s) 892, 1041
Cfr13I GGNCC 1 cut(s) 298
CseI GACGC 1 cut(s) 896
Csp6I GTAC 2 cut(s) 320, 460
CviAII CATG 8 cut(s) 115, 332, 587, 749, 806, 1040, 1073, 1137
CviQI GTAC 2 cut(s) 320, 460
DdeI CTNAG 6 cut(s) 27, 75, 215, 702, 1023, 1127
DpnI GATC 3 cut(s) 466, 628, 816
DpnII GATC 3 cut(s) 464, 626, 814
Eam1104I CTCTTC 4 cut(s) 488, 492, 664, 825
EarI CTCTTC 4 cut(s) 488, 492, 664, 825
Eco105I TACGTA 1 cut(s) 426
Eco130I CCWWGG 1 cut(s) 369
Eco31I GGTCTC 1 cut(s) 438
Eco57I CTGAAG 1 cut(s) 159
Eco88I CYCGRG 1 cut(s) 1044
EcoO109I RGGNCCY 1 cut(s) 298
EcoRII CCWGG 1 cut(s) 731
EcoT14I CCWWGG 1 cut(s) 369
EcoT22I ATGCAT 2 cut(s) 333, 861
ErhI CCWWGG 1 cut(s) 369
FaeI CATG 8 cut(s) 118, 335, 590, 752, 809, 1043, 1076, 1140
FaqI GGGAC 2 cut(s) 377, 568
FatI CATG 8 cut(s) 114, 331, 586, 748, 805, 1039, 1072, 1136
Fnu4HI GCNGC 7 cut(s) 177, 180, 419, 970, 987, 1110, 1122
FokI GGATG 3 cut(s) 647, 836, 857
Fsp4HI GCNGC 7 cut(s) 177, 180, 419, 970, 987, 1110, 1122
GluI GCNGC 7 cut(s) 177, 180, 419, 970, 987, 1110, 1122
HaeIII GGCC 1 cut(s) 299
HapII CCGG 1 cut(s) 1091
HgaI GACGC 1 cut(s) 896
Hin1II CATG 8 cut(s) 118, 335, 590, 752, 809, 1043, 1076, 1140
HinfI GANTC 4 cut(s) 100, 245, 365, 950
HpaII CCGG 1 cut(s) 1091
HphI GGTGA 5 cut(s) 521, 751, 809, 870, 1011
Hpy166II GTNNAC 3 cut(s) 393, 672, 1078
Hpy188I TCNGA 9 cut(s) 126, 193, 218, 492, 631, 705, 819, 934, 979
Hpy188III TCNNGA 4 cut(s) 86, 97, 137, 377
Hpy8I GTNNAC 3 cut(s) 393, 672, 1078
HpyAV CCTTC 5 cut(s) 310, 469, 730, 753, 895
HpyCH4III ACNGT 3 cut(s) 92, 866, 876
HpyCH4IV ACGT 1 cut(s) 425
HpyF10VI GCNNNNNNNGC 4 cut(s) 65, 205, 856, 1118
HpyF3I CTNAG 6 cut(s) 27, 75, 215, 702, 1023, 1127
HpySE526I ACGT 1 cut(s) 425
Hsp92II CATG 8 cut(s) 118, 335, 590, 752, 809, 1043, 1076, 1140
Kzo9I GATC 3 cut(s) 464, 626, 814
LguI GCTCTTC 1 cut(s) 492
LmnI GCTCC 1 cut(s) 525
Lsp1109I GCAGC 7 cut(s) 163, 191, 430, 981, 998, 1121, 1133
LweI GCATC 5 cut(s) 170, 557, 846, 998, 1057
MaeII ACGT 1 cut(s) 425
MaeIII GTNAC 4 cut(s) 209, 532, 797, 876
MalI GATC 3 cut(s) 466, 628, 816
MboI GATC 3 cut(s) 464, 626, 814
MboII GAAGA 7 cut(s) 152, 479, 484, 505, 651, 839, 842
MflI RGATCY 1 cut(s) 626
MhlI GDGCHC 2 cut(s) 657, 1080
MluCI AATT 5 cut(s) 284, 323, 1051, 1103, 1116
MlyI GAGTC 1 cut(s) 359
MmeI TCCRAC 1 cut(s) 428
Mph1103I ATGCAT 2 cut(s) 333, 861
MseI TTAA 2 cut(s) 8, 326
MslI CAYNNNNRTG 1 cut(s) 810
MspI CCGG 1 cut(s) 1091
MspR9I CCNGG 1 cut(s) 733
Mva1269I GAATGC 1 cut(s) 149
MvaI CCWGG 1 cut(s) 733
MvnI CGCG 1 cut(s) 98
MwoI GCNNNNNNNGC 4 cut(s) 65, 205, 856, 1118
NdeII GATC 3 cut(s) 464, 626, 814
NlaIII CATG 8 cut(s) 118, 335, 590, 752, 809, 1043, 1076, 1140
NlaIV GGNNCC 3 cut(s) 300, 654, 729
NmuCI GTSAC 3 cut(s) 209, 797, 876
NruI TCGCGA 1 cut(s) 98
NsiI ATGCAT 2 cut(s) 333, 861
NspI RCATGY 1 cut(s) 1043
PaeI GCATGC 1 cut(s) 1043
PaeR7I CTCGAG 1 cut(s) 1044
PaqCI CACCTGC 1 cut(s) 782
PciSI GCTCTTC 1 cut(s) 492
PctI GAATGC 1 cut(s) 149
PfeI GAWTC 3 cut(s) 100, 245, 950
PflMI CCANNNNNTGG 1 cut(s) 794
PkrI GCNGC 7 cut(s) 178, 181, 420, 971, 988, 1111, 1123
PleI GAGTC 1 cut(s) 359
PpsI GAGTC 1 cut(s) 359
Ppu21I YACGTR 1 cut(s) 426
PshBI ATTAAT 1 cut(s) 326
Psp6I CCWGG 1 cut(s) 731
PspGI CCWGG 1 cut(s) 731
PspN4I GGNNCC 3 cut(s) 300, 654, 729
PspPI GGNCC 1 cut(s) 298
PspXI VCTCGAGB 1 cut(s) 1044
PstI CTGCAG 1 cut(s) 181
PsuI RGATCY 1 cut(s) 626
RruI TCGCGA 1 cut(s) 98
RsaI GTAC 2 cut(s) 321, 461
RsaNI GTAC 2 cut(s) 320, 460
RseI CAYNNNNRTG 1 cut(s) 810
SapI GCTCTTC 1 cut(s) 492
SaqAI TTAA 2 cut(s) 8, 326
SatI GCNGC 7 cut(s) 177, 180, 419, 970, 987, 1110, 1122
Sau3AI GATC 3 cut(s) 464, 626, 814
Sau96I GGNCC 1 cut(s) 298
SchI GAGTC 1 cut(s) 359
ScrFI CCNGG 1 cut(s) 733
SduI GDGCHC 2 cut(s) 657, 1080
SfaNI GCATC 5 cut(s) 170, 557, 846, 998, 1057
SfcI CTRYAG 1 cut(s) 177
Sfr274I CTCGAG 1 cut(s) 1044
SlaI CTCGAG 1 cut(s) 1044
SmiMI CAYNNNNRTG 1 cut(s) 810
SmlI CTYRAG 1 cut(s) 1044
SmoI CTYRAG 1 cut(s) 1044
SnaBI TACGTA 1 cut(s) 426
SphI GCATGC 1 cut(s) 1043
Sse9I AATT 5 cut(s) 284, 323, 1051, 1103, 1116
SsiI CCGC 1 cut(s) 278
StyD4I CCNGG 1 cut(s) 731
StyI CCWWGG 1 cut(s) 369
TaaI ACNGT 3 cut(s) 92, 866, 876
TaiI ACGT 1 cut(s) 428
TaqI TCGA 3 cut(s) 357, 779, 1045
TaqII GACCGA 1 cut(s) 609
TasI AATT 5 cut(s) 284, 323, 1051, 1103, 1116
TatI WGTACW 1 cut(s) 459
TfiI GAWTC 3 cut(s) 100, 245, 950
Tru1I TTAA 2 cut(s) 8, 326
Tru9I TTAA 2 cut(s) 8, 326
TscAI CASTG 2 cut(s) 75, 679
TseFI GTSAC 3 cut(s) 209, 797, 876
TseI GCWGC 7 cut(s) 176, 179, 418, 969, 986, 1109, 1121
Tsp45I GTSAC 3 cut(s) 209, 797, 876
TspDTI ATGAA 9 cut(s) 5, 329, 582, 651, 840, 914, 951, 963, 1116
TspGWI ACGGA 2 cut(s) 426, 538
TspRI CASTG 2 cut(s) 75, 679
Van91I CCANNNNNTGG 1 cut(s) 794
VneI GTGCAC 1 cut(s) 1076
VspI ATTAAT 1 cut(s) 326
XapI RAATTY 1 cut(s) 284
XceI RCATGY 1 cut(s) 1043
XhoI CTCGAG 1 cut(s) 1044
Zsp2I ATGCAT 2 cut(s) 333, 861
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.