AT1G79910

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0003526
Physical Location & Seq
Forward (+)
30054552 .. 30057181
2630 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G79910.2

Sequence Viewer

Length: 1146 bp
ATGTTTGATGGGCTGTTCAAACCCAAGTTTTATACGAAATGCAAGTCGTTGGTCAAGATAACGAAGACAAGAGTAGACACGGTGAAGAGGAAGAAAAACTCGGTTTGCAAATATCTCAAGAACGACATTGTCGATCTTCTCAAGAACAGCCTCGATTACAATGCATATGGCAGGGCAGAAGGGCTTATAGAGGAGAAAAGGAGGCTGGCTTGTTATGAGTTTTTGGAGCAATTCTGCAACTGTGTTGCATCCAATGTCTCTCTATTACAGAAATCCATCCGGTGCCCTGATGAGTGCCGCGAGGCCATCTCCTCCCTTGTATATGCAGCCGCTAGAGTCTCTGAAGTCCCTGAGCTACGCGACCTCAGATCTTTATTTGCTGAGAGATACGGAAACACACTTGATCAGTTTGTGAACCCTGAGTTTGTAGAGAGATTCAAAGCAGAGCCGCCGTCAAAGGAAATGAAAGTGGAGCTGCTGCAGGAAATCGCCAGAGAGTACTCCATAAAATGGGATGCCAAGTCTTTAGAGCAGAGATTGTACACACCACCTCATCATCATCATGTCCATACGGAAACTGAAGAACCAAAAACAGAGAAGACCAACAGCGAACCCGAAAAGACCATGGAAAAGAGAAGCAGCCTTTCGTTCCATGGCAGAGAAGACTCGATGGATGACAGCAACAAGTCCATAATGAGTACAAGCGAAGAAGACTCCATGAGTACAAGCGGGAGTTGTATGACTGGCCCGGAGGATGATCCTGAAATGAAACCTTTCTACTACAGATTCATGACTCCTGCGCCTTACACCAAACCCAAGATCGAAAAACAGGAAAGTCTTCCGGAGAAGATGACTACATCTCATCTCATGGACACCGAATCGCCGACGGGCGGAAAACCAAAGCCGAGATCAGTGAGACGAAGATTCGCAAACCCGCCACCTGAAGCGGAAACGGAAGGGGAAGCATTGAAATTGAAAGTAGAGAGTAAGAACATGATGAGAGCATCAGAGTCGATGGGGAAAGCAAGCGGAGAGGGTTTTAGGAGAATGAGTCGTCGAACAGCCTCGTGGCAGCATAAGCCCTCCTCCTGCGTCCCCGATTTTGATGAGGTCGCCGCTCGCGTCGATGCCCTCTCCCGATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

381

Amino Acids

43.77

Weight (kDa)

8.23

Isoelectric Point (pI)

59.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 46 - 156 6e-35 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 282
AccB7I CCANNNNNTGG 1 cut(s) 510
AccBSI CCGCTC 1 cut(s) 1118
AccI GTMKAC 1 cut(s) 75
AccII CGCG 3 cut(s) 300, 360, 1122
AccIII TCCGGA 1 cut(s) 841
AciI CCGC 9 cut(s) 298, 330, 449, 729, 891, 935, 947, 1029, 1116
AclWI GGATC 1 cut(s) 752
AcuI CTGAAG 3 cut(s) 363, 600, 963
AfaI GTAC 4 cut(s) 500, 542, 700, 724
AfiI CCNNNNNNNGG 2 cut(s) 510, 890
AgsI TTSAA 4 cut(s) 19, 439, 970, 976
AluBI AGCT 2 cut(s) 355, 475
AluI AGCT 2 cut(s) 355, 475
Alw26I GTCTC 3 cut(s) 262, 343, 910
AlwI GGATC 1 cut(s) 752
Aor13HI TCCGGA 1 cut(s) 841
AoxI GGCC 2 cut(s) 303, 745
ApeKI GCWGC 5 cut(s) 326, 475, 478, 639, 1072
ArsI GACNNNNNNTTYG 2 cut(s) 29, 61
Asp700I GAANNNNTTC 2 cut(s) 773, 837
AspLEI GCGC 1 cut(s) 802
AspS9I GGNCC 1 cut(s) 746
AsuC2I CCSGG 1 cut(s) 749
AsuHPI GGTGA 1 cut(s) 94
BaeGI GKGCMC 1 cut(s) 287
BanI GGYRCC 1 cut(s) 282
BauI CACGAG 1 cut(s) 1066
BbsI GAAGAC 5 cut(s) 71, 605, 669, 717, 830
BbvI GCAGC 5 cut(s) 338, 462, 465, 651, 1084
BccI CCATC 5 cut(s) 2, 284, 314, 664, 1009
BceAI ACGGC 1 cut(s) 436
BcgI CGANNNNNNTGC 4 cut(s) 143, 177, 993, 1027
BclI TGATCA 1 cut(s) 403
BcnI CCSGG 1 cut(s) 749
BcoDI GTCTC 3 cut(s) 262, 343, 910
BfaI CTAG 1 cut(s) 333
BfmI CTRYAG 2 cut(s) 479, 781
BglII AGATCT 1 cut(s) 368
BisI GCNGC 9 cut(s) 298, 327, 330, 449, 476, 479, 640, 1073, 1116
BlsI GCNGC 9 cut(s) 299, 328, 331, 450, 477, 480, 641, 1074, 1117
BmcAI AGTACT 1 cut(s) 500
Bme1390I CCNGG 1 cut(s) 749
BmgT120I GGNCC 1 cut(s) 746
BmiI GGNNCC 1 cut(s) 284
BmrFI CCNGG 1 cut(s) 749
BmsI GCATC 4 cut(s) 257, 505, 1013, 1117
BpiI GAAGAC 5 cut(s) 71, 605, 669, 717, 830
BplI GAGNNNNNCTC 2 cut(s) 293, 325
Bpu10I CCTNAGC 1 cut(s) 351
BpuEI CTTGAG 2 cut(s) 101, 125
BpuMI CCSGG 1 cut(s) 749
BsaJI CCNNGG 2 cut(s) 624, 652
BsaWI WCCGGW 2 cut(s) 279, 841
BsaXI ACNNNNNCTCC 2 cut(s) 1036, 1066
Bsc4I CCNNNNNNNGG 2 cut(s) 510, 890
Bse1I ACTGG 1 cut(s) 748
BseAI TCCGGA 1 cut(s) 841
BseDI CCNNGG 2 cut(s) 624, 652
BseGI GGATG 5 cut(s) 248, 276, 520, 679, 760
BseLI CCNNNNNNNGG 2 cut(s) 510, 890
BseMII CTCAG 4 cut(s) 342, 372, 379, 411
BseNI ACTGG 1 cut(s) 748
BseRI GAGGAG 3 cut(s) 206, 301, 1075
BseSI GKGCMC 1 cut(s) 287
BseXI GCAGC 5 cut(s) 338, 462, 465, 651, 1084
Bsh1236I CGCG 3 cut(s) 300, 360, 1122
BshFI GGCC 2 cut(s) 305, 747
BshNI GGYRCC 1 cut(s) 282
BsiSI CCGG 3 cut(s) 280, 749, 842
BslFI GGGAC 2 cut(s) 332, 1079
BslI CCNNNNNNNGG 2 cut(s) 510, 890
BsmAI GTCTC 3 cut(s) 262, 343, 910
BsmBI CGTCTC 1 cut(s) 910
BsmFI GGGAC 2 cut(s) 332, 1079
BsnI GGCC 2 cut(s) 305, 747
Bsp1286I GDGCHC 1 cut(s) 287
Bsp13I TCCGGA 1 cut(s) 841
Bsp1407I TGTACA 1 cut(s) 540
Bsp143I GATC 6 cut(s) 133, 368, 403, 757, 819, 908
Bsp19I CCATGG 2 cut(s) 624, 652
BspACI CCGC 9 cut(s) 298, 330, 449, 729, 891, 935, 947, 1029, 1116
BspANI GGCC 2 cut(s) 305, 747
BspCNI CTCAG 4 cut(s) 343, 373, 378, 412
BspEI TCCGGA 1 cut(s) 841
BspFNI CGCG 3 cut(s) 300, 360, 1122
BspHI TCATGA 1 cut(s) 789
BspLI GGNNCC 1 cut(s) 284
BspMAI CTGCAG 1 cut(s) 483
BspPI GGATC 1 cut(s) 752
BspT107I GGYRCC 1 cut(s) 282
BsrBI CCGCTC 1 cut(s) 1118
BsrGI TGTACA 1 cut(s) 540
BsrI ACTGG 1 cut(s) 748
BssECI CCNNGG 2 cut(s) 624, 652
BssMI GATC 6 cut(s) 133, 368, 403, 757, 819, 908
BssSI CACGAG 1 cut(s) 1066
BssT1I CCWWGG 2 cut(s) 624, 652
Bst2BI CACGAG 1 cut(s) 1066
Bst4CI ACNGT 2 cut(s) 82, 242
Bst6I CTCTTC 1 cut(s) 80
BstAUI TGTACA 1 cut(s) 540
BstC8I GCNNGC 3 cut(s) 207, 1027, 1120
BstDEI CTNAG 4 cut(s) 351, 365, 381, 420
BstDSI CCRYGG 2 cut(s) 624, 652
BstF5I GGATG 5 cut(s) 248, 276, 520, 679, 760
BstFNI CGCG 3 cut(s) 300, 360, 1122
BstHHI GCGC 1 cut(s) 802
BstKTI GATC 6 cut(s) 136, 371, 406, 760, 822, 911
BstMAI GTCTC 3 cut(s) 262, 343, 910
BstMBI GATC 6 cut(s) 133, 368, 403, 757, 819, 908
BstMWI GCNNNNNNNGC 1 cut(s) 1078
BstSCI CCNGG 1 cut(s) 747
BstSFI CTRYAG 2 cut(s) 479, 781
BstSLI GKGCMC 1 cut(s) 287
BstUI CGCG 3 cut(s) 300, 360, 1122
BstV1I GCAGC 5 cut(s) 338, 462, 465, 651, 1084
BstV2I GAAGAC 5 cut(s) 71, 605, 669, 717, 830
BstX2I RGATCY 1 cut(s) 368
BstYI RGATCY 1 cut(s) 368
BsuRI GGCC 2 cut(s) 305, 747
BtgI CCRYGG 2 cut(s) 624, 652
BtsCI GGATG 5 cut(s) 248, 276, 520, 679, 760
BtsIMutI CAGTG 1 cut(s) 918
Cac8I GCNNGC 3 cut(s) 207, 1027, 1120
CciI TCATGA 1 cut(s) 789
CfoI GCGC 1 cut(s) 802
Cfr13I GGNCC 1 cut(s) 746
CseI GACGC 2 cut(s) 1081, 1111
Csp6I GTAC 4 cut(s) 499, 541, 699, 723
CviAII CATG 7 cut(s) 563, 625, 653, 718, 790, 868, 994
CviQI GTAC 4 cut(s) 499, 541, 699, 723
DdeI CTNAG 4 cut(s) 351, 365, 381, 420
DpnI GATC 6 cut(s) 135, 370, 405, 759, 821, 910
DpnII GATC 6 cut(s) 133, 368, 403, 757, 819, 908
Eam1104I CTCTTC 1 cut(s) 80
EarI CTCTTC 1 cut(s) 80
EciI GGCGGA 1 cut(s) 906
Eco130I CCWWGG 2 cut(s) 624, 652
Eco57I CTGAAG 3 cut(s) 363, 600, 963
EcoT14I CCWWGG 2 cut(s) 624, 652
EcoT22I ATGCAT 1 cut(s) 166
ErhI CCWWGG 2 cut(s) 624, 652
Esp3I CGTCTC 1 cut(s) 910
FaeI CATG 7 cut(s) 566, 628, 656, 721, 793, 871, 997
FaqI GGGAC 2 cut(s) 332, 1079
FatI CATG 7 cut(s) 562, 624, 652, 717, 789, 867, 993
FauI CCCGC 2 cut(s) 722, 942
FauNDI CATATG 1 cut(s) 166
FbaI TGATCA 1 cut(s) 403
FblI GTMKAC 1 cut(s) 75
Fnu4HI GCNGC 9 cut(s) 298, 327, 330, 449, 476, 479, 640, 1073, 1116
FokI GGATG 5 cut(s) 235, 263, 527, 686, 767
Fsp4HI GCNGC 9 cut(s) 298, 327, 330, 449, 476, 479, 640, 1073, 1116
FspBI CTAG 1 cut(s) 333
GlaI GCGC 1 cut(s) 801
GluI GCNGC 9 cut(s) 298, 327, 330, 449, 476, 479, 640, 1073, 1116
HaeIII GGCC 2 cut(s) 305, 747
HapII CCGG 3 cut(s) 280, 749, 842
HgaI GACGC 2 cut(s) 1081, 1111
HhaI GCGC 1 cut(s) 802
Hin1II CATG 7 cut(s) 566, 628, 656, 721, 793, 871, 997
Hin6I GCGC 1 cut(s) 800
HinP1I GCGC 1 cut(s) 800
HpaII CCGG 3 cut(s) 280, 749, 842
HphI GGTGA 1 cut(s) 94
Hpy166II GTNNAC 3 cut(s) 76, 415, 543
Hpy188I TCNGA 3 cut(s) 343, 368, 1009
Hpy188III TCNNGA 7 cut(s) 55, 118, 142, 761, 790, 842, 1137
Hpy8I GTNNAC 3 cut(s) 76, 415, 543
Hpy99I CGWCG 3 cut(s) 889, 1059, 1127
HpyAV CCTTC 2 cut(s) 173, 950
HpyCH4III ACNGT 2 cut(s) 82, 242
HpyCH4V TGCA 7 cut(s) 42, 108, 164, 237, 248, 326, 481
HpyF10VI GCNNNNNNNGC 1 cut(s) 1078
HpyF3I CTNAG 4 cut(s) 351, 365, 381, 420
Hsp92II CATG 7 cut(s) 566, 628, 656, 721, 793, 871, 997
HspAI GCGC 1 cut(s) 800
Kpn2I TCCGGA 1 cut(s) 841
Ksp22I TGATCA 1 cut(s) 403
Kzo9I GATC 6 cut(s) 133, 368, 403, 757, 819, 908
LmnI GCTCC 2 cut(s) 226, 472
Lsp1109I GCAGC 5 cut(s) 338, 462, 465, 651, 1084
LweI GCATC 4 cut(s) 257, 505, 1013, 1117
MaeI CTAG 1 cut(s) 333
MalI GATC 6 cut(s) 135, 370, 405, 759, 821, 910
MbiI CCGCTC 1 cut(s) 1118
MboI GATC 6 cut(s) 133, 368, 403, 757, 819, 908
MflI RGATCY 1 cut(s) 368
MhlI GDGCHC 1 cut(s) 287
MluCI AATT 2 cut(s) 230, 971
MlyI GAGTC 6 cut(s) 345, 659, 707, 787, 1019, 1060
Mph1103I ATGCAT 1 cut(s) 166
MroI TCCGGA 1 cut(s) 841
MroXI GAANNNNTTC 2 cut(s) 773, 837
MslI CAYNNNNRTG 1 cut(s) 561
MspI CCGG 3 cut(s) 280, 749, 842
MspR9I CCNGG 1 cut(s) 749
MvnI CGCG 3 cut(s) 300, 360, 1122
MwoI GCNNNNNNNGC 1 cut(s) 1078
NciI CCSGG 1 cut(s) 749
NcoI CCATGG 2 cut(s) 624, 652
NdeI CATATG 1 cut(s) 166
NdeII GATC 6 cut(s) 133, 368, 403, 757, 819, 908
NlaIII CATG 7 cut(s) 566, 628, 656, 721, 793, 871, 997
NlaIV GGNNCC 1 cut(s) 284
NmeAIII GCCGAG 1 cut(s) 930
NsiI ATGCAT 1 cut(s) 166
PagI TCATGA 1 cut(s) 789
PcsI WCGNNNNNNNCGW 2 cut(s) 129, 1119
PdmI GAANNNNTTC 2 cut(s) 773, 837
PfeI GAWTC 4 cut(s) 435, 786, 878, 924
PflFI GACNNNGTC 1 cut(s) 128
PflMI CCANNNNNTGG 1 cut(s) 510
PkrI GCNGC 9 cut(s) 299, 328, 331, 450, 477, 480, 641, 1074, 1117
PleI GAGTC 6 cut(s) 344, 659, 707, 787, 1018, 1059
PpsI GAGTC 6 cut(s) 344, 659, 707, 787, 1018, 1059
PspN4I GGNNCC 1 cut(s) 284
PspPI GGNCC 1 cut(s) 746
PstI CTGCAG 1 cut(s) 483
PsuI RGATCY 1 cut(s) 368
PsyI GACNNNGTC 1 cut(s) 128
RsaI GTAC 4 cut(s) 500, 542, 700, 724
RsaNI GTAC 4 cut(s) 499, 541, 699, 723
RseI CAYNNNNRTG 1 cut(s) 561
SatI GCNGC 9 cut(s) 298, 327, 330, 449, 476, 479, 640, 1073, 1116
Sau3AI GATC 6 cut(s) 133, 368, 403, 757, 819, 908
Sau96I GGNCC 1 cut(s) 746
ScaI AGTACT 1 cut(s) 500
SchI GAGTC 6 cut(s) 345, 659, 707, 787, 1019, 1060
ScrFI CCNGG 1 cut(s) 749
SduI GDGCHC 1 cut(s) 287
SetI ASST 7 cut(s) 357, 366, 477, 553, 775, 943, 1113
SfaNI GCATC 4 cut(s) 257, 505, 1013, 1117
SfcI CTRYAG 2 cut(s) 479, 781
SmiMI CAYNNNNRTG 1 cut(s) 561
SmlI CTYRAG 2 cut(s) 116, 140
SmoI CTYRAG 2 cut(s) 116, 140
Sse9I AATT 2 cut(s) 230, 971
SsiI CCGC 9 cut(s) 298, 330, 449, 729, 891, 935, 947, 1029, 1116
SspMI CTAG 1 cut(s) 333
StyD4I CCNGG 1 cut(s) 747
StyI CCWWGG 2 cut(s) 624, 652
TaaI ACNGT 2 cut(s) 82, 242
TaqI TCGA 7 cut(s) 132, 153, 668, 822, 1013, 1057, 1125
TasI AATT 2 cut(s) 230, 971
TatI WGTACW 4 cut(s) 498, 540, 698, 722
TauI GCSGC 4 cut(s) 300, 332, 451, 1118
TfiI GAWTC 4 cut(s) 435, 786, 878, 924
TscAI CASTG 1 cut(s) 918
TseI GCWGC 5 cut(s) 326, 475, 478, 639, 1072
TspDTI ATGAA 3 cut(s) 479, 778, 782
TspGWI ACGGA 3 cut(s) 405, 587, 968
TspRI CASTG 1 cut(s) 918
Tth111I GACNNNGTC 1 cut(s) 128
Van91I CCANNNNNTGG 1 cut(s) 510
XmiI GTMKAC 1 cut(s) 75
XmnI GAANNNNTTC 2 cut(s) 773, 837
XspI CTAG 1 cut(s) 333
ZrmI AGTACT 1 cut(s) 500
Zsp2I ATGCAT 1 cut(s) 166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.