Rh2AG115900

IST1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0003526
Physical Location & Seq
Forward (+)
10398971 .. 10401215
2245 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG115900.1

Sequence Viewer

Length: 669 bp
ATGACCGTCGCCACCGCAGCCTCAACCCAAACCAAGAAGCTCATGAAGCTCGGCCTCTCTCTTTTCCGATGGGGCTTCAACTCCTCCAAATGGGAGTGCCCGGCTGATCTCAAAGAAGGGATTGCTAGCTTAATCTTTGCAACACCAAGGTGCTCTGAGATTCCGGAACTGGTGGCACTAAGGAATATCTTTGAGAAGAAATATGGAAGAGATTTCGTAGCTGCAGCTACTGATTTGCGACCTGGATGTGGTGTGAATCGCATTCTCATTGACAAGCTGTCTGTTAGAACCCCTACGGGTGAAGTAAAGCTGAAAATCATGAAGGAAATAGCGAAGGAATACCAGGTTGATTGGGATACAACAGAATCTGAACAAGAACTTCTCAAACCTCCAGAAGAGACTCTAGATGGACCGCGTACTTTTGTTAGTGCTACCAGCTTGCCTGTGACAACCCAATCTTTTGAGTCTAACAAGTCAGAAACCAGGCTTTCTTCCGGTTCGTTGCGCAGAACAAGTGGTGGTGGACAGAGGGAAACCAGTTCTACGCACAGAATGAGTGATGGTGGAGAAAGGGAAACTAGTTCCTTCCGCAGAACAAGCGGTGGTGGAGAAAGAGAAACCAGTTCCTTCCGCAGAACAAGCGGTGGTGGAGAAAGAGAAACCAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

222

Amino Acids

24.52

Weight (kDa)

9.19

Isoelectric Point (pI)

47.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 29 - 117 1.4e-27 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 506
AccII CGCG 1 cut(s) 415
AccIII TCCGGA 1 cut(s) 163
AciI CCGC 6 cut(s) 15, 413, 589, 600, 631, 642
AfaI GTAC 1 cut(s) 418
AfiI CCNNNNNNNGG 2 cut(s) 90, 248
AgsI TTSAA 1 cut(s) 79
AhdI GACNNNNNGTC 1 cut(s) 277
AhlI ACTAGT 1 cut(s) 578
AjnI CCWGG 4 cut(s) 241, 342, 482, 662
AleI CACNNNNGTG 1 cut(s) 148
AluBI AGCT 8 cut(s) 40, 49, 129, 221, 227, 277, 310, 438
AluI AGCT 8 cut(s) 40, 49, 129, 221, 227, 277, 310, 438
Alw21I GWGCWC 1 cut(s) 155
Alw26I GTCTC 1 cut(s) 392
AlwNI CAGNNNCTG 2 cut(s) 230, 368
Aor13HI TCCGGA 1 cut(s) 163
AoxI GGCC 1 cut(s) 52
ApeKI GCWGC 3 cut(s) 17, 221, 224
AspLEI GCGC 1 cut(s) 507
AspS9I GGNCC 1 cut(s) 410
AsuC2I CCSGG 1 cut(s) 101
AsuHPI GGTGA 1 cut(s) 311
AsuNHI GCTAGC 1 cut(s) 125
AvaII GGWCC 1 cut(s) 410
BaeGI GKGCMC 1 cut(s) 101
Bbv12I GWGCWC 1 cut(s) 155
BbvI GCAGC 3 cut(s) 29, 208, 236
BccI CCATC 3 cut(s) 63, 401, 554
BciT130I CCWGG 4 cut(s) 243, 344, 484, 664
BciVI GTATCC 1 cut(s) 349
BcnI CCSGG 1 cut(s) 101
BcoDI GTCTC 1 cut(s) 392
BcuI ACTAGT 1 cut(s) 578
BfaI CTAG 3 cut(s) 126, 404, 579
BfmI CTRYAG 1 cut(s) 222
BfuI GTATCC 1 cut(s) 349
BisI GCNGC 3 cut(s) 18, 222, 225
BlsI GCNGC 3 cut(s) 19, 223, 226
Bme1390I CCNGG 5 cut(s) 101, 243, 344, 484, 664
Bme18I GGWCC 1 cut(s) 410
BmeRI GACNNNNNGTC 1 cut(s) 277
BmgT120I GGNCC 1 cut(s) 410
BmrFI CCNGG 5 cut(s) 101, 243, 344, 484, 664
BmtI GCTAGC 1 cut(s) 129
BpmI CTGGAG 1 cut(s) 375
BpuMI CCSGG 1 cut(s) 101
BsaJI CCNNGG 1 cut(s) 146
BsaWI WCCGGW 2 cut(s) 163, 494
Bsc4I CCNNNNNNNGG 2 cut(s) 90, 248
Bse1I ACTGG 3 cut(s) 174, 537, 621
BseAI TCCGGA 1 cut(s) 163
BseBI CCWGG 4 cut(s) 243, 344, 484, 664
BseDI CCNNGG 1 cut(s) 146
BseGI GGATG 1 cut(s) 251
BseLI CCNNNNNNNGG 2 cut(s) 90, 248
BseMII CTCAG 1 cut(s) 147
BseNI ACTGG 3 cut(s) 174, 537, 621
BseRI GAGGAG 1 cut(s) 73
BseSI GKGCMC 1 cut(s) 101
BseXI GCAGC 3 cut(s) 29, 208, 236
Bsh1236I CGCG 1 cut(s) 415
BshFI GGCC 1 cut(s) 54
BsiHKAI GWGCWC 1 cut(s) 155
BsiSI CCGG 3 cut(s) 101, 164, 495
BslI CCNNNNNNNGG 2 cut(s) 90, 248
BsmAI GTCTC 1 cut(s) 392
BsmI GAATGC 1 cut(s) 261
BsnI GGCC 1 cut(s) 54
Bsp1286I GDGCHC 2 cut(s) 101, 155
Bsp13I TCCGGA 1 cut(s) 163
Bsp143I GATC 1 cut(s) 106
BspACI CCGC 6 cut(s) 15, 413, 589, 600, 631, 642
BspANI GGCC 1 cut(s) 54
BspCNI CTCAG 1 cut(s) 148
BspEI TCCGGA 1 cut(s) 163
BspFNI CGCG 1 cut(s) 415
BspHI TCATGA 2 cut(s) 42, 318
BspMAI CTGCAG 1 cut(s) 226
BspOI GCTAGC 1 cut(s) 129
BsrI ACTGG 3 cut(s) 174, 537, 621
BssECI CCNNGG 1 cut(s) 146
BssMI GATC 1 cut(s) 106
BssT1I CCWWGG 1 cut(s) 146
Bst2UI CCWGG 4 cut(s) 243, 344, 484, 664
Bst4CI ACNGT 1 cut(s) 7
Bst6I CTCTTC 2 cut(s) 202, 390
BstC8I GCNNGC 2 cut(s) 127, 440
BstDEI CTNAG 2 cut(s) 156, 179
BstF5I GGATG 1 cut(s) 251
BstFNI CGCG 1 cut(s) 415
BstHHI GCGC 1 cut(s) 507
BstKTI GATC 1 cut(s) 109
BstMAI GTCTC 1 cut(s) 392
BstMBI GATC 1 cut(s) 106
BstMWI GCNNNNNNNGC 4 cut(s) 17, 46, 597, 639
BstNI CCWGG 4 cut(s) 243, 344, 484, 664
BstSCI CCNGG 5 cut(s) 99, 241, 342, 482, 662
BstSFI CTRYAG 1 cut(s) 222
BstSLI GKGCMC 1 cut(s) 101
BstUI CGCG 1 cut(s) 415
BstV1I GCAGC 3 cut(s) 29, 208, 236
BsuI GTATCC 1 cut(s) 349
BsuRI GGCC 1 cut(s) 54
BtsCI GGATG 1 cut(s) 251
Cac8I GCNNGC 2 cut(s) 127, 440
CaiI CAGNNNCTG 2 cut(s) 230, 368
CciI TCATGA 2 cut(s) 42, 318
CfoI GCGC 1 cut(s) 507
Cfr13I GGNCC 1 cut(s) 410
CsiI ACCWGGT 2 cut(s) 342, 662
Csp6I GTAC 1 cut(s) 417
CviAII CATG 2 cut(s) 43, 319
CviQI GTAC 1 cut(s) 417
DdeI CTNAG 2 cut(s) 156, 179
DpnI GATC 1 cut(s) 108
DpnII GATC 1 cut(s) 106
DriI GACNNNNNGTC 1 cut(s) 277
Eam1104I CTCTTC 2 cut(s) 202, 390
Eam1105I GACNNNNNGTC 1 cut(s) 277
EarI CTCTTC 2 cut(s) 202, 390
Eco130I CCWWGG 1 cut(s) 146
Eco47I GGWCC 1 cut(s) 410
EcoRII CCWGG 4 cut(s) 241, 342, 482, 662
EcoT14I CCWWGG 1 cut(s) 146
ErhI CCWWGG 1 cut(s) 146
FaeI CATG 2 cut(s) 46, 322
FaiI YATR 3 cut(s) 44, 204, 320
FatI CATG 2 cut(s) 42, 318
Fnu4HI GCNGC 3 cut(s) 18, 222, 225
FokI GGATG 1 cut(s) 258
Fsp4HI GCNGC 3 cut(s) 18, 222, 225
FspBI CTAG 3 cut(s) 126, 404, 579
FspI TGCGCA 1 cut(s) 506
GlaI GCGC 1 cut(s) 506
GluI GCNGC 3 cut(s) 18, 222, 225
GsuI CTGGAG 1 cut(s) 375
HaeIII GGCC 1 cut(s) 54
HapII CCGG 3 cut(s) 101, 164, 495
HhaI GCGC 1 cut(s) 507
Hin1II CATG 2 cut(s) 46, 322
Hin6I GCGC 1 cut(s) 505
HinP1I GCGC 1 cut(s) 505
HinfI GANTC 5 cut(s) 160, 256, 365, 400, 464
HpaII CCGG 3 cut(s) 101, 164, 495
HphI GGTGA 1 cut(s) 311
Hpy166II GTNNAC 1 cut(s) 524
Hpy188I TCNGA 4 cut(s) 68, 157, 370, 478
Hpy188III TCNNGA 5 cut(s) 43, 164, 319, 392, 404
Hpy8I GTNNAC 1 cut(s) 524
Hpy99I CGWCG 1 cut(s) 11
HpyAV CCTTC 5 cut(s) 110, 316, 328, 595, 637
HpyCH4III ACNGT 1 cut(s) 7
HpyCH4V TGCA 2 cut(s) 140, 224
HpyF10VI GCNNNNNNNGC 4 cut(s) 17, 46, 597, 639
HpyF3I CTNAG 2 cut(s) 156, 179
Hsp92II CATG 2 cut(s) 46, 322
HspAI GCGC 1 cut(s) 505
Kpn2I TCCGGA 1 cut(s) 163
Kzo9I GATC 1 cut(s) 106
Lsp1109I GCAGC 3 cut(s) 29, 208, 236
MabI ACCWGGT 2 cut(s) 342, 662
MaeI CTAG 3 cut(s) 126, 404, 579
MaeIII GTNAC 1 cut(s) 445
MalI GATC 1 cut(s) 108
MboI GATC 1 cut(s) 106
MboII GAAGA 4 cut(s) 208, 219, 407, 483
MhlI GDGCHC 2 cut(s) 101, 155
MlyI GAGTC 2 cut(s) 394, 473
MnlI CCTC 5 cut(s) 31, 65, 94, 399, 522
MroI TCCGGA 1 cut(s) 163
MseI TTAA 1 cut(s) 131
MslI CAYNNNNRTG 1 cut(s) 148
MspI CCGG 3 cut(s) 101, 164, 495
MspR9I CCNGG 5 cut(s) 101, 243, 344, 484, 664
Mva1269I GAATGC 1 cut(s) 261
MvaI CCWGG 4 cut(s) 243, 344, 484, 664
MvnI CGCG 1 cut(s) 415
MwoI GCNNNNNNNGC 4 cut(s) 17, 46, 597, 639
NciI CCSGG 1 cut(s) 101
NdeII GATC 1 cut(s) 106
NheI GCTAGC 1 cut(s) 125
NlaIII CATG 2 cut(s) 46, 322
NmeAIII GCCGAG 1 cut(s) 30
NmuCI GTSAC 1 cut(s) 445
NsbI TGCGCA 1 cut(s) 506
OliI CACNNNNGTG 1 cut(s) 148
PagI TCATGA 2 cut(s) 42, 318
PctI GAATGC 1 cut(s) 261
PfeI GAWTC 3 cut(s) 160, 256, 365
PkrI GCNGC 3 cut(s) 19, 223, 226
PleI GAGTC 2 cut(s) 394, 472
PpsI GAGTC 2 cut(s) 394, 472
Psp6I CCWGG 4 cut(s) 241, 342, 482, 662
PspGI CCWGG 4 cut(s) 241, 342, 482, 662
PspPI GGNCC 1 cut(s) 410
PstI CTGCAG 1 cut(s) 226
PstNI CAGNNNCTG 2 cut(s) 230, 368
RsaI GTAC 1 cut(s) 418
RsaNI GTAC 1 cut(s) 417
RseI CAYNNNNRTG 1 cut(s) 148
SaqAI TTAA 1 cut(s) 131
SatI GCNGC 3 cut(s) 18, 222, 225
Sau3AI GATC 1 cut(s) 106
Sau96I GGNCC 1 cut(s) 410
SchI GAGTC 2 cut(s) 394, 473
ScrFI CCNGG 5 cut(s) 101, 243, 344, 484, 664
SduI GDGCHC 2 cut(s) 101, 155
SexAI ACCWGGT 2 cut(s) 342, 662
SfcI CTRYAG 1 cut(s) 222
SinI GGWCC 1 cut(s) 410
SmiMI CAYNNNNRTG 1 cut(s) 148
SpeI ACTAGT 1 cut(s) 578
SsiI CCGC 6 cut(s) 15, 413, 589, 600, 631, 642
SspMI CTAG 3 cut(s) 126, 404, 579
StyD4I CCNGG 5 cut(s) 99, 241, 342, 482, 662
StyI CCWWGG 1 cut(s) 146
TaaI ACNGT 1 cut(s) 7
TfiI GAWTC 3 cut(s) 160, 256, 365
Tru1I TTAA 1 cut(s) 131
Tru9I TTAA 1 cut(s) 131
TseFI GTSAC 1 cut(s) 445
TseI GCWGC 3 cut(s) 17, 221, 224
Tsp45I GTSAC 1 cut(s) 445
TspDTI ATGAA 2 cut(s) 59, 335
VpaK11BI GGWCC 1 cut(s) 410
XbaI TCTAGA 1 cut(s) 403
XspI CTAG 3 cut(s) 126, 404, 579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.