Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr9

Orthogroup ID

OG0003526

Physical Location & Seq

Genomic Coordinates Forward (+)

11478542 .. 11481010

Gene Structure 2469 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom09g12950.6

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1347 bp

ATGCTGAGGGCGTTCCGAAAGCCGAAATTCGTCACCAAATGCAAATCGGATATAAAGACGACGAAGATGCGGATCGAGGCGATAAAGAAGAAGAGGAGCGCGGTGCAGAAGTTTTTGAAGAGCGACATAGCTGATCTTTTGAGGAGTGGCCGTGACATCAATGCATACGGCAGGGCTGAAGGGCTTCTAGTTGAGCAAAATATGTCTGCCTGTTACGAATCAACTGAGAGCTTTCTTGGATGCATCTCGACTCATCTTCCTCTCATACAAAGTCAGAGTGAGTGCCCTGAGGAATGCAAGGAAGCTGTTCGTTCAATTATGTACGCAGCAGCACGATTTGCAGATTTGCCAGAACTCCGCGACCTCAGAACTCTGTTTAGTGAAAAGTACGGAAATTCCCTCGAACCTTTTCTGAATAAAGAGTTTGTGGAGAGGTTGAAGTCAAAGCCTCCTACGAAGGAGATGAAGATTCAGTTAATGCACGACATAGCACAAGAATTTTCGATTGAATGGGATTCAAAAGCCTTGGAACAGAAACTGAATACTAATACTCCACTGGCATCTGACCAAGGCCAGCGCAGGTGCAGATCTCCAGTTGACAGCGATGACGATATGCACAAATCACATAAAAGGCAGGACAATGCATTCCCGAAGAGAAGAAAAGAGGAGGGTGACTCTTATGACAGATACAAGCTGCAGAGCAGCAGTGAAGACGAAACAACAACAGATGTATCCCAATCTGCCCCAAAATATTCTTCAAGCTCAATAGGAAGTACATCCGGGGATGAAGCGGATACCAGCAAGATGCCCTTCTACTCCAAGTTTATTCCACCACCTTACTACAAATCAAGACCTGAAAAAAAGGAAAGCAGCCACGTCGAAAAGGAAACAGATCACCCTAGCGATGTTTCAGTTGGCGAAGATAATACAAAACGAAGATCAGTAAGGAGGGGAAACTTGAAACCACCACCAGGTCATGAGAATCTTGGCAGGAATGATAAAACTAGACGAGGAGATGATAGTGATTCAATGGACGAAGAAGAGAGAATAATGGATGGGCTTTTGATGCATTACAGCAGGAAACAGTCACCCTATGAATTGAGCCAGATGAAGGGCAGTCTGGACGATGATGATGCAAGTGAATCCACATGGAACAGAAGCAGAAGATCTCAGCGAGAGGCTCCGTCAGCCAGAGCATCATCTCTCCCACCAGAGCCAACGAGTTCAATTGAGGCACCGAAAAAGCATGGCCGAGCATTTTCCTTCACACCAGATGTGCACGTGCACCCAAAGCTACCGGAGTACGATGAACTGGCAGCTCGAATTGCCGCTCTTAGAGGAATATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04144 map04144

Pfam Domains

Protein Families

Ist1

Protein Analysis

449

Amino Acids

50.99

Weight (kDa)

8.31

Isoelectric Point (pI)

78.38

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0003526)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G52315 AT1G52315 AT1G79910 AT1G79910 AT1G79910
fragaria_vesca	FvH4_6g31290 FvH4_6g31290
malus_domestica	MD09G1212300.v1.1 MD17G1194200.v1.1
prunus_persica	Prupe.3G070200_v2.0.a1 Prupe.3G070200_v2.0.a1 Prupe.3G070200_v2.0.a1 Prupe.3G070200_v2.0.a1 Prupe.3G070200_v2.0.a1
pyrus_communis	pycom09g12950
rosa_chinensis	RchiOBHm_Chr2g0138021 RchiOBHm_Chr6g0262501
rosa_laevigata	RLG00000014421 RLG00000019713
rosa_multiflora	Rmu_sc0000596.1_g000009 Rmu_sc0006745.1_g000003
rosa_roxburghii	Rroxscaffold_2G00106570 Rroxscaffold_7G00205210
rosa_rugosa	Rorug02G0349100
rosa_samantha	Rh2AG115900 Rh2AG395800 Rh2BG410900 Rh2CG382300 Rh2DG417600 Rh6BG112900 Rh6CG111200 Rh6DG100900
rosa_wichuraiana	Rw2G032320 Rw6G010270 Rw6G010710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	570
Acc36I	ACCTGC	1 cut(s)	570
AccB1I	GGYRCC	1 cut(s)	1234
AccBSI	CCGCTC	1 cut(s)	1331
AccII	CGCG	2 cut(s)	101, 360
AciI	CCGC	5 cut(s)	70, 101, 358, 791, 1329
AclWI	GGATC	1 cut(s)	80
AcoI	YGGCCR	2 cut(s)	148, 1249
AcsI	RAATTY	3 cut(s)	26, 394, 497
AcuI	CTGAAG	1 cut(s)	198
AcvI	CACGTG	1 cut(s)	1282
AfaI	GTAC	4 cut(s)	323, 389, 775, 1304
AfiI	CCNNNNNNNGG	2 cut(s)	971, 1111
AgsI	TTSAA	9 cut(s)	118, 315, 439, 509, 519, 759, 961, 1029, 1227
AjiI	CACGTC	1 cut(s)	877
AjnI	CCWGG	1 cut(s)	970
AjuI	GAANNNNNNNTTGG	2 cut(s)	739, 771
AluBI	AGCT	7 cut(s)	131, 231, 305, 694, 762, 1294, 1319
AluI	AGCT	7 cut(s)	131, 231, 305, 694, 762, 1294, 1319
Alw21I	GWGCWC	2 cut(s)	1281, 1287
Alw44I	GTGCAC	2 cut(s)	1277, 1283
AlwI	GGATC	1 cut(s)	80
AlwNI	CAGNNNCTG	1 cut(s)	538
AoxI	GGCC	3 cut(s)	148, 571, 1249
ApaLI	GTGCAC	2 cut(s)	1277, 1283
ApeKI	GCWGC	6 cut(s)	326, 329, 694, 702, 870, 1316
ApoI	RAATTY	3 cut(s)	26, 394, 497
Asp700I	GAANNNNTTC	3 cut(s)	183, 306, 408
AspLEI	GCGC	2 cut(s)	101, 579
AsuC2I	CCSGG	1 cut(s)	781
AsuHPI	GGTGA	4 cut(s)	25, 683, 887, 1080
AxyI	CCTNAGG	1 cut(s)	288
BaeGI	GKGCMC	3 cut(s)	287, 1281, 1287
BaeI	ACNNNNGTAYC	2 cut(s)	714, 747
BanI	GGYRCC	1 cut(s)	1234
BbrPI	CACGTG	1 cut(s)	1282
BbsI	GAAGAC	1 cut(s)	717
Bbv12I	GWGCWC	2 cut(s)	1281, 1287
BbvCI	CCTCAGC	1 cut(s)	5
BbvI	GCAGC	6 cut(s)	338, 341, 681, 714, 882, 1328
BccI	CCATC	1 cut(s)	1049
BceAI	ACGGC	2 cut(s)	135, 184
BcgI	CGANNNNNNTGC	6 cut(s)	49, 83, 859, 893, 1115, 1149
BciT130I	CCWGG	1 cut(s)	972
BciVI	GTATCC	2 cut(s)	742, 787
BcnI	CCSGG	1 cut(s)	781
BfaI	CTAG	3 cut(s)	188, 900, 1005
BfmI	CTRYAG	1 cut(s)	695
BfuAI	ACCTGC	1 cut(s)	570
BfuI	GTATCC	2 cut(s)	742, 787
BglII	AGATCT	2 cut(s)	587, 1166
BisI	GCNGC	7 cut(s)	327, 330, 695, 703, 871, 1317, 1329
BlsI	GCNGC	7 cut(s)	328, 331, 696, 704, 872, 1318, 1330
Bme1390I	CCNGG	2 cut(s)	781, 972
BmgBI	CACGTC	1 cut(s)	877
BmiI	GGNNCC	2 cut(s)	1182, 1236
BmrFI	CCNGG	2 cut(s)	781, 972
BmsI	GCATC	8 cut(s)	57, 230, 252, 569, 795, 1056, 1123, 1205
BpiI	GAAGAC	1 cut(s)	717
BplI	GAGNNNNNCTC	2 cut(s)	659, 691
BpmI	CTGGAG	1 cut(s)	576
Bpu10I	CCTNAGC	1 cut(s)	5
BpuMI	CCSGG	1 cut(s)	781
BsaAI	YACGTR	1 cut(s)	1282
BsaBI	GATNNNNATC	1 cut(s)	71
BsaJI	CCNNGG	3 cut(s)	525, 568, 780
BsaWI	WCCGGW	1 cut(s)	1297
BsaXI	ACNNNNNCTCC	4 cut(s)	136, 166, 535, 565
Bsc4I	CCNNNNNNNGG	2 cut(s)	971, 1111
Bse1I	ACTGG	3 cut(s)	561, 593, 1317
Bse21I	CCTNAGG	1 cut(s)	288
Bse8I	GATNNNNATC	1 cut(s)	71
BseBI	CCWGG	1 cut(s)	972
BseDI	CCNNGG	3 cut(s)	525, 568, 780
BseGI	GGATG	4 cut(s)	245, 776, 790, 1060
BseJI	GATNNNNATC	1 cut(s)	71
BseLI	CCNNNNNNNGG	2 cut(s)	971, 1111
BseMII	CTCAG	4 cut(s)	216, 279, 379, 1184
BseNI	ACTGG	3 cut(s)	561, 593, 1317
BseRI	GAGGAG	4 cut(s)	109, 157, 680, 1026
BseSI	GKGCMC	3 cut(s)	287, 1281, 1287
BseXI	GCAGC	6 cut(s)	338, 341, 681, 714, 882, 1328
BsgI	GTGCAG	2 cut(s)	125, 604
Bsh1236I	CGCG	2 cut(s)	101, 360
BshFI	GGCC	3 cut(s)	150, 573, 1251
BshNI	GGYRCC	1 cut(s)	1234
BsiHKAI	GWGCWC	2 cut(s)	1281, 1287
BsiSI	CCGG	2 cut(s)	780, 1298
BslI	CCNNNNNNNGG	2 cut(s)	971, 1111
BsmI	GAATGC	2 cut(s)	299, 644
BsnI	GGCC	3 cut(s)	150, 573, 1251
Bsp1286I	GDGCHC	3 cut(s)	287, 1281, 1287
Bsp143I	GATC	6 cut(s)	72, 133, 587, 892, 938, 1166
BspACI	CCGC	5 cut(s)	70, 101, 358, 791, 1329
BspANI	GGCC	3 cut(s)	150, 573, 1251
BspCNI	CTCAG	4 cut(s)	217, 280, 378, 1183
BspFNI	CGCG	2 cut(s)	101, 360
BspHI	TCATGA	1 cut(s)	976
BspLI	GGNNCC	2 cut(s)	1182, 1236
BspMAI	CTGCAG	1 cut(s)	699
BspMI	ACCTGC	1 cut(s)	570
BspPI	GGATC	1 cut(s)	80
BspQI	GCTCTTC	1 cut(s)	113
BspT107I	GGYRCC	1 cut(s)	1234
BsrBI	CCGCTC	1 cut(s)	1331
BsrI	ACTGG	3 cut(s)	561, 593, 1317
BssECI	CCNNGG	3 cut(s)	525, 568, 780
BssMI	GATC	6 cut(s)	72, 133, 587, 892, 938, 1166
BssT1I	CCWWGG	2 cut(s)	525, 568
Bst2UI	CCWGG	1 cut(s)	972
Bst4CI	ACNGT	1 cut(s)	1086
Bst6I	CTCTTC	4 cut(s)	86, 113, 647, 1035
BstAPI	GCANNNNNTGC	1 cut(s)	338
BstBAI	YACGTR	1 cut(s)	1282
BstC8I	GCNNGC	1 cut(s)	575
BstDEI	CTNAG	6 cut(s)	5, 225, 288, 365, 1170, 1334
BstF5I	GGATG	4 cut(s)	245, 776, 790, 1060
BstFNI	CGCG	2 cut(s)	101, 360
BstHHI	GCGC	2 cut(s)	101, 579
BstKTI	GATC	6 cut(s)	75, 136, 590, 895, 941, 1169
BstMBI	GATC	6 cut(s)	72, 133, 587, 892, 938, 1166
BstMWI	GCNNNNNNNGC	5 cut(s)	338, 1066, 1187, 1291, 1325
BstNI	CCWGG	1 cut(s)	972
BstSCI	CCNGG	2 cut(s)	779, 970
BstSFI	CTRYAG	1 cut(s)	695
BstSLI	GKGCMC	3 cut(s)	287, 1281, 1287
BstUI	CGCG	2 cut(s)	101, 360
BstV1I	GCAGC	6 cut(s)	338, 341, 681, 714, 882, 1328
BstV2I	GAAGAC	1 cut(s)	717
BstX2I	RGATCY	2 cut(s)	587, 1166
BstYI	RGATCY	2 cut(s)	587, 1166
Bsu36I	CCTNAGG	1 cut(s)	288
BsuI	GTATCC	2 cut(s)	742, 787
BsuRI	GGCC	3 cut(s)	150, 573, 1251
BtgZI	GCGATG	2 cut(s)	618, 918
BtrI	CACGTC	1 cut(s)	877
BtsCI	GGATG	4 cut(s)	245, 776, 790, 1060
BtsI	GCAGTG	1 cut(s)	712
BtsIMutI	CAGTG	2 cut(s)	554, 712
BveI	ACCTGC	1 cut(s)	570
Cac8I	GCNNGC	1 cut(s)	575
CaiI	CAGNNNCTG	1 cut(s)	538
CciI	TCATGA	1 cut(s)	976
CfoI	GCGC	2 cut(s)	101, 579
CsiI	ACCWGGT	1 cut(s)	970
Csp6I	GTAC	4 cut(s)	322, 388, 774, 1303
CviAII	CATG	3 cut(s)	977, 1149, 1247
CviQI	GTAC	4 cut(s)	322, 388, 774, 1303
DdeI	CTNAG	6 cut(s)	5, 225, 288, 365, 1170, 1334
DpnI	GATC	6 cut(s)	74, 135, 589, 894, 940, 1168
DpnII	GATC	6 cut(s)	72, 133, 587, 892, 938, 1166
EaeI	YGGCCR	2 cut(s)	148, 1249
Eam1104I	CTCTTC	4 cut(s)	86, 113, 647, 1035
EarI	CTCTTC	4 cut(s)	86, 113, 647, 1035
Eco130I	CCWWGG	2 cut(s)	525, 568
Eco57I	CTGAAG	1 cut(s)	198
Eco72I	CACGTG	1 cut(s)	1282
Eco81I	CCTNAGG	1 cut(s)	288
EcoRII	CCWGG	1 cut(s)	970
EcoT14I	CCWWGG	2 cut(s)	525, 568
EcoT22I	ATGCAT	4 cut(s)	166, 245, 646, 1071
ErhI	CCWWGG	2 cut(s)	525, 568
FaeI	CATG	3 cut(s)	980, 1152, 1250
FalI	AAGNNNNNCTT	2 cut(s)	794, 826
FatI	CATG	3 cut(s)	976, 1148, 1246
Fnu4HI	GCNGC	7 cut(s)	327, 330, 695, 703, 871, 1317, 1329
FokI	GGATG	4 cut(s)	252, 763, 797, 1067
Fsp4HI	GCNGC	7 cut(s)	327, 330, 695, 703, 871, 1317, 1329
FspBI	CTAG	3 cut(s)	188, 900, 1005
GlaI	GCGC	2 cut(s)	100, 578
GluI	GCNGC	7 cut(s)	327, 330, 695, 703, 871, 1317, 1329
GsuI	CTGGAG	1 cut(s)	576
HaeIII	GGCC	3 cut(s)	150, 573, 1251
HapII	CCGG	2 cut(s)	780, 1298
HhaI	GCGC	2 cut(s)	101, 579
Hin1II	CATG	3 cut(s)	980, 1152, 1250
Hin6I	GCGC	2 cut(s)	99, 577
HinP1I	GCGC	2 cut(s)	99, 577
HincII	GTYRAC	1 cut(s)	598
HindII	GTYRAC	1 cut(s)	598
HinfI	GANTC	8 cut(s)	218, 250, 469, 515, 674, 982, 1025, 1142
HpaII	CCGG	2 cut(s)	780, 1298
HphI	GGTGA	4 cut(s)	25, 683, 887, 1080
Hpy166II	GTNNAC	3 cut(s)	598, 1279, 1285
Hpy188I	TCNGA	6 cut(s)	17, 49, 276, 368, 414, 565
Hpy188III	TCNNGA	5 cut(s)	247, 649, 849, 977, 1121
Hpy8I	GTNNAC	3 cut(s)	598, 1279, 1285
Hpy99I	CGWCG	2 cut(s)	64, 881
HpyAV	CCTTC	5 cut(s)	173, 451, 820, 1105, 1273
HpyCH4III	ACNGT	1 cut(s)	1086
HpyCH4IV	ACGT	2 cut(s)	876, 1281
HpyF10VI	GCNNNNNNNGC	5 cut(s)	338, 1066, 1187, 1291, 1325
HpyF3I	CTNAG	6 cut(s)	5, 225, 288, 365, 1170, 1334
HpySE526I	ACGT	2 cut(s)	876, 1281
Hsp92II	CATG	3 cut(s)	980, 1152, 1250
HspAI	GCGC	2 cut(s)	99, 577
Kzo9I	GATC	6 cut(s)	72, 133, 587, 892, 938, 1166
LguI	GCTCTTC	1 cut(s)	113
LmnI	GCTCC	2 cut(s)	96, 1186
Lsp1109I	GCAGC	6 cut(s)	338, 341, 681, 714, 882, 1328
LweI	GCATC	8 cut(s)	57, 230, 252, 569, 795, 1056, 1123, 1205
MabI	ACCWGGT	1 cut(s)	970
MaeI	CTAG	3 cut(s)	188, 900, 1005
MaeII	ACGT	2 cut(s)	876, 1281
MaeIII	GTNAC	5 cut(s)	31, 152, 212, 671, 1086
MalI	GATC	6 cut(s)	74, 135, 589, 894, 940, 1168
MbiI	CCGCTC	1 cut(s)	1331
MboI	GATC	6 cut(s)	72, 133, 587, 892, 938, 1166
MfeI	CAATTG	1 cut(s)	1227
MflI	RGATCY	2 cut(s)	587, 1166
MhlI	GDGCHC	3 cut(s)	287, 1281, 1287
MluCI	AATT	7 cut(s)	26, 315, 394, 497, 1097, 1227, 1323
MlyI	GAGTC	2 cut(s)	244, 668
Mph1103I	ATGCAT	4 cut(s)	166, 245, 646, 1071
MroXI	GAANNNNTTC	3 cut(s)	183, 306, 408
MseI	TTAA	1 cut(s)	476
MspI	CCGG	2 cut(s)	780, 1298
MspR9I	CCNGG	2 cut(s)	781, 972
MunI	CAATTG	1 cut(s)	1227
Mva1269I	GAATGC	2 cut(s)	299, 644
MvaI	CCWGG	1 cut(s)	972
MvnI	CGCG	2 cut(s)	101, 360
MwoI	GCNNNNNNNGC	5 cut(s)	338, 1066, 1187, 1291, 1325
NciI	CCSGG	1 cut(s)	781
NdeII	GATC	6 cut(s)	72, 133, 587, 892, 938, 1166
NlaIII	CATG	3 cut(s)	980, 1152, 1250
NlaIV	GGNNCC	2 cut(s)	1182, 1236
NmeAIII	GCCGAG	1 cut(s)	1277
NmuCI	GTSAC	4 cut(s)	31, 152, 671, 1086
NsiI	ATGCAT	4 cut(s)	166, 245, 646, 1071
PagI	TCATGA	1 cut(s)	976
PaqCI	CACCTGC	1 cut(s)	570
PciSI	GCTCTTC	1 cut(s)	113
PctI	GAATGC	2 cut(s)	299, 644
PdmI	GAANNNNTTC	3 cut(s)	183, 306, 408
PfeI	GAWTC	6 cut(s)	218, 469, 515, 982, 1025, 1142
PkrI	GCNGC	7 cut(s)	328, 331, 696, 704, 872, 1318, 1330
PleI	GAGTC	2 cut(s)	244, 668
PmaCI	CACGTG	1 cut(s)	1282
PmlI	CACGTG	1 cut(s)	1282
PpsI	GAGTC	2 cut(s)	244, 668
Ppu21I	YACGTR	1 cut(s)	1282
Psp6I	CCWGG	1 cut(s)	970
PspCI	CACGTG	1 cut(s)	1282
PspGI	CCWGG	1 cut(s)	970
PspN4I	GGNNCC	2 cut(s)	1182, 1236
PstI	CTGCAG	1 cut(s)	699
PstNI	CAGNNNCTG	1 cut(s)	538
PsuI	RGATCY	2 cut(s)	587, 1166
RsaI	GTAC	4 cut(s)	323, 389, 775, 1304
RsaNI	GTAC	4 cut(s)	322, 388, 774, 1303
SapI	GCTCTTC	1 cut(s)	113
SaqAI	TTAA	1 cut(s)	476
SatI	GCNGC	7 cut(s)	327, 330, 695, 703, 871, 1317, 1329
Sau3AI	GATC	6 cut(s)	72, 133, 587, 892, 938, 1166
SchI	GAGTC	2 cut(s)	244, 668
ScrFI	CCNGG	2 cut(s)	781, 972
SduI	GDGCHC	3 cut(s)	287, 1281, 1287
SexAI	ACCWGGT	1 cut(s)	970
SfaNI	GCATC	8 cut(s)	57, 230, 252, 569, 795, 1056, 1123, 1205
SfcI	CTRYAG	1 cut(s)	695
Sse9I	AATT	7 cut(s)	26, 315, 394, 497, 1097, 1227, 1323
SsiI	CCGC	5 cut(s)	70, 101, 358, 791, 1329
SspI	AATATT	1 cut(s)	752
SspMI	CTAG	3 cut(s)	188, 900, 1005
StyD4I	CCNGG	2 cut(s)	779, 970
StyI	CCWWGG	2 cut(s)	525, 568
TaaI	ACNGT	1 cut(s)	1086
TaiI	ACGT	2 cut(s)	879, 1284
TaqI	TCGA	6 cut(s)	75, 248, 402, 503, 879, 1321
TasI	AATT	7 cut(s)	26, 315, 394, 497, 1097, 1227, 1323
TatI	WGTACW	1 cut(s)	773
TauI	GCSGC	1 cut(s)	1331
TfiI	GAWTC	6 cut(s)	218, 469, 515, 982, 1025, 1142
Tru1I	TTAA	1 cut(s)	476
Tru9I	TTAA	1 cut(s)	476
TscAI	CASTG	2 cut(s)	561, 712
TseFI	GTSAC	4 cut(s)	31, 152, 671, 1086
TseI	GCWGC	6 cut(s)	326, 329, 694, 702, 870, 1316
Tsp45I	GTSAC	4 cut(s)	31, 152, 671, 1086
TspDTI	ATGAA	5 cut(s)	479, 801, 1110, 1124, 1323
TspGWI	ACGGA	2 cut(s)	405, 1173
TspRI	CASTG	2 cut(s)	561, 712
VneI	GTGCAC	2 cut(s)	1277, 1283
XapI	RAATTY	3 cut(s)	26, 394, 497
XmnI	GAANNNNTTC	3 cut(s)	183, 306, 408
XspI	CTAG	3 cut(s)	188, 900, 1005
Zsp2I	ATGCAT	4 cut(s)	166, 245, 646, 1071

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.