Rh2AG395800

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0003526
Physical Location & Seq
Reverse (-)
59174952 .. 59178363
3412 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG395800.1

Sequence Viewer

Length: 1071 bp
ATGCAGTTTGTTCAACTTTTAGTTTCTCCTGCTCTTATTTGTTTTATCTGGTTTTATAGGGAATGTCCTGAAGAATGCAAGGAAGCTGTTGCATCTGTGATATATGCTGCAGCAAGATTTTCTGATTTGCCAGAACTGCGTGACCTCAGAAACATATTTACTGGAAAGTATGGAGATTCCCTACAATCTTTTGTAAATAAAGAGTTTGCGGATAAATTCAAGTCAAAGGCCCCTACAAAGGAGATGAAGGTACAATTAATGCATGACATAGCGAGAGAATACTCAATCGAGTGGGACTCCAAGGCTCTGGAACAAAAGCTGTTTACTCCACCTCCAACAAACAAGGGGCAGCCTACGTATGGGTCTCCGTGTGATAGTGATGATGATATGTACAGATCACAGAAGAAGGATAACGCTCTTCCGAAGAGAAAAAATAAGGGTGATGGAGACAAACGGAGCTATGTAACTGATGATACTGCATCAGTAGGAAGTGATGATGAAGCAACAGATATGTCCCATGATGACCGAATGACTGCTTCAAGTTCAGTAGGAAGTAGATCCGAGGATGAAGCAGAAAACAAGGTGCCCTCTTCCTATAAGTTCACTGCACCCCCTTACTACAAACCAAAGCCTCAGAAAAAAGAAAGTGTCTTTGAGGAACCCAGGAAGGTGAATGGTCATGCTGAACGAAGGTCAGTTAGGAGAACCTCGAAACCACCAGCAGGTGGTGACAATCATGGAAGTGATCCGAGGGATGAAGAAGAGAGGGTAATGGATGGGCTTTTGATGCATTACAGTCATAAACAGTCACCCTATGACGCAAGCAAGATGAAGGGAAATCTAAAACCTTGCTACAACCAGTCAGATGAAAATACTAATGAATCCACAAGGCACAGAAGCAGCAGGTCTGAAAGAGCAGCATCTCTGCCACCACCAGAGCAATCAACTTCACCTGAGCCAACAAAAAGGCATGCTCGAGGCAATTCCTTACAACCAGATGCCCATGTGCACCCGAACCTACCGGAGTATGATGAATTGGCAGCCCGAATTGCAGCTCTTAGAGGAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

356

Amino Acids

40.2

Weight (kDa)

6.58

Isoelectric Point (pI)

72.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 20 - 97 1.9e-22 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 713
Acc36I ACCTGC 2 cut(s) 713, 894
AccB1I GGYRCC 1 cut(s) 583
AccB7I CCANNNNNTGG 1 cut(s) 725
AciI CCGC 1 cut(s) 209
AclWI GGATC 2 cut(s) 552, 740
AcsI RAATTY 1 cut(s) 215
AcuI CTGAAG 1 cut(s) 90
AfaI GTAC 2 cut(s) 252, 392
AfiI CCNNNNNNNGG 4 cut(s) 238, 359, 722, 725
AgsI TTSAA 3 cut(s) 14, 220, 540
AjnI CCWGG 1 cut(s) 662
AluBI AGCT 4 cut(s) 86, 319, 459, 1055
AluI AGCT 4 cut(s) 86, 319, 459, 1055
Alw21I GWGCWC 1 cut(s) 1011
Alw26I GTCTC 2 cut(s) 369, 441
Alw44I GTGCAC 1 cut(s) 1007
AlwI GGATC 2 cut(s) 552, 740
Ama87I CYCGRG 1 cut(s) 975
AoxI GGCC 1 cut(s) 228
ApaLI GTGCAC 1 cut(s) 1007
ApeKI GCWGC 7 cut(s) 107, 110, 349, 900, 917, 1040, 1052
ApoI RAATTY 1 cut(s) 215
AseI ATTAAT 1 cut(s) 257
AspS9I GGNCC 1 cut(s) 229
AsuHPI GGTGA 5 cut(s) 452, 682, 740, 801, 942
AvaI CYCGRG 1 cut(s) 975
BaeGI GKGCMC 2 cut(s) 588, 1011
BanI GGYRCC 1 cut(s) 583
Bbv12I GWGCWC 1 cut(s) 1011
BbvI GCAGC 7 cut(s) 94, 122, 361, 912, 929, 1052, 1064
BccI CCATC 2 cut(s) 437, 770
BciT130I CCWGG 1 cut(s) 664
BcoDI GTCTC 2 cut(s) 369, 441
BfmI CTRYAG 1 cut(s) 108
BfuAI ACCTGC 2 cut(s) 713, 894
BisI GCNGC 7 cut(s) 108, 111, 350, 901, 918, 1041, 1053
BlsI GCNGC 7 cut(s) 109, 112, 351, 902, 919, 1042, 1054
Bme1390I CCNGG 1 cut(s) 664
BmeT110I CYCGRG 1 cut(s) 975
BmgT120I GGNCC 1 cut(s) 229
BmiI GGNNCC 3 cut(s) 231, 585, 660
BmrFI CCNGG 1 cut(s) 664
BmsI GCATC 5 cut(s) 101, 488, 777, 929, 988
BplI GAGNNNNNCTC 2 cut(s) 281, 313
Bpu10I CCTNAGC 1 cut(s) 954
BsaAI YACGTR 1 cut(s) 357
BsaI GGTCTC 1 cut(s) 369
BsaJI CCNNGG 4 cut(s) 300, 561, 662, 749
BsaWI WCCGGW 1 cut(s) 1021
BsaXI ACNNNNNCTCC 2 cut(s) 316, 346
Bsc4I CCNNNNNNNGG 4 cut(s) 238, 359, 722, 725
Bse1I ACTGG 2 cut(s) 166, 859
BseBI CCWGG 1 cut(s) 664
BseDI CCNNGG 4 cut(s) 300, 561, 662, 749
BseGI GGATG 3 cut(s) 571, 760, 781
BseLI CCNNNNNNNGG 4 cut(s) 238, 359, 722, 725
BseMII CTCAG 3 cut(s) 160, 647, 945
BseNI ACTGG 2 cut(s) 166, 859
BseSI GKGCMC 2 cut(s) 588, 1011
BseXI GCAGC 7 cut(s) 94, 122, 361, 912, 929, 1052, 1064
BsgI GTGCAG 1 cut(s) 591
BshFI GGCC 1 cut(s) 230
BshNI GGYRCC 1 cut(s) 583
BsiHKAI GWGCWC 1 cut(s) 1011
BsiHKCI CYCGRG 1 cut(s) 975
BsiSI CCGG 1 cut(s) 1022
BslFI GGGAC 2 cut(s) 308, 499
BslI CCNNNNNNNGG 4 cut(s) 238, 359, 722, 725
BsmAI GTCTC 2 cut(s) 369, 441
BsmFI GGGAC 2 cut(s) 308, 499
BsmI GAATGC 1 cut(s) 80
BsnI GGCC 1 cut(s) 230
Bso31I GGTCTC 1 cut(s) 369
BsoBI CYCGRG 1 cut(s) 975
Bsp1286I GDGCHC 2 cut(s) 588, 1011
Bsp1407I TGTACA 1 cut(s) 390
Bsp143I GATC 3 cut(s) 395, 557, 745
BspACI CCGC 1 cut(s) 209
BspANI GGCC 1 cut(s) 230
BspCNI CTCAG 3 cut(s) 159, 646, 946
BspLI GGNNCC 3 cut(s) 231, 585, 660
BspMAI CTGCAG 1 cut(s) 112
BspMI ACCTGC 2 cut(s) 713, 894
BspPI GGATC 2 cut(s) 552, 740
BspQI GCTCTTC 1 cut(s) 423
BspT107I GGYRCC 1 cut(s) 583
BspTNI GGTCTC 1 cut(s) 369
BsrGI TGTACA 1 cut(s) 390
BsrI ACTGG 2 cut(s) 166, 859
BssECI CCNNGG 4 cut(s) 300, 561, 662, 749
BssMI GATC 3 cut(s) 395, 557, 745
BssT1I CCWWGG 1 cut(s) 300
Bst2UI CCWGG 1 cut(s) 664
Bst4CI ACNGT 2 cut(s) 797, 807
Bst6I CTCTTC 4 cut(s) 419, 423, 595, 756
BstAUI TGTACA 1 cut(s) 390
BstBAI YACGTR 1 cut(s) 357
BstC8I GCNNGC 2 cut(s) 823, 972
BstDEI CTNAG 4 cut(s) 146, 633, 954, 1058
BstF5I GGATG 3 cut(s) 571, 760, 781
BstKTI GATC 3 cut(s) 398, 560, 748
BstMAI GTCTC 2 cut(s) 369, 441
BstMBI GATC 3 cut(s) 395, 557, 745
BstMWI GCNNNNNNNGC 3 cut(s) 136, 787, 1049
BstNI CCWGG 1 cut(s) 664
BstNSI RCATGY 1 cut(s) 974
BstSCI CCNGG 1 cut(s) 662
BstSFI CTRYAG 1 cut(s) 108
BstSLI GKGCMC 2 cut(s) 588, 1011
BstSNI TACGTA 1 cut(s) 357
BstV1I GCAGC 7 cut(s) 94, 122, 361, 912, 929, 1052, 1064
BstX2I RGATCY 1 cut(s) 557
BstXI CCANNNNNNTGG 1 cut(s) 307
BstYI RGATCY 1 cut(s) 557
BsuRI GGCC 1 cut(s) 230
BtsCI GGATG 3 cut(s) 571, 760, 781
BtsI GCAGTG 1 cut(s) 603
BtsIMutI CAGTG 1 cut(s) 603
BveI ACCTGC 2 cut(s) 713, 894
Cac8I GCNNGC 2 cut(s) 823, 972
Cfr13I GGNCC 1 cut(s) 229
CseI GACGC 1 cut(s) 827
Csp6I GTAC 2 cut(s) 251, 391
CviAII CATG 7 cut(s) 263, 518, 680, 737, 971, 1004, 1068
CviQI GTAC 2 cut(s) 251, 391
DdeI CTNAG 4 cut(s) 146, 633, 954, 1058
DpnI GATC 3 cut(s) 397, 559, 747
DpnII GATC 3 cut(s) 395, 557, 745
Eam1104I CTCTTC 4 cut(s) 419, 423, 595, 756
EarI CTCTTC 4 cut(s) 419, 423, 595, 756
Eco105I TACGTA 1 cut(s) 357
Eco130I CCWWGG 1 cut(s) 300
Eco31I GGTCTC 1 cut(s) 369
Eco57I CTGAAG 1 cut(s) 90
Eco88I CYCGRG 1 cut(s) 975
EcoO109I RGGNCCY 1 cut(s) 229
EcoRII CCWGG 1 cut(s) 662
EcoT14I CCWWGG 1 cut(s) 300
EcoT22I ATGCAT 2 cut(s) 264, 792
ErhI CCWWGG 1 cut(s) 300
FaeI CATG 7 cut(s) 266, 521, 683, 740, 974, 1007, 1071
FaqI GGGAC 2 cut(s) 308, 499
FatI CATG 7 cut(s) 262, 517, 679, 736, 970, 1003, 1067
Fnu4HI GCNGC 7 cut(s) 108, 111, 350, 901, 918, 1041, 1053
FokI GGATG 3 cut(s) 578, 767, 788
Fsp4HI GCNGC 7 cut(s) 108, 111, 350, 901, 918, 1041, 1053
GluI GCNGC 7 cut(s) 108, 111, 350, 901, 918, 1041, 1053
HaeIII GGCC 1 cut(s) 230
HapII CCGG 1 cut(s) 1022
HgaI GACGC 1 cut(s) 827
Hin1II CATG 7 cut(s) 266, 521, 683, 740, 974, 1007, 1071
HinfI GANTC 3 cut(s) 176, 296, 881
HpaII CCGG 1 cut(s) 1022
HphI GGTGA 5 cut(s) 452, 682, 740, 801, 942
Hpy166II GTNNAC 3 cut(s) 324, 603, 1009
Hpy188I TCNGA 8 cut(s) 124, 149, 423, 562, 636, 750, 865, 910
Hpy188III TCNNGA 2 cut(s) 68, 308
Hpy8I GTNNAC 3 cut(s) 324, 603, 1009
HpyAV CCTTC 5 cut(s) 241, 400, 661, 684, 826
HpyCH4III ACNGT 2 cut(s) 797, 807
HpyCH4IV ACGT 1 cut(s) 356
HpyF10VI GCNNNNNNNGC 3 cut(s) 136, 787, 1049
HpyF3I CTNAG 4 cut(s) 146, 633, 954, 1058
HpySE526I ACGT 1 cut(s) 356
Hsp92II CATG 7 cut(s) 266, 521, 683, 740, 974, 1007, 1071
Kzo9I GATC 3 cut(s) 395, 557, 745
LguI GCTCTTC 1 cut(s) 423
LmnI GCTCC 1 cut(s) 456
Lsp1109I GCAGC 7 cut(s) 94, 122, 361, 912, 929, 1052, 1064
LweI GCATC 5 cut(s) 101, 488, 777, 929, 988
MaeII ACGT 1 cut(s) 356
MaeIII GTNAC 4 cut(s) 140, 463, 728, 807
MalI GATC 3 cut(s) 397, 559, 747
MboI GATC 3 cut(s) 395, 557, 745
MboII GAAGA 7 cut(s) 83, 410, 415, 436, 582, 770, 773
MflI RGATCY 1 cut(s) 557
MhlI GDGCHC 2 cut(s) 588, 1011
MluCI AATT 5 cut(s) 215, 254, 982, 1034, 1047
MlyI GAGTC 1 cut(s) 290
MmeI TCCRAC 1 cut(s) 359
Mph1103I ATGCAT 2 cut(s) 264, 792
MseI TTAA 1 cut(s) 257
MslI CAYNNNNRTG 1 cut(s) 741
MspI CCGG 1 cut(s) 1022
MspR9I CCNGG 1 cut(s) 664
Mva1269I GAATGC 1 cut(s) 80
MvaI CCWGG 1 cut(s) 664
MwoI GCNNNNNNNGC 3 cut(s) 136, 787, 1049
NdeII GATC 3 cut(s) 395, 557, 745
NlaIII CATG 7 cut(s) 266, 521, 683, 740, 974, 1007, 1071
NlaIV GGNNCC 3 cut(s) 231, 585, 660
NmuCI GTSAC 3 cut(s) 140, 728, 807
NsiI ATGCAT 2 cut(s) 264, 792
NspI RCATGY 1 cut(s) 974
PaeI GCATGC 1 cut(s) 974
PaeR7I CTCGAG 1 cut(s) 975
PaqCI CACCTGC 1 cut(s) 713
PciSI GCTCTTC 1 cut(s) 423
PctI GAATGC 1 cut(s) 80
PfeI GAWTC 2 cut(s) 176, 881
PflMI CCANNNNNTGG 1 cut(s) 725
PkrI GCNGC 7 cut(s) 109, 112, 351, 902, 919, 1042, 1054
PleI GAGTC 1 cut(s) 290
PpsI GAGTC 1 cut(s) 290
Ppu21I YACGTR 1 cut(s) 357
PshBI ATTAAT 1 cut(s) 257
Psp6I CCWGG 1 cut(s) 662
PspGI CCWGG 1 cut(s) 662
PspN4I GGNNCC 3 cut(s) 231, 585, 660
PspPI GGNCC 1 cut(s) 229
PspXI VCTCGAGB 1 cut(s) 975
PstI CTGCAG 1 cut(s) 112
PsuI RGATCY 1 cut(s) 557
RsaI GTAC 2 cut(s) 252, 392
RsaNI GTAC 2 cut(s) 251, 391
RseI CAYNNNNRTG 1 cut(s) 741
SapI GCTCTTC 1 cut(s) 423
SaqAI TTAA 1 cut(s) 257
SatI GCNGC 7 cut(s) 108, 111, 350, 901, 918, 1041, 1053
Sau3AI GATC 3 cut(s) 395, 557, 745
Sau96I GGNCC 1 cut(s) 229
SchI GAGTC 1 cut(s) 290
ScrFI CCNGG 1 cut(s) 664
SduI GDGCHC 2 cut(s) 588, 1011
SfaNI GCATC 5 cut(s) 101, 488, 777, 929, 988
SfcI CTRYAG 1 cut(s) 108
Sfr274I CTCGAG 1 cut(s) 975
SlaI CTCGAG 1 cut(s) 975
SmiMI CAYNNNNRTG 1 cut(s) 741
SmlI CTYRAG 1 cut(s) 975
SmoI CTYRAG 1 cut(s) 975
SnaBI TACGTA 1 cut(s) 357
SphI GCATGC 1 cut(s) 974
Sse9I AATT 5 cut(s) 215, 254, 982, 1034, 1047
SsiI CCGC 1 cut(s) 209
StyD4I CCNGG 1 cut(s) 662
StyI CCWWGG 1 cut(s) 300
TaaI ACNGT 2 cut(s) 797, 807
TaiI ACGT 1 cut(s) 359
TaqI TCGA 3 cut(s) 288, 710, 976
TaqII GACCGA 1 cut(s) 540
TasI AATT 5 cut(s) 215, 254, 982, 1034, 1047
TatI WGTACW 1 cut(s) 390
TfiI GAWTC 2 cut(s) 176, 881
Tru1I TTAA 1 cut(s) 257
Tru9I TTAA 1 cut(s) 257
TscAI CASTG 1 cut(s) 610
TseFI GTSAC 3 cut(s) 140, 728, 807
TseI GCWGC 7 cut(s) 107, 110, 349, 900, 917, 1040, 1052
Tsp45I GTSAC 3 cut(s) 140, 728, 807
TspDTI ATGAA 8 cut(s) 260, 513, 582, 771, 845, 882, 894, 1047
TspGWI ACGGA 2 cut(s) 357, 469
TspRI CASTG 1 cut(s) 610
Van91I CCANNNNNTGG 1 cut(s) 725
VneI GTGCAC 1 cut(s) 1007
VspI ATTAAT 1 cut(s) 257
XapI RAATTY 1 cut(s) 215
XceI RCATGY 1 cut(s) 974
XhoI CTCGAG 1 cut(s) 975
Zsp2I ATGCAT 2 cut(s) 264, 792
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.