FvH4_6g31290

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0003526
Physical Location & Seq
Reverse (-)
24448691 .. 24452396
3706 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g31290.t2

Sequence Viewer

Length: 1347 bp
ATGTTTGGGTTCAGAAAGCACAAATTCACCACTAAATGCAAAACTGATATAAAGACCTTGAAGGTGAGGCTTGAGGCCATCAAGAAGAAAAGGAACTCTGTGCTCAAGTATTTGAAGAATGATGTTGCTGATCTTCTCAAGAATGGCCTCGACATTAATGCATATGGGAGGGCTGAGGGAGTTCTTGTTGAGCACAATATGACTACCTGTTATCAACTAACGGAGAGCTTTCTCGACTGTATCTTAAGTAATCTTTCTCAAATGCAGAATCAGAGCGAATGTCCTGAGGAGTGCAAGGAGGCTGTTTCGTCTGTAATATATGCCACAGCAAGATTTTCTGATTTGCCAGAACTGCGTGACCTCAGAAACATTTTCATTGAAAAATATGGAGATTCCATACAAGCTTTTGTAAATAAAGAGCTTGTGGACAAGTTCAAGTCAAATCCCCCTACAAAGGAGATGAAGGTACAATTAATGCATCACATAGCGAAAGAACACTCAATCGAATGGGGCTCCAAGGCTCTGGAACAAAAGCTGTTTACTCCACCTTCAACAAACAAGGTGCAGCCTCGGTATGGATCTCAATGTGATGGTGATGATGATATGTACAGATCACAGAAGAAGGATAATGCTATTCCGAAGAGAAAAAATAAGGCTGATGGAGACAGACGGAGCTATGTAACTGATGATGCAAGCAAGTATGCCTCTAATGATAAATACATGCTGCATAGCAGTAGTGATGATGAAGCAACAGATATCTCCCATGATGGACGAATGACTGCCTCAAGTTCAGTAGGAAGTAGATCCGAGGATGAAGCTGAAAATAAGGTGCCCTCTTCTTATAAATTCGCTCCGCCCCCTTACTACAAACCAAAGCCTCGAAAAAGAGAAAGTGGCTTTGAGGAACCCAGGAAGGTGAATGGTCATGTTGAAGACAATGCAAAACCAAGGTCAGTTAGGAAAACCTCGAAACCACAAGCAGGTTTTGACAATCATGGAAGTGATCCGAGGGATGAAAAAGAGAGAGTAATGGATGGGCTTTTGATGCGTTACAGTCAGAAACAGTCACCCTATGACTCAAGCAACATGAAGGGACATCTGAAACCTCGCAACAACCAATCAGATGAAGATACTAATGAATCCACAAGGCACAGAAGCAGCAGGTCTGAAAGAAATGTGCCCGCTGGCAGAGCGGCATCTCTGCCACCACCAGAGCCATCAGCTTCACCTGAACCAACAAAAAGGAGTGCTCGAGCCAATTCCTTTCAACCAGAGTCACATGTGCACCCAAGACTACCGGAGTATGATGACTTGGCAGCCCGGATTGCAGCTCTAAAAGGAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

449

Amino Acids

50.8

Weight (kDa)

9.09

Isoelectric Point (pI)

57.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 1 - 102 1.2e-29 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 843
Acc36I ACCTGC 2 cut(s) 971, 1152
AccB1I GGYRCC 1 cut(s) 829
AccBSI CCGCTC 1 cut(s) 1193
AciI CCGC 3 cut(s) 854, 1182, 1193
AclWI GGATC 3 cut(s) 586, 798, 998
AcsI RAATTY 2 cut(s) 23, 845
AfaI GTAC 2 cut(s) 468, 608
AfiI CCNNNNNNNGG 3 cut(s) 454, 575, 980
AflII CTTAAG 1 cut(s) 244
AflIII ACRYGT 1 cut(s) 1279
AgsI TTSAA 7 cut(s) 61, 115, 380, 436, 552, 932, 1268
AjnI CCWGG 1 cut(s) 908
AluBI AGCT 8 cut(s) 228, 404, 421, 535, 675, 818, 1223, 1331
AluI AGCT 8 cut(s) 228, 404, 421, 535, 675, 818, 1223, 1331
Alw21I GWGCWC 4 cut(s) 105, 195, 1252, 1287
Alw26I GTCTC 1 cut(s) 657
Alw44I GTGCAC 1 cut(s) 1283
AlwI GGATC 3 cut(s) 586, 798, 998
Ama87I CYCGRG 1 cut(s) 1251
AoxI GGCC 2 cut(s) 75, 145
ApaLI GTGCAC 1 cut(s) 1283
ApeKI GCWGC 5 cut(s) 565, 724, 1158, 1316, 1328
ApoI RAATTY 2 cut(s) 23, 845
ArsI GACNNNNNNTTYG 2 cut(s) 935, 967
AseI ATTAAT 2 cut(s) 156, 473
AsuC2I CCSGG 1 cut(s) 1321
AsuHPI GGTGA 6 cut(s) 19, 76, 605, 928, 1059, 1218
AvaI CYCGRG 1 cut(s) 1251
AxyI CCTNAGG 1 cut(s) 285
BaeGI GKGCMC 3 cut(s) 834, 1182, 1287
BanI GGYRCC 1 cut(s) 829
BanII GRGCYC 1 cut(s) 515
BbsI GAAGAC 1 cut(s) 939
Bbv12I GWGCWC 4 cut(s) 105, 195, 1252, 1287
BbvCI CCTCAGC 1 cut(s) 174
BbvI GCAGC 5 cut(s) 577, 711, 1170, 1328, 1340
BccI CCATC 6 cut(s) 86, 584, 653, 761, 1028, 1225
BcgI CGANNNNNNTGC 2 cut(s) 140, 174
BciT130I CCWGG 1 cut(s) 910
BcnI CCSGG 1 cut(s) 1321
BcoDI GTCTC 1 cut(s) 657
BfrI CTTAAG 1 cut(s) 244
BfuAI ACCTGC 2 cut(s) 971, 1152
BisI GCNGC 6 cut(s) 566, 725, 1159, 1194, 1317, 1329
BlsI GCNGC 6 cut(s) 567, 726, 1160, 1195, 1318, 1330
Bme1390I CCNGG 2 cut(s) 910, 1321
BmeT110I CYCGRG 1 cut(s) 1251
BmiI GGNNCC 3 cut(s) 514, 831, 906
BmrFI CCNGG 2 cut(s) 910, 1321
BmsI GCATC 4 cut(s) 487, 679, 1035, 1205
BpiI GAAGAC 1 cut(s) 939
Bpu10I CCTNAGC 1 cut(s) 174
BpuEI CTTGAG 5 cut(s) 89, 92, 122, 769, 1063
BpuMI CCSGG 1 cut(s) 1321
BsaJI CCNNGG 6 cut(s) 516, 569, 807, 908, 947, 1007
BsaWI WCCGGW 1 cut(s) 1297
BsaXI ACNNNNNCTCC 2 cut(s) 171, 201
Bsc4I CCNNNNNNNGG 3 cut(s) 454, 575, 980
Bse21I CCTNAGG 1 cut(s) 285
BseBI CCWGG 1 cut(s) 910
BseDI CCNNGG 6 cut(s) 516, 569, 807, 908, 947, 1007
BseGI GGATG 3 cut(s) 817, 1018, 1039
BseLI CCNNNNNNNGG 3 cut(s) 454, 575, 980
BseMII CTCAG 3 cut(s) 165, 276, 376
BseRI GAGGAG 1 cut(s) 302
BseSI GKGCMC 3 cut(s) 834, 1182, 1287
BseXI GCAGC 5 cut(s) 577, 711, 1170, 1328, 1340
BsgI GTGCAG 1 cut(s) 584
BshFI GGCC 2 cut(s) 77, 147
BshNI GGYRCC 1 cut(s) 829
BsiHKAI GWGCWC 4 cut(s) 105, 195, 1252, 1287
BsiHKCI CYCGRG 1 cut(s) 1251
BsiSI CCGG 2 cut(s) 1298, 1321
BslFI GGGAC 1 cut(s) 1107
BslI CCNNNNNNNGG 3 cut(s) 454, 575, 980
BsmAI GTCTC 1 cut(s) 657
BsmFI GGGAC 1 cut(s) 1107
BsnI GGCC 2 cut(s) 77, 147
BsoBI CYCGRG 1 cut(s) 1251
Bsp1286I GDGCHC 7 cut(s) 105, 195, 515, 834, 1182, 1252, 1287
Bsp1407I TGTACA 1 cut(s) 606
Bsp143I GATC 5 cut(s) 130, 578, 611, 803, 1003
BspACI CCGC 3 cut(s) 854, 1182, 1193
BspANI GGCC 2 cut(s) 77, 147
BspCNI CTCAG 3 cut(s) 166, 277, 375
BspLI GGNNCC 3 cut(s) 514, 831, 906
BspMI ACCTGC 2 cut(s) 971, 1152
BspPI GGATC 3 cut(s) 586, 798, 998
BspT107I GGYRCC 1 cut(s) 829
BspTI CTTAAG 1 cut(s) 244
BsrBI CCGCTC 1 cut(s) 1193
BsrGI TGTACA 1 cut(s) 606
BssECI CCNNGG 6 cut(s) 516, 569, 807, 908, 947, 1007
BssMI GATC 5 cut(s) 130, 578, 611, 803, 1003
BssT1I CCWWGG 2 cut(s) 516, 947
Bst2UI CCWGG 1 cut(s) 910
Bst4CI ACNGT 3 cut(s) 239, 1055, 1065
Bst6I CTCTTC 2 cut(s) 635, 841
BstAFI CTTAAG 1 cut(s) 244
BstAUI TGTACA 1 cut(s) 606
BstC8I GCNNGC 3 cut(s) 694, 1182, 1186
BstDEI CTNAG 3 cut(s) 174, 285, 362
BstF5I GGATG 3 cut(s) 817, 1018, 1039
BstKTI GATC 5 cut(s) 133, 581, 614, 806, 1006
BstMAI GTCTC 1 cut(s) 657
BstMBI GATC 5 cut(s) 130, 578, 611, 803, 1003
BstMWI GCNNNNNNNGC 4 cut(s) 352, 1045, 1190, 1325
BstNI CCWGG 1 cut(s) 910
BstNSI RCATGY 2 cut(s) 724, 1283
BstSCI CCNGG 2 cut(s) 908, 1319
BstSLI GKGCMC 3 cut(s) 834, 1182, 1287
BstV1I GCAGC 5 cut(s) 577, 711, 1170, 1328, 1340
BstV2I GAAGAC 1 cut(s) 939
BstX2I RGATCY 2 cut(s) 578, 803
BstXI CCANNNNNNTGG 1 cut(s) 523
BstYI RGATCY 2 cut(s) 578, 803
Bsu36I CCTNAGG 1 cut(s) 285
BsuRI GGCC 2 cut(s) 77, 147
BtsCI GGATG 3 cut(s) 817, 1018, 1039
BveI ACCTGC 2 cut(s) 971, 1152
Cac8I GCNNGC 3 cut(s) 694, 1182, 1186
Csp6I GTAC 2 cut(s) 467, 607
CviAII CATG 6 cut(s) 721, 764, 926, 995, 1087, 1280
CviQI GTAC 2 cut(s) 467, 607
DdeI CTNAG 3 cut(s) 174, 285, 362
DpnI GATC 5 cut(s) 132, 580, 613, 805, 1005
DpnII GATC 5 cut(s) 130, 578, 611, 803, 1003
Eam1104I CTCTTC 2 cut(s) 635, 841
EarI CTCTTC 2 cut(s) 635, 841
EciI GGCGGA 1 cut(s) 843
Eco130I CCWWGG 2 cut(s) 516, 947
Eco24I GRGCYC 1 cut(s) 515
Eco32I GATATC 1 cut(s) 757
Eco81I CCTNAGG 1 cut(s) 285
Eco88I CYCGRG 1 cut(s) 1251
EcoRII CCWGG 1 cut(s) 908
EcoRV GATATC 1 cut(s) 757
EcoT14I CCWWGG 2 cut(s) 516, 947
EcoT22I ATGCAT 2 cut(s) 163, 480
EcoT38I GRGCYC 1 cut(s) 515
ErhI CCWWGG 2 cut(s) 516, 947
FaeI CATG 6 cut(s) 724, 767, 929, 998, 1090, 1283
FaqI GGGAC 1 cut(s) 1107
FatI CATG 6 cut(s) 720, 763, 925, 994, 1086, 1279
FauI CCCGC 1 cut(s) 1189
FauNDI CATATG 1 cut(s) 163
Fnu4HI GCNGC 6 cut(s) 566, 725, 1159, 1194, 1317, 1329
FokI GGATG 3 cut(s) 824, 1025, 1046
FriOI GRGCYC 1 cut(s) 515
Fsp4HI GCNGC 6 cut(s) 566, 725, 1159, 1194, 1317, 1329
GluI GCNGC 6 cut(s) 566, 725, 1159, 1194, 1317, 1329
HaeIII GGCC 2 cut(s) 77, 147
HapII CCGG 2 cut(s) 1298, 1321
Hin1II CATG 6 cut(s) 724, 767, 929, 998, 1090, 1283
HindIII AAGCTT 1 cut(s) 402
HinfI GANTC 5 cut(s) 268, 392, 1076, 1139, 1274
HpaII CCGG 2 cut(s) 1298, 1321
HphI GGTGA 6 cut(s) 19, 76, 605, 928, 1059, 1218
Hpy166II GTNNAC 3 cut(s) 427, 540, 1285
Hpy188III TCNNGA 5 cut(s) 82, 139, 233, 284, 524
Hpy8I GTNNAC 3 cut(s) 427, 540, 1285
HpyAV CCTTC 6 cut(s) 55, 457, 558, 616, 907, 1084
HpyCH4III ACNGT 3 cut(s) 239, 1055, 1065
HpyF10VI GCNNNNNNNGC 4 cut(s) 352, 1045, 1190, 1325
HpyF3I CTNAG 3 cut(s) 174, 285, 362
Hsp92II CATG 6 cut(s) 724, 767, 929, 998, 1090, 1283
Kzo9I GATC 5 cut(s) 130, 578, 611, 803, 1003
LmnI GCTCC 3 cut(s) 518, 672, 856
Lsp1109I GCAGC 5 cut(s) 577, 711, 1170, 1328, 1340
LweI GCATC 4 cut(s) 487, 679, 1035, 1205
MaeIII GTNAC 5 cut(s) 356, 679, 1049, 1065, 1275
MalI GATC 5 cut(s) 132, 580, 613, 805, 1005
MbiI CCGCTC 1 cut(s) 1193
MboI GATC 5 cut(s) 130, 578, 611, 803, 1003
MboII GAAGA 8 cut(s) 97, 125, 127, 631, 652, 828, 944, 1139
MflI RGATCY 2 cut(s) 578, 803
MhlI GDGCHC 7 cut(s) 105, 195, 515, 834, 1182, 1252, 1287
MluCI AATT 4 cut(s) 23, 470, 845, 1258
MlyI GAGTC 2 cut(s) 1070, 1283
Mph1103I ATGCAT 2 cut(s) 163, 480
MseI TTAA 3 cut(s) 156, 245, 473
MslI CAYNNNNRTG 1 cut(s) 999
MspA1I CMGCKG 1 cut(s) 1184
MspCI CTTAAG 1 cut(s) 244
MspI CCGG 2 cut(s) 1298, 1321
MspR9I CCNGG 2 cut(s) 910, 1321
MvaI CCWGG 1 cut(s) 910
MwoI GCNNNNNNNGC 4 cut(s) 352, 1045, 1190, 1325
NciI CCSGG 1 cut(s) 1321
NdeI CATATG 1 cut(s) 163
NdeII GATC 5 cut(s) 130, 578, 611, 803, 1003
NlaIII CATG 6 cut(s) 724, 767, 929, 998, 1090, 1283
NlaIV GGNNCC 3 cut(s) 514, 831, 906
NmuCI GTSAC 3 cut(s) 356, 1065, 1275
NsiI ATGCAT 2 cut(s) 163, 480
NspI RCATGY 2 cut(s) 724, 1283
PaeR7I CTCGAG 1 cut(s) 1251
PciI ACATGT 1 cut(s) 1279
PfeI GAWTC 3 cut(s) 268, 392, 1139
PkrI GCNGC 6 cut(s) 567, 726, 1160, 1195, 1318, 1330
PleI GAGTC 2 cut(s) 1070, 1282
PpsI GAGTC 2 cut(s) 1070, 1282
PscI ACATGT 1 cut(s) 1279
PshBI ATTAAT 2 cut(s) 156, 473
PsiI TTATAA 1 cut(s) 843
Psp6I CCWGG 1 cut(s) 908
PspGI CCWGG 1 cut(s) 908
PspN4I GGNNCC 3 cut(s) 514, 831, 906
PspXI VCTCGAGB 1 cut(s) 1251
PsuI RGATCY 2 cut(s) 578, 803
RsaI GTAC 2 cut(s) 468, 608
RsaNI GTAC 2 cut(s) 467, 607
RseI CAYNNNNRTG 1 cut(s) 999
SaqAI TTAA 3 cut(s) 156, 245, 473
SatI GCNGC 6 cut(s) 566, 725, 1159, 1194, 1317, 1329
Sau3AI GATC 5 cut(s) 130, 578, 611, 803, 1003
SchI GAGTC 2 cut(s) 1070, 1283
ScrFI CCNGG 2 cut(s) 910, 1321
SduI GDGCHC 7 cut(s) 105, 195, 515, 834, 1182, 1252, 1287
SfaNI GCATC 4 cut(s) 487, 679, 1035, 1205
Sfr274I CTCGAG 1 cut(s) 1251
SlaI CTCGAG 1 cut(s) 1251
SmiMI CAYNNNNRTG 1 cut(s) 999
SmlI CTYRAG 7 cut(s) 71, 104, 137, 244, 784, 1078, 1251
SmoI CTYRAG 7 cut(s) 71, 104, 137, 244, 784, 1078, 1251
Sse9I AATT 4 cut(s) 23, 470, 845, 1258
SsiI CCGC 3 cut(s) 854, 1182, 1193
StyD4I CCNGG 2 cut(s) 908, 1319
StyI CCWWGG 2 cut(s) 516, 947
TaaI ACNGT 3 cut(s) 239, 1055, 1065
TaqI TCGA 6 cut(s) 150, 234, 504, 880, 968, 1252
TasI AATT 4 cut(s) 23, 470, 845, 1258
TatI WGTACW 1 cut(s) 606
TauI GCSGC 1 cut(s) 1196
TfiI GAWTC 3 cut(s) 268, 392, 1139
Tru1I TTAA 3 cut(s) 156, 245, 473
Tru9I TTAA 3 cut(s) 156, 245, 473
TseFI GTSAC 3 cut(s) 356, 1065, 1275
TseI GCWGC 5 cut(s) 565, 724, 1158, 1316, 1328
Tsp45I GTSAC 3 cut(s) 356, 1065, 1275
TspDTI ATGAA 8 cut(s) 364, 476, 759, 828, 1029, 1103, 1140, 1152
TspGWI ACGGA 2 cut(s) 236, 685
Vha464I CTTAAG 1 cut(s) 244
VneI GTGCAC 1 cut(s) 1283
VspI ATTAAT 2 cut(s) 156, 473
XapI RAATTY 2 cut(s) 23, 845
XceI RCATGY 2 cut(s) 724, 1283
XhoI CTCGAG 1 cut(s) 1251
Zsp2I ATGCAT 2 cut(s) 163, 480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.