Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0002025

Physical Location & Seq

Genomic Coordinates Forward (+)

23527570 .. 23531226

Gene Structure 3657 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G63440.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2988 bp

ATGGCGACGAAGCTTTTGTCGCTTACATGCATAAGGAAAGAGAGATTCAGCGAGCGTTACCCTCTGGTGCGGAAGCACCTTACCAGGTCTCGCGACGGCGGCGGAGGATCATCGTCGGAGACGGCGGCTTTTGAGATCGATGATCCGATTTCCAGGGCGGTTTTTCAAGTGTTAGGAATGACTTGCTCTGCTTGCGCTGGATCTGTTGAAAAGGCTATCAAGCGTCTCCCTGGAATTCACGATGCTGTCATCGATGCTCTGAATAATCGTGCTCAGATTCTGTTTTACCCTAACTCTGTCGATGTGGAGACAATTCGTGAGACTATTGAGGATGCTGGATTTGAAGCATCGTTGATCGAAAATGAAGCGAATGAGAGGTCGAGACAAGTTTGTAGAATAAGAATTAATGGTATGACTTGTACTTCGTGTTCTTCAACAATCGAAAGAGTTTTGCAATCGGTTAATGGTGTACAAAGAGCACATGTGGCTTTAGCAATTGAAGAAGCTGAGATTCATTATGATCCTAGACTCTCGAGCTATGATAGACTATTGGAGGAGATAGAAAATGCTGGTTTTGAAGCTGTGCTTATAAGTACAGGCGAGGATGTGAGCAAGATTGATTTGAAGATTGATGGTGAGCTTACTGATGAATCCATGAAGGTTATTGAAAGGTCGCTTGAAGCACTTCCTGGTGTTCAAAGTGTTGAGATCAGCCATGGAACTGATAAGATATCTGTATTGTACAAACCTGATGTGACGGGGCCGAGGAATTTCATTCAGGTGATTGAGTCTACTGTCTTTGGTCATAGTGGTCACATCAAGGCAACAATATTCTCAGAGGGAGGGGTAGGCAGAGAATCTCAAAAGCAAGGGGAGATTAAGCAGTATTATAAGTCGTTTCTCTGGAGTTTGGTTTTTACAGTACCGGTGTTTTTGACAGCCATGGTCTTTATGTATATCCCTGGAATTAAAGATTTGCTCATGTTTAAGGTCATCAATATGCTCACCGTTGGAGAAATCATAAGGTGTGTTTTGGCTACTCCTGTCCAGTTTGTCATTGGTTGGAGATTTTATACTGGCTCTTACAAGGCTTTACGCCGAGGATCGGCTAATATGGATGTTCTGATTGCTCTGGGGACAAATGCAGCTTATTTCTATTCATTATACACAGTGTTGAGAGCTGCAACATCTCCTGATTTCAAGGGAGTAGATTTCTTTGAGACTAGTGCCATGCTCATTTCCTTTATCATACTAGGAAAATATTTGGAGGTTATGGCTAAAGGAAAAACATCTCAAGCGATCGCAAAGCTTATGAACTTGGCACCCGACACTGCGATATTGTTGAGTTTGGACAAGGAAGGGAATGTGACTGGTGAAGAGGAGATTGATGGTCGATTGATACAGAAGAATGACGTGATCAAAATTGTTCCTGGTGCTAAAGTAGCTTCAGATGGTTATGTCATATGGGGACAAAGTCATGTGAATGAAAGTATGATCACTGGAGAGGCTAGGCCAGTGGCAAAGAGAAAGGGTGATACAGTTATAGGAGGCACTTTGAACGAGAATGGTGTTCTGCATGTTAAGGTTACAAGGGTTGGTTCAGAGAGTGCTCTTGCTCAGATTGTTCGACTTGTTGAGTCCGCACAACTGGCCAAAGCTCCAGTACAGAAGTTGGCTGATCGGATTTCCAAGTTCTTTGTTCCTCTTGTAATTTTCCTCTCGTTCTCAACTTGGCTTGCCTGGTTCTTAGCTGGGAAACTGCATTGGTACCCTGAATCCTGGATACCTTCTTCGATGGATAGCTTTGAGCTAGCTCTTCAGTTCGGGATCTCTGTCATGGTCATAGCTTGTCCATGTGCTCTTGGGCTGGCTACTCCAACTGCTGTTATGGTTGGTACTGGGGTTGGTGCATCCCAAGGTGTGCTGATAAAGGGTGGCCAAGCCCTAGAAAGAGCACACAAGGTAAATTGCATTGTCTTTGACAAGACAGGAACTCTCACGATGGGGAAGCCCGTTGTTGTGAAAACAAAGCTCCTGAAAAACATGGTACTTCGAGAGTTCTATGAACTTGTTGCTGCAACTGAGGTAAACAGTGAGCATCCGTTAGCAAAGGCCATTGTCGAGTACGCGAAGAAATTCAGAGACGACGAAGAGAACCCTGCCTGGCCAGAAGCCTGTGATTTTGTGTCCATCACTGGAAAGGGAGTGAAAGCCACCGTTAAAGGTAGAGAGATTATGGTGGGGAACAAGAATCTGATGAATGATCATAAAGTTATTATTCCAGATGATGCTGAAGAGTTGCTAGCTGACTCTGAAGATATGGCCCAGACCGGAATTCTTGTCTCCATAAACAGTGAACTGATTGGAGTTTTGTCTGTTTCGGATCCTCTAAAACCGAGTGCTCGAGAAGCCATCTCCATTCTAAAATCCATGAATATCAAAAGCATCATGGTAACTGGTGACAACTGGGGAACAGCAAACTCAATTGCTAGAGAAGTCGGTATCGACTCTGTTATCGCAGAAGCTAAACCTGAGCAAAAAGCAGAGAAAGTCAAGGAATTACAGGCTGCGGGACATGTTGTGGCAATGGTAGGTGACGGAATCAATGACTCACCGGCTCTCGTGGCAGCGGATGTAGGTATGGCGATAGGTGCAGGAACAGACATTGCTATAGAAGCAGCGGATATAGTTCTGATGAAAAGCAACTTAGAAGATGTGATCACAGCCATTGATCTTTCAAGGAAAACGTTCTCAAGAATCCGTCTCAACTACGTATGGGCTCTCGGGTATAACCTCATGGGGATACCGATCGCTGCGGGAGTGCTTTTCCCAGGGACACGTTTCAGGTTGCCTCCATGGATTGCAGGTGCTGCAATGGCTGCTTCTTCTGTTAGTGTTGTGTGTTGCTCTCTCTTGCTTAAGAACTACAAGCGACCTAAGAAGCTTGATCATCTGGAGATTCGGGAGATTCAGGTGGAGCGAGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0008150 GO:0008324 GO:0009636 GO:0010035 GO:0010038 GO:0010273 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042221 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046688 GO:0046873 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0061687 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0097501 GO:0098655 GO:0098660 GO:0098662 GO:0098754 GO:0099131 GO:0099132 GO:1990169

KEGG Pathways

Metabolic & Signaling

ko01524 ko04016 map01524 map04016

Pfam Domains

Protein Families

E1-E2_ATPase HMA Hydrolase

Protein Analysis

995

Amino Acids

108.34

Weight (kDa)

6.05

Isoelectric Point (pI)

33.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMA	PF00403	54 - 115	3.6e-16	Heavy-metal-associated domain
HMA	PF00403	133 - 193	1.4e-12	Heavy-metal-associated domain
E1-E2_ATPase	PF00122	458 - 546	2.2e-23	P-type ATPase actuator domain
Hydrolase	PF00702	656 - 878	1e-35	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0002025)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G63440
fragaria_vesca	FvH4_5g02090 FvH4_5g02090 FvH4_5g02091 FvH4_5g02091 FvH4_5g02091 FvH4_5g02091
malus_domestica	MD06G1080800.v1.1 MD06G1080900.v1.1 MD14G1102600.v1.1
prunus_persica	Prupe.5G097900_v2.0.a1 Prupe.5G097900_v2.0.a1 Prupe.5G098000_v2.0.a1
pyrus_communis	pycom06g07780 pycom06g07790 pycom14g09890
rosa_chinensis	RchiOBHm_Chr7g0197781 RchiOBHm_Chr7g0198111 RchiOBHm_Chr7g0198141
rosa_laevigata	RLG00000003885 RLG00000003888 RLG00000019170
rosa_multiflora	Rmu_sc0000221.1_g000018 Rmu_sc0000221.1_g000030 Rmu_sc0006732.1_g000016
rosa_roxburghii	Rroxscaffold_3G00257940 Rroxscaffold_3G00257970 Rroxscaffold_3G00257990
rosa_rugosa	Rorug07G0045700 Rorug07G0045700 Rorug07G0045800 Rorug07G0045800 Rorug07G0045900
rosa_samantha	Rh7AG169100 Rh7AG169200 Rh7BG169900 Rh7BG172300 Rh7CG178600 Rh7CG178700 Rh7DG169200 Rh7DG171400 Rh7DG171800
rosa_wichuraiana	Rw7G014570 Rw7G014780 Rw7G014790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	590, 891
AarI	CACCTGC	1 cut(s)	2855
Acc36I	ACCTGC	1 cut(s)	2855
Acc65I	GGTACC	1 cut(s)	1767
AccB1I	GGYRCC	2 cut(s)	1321, 1767
AccI	GTMKAC	1 cut(s)	791
AccII	CGCG	2 cut(s)	93, 2129
AclI	AACGTT	1 cut(s)	2747
AclWI	GGATC	8 cut(s)	115, 137, 208, 515, 1111, 1835, 2378, 2391
AcoI	YGGCCR	3 cut(s)	1650, 1936, 2165
AcsI	RAATTY	4 cut(s)	234, 769, 2135, 2334
AcuI	CTGAAG	4 cut(s)	1431, 1802, 2313, 2334
AfiI	CCNNNNNNNGG	1 cut(s)	1105
AflII	CTTAAG	1 cut(s)	2918
AflIII	ACRYGT	3 cut(s)	481, 2575, 2837
AgeI	ACCGGT	1 cut(s)	925
AhlI	ACTAGT	1 cut(s)	1223
AjiI	CACGTC	1 cut(s)	1414
Alw21I	GWGCWC	6 cut(s)	274, 481, 1612, 1861, 1957, 2404
AlwI	GGATC	8 cut(s)	115, 137, 208, 515, 1111, 1835, 2378, 2391
AlwNI	CAGNNNCTG	2 cut(s)	280, 2870
Ama87I	CYCGRG	3 cut(s)	532, 2403, 2783
AoxI	GGCC	7 cut(s)	761, 1511, 1650, 1936, 2112, 2165, 2322
ApeKI	GCWGC	9 cut(s)	1145, 1181, 2075, 2567, 2627, 2678, 2813, 2870, 2879
ApoI	RAATTY	4 cut(s)	234, 769, 2135, 2334
ArsI	GACNNNNNNTTYG	6 cut(s)	30, 34, 362, 394, 406, 438
AseI	ATTAAT	1 cut(s)	405
AsiGI	ACCGGT	1 cut(s)	925
AsiSI	GCGATCGC	1 cut(s)	1302
Asp700I	GAANNNNTTC	3 cut(s)	684, 2135, 2747
Asp718I	GGTACC	1 cut(s)	1767
AspLEI	GCGC	1 cut(s)	197
AspS9I	GGNCC	2 cut(s)	761, 2323
AsuHPI	GGTGA	8 cut(s)	647, 793, 997, 1385, 1544, 2471, 2604, 2606
AsuNHI	GCTAGC	2 cut(s)	1810, 2302
AvaI	CYCGRG	3 cut(s)	532, 2403, 2783
BaeI	ACNNNNGTAYC	2 cut(s)	1887, 1920
BalI	TGGCCA	3 cut(s)	1652, 1938, 2167
BamHI	GGATCC	1 cut(s)	2383
BanI	GGYRCC	2 cut(s)	1321, 1767
BanII	GRGCYC	1 cut(s)	2782
BauI	CACGAG	1 cut(s)	2621
Bbv12I	GWGCWC	6 cut(s)	274, 481, 1612, 1861, 1957, 2404
BbvI	GCAGC	9 cut(s)	1157, 1168, 2062, 2554, 2639, 2690, 2800, 2857, 2866
BccI	CCATC	7 cut(s)	626, 1382, 1445, 1789, 1996, 2198, 2420
BceAI	ACGGC	2 cut(s)	112, 138
BciVI	GTATCC	2 cut(s)	1776, 2796
BclI	TGATCA	5 cut(s)	1416, 1494, 2263, 2718, 2947
BcuI	ACTAGT	1 cut(s)	1223
BfaI	CTAG	8 cut(s)	525, 1224, 1253, 1509, 1811, 1946, 2303, 2490
BfmI	CTRYAG	1 cut(s)	2670
BfrI	CTTAAG	1 cut(s)	2918
BfuAI	ACCTGC	1 cut(s)	2855
BfuI	GTATCC	2 cut(s)	1776, 2796
BmeT110I	CYCGRG	3 cut(s)	532, 2403, 2783
BmgBI	CACGTC	1 cut(s)	1414
BmgT120I	GGNCC	2 cut(s)	761, 2323
BmiI	GGNNCC	4 cut(s)	762, 1323, 1769, 2385
BmrI	ACTGGG	2 cut(s)	1908, 2476
BmsI	GCATC	8 cut(s)	232, 244, 322, 356, 1919, 2107, 2278, 2454
BmtI	GCTAGC	2 cut(s)	1814, 2306
BmuI	ACTGGG	2 cut(s)	1908, 2476
BpmI	CTGGAG	4 cut(s)	925, 1521, 1644, 2975
Bpu10I	CCTNAGC	1 cut(s)	2532
BpuEI	CTTGAG	2 cut(s)	1278, 2737
Bsa29I	ATCGAT	2 cut(s)	138, 252
BsaAI	YACGTR	1 cut(s)	2773
BsaBI	GATNNNNATC	1 cut(s)	2807
BsaI	GGTCTC	1 cut(s)	93
BsaWI	WCCGGW	2 cut(s)	925, 2330
Bsc4I	CCNNNNNNNGG	1 cut(s)	1105
Bse118I	RCCGGY	2 cut(s)	925, 2614
Bse3DI	GCAATG	3 cut(s)	2592, 2664, 2880
Bse8I	GATNNNNATC	1 cut(s)	2807
BseCI	ATCGAT	2 cut(s)	138, 252
BseGI	GGATG	6 cut(s)	337, 610, 1123, 1910, 2098, 2638
BseJI	GATNNNNATC	1 cut(s)	2807
BseLI	CCNNNNNNNGG	1 cut(s)	1105
BseMI	GCAATG	3 cut(s)	2592, 2664, 2880
BseMII	CTCAG	6 cut(s)	287, 498, 849, 1631, 2073, 2523
BseRI	GAGGAG	2 cut(s)	569, 1394
BseXI	GCAGC	9 cut(s)	1157, 1168, 2062, 2554, 2639, 2690, 2800, 2857, 2866
BseYI	CCCAGC	1 cut(s)	1751
BsgI	GTGCAG	1 cut(s)	2673
Bsh1236I	CGCG	2 cut(s)	93, 2129
Bsh1285I	CGRYCG	2 cut(s)	1302, 2811
BshFI	GGCC	7 cut(s)	763, 1513, 1652, 1938, 2114, 2167, 2324
BshNI	GGYRCC	2 cut(s)	1321, 1767
BshTI	ACCGGT	1 cut(s)	925
BshVI	ATCGAT	2 cut(s)	138, 252
BsiEI	CGRYCG	2 cut(s)	1302, 2811
BsiHKAI	GWGCWC	6 cut(s)	274, 481, 1612, 1861, 1957, 2404
BsiHKCI	CYCGRG	3 cut(s)	532, 2403, 2783
BsiSI	CCGG	3 cut(s)	926, 2331, 2615
BslFI	GGGAC	4 cut(s)	1150, 1482, 2586, 2848
BslI	CCNNNNNNNGG	1 cut(s)	1105
BsmBI	CGTCTC	4 cut(s)	113, 230, 2136, 2768
BsmFI	GGGAC	4 cut(s)	1150, 1482, 2586, 2848
BsnI	GGCC	7 cut(s)	763, 1513, 1652, 1938, 2114, 2167, 2324
Bso31I	GGTCTC	1 cut(s)	93
BsoBI	CYCGRG	3 cut(s)	532, 2403, 2783
Bsp1286I	GDGCHC	7 cut(s)	274, 481, 1612, 1861, 1957, 2404, 2782
Bsp1407I	TGTACA	2 cut(s)	469, 741
Bsp19I	CCATGG	3 cut(s)	715, 942, 2855
Bsp68I	TCGCGA	1 cut(s)	93
BspANI	GGCC	7 cut(s)	763, 1513, 1652, 1938, 2114, 2167, 2324
BspCNI	CTCAG	6 cut(s)	286, 499, 848, 1630, 2074, 2524
BspDI	ATCGAT	2 cut(s)	138, 252
BspFNI	CGCG	2 cut(s)	93, 2129
BspLI	GGNNCC	4 cut(s)	762, 1323, 1769, 2385
BspMI	ACCTGC	1 cut(s)	2855
BspOI	GCTAGC	2 cut(s)	1814, 2306
BspPI	GGATC	8 cut(s)	115, 137, 208, 515, 1111, 1835, 2378, 2391
BspQI	GCTCTTC	1 cut(s)	1821
BspT107I	GGYRCC	2 cut(s)	1321, 1767
BspTI	CTTAAG	1 cut(s)	2918
BspTNI	GGTCTC	1 cut(s)	93
BsrDI	GCAATG	3 cut(s)	2592, 2664, 2880
BsrFI	RCCGGY	2 cut(s)	925, 2614
BsrGI	TGTACA	2 cut(s)	469, 741
BssAI	RCCGGY	2 cut(s)	925, 2614
BssSI	CACGAG	1 cut(s)	2621
BssT1I	CCWWGG	4 cut(s)	715, 942, 1915, 2855
Bst2BI	CACGAG	1 cut(s)	2621
Bst4CI	ACNGT	8 cut(s)	796, 922, 1009, 1171, 1540, 2093, 2218, 2354
Bst6I	CTCTTC	4 cut(s)	1371, 1821, 2145, 2289
BstAFI	CTTAAG	1 cut(s)	2918
BstAPI	GCANNNNNTGC	2 cut(s)	2870, 2879
BstAUI	TGTACA	2 cut(s)	469, 741
BstBAI	YACGTR	1 cut(s)	2773
BstC8I	GCNNGC	6 cut(s)	53, 193, 1737, 1812, 1869, 2304
BstDEI	CTNAG	9 cut(s)	273, 507, 835, 1617, 1747, 2082, 2532, 2707, 2937
BstDSI	CCRYGG	3 cut(s)	715, 942, 2855
BstF5I	GGATG	6 cut(s)	337, 610, 1123, 1910, 2098, 2638
BstFNI	CGCG	2 cut(s)	93, 2129
BstHHI	GCGC	1 cut(s)	197
BstMCI	CGRYCG	2 cut(s)	1302, 2811
BstNSI	RCATGY	4 cut(s)	30, 485, 1580, 2579
BstSFI	CTRYAG	1 cut(s)	2670
BstSNI	TACGTA	1 cut(s)	2773
BstUI	CGCG	2 cut(s)	93, 2129
BstV1I	GCAGC	9 cut(s)	1157, 1168, 2062, 2554, 2639, 2690, 2800, 2857, 2866
BstX2I	RGATCY	3 cut(s)	200, 1827, 2383
BstYI	RGATCY	3 cut(s)	200, 1827, 2383
Bsu15I	ATCGAT	2 cut(s)	138, 252
BsuI	GTATCC	2 cut(s)	1776, 2796
BsuRI	GGCC	7 cut(s)	763, 1513, 1652, 1938, 2114, 2167, 2324
BsuTUI	ATCGAT	2 cut(s)	138, 252
BtgI	CCRYGG	3 cut(s)	715, 942, 2855
BtrI	CACGTC	1 cut(s)	1414
BtsCI	GGATG	6 cut(s)	337, 610, 1123, 1910, 2098, 2638
BtsI	GCAGTG	1 cut(s)	1329
BtsIMutI	CAGTG	7 cut(s)	1176, 1329, 1497, 1521, 2098, 2193, 2359
BtuMI	TCGCGA	1 cut(s)	93
BveI	ACCTGC	1 cut(s)	2855
Cac8I	GCNNGC	6 cut(s)	53, 193, 1737, 1812, 1869, 2304
CaiI	CAGNNNCTG	2 cut(s)	280, 2870
CfoI	GCGC	1 cut(s)	197
Cfr10I	RCCGGY	2 cut(s)	925, 2614
Cfr13I	GGNCC	2 cut(s)	761, 2323
ClaI	ATCGAT	2 cut(s)	138, 252
CseI	GACGC	1 cut(s)	212
CsiI	ACCWGGT	1 cut(s)	83
CspAI	ACCGGT	1 cut(s)	925
DdeI	CTNAG	9 cut(s)	273, 507, 835, 1617, 1747, 2082, 2532, 2707, 2937
EaeI	YGGCCR	3 cut(s)	1650, 1936, 2165
Eam1104I	CTCTTC	4 cut(s)	1371, 1821, 2145, 2289
EarI	CTCTTC	4 cut(s)	1371, 1821, 2145, 2289
EciI	GGCGGA	1 cut(s)	117
Eco105I	TACGTA	1 cut(s)	2773
Eco130I	CCWWGG	4 cut(s)	715, 942, 1915, 2855
Eco24I	GRGCYC	1 cut(s)	2782
Eco31I	GGTCTC	1 cut(s)	93
Eco32I	GATATC	1 cut(s)	732
Eco57I	CTGAAG	4 cut(s)	1431, 1802, 2313, 2334
Eco88I	CYCGRG	3 cut(s)	532, 2403, 2783
EcoRI	GAATTC	2 cut(s)	234, 2334
EcoRV	GATATC	1 cut(s)	732
EcoT14I	CCWWGG	4 cut(s)	715, 942, 1915, 2855
EcoT22I	ATGCAT	1 cut(s)	32
EcoT38I	GRGCYC	1 cut(s)	2782
ErhI	CCWWGG	4 cut(s)	715, 942, 1915, 2855
Esp3I	CGTCTC	4 cut(s)	113, 230, 2136, 2768
FalI	AAGNNNNNCTT	2 cut(s)	570, 602
FaqI	GGGAC	4 cut(s)	1150, 1482, 2586, 2848
FauI	CCCGC	2 cut(s)	2563, 2809
FauNDI	CATATG	1 cut(s)	1463
FbaI	TGATCA	5 cut(s)	1416, 1494, 2263, 2718, 2947
FblI	GTMKAC	1 cut(s)	791
FokI	GGATG	6 cut(s)	344, 617, 1130, 1897, 2085, 2645
FriOI	GRGCYC	1 cut(s)	2782
FspBI	CTAG	8 cut(s)	525, 1224, 1253, 1509, 1811, 1946, 2303, 2490
GlaI	GCGC	1 cut(s)	196
GsaI	CCCAGC	1 cut(s)	1755
GsuI	CTGGAG	4 cut(s)	925, 1521, 1644, 2975
HaeIII	GGCC	7 cut(s)	763, 1513, 1652, 1938, 2114, 2167, 2324
HapII	CCGG	3 cut(s)	926, 2331, 2615
HgaI	GACGC	1 cut(s)	212
HhaI	GCGC	1 cut(s)	197
Hin6I	GCGC	1 cut(s)	195
HinP1I	GCGC	1 cut(s)	195
HindIII	AAGCTT	3 cut(s)	11, 1307, 2942
HpaII	CCGG	3 cut(s)	926, 2331, 2615
HphI	GGTGA	8 cut(s)	647, 793, 997, 1385, 1544, 2471, 2604, 2606
Hpy166II	GTNNAC	4 cut(s)	470, 792, 2089, 2357
Hpy8I	GTNNAC	4 cut(s)	470, 792, 2089, 2357
Hpy99I	CGWCG	4 cut(s)	10, 98, 118, 2150
HpyAV	CCTTC	3 cut(s)	652, 1352, 1797
HpyCH4III	ACNGT	8 cut(s)	796, 922, 1009, 1171, 1540, 2093, 2218, 2354
HpyCH4IV	ACGT	4 cut(s)	1413, 2747, 2772, 2839
HpyF3I	CTNAG	9 cut(s)	273, 507, 835, 1617, 1747, 2082, 2532, 2707, 2937
HpySE526I	ACGT	4 cut(s)	1413, 2747, 2772, 2839
HspAI	GCGC	1 cut(s)	195
KpnI	GGTACC	1 cut(s)	1771
Ksp22I	TGATCA	5 cut(s)	1416, 1494, 2263, 2718, 2947
LguI	GCTCTTC	1 cut(s)	1821
LmnI	GCTCC	3 cut(s)	1663, 2037, 2977
Lsp1109I	GCAGC	9 cut(s)	1157, 1168, 2062, 2554, 2639, 2690, 2800, 2857, 2866
LweI	GCATC	8 cut(s)	232, 244, 322, 356, 1919, 2107, 2278, 2454
MabI	ACCWGGT	1 cut(s)	83
MaeI	CTAG	8 cut(s)	525, 1224, 1253, 1509, 1811, 1946, 2303, 2490
MaeII	ACGT	4 cut(s)	1413, 2747, 2772, 2839
MaeIII	GTNAC	8 cut(s)	56, 754, 812, 1366, 1585, 2452, 2459, 2594
MfeI	CAATTG	2 cut(s)	495, 2484
MflI	RGATCY	3 cut(s)	200, 1827, 2383
MhlI	GDGCHC	7 cut(s)	274, 481, 1612, 1861, 1957, 2404, 2782
MlsI	TGGCCA	3 cut(s)	1652, 1938, 2167
MluNI	TGGCCA	3 cut(s)	1652, 1938, 2167
MlyI	GAGTC	6 cut(s)	522, 797, 1646, 2303, 2501, 2603
MmeI	TCCRAC	4 cut(s)	96, 991, 1043, 1901
Mox20I	TGGCCA	3 cut(s)	1652, 1938, 2167
Mph1103I	ATGCAT	1 cut(s)	32
MroXI	GAANNNNTTC	3 cut(s)	684, 2135, 2747
MscI	TGGCCA	3 cut(s)	1652, 1938, 2167
MseI	TTAA	9 cut(s)	405, 462, 879, 969, 987, 1581, 2220, 2919, 2986
MslI	CAYNNNNRTG	3 cut(s)	779, 998, 1482
Msp20I	TGGCCA	3 cut(s)	1652, 1938, 2167
MspA1I	CMGCKG	2 cut(s)	2630, 2681
MspCI	CTTAAG	1 cut(s)	2918
MspI	CCGG	3 cut(s)	926, 2331, 2615
MunI	CAATTG	2 cut(s)	495, 2484
MvnI	CGCG	2 cut(s)	93, 2129
NcoI	CCATGG	3 cut(s)	715, 942, 2855
NdeI	CATATG	1 cut(s)	1463
NheI	GCTAGC	2 cut(s)	1810, 2302
NlaIV	GGNNCC	4 cut(s)	762, 1323, 1769, 2385
NmeAIII	GCCGAG	2 cut(s)	789, 1124
NmuCI	GTSAC	5 cut(s)	754, 812, 1366, 2459, 2594
NruI	TCGCGA	1 cut(s)	93
NsiI	ATGCAT	1 cut(s)	32
NspI	RCATGY	4 cut(s)	30, 485, 1580, 2579
PaeR7I	CTCGAG	2 cut(s)	532, 2403
PaqCI	CACCTGC	1 cut(s)	2855
PasI	CCCWGGG	1 cut(s)	2831
PciI	ACATGT	2 cut(s)	481, 2575
PciSI	GCTCTTC	1 cut(s)	1821
PdmI	GAANNNNTTC	3 cut(s)	684, 2135, 2747
PflFI	GACNNNGTC	1 cut(s)	1473
PfoI	TCCNGGA	1 cut(s)	1778
PinAI	ACCGGT	1 cut(s)	925
Ple19I	CGATCG	2 cut(s)	1302, 2811
PleI	GAGTC	6 cut(s)	522, 796, 1645, 2303, 2501, 2603
PpsI	GAGTC	6 cut(s)	522, 796, 1645, 2303, 2501, 2603
Ppu21I	YACGTR	1 cut(s)	2773
PscI	ACATGT	2 cut(s)	481, 2575
PshBI	ATTAAT	1 cut(s)	405
PsiI	TTATAA	2 cut(s)	590, 891
Psp1406I	AACGTT	1 cut(s)	2747
PspFI	CCCAGC	1 cut(s)	1751
PspN4I	GGNNCC	4 cut(s)	762, 1323, 1769, 2385
PspPI	GGNCC	2 cut(s)	761, 2323
PsrI	GAACNNNNNNTAC	2 cut(s)	412, 444
PstNI	CAGNNNCTG	2 cut(s)	280, 2870
PsuI	RGATCY	3 cut(s)	200, 1827, 2383
PsyI	GACNNNGTC	1 cut(s)	1473
PvuI	CGATCG	2 cut(s)	1302, 2811
RgaI	GCGATCGC	1 cut(s)	1302
RruI	TCGCGA	1 cut(s)	93
RseI	CAYNNNNRTG	3 cut(s)	779, 998, 1482
SapI	GCTCTTC	1 cut(s)	1821
SaqAI	TTAA	9 cut(s)	405, 462, 879, 969, 987, 1581, 2220, 2919, 2986
Sau96I	GGNCC	2 cut(s)	761, 2323
SchI	GAGTC	6 cut(s)	522, 797, 1646, 2303, 2501, 2603
SduI	GDGCHC	7 cut(s)	274, 481, 1612, 1861, 1957, 2404, 2782
SexAI	ACCWGGT	1 cut(s)	83
SfaAI	GCGATCGC	1 cut(s)	1302
SfaNI	GCATC	8 cut(s)	232, 244, 322, 356, 1919, 2107, 2278, 2454
SfcI	CTRYAG	1 cut(s)	2670
Sfr274I	CTCGAG	2 cut(s)	532, 2403
SgfI	GCGATCGC	1 cut(s)	1302
SlaI	CTCGAG	2 cut(s)	532, 2403
SmiMI	CAYNNNNRTG	3 cut(s)	779, 998, 1482
SmlI	CTYRAG	5 cut(s)	532, 1293, 2403, 2752, 2918
SmoI	CTYRAG	5 cut(s)	532, 1293, 2403, 2752, 2918
SnaBI	TACGTA	1 cut(s)	2773
SpeI	ACTAGT	1 cut(s)	1223
SspI	AATATT	2 cut(s)	831, 1262
SspMI	CTAG	8 cut(s)	525, 1224, 1253, 1509, 1811, 1946, 2303, 2490
StyI	CCWWGG	4 cut(s)	715, 942, 1915, 2855
TaaI	ACNGT	8 cut(s)	796, 922, 1009, 1171, 1540, 2093, 2218, 2354
TaiI	ACGT	4 cut(s)	1416, 2750, 2775, 2842
TatI	WGTACW	5 cut(s)	419, 469, 593, 741, 1663
TauI	GCSGC	2 cut(s)	102, 128
Tru1I	TTAA	9 cut(s)	405, 462, 879, 969, 987, 1581, 2220, 2919, 2986
Tru9I	TTAA	9 cut(s)	405, 462, 879, 969, 987, 1581, 2220, 2919, 2986
TscAI	CASTG	7 cut(s)	1176, 1336, 1504, 1521, 2098, 2200, 2359
TseFI	GTSAC	5 cut(s)	754, 812, 1366, 2459, 2594
TseI	GCWGC	9 cut(s)	1145, 1181, 2075, 2567, 2627, 2678, 2813, 2870, 2879
Tsp45I	GTSAC	5 cut(s)	754, 812, 1366, 2459, 2594
TspGWI	ACGGA	3 cut(s)	2091, 2613, 2750
TspRI	CASTG	7 cut(s)	1176, 1336, 1504, 1521, 2098, 2200, 2359
Tth111I	GACNNNGTC	1 cut(s)	1473
Vha464I	CTTAAG	1 cut(s)	2918
VspI	ATTAAT	1 cut(s)	405
XapI	RAATTY	4 cut(s)	234, 769, 2135, 2334
XceI	RCATGY	4 cut(s)	30, 485, 1580, 2579
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1055
XhoI	CTCGAG	2 cut(s)	532, 2403
XmiI	GTMKAC	1 cut(s)	791
XmnI	GAANNNNTTC	3 cut(s)	684, 2135, 2747
XspI	CTAG	8 cut(s)	525, 1224, 1253, 1509, 1811, 1946, 2303, 2490
Zsp2I	ATGCAT	1 cut(s)	32

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.