Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0002025

Physical Location & Seq

Genomic Coordinates Reverse (-)

47716857 .. 47723792

Gene Structure 6936 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000019170

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3024 bp

ATGGCGACCAAGTTATTTGCTCTTGAGTGCATACGCAACAACAATGGTGAAGGTCGCGGCGGCAGCGATATGTCGCCGCGGCCTCACTATCCCTCCATGCCAAAGTATCCGAACGGTGTGGTGGCGGAGAAGACCACTATGGTGGTGGGCACCGATTCCAAGGCAGTGTTCTCCGTCATCGGCATGACCTGCTCCGCCTGCGTGGGACCGGTAGAGAAGGCTGTGAAGAGGCTAACCGGGATTCACGAGGCCGTGGTTGACGTGCTCAACAACCGTGCCCAAGTCATGTTCTTCCCCAACTTCGTTAACGCAGAGACTATCCGTGAGACTATTGAAGATGCTGGATTTCAAGCAACATTGATTGCTGATGAGGGAAACGAGAAGTCCACGCTGGTGTGCAGAATAAGGATCAAAGCAATGACTTGCACTTCCACTGTTGAATCAGCTTTGCAGGCAGTTCATGGTGTACAAAAAGCCGAAGTTGCCTTAGCAACTGAAGAAGCAGATGTCCACTATGATCCAAAGATAGTGAGCTGCAATCAGCTGATGGTAGCCATTGAAGACAGTGGATTCGAAGCCATACTTATTAATTCAGGTGAAGACATGAGCAAGATAGATCTTAACGTTGATGGTTCTCTTCAAGCACTCCCTGGGGTGCAAGGTGTAGACATACATCATGATGACAGAAAAATTTCCCTGTCTTACAAGCCAGATATGACAGGGCCCCGAAATTTCATCAATGTGATTGAAACAACTGGATCCAGGTGTTTCAGGGCAAATATATATCCTGGCGGTGGAGCAGGAAGAGAAAATCATAGGAAGGAGGAAATTCAGCAGTACTACAGAGTCTTTCTCTGGAGTTTGATTTTCACCATTCCAGTATTTTTAACCTCCATGGTCTTCATGTACATCCCTGGACTTAAGCATGGCTTAGACAAGAAAGTAGTAAATATGCTCAGCATTGGGGAGCTTATAAGGTGGATTCTGTCTACTCCGGTGCAGTTCATCATAGGCTGGAGGTTCTATACTGGGGCATACAAGTCAGTGCGCCATGGTTCTGCTAATATGGATGTGTTAATTGCCTTGGGAACAAATGCAGCATATTTCTATTCAGTCTACTCCGTACTGAGAGCTGCCACCTCCCCACATTTTAAGGGTACAGATTTCTTTGAGACTAGTGCAATGCTCATCTCATTCATTCTCTTAGGGAAGTACCTAGAGGTGTTAGCTAAGGGGAAGACATCTGATGCTATTGCCAAACTGATGGATTTGGCACCTGAAACAGCCACTTTGTTGACTCTAGACGAAGAAGGAAACGTGTTAGGTGAAGAAGAAATTGATGGTCGGTTGATACAAAAGAACGATGTCATCAAAATTATTCCAGGGGCGAAAGTAGCTTCGGATGGTTATGTGATATGGGGAAAAAGCCATGTAAATGAGAGTATGATAACAGGAGAAGCGCGTCCAGTGGCAAAAAGGAAGGGTGATATAGTCATTGGAGGTACTGTAAATGAAAATGGTGTGTTGCATATCAAGGCAACACTGGTTGGATCAGAAAGTTCCCTCGCGCAGATTGTATGGCTTGTTGAGTCAGCTCAGATGGCAAAAGCTCCTGTTCAGAAGTTTGCTGACCGCATTTCTAAATTCTTTGTGCCACTGGTGATCATGCTTTCATTCTTTACCTGGCTTTCATGGTTTTTGGCTGGAAAATTCCATGGCGACCCGAAATCTTGGATACCGAAGTCCATGGATAGCTTTCAGCTTGCTCTCCAGTTTGGGATCTCTGTCATGGTTATTGCGTGCCCTTGTGCTTTAGGCCTAGCAACTCCTACTGCTGTCATGGTTGGTACTGGAGTCGGTGCATCCCAAGGTGTACTGATCAAAGGAGGTCAAGCATTAGAAAGTGCATATAAGGTGGACTGCATTGTATTCGACAAGACAGGGACTCTTACAATTGGGAAGCCACTGGTTGTTAACACACGGCTCCTGAAAAATATGGTGCTTCGAGAATTCTATGAACTTGTTGCTGCAGCTGAGGTTAACAGTGAACACCCCTTGGCCAAGGCCATTGTTGACTATGCCAAAAAATTCAGAGAAGATGAAGAGAATCCAGCCGGGCCAGAAGTGCATGACTTTGCCTCCATTACTGGTCACGGGGTGAAGGCTATTGTTCGGGGCAGGGAAATAATTGTGGGGAACAAGAGCTTGATGGTGGACCAGAACATTGCAGTTCCAATCGTTGCAGAAGACTACCTAGCAGAAGCTGAAGGGCTGGCTCAAACTGGGATTCTAGCAGCCATAGATGGAGAAGTGGCCGGAGTTCTAGCAATATCTGATCCACTGAAACCAGGTGCTCAAGAAGTAATTACCATACTCAAGTCAATGAATGTTAGAAGCATAATGGTGACAGGTGACAATTGGGGAACTGCAAATTCTATTGCCAATGAAGTTGGTATTGATACTGTTATAGCCGAAGTGAAACCTGACCTGAAAGCAGAGCAAGTGAAGGAATTGCAGGCTTCAGGCTACACTGTGGCGATGGTGGGAGATGGCATCAATGACTCACCAGCACTAGCGGCGGCAGATGTAGGAATGGCAATTGGTGCTGGCACTGACATTGCAATTGAGGCAGCAGACATTGTGCTAATGAAGAGCAACTTGGAGGATGTGATAACTGCCATTGATCTTTCAAGGAAAACCTTCACTCATATTCGTTTGAACTACATTTGGTCTTTGGGATATAATGTGCTCGGGATCCCAATAGCTGCAGGAGTTCTCTTCCCATCCACTGGATTTCGCTTACCCCCTTGGATTGCTGGAGCTGCAATGGCTGCTTCTTCTGTCAGTGTTGTTTGCTGTTCATTGTTGTTGAAGAACTATAAGAGACCCAAGGAGCTGAATAACCTTCAGGTGCGTGGAATAGTGATCGAGTGCTCACTAATAGGCTTTTGGATCTTGCGTTTGTATGTATCTAGGAAGTTCTTAGGTAGTGTTTTGGCTGATGAAGCAAGTAATGTGCAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0008150 GO:0008324 GO:0009636 GO:0010035 GO:0010038 GO:0010273 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042221 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046688 GO:0046873 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0061687 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0097501 GO:0098655 GO:0098660 GO:0098662 GO:0098754 GO:0099131 GO:0099132 GO:1990169

KEGG Pathways

Metabolic & Signaling

ko01524 ko04016 map01524 map04016

Pfam Domains

Protein Families

E1-E2_ATPase HMA Hydrolase

Protein Analysis

1008

Amino Acids

108.75

Weight (kDa)

5.77

Isoelectric Point (pI)

27.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMA	PF00403	56 - 116	6.3e-14	Heavy-metal-associated domain
HMA	PF00403	137 - 192	7.9e-07	Heavy-metal-associated domain
E1-E2_ATPase	PF00122	424 - 530	6.1e-21	P-type ATPase actuator domain
Hydrolase	PF00702	639 - 862	7.6e-35	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002025)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G63440
fragaria_vesca	FvH4_5g02090 FvH4_5g02090 FvH4_5g02091 FvH4_5g02091 FvH4_5g02091 FvH4_5g02091
malus_domestica	MD06G1080800.v1.1 MD06G1080900.v1.1 MD14G1102600.v1.1
prunus_persica	Prupe.5G097900_v2.0.a1 Prupe.5G097900_v2.0.a1 Prupe.5G098000_v2.0.a1
pyrus_communis	pycom06g07780 pycom06g07790 pycom14g09890
rosa_chinensis	RchiOBHm_Chr7g0197781 RchiOBHm_Chr7g0198111 RchiOBHm_Chr7g0198141
rosa_laevigata	RLG00000003885 RLG00000003888 RLG00000019170
rosa_multiflora	Rmu_sc0000221.1_g000018 Rmu_sc0000221.1_g000030 Rmu_sc0006732.1_g000016
rosa_roxburghii	Rroxscaffold_3G00257940 Rroxscaffold_3G00257970 Rroxscaffold_3G00257990
rosa_rugosa	Rorug07G0045700 Rorug07G0045700 Rorug07G0045800 Rorug07G0045800 Rorug07G0045900
rosa_samantha	Rh7AG169100 Rh7AG169200 Rh7BG169900 Rh7BG172300 Rh7CG178600 Rh7CG178700 Rh7DG169200 Rh7DG171400 Rh7DG171800
rosa_wichuraiana	Rw7G014570 Rw7G014780 Rw7G014790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	974
Acc36I	ACCTGC	1 cut(s)	197
AccB1I	GGYRCC	2 cut(s)	149, 1273
AccB7I	CCANNNNNTGG	1 cut(s)	2789
AccI	GTMKAC	3 cut(s)	666, 989, 1116
AccII	CGCG	4 cut(s)	57, 79, 1462, 1568
AclI	AACGTT	1 cut(s)	624
AcoI	YGGCCR	2 cut(s)	2058, 2313
AcsI	RAATTY	8 cut(s)	690, 730, 828, 1643, 1709, 2009, 2087, 2431
AcuI	CTGAAG	4 cut(s)	516, 2286, 2505, 2891
AdeI	CACNNNGTG	1 cut(s)	2158
AfaI	GTAC	9 cut(s)	468, 839, 908, 1125, 1159, 1214, 1504, 1849, 1875
AfiI	CCNNNNNNNGG	2 cut(s)	794, 2789
AflII	CTTAAG	1 cut(s)	920
AflIII	ACRYGT	1 cut(s)	1317
AgeI	ACCGGT	1 cut(s)	208
AgsI	TTSAA	9 cut(s)	335, 350, 440, 560, 641, 749, 2691, 2719, 2872
AhlI	ACTAGT	1 cut(s)	1175
AjiI	CACGTC	1 cut(s)	262
AjnI	CCWGG	7 cut(s)	649, 761, 787, 913, 1381, 1682, 2347
AjuI	GAANNNNNNNTTGG	4 cut(s)	152, 184, 2642, 2674
AleI	CACNNNNGTG	2 cut(s)	140, 392
Alw21I	GWGCWC	4 cut(s)	267, 2356, 2751, 2936
Alw26I	GTCTC	4 cut(s)	308, 320, 1166, 2878
AlwNI	CAGNNNCTG	1 cut(s)	2264
Ama87I	CYCGRG	1 cut(s)	2750
AoxI	GGCC	8 cut(s)	80, 249, 722, 1816, 2058, 2064, 2117, 2313
ApaI	GGGCCC	1 cut(s)	726
ApoI	RAATTY	8 cut(s)	690, 730, 828, 1643, 1709, 2009, 2087, 2431
ArsI	GACNNNNNNTTYG	2 cut(s)	554, 586
AseI	ATTAAT	1 cut(s)	588
AsiGI	ACCGGT	1 cut(s)	208
Asp700I	GAANNNNTTC	2 cut(s)	691, 2699
AspLEI	GCGC	3 cut(s)	1050, 1462, 1570
AspS9I	GGNCC	5 cut(s)	206, 722, 723, 2117, 2215
AsuC2I	CCSGG	2 cut(s)	238, 2116
AsuII	TTCGAA	1 cut(s)	573
AvaI	CYCGRG	1 cut(s)	2750
AvaII	GGWCC	2 cut(s)	206, 2215
BaeGI	GKGCMC	4 cut(s)	152, 280, 726, 1805
BaeI	ACNNNNGTAYC	4 cut(s)	1727, 1760, 1839, 1872
BalI	TGGCCA	1 cut(s)	2060
BamHI	GGATCC	2 cut(s)	758, 2754
BanI	GGYRCC	2 cut(s)	149, 1273
BanII	GRGCYC	1 cut(s)	726
BauI	CACGAG	1 cut(s)	245
BbsI	GAAGAC	6 cut(s)	137, 567, 606, 892, 1244, 2253
Bbv12I	GWGCWC	4 cut(s)	267, 2356, 2751, 2936
BbvCI	CCTCAGC	1 cut(s)	2034
BceAI	ACGGC	2 cut(s)	236, 1997
BcgI	CGANNNNNNTGC	2 cut(s)	1912, 1946
BciT130I	CCWGG	7 cut(s)	651, 763, 789, 915, 1383, 1684, 2349
BciVI	GTATCC	2 cut(s)	117, 1728
BclI	TGATCA	2 cut(s)	1662, 1878
BcnI	CCSGG	2 cut(s)	238, 2116
BcoDI	GTCTC	4 cut(s)	308, 320, 1166, 2878
BcuI	ACTAGT	1 cut(s)	1175
BfaI	CTAG	9 cut(s)	1176, 1217, 1301, 1820, 2255, 2291, 2324, 2573, 2973
BfmI	CTRYAG	3 cut(s)	841, 2028, 2766
BfrI	CTTAAG	1 cut(s)	920
BfuAI	ACCTGC	1 cut(s)	197
BfuI	GTATCC	2 cut(s)	117, 1728
BglII	AGATCT	1 cut(s)	616
BlpI	GCTNAGC	1 cut(s)	956
BmcAI	AGTACT	1 cut(s)	839
Bme1390I	CCNGG	9 cut(s)	238, 651, 763, 789, 915, 1383, 1684, 2116, 2349
Bme18I	GGWCC	2 cut(s)	206, 2215
BmeT110I	CYCGRG	1 cut(s)	2750
BmgBI	CACGTC	1 cut(s)	262
BmgT120I	GGNCC	5 cut(s)	206, 722, 723, 2117, 2215
BmiI	GGNNCC	8 cut(s)	151, 207, 724, 725, 760, 1275, 1985, 2756
BmrFI	CCNGG	9 cut(s)	238, 651, 763, 789, 915, 1383, 1684, 2116, 2349
BmrI	ACTGGG	2 cut(s)	1038, 2292
BmsI	GCATC	4 cut(s)	328, 1237, 1871, 2562
BmuI	ACTGGG	2 cut(s)	1038, 2292
BpiI	GAAGAC	6 cut(s)	137, 567, 606, 892, 1244, 2253
BplI	GAGNNNNNCTC	2 cut(s)	837, 869
BpmI	CTGGAG	5 cut(s)	877, 1036, 1754, 1872, 2838
Bpu10I	CCTNAGC	3 cut(s)	487, 1230, 2034
Bpu1102I	GCTNAGC	1 cut(s)	956
Bpu14I	TTCGAA	1 cut(s)	573
BpuEI	CTTGAG	3 cut(s)	44, 2340, 2360
BpuMI	CCSGG	2 cut(s)	238, 2116
BsaI	GGTCTC	1 cut(s)	2878
BsaWI	WCCGGW	2 cut(s)	208, 994
BsaXI	ACNNNNNCTCC	6 cut(s)	77, 107, 1491, 1521, 2123, 2153
Bsc4I	CCNNNNNNNGG	2 cut(s)	794, 2789
Bse118I	RCCGGY	1 cut(s)	208
Bse3DI	GCAATG	5 cut(s)	423, 1188, 2223, 2616, 2832
BseBI	CCWGG	7 cut(s)	651, 763, 789, 915, 1383, 1684, 2349
BseGI	GGATG	6 cut(s)	909, 1075, 1408, 1862, 2671, 2783
BseLI	CCNNNNNNNGG	2 cut(s)	794, 2789
BseMI	GCAATG	5 cut(s)	423, 1188, 2223, 2616, 2832
BseMII	CTCAG	4 cut(s)	970, 1118, 1610, 2025
BseSI	GKGCMC	4 cut(s)	152, 280, 726, 1805
BsgI	GTGCAG	2 cut(s)	418, 1019
Bsh1236I	CGCG	4 cut(s)	57, 79, 1462, 1568
BshFI	GGCC	8 cut(s)	82, 251, 724, 1818, 2060, 2066, 2119, 2315
BshNI	GGYRCC	2 cut(s)	149, 1273
BshTI	ACCGGT	1 cut(s)	208
BsiHKAI	GWGCWC	4 cut(s)	267, 2356, 2751, 2936
BsiHKCI	CYCGRG	1 cut(s)	2750
BsiSI	CCGG	5 cut(s)	209, 237, 995, 2115, 2316
BslFI	GGGAC	2 cut(s)	219, 1957
BslI	CCNNNNNNNGG	2 cut(s)	794, 2789
BsmAI	GTCTC	4 cut(s)	308, 320, 1166, 2878
BsmFI	GGGAC	2 cut(s)	219, 1957
BsnI	GGCC	8 cut(s)	82, 251, 724, 1818, 2060, 2066, 2119, 2315
Bso31I	GGTCTC	1 cut(s)	2878
BsoBI	CYCGRG	1 cut(s)	2750
Bsp119I	TTCGAA	1 cut(s)	573
Bsp120I	GGGCCC	1 cut(s)	722
Bsp1286I	GDGCHC	8 cut(s)	152, 267, 280, 726, 1805, 2356, 2751, 2936
Bsp1407I	TGTACA	2 cut(s)	466, 906
Bsp1720I	GCTNAGC	1 cut(s)	956
Bsp19I	CCATGG	4 cut(s)	894, 1051, 1714, 1746
BspANI	GGCC	8 cut(s)	82, 251, 724, 1818, 2060, 2066, 2119, 2315
BspCNI	CTCAG	4 cut(s)	969, 1119, 1609, 2026
BspFNI	CGCG	4 cut(s)	57, 79, 1462, 1568
BspHI	TCATGA	1 cut(s)	676
BspLI	GGNNCC	8 cut(s)	151, 207, 724, 725, 760, 1275, 1985, 2756
BspMAI	CTGCAG	2 cut(s)	2032, 2770
BspMI	ACCTGC	1 cut(s)	197
BspQI	GCTCTTC	1 cut(s)	2645
BspT104I	TTCGAA	1 cut(s)	573
BspT107I	GGYRCC	2 cut(s)	149, 1273
BspTI	CTTAAG	1 cut(s)	920
BspTNI	GGTCTC	1 cut(s)	2878
BsrDI	GCAATG	5 cut(s)	423, 1188, 2223, 2616, 2832
BsrFI	RCCGGY	1 cut(s)	208
BsrGI	TGTACA	2 cut(s)	466, 906
BssAI	RCCGGY	1 cut(s)	208
BssSI	CACGAG	1 cut(s)	245
Bst2BI	CACGAG	1 cut(s)	245
Bst2UI	CCWGG	7 cut(s)	651, 763, 789, 915, 1383, 1684, 2349
Bst4CI	ACNGT	8 cut(s)	116, 275, 436, 566, 1507, 2045, 2464, 2533
Bst6I	CTCTTC	6 cut(s)	221, 642, 799, 2097, 2645, 2783
BstAFI	CTTAAG	1 cut(s)	920
BstAPI	GCANNNNNTGC	3 cut(s)	189, 2603, 2831
BstAUI	TGTACA	2 cut(s)	466, 906
BstBI	TTCGAA	1 cut(s)	573
BstC8I	GCNNGC	7 cut(s)	199, 453, 1764, 1801, 2274, 2517, 2608
BstDEI	CTNAG	9 cut(s)	487, 931, 956, 1127, 1204, 1230, 1596, 2034, 2983
BstDSI	CCRYGG	6 cut(s)	77, 252, 894, 1051, 1714, 1746
BstF5I	GGATG	6 cut(s)	909, 1075, 1408, 1862, 2671, 2783
BstFNI	CGCG	4 cut(s)	57, 79, 1462, 1568
BstHHI	GCGC	3 cut(s)	1050, 1462, 1570
BstMAI	GTCTC	4 cut(s)	308, 320, 1166, 2878
BstNI	CCWGG	7 cut(s)	651, 763, 789, 915, 1383, 1684, 2349
BstSCI	CCNGG	9 cut(s)	236, 649, 761, 787, 913, 1381, 1682, 2114, 2347
BstSFI	CTRYAG	3 cut(s)	841, 2028, 2766
BstSLI	GKGCMC	4 cut(s)	152, 280, 726, 1805
BstUI	CGCG	4 cut(s)	57, 79, 1462, 1568
BstV2I	GAAGAC	6 cut(s)	137, 567, 606, 892, 1244, 2253
BstX2I	RGATCY	5 cut(s)	616, 758, 1779, 2754, 2952
BstXI	CCANNNNNNTGG	2 cut(s)	142, 1264
BstYI	RGATCY	5 cut(s)	616, 758, 1779, 2754, 2952
BsuI	GTATCC	2 cut(s)	117, 1728
BsuRI	GGCC	8 cut(s)	82, 251, 724, 1818, 2060, 2066, 2119, 2315
BtgI	CCRYGG	6 cut(s)	77, 252, 894, 1051, 1714, 1746
BtgZI	GCGATG	1 cut(s)	2552
BtrI	CACGTC	1 cut(s)	262
BtsCI	GGATG	6 cut(s)	909, 1075, 1408, 1862, 2671, 2783
BtsI	GCAGTG	1 cut(s)	171
BveI	ACCTGC	1 cut(s)	197
Cac8I	GCNNGC	7 cut(s)	199, 453, 1764, 1801, 2274, 2517, 2608
CaiI	CAGNNNCTG	1 cut(s)	2264
CciI	TCATGA	1 cut(s)	676
CfoI	GCGC	3 cut(s)	1050, 1462, 1570
Cfr10I	RCCGGY	1 cut(s)	208
Cfr13I	GGNCC	5 cut(s)	206, 722, 723, 2117, 2215
Cfr42I	CCGCGG	1 cut(s)	80
CseI	GACGC	1 cut(s)	1451
CsiI	ACCWGGT	1 cut(s)	2347
Csp6I	GTAC	9 cut(s)	467, 838, 907, 1124, 1158, 1213, 1503, 1848, 1874
CspAI	ACCGGT	1 cut(s)	208
CviQI	GTAC	9 cut(s)	467, 838, 907, 1124, 1158, 1213, 1503, 1848, 1874
DdeI	CTNAG	9 cut(s)	487, 931, 956, 1127, 1204, 1230, 1596, 2034, 2983
DraIII	CACNNNGTG	1 cut(s)	2158
EaeI	YGGCCR	2 cut(s)	2058, 2313
Eam1104I	CTCTTC	6 cut(s)	221, 642, 799, 2097, 2645, 2783
EarI	CTCTTC	6 cut(s)	221, 642, 799, 2097, 2645, 2783
EciI	GGCGGA	2 cut(s)	140, 184
Eco147I	AGGCCT	1 cut(s)	1818
Eco24I	GRGCYC	1 cut(s)	726
Eco31I	GGTCTC	1 cut(s)	2878
Eco47I	GGWCC	2 cut(s)	206, 2215
Eco57I	CTGAAG	4 cut(s)	516, 2286, 2505, 2891
Eco88I	CYCGRG	1 cut(s)	2750
EcoO109I	RGGNCCY	2 cut(s)	722, 723
EcoRI	GAATTC	1 cut(s)	2009
EcoRII	CCWGG	7 cut(s)	649, 761, 787, 913, 1381, 1682, 2347
EcoT38I	GRGCYC	1 cut(s)	726
FalI	AAGNNNNNCTT	6 cut(s)	567, 599, 914, 946, 2642, 2674
FaqI	GGGAC	2 cut(s)	219, 1957
FbaI	TGATCA	2 cut(s)	1662, 1878
FblI	GTMKAC	3 cut(s)	666, 989, 1116
FokI	GGATG	6 cut(s)	896, 1082, 1415, 1849, 2678, 2770
FriOI	GRGCYC	1 cut(s)	726
FspBI	CTAG	9 cut(s)	1176, 1217, 1301, 1820, 2255, 2291, 2324, 2573, 2973
GlaI	GCGC	3 cut(s)	1049, 1461, 1569
GsuI	CTGGAG	5 cut(s)	877, 1036, 1754, 1872, 2838
HaeIII	GGCC	8 cut(s)	82, 251, 724, 1818, 2060, 2066, 2119, 2315
HapII	CCGG	5 cut(s)	209, 237, 995, 2115, 2316
HgaI	GACGC	1 cut(s)	1451
HhaI	GCGC	3 cut(s)	1050, 1462, 1570
Hin6I	GCGC	3 cut(s)	1048, 1460, 1568
HinP1I	GCGC	3 cut(s)	1048, 1460, 1568
HincII	GTYRAC	6 cut(s)	259, 307, 1296, 1975, 2041, 2074
HindII	GTYRAC	6 cut(s)	259, 307, 1296, 1975, 2041, 2074
HpaI	GTTAAC	3 cut(s)	307, 1975, 2041
HpaII	CCGG	5 cut(s)	209, 237, 995, 2115, 2316
Hpy188I	TCNGA	8 cut(s)	111, 1246, 1402, 1555, 1599, 1620, 2093, 2335
Hpy188III	TCNNGA	9 cut(s)	23, 245, 677, 856, 1301, 1987, 2006, 2357, 2752
HpyCH4III	ACNGT	8 cut(s)	116, 275, 436, 566, 1507, 2045, 2464, 2533
HpyCH4IV	ACGT	3 cut(s)	261, 624, 1317
HpyF3I	CTNAG	9 cut(s)	487, 931, 956, 1127, 1204, 1230, 1596, 2034, 2983
HpySE526I	ACGT	3 cut(s)	261, 624, 1317
HspAI	GCGC	3 cut(s)	1048, 1460, 1568
Ksp22I	TGATCA	2 cut(s)	1662, 1878
KspAI	GTTAAC	3 cut(s)	307, 1975, 2041
KspI	CCGCGG	1 cut(s)	80
LguI	GCTCTTC	1 cut(s)	2645
LmnI	GCTCC	7 cut(s)	197, 797, 967, 1615, 1989, 2819, 2893
LweI	GCATC	4 cut(s)	328, 1237, 1871, 2562
MabI	ACCWGGT	1 cut(s)	2347
MaeI	CTAG	9 cut(s)	1176, 1217, 1301, 1820, 2255, 2291, 2324, 2573, 2973
MaeII	ACGT	3 cut(s)	261, 624, 1317
MaeIII	GTNAC	3 cut(s)	2150, 2404, 2411
MfeI	CAATTG	4 cut(s)	1953, 2416, 2598, 2622
MflI	RGATCY	5 cut(s)	616, 758, 1779, 2754, 2952
MhlI	GDGCHC	8 cut(s)	152, 267, 280, 726, 1805, 2356, 2751, 2936
MlsI	TGGCCA	1 cut(s)	2060
MluNI	TGGCCA	1 cut(s)	2060
MlyI	GAGTC	6 cut(s)	855, 1291, 1598, 1863, 1939, 2555
MmeI	TCCRAC	1 cut(s)	1528
Mox20I	TGGCCA	1 cut(s)	2060
MroXI	GAANNNNTTC	2 cut(s)	691, 2699
MscI	TGGCCA	1 cut(s)	2060
MseI	TTAA	9 cut(s)	306, 588, 621, 887, 921, 1076, 1152, 1974, 2040
MslI	CAYNNNNRTG	7 cut(s)	140, 182, 392, 678, 740, 1434, 2402
Msp20I	TGGCCA	1 cut(s)	2060
MspA1I	CMGCKG	3 cut(s)	79, 544, 2033
MspCI	CTTAAG	1 cut(s)	920
MspI	CCGG	5 cut(s)	209, 237, 995, 2115, 2316
MspR9I	CCNGG	9 cut(s)	238, 651, 763, 789, 915, 1383, 1684, 2116, 2349
MunI	CAATTG	4 cut(s)	1953, 2416, 2598, 2622
MvaI	CCWGG	7 cut(s)	651, 763, 789, 915, 1383, 1684, 2349
MvnI	CGCG	4 cut(s)	57, 79, 1462, 1568
NciI	CCSGG	2 cut(s)	238, 2116
NcoI	CCATGG	4 cut(s)	894, 1051, 1714, 1746
NlaIV	GGNNCC	8 cut(s)	151, 207, 724, 725, 760, 1275, 1985, 2756
NmuCI	GTSAC	3 cut(s)	2150, 2404, 2411
NspV	TTCGAA	1 cut(s)	573
OliI	CACNNNNGTG	2 cut(s)	140, 392
PagI	TCATGA	1 cut(s)	676
PasI	CCCWGGG	1 cut(s)	650
PceI	AGGCCT	1 cut(s)	1818
PciSI	GCTCTTC	1 cut(s)	2645
PdmI	GAANNNNTTC	2 cut(s)	691, 2699
PfeI	GAWTC	7 cut(s)	155, 241, 440, 570, 982, 2107, 2287
PflMI	CCANNNNNTGG	1 cut(s)	2789
PinAI	ACCGGT	1 cut(s)	208
PleI	GAGTC	6 cut(s)	854, 1291, 1597, 1862, 1939, 2555
PpsI	GAGTC	6 cut(s)	854, 1291, 1597, 1862, 1939, 2555
PshBI	ATTAAT	1 cut(s)	588
PsiI	TTATAA	1 cut(s)	974
Psp1406I	AACGTT	1 cut(s)	624
Psp6I	CCWGG	7 cut(s)	649, 761, 787, 913, 1381, 1682, 2347
PspGI	CCWGG	7 cut(s)	649, 761, 787, 913, 1381, 1682, 2347
PspN4I	GGNNCC	8 cut(s)	151, 207, 724, 725, 760, 1275, 1985, 2756
PspOMI	GGGCCC	1 cut(s)	722
PspPI	GGNCC	5 cut(s)	206, 722, 723, 2117, 2215
PstI	CTGCAG	2 cut(s)	2032, 2770
PstNI	CAGNNNCTG	1 cut(s)	2264
PsuI	RGATCY	5 cut(s)	616, 758, 1779, 2754, 2952
PvuII	CAGCTG	2 cut(s)	544, 2033
RsaI	GTAC	9 cut(s)	468, 839, 908, 1125, 1159, 1214, 1504, 1849, 1875
RsaNI	GTAC	9 cut(s)	467, 838, 907, 1124, 1158, 1213, 1503, 1848, 1874
RseI	CAYNNNNRTG	7 cut(s)	140, 182, 392, 678, 740, 1434, 2402
SacII	CCGCGG	1 cut(s)	80
SapI	GCTCTTC	1 cut(s)	2645
SaqAI	TTAA	9 cut(s)	306, 588, 621, 887, 921, 1076, 1152, 1974, 2040
Sau96I	GGNCC	5 cut(s)	206, 722, 723, 2117, 2215
ScaI	AGTACT	1 cut(s)	839
SchI	GAGTC	6 cut(s)	855, 1291, 1598, 1863, 1939, 2555
ScrFI	CCNGG	9 cut(s)	238, 651, 763, 789, 915, 1383, 1684, 2116, 2349
SduI	GDGCHC	8 cut(s)	152, 267, 280, 726, 1805, 2356, 2751, 2936
SexAI	ACCWGGT	1 cut(s)	2347
SfaNI	GCATC	4 cut(s)	328, 1237, 1871, 2562
SfcI	CTRYAG	3 cut(s)	841, 2028, 2766
Sfr303I	CCGCGG	1 cut(s)	80
SfuI	TTCGAA	1 cut(s)	573
SgrBI	CCGCGG	1 cut(s)	80
SinI	GGWCC	2 cut(s)	206, 2215
SmiMI	CAYNNNNRTG	7 cut(s)	140, 182, 392, 678, 740, 1434, 2402
SmlI	CTYRAG	4 cut(s)	23, 920, 2355, 2375
SmoI	CTYRAG	4 cut(s)	23, 920, 2355, 2375
SpeI	ACTAGT	1 cut(s)	1175
SseBI	AGGCCT	1 cut(s)	1818
SspMI	CTAG	9 cut(s)	1176, 1217, 1301, 1820, 2255, 2291, 2324, 2573, 2973
StuI	AGGCCT	1 cut(s)	1818
StyD4I	CCNGG	9 cut(s)	236, 649, 761, 787, 913, 1381, 1682, 2114, 2347
TaaI	ACNGT	8 cut(s)	116, 275, 436, 566, 1507, 2045, 2464, 2533
TaiI	ACGT	3 cut(s)	264, 627, 1320
TaqI	TCGA	4 cut(s)	573, 1932, 2005, 2928
TatI	WGTACW	4 cut(s)	466, 837, 906, 1873
TauI	GCSGC	6 cut(s)	60, 63, 79, 82, 2579, 2582
TfiI	GAWTC	7 cut(s)	155, 241, 440, 570, 982, 2107, 2287
Tru1I	TTAA	9 cut(s)	306, 588, 621, 887, 921, 1076, 1152, 1974, 2040
Tru9I	TTAA	9 cut(s)	306, 588, 621, 887, 921, 1076, 1152, 1974, 2040
TseFI	GTSAC	3 cut(s)	2150, 2404, 2411
Tsp45I	GTSAC	3 cut(s)	2150, 2404, 2411
TspGWI	ACGGA	3 cut(s)	163, 311, 1111
Van91I	CCANNNNNTGG	1 cut(s)	2789
Vha464I	CTTAAG	1 cut(s)	920
VpaK11BI	GGWCC	2 cut(s)	206, 2215
VspI	ATTAAT	1 cut(s)	588
XapI	RAATTY	8 cut(s)	690, 730, 828, 1643, 1709, 2009, 2087, 2431
XbaI	TCTAGA	1 cut(s)	1300
XcmI	CCANNNNNNNNNTGG	1 cut(s)	142
XmiI	GTMKAC	3 cut(s)	666, 989, 1116
XmnI	GAANNNNTTC	2 cut(s)	691, 2699
XspI	CTAG	9 cut(s)	1176, 1217, 1301, 1820, 2255, 2291, 2324, 2573, 2973
ZrmI	AGTACT	1 cut(s)	839

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.