Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0002025

Physical Location & Seq

Genomic Coordinates Forward (+)

19896205 .. 19906716

Gene Structure 10512 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1080900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2940 bp

ATGGGTTGCATTTGCAATGACTGCTGGGGTGACATGTCACCCCGGCCGTGCTATCCGTCGATGTCTAAGAACCCGAAAGGTACATCGCCGGAAGCAAGGCATGTAAATGGCTCTGAGGCGAAGGCCTTGTTCTCTGTGGTTGGCATCACATGTTCTGCATGCGCTGGATCGATTGAAAAGGCGGTCAAGCGGCTTCCGGGCATCCGTGAGACTGCCGTTGAAGTGTTGAATAATTCAGTCCATGTTCTCTACTATCCAAGCTTGGTTACTGAGGAAAAAATATGCGAGACTATTGAAGATGCGGGATTTGAAGCCAAATTGATCAAGGAGGAGACAATTAATGGCAAATTAAGACAAGTATGCAGAATAAGCATAAATGGAATGACTTGCACCTCTTGCTCCTCCACTGTTGAATCTGCCCTCCAAGCAGTTCATGGTGTACAAAGAGCTCAAGTGGCCTTAGCAACTGAAGAAGCAGAAGTTCATTACGATCCAAAGGTTGTAAGCTACAATCAGCTGTTGGAAATCGTCGAAAACACTGGATTTGAAGCGAAACTTATTAGTCTAGGGGACGACATAAGCAAGATTGAGCTCAAAGTTGACGGCATAAAGACTGAACAAAGCATTAGAGCAATAACAAAATCCCTTGAAGCACTTCCTGGGGTTCAAGATATAGAGACATTCCCCGAACTCAACAAAATTTCGGTTTCTTATAAACAAGCTGTGATAGGACCAAGAACTTTCATTGAAGTGATCGAGTCATCAGGGTCCGCGCATTTCAAAGCAATGATATACCCTGAAGGGGGGAGAGACATTCATAGAAAGGAGGAAGTTAAGCAGTACTATAAATACTTTTTATGGAGTTTGTTTTTCGCAATTCCCGTGTTTTTAACCTCCATGGTGTTGATGTATATCCCTGGAATTAAGAAGGTGCTTGATGTTAGAATTGTGAATAAGTTGACAGTTGGTCAGATTCTGAGGTGGGAGCTGTCCACTCCAGTGCAGTTCATCATAGGCAGGAGGTTCTACATTGGATCATACAAAGCGTTGCGCAACGGTTCTGCTAATATGGATGTTTTGATTGCCTTGGGAACCAATGCAGCCTATTTCTATTCAGTCTATATAGTTTTAAGATCTGCAATCTCTAGAGATTTCAAGGGTACAGATTTCTTTGAGACTAGCTCAATGCTTATTACATTTATCTTGTTAGGCAAGTATCTAGAGGTTTTGGCAAAGGGGAAGACATCTGATGCCATTGCCAAGCTTATGAACTTGGCACCCGAGACAGCAACGTTGCTGAGTTTGGATGAGGATGGAAACGTGGTAAACGAACAAGAAATTGATAGTAGGTTGATACAAAAGAATGATGTGATTAAAATCATTCCAGGTGCTAAAGTTGCCTGTGATGGTTTTGTTGTATGGGGGCAGAGCCATGTGAATGAAAGCATGATAACAGGAGAAGCGCGGCCGGTGGGTAAAAATAAGGGTGATGCTGTCATTGGAGGCACTGTGAATGATAATGGAGTGTTGCATATTAAGGCAACAAGGGTTGGATCCGAGAGTGCTCTTTCGCAGATTGTTCGACTTGTTGAATCTGCGCAAATGGCCAAAGCTCCTGTACAGAAGTTTGCTGATTGCATTTCGAAATACTTTGTGCCAACGGTTATCATTCTTTCATTTTCGACGTGGCTTGCCTGGTTTTTATCAGGGAAGTTCCATAGCTACCCACATTCTTGGATACCATCTTCTATCGACAGTTTTGAGCTTGCGCTTCAGTTCGGGATCTCAGTCATGGTCATAGCTTGCCCTTGTGCCTTAGGCCTTGCAACTCCTACTGCCGTTATGGTTGGTACCGGAGTTGGTGCATCTCAAGGTATACTGATCAAAGGGGGCCAAGCATTAGAGAGCGCACATAAGGTGAACTGCATTGTTTTCGACAAGACGGGGACTCTCACAGTTGGGAAACCAGTGGTTGTAAACACAAAAATCTTCAATAATATGCTCCGTCATGAATTTTATGAACTTGTTGCAGCAACTGAGGTCAACAGTGAGCATCCACTAGCCAAGGCTATTGTAGAGTATGCCAAGAAATTCAGAGAAGGTGAAGACAATCCTTCCTGGCCCGAAGCACAAAATTTTGCATCCATTACTGGCCAAGGATTGAAGGCAGTTGTTCGGGACAAGGAGATAATCGTAGGCAACGAGAGTTTAATGTTGGATTCTAACATTGCCATTCCGGTGGAGGCTGAAGCTACACTAGCAGAAGCTGAAGCATTAGCTCAAACCGGGATTCTAATAGCTATAGACAGGGAAGTAGCAGGAGTTGTAGCCATATCCGATCCACTGAAACCCGGCGCCAAAGAAGTCGTTTCCATACTCAAGTCCATGGGAGTTAAAAGTATCATGGTTACAGGTGACAATTGGGGCACAGCCAATTCCATTGCCAAGGAAACTGAAATTGAAACCGTGATCGCAAAAGCCAAACCCGAACATAAAGCTGAGAAAGTGAAGGATTTGCAGGCTGAGGGATACGTTGTTGCAATGGTGGGAGATGGGATCAACGACTCACCGGCACTTGTGGCAGCAGATGTAGGAATGGCAATTGGTGCTGGCACAGATATTGCTATTGAAGCAGCTGACATTGTTCTTATGAAGAGCAACTTGGAAGATGTGATAACAGCCATTGACCTTTCCAGGAAAACCTTCTCTCGCATTCGTTTGAACTATATCTGGGCGTTGGGATATAATGTGCTCGGAATCCCAGTAGCTGCGGGAGCCCTTTTTCCATCCACCAGGTTTAGATTACCGCCATGGATTGCTGGAGCTGCAATGGCAGCCTCCTCTGTTAGCGTCGTTTGCTGCTCTTTGTTGTTGAAGAACTACAAGAGACCCAAGAAGTTGGATGTCCTTGAGATACATGAAGTAAGAATCGAACAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0008150 GO:0008324 GO:0009636 GO:0010035 GO:0010038 GO:0010273 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042221 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046688 GO:0046873 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0061687 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0097501 GO:0098655 GO:0098660 GO:0098662 GO:0098754 GO:0099131 GO:0099132 GO:1990169

KEGG Pathways

Metabolic & Signaling

ko01524 ko04016 map01524 map04016

Pfam Domains

Protein Families

E1-E2_ATPase HMA Hydrolase

Protein Analysis

980

Amino Acids

106.13

Weight (kDa)

6.04

Isoelectric Point (pI)

35.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMA	PF00403	44 - 104	8.2e-13	Heavy-metal-associated domain
HMA	PF00403	123 - 183	1.6e-14	Heavy-metal-associated domain
E1-E2_ATPase	PF00122	425 - 531	6.8e-25	P-type ATPase actuator domain
Hydrolase	PF00702	641 - 863	6.5e-35	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0002025)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G63440
fragaria_vesca	FvH4_5g02090 FvH4_5g02090 FvH4_5g02091 FvH4_5g02091 FvH4_5g02091 FvH4_5g02091
malus_domestica	MD06G1080800.v1.1 MD06G1080900.v1.1 MD14G1102600.v1.1
prunus_persica	Prupe.5G097900_v2.0.a1 Prupe.5G097900_v2.0.a1 Prupe.5G098000_v2.0.a1
pyrus_communis	pycom06g07780 pycom06g07790 pycom14g09890
rosa_chinensis	RchiOBHm_Chr7g0197781 RchiOBHm_Chr7g0198111 RchiOBHm_Chr7g0198141
rosa_laevigata	RLG00000003885 RLG00000003888 RLG00000019170
rosa_multiflora	Rmu_sc0000221.1_g000018 Rmu_sc0000221.1_g000030 Rmu_sc0006732.1_g000016
rosa_roxburghii	Rroxscaffold_3G00257940 Rroxscaffold_3G00257970 Rroxscaffold_3G00257990
rosa_rugosa	Rorug07G0045700 Rorug07G0045700 Rorug07G0045800 Rorug07G0045800 Rorug07G0045900
rosa_samantha	Rh7AG169100 Rh7AG169200 Rh7BG169900 Rh7BG172300 Rh7CG178600 Rh7CG178700 Rh7DG169200 Rh7DG171400 Rh7DG171800
rosa_wichuraiana	Rw7G014570 Rw7G014780 Rw7G014790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	714
Acc16I	TGCGCA	2 cut(s)	1052, 1599
Acc65I	GGTACC	1 cut(s)	1850
AccB1I	GGYRCC	3 cut(s)	1276, 1850, 2354
AccI	GTMKAC	1 cut(s)	1876
AccII	CGCG	2 cut(s)	773, 1465
AciI	CCGC	7 cut(s)	182, 190, 302, 771, 1465, 2771, 2807
AclI	AACGTT	1 cut(s)	1292
AclWI	GGATC	8 cut(s)	175, 485, 1042, 1548, 1561, 1790, 2333, 2564
AcoI	YGGCCR	4 cut(s)	44, 1466, 1605, 2152
AcsI	RAATTY	4 cut(s)	699, 2012, 2090, 2134
AcuI	CTGAAG	5 cut(s)	489, 819, 1757, 2268, 2289
AcyI	GRCGYC	1 cut(s)	2355
AfaI	GTAC	6 cut(s)	82, 441, 842, 1162, 1620, 1852
AfiI	CCNNNNNNNGG	2 cut(s)	802, 803
AflIII	ACRYGT	2 cut(s)	33, 149
AhdI	GACNNNNNGTC	1 cut(s)	966
AjiI	CACGTC	1 cut(s)	1686
AjnI	CCWGG	7 cut(s)	658, 916, 1384, 1694, 2117, 2693, 2792
AjuI	GAANNNNNNNTTGG	2 cut(s)	2645, 2677
AleI	CACNNNNGTG	1 cut(s)	998
Alw21I	GWGCWC	4 cut(s)	451, 594, 1567, 2754
Alw26I	GTCTC	8 cut(s)	203, 281, 326, 671, 804, 1169, 1277, 2881
AlwI	GGATC	8 cut(s)	175, 485, 1042, 1548, 1561, 1790, 2333, 2564
AlwNI	CAGNNNCTG	4 cut(s)	976, 2036, 2267, 2768
Ama87I	CYCGRG	1 cut(s)	1280
AoxI	GGCC	9 cut(s)	44, 123, 456, 1466, 1605, 1819, 1891, 2120, 2152
ApeKI	GCWGC	8 cut(s)	1100, 2030, 2582, 2633, 2768, 2825, 2834, 2859
ApoI	RAATTY	4 cut(s)	699, 2012, 2090, 2134
AseI	ATTAAT	1 cut(s)	339
Asp700I	GAANNNNTTC	2 cut(s)	654, 2702
Asp718I	GGTACC	1 cut(s)	1850
AspLEI	GCGC	8 cut(s)	164, 775, 1053, 1465, 1600, 1771, 1910, 2357
AspS9I	GGNCC	4 cut(s)	731, 768, 1891, 2121
AsuC2I	CCSGG	4 cut(s)	43, 198, 2287, 2352
AsuHPI	GGTGA	7 cut(s)	30, 41, 1499, 1930, 2114, 2426, 2559
AsuII	TTCGAA	1 cut(s)	1643
AvaI	CYCGRG	1 cut(s)	1280
AvaII	GGWCC	2 cut(s)	731, 768
AxyI	CCTNAGG	1 cut(s)	1816
BaeGI	GKGCMC	1 cut(s)	2429
BaeI	ACNNNNGTAYC	2 cut(s)	1842, 1875
BalI	TGGCCA	2 cut(s)	1607, 2154
BamHI	GGATCC	1 cut(s)	1553
BanI	GGYRCC	3 cut(s)	1276, 1850, 2354
BanII	GRGCYC	3 cut(s)	451, 594, 2779
BbsI	GAAGAC	2 cut(s)	1247, 2112
Bbv12I	GWGCWC	4 cut(s)	451, 594, 1567, 2754
BbvCI	CCTCAGC	1 cut(s)	2523
BbvI	GCAGC	8 cut(s)	1112, 2042, 2594, 2645, 2755, 2812, 2846, 2846
BccI	CCATC	5 cut(s)	1307, 1400, 1750, 2546, 2794
BceAI	ACGGC	4 cut(s)	31, 200, 619, 1823
BcgI	CGANNNNNNTGC	4 cut(s)	1562, 1596, 1915, 1949
BciT130I	CCWGG	7 cut(s)	660, 918, 1386, 1696, 2119, 2695, 2794
BciVI	GTATCC	2 cut(s)	1731, 2522
BclI	TGATCA	2 cut(s)	321, 1881
BcnI	CCSGG	4 cut(s)	43, 198, 2287, 2352
BcoDI	GTCTC	8 cut(s)	203, 281, 326, 671, 804, 1169, 1277, 2881
BfaI	CTAG	6 cut(s)	566, 1146, 1179, 1220, 2060, 2258
BfmI	CTRYAG	1 cut(s)	2301
BfoI	RGCGCY	1 cut(s)	2358
BfuI	GTATCC	2 cut(s)	1731, 2522
BglII	AGATCT	1 cut(s)	1133
BmcAI	AGTACT	1 cut(s)	842
Bme18I	GGWCC	2 cut(s)	731, 768
BmeRI	GACNNNNNGTC	1 cut(s)	966
BmeT110I	CYCGRG	1 cut(s)	1280
BmgBI	CACGTC	1 cut(s)	1686
BmgT120I	GGNCC	4 cut(s)	731, 768, 1891, 2121
BmiI	GGNNCC	8 cut(s)	769, 1093, 1278, 1555, 1852, 1892, 2356, 2776
BmrI	ACTGGG	1 cut(s)	2756
BmsI	GCATC	8 cut(s)	153, 210, 289, 1240, 1480, 1874, 2062, 2150
BmuI	ACTGGG	1 cut(s)	2756
BpiI	GAAGAC	2 cut(s)	1247, 2112
BplI	GAGNNNNNCTC	2 cut(s)	1166, 1198
BpmI	CTGGAG	2 cut(s)	981, 2841
Bpu10I	CCTNAGC	2 cut(s)	460, 2523
Bpu14I	TTCGAA	1 cut(s)	1643
BpuEI	CTTGAG	4 cut(s)	435, 1854, 2363, 2930
BpuMI	CCSGG	4 cut(s)	43, 198, 2287, 2352
Bsa29I	ATCGAT	1 cut(s)	170
BsaBI	GATNNNNATC	2 cut(s)	911, 1376
BsaHI	GRCGYC	1 cut(s)	2355
BsaI	GGTCTC	1 cut(s)	2881
BsaWI	WCCGGW	2 cut(s)	1853, 2236
BsaXI	ACNNNNNCTCC	2 cut(s)	1494, 1524
Bsc4I	CCNNNNNNNGG	2 cut(s)	802, 803
Bse118I	RCCGGY	2 cut(s)	1468, 2569
Bse1I	ACTGG	5 cut(s)	544, 998, 1967, 2155, 2762
Bse21I	CCTNAGG	1 cut(s)	1816
Bse3DI	GCAATG	7 cut(s)	22, 792, 1254, 2226, 2439, 2547, 2835
Bse8I	GATNNNNATC	2 cut(s)	911, 1376
BseBI	CCWGG	7 cut(s)	660, 918, 1386, 1696, 2119, 2695, 2794
BseCI	ATCGAT	1 cut(s)	170
BseGI	GGATG	8 cut(s)	201, 1078, 1312, 1318, 2053, 2141, 2786, 2908
BseJI	GATNNNNATC	2 cut(s)	911, 1376
BseLI	CCNNNNNNNGG	2 cut(s)	802, 803
BseMI	GCAATG	7 cut(s)	22, 792, 1254, 2226, 2439, 2547, 2835
BseMII	CTCAG	8 cut(s)	105, 261, 968, 1289, 1800, 2028, 2490, 2514
BseNI	ACTGG	5 cut(s)	544, 998, 1967, 2155, 2762
BseRI	GAGGAG	3 cut(s)	344, 391, 2830
BseSI	GKGCMC	1 cut(s)	2429
BseX3I	CGGCCG	2 cut(s)	44, 1466
BseXI	GCAGC	8 cut(s)	1112, 2042, 2594, 2645, 2755, 2812, 2846, 2846
BseYI	CCCAGC	1 cut(s)	24
BsgI	GTGCAG	1 cut(s)	1022
Bsh1236I	CGCG	2 cut(s)	773, 1465
Bsh1285I	CGRYCG	2 cut(s)	47, 1469
BshFI	GGCC	9 cut(s)	46, 125, 458, 1468, 1607, 1821, 1893, 2122, 2154
BshNI	GGYRCC	3 cut(s)	1276, 1850, 2354
BshVI	ATCGAT	1 cut(s)	170
BsiEI	CGRYCG	2 cut(s)	47, 1469
BsiHKAI	GWGCWC	4 cut(s)	451, 594, 1567, 2754
BsiHKCI	CYCGRG	1 cut(s)	1280
BsiSI	CCGG	9 cut(s)	43, 89, 197, 1469, 1854, 2237, 2286, 2352, 2570
BslFI	GGGAC	3 cut(s)	584, 1960, 2192
BslI	CCNNNNNNNGG	2 cut(s)	802, 803
BsmAI	GTCTC	8 cut(s)	203, 281, 326, 671, 804, 1169, 1277, 2881
BsmFI	GGGAC	3 cut(s)	584, 1960, 2192
BsmI	GAATGC	1 cut(s)	2712
BsnI	GGCC	9 cut(s)	46, 125, 458, 1468, 1607, 1821, 1893, 2122, 2154
Bso31I	GGTCTC	1 cut(s)	2881
BsoBI	CYCGRG	1 cut(s)	1280
Bsp119I	TTCGAA	1 cut(s)	1643
Bsp1286I	GDGCHC	6 cut(s)	451, 594, 1567, 2429, 2754, 2779
Bsp1407I	TGTACA	2 cut(s)	439, 1618
Bsp19I	CCATGG	3 cut(s)	897, 2385, 2810
BspACI	CCGC	7 cut(s)	182, 190, 302, 771, 1465, 2771, 2807
BspANI	GGCC	9 cut(s)	46, 125, 458, 1468, 1607, 1821, 1893, 2122, 2154
BspCNI	CTCAG	8 cut(s)	106, 262, 969, 1290, 1799, 2029, 2491, 2515
BspDI	ATCGAT	1 cut(s)	170
BspFNI	CGCG	2 cut(s)	773, 1465
BspHI	TCATGA	1 cut(s)	2008
BspLI	GGNNCC	8 cut(s)	769, 1093, 1278, 1555, 1852, 1892, 2356, 2776
BspPI	GGATC	8 cut(s)	175, 485, 1042, 1548, 1561, 1790, 2333, 2564
BspQI	GCTCTTC	1 cut(s)	2648
BspT104I	TTCGAA	1 cut(s)	1643
BspT107I	GGYRCC	3 cut(s)	1276, 1850, 2354
BspTNI	GGTCTC	1 cut(s)	2881
BsrDI	GCAATG	7 cut(s)	22, 792, 1254, 2226, 2439, 2547, 2835
BsrFI	RCCGGY	2 cut(s)	1468, 2569
BsrGI	TGTACA	2 cut(s)	439, 1618
BsrI	ACTGG	5 cut(s)	544, 998, 1967, 2155, 2762
BssAI	RCCGGY	2 cut(s)	1468, 2569
BssNAI	GTATAC	1 cut(s)	1877
BssNI	GRCGYC	1 cut(s)	2355
BssT1I	CCWWGG	7 cut(s)	897, 1086, 2064, 2155, 2385, 2445, 2810
Bst1107I	GTATAC	1 cut(s)	1877
Bst2UI	CCWGG	7 cut(s)	660, 918, 1386, 1696, 2119, 2695, 2794
Bst4CI	ACNGT	9 cut(s)	409, 964, 1058, 1510, 1663, 1757, 1957, 2048, 2467
Bst6I	CTCTTC	1 cut(s)	2648
BstACI	GRCGYC	1 cut(s)	2355
BstAPI	GCANNNNNTGC	3 cut(s)	21, 396, 2606
BstAUI	TGTACA	2 cut(s)	439, 1618
BstBI	TTCGAA	1 cut(s)	1643
BstC8I	GCNNGC	6 cut(s)	160, 1692, 1767, 1804, 2520, 2611
BstDSI	CCRYGG	3 cut(s)	897, 2385, 2810
BstF5I	GGATG	8 cut(s)	201, 1078, 1312, 1318, 2053, 2141, 2786, 2908
BstFNI	CGCG	2 cut(s)	773, 1465
BstH2I	RGCGCY	1 cut(s)	2358
BstHHI	GCGC	8 cut(s)	164, 775, 1053, 1465, 1600, 1771, 1910, 2357
BstMAI	GTCTC	8 cut(s)	203, 281, 326, 671, 804, 1169, 1277, 2881
BstMCI	CGRYCG	2 cut(s)	47, 1469
BstNI	CCWGG	7 cut(s)	660, 918, 1386, 1696, 2119, 2695, 2794
BstNSI	RCATGY	4 cut(s)	37, 104, 153, 162
BstSFI	CTRYAG	1 cut(s)	2301
BstSLI	GKGCMC	1 cut(s)	2429
BstUI	CGCG	2 cut(s)	773, 1465
BstV1I	GCAGC	8 cut(s)	1112, 2042, 2594, 2645, 2755, 2812, 2846, 2846
BstV2I	GAAGAC	2 cut(s)	1247, 2112
BstX2I	RGATCY	3 cut(s)	1133, 1553, 1782
BstXI	CCANNNNNNTGG	3 cut(s)	1734, 2239, 2899
BstYI	RGATCY	3 cut(s)	1133, 1553, 1782
BstZ17I	GTATAC	1 cut(s)	1877
BstZI	CGGCCG	2 cut(s)	44, 1466
Bsu15I	ATCGAT	1 cut(s)	170
Bsu36I	CCTNAGG	1 cut(s)	1816
BsuI	GTATCC	2 cut(s)	1731, 2522
BsuRI	GGCC	9 cut(s)	46, 125, 458, 1468, 1607, 1821, 1893, 2122, 2154
BsuTUI	ATCGAT	1 cut(s)	170
BtgI	CCRYGG	3 cut(s)	897, 2385, 2810
BtgZI	GCGATG	1 cut(s)	69
BtrI	CACGTC	1 cut(s)	1686
BtsCI	GGATG	8 cut(s)	201, 1078, 1312, 1318, 2053, 2141, 2786, 2908
BtsIMutI	CAGTG	7 cut(s)	405, 537, 1005, 1506, 1974, 2053, 2342
Cac8I	GCNNGC	6 cut(s)	160, 1692, 1767, 1804, 2520, 2611
CaiI	CAGNNNCTG	4 cut(s)	976, 2036, 2267, 2768
CciI	TCATGA	1 cut(s)	2008
CfoI	GCGC	8 cut(s)	164, 775, 1053, 1465, 1600, 1771, 1910, 2357
Cfr10I	RCCGGY	2 cut(s)	1468, 2569
Cfr13I	GGNCC	4 cut(s)	731, 768, 1891, 2121
ClaI	ATCGAT	1 cut(s)	170
CseI	GACGC	1 cut(s)	2839
CsiI	ACCWGGT	1 cut(s)	2792
Csp6I	GTAC	6 cut(s)	81, 440, 841, 1161, 1619, 1851
CviQI	GTAC	6 cut(s)	81, 440, 841, 1161, 1619, 1851
DinI	GGCGCC	1 cut(s)	2356
DriI	GACNNNNNGTC	1 cut(s)	966
EaeI	YGGCCR	4 cut(s)	44, 1466, 1605, 2152
EagI	CGGCCG	2 cut(s)	44, 1466
Eam1104I	CTCTTC	1 cut(s)	2648
Eam1105I	GACNNNNNGTC	1 cut(s)	966
EarI	CTCTTC	1 cut(s)	2648
Ecl136II	GAGCTC	2 cut(s)	449, 592
EclXI	CGGCCG	2 cut(s)	44, 1466
Eco130I	CCWWGG	7 cut(s)	897, 1086, 2064, 2155, 2385, 2445, 2810
Eco147I	AGGCCT	2 cut(s)	125, 1821
Eco24I	GRGCYC	3 cut(s)	451, 594, 2779
Eco31I	GGTCTC	1 cut(s)	2881
Eco47I	GGWCC	2 cut(s)	731, 768
Eco52I	CGGCCG	2 cut(s)	44, 1466
Eco53kI	GAGCTC	2 cut(s)	449, 592
Eco57I	CTGAAG	5 cut(s)	489, 819, 1757, 2268, 2289
Eco81I	CCTNAGG	1 cut(s)	1816
Eco88I	CYCGRG	1 cut(s)	1280
EcoICRI	GAGCTC	2 cut(s)	449, 592
EcoRII	CCWGG	7 cut(s)	658, 916, 1384, 1694, 2117, 2693, 2792
EcoT14I	CCWWGG	7 cut(s)	897, 1086, 2064, 2155, 2385, 2445, 2810
EcoT38I	GRGCYC	3 cut(s)	451, 594, 2779
EgeI	GGCGCC	1 cut(s)	2356
EheI	GGCGCC	1 cut(s)	2356
ErhI	CCWWGG	7 cut(s)	897, 1086, 2064, 2155, 2385, 2445, 2810
FalI	AAGNNNNNCTT	4 cut(s)	540, 572, 2645, 2677
FaqI	GGGAC	3 cut(s)	584, 1960, 2192
FauI	CCCGC	2 cut(s)	295, 2764
FbaI	TGATCA	2 cut(s)	321, 1881
FblI	GTMKAC	1 cut(s)	1876
FokI	GGATG	8 cut(s)	188, 1085, 1319, 1325, 2040, 2128, 2773, 2915
FriOI	GRGCYC	3 cut(s)	451, 594, 2779
FspBI	CTAG	6 cut(s)	566, 1146, 1179, 1220, 2060, 2258
FspI	TGCGCA	2 cut(s)	1052, 1599
GlaI	GCGC	8 cut(s)	163, 774, 1052, 1464, 1599, 1770, 1909, 2356
GsaI	CCCAGC	1 cut(s)	28
GsuI	CTGGAG	2 cut(s)	981, 2841
HaeII	RGCGCY	1 cut(s)	2358
HaeIII	GGCC	9 cut(s)	46, 125, 458, 1468, 1607, 1821, 1893, 2122, 2154
HapII	CCGG	9 cut(s)	43, 89, 197, 1469, 1854, 2237, 2286, 2352, 2570
HgaI	GACGC	1 cut(s)	2839
HhaI	GCGC	8 cut(s)	164, 775, 1053, 1465, 1600, 1771, 1910, 2357
Hin1I	GRCGYC	1 cut(s)	2355
Hin6I	GCGC	8 cut(s)	162, 773, 1051, 1463, 1598, 1769, 1908, 2355
HinP1I	GCGC	8 cut(s)	162, 773, 1051, 1463, 1598, 1769, 1908, 2355
HincII	GTYRAC	3 cut(s)	601, 960, 2044
HindII	GTYRAC	3 cut(s)	601, 960, 2044
HindIII	AAGCTT	2 cut(s)	259, 1262
HpaII	CCGG	9 cut(s)	43, 89, 197, 1469, 1854, 2237, 2286, 2352, 2570
HphI	GGTGA	7 cut(s)	30, 41, 1499, 1930, 2114, 2426, 2559
Hpy166II	GTNNAC	9 cut(s)	440, 601, 960, 993, 1327, 1877, 1921, 1978, 2044
Hpy188I	TCNGA	8 cut(s)	115, 972, 978, 1249, 1558, 2096, 2338, 2756
Hpy188III	TCNNGA	6 cut(s)	668, 1146, 1220, 1780, 2009, 2177
Hpy8I	GTNNAC	9 cut(s)	440, 601, 960, 993, 1327, 1877, 1921, 1978, 2044
Hpy99I	CGWCG	4 cut(s)	61, 533, 1687, 2855
HpyAV	CCTTC	8 cut(s)	115, 794, 922, 2093, 2124, 2158, 2503, 2713
HpyCH4III	ACNGT	9 cut(s)	409, 964, 1058, 1510, 1663, 1757, 1957, 2048, 2467
HpyCH4IV	ACGT	4 cut(s)	1292, 1320, 1685, 2532
HpySE526I	ACGT	4 cut(s)	1292, 1320, 1685, 2532
Hsp92I	GRCGYC	1 cut(s)	2355
HspAI	GCGC	8 cut(s)	162, 773, 1051, 1463, 1598, 1769, 1908, 2355
KasI	GGCGCC	1 cut(s)	2354
KpnI	GGTACC	1 cut(s)	1854
Ksp22I	TGATCA	2 cut(s)	321, 1881
LguI	GCTCTTC	1 cut(s)	2648
LmnI	GCTCC	6 cut(s)	404, 985, 1618, 2007, 2774, 2822
Lsp1109I	GCAGC	8 cut(s)	1112, 2042, 2594, 2645, 2755, 2812, 2846, 2846
LweI	GCATC	8 cut(s)	153, 210, 289, 1240, 1480, 1874, 2062, 2150
MabI	ACCWGGT	1 cut(s)	2792
MaeI	CTAG	6 cut(s)	566, 1146, 1179, 1220, 2060, 2258
MaeII	ACGT	4 cut(s)	1292, 1320, 1685, 2532
MaeIII	GTNAC	5 cut(s)	29, 36, 265, 2407, 2414
MboII	GAAGA	9 cut(s)	308, 482, 1252, 1737, 1981, 2117, 2665, 2678, 2887
MfeI	CAATTG	2 cut(s)	2419, 2601
MflI	RGATCY	3 cut(s)	1133, 1553, 1782
MhlI	GDGCHC	6 cut(s)	451, 594, 1567, 2429, 2754, 2779
MlsI	TGGCCA	2 cut(s)	1607, 2154
MluNI	TGGCCA	2 cut(s)	1607, 2154
Mly113I	GGCGCC	1 cut(s)	2355
MlyI	GAGTC	3 cut(s)	767, 1942, 2558
MmeI	TCCRAC	4 cut(s)	501, 1531, 2196, 2880
Mox20I	TGGCCA	2 cut(s)	1607, 2154
MroXI	GAANNNNTTC	2 cut(s)	654, 2702
MscI	TGGCCA	2 cut(s)	1607, 2154
MslI	CAYNNNNRTG	5 cut(s)	105, 749, 998, 1437, 2237
Msp20I	TGGCCA	2 cut(s)	1607, 2154
MspA1I	CMGCKG	2 cut(s)	517, 2636
MspI	CCGG	9 cut(s)	43, 89, 197, 1469, 1854, 2237, 2286, 2352, 2570
MunI	CAATTG	2 cut(s)	2419, 2601
Mva1269I	GAATGC	1 cut(s)	2712
MvaI	CCWGG	7 cut(s)	660, 918, 1386, 1696, 2119, 2695, 2794
MvnI	CGCG	2 cut(s)	773, 1465
NarI	GGCGCC	1 cut(s)	2355
NciI	CCSGG	4 cut(s)	43, 198, 2287, 2352
NcoI	CCATGG	3 cut(s)	897, 2385, 2810
NlaIV	GGNNCC	8 cut(s)	769, 1093, 1278, 1555, 1852, 1892, 2356, 2776
NmuCI	GTSAC	3 cut(s)	29, 36, 2414
NsbI	TGCGCA	2 cut(s)	1052, 1599
NspI	RCATGY	4 cut(s)	37, 104, 153, 162
NspV	TTCGAA	1 cut(s)	1643
OliI	CACNNNNGTG	1 cut(s)	998
PaeI	GCATGC	1 cut(s)	162
PagI	TCATGA	1 cut(s)	2008
PceI	AGGCCT	2 cut(s)	125, 1821
PciI	ACATGT	2 cut(s)	33, 149
PciSI	GCTCTTC	1 cut(s)	2648
PcsI	WCGNNNNNNNCGW	3 cut(s)	879, 1326, 2199
PctI	GAATGC	1 cut(s)	2712
PdmI	GAANNNNTTC	2 cut(s)	654, 2702
PfeI	GAWTC	7 cut(s)	413, 973, 1592, 2219, 2290, 2757, 2928
PfoI	TCCNGGA	1 cut(s)	2693
PleI	GAGTC	3 cut(s)	766, 1942, 2558
PluTI	GGCGCC	1 cut(s)	2358
PpsI	GAGTC	3 cut(s)	766, 1942, 2558
PscI	ACATGT	2 cut(s)	33, 149
PshBI	ATTAAT	1 cut(s)	339
PsiI	TTATAA	1 cut(s)	714
Psp124BI	GAGCTC	2 cut(s)	451, 594
Psp1406I	AACGTT	1 cut(s)	1292
Psp6I	CCWGG	7 cut(s)	658, 916, 1384, 1694, 2117, 2693, 2792
PspFI	CCCAGC	1 cut(s)	24
PspGI	CCWGG	7 cut(s)	658, 916, 1384, 1694, 2117, 2693, 2792
PspN4I	GGNNCC	8 cut(s)	769, 1093, 1278, 1555, 1852, 1892, 2356, 2776
PspPI	GGNCC	4 cut(s)	731, 768, 1891, 2121
PstNI	CAGNNNCTG	4 cut(s)	976, 2036, 2267, 2768
PsuI	RGATCY	3 cut(s)	1133, 1553, 1782
PvuII	CAGCTG	2 cut(s)	517, 2636
RsaI	GTAC	6 cut(s)	82, 441, 842, 1162, 1620, 1852
RsaNI	GTAC	6 cut(s)	81, 440, 841, 1161, 1619, 1851
RseI	CAYNNNNRTG	5 cut(s)	105, 749, 998, 1437, 2237
SacI	GAGCTC	2 cut(s)	451, 594
SapI	GCTCTTC	1 cut(s)	2648
Sau96I	GGNCC	4 cut(s)	731, 768, 1891, 2121
ScaI	AGTACT	1 cut(s)	842
SchI	GAGTC	3 cut(s)	767, 1942, 2558
SduI	GDGCHC	6 cut(s)	451, 594, 1567, 2429, 2754, 2779
SexAI	ACCWGGT	1 cut(s)	2792
SfaNI	GCATC	8 cut(s)	153, 210, 289, 1240, 1480, 1874, 2062, 2150
SfcI	CTRYAG	1 cut(s)	2301
SfoI	GGCGCC	1 cut(s)	2356
SfuI	TTCGAA	1 cut(s)	1643
SinI	GGWCC	2 cut(s)	731, 768
SmiMI	CAYNNNNRTG	5 cut(s)	105, 749, 998, 1437, 2237
SmlI	CTYRAG	4 cut(s)	450, 1869, 2378, 2909
SmoI	CTYRAG	4 cut(s)	450, 1869, 2378, 2909
SphI	GCATGC	1 cut(s)	162
SseBI	AGGCCT	2 cut(s)	125, 1821
SsiI	CCGC	7 cut(s)	182, 190, 302, 771, 1465, 2771, 2807
SspDI	GGCGCC	1 cut(s)	2354
SspMI	CTAG	6 cut(s)	566, 1146, 1179, 1220, 2060, 2258
SstI	GAGCTC	2 cut(s)	451, 594
StuI	AGGCCT	2 cut(s)	125, 1821
StyI	CCWWGG	7 cut(s)	897, 1086, 2064, 2155, 2385, 2445, 2810
TaaI	ACNGT	9 cut(s)	409, 964, 1058, 1510, 1663, 1757, 1957, 2048, 2467
TaiI	ACGT	4 cut(s)	1295, 1323, 1688, 2535
TatI	WGTACW	3 cut(s)	439, 840, 1618
TauI	GCSGC	2 cut(s)	193, 1468
TfiI	GAWTC	7 cut(s)	413, 973, 1592, 2219, 2290, 2757, 2928
TscAI	CASTG	7 cut(s)	412, 544, 1005, 1513, 1974, 2053, 2349
TseFI	GTSAC	3 cut(s)	29, 36, 2414
TseI	GCWGC	8 cut(s)	1100, 2030, 2582, 2633, 2768, 2825, 2834, 2859
Tsp45I	GTSAC	3 cut(s)	29, 36, 2414
TspGWI	ACGGA	3 cut(s)	45, 194, 1994
TspRI	CASTG	7 cut(s)	412, 544, 1005, 1513, 1974, 2053, 2349
VpaK11BI	GGWCC	2 cut(s)	731, 768
VspI	ATTAAT	1 cut(s)	339
XapI	RAATTY	4 cut(s)	699, 2012, 2090, 2134
XbaI	TCTAGA	2 cut(s)	1145, 1219
XceI	RCATGY	4 cut(s)	37, 104, 153, 162
XcmI	CCANNNNNNNNNTGG	1 cut(s)	431
XmiI	GTMKAC	1 cut(s)	1876
XmnI	GAANNNNTTC	2 cut(s)	654, 2702
XspI	CTAG	6 cut(s)	566, 1146, 1179, 1220, 2060, 2258
ZrmI	AGTACT	1 cut(s)	842

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.