Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0002025

Physical Location & Seq

Genomic Coordinates Forward (+)

52252289 .. 52257951

Gene Structure 5663 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00257940.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2928 bp

ATGTCGCCACGGCCTCACTACCCCTCCATGCCAAAATATCCGAAGGGTGTGGTGGCGGAGGAGACTACTATGGTGGTGGGCACCGAGTCCAAGGCCGTGTTCTCCGTCATCGGCATGACCTGCTCCGCCTGCGCGGGATCGGTAGAGAAGGCTGTAAAGAGGCTACCCGGGATTCGTGAGGCGGTGGTCGACGTGCTCAACAACCGTGCCCAAGTCATGTTCTTCCCCAATTTCGTTAACGCAGAGACTATCCGTGAGACTATTGAAGATGTTGGATTTCAAGCAACATTGATTGCTAATGAGGGAAACGAGAAGTCCACGTTGGTGTGCAGAATAAGGATCAAAGGAATGACTTGCACTTCTTGCTCTTCCACTGTTGAATCAGCTTTGCAGGCAGTTCATGGTGTGCAAAGAGCCCAAGTTGCCTTAGCAACTGAAGAAGCAGATGTCCACTATGATCCAAAGATAGTGAGCTGCAATCAGCTGATGGTAGCCATTGAAGACACTGGATTTGAAGCCATACTTATTAATTCAGGTGAAGACATGAGCAAGATAGATCTTAAAGTTGACGGTGTAAGGACTGATCATTCAATGAGAATACTTGAACAGTCTCTTCAAGCACTCCCTGGGGTGCAAGGTGTAGACATACATCATGATGACAGAAAAATTTCCCTGTCTTACAAGCCAGATATGACAGGGCCCCGAAGTTTCATCAATGTGATTGAAACAACTGGATCCAGGCGTTTCAGGGCAAAAATTTATCCAGGCGGTGGAGCAGGAAGAGAAAATCATAGGAAGGAGGAAATTCAGCAGTACTACAGAGTCTTTCTCTGGAGTTTGATTTTCACCATTCCAGTATTTTTAACCTCCATGGTCCTCATGTACATCCCTGGACTTAAGCATGGCTTAGACAAGAAAGTACTAAATATGCTCAGCATTGGGGAGCTTATAAGGTGGATTCTGTCTACTCCGGTGCAGTTCATCATAGGCCGGAGGTTCTATACTGGGGCATGCAAGTCATTGCGCCATGGTTCTGCTAATATGGATGTGTTAATTGCCTTGGGAACAAATGCAGCATATTTCTATTCAGTCTACTCCGTACTGAGAGCTGCCACCTCCCCACATTTTAAGGGTACAGATTTCTTTGAGACTAGTGCAATGCTCATCTCATTCATTCTTTTAGGGAAATACCTAGAGGTGTTAGCTAAGGGAAAGACATCTGATGCTATTGCCAAACTGATGGATTTGGCACCTGAAACAGCCACTTTGTTGACTCTAAACGAAGAAGGAAACGTGTTAGGTGAAGGAGAAATTGATGGTCGGTTGATACAAAAGAACGATGTCATCAAAATTATTCCAGGGGCGAAAGTAGCTTCGGATGGTTATGTGATATGGGGACAAAGCCATGTAAATGAGAGTATGATAACAGGAGAAGCGCGTCCAGTGGCAAAAAGGAAGGGTGATATAGTCATTGGGGGTACTATAAATGAAAATGGTGTGTTGCATATCAAGGCAACACGGGTTGAATCAGAAAGTTCCCTTGCGCAGATTGTACGGCTTGTTGAGTCAGCTCAGATGGCAAAAGCTCCTGTTCAGAAGTTTGCTGACCGCATTTCTAAATTCTTTGTGCCACTGGTGATCATGCTTTCATTCTTTACCTGGCTTTCATGGTTTTTGGCTGGAAAATTCCATGGCTACCCGAAATCTTGGATACCGAAGTCCATGGATAGCTTTCAGCTTGCTCTCCAGTTTGGGATCTCTGTCATGGTTATTGCGTGCCCTTGTGCTCTAGGCCTAGCAACTCCTACTGCTGTCATGGTTGGCACTGGAGTCGGTGCATCCCAAGGTGTACTGATCAAAGGAGGTCAAGCATTAGAAAGTGCACATAAGGTGGACTGCATTGTGTTCGACAAGACAGGGACTCTTACAATTGGGAAGCCACTGGTTGTTAACAAAAAAAAAATGTTAAAAAAAGGTGGACGATTGTGTTCGAAAGACAGGGACTTTACAATTGGGAAGCCACTGGTTGTTAACACACGACTCCTGAAAAATATGGTGTTTCGAGAATTCTATGAACTTGTTGCTGCAGCTGAGGTTAACAGTGAACACCCCTTGGCCAAGGCCATTGTTGAGTATGCCAAAAAATTCAGAGAAGATGAAGAGAATCCAGCCTGGCCAGAAGCGCATGACTTTGCCTCCATTACTGGTCACGGGGTGAAGGCTATTGTTCGGGGCAGGGAAATAATTGTGGGGAACAAGAGCTTGATGGTGGACCAGAACATTGCAGTTCCACTCGATGCAGAAGACTACCTAGCAGAAGCTGAAGGGCTAGCTCAAACTGGGATTCTAGTAGCCATAGATGGAGAAGTGGCCGGAGTTCTAGCAATATCTGATCCACTGAAACCAGGTGCTCAAGAAGTAATTACCATACTCAAGTCAATGAATGTTACAAGCATAATGGTGACAGGTGACAATTGGGGAACTGCAAATTCTATTGCCAATGAAGTTGGTATTGATACTGTTATAGCCGAAGCGAAACCTGACCAGAAAGCAGAGCAAGTGAAGGAATTGCAGGCTTCAGGCTACACTGTGGCGATGGTGGGAGATGGCATCAATGACTCACCAGCACTTGTGGCGGCAGATGTAGGAATGGCAATTGGTGCTGGCACTGACATTGCAATTGAGGCAGCAGACATTGTGCTGATGAAGAGCAACTTGGAGGATGTGATAACTGCCATTGATCTTTCACGGAAAACCTTCACTCGTATTCGTTTGAACTACATTTGGTCTTTGGGATATAATGTGCTCGGGATCCCAATAGCGGCAGGAGTTCTCTTCCCATCCACTGGATTTCGTTTACCCCTTGGATTGCTGGAGCTGCAATGGCTGCTTCTTCTGTCAGTGTTGTTTGCTGTTCATTGTTGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0008150 GO:0008324 GO:0009636 GO:0010035 GO:0010038 GO:0010273 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042221 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046688 GO:0046873 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0061687 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0097501 GO:0098655 GO:0098660 GO:0098662 GO:0098754 GO:0099131 GO:0099132 GO:1990169

KEGG Pathways

Metabolic & Signaling

ko01524 ko04016 map01524 map04016

Pfam Domains

Protein Families

E1-E2_ATPase HMA Hydrolase

Protein Analysis

975

Amino Acids

105.82

Weight (kDa)

6.56

Isoelectric Point (pI)

28.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMA	PF00403	33 - 93	2.1e-14	Heavy-metal-associated domain
HMA	PF00403	112 - 172	1.7e-12	Heavy-metal-associated domain
E1-E2_ATPase	PF00122	417 - 522	1e-21	P-type ATPase actuator domain
Hydrolase	PF00702	631 - 881	1.9e-33	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0002025)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G63440
fragaria_vesca	FvH4_5g02090 FvH4_5g02090 FvH4_5g02091 FvH4_5g02091 FvH4_5g02091 FvH4_5g02091
malus_domestica	MD06G1080800.v1.1 MD06G1080900.v1.1 MD14G1102600.v1.1
prunus_persica	Prupe.5G097900_v2.0.a1 Prupe.5G097900_v2.0.a1 Prupe.5G098000_v2.0.a1
pyrus_communis	pycom06g07780 pycom06g07790 pycom14g09890
rosa_chinensis	RchiOBHm_Chr7g0197781 RchiOBHm_Chr7g0198111 RchiOBHm_Chr7g0198141
rosa_laevigata	RLG00000003885 RLG00000003888 RLG00000019170
rosa_multiflora	Rmu_sc0000221.1_g000018 Rmu_sc0000221.1_g000030 Rmu_sc0006732.1_g000016
rosa_roxburghii	Rroxscaffold_3G00257940 Rroxscaffold_3G00257970 Rroxscaffold_3G00257990
rosa_rugosa	Rorug07G0045700 Rorug07G0045700 Rorug07G0045800 Rorug07G0045800 Rorug07G0045900
rosa_samantha	Rh7AG169100 Rh7AG169200 Rh7BG169900 Rh7BG172300 Rh7CG178600 Rh7CG178700 Rh7DG169200 Rh7DG171400 Rh7DG171800
rosa_wichuraiana	Rw7G014570 Rw7G014780 Rw7G014790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	950
Acc16I	TGCGCA	1 cut(s)	1545
Acc36I	ACCTGC	1 cut(s)	128
AccB1I	GGYRCC	2 cut(s)	80, 1249
AccB7I	CCANNNNNTGG	2 cut(s)	770, 2846
AccI	GTMKAC	4 cut(s)	189, 642, 965, 1092
AccII	CGCG	2 cut(s)	134, 1438
AciI	CCGC	8 cut(s)	56, 126, 134, 182, 768, 1609, 2636, 2822
AclWI	GGATC	9 cut(s)	145, 347, 452, 729, 742, 1763, 2387, 2806, 2819
AcoI	YGGCCR	3 cut(s)	2115, 2174, 2370
AcsI	RAATTY	8 cut(s)	666, 756, 804, 1619, 1685, 2066, 2144, 2488
AcuI	CTGAAG	3 cut(s)	456, 2343, 2562
AdeI	CACNNNGTG	1 cut(s)	2215
AfaI	GTAC	8 cut(s)	815, 884, 921, 1101, 1135, 1480, 1554, 1851
AfiI	CCNNNNNNNGG	3 cut(s)	770, 2821, 2846
AflII	CTTAAG	1 cut(s)	896
AflIII	ACRYGT	1 cut(s)	1293
AhlI	ACTAGT	1 cut(s)	1151
AjiI	CACGTC	1 cut(s)	193
AjnI	CCWGG	8 cut(s)	625, 737, 763, 889, 1357, 1658, 2171, 2404
AjuI	GAANNNNNNNTTGG	6 cut(s)	83, 115, 305, 337, 2699, 2731
AleI	CACNNNNGTG	1 cut(s)	323
Alw21I	GWGCWC	5 cut(s)	198, 1789, 1885, 2413, 2808
Alw26I	GTCTC	5 cut(s)	56, 239, 251, 615, 1142
Alw44I	GTGCAC	1 cut(s)	1881
AlwI	GGATC	9 cut(s)	145, 347, 452, 729, 742, 1763, 2387, 2806, 2819
AlwNI	CAGNNNCTG	1 cut(s)	2321
Ama87I	CYCGRG	2 cut(s)	167, 2807
AoxI	GGCC	9 cut(s)	11, 93, 698, 988, 1792, 2115, 2121, 2174, 2370
ApaI	GGGCCC	1 cut(s)	702
ApaLI	GTGCAC	1 cut(s)	1881
ApeKI	GCWGC	8 cut(s)	474, 1073, 1109, 2084, 2087, 2687, 2878, 2887
ApoI	RAATTY	8 cut(s)	666, 756, 804, 1619, 1685, 2066, 2144, 2488
ArsI	GACNNNNNNTTYG	2 cut(s)	494, 526
AseI	ATTAAT	1 cut(s)	528
Asp700I	GAANNNNTTC	2 cut(s)	667, 2756
AspLEI	GCGC	5 cut(s)	134, 1026, 1438, 1546, 2185
AspS9I	GGNCC	4 cut(s)	698, 699, 874, 2272
AsuC2I	CCSGG	2 cut(s)	168, 169
AsuHPI	GGTGA	9 cut(s)	548, 838, 1313, 1472, 1648, 2227, 2473, 2480, 2613
AsuII	TTCGAA	1 cut(s)	1991
AsuNHI	GCTAGC	1 cut(s)	2329
AvaI	CYCGRG	2 cut(s)	167, 2807
AvaII	GGWCC	2 cut(s)	874, 2272
BaeGI	GKGCMC	5 cut(s)	83, 211, 702, 1781, 1885
BaeI	ACNNNNGTAYC	2 cut(s)	1703, 1736
BalI	TGGCCA	2 cut(s)	2117, 2176
BamHI	GGATCC	2 cut(s)	734, 2811
BanI	GGYRCC	2 cut(s)	80, 1249
BanII	GRGCYC	2 cut(s)	418, 702
BbsI	GAAGAC	3 cut(s)	507, 546, 2310
Bbv12I	GWGCWC	5 cut(s)	198, 1789, 1885, 2413, 2808
BbvCI	CCTCAGC	1 cut(s)	2091
BbvI	GCAGC	8 cut(s)	461, 1085, 1096, 2071, 2099, 2699, 2865, 2874
BceAI	ACGGC	3 cut(s)	26, 80, 1571
BcgI	CGANNNNNNTGC	4 cut(s)	1813, 1847, 1888, 1922
BciT130I	CCWGG	8 cut(s)	627, 739, 765, 891, 1359, 1660, 2173, 2406
BciVI	GTATCC	1 cut(s)	1704
BclI	TGATCA	3 cut(s)	583, 1638, 1854
BcnI	CCSGG	2 cut(s)	168, 169
BcoDI	GTCTC	5 cut(s)	56, 239, 251, 615, 1142
BcuI	ACTAGT	1 cut(s)	1151
BfaI	CTAG	8 cut(s)	1152, 1193, 1790, 1796, 2312, 2330, 2348, 2381
BfmI	CTRYAG	2 cut(s)	817, 2085
BfrI	CTTAAG	1 cut(s)	896
BfuAI	ACCTGC	1 cut(s)	128
BfuI	GTATCC	1 cut(s)	1704
BglII	AGATCT	1 cut(s)	556
BlpI	GCTNAGC	1 cut(s)	932
BmcAI	AGTACT	2 cut(s)	815, 921
Bme18I	GGWCC	2 cut(s)	874, 2272
BmeT110I	CYCGRG	2 cut(s)	167, 2807
BmgBI	CACGTC	1 cut(s)	193
BmgT120I	GGNCC	4 cut(s)	698, 699, 874, 2272
BmiI	GGNNCC	6 cut(s)	82, 700, 701, 736, 1251, 2813
BmrI	ACTGGG	2 cut(s)	1014, 2349
BmsI	GCATC	4 cut(s)	1213, 1847, 2287, 2619
BmtI	GCTAGC	1 cut(s)	2333
BmuI	ACTGGG	2 cut(s)	1014, 2349
BpiI	GAAGAC	3 cut(s)	507, 546, 2310
BplI	GAGNNNNNCTC	2 cut(s)	813, 845
BpmI	CTGGAG	4 cut(s)	853, 1730, 1848, 2894
Bpu10I	CCTNAGC	3 cut(s)	427, 1206, 2091
Bpu1102I	GCTNAGC	1 cut(s)	932
Bpu14I	TTCGAA	1 cut(s)	1991
BpuEI	CTTGAG	2 cut(s)	2397, 2417
BpuMI	CCSGG	2 cut(s)	168, 169
BsaWI	WCCGGW	1 cut(s)	970
BsaXI	ACNNNNNCTCC	4 cut(s)	8, 38, 2180, 2210
Bsc4I	CCNNNNNNNGG	3 cut(s)	770, 2821, 2846
Bse3DI	GCAATG	5 cut(s)	1019, 1164, 2280, 2673, 2888
BseBI	CCWGG	8 cut(s)	627, 739, 765, 891, 1359, 1660, 2173, 2406
BseGI	GGATG	6 cut(s)	885, 1051, 1384, 1838, 2728, 2840
BseLI	CCNNNNNNNGG	3 cut(s)	770, 2821, 2846
BseMI	GCAATG	5 cut(s)	1019, 1164, 2280, 2673, 2888
BseMII	CTCAG	4 cut(s)	946, 1094, 1586, 2082
BseRI	GAGGAG	1 cut(s)	74
BseSI	GKGCMC	5 cut(s)	83, 211, 702, 1781, 1885
BseXI	GCAGC	8 cut(s)	461, 1085, 1096, 2071, 2099, 2699, 2865, 2874
BsgI	GTGCAG	2 cut(s)	349, 995
Bsh1236I	CGCG	2 cut(s)	134, 1438
BshFI	GGCC	9 cut(s)	13, 95, 700, 990, 1794, 2117, 2123, 2176, 2372
BshNI	GGYRCC	2 cut(s)	80, 1249
BsiHKAI	GWGCWC	5 cut(s)	198, 1789, 1885, 2413, 2808
BsiHKCI	CYCGRG	2 cut(s)	167, 2807
BsiSI	CCGG	4 cut(s)	168, 971, 991, 2373
BslFI	GGGAC	3 cut(s)	1410, 1933, 2015
BslI	CCNNNNNNNGG	3 cut(s)	770, 2821, 2846
BsmAI	GTCTC	5 cut(s)	56, 239, 251, 615, 1142
BsmFI	GGGAC	3 cut(s)	1410, 1933, 2015
BsnI	GGCC	9 cut(s)	13, 95, 700, 990, 1794, 2117, 2123, 2176, 2372
BsoBI	CYCGRG	2 cut(s)	167, 2807
Bsp119I	TTCGAA	1 cut(s)	1991
Bsp120I	GGGCCC	1 cut(s)	698
Bsp1407I	TGTACA	1 cut(s)	882
Bsp1720I	GCTNAGC	1 cut(s)	932
Bsp19I	CCATGG	4 cut(s)	870, 1027, 1690, 1722
BspACI	CCGC	8 cut(s)	56, 126, 134, 182, 768, 1609, 2636, 2822
BspANI	GGCC	9 cut(s)	13, 95, 700, 990, 1794, 2117, 2123, 2176, 2372
BspCNI	CTCAG	4 cut(s)	945, 1095, 1585, 2083
BspFNI	CGCG	2 cut(s)	134, 1438
BspHI	TCATGA	1 cut(s)	652
BspLI	GGNNCC	6 cut(s)	82, 700, 701, 736, 1251, 2813
BspMAI	CTGCAG	1 cut(s)	2089
BspMI	ACCTGC	1 cut(s)	128
BspOI	GCTAGC	1 cut(s)	2333
BspPI	GGATC	9 cut(s)	145, 347, 452, 729, 742, 1763, 2387, 2806, 2819
BspQI	GCTCTTC	2 cut(s)	373, 2702
BspT104I	TTCGAA	1 cut(s)	1991
BspT107I	GGYRCC	2 cut(s)	80, 1249
BspTI	CTTAAG	1 cut(s)	896
BsrDI	GCAATG	5 cut(s)	1019, 1164, 2280, 2673, 2888
BsrGI	TGTACA	1 cut(s)	882
Bst2UI	CCWGG	8 cut(s)	627, 739, 765, 891, 1359, 1660, 2173, 2406
Bst4CI	ACNGT	7 cut(s)	206, 376, 572, 609, 2102, 2521, 2590
Bst6I	CTCTTC	6 cut(s)	373, 618, 775, 2154, 2702, 2840
BstAFI	CTTAAG	1 cut(s)	896
BstAPI	GCANNNNNTGC	4 cut(s)	120, 363, 2660, 2887
BstAUI	TGTACA	1 cut(s)	882
BstBI	TTCGAA	1 cut(s)	1991
BstC8I	GCNNGC	8 cut(s)	130, 393, 1012, 1740, 1777, 2331, 2574, 2665
BstDEI	CTNAG	7 cut(s)	427, 907, 932, 1103, 1206, 1572, 2091
BstDSI	CCRYGG	5 cut(s)	8, 870, 1027, 1690, 1722
BstF5I	GGATG	6 cut(s)	885, 1051, 1384, 1838, 2728, 2840
BstFNI	CGCG	2 cut(s)	134, 1438
BstHHI	GCGC	5 cut(s)	134, 1026, 1438, 1546, 2185
BstMAI	GTCTC	5 cut(s)	56, 239, 251, 615, 1142
BstNI	CCWGG	8 cut(s)	627, 739, 765, 891, 1359, 1660, 2173, 2406
BstNSI	RCATGY	1 cut(s)	1014
BstSFI	CTRYAG	2 cut(s)	817, 2085
BstSLI	GKGCMC	5 cut(s)	83, 211, 702, 1781, 1885
BstUI	CGCG	2 cut(s)	134, 1438
BstV1I	GCAGC	8 cut(s)	461, 1085, 1096, 2071, 2099, 2699, 2865, 2874
BstV2I	GAAGAC	3 cut(s)	507, 546, 2310
BstX2I	RGATCY	4 cut(s)	556, 734, 1755, 2811
BstXI	CCANNNNNNTGG	1 cut(s)	1240
BstYI	RGATCY	4 cut(s)	556, 734, 1755, 2811
BsuI	GTATCC	1 cut(s)	1704
BsuRI	GGCC	9 cut(s)	13, 95, 700, 990, 1794, 2117, 2123, 2176, 2372
BtgI	CCRYGG	5 cut(s)	8, 870, 1027, 1690, 1722
BtgZI	GCGATG	1 cut(s)	2609
BtrI	CACGTC	1 cut(s)	193
BtsCI	GGATG	6 cut(s)	885, 1051, 1384, 1838, 2728, 2840
BveI	ACCTGC	1 cut(s)	128
Cac8I	GCNNGC	8 cut(s)	130, 393, 1012, 1740, 1777, 2331, 2574, 2665
CaiI	CAGNNNCTG	1 cut(s)	2321
CciI	TCATGA	1 cut(s)	652
CfoI	GCGC	5 cut(s)	134, 1026, 1438, 1546, 2185
Cfr13I	GGNCC	4 cut(s)	698, 699, 874, 2272
Cfr9I	CCCGGG	1 cut(s)	167
CseI	GACGC	1 cut(s)	1427
CsiI	ACCWGGT	1 cut(s)	2404
Csp6I	GTAC	8 cut(s)	814, 883, 920, 1100, 1134, 1479, 1553, 1850
CviQI	GTAC	8 cut(s)	814, 883, 920, 1100, 1134, 1479, 1553, 1850
DdeI	CTNAG	7 cut(s)	427, 907, 932, 1103, 1206, 1572, 2091
DraIII	CACNNNGTG	1 cut(s)	2215
EaeI	YGGCCR	3 cut(s)	2115, 2174, 2370
Eam1104I	CTCTTC	6 cut(s)	373, 618, 775, 2154, 2702, 2840
EarI	CTCTTC	6 cut(s)	373, 618, 775, 2154, 2702, 2840
EciI	GGCGGA	2 cut(s)	71, 115
Eco147I	AGGCCT	1 cut(s)	1794
Eco24I	GRGCYC	2 cut(s)	418, 702
Eco47I	GGWCC	2 cut(s)	874, 2272
Eco57I	CTGAAG	3 cut(s)	456, 2343, 2562
Eco88I	CYCGRG	2 cut(s)	167, 2807
EcoO109I	RGGNCCY	2 cut(s)	698, 699
EcoRI	GAATTC	1 cut(s)	2066
EcoRII	CCWGG	8 cut(s)	625, 737, 763, 889, 1357, 1658, 2171, 2404
EcoT38I	GRGCYC	2 cut(s)	418, 702
FalI	AAGNNNNNCTT	6 cut(s)	507, 539, 890, 922, 2699, 2731
FaqI	GGGAC	3 cut(s)	1410, 1933, 2015
FauI	CCCGC	1 cut(s)	127
FbaI	TGATCA	3 cut(s)	583, 1638, 1854
FblI	GTMKAC	4 cut(s)	189, 642, 965, 1092
FokI	GGATG	6 cut(s)	872, 1058, 1391, 1825, 2735, 2827
FriOI	GRGCYC	2 cut(s)	418, 702
FspBI	CTAG	8 cut(s)	1152, 1193, 1790, 1796, 2312, 2330, 2348, 2381
FspI	TGCGCA	1 cut(s)	1545
GlaI	GCGC	5 cut(s)	133, 1025, 1437, 1545, 2184
GsuI	CTGGAG	4 cut(s)	853, 1730, 1848, 2894
HaeIII	GGCC	9 cut(s)	13, 95, 700, 990, 1794, 2117, 2123, 2176, 2372
HapII	CCGG	4 cut(s)	168, 971, 991, 2373
HgaI	GACGC	1 cut(s)	1427
HhaI	GCGC	5 cut(s)	134, 1026, 1438, 1546, 2185
Hin6I	GCGC	5 cut(s)	132, 1024, 1436, 1544, 2183
HinP1I	GCGC	5 cut(s)	132, 1024, 1436, 1544, 2183
HincII	GTYRAC	7 cut(s)	190, 238, 568, 1272, 1951, 2032, 2098
HindII	GTYRAC	7 cut(s)	190, 238, 568, 1272, 1951, 2032, 2098
HpaI	GTTAAC	4 cut(s)	238, 1951, 2032, 2098
HpaII	CCGG	4 cut(s)	168, 971, 991, 2373
HphI	GGTGA	9 cut(s)	548, 838, 1313, 1472, 1648, 2227, 2473, 2480, 2613
Hpy188I	TCNGA	8 cut(s)	42, 1222, 1378, 1531, 1575, 1596, 2150, 2392
Hpy188III	TCNNGA	7 cut(s)	176, 653, 832, 2044, 2063, 2414, 2809
Hpy99I	CGWCG	1 cut(s)	194
HpyCH4III	ACNGT	7 cut(s)	206, 376, 572, 609, 2102, 2521, 2590
HpyCH4IV	ACGT	3 cut(s)	192, 320, 1293
HpyF3I	CTNAG	7 cut(s)	427, 907, 932, 1103, 1206, 1572, 2091
HpySE526I	ACGT	3 cut(s)	192, 320, 1293
HspAI	GCGC	5 cut(s)	132, 1024, 1436, 1544, 2183
Ksp22I	TGATCA	3 cut(s)	583, 1638, 1854
KspAI	GTTAAC	4 cut(s)	238, 1951, 2032, 2098
LguI	GCTCTTC	2 cut(s)	373, 2702
LmnI	GCTCC	5 cut(s)	128, 773, 943, 1591, 2875
Lsp1109I	GCAGC	8 cut(s)	461, 1085, 1096, 2071, 2099, 2699, 2865, 2874
LweI	GCATC	4 cut(s)	1213, 1847, 2287, 2619
MabI	ACCWGGT	1 cut(s)	2404
MaeI	CTAG	8 cut(s)	1152, 1193, 1790, 1796, 2312, 2330, 2348, 2381
MaeII	ACGT	3 cut(s)	192, 320, 1293
MaeIII	GTNAC	4 cut(s)	2207, 2446, 2461, 2468
MfeI	CAATTG	5 cut(s)	1929, 2010, 2473, 2655, 2679
MflI	RGATCY	4 cut(s)	556, 734, 1755, 2811
MlsI	TGGCCA	2 cut(s)	2117, 2176
MluNI	TGGCCA	2 cut(s)	2117, 2176
MlyI	GAGTC	8 cut(s)	95, 831, 1267, 1574, 1839, 1915, 2034, 2612
MmeI	TCCRAC	1 cut(s)	253
Mox20I	TGGCCA	2 cut(s)	2117, 2176
MroXI	GAANNNNTTC	2 cut(s)	667, 2756
MscI	TGGCCA	2 cut(s)	2117, 2176
MslI	CAYNNNNRTG	6 cut(s)	113, 323, 654, 716, 1410, 2459
Msp20I	TGGCCA	2 cut(s)	2117, 2176
MspA1I	CMGCKG	2 cut(s)	484, 2090
MspCI	CTTAAG	1 cut(s)	896
MspI	CCGG	4 cut(s)	168, 971, 991, 2373
MunI	CAATTG	5 cut(s)	1929, 2010, 2473, 2655, 2679
MvaI	CCWGG	8 cut(s)	627, 739, 765, 891, 1359, 1660, 2173, 2406
MvnI	CGCG	2 cut(s)	134, 1438
NciI	CCSGG	2 cut(s)	168, 169
NcoI	CCATGG	4 cut(s)	870, 1027, 1690, 1722
NheI	GCTAGC	1 cut(s)	2329
NlaIV	GGNNCC	6 cut(s)	82, 700, 701, 736, 1251, 2813
NmuCI	GTSAC	3 cut(s)	2207, 2461, 2468
NsbI	TGCGCA	1 cut(s)	1545
NspI	RCATGY	1 cut(s)	1014
NspV	TTCGAA	1 cut(s)	1991
OliI	CACNNNNGTG	1 cut(s)	323
PaeI	GCATGC	1 cut(s)	1014
PagI	TCATGA	1 cut(s)	652
PasI	CCCWGGG	1 cut(s)	626
PceI	AGGCCT	1 cut(s)	1794
PciSI	GCTCTTC	2 cut(s)	373, 2702
PdmI	GAANNNNTTC	2 cut(s)	667, 2756
PfeI	GAWTC	6 cut(s)	172, 380, 958, 1526, 2164, 2344
PflMI	CCANNNNNTGG	2 cut(s)	770, 2846
PleI	GAGTC	8 cut(s)	94, 830, 1267, 1573, 1838, 1915, 2034, 2612
PpsI	GAGTC	8 cut(s)	94, 830, 1267, 1573, 1838, 1915, 2034, 2612
PshBI	ATTAAT	1 cut(s)	528
PsiI	TTATAA	1 cut(s)	950
Psp6I	CCWGG	8 cut(s)	625, 737, 763, 889, 1357, 1658, 2171, 2404
PspGI	CCWGG	8 cut(s)	625, 737, 763, 889, 1357, 1658, 2171, 2404
PspN4I	GGNNCC	6 cut(s)	82, 700, 701, 736, 1251, 2813
PspOMI	GGGCCC	1 cut(s)	698
PspPI	GGNCC	4 cut(s)	698, 699, 874, 2272
PstI	CTGCAG	1 cut(s)	2089
PstNI	CAGNNNCTG	1 cut(s)	2321
PsuI	RGATCY	4 cut(s)	556, 734, 1755, 2811
PvuII	CAGCTG	2 cut(s)	484, 2090
RsaI	GTAC	8 cut(s)	815, 884, 921, 1101, 1135, 1480, 1554, 1851
RsaNI	GTAC	8 cut(s)	814, 883, 920, 1100, 1134, 1479, 1553, 1850
RseI	CAYNNNNRTG	6 cut(s)	113, 323, 654, 716, 1410, 2459
SalI	GTCGAC	1 cut(s)	188
SapI	GCTCTTC	2 cut(s)	373, 2702
Sau96I	GGNCC	4 cut(s)	698, 699, 874, 2272
ScaI	AGTACT	2 cut(s)	815, 921
SchI	GAGTC	8 cut(s)	95, 831, 1267, 1574, 1839, 1915, 2034, 2612
SexAI	ACCWGGT	1 cut(s)	2404
SfaNI	GCATC	4 cut(s)	1213, 1847, 2287, 2619
SfcI	CTRYAG	2 cut(s)	817, 2085
SfuI	TTCGAA	1 cut(s)	1991
SinI	GGWCC	2 cut(s)	874, 2272
SmaI	CCCGGG	1 cut(s)	169
SmiMI	CAYNNNNRTG	6 cut(s)	113, 323, 654, 716, 1410, 2459
SmlI	CTYRAG	3 cut(s)	896, 2412, 2432
SmoI	CTYRAG	3 cut(s)	896, 2412, 2432
SpeI	ACTAGT	1 cut(s)	1151
SphI	GCATGC	1 cut(s)	1014
SseBI	AGGCCT	1 cut(s)	1794
SsiI	CCGC	8 cut(s)	56, 126, 134, 182, 768, 1609, 2636, 2822
SspMI	CTAG	8 cut(s)	1152, 1193, 1790, 1796, 2312, 2330, 2348, 2381
StuI	AGGCCT	1 cut(s)	1794
TaaI	ACNGT	7 cut(s)	206, 376, 572, 609, 2102, 2521, 2590
TaiI	ACGT	3 cut(s)	195, 323, 1296
TaqI	TCGA	5 cut(s)	189, 1908, 1991, 2062, 2295
TatI	WGTACW	4 cut(s)	813, 882, 919, 1849
TauI	GCSGC	2 cut(s)	2639, 2825
TfiI	GAWTC	6 cut(s)	172, 380, 958, 1526, 2164, 2344
TseFI	GTSAC	3 cut(s)	2207, 2461, 2468
TseI	GCWGC	8 cut(s)	474, 1073, 1109, 2084, 2087, 2687, 2878, 2887
Tsp45I	GTSAC	3 cut(s)	2207, 2461, 2468
TspGWI	ACGGA	4 cut(s)	94, 242, 1087, 2764
TspMI	CCCGGG	1 cut(s)	167
Van91I	CCANNNNNTGG	2 cut(s)	770, 2846
Vha464I	CTTAAG	1 cut(s)	896
VneI	GTGCAC	1 cut(s)	1881
VpaK11BI	GGWCC	2 cut(s)	874, 2272
VspI	ATTAAT	1 cut(s)	528
XapI	RAATTY	8 cut(s)	666, 756, 804, 1619, 1685, 2066, 2144, 2488
XceI	RCATGY	1 cut(s)	1014
XmaI	CCCGGG	1 cut(s)	167
XmiI	GTMKAC	4 cut(s)	189, 642, 965, 1092
XmnI	GAANNNNTTC	2 cut(s)	667, 2756
XspI	CTAG	8 cut(s)	1152, 1193, 1790, 1796, 2312, 2330, 2348, 2381
ZrmI	AGTACT	2 cut(s)	815, 921

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.