Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0002025

Physical Location & Seq

Genomic Coordinates Forward (+)

52352088 .. 52373078

Gene Structure 20991 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00257990.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2955 bp

ATGAGTTGCATTTGCAGGGATTGCTTGGGTGGCATGTTACCCGGGCCACGGCCACACTACCCCTCGATGCCCCGATACCCGAAAGGCACGTCTCCAGAAGCAACGAGGAAGGCCAGGGGTTCTGAGGTGAAGGCCTTGTTCTCTGTGGTTGGAATGACATGTGCTGCCTGTGCCGGGTCTGTGGAGAAGGCGGTGAAGCGGCTCCCGGGGATACAGGAGGCCGCCGTGGACGTGTTGAACAACACAGCCCTTGTTCTCTACTATCCCAGCTTCGTCAATGAGGACAAAATATGTGAGGCTGTTAAAAATGCTGGATTTGAAGCTAAACTGATTAAGCTGGAGATGAATGACAAAACCAGACAAGTATGCAGAATAAGCATAAGTGGAATGACTTGCACGTCTTGCTCCTCCACTGTTGAATCAGCTCTGCAAGCAATTCATGGAGTGCAAGGAGCTCAGGTGGCCTTAGCAACTGAAGAAGCAGAGATTCATTATGACCCAAGCATTGTTACCTCCCATCAGCTATTGGAAACCATAGAAATCACCGGATTCGAAGCCAAACTTATTAGTTTAGGGGAAGGCATTAGCAAGATAGAGCTTCAAGTTGATGGCATAAAGGTTGGACAGTCGATTAGAGCAATAGCAAAAACTCTTGAAGCACTTCCTGGGGTTCAAGATATAGAGACATTTCCTGAACTCAACAAGATTTCTATTTCTTATAAACCAGAGATGATAGGACCAAGAACCCTCATCGAAGCCATCGAGTCATCAGGGTCCGCGCATTTCAAAGCAATGATATACCCGGAAGGAGAAGAAGACACTCATAGACAGAAGGAAATTAAGCAATACTATAAATTCTTCTTATGGAGTTTGTTTTGTACAATTCCTGTGTTTTTAACCTCCATGGTCTTGATGTATATTCCTGGAATTAAGAAGGTGTTGGATGTCAAAATTGTGAATAAGTTGAGTGTTGGTCAGATTCTGAGGTGGGAGTTGTCCACTCCTGTGCAGTTCATCATAGGCAAGAGGTTCTACATTGGAGCATACAAAGCATTGCGCCATGGTTCAGCTAACATGGATGTTTTGATTGCCTTGGGAACAAATGCAGCCTACTTCTACTCGGTCTACATAGTTTTAAGATCTGCAACCCATAAGGATTTCAAAGGTACAGATTTTTTTGAGACTAGTTCAATGCTTATTACATTCATCCTGTTAGGAAAGTATCTAGAGGTCTTGGCAAAAGGCAAGACATCTGAGGCCATTGCCAAGCTAATGGACTTGGCACCAGAAAAGGCAACACTGCTAACTTTGGGTGAGGAAGGAAATGTGATAAATGAACAAGAAATGGATAGCCGGTTGATACAAAAGAACGATCTGATTAAAATTATTCCTGGTGCCAAAGTAGCTTGTGATGGTGTTGTTATGTGGGGACAAAGTCATGTGAATGAAAGCATGATAACTGGAGAAGCACGGCCAGTGACAAAAAAGAAGGATGATGCAGTTATTGGAGGCACCGTGAATGAGAATGGAGTGTTGCATATTAAGGCAACAAGAGTTGGGTCAGAGAGTGCTCTTTCACAGATTGTAAGGCTTGTCGAGTCTGCCCAGATGGCTAAAGCTCCTGTACAAAAGTTTGCTGATCGCATTTCCAAATACTTTGTGCCAGTGGTCATCACACTATCATTTTCGACTTGGCTGGCCTGGTTCTTAGGAGGGAAGTTCCACAGGTATCCACGTTCTTGGATACCATCTTCAATCGATAGTTTTGAGCTTGCTCTTCAGTTTGGGATCTCGGTCATGGTCATTGCCTGCCCTTGTGCTTTAGGCCTAGCAACTCCTACAGCCGTTATGGTTGGTACAGGAGTTGGTGCATCGCAAGGTATATTGATCAAAGGCGGACATGCATTACAGAGTGCGCACAAGGTGAACTGCATTGTGTTTGACAAGACAGGGACTCTCACAGTTGGGAAGCCAGTGGTTGTTAGCACCAAAATCTTTAACCAAATGCTACCTCAAGAATTCTATGAACTAGTTGCTGCAACTGAGGTAAATAGTGAGCACCCACTAGCCAAAGCTATTGTGCAATATGCCAAGAAAATCAGAGAAGATGAAGAGAATTCTACCTGGCCAGAAGCACAAAACTTCGCATCCCTTACCGGCCAAGGAGTGAAGGCAGTTGTTCGAAATAAGGAAATAACTGTAGGCAACAAGAGCTTAATGTTGGACATTAACATTGCGATTCCAGAGGAGGCTGAAGTTGCACTTGCACAAACTGAAGCATTGGCTCAAACTGGGATTTTAATAGCTGTAGATAACGAAATAGCAGGAATTATAGCCATATCTGATCCACTGAAACCTGGTGCTCGAGAACTGATTTCCATACTCAAGTCTATGGGAGTTAGAAGTATCATGGTTACAGGTGACAATTGGGGTACAGCCAATTCTATTGCCGAGGAAACTGAAATTGAGACGGTGATTGCAGAAGCCAAACCCCAGCACAAAGCTGAGAAAATAAAAGATTTGCAGGCAAGATTTACTGCTTCAGGCTACACCGTGGCAATGGTGGGAGATGGGATCAATGACTCACCGGCACTTGTGGCAGCAGATGTTGGAATGGCAATTGGTGCTGGCACAGACATTGCGATCGAGGCAGCTGACATTGTTCTTATGAAGAGCAACTTGGAAGATGTTATAACAGCCATTGACCTTTCCCGGAAAACCTTTTCCCGTATTCGTCTGAACTACATTTGGGCATTGGGTTACAATGTGCTTGGAATCCCGATAGCTGCAGGGGTCCTTTTTCCCTCCACTAGGTTCAGATTACCACCATGGATTGCAGGAGCTGCAATGGCGGCATCCTCCGTTAGTGTTGTATGCTGTTCTCTGTTGTTGAAGAACTACAAGAGACCCAAGAAGTTGGATATCCTTGAGATACATGAAGTAAGGGTTGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0008150 GO:0008324 GO:0009636 GO:0010035 GO:0010038 GO:0010273 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042221 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046688 GO:0046873 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0061687 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0097501 GO:0098655 GO:0098660 GO:0098662 GO:0098754 GO:0099131 GO:0099132 GO:1990169

KEGG Pathways

Metabolic & Signaling

ko01524 ko04016 map01524 map04016

Pfam Domains

Protein Families

E1-E2_ATPase HMA Hydrolase

Protein Analysis

984

Amino Acids

106.72

Weight (kDa)

6.79

Isoelectric Point (pI)

37.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMA	PF00403	47 - 107	2.9e-14	Heavy-metal-associated domain
HMA	PF00403	125 - 185	1.3e-12	Heavy-metal-associated domain
E1-E2_ATPase	PF00122	427 - 533	5e-25	P-type ATPase actuator domain
Hydrolase	PF00702	643 - 869	2e-32	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0002025)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G63440
fragaria_vesca	FvH4_5g02090 FvH4_5g02090 FvH4_5g02091 FvH4_5g02091 FvH4_5g02091 FvH4_5g02091
malus_domestica	MD06G1080800.v1.1 MD06G1080900.v1.1 MD14G1102600.v1.1
prunus_persica	Prupe.5G097900_v2.0.a1 Prupe.5G097900_v2.0.a1 Prupe.5G098000_v2.0.a1
pyrus_communis	pycom06g07780 pycom06g07790 pycom14g09890
rosa_chinensis	RchiOBHm_Chr7g0197781 RchiOBHm_Chr7g0198111 RchiOBHm_Chr7g0198141
rosa_laevigata	RLG00000003885 RLG00000003888 RLG00000019170
rosa_multiflora	Rmu_sc0000221.1_g000018 Rmu_sc0000221.1_g000030 Rmu_sc0006732.1_g000016
rosa_roxburghii	Rroxscaffold_3G00257940 Rroxscaffold_3G00257970 Rroxscaffold_3G00257990
rosa_rugosa	Rorug07G0045700 Rorug07G0045700 Rorug07G0045800 Rorug07G0045800 Rorug07G0045900
rosa_samantha	Rh7AG169100 Rh7AG169200 Rh7BG169900 Rh7BG172300 Rh7CG178600 Rh7CG178700 Rh7DG169200 Rh7DG171400 Rh7DG171800
rosa_wichuraiana	Rw7G014570 Rw7G014780 Rw7G014790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	720, 2693
AasI	GACNNNNNNGTC	1 cut(s)	397
Acc16I	TGCGCA	1 cut(s)	1917
AccB1I	GGYRCC	3 cut(s)	1282, 1394, 1511
AccI	GTMKAC	1 cut(s)	1125
AccII	CGCG	1 cut(s)	779
AciI	CCGC	6 cut(s)	191, 199, 222, 777, 1896, 2852
AclWI	GGATC	3 cut(s)	1796, 2339, 2582
AcoI	YGGCCR	4 cut(s)	50, 1472, 2126, 2158
AcsI	RAATTY	3 cut(s)	854, 2018, 2116
AcuI	CTGAAG	5 cut(s)	495, 1763, 2274, 2295, 2526
AdeI	CACNNNGTG	1 cut(s)	1924
AfaI	GTAC	5 cut(s)	880, 1168, 1626, 1858, 2434
AfiI	CCNNNNNNNGG	3 cut(s)	48, 174, 2811
AflIII	ACRYGT	2 cut(s)	158, 231
AhlI	ACTAGT	2 cut(s)	1184, 2029
AjiI	CACGTC	3 cut(s)	90, 232, 399
AjnI	CCWGG	7 cut(s)	113, 664, 922, 1390, 1700, 2123, 2356
AjuI	GAANNNNNNNTTGG	2 cut(s)	2663, 2695
AleI	CACNNNNGTG	1 cut(s)	1004
Alw21I	GWGCWC	4 cut(s)	457, 1573, 2061, 2365
Alw26I	GTCTC	5 cut(s)	96, 677, 1175, 2462, 2899
AlwI	GGATC	3 cut(s)	1796, 2339, 2582
AlwNI	CAGNNNCTG	2 cut(s)	982, 2843
Ama87I	CYCGRG	3 cut(s)	41, 205, 2364
ApeKI	GCWGC	7 cut(s)	164, 1106, 2036, 2600, 2651, 2786, 2843
ApoI	RAATTY	3 cut(s)	854, 2018, 2116
ArsI	GACNNNNNNTTYG	2 cut(s)	74, 106
Asp700I	GAANNNNTTC	1 cut(s)	660
AspLEI	GCGC	3 cut(s)	781, 1059, 1918
AspS9I	GGNCC	4 cut(s)	44, 737, 774, 2794
AsuC2I	CCSGG	7 cut(s)	42, 43, 175, 206, 207, 803, 2713
AsuHPI	GGTGA	8 cut(s)	139, 205, 535, 1325, 1936, 2432, 2485, 2577
AsuII	TTCGAA	2 cut(s)	552, 2182
AvaI	CYCGRG	3 cut(s)	41, 205, 2364
AvaII	GGWCC	3 cut(s)	737, 774, 2794
BaeI	ACNNNNGTAYC	2 cut(s)	1848, 1881
BalI	TGGCCA	1 cut(s)	2128
BanI	GGYRCC	3 cut(s)	1282, 1394, 1511
BanII	GRGCYC	1 cut(s)	457
BbsI	GAAGAC	1 cut(s)	822
Bbv12I	GWGCWC	4 cut(s)	457, 1573, 2061, 2365
BbvI	GCAGC	7 cut(s)	151, 1118, 2023, 2612, 2663, 2773, 2830
BccI	CCATC	7 cut(s)	525, 602, 767, 1406, 1603, 1756, 2564
BceAI	ACGGC	4 cut(s)	65, 209, 1487, 1829
BciT130I	CCWGG	7 cut(s)	115, 666, 924, 1392, 1702, 2125, 2358
BciVI	GTATCC	3 cut(s)	204, 1737, 1740
BclI	TGATCA	1 cut(s)	1887
BcnI	CCSGG	7 cut(s)	42, 43, 175, 206, 207, 803, 2713
BcoDI	GTCTC	5 cut(s)	96, 677, 1175, 2462, 2899
BcuI	ACTAGT	2 cut(s)	1184, 2029
BfaI	CTAG	6 cut(s)	1185, 1226, 1829, 2030, 2066, 2811
BfmI	CTRYAG	4 cut(s)	1839, 2199, 2307, 2787
BfuI	GTATCC	3 cut(s)	204, 1737, 1740
BglI	GCCNNNNNGGC	1 cut(s)	1610
BglII	AGATCT	1 cut(s)	1139
Bme18I	GGWCC	3 cut(s)	737, 774, 2794
BmeT110I	CYCGRG	3 cut(s)	41, 205, 2364
BmgBI	CACGTC	3 cut(s)	90, 232, 399
BmgT120I	GGNCC	4 cut(s)	44, 737, 774, 2794
BmiI	GGNNCC	6 cut(s)	203, 775, 1284, 1396, 1513, 2795
BmrI	ACTGGG	1 cut(s)	2301
BmsI	GCATC	5 cut(s)	57, 1486, 1880, 2156, 2864
BmuI	ACTGGG	1 cut(s)	2301
BpiI	GAAGAC	1 cut(s)	822
BpmI	CTGGAG	3 cut(s)	78, 359, 1482
Bpu10I	CCTNAGC	2 cut(s)	456, 466
Bpu14I	TTCGAA	2 cut(s)	552, 2182
BpuEI	CTTGAG	3 cut(s)	1998, 2369, 2948
BpuMI	CCSGG	7 cut(s)	42, 43, 175, 206, 207, 803, 2713
Bsa29I	ATCGAT	1 cut(s)	1758
BsaI	GGTCTC	1 cut(s)	2899
BsaWI	WCCGGW	1 cut(s)	545
BsaXI	ACNNNNNCTCC	4 cut(s)	444, 474, 1500, 1530
Bsc4I	CCNNNNNNNGG	3 cut(s)	48, 174, 2811
Bse118I	RCCGGY	3 cut(s)	1353, 2156, 2587
Bse1I	ACTGG	5 cut(s)	1465, 1475, 1664, 1973, 2296
Bse3DI	GCAATG	8 cut(s)	798, 1052, 1260, 1803, 2232, 2565, 2637, 2853
BseBI	CCWGG	7 cut(s)	115, 666, 924, 1392, 1702, 2125, 2358
BseCI	ATCGAT	1 cut(s)	1758
BseGI	GGATG	6 cut(s)	949, 1084, 1206, 1498, 2147, 2855
BseLI	CCNNNNNNNGG	3 cut(s)	48, 174, 2811
BseMI	GCAATG	8 cut(s)	798, 1052, 1260, 1803, 2232, 2565, 2637, 2853
BseMII	CTCAG	6 cut(s)	114, 470, 974, 1245, 2034, 2496
BseNI	ACTGG	5 cut(s)	1465, 1475, 1664, 1973, 2296
BseRI	GAGGAG	2 cut(s)	397, 2261
BseXI	GCAGC	7 cut(s)	151, 1118, 2023, 2612, 2663, 2773, 2830
BseYI	CCCAGC	2 cut(s)	266, 2493
BsgI	GTGCAG	1 cut(s)	1028
Bsh1236I	CGCG	1 cut(s)	779
Bsh1285I	CGRYCG	1 cut(s)	2646
BshNI	GGYRCC	3 cut(s)	1282, 1394, 1511
BshVI	ATCGAT	1 cut(s)	1758
BsiEI	CGRYCG	1 cut(s)	2646
BsiHKAI	GWGCWC	4 cut(s)	457, 1573, 2061, 2365
BsiHKCI	CYCGRG	3 cut(s)	41, 205, 2364
BsiSI	CCGG	9 cut(s)	42, 174, 206, 546, 803, 1354, 2157, 2588, 2713
BslFI	GGGAC	2 cut(s)	1443, 1966
BslI	CCNNNNNNNGG	3 cut(s)	48, 174, 2811
BsmAI	GTCTC	5 cut(s)	96, 677, 1175, 2462, 2899
BsmBI	CGTCTC	2 cut(s)	96, 2462
BsmFI	GGGAC	2 cut(s)	1443, 1966
Bso31I	GGTCTC	1 cut(s)	2899
BsoBI	CYCGRG	3 cut(s)	41, 205, 2364
Bsp119I	TTCGAA	2 cut(s)	552, 2182
Bsp1286I	GDGCHC	4 cut(s)	457, 1573, 2061, 2365
Bsp1407I	TGTACA	2 cut(s)	878, 1624
Bsp143I	GATC	8 cut(s)	1139, 1372, 1639, 1788, 1887, 2344, 2574, 2643
Bsp19I	CCATGG	3 cut(s)	903, 1060, 2828
BspACI	CCGC	6 cut(s)	191, 199, 222, 777, 1896, 2852
BspCNI	CTCAG	6 cut(s)	115, 469, 975, 1246, 2035, 2497
BspDI	ATCGAT	1 cut(s)	1758
BspFNI	CGCG	1 cut(s)	779
BspLI	GGNNCC	6 cut(s)	203, 775, 1284, 1396, 1513, 2795
BspMAI	CTGCAG	1 cut(s)	2791
BspPI	GGATC	3 cut(s)	1796, 2339, 2582
BspQI	GCTCTTC	2 cut(s)	1782, 2666
BspT104I	TTCGAA	2 cut(s)	552, 2182
BspT107I	GGYRCC	3 cut(s)	1282, 1394, 1511
BspTNI	GGTCTC	1 cut(s)	2899
BsrDI	GCAATG	8 cut(s)	798, 1052, 1260, 1803, 2232, 2565, 2637, 2853
BsrFI	RCCGGY	3 cut(s)	1353, 2156, 2587
BsrGI	TGTACA	2 cut(s)	878, 1624
BsrI	ACTGG	5 cut(s)	1465, 1475, 1664, 1973, 2296
BssAI	RCCGGY	3 cut(s)	1353, 2156, 2587
BssMI	GATC	8 cut(s)	1139, 1372, 1639, 1788, 1887, 2344, 2574, 2643
BssT1I	CCWWGG	5 cut(s)	903, 1060, 1092, 2161, 2828
Bst2UI	CCWGG	7 cut(s)	115, 666, 924, 1392, 1702, 2125, 2358
Bst4CI	ACNGT	7 cut(s)	415, 627, 1516, 1963, 2200, 2473, 2554
Bst6I	CTCTTC	3 cut(s)	1782, 2106, 2666
BstAPI	GCANNNNNTGC	4 cut(s)	21, 402, 2624, 2843
BstAUI	TGTACA	2 cut(s)	878, 1624
BstBI	TTCGAA	2 cut(s)	552, 2182
BstC8I	GCNNGC	6 cut(s)	432, 1698, 1773, 1810, 2526, 2629
BstDEI	CTNAG	8 cut(s)	123, 456, 466, 983, 1254, 1708, 2043, 2505
BstDSI	CCRYGG	6 cut(s)	47, 225, 903, 1060, 2553, 2828
BstENI	CCTNNNNNAGG	1 cut(s)	2809
BstF5I	GGATG	6 cut(s)	949, 1084, 1206, 1498, 2147, 2855
BstFNI	CGCG	1 cut(s)	779
BstHHI	GCGC	3 cut(s)	781, 1059, 1918
BstKTI	GATC	8 cut(s)	1142, 1375, 1642, 1791, 1890, 2347, 2577, 2646
BstMAI	GTCTC	5 cut(s)	96, 677, 1175, 2462, 2899
BstMBI	GATC	8 cut(s)	1139, 1372, 1639, 1788, 1887, 2344, 2574, 2643
BstMCI	CGRYCG	1 cut(s)	2646
BstNI	CCWGG	7 cut(s)	115, 666, 924, 1392, 1702, 2125, 2358
BstNSI	RCATGY	3 cut(s)	37, 162, 1904
BstSFI	CTRYAG	4 cut(s)	1839, 2199, 2307, 2787
BstUI	CGCG	1 cut(s)	779
BstV1I	GCAGC	7 cut(s)	151, 1118, 2023, 2612, 2663, 2773, 2830
BstV2I	GAAGAC	1 cut(s)	822
BstX2I	RGATCY	2 cut(s)	1139, 1788
BstXI	CCANNNNNNTGG	3 cut(s)	1273, 1740, 2917
BstYI	RGATCY	2 cut(s)	1139, 1788
Bsu15I	ATCGAT	1 cut(s)	1758
BsuI	GTATCC	3 cut(s)	204, 1737, 1740
BsuTUI	ATCGAT	1 cut(s)	1758
BtgI	CCRYGG	6 cut(s)	47, 225, 903, 1060, 2553, 2828
BtgZI	GCGATG	1 cut(s)	1857
BtrI	CACGTC	3 cut(s)	90, 232, 399
BtsCI	GGATG	6 cut(s)	949, 1084, 1206, 1498, 2147, 2855
BtsI	GCAGTG	1 cut(s)	1298
BtsIMutI	CAGTG	6 cut(s)	411, 1298, 1482, 1671, 1980, 2348
Cac8I	GCNNGC	6 cut(s)	432, 1698, 1773, 1810, 2526, 2629
CaiI	CAGNNNCTG	2 cut(s)	982, 2843
CfoI	GCGC	3 cut(s)	781, 1059, 1918
Cfr10I	RCCGGY	3 cut(s)	1353, 2156, 2587
Cfr13I	GGNCC	4 cut(s)	44, 737, 774, 2794
Cfr9I	CCCGGG	2 cut(s)	41, 205
ClaI	ATCGAT	1 cut(s)	1758
CsiI	ACCWGGT	1 cut(s)	2356
Csp6I	GTAC	5 cut(s)	879, 1167, 1625, 1857, 2433
CviQI	GTAC	5 cut(s)	879, 1167, 1625, 1857, 2433
DdeI	CTNAG	8 cut(s)	123, 456, 466, 983, 1254, 1708, 2043, 2505
DpnI	GATC	8 cut(s)	1141, 1374, 1641, 1790, 1889, 2346, 2576, 2645
DpnII	GATC	8 cut(s)	1139, 1372, 1639, 1788, 1887, 2344, 2574, 2643
DraIII	CACNNNGTG	1 cut(s)	1924
DrdI	GACNNNNNNGTC	1 cut(s)	397
DseDI	GACNNNNNNGTC	1 cut(s)	397
EaeI	YGGCCR	4 cut(s)	50, 1472, 2126, 2158
Eam1104I	CTCTTC	3 cut(s)	1782, 2106, 2666
EarI	CTCTTC	3 cut(s)	1782, 2106, 2666
EciI	GGCGGA	1 cut(s)	1911
Ecl136II	GAGCTC	1 cut(s)	455
Eco130I	CCWWGG	5 cut(s)	903, 1060, 1092, 2161, 2828
Eco147I	AGGCCT	2 cut(s)	134, 1827
Eco24I	GRGCYC	1 cut(s)	457
Eco31I	GGTCTC	1 cut(s)	2899
Eco32I	GATATC	1 cut(s)	2923
Eco47I	GGWCC	3 cut(s)	737, 774, 2794
Eco53kI	GAGCTC	1 cut(s)	455
Eco57I	CTGAAG	5 cut(s)	495, 1763, 2274, 2295, 2526
Eco88I	CYCGRG	3 cut(s)	41, 205, 2364
EcoICRI	GAGCTC	1 cut(s)	455
EcoNI	CCTNNNNNAGG	1 cut(s)	2809
EcoO109I	RGGNCCY	1 cut(s)	2794
EcoRI	GAATTC	2 cut(s)	2018, 2116
EcoRII	CCWGG	7 cut(s)	113, 664, 922, 1390, 1700, 2123, 2356
EcoRV	GATATC	1 cut(s)	2923
EcoT14I	CCWWGG	5 cut(s)	903, 1060, 1092, 2161, 2828
EcoT22I	ATGCAT	1 cut(s)	1906
EcoT38I	GRGCYC	1 cut(s)	457
ErhI	CCWWGG	5 cut(s)	903, 1060, 1092, 2161, 2828
Esp3I	CGTCTC	2 cut(s)	96, 2462
FalI	AAGNNNNNCTT	6 cut(s)	546, 578, 2247, 2279, 2663, 2695
FaqI	GGGAC	2 cut(s)	1443, 1966
FbaI	TGATCA	1 cut(s)	1887
FblI	GTMKAC	1 cut(s)	1125
FokI	GGATG	6 cut(s)	956, 1091, 1193, 1505, 2134, 2842
FriOI	GRGCYC	1 cut(s)	457
FspAI	RTGCGCAY	1 cut(s)	1917
FspBI	CTAG	6 cut(s)	1185, 1226, 1829, 2030, 2066, 2811
FspI	TGCGCA	1 cut(s)	1917
GlaI	GCGC	3 cut(s)	780, 1058, 1917
GsaI	CCCAGC	2 cut(s)	270, 2497
GsuI	CTGGAG	3 cut(s)	78, 359, 1482
HapII	CCGG	9 cut(s)	42, 174, 206, 546, 803, 1354, 2157, 2588, 2713
HhaI	GCGC	3 cut(s)	781, 1059, 1918
Hin6I	GCGC	3 cut(s)	779, 1057, 1916
HinP1I	GCGC	3 cut(s)	779, 1057, 1916
HpaII	CCGG	9 cut(s)	42, 174, 206, 546, 803, 1354, 2157, 2588, 2713
HphI	GGTGA	8 cut(s)	139, 205, 535, 1325, 1936, 2432, 2485, 2577
Hpy166II	GTNNAC	4 cut(s)	229, 999, 1126, 1927
Hpy8I	GTNNAC	4 cut(s)	229, 999, 1126, 1927
HpyCH4III	ACNGT	7 cut(s)	415, 627, 1516, 1963, 2200, 2473, 2554
HpyCH4IV	ACGT	4 cut(s)	89, 231, 398, 1735
HpyF3I	CTNAG	8 cut(s)	123, 456, 466, 983, 1254, 1708, 2043, 2505
HpySE526I	ACGT	4 cut(s)	89, 231, 398, 1735
HspAI	GCGC	3 cut(s)	779, 1057, 1916
Ksp22I	TGATCA	1 cut(s)	1887
Kzo9I	GATC	8 cut(s)	1139, 1372, 1639, 1788, 1887, 2344, 2574, 2643
LguI	GCTCTTC	2 cut(s)	1782, 2666
LmnI	GCTCC	6 cut(s)	207, 410, 452, 1040, 1624, 2840
Lsp1109I	GCAGC	7 cut(s)	151, 1118, 2023, 2612, 2663, 2773, 2830
LweI	GCATC	5 cut(s)	57, 1486, 1880, 2156, 2864
MabI	ACCWGGT	1 cut(s)	2356
MaeI	CTAG	6 cut(s)	1185, 1226, 1829, 2030, 2066, 2811
MaeII	ACGT	4 cut(s)	89, 231, 398, 1735
MaeIII	GTNAC	6 cut(s)	36, 508, 1477, 2413, 2420, 2759
MalI	GATC	8 cut(s)	1141, 1374, 1641, 1790, 1889, 2346, 2576, 2645
MboI	GATC	8 cut(s)	1139, 1372, 1639, 1788, 1887, 2344, 2574, 2643
MfeI	CAATTG	2 cut(s)	2425, 2619
MflI	RGATCY	2 cut(s)	1139, 1788
MhlI	GDGCHC	4 cut(s)	457, 1573, 2061, 2365
MlsI	TGGCCA	1 cut(s)	2128
MluNI	TGGCCA	1 cut(s)	2128
MlyI	GAGTC	4 cut(s)	773, 1607, 1948, 2576
MmeI	TCCRAC	6 cut(s)	130, 601, 921, 2202, 2590, 2898
Mox20I	TGGCCA	1 cut(s)	2128
Mph1103I	ATGCAT	1 cut(s)	1906
MroXI	GAANNNNTTC	1 cut(s)	660
MscI	TGGCCA	1 cut(s)	2128
MslI	CAYNNNNRTG	2 cut(s)	1004, 1443
Msp20I	TGGCCA	1 cut(s)	2128
MspA1I	CMGCKG	1 cut(s)	2654
MspI	CCGG	9 cut(s)	42, 174, 206, 546, 803, 1354, 2157, 2588, 2713
MunI	CAATTG	2 cut(s)	2425, 2619
MvaI	CCWGG	7 cut(s)	115, 666, 924, 1392, 1702, 2125, 2358
MvnI	CGCG	1 cut(s)	779
NciI	CCSGG	7 cut(s)	42, 43, 175, 206, 207, 803, 2713
NcoI	CCATGG	3 cut(s)	903, 1060, 2828
NdeII	GATC	8 cut(s)	1139, 1372, 1639, 1788, 1887, 2344, 2574, 2643
NlaIV	GGNNCC	6 cut(s)	203, 775, 1284, 1396, 1513, 2795
NmeAIII	GCCGAG	1 cut(s)	2476
NmuCI	GTSAC	2 cut(s)	1477, 2420
NsbI	TGCGCA	1 cut(s)	1917
NsiI	ATGCAT	1 cut(s)	1906
NspI	RCATGY	3 cut(s)	37, 162, 1904
NspV	TTCGAA	2 cut(s)	552, 2182
OliI	CACNNNNGTG	1 cut(s)	1004
PaeR7I	CTCGAG	1 cut(s)	2364
PceI	AGGCCT	2 cut(s)	134, 1827
PciI	ACATGT	1 cut(s)	158
PciSI	GCTCTTC	2 cut(s)	1782, 2666
PcsI	WCGNNNNNNNCGW	1 cut(s)	759
PdmI	GAANNNNTTC	1 cut(s)	660
PfeI	GAWTC	6 cut(s)	419, 487, 549, 979, 2239, 2775
PflFI	GACNNNGTC	1 cut(s)	1434
PfoI	TCCNGGA	2 cut(s)	922, 2711
Ple19I	CGATCG	1 cut(s)	2646
PleI	GAGTC	4 cut(s)	772, 1606, 1948, 2576
PpsI	GAGTC	4 cut(s)	772, 1606, 1948, 2576
PpuMI	RGGWCCY	1 cut(s)	2794
PscI	ACATGT	1 cut(s)	158
PsiI	TTATAA	2 cut(s)	720, 2693
Psp124BI	GAGCTC	1 cut(s)	457
Psp5II	RGGWCCY	1 cut(s)	2794
Psp6I	CCWGG	7 cut(s)	113, 664, 922, 1390, 1700, 2123, 2356
PspFI	CCCAGC	2 cut(s)	266, 2493
PspGI	CCWGG	7 cut(s)	113, 664, 922, 1390, 1700, 2123, 2356
PspN4I	GGNNCC	6 cut(s)	203, 775, 1284, 1396, 1513, 2795
PspPI	GGNCC	4 cut(s)	44, 737, 774, 2794
PspPPI	RGGWCCY	1 cut(s)	2794
PstI	CTGCAG	1 cut(s)	2791
PstNI	CAGNNNCTG	2 cut(s)	982, 2843
PsuI	RGATCY	2 cut(s)	1139, 1788
PsyI	GACNNNGTC	1 cut(s)	1434
PvuI	CGATCG	1 cut(s)	2646
PvuII	CAGCTG	1 cut(s)	2654
RsaI	GTAC	5 cut(s)	880, 1168, 1626, 1858, 2434
RsaNI	GTAC	5 cut(s)	879, 1167, 1625, 1857, 2433
RseI	CAYNNNNRTG	2 cut(s)	1004, 1443
SacI	GAGCTC	1 cut(s)	457
SapI	GCTCTTC	2 cut(s)	1782, 2666
Sau3AI	GATC	8 cut(s)	1139, 1372, 1639, 1788, 1887, 2344, 2574, 2643
Sau96I	GGNCC	4 cut(s)	44, 737, 774, 2794
SchI	GAGTC	4 cut(s)	773, 1607, 1948, 2576
SduI	GDGCHC	4 cut(s)	457, 1573, 2061, 2365
SexAI	ACCWGGT	1 cut(s)	2356
SfaNI	GCATC	5 cut(s)	57, 1486, 1880, 2156, 2864
SfcI	CTRYAG	4 cut(s)	1839, 2199, 2307, 2787
Sfr274I	CTCGAG	1 cut(s)	2364
SfuI	TTCGAA	2 cut(s)	552, 2182
SinI	GGWCC	3 cut(s)	737, 774, 2794
SlaI	CTCGAG	1 cut(s)	2364
SmaI	CCCGGG	2 cut(s)	43, 207
SmiMI	CAYNNNNRTG	2 cut(s)	1004, 1443
SmlI	CTYRAG	4 cut(s)	2013, 2364, 2384, 2927
SmoI	CTYRAG	4 cut(s)	2013, 2364, 2384, 2927
SpeI	ACTAGT	2 cut(s)	1184, 2029
SseBI	AGGCCT	2 cut(s)	134, 1827
SsiI	CCGC	6 cut(s)	191, 199, 222, 777, 1896, 2852
SspMI	CTAG	6 cut(s)	1185, 1226, 1829, 2030, 2066, 2811
SstI	GAGCTC	1 cut(s)	457
StuI	AGGCCT	2 cut(s)	134, 1827
StyI	CCWWGG	5 cut(s)	903, 1060, 1092, 2161, 2828
TaaI	ACNGT	7 cut(s)	415, 627, 1516, 1963, 2200, 2473, 2554
TaiI	ACGT	4 cut(s)	92, 234, 401, 1738
TaqII	GACCGA	2 cut(s)	1111, 1783
TatI	WGTACW	2 cut(s)	878, 1624
TauI	GCSGC	3 cut(s)	202, 224, 2855
TfiI	GAWTC	6 cut(s)	419, 487, 549, 979, 2239, 2775
TscAI	CASTG	6 cut(s)	418, 1305, 1482, 1671, 1980, 2355
TseFI	GTSAC	2 cut(s)	1477, 2420
TseI	GCWGC	7 cut(s)	164, 1106, 2036, 2600, 2651, 2786, 2843
Tsp45I	GTSAC	2 cut(s)	1477, 2420
TspGWI	ACGGA	1 cut(s)	2851
TspMI	CCCGGG	2 cut(s)	41, 205
TspRI	CASTG	6 cut(s)	418, 1305, 1482, 1671, 1980, 2355
Tth111I	GACNNNGTC	1 cut(s)	1434
VpaK11BI	GGWCC	3 cut(s)	737, 774, 2794
XagI	CCTNNNNNAGG	1 cut(s)	2809
XapI	RAATTY	3 cut(s)	854, 2018, 2116
XbaI	TCTAGA	1 cut(s)	1225
XceI	RCATGY	3 cut(s)	37, 162, 1904
XhoI	CTCGAG	1 cut(s)	2364
XmaI	CCCGGG	2 cut(s)	41, 205
XmiI	GTMKAC	1 cut(s)	1125
XmnI	GAANNNNTTC	1 cut(s)	660
XspI	CTAG	6 cut(s)	1185, 1226, 1829, 2030, 2066, 2811
Zsp2I	ATGCAT	1 cut(s)	1906

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.