Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0002025

Physical Location & Seq

Genomic Coordinates Forward (+)

54009769 .. 54014493

Gene Structure 4725 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000003888

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2889 bp

ATGAGTTGCATTTGCAGGGATTGCTTGGGTGGCATGTTACCCGGGCCGCGGCCACACTACCCCTCGATGCCCCGACACCCGAAAGGTACGTCTCCAGAAGCAACGAGGAAGGCCAGGGGTTCTGAGGTGAAGGCCTTGTTCTCTGTGGTTGGAATGACATGTGCCGGGTCTGTGGAGAAGGCGGTGAAGCGGCTCCCGGGGATACAGGGGGCCGCCGTGGACGTGTTGAACAACACAGCCCTTGTTCGCTACTATCCCAGCTTCGTCAATGAGGACAAAATATGTAAGGCTATTAAAGATGAGGGATTTGAAGCTAAACTGATTAAGCTGGAGATGATTGACAAAACCAGACAAGTATGCAGAATAAGCATAAGTGGAATGACTTGCACGTCTTGCTCCTCCACTGTTGAATCAGCTCTGCAATCAATTCATGGAGTGCAAGGAGCTCAGGTGGCCTTAGCAACTGAAGAAGCAGAGATTCATTATGACCCAAGCATTGTTACCTCCCATCAGCTATTGGAAACCATAGACACCACCGGATTCGGAGCCAAACTTATTAGTTTAGGGGAAGGCATTAGCAAGATAGAGCTTCAAGTTGATGGCATAAAGGTTGGACAGTCGATTAGAGCAATAGCAAAAACTCTTGAAGCACTTCCTGGGGTTCAAGACATAGAGACATTTCCTGAACTCAACAAGATTTCTATTTCTTATAAACCAGAGATGATAGGACCAAGAACCCTCATCGAAGCCATCGAGTCGTCAGGGTCCGCGCATTTCAAAGCAATGATATACCCGGAAGGAGAAAAAGACACTCATAGACAGAAGGAAATTAAGCAATACTATAAATTCTTCTTATGGAGTTTGTTTTGTACAATTCCTGTGTTTTTAACCTCCATGGTCTTGATGTATATTCCTGGAATTAAGAAGGTGTTGGATGTCAAAATTGTGAATAAGTTGAGTGTGGGTCAGATTCTGAGGTGGGAGTTGTCCACTCCTGTGCAGTTCATCATAGGCAAGAGGTTCTACATTGGAGCATACAAAGCATTGCGCCATGGTTCAGCTAACATGGATGTTTTGATTGCCTTGGGAACAAATGCAGCCTACTTCTACTCGGTCTACATAGTTTTAAGATCTGCAACCCATAAGGATTTCAAGGGTACAGATTTTTTTGAGACTAGTTCAATGCTTATTACATTCATCCTGTTAGGAAAGTATCTAGAGGTCTTGGCAAAAGGCAAGACATCTGAGGCCATTGCCAAGCTAATGGACTTGGCACCAGAAAAGGCAACACTGCTAACTTTGGGTGAGGAAGGAAATGTGATAAATGAACAAGAAATGGATAGCCGGTTGATACAAAAGAACGATCTGATTAAAGTTATTCCTGGTGCCAAAGTAGCTTGTGATGGTGTTGTTATGTGGGGACAAAGTCATGTGAATGAAAGCATGATAACTGGAGAAGCACGGCCAGTGACAAAAAAGAAGGATGATGCAGTTATTGGAGGCACCGTGAATGAGAATGGAGTGTTGCATATTAAGGCAACAAGAGTTGGGTCAGAGAGTGCTCTTTCACAGATTGTAAGGCTCGTCGAGTCTGCCCAGATGGCTAAAGCTCCTGTACAGAAGTTTGCTGATCGCATTTCCAAATACTTTGTGCCAGTGGTCATCACACTATCATTTTCGACTTGGCTTGCCTGGTTCTTAGGAGGGAAGTTCCACAGGTATCCAAGTTCTTGGATACCATCTTCAATCGATAGTTTTGAGCTTGCTCTTCAGTTTGGGATCTCGGTCATGGTCATTGCCTGCCCTTGTGCTTTAGGCCTAGCAACTCCTACTGCCGTTATGGTTGGTACAGGAGTTGGTGCATCTCAAGGTATACTGATCAAAGGCGGACATGCATTAGAGAGTGCGCATAAGGTGAGCTGCATTGTGTTTGACAAGACAGGGACTCTCACAGTTGGGAAGCCAGTGGTTGTTAGCACCAAAATCTTTAACAAAATGCTACCTCAAGAATTCTATGAACTAGTTGCTGCAACTGAGGTAAACAGTGAGCACCCACTAGCCAAAGCTATTGTGGAATATGCCAAGAAAATCAAACAAGATGAAGAGAATTCTACCTGGCCAGAAGCACAAAACTTCGCATCCCGTACCGGCCAAGGAGTGAAAGCAGTTGACATTAACATTGCGATTCCAGAGGAGGCTGAAGTTGCACTTGCACAAACTGAAGCATTGGCTCAAACTGGGATTTTAATAGCTGTAGATAACGAAATAGCAGGAATTATAGCCATATCTGATCCACTGAAACCTGGTGCTCGAGAAGTCATTTCCATACTTAAGTCGATGGGAGTTAGAAGTATCATGGTTACAGGTGACAATAGGGGTACAGCCAATTCTATTGCCAAGGAAACTGAAATTGAGACGGTGGTTGCAGAAGCAAAACCCCAGCACAAAGCTGAGAAAATAAAAGATTTGCAGGCTTCAGGCTACACCGTGGCAATGGTGGGAGATGGGATCAATGACTCACCGGCACTTGCGGCAGCAGATGTTGGAATGGCAATTGGTGCTGGCACAGACATTGCGATCGAGGCAGCTGACATTGTTCTTATGAAGAGCAACTTGGAAGATGTTATAACAGCCATTGACCTTTCCCGGAAAACCTTTTCCCGTATTCGTCTGAACTACATTTGGGCATTGGGTTACAATGTGCTTGGAATCCCGATAGCTGCAGGGGTCCTTTTTCCTTCCACTAGGTTCAGATTACCACCATGGATTGCAGGAGCTGCAATGGCCGCATCCTCCGTTAGTGTTGTATGCTGTTCTCTGTTGTTGAAGAACTACAAGAGACCCAAGAAGTTGGATATCCTTGAGATACATGAAGTAAGGGTTGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0008150 GO:0008324 GO:0009636 GO:0010035 GO:0010038 GO:0010273 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042221 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046688 GO:0046873 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0061687 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0097501 GO:0098655 GO:0098660 GO:0098662 GO:0098754 GO:0099131 GO:0099132 GO:1990169

KEGG Pathways

Metabolic & Signaling

ko01524 ko04016 map01524 map04016

Pfam Domains

Protein Families

E1-E2_ATPase HMA Hydrolase

Protein Analysis

963

Amino Acids

104.07

Weight (kDa)

8.0

Isoelectric Point (pI)

36.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMA	PF00403	47 - 104	5.1e-08	Heavy-metal-associated domain
HMA	PF00403	122 - 181	1.9e-12	Heavy-metal-associated domain
E1-E2_ATPase	PF00122	424 - 530	3.3e-25	P-type ATPase actuator domain
Hydrolase	PF00702	640 - 847	3.2e-35	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0002025)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G63440
fragaria_vesca	FvH4_5g02090 FvH4_5g02090 FvH4_5g02091 FvH4_5g02091 FvH4_5g02091 FvH4_5g02091
malus_domestica	MD06G1080800.v1.1 MD06G1080900.v1.1 MD14G1102600.v1.1
prunus_persica	Prupe.5G097900_v2.0.a1 Prupe.5G097900_v2.0.a1 Prupe.5G098000_v2.0.a1
pyrus_communis	pycom06g07780 pycom06g07790 pycom14g09890
rosa_chinensis	RchiOBHm_Chr7g0197781 RchiOBHm_Chr7g0198111 RchiOBHm_Chr7g0198141
rosa_laevigata	RLG00000003885 RLG00000003888 RLG00000019170
rosa_multiflora	Rmu_sc0000221.1_g000018 Rmu_sc0000221.1_g000030 Rmu_sc0006732.1_g000016
rosa_roxburghii	Rroxscaffold_3G00257940 Rroxscaffold_3G00257970 Rroxscaffold_3G00257990
rosa_rugosa	Rorug07G0045700 Rorug07G0045700 Rorug07G0045800 Rorug07G0045800 Rorug07G0045900
rosa_samantha	Rh7AG169100 Rh7AG169200 Rh7BG169900 Rh7BG172300 Rh7CG178600 Rh7CG178700 Rh7DG169200 Rh7DG171400 Rh7DG171800
rosa_wichuraiana	Rw7G014570 Rw7G014780 Rw7G014790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	711, 2627
AasI	GACNNNNNNGTC	1 cut(s)	388
Acc16I	TGCGCA	1 cut(s)	1908
AccB1I	GGYRCC	3 cut(s)	1273, 1385, 1502
AccI	GTMKAC	2 cut(s)	1116, 1873
AccII	CGCG	2 cut(s)	49, 770
AciI	CCGC	9 cut(s)	47, 49, 182, 190, 213, 768, 1887, 2531, 2787
AclWI	GGATC	3 cut(s)	1787, 2285, 2516
AcoI	YGGCCR	5 cut(s)	50, 1463, 2117, 2149, 2784
AcsI	RAATTY	3 cut(s)	845, 2009, 2107
AcuI	CTGAAG	5 cut(s)	486, 1754, 2220, 2241, 2460
AfaI	GTAC	7 cut(s)	88, 871, 1159, 1617, 1849, 2146, 2380
AfiI	CCNNNNNNNGG	1 cut(s)	48
AflII	CTTAAG	1 cut(s)	2330
AflIII	ACRYGT	2 cut(s)	158, 222
AhlI	ACTAGT	2 cut(s)	1175, 2020
AjiI	CACGTC	2 cut(s)	223, 390
AjnI	CCWGG	7 cut(s)	113, 655, 913, 1381, 1691, 2114, 2302
AjuI	GAANNNNNNNTTGG	2 cut(s)	2597, 2629
AleI	CACNNNNGTG	1 cut(s)	995
Alw21I	GWGCWC	4 cut(s)	448, 1564, 2052, 2311
Alw26I	GTCTC	5 cut(s)	96, 668, 1166, 2408, 2833
AlwI	GGATC	3 cut(s)	1787, 2285, 2516
AlwNI	CAGNNNCTG	2 cut(s)	973, 2777
Ama87I	CYCGRG	3 cut(s)	41, 196, 2310
ApeKI	GCWGC	7 cut(s)	1097, 1920, 2027, 2534, 2585, 2720, 2777
ApoI	RAATTY	3 cut(s)	845, 2009, 2107
ArsI	GACNNNNNNTTYG	2 cut(s)	74, 106
Asp700I	GAANNNNTTC	1 cut(s)	651
AspLEI	GCGC	3 cut(s)	772, 1050, 1909
AspS9I	GGNCC	5 cut(s)	44, 210, 728, 765, 2728
AsuC2I	CCSGG	7 cut(s)	42, 43, 166, 197, 198, 794, 2647
AsuHPI	GGTGA	6 cut(s)	139, 196, 1316, 1927, 2378, 2511
AvaI	CYCGRG	3 cut(s)	41, 196, 2310
AvaII	GGWCC	3 cut(s)	728, 765, 2728
BaeI	ACNNNNGTAYC	2 cut(s)	1839, 1872
BalI	TGGCCA	1 cut(s)	2119
BanI	GGYRCC	3 cut(s)	1273, 1385, 1502
BanII	GRGCYC	1 cut(s)	448
Bbv12I	GWGCWC	4 cut(s)	448, 1564, 2052, 2311
BbvI	GCAGC	7 cut(s)	1109, 1907, 2014, 2546, 2597, 2707, 2764
BccI	CCATC	8 cut(s)	516, 593, 758, 1397, 1594, 1747, 2332, 2498
BceAI	ACGGC	3 cut(s)	200, 1478, 1820
BciT130I	CCWGG	7 cut(s)	115, 657, 915, 1383, 1693, 2116, 2304
BciVI	GTATCC	3 cut(s)	195, 1728, 1731
BclI	TGATCA	1 cut(s)	1878
BcnI	CCSGG	7 cut(s)	42, 43, 166, 197, 198, 794, 2647
BcoDI	GTCTC	5 cut(s)	96, 668, 1166, 2408, 2833
BcuI	ACTAGT	2 cut(s)	1175, 2020
BfaI	CTAG	6 cut(s)	1176, 1217, 1820, 2021, 2057, 2745
BfmI	CTRYAG	2 cut(s)	2253, 2721
BfrI	CTTAAG	1 cut(s)	2330
BfuI	GTATCC	3 cut(s)	195, 1728, 1731
BglI	GCCNNNNNGGC	1 cut(s)	1601
BglII	AGATCT	1 cut(s)	1130
Bme18I	GGWCC	3 cut(s)	728, 765, 2728
BmeT110I	CYCGRG	3 cut(s)	41, 196, 2310
BmgBI	CACGTC	2 cut(s)	223, 390
BmgT120I	GGNCC	5 cut(s)	44, 210, 728, 765, 2728
BmiI	GGNNCC	8 cut(s)	194, 211, 547, 766, 1275, 1387, 1504, 2729
BmrI	ACTGGG	1 cut(s)	2247
BmsI	GCATC	5 cut(s)	57, 1477, 1871, 2147, 2798
BmuI	ACTGGG	1 cut(s)	2247
BpmI	CTGGAG	3 cut(s)	78, 350, 1473
Bpu10I	CCTNAGC	2 cut(s)	447, 457
BpuEI	CTTGAG	3 cut(s)	1851, 1989, 2882
BpuMI	CCSGG	7 cut(s)	42, 43, 166, 197, 198, 794, 2647
Bsa29I	ATCGAT	1 cut(s)	1749
BsaI	GGTCTC	1 cut(s)	2833
BsaWI	WCCGGW	1 cut(s)	536
BsaXI	ACNNNNNCTCC	4 cut(s)	435, 465, 1491, 1521
Bsc4I	CCNNNNNNNGG	1 cut(s)	48
Bse118I	RCCGGY	3 cut(s)	1344, 2147, 2521
Bse1I	ACTGG	5 cut(s)	1456, 1466, 1655, 1964, 2242
Bse3DI	GCAATG	8 cut(s)	789, 1043, 1251, 1794, 2178, 2499, 2571, 2787
BseBI	CCWGG	7 cut(s)	115, 657, 915, 1383, 1693, 2116, 2304
BseCI	ATCGAT	1 cut(s)	1749
BseGI	GGATG	6 cut(s)	940, 1075, 1197, 1489, 2138, 2789
BseLI	CCNNNNNNNGG	1 cut(s)	48
BseMI	GCAATG	8 cut(s)	789, 1043, 1251, 1794, 2178, 2499, 2571, 2787
BseMII	CTCAG	6 cut(s)	114, 461, 965, 1236, 2025, 2442
BseNI	ACTGG	5 cut(s)	1456, 1466, 1655, 1964, 2242
BseRI	GAGGAG	2 cut(s)	388, 2207
BseXI	GCAGC	7 cut(s)	1109, 1907, 2014, 2546, 2597, 2707, 2764
BseYI	CCCAGC	2 cut(s)	257, 2439
BsgI	GTGCAG	1 cut(s)	1019
Bsh1236I	CGCG	2 cut(s)	49, 770
Bsh1285I	CGRYCG	1 cut(s)	2580
BshNI	GGYRCC	3 cut(s)	1273, 1385, 1502
BshVI	ATCGAT	1 cut(s)	1749
BsiEI	CGRYCG	1 cut(s)	2580
BsiHKAI	GWGCWC	4 cut(s)	448, 1564, 2052, 2311
BsiHKCI	CYCGRG	3 cut(s)	41, 196, 2310
BsiSI	CCGG	9 cut(s)	42, 165, 197, 537, 794, 1345, 2148, 2522, 2647
BslFI	GGGAC	2 cut(s)	1434, 1957
BslI	CCNNNNNNNGG	1 cut(s)	48
BsmAI	GTCTC	5 cut(s)	96, 668, 1166, 2408, 2833
BsmBI	CGTCTC	2 cut(s)	96, 2408
BsmFI	GGGAC	2 cut(s)	1434, 1957
Bso31I	GGTCTC	1 cut(s)	2833
BsoBI	CYCGRG	3 cut(s)	41, 196, 2310
Bsp1286I	GDGCHC	4 cut(s)	448, 1564, 2052, 2311
Bsp1407I	TGTACA	2 cut(s)	869, 1615
Bsp143I	GATC	8 cut(s)	1130, 1363, 1630, 1779, 1878, 2290, 2508, 2577
Bsp19I	CCATGG	3 cut(s)	894, 1051, 2762
BspACI	CCGC	9 cut(s)	47, 49, 182, 190, 213, 768, 1887, 2531, 2787
BspCNI	CTCAG	6 cut(s)	115, 460, 966, 1237, 2026, 2443
BspDI	ATCGAT	1 cut(s)	1749
BspFNI	CGCG	2 cut(s)	49, 770
BspLI	GGNNCC	8 cut(s)	194, 211, 547, 766, 1275, 1387, 1504, 2729
BspMAI	CTGCAG	1 cut(s)	2725
BspPI	GGATC	3 cut(s)	1787, 2285, 2516
BspQI	GCTCTTC	2 cut(s)	1773, 2600
BspT107I	GGYRCC	3 cut(s)	1273, 1385, 1502
BspTI	CTTAAG	1 cut(s)	2330
BspTNI	GGTCTC	1 cut(s)	2833
BsrDI	GCAATG	8 cut(s)	789, 1043, 1251, 1794, 2178, 2499, 2571, 2787
BsrFI	RCCGGY	3 cut(s)	1344, 2147, 2521
BsrGI	TGTACA	2 cut(s)	869, 1615
BsrI	ACTGG	5 cut(s)	1456, 1466, 1655, 1964, 2242
BssAI	RCCGGY	3 cut(s)	1344, 2147, 2521
BssMI	GATC	8 cut(s)	1130, 1363, 1630, 1779, 1878, 2290, 2508, 2577
BssNAI	GTATAC	1 cut(s)	1874
BssT1I	CCWWGG	6 cut(s)	894, 1051, 1083, 2152, 2397, 2762
Bst1107I	GTATAC	1 cut(s)	1874
Bst2UI	CCWGG	7 cut(s)	115, 657, 915, 1383, 1693, 2116, 2304
Bst4CI	ACNGT	7 cut(s)	406, 618, 1507, 1954, 2045, 2419, 2488
Bst6I	CTCTTC	3 cut(s)	1773, 2097, 2600
BstAFI	CTTAAG	1 cut(s)	2330
BstAPI	GCANNNNNTGC	4 cut(s)	21, 393, 2558, 2777
BstAUI	TGTACA	2 cut(s)	869, 1615
BstC8I	GCNNGC	5 cut(s)	1689, 1764, 1801, 2472, 2563
BstDEI	CTNAG	8 cut(s)	123, 447, 457, 974, 1245, 1699, 2034, 2451
BstDSI	CCRYGG	6 cut(s)	47, 216, 894, 1051, 2487, 2762
BstF5I	GGATG	6 cut(s)	940, 1075, 1197, 1489, 2138, 2789
BstFNI	CGCG	2 cut(s)	49, 770
BstHHI	GCGC	3 cut(s)	772, 1050, 1909
BstKTI	GATC	8 cut(s)	1133, 1366, 1633, 1782, 1881, 2293, 2511, 2580
BstMAI	GTCTC	5 cut(s)	96, 668, 1166, 2408, 2833
BstMBI	GATC	8 cut(s)	1130, 1363, 1630, 1779, 1878, 2290, 2508, 2577
BstMCI	CGRYCG	1 cut(s)	2580
BstNI	CCWGG	7 cut(s)	115, 657, 915, 1383, 1693, 2116, 2304
BstNSI	RCATGY	3 cut(s)	37, 162, 1895
BstSFI	CTRYAG	2 cut(s)	2253, 2721
BstUI	CGCG	2 cut(s)	49, 770
BstV1I	GCAGC	7 cut(s)	1109, 1907, 2014, 2546, 2597, 2707, 2764
BstX2I	RGATCY	2 cut(s)	1130, 1779
BstXI	CCANNNNNNTGG	3 cut(s)	1264, 1731, 2851
BstYI	RGATCY	2 cut(s)	1130, 1779
BstZ17I	GTATAC	1 cut(s)	1874
Bsu15I	ATCGAT	1 cut(s)	1749
BsuI	GTATCC	3 cut(s)	195, 1728, 1731
BsuTUI	ATCGAT	1 cut(s)	1749
BtgI	CCRYGG	6 cut(s)	47, 216, 894, 1051, 2487, 2762
BtrI	CACGTC	2 cut(s)	223, 390
BtsCI	GGATG	6 cut(s)	940, 1075, 1197, 1489, 2138, 2789
BtsI	GCAGTG	1 cut(s)	1289
BtsIMutI	CAGTG	7 cut(s)	402, 1289, 1473, 1662, 1971, 2050, 2294
Cac8I	GCNNGC	5 cut(s)	1689, 1764, 1801, 2472, 2563
CaiI	CAGNNNCTG	2 cut(s)	973, 2777
CfoI	GCGC	3 cut(s)	772, 1050, 1909
Cfr10I	RCCGGY	3 cut(s)	1344, 2147, 2521
Cfr13I	GGNCC	5 cut(s)	44, 210, 728, 765, 2728
Cfr42I	CCGCGG	1 cut(s)	50
Cfr9I	CCCGGG	2 cut(s)	41, 196
ClaI	ATCGAT	1 cut(s)	1749
CsiI	ACCWGGT	1 cut(s)	2302
Csp6I	GTAC	7 cut(s)	87, 870, 1158, 1616, 1848, 2145, 2379
CviQI	GTAC	7 cut(s)	87, 870, 1158, 1616, 1848, 2145, 2379
DdeI	CTNAG	8 cut(s)	123, 447, 457, 974, 1245, 1699, 2034, 2451
DpnI	GATC	8 cut(s)	1132, 1365, 1632, 1781, 1880, 2292, 2510, 2579
DpnII	GATC	8 cut(s)	1130, 1363, 1630, 1779, 1878, 2290, 2508, 2577
DrdI	GACNNNNNNGTC	1 cut(s)	388
DseDI	GACNNNNNNGTC	1 cut(s)	388
EaeI	YGGCCR	5 cut(s)	50, 1463, 2117, 2149, 2784
Eam1104I	CTCTTC	3 cut(s)	1773, 2097, 2600
EarI	CTCTTC	3 cut(s)	1773, 2097, 2600
EciI	GGCGGA	1 cut(s)	1902
Ecl136II	GAGCTC	1 cut(s)	446
Eco130I	CCWWGG	6 cut(s)	894, 1051, 1083, 2152, 2397, 2762
Eco147I	AGGCCT	2 cut(s)	134, 1818
Eco24I	GRGCYC	1 cut(s)	448
Eco31I	GGTCTC	1 cut(s)	2833
Eco32I	GATATC	1 cut(s)	2857
Eco47I	GGWCC	3 cut(s)	728, 765, 2728
Eco53kI	GAGCTC	1 cut(s)	446
Eco57I	CTGAAG	5 cut(s)	486, 1754, 2220, 2241, 2460
Eco88I	CYCGRG	3 cut(s)	41, 196, 2310
EcoICRI	GAGCTC	1 cut(s)	446
EcoO109I	RGGNCCY	1 cut(s)	2728
EcoRI	GAATTC	2 cut(s)	2009, 2107
EcoRII	CCWGG	7 cut(s)	113, 655, 913, 1381, 1691, 2114, 2302
EcoRV	GATATC	1 cut(s)	2857
EcoT14I	CCWWGG	6 cut(s)	894, 1051, 1083, 2152, 2397, 2762
EcoT22I	ATGCAT	1 cut(s)	1897
EcoT38I	GRGCYC	1 cut(s)	448
ErhI	CCWWGG	6 cut(s)	894, 1051, 1083, 2152, 2397, 2762
Esp3I	CGTCTC	2 cut(s)	96, 2408
FalI	AAGNNNNNCTT	4 cut(s)	2193, 2225, 2597, 2629
FaqI	GGGAC	2 cut(s)	1434, 1957
FbaI	TGATCA	1 cut(s)	1878
FblI	GTMKAC	2 cut(s)	1116, 1873
FokI	GGATG	6 cut(s)	947, 1082, 1184, 1496, 2125, 2776
FriOI	GRGCYC	1 cut(s)	448
FspAI	RTGCGCAY	1 cut(s)	1908
FspBI	CTAG	6 cut(s)	1176, 1217, 1820, 2021, 2057, 2745
FspI	TGCGCA	1 cut(s)	1908
GlaI	GCGC	3 cut(s)	771, 1049, 1908
GsaI	CCCAGC	2 cut(s)	261, 2443
GsuI	CTGGAG	3 cut(s)	78, 350, 1473
HapII	CCGG	9 cut(s)	42, 165, 197, 537, 794, 1345, 2148, 2522, 2647
HhaI	GCGC	3 cut(s)	772, 1050, 1909
Hin6I	GCGC	3 cut(s)	770, 1048, 1907
HinP1I	GCGC	3 cut(s)	770, 1048, 1907
HincII	GTYRAC	1 cut(s)	2170
HindII	GTYRAC	1 cut(s)	2170
HpaII	CCGG	9 cut(s)	42, 165, 197, 537, 794, 1345, 2148, 2522, 2647
HphI	GGTGA	6 cut(s)	139, 196, 1316, 1927, 2378, 2511
Hpy166II	GTNNAC	6 cut(s)	220, 990, 1117, 1874, 2041, 2170
Hpy8I	GTNNAC	6 cut(s)	220, 990, 1117, 1874, 2041, 2170
Hpy99I	CGWCG	1 cut(s)	1589
HpyCH4III	ACNGT	7 cut(s)	406, 618, 1507, 1954, 2045, 2419, 2488
HpyCH4IV	ACGT	3 cut(s)	89, 222, 389
HpyF3I	CTNAG	8 cut(s)	123, 447, 457, 974, 1245, 1699, 2034, 2451
HpySE526I	ACGT	3 cut(s)	89, 222, 389
HspAI	GCGC	3 cut(s)	770, 1048, 1907
Ksp22I	TGATCA	1 cut(s)	1878
KspI	CCGCGG	1 cut(s)	50
Kzo9I	GATC	8 cut(s)	1130, 1363, 1630, 1779, 1878, 2290, 2508, 2577
LguI	GCTCTTC	2 cut(s)	1773, 2600
LmnI	GCTCC	7 cut(s)	198, 401, 443, 545, 1031, 1615, 2774
Lsp1109I	GCAGC	7 cut(s)	1109, 1907, 2014, 2546, 2597, 2707, 2764
LweI	GCATC	5 cut(s)	57, 1477, 1871, 2147, 2798
MabI	ACCWGGT	1 cut(s)	2302
MaeI	CTAG	6 cut(s)	1176, 1217, 1820, 2021, 2057, 2745
MaeII	ACGT	3 cut(s)	89, 222, 389
MaeIII	GTNAC	6 cut(s)	36, 499, 1468, 2359, 2366, 2693
MalI	GATC	8 cut(s)	1132, 1365, 1632, 1781, 1880, 2292, 2510, 2579
MboI	GATC	8 cut(s)	1130, 1363, 1630, 1779, 1878, 2290, 2508, 2577
MboII	GAAGA	8 cut(s)	479, 841, 1734, 1760, 2114, 2617, 2630, 2839
MfeI	CAATTG	1 cut(s)	2553
MflI	RGATCY	2 cut(s)	1130, 1779
MhlI	GDGCHC	4 cut(s)	448, 1564, 2052, 2311
MlsI	TGGCCA	1 cut(s)	2119
MluNI	TGGCCA	1 cut(s)	2119
MlyI	GAGTC	4 cut(s)	764, 1598, 1939, 2510
MmeI	TCCRAC	5 cut(s)	130, 592, 912, 2524, 2832
Mox20I	TGGCCA	1 cut(s)	2119
Mph1103I	ATGCAT	1 cut(s)	1897
MroXI	GAANNNNTTC	1 cut(s)	651
MscI	TGGCCA	1 cut(s)	2119
MslI	CAYNNNNRTG	2 cut(s)	995, 1434
Msp20I	TGGCCA	1 cut(s)	2119
MspA1I	CMGCKG	2 cut(s)	49, 2588
MspCI	CTTAAG	1 cut(s)	2330
MspI	CCGG	9 cut(s)	42, 165, 197, 537, 794, 1345, 2148, 2522, 2647
MunI	CAATTG	1 cut(s)	2553
MvaI	CCWGG	7 cut(s)	115, 657, 915, 1383, 1693, 2116, 2304
MvnI	CGCG	2 cut(s)	49, 770
NciI	CCSGG	7 cut(s)	42, 43, 166, 197, 198, 794, 2647
NcoI	CCATGG	3 cut(s)	894, 1051, 2762
NdeII	GATC	8 cut(s)	1130, 1363, 1630, 1779, 1878, 2290, 2508, 2577
NlaIV	GGNNCC	8 cut(s)	194, 211, 547, 766, 1275, 1387, 1504, 2729
NmuCI	GTSAC	2 cut(s)	1468, 2366
NsbI	TGCGCA	1 cut(s)	1908
NsiI	ATGCAT	1 cut(s)	1897
NspI	RCATGY	3 cut(s)	37, 162, 1895
OliI	CACNNNNGTG	1 cut(s)	995
PaeR7I	CTCGAG	1 cut(s)	2310
PceI	AGGCCT	2 cut(s)	134, 1818
PciI	ACATGT	1 cut(s)	158
PciSI	GCTCTTC	2 cut(s)	1773, 2600
PcsI	WCGNNNNNNNCGW	1 cut(s)	750
PdmI	GAANNNNTTC	1 cut(s)	651
PfeI	GAWTC	6 cut(s)	410, 478, 540, 970, 2185, 2709
PflFI	GACNNNGTC	1 cut(s)	1425
PfoI	TCCNGGA	2 cut(s)	913, 2645
Ple19I	CGATCG	1 cut(s)	2580
PleI	GAGTC	4 cut(s)	763, 1597, 1939, 2510
PpsI	GAGTC	4 cut(s)	763, 1597, 1939, 2510
PpuMI	RGGWCCY	1 cut(s)	2728
PscI	ACATGT	1 cut(s)	158
PsiI	TTATAA	2 cut(s)	711, 2627
Psp124BI	GAGCTC	1 cut(s)	448
Psp5II	RGGWCCY	1 cut(s)	2728
Psp6I	CCWGG	7 cut(s)	113, 655, 913, 1381, 1691, 2114, 2302
PspFI	CCCAGC	2 cut(s)	257, 2439
PspGI	CCWGG	7 cut(s)	113, 655, 913, 1381, 1691, 2114, 2302
PspN4I	GGNNCC	8 cut(s)	194, 211, 547, 766, 1275, 1387, 1504, 2729
PspPI	GGNCC	5 cut(s)	44, 210, 728, 765, 2728
PspPPI	RGGWCCY	1 cut(s)	2728
PstI	CTGCAG	1 cut(s)	2725
PstNI	CAGNNNCTG	2 cut(s)	973, 2777
PsuI	RGATCY	2 cut(s)	1130, 1779
PsyI	GACNNNGTC	1 cut(s)	1425
PvuI	CGATCG	1 cut(s)	2580
PvuII	CAGCTG	1 cut(s)	2588
RsaI	GTAC	7 cut(s)	88, 871, 1159, 1617, 1849, 2146, 2380
RsaNI	GTAC	7 cut(s)	87, 870, 1158, 1616, 1848, 2145, 2379
RseI	CAYNNNNRTG	2 cut(s)	995, 1434
SacI	GAGCTC	1 cut(s)	448
SacII	CCGCGG	1 cut(s)	50
SapI	GCTCTTC	2 cut(s)	1773, 2600
Sau3AI	GATC	8 cut(s)	1130, 1363, 1630, 1779, 1878, 2290, 2508, 2577
Sau96I	GGNCC	5 cut(s)	44, 210, 728, 765, 2728
SchI	GAGTC	4 cut(s)	764, 1598, 1939, 2510
SduI	GDGCHC	4 cut(s)	448, 1564, 2052, 2311
SexAI	ACCWGGT	1 cut(s)	2302
SfaNI	GCATC	5 cut(s)	57, 1477, 1871, 2147, 2798
SfcI	CTRYAG	2 cut(s)	2253, 2721
Sfr274I	CTCGAG	1 cut(s)	2310
Sfr303I	CCGCGG	1 cut(s)	50
SgrBI	CCGCGG	1 cut(s)	50
SinI	GGWCC	3 cut(s)	728, 765, 2728
SlaI	CTCGAG	1 cut(s)	2310
SmaI	CCCGGG	2 cut(s)	43, 198
SmiMI	CAYNNNNRTG	2 cut(s)	995, 1434
SmlI	CTYRAG	5 cut(s)	1866, 2004, 2310, 2330, 2861
SmoI	CTYRAG	5 cut(s)	1866, 2004, 2310, 2330, 2861
SpeI	ACTAGT	2 cut(s)	1175, 2020
SseBI	AGGCCT	2 cut(s)	134, 1818
SsiI	CCGC	9 cut(s)	47, 49, 182, 190, 213, 768, 1887, 2531, 2787
SspMI	CTAG	6 cut(s)	1176, 1217, 1820, 2021, 2057, 2745
SstI	GAGCTC	1 cut(s)	448
StuI	AGGCCT	2 cut(s)	134, 1818
StyI	CCWWGG	6 cut(s)	894, 1051, 1083, 2152, 2397, 2762
TaaI	ACNGT	7 cut(s)	406, 618, 1507, 1954, 2045, 2419, 2488
TaiI	ACGT	3 cut(s)	92, 225, 392
TaqII	GACCGA	2 cut(s)	1102, 1774
TatI	WGTACW	2 cut(s)	869, 1615
TauI	GCSGC	6 cut(s)	49, 52, 193, 215, 2534, 2789
TfiI	GAWTC	6 cut(s)	410, 478, 540, 970, 2185, 2709
TscAI	CASTG	7 cut(s)	409, 1296, 1473, 1662, 1971, 2050, 2301
TseFI	GTSAC	2 cut(s)	1468, 2366
TseI	GCWGC	7 cut(s)	1097, 1920, 2027, 2534, 2585, 2720, 2777
Tsp45I	GTSAC	2 cut(s)	1468, 2366
TspGWI	ACGGA	1 cut(s)	2785
TspMI	CCCGGG	2 cut(s)	41, 196
TspRI	CASTG	7 cut(s)	409, 1296, 1473, 1662, 1971, 2050, 2301
Tth111I	GACNNNGTC	1 cut(s)	1425
Vha464I	CTTAAG	1 cut(s)	2330
VpaK11BI	GGWCC	3 cut(s)	728, 765, 2728
XapI	RAATTY	3 cut(s)	845, 2009, 2107
XbaI	TCTAGA	1 cut(s)	1216
XceI	RCATGY	3 cut(s)	37, 162, 1895
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2068
XhoI	CTCGAG	1 cut(s)	2310
XmaI	CCCGGG	2 cut(s)	41, 196
XmiI	GTMKAC	2 cut(s)	1116, 1873
XmnI	GAANNNNTTC	1 cut(s)	651
XspI	CTAG	6 cut(s)	1176, 1217, 1820, 2021, 2057, 2745
Zsp2I	ATGCAT	1 cut(s)	1897

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.