Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr6

Orthogroup ID

OG0002025

Physical Location & Seq

Genomic Coordinates Forward (+)

12750489 .. 12764671

Gene Structure 14183 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom06g07790.4

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3765 bp

ATGAAACAAGTTTTTATTACCAACACACATAATGTCCTCGGAAAAAGTCTCAAGTTTCGTCGGAAATGCACTTTTGTTTGGCAAGAAGAATTGATGCTAGTTGTATTAACGATGAGTAAAGTTCTTATCAGAAATAGAATGGTTATTTCCGAAGATTATCCAAAAGAGCAAATGGATGAATTGTATGGTTGTTTTAAGGGAACTGGATACACATACACGTGTTCTGAAGAAGAGATGAGGGCTGCTTTTGAACTACATATTAAAACAAGATATTACAAGACTATTGGGGAGAGATACATTAACTGCCCATCAAATGTATCAAAAGATGTTTGGTGTAAAATGGTTGAAAAAAGGGAGACGCATGAATGGAAGAGCTTGATCGATAGTGGAATTGAACCTAGCCCGATTGATTGTTTCAAAAAATTCCATGTGAGTAAGCGTAAGAGTGAAGGTGAAAAAACTTGGGCAAGTGAAAAGGCTAAAGAACTTTATGAAAAAATGGACAACAAAAAGAATACTGAGGTGATTGGTGGCCCAAGTCATGGTCGTCTTCTTGGTGTAGGTGCTGGCTTTAGTACTAAAGATGCATATCCCTCAAATAGCCAAAGTTGTAATAAACGTATGTGCTTAGAACGTGATGAAAGTCATGAGAAATTGAAGGACCAAGTCAACACTTTAACAGATAAGTTGAATTCTTTGCAAGAATTAGTTGAAAGACTGATGCCAAGCAACTCAAATAATTATGCGCGCTCAGGACCATCTGCTTCTATGGGAAATTTCAACAACTTCCCAGAAAAGAAAACCCTATTAACTAAATTTGCTAGAATGGGTTGCATTTGCAATGACTGCTGGGGTGACATGTCACCCCGGCCGCGCTATCCGTCGATGCATAAGCACCCGAAAGGTACGTCGCCGGAAGCAAGGCATGTAAATGGCTCTGAGGTGAAGGCCTTGTTCTCTGTGGTTGGCATCACATGTTCTGCATGCGCCGGATCGATTGAAAAGGCGGTCAAGCGGCTTCCGGGGATCCGTGAGGCTGCCGTCGATGTGTTGAATAATACTGTCCATGTTCTCTACTATCCAAGCTTGGTTACTGAGGAAAAAATACGCGGGACTATGGAAGATGCGGGATTTGAAGCCAAATTGATCAAAGAGGAGACAATTAATGGCAAATTAAGACAAGTATGCAGAATAAGCATAAATGGAATGACTTGTACCTCTTGCTCCTCCACTGTTGAATCTGCCCTCCAAGCAGTTCATGGTGTACAAAGAGCTCAAGTGGCCTTAGCAACTGAAGAAGCAGAAGTTCATTACGATCCAAAGATTGTAAGCTACAATCAGCTGTCGGAAATCGTCGAAAACACCGGATTCGAAGCCAAACTTATTAGTCTAGGGGACGACATAAGCAAGATTGAGCTCAAAGTTGACGGTATAAAGACTGAACAAAGCATTAGAGCAATAACAAAATCCCTTGAGGCACTTCCTGGGGTTCAAGACATAGAGACATTCCCCGAACTCAACAAAATTTCGGTTTGTTATAAACAAGATGTGATAGGACCAAGAACTTTCATCGAAGTGATCGAATCATCAGGGTCCGCGCATTTCAAAGCAATGATATACCCTGAAGGGGGGAGAGACACTCATAGAAAGGAGGAAGTTAAGCAGTACTATAAATACTTTTTATGGAGTTTATTTTTCGCAATTCCCGTGTTTTTAACCTCCATGGTGTTGATGTATATCCCAGGAATTAAGAAGGTGCTTGATGTTAGAATTGTGAATAAGTTGACAGTTGGTCAGATTCTGAGGTGGGAGCTGTCCACTCCAGTGCAGTTCATCATAGGCAGGAGGTTCTACATTGGATCATACAAAGCGTTGCGCAACGGTTCTGCTAATATGGATGTTTTGATTGCCTTGGGAACCAATGCAGCCTATTTCTATTCAGTCTATATAGTTTTAAGATCTGCAATCTCTAGAGATTTCAAGGGTACAGATTTCTTTGAGACTAGCTCAATGCTTATTGCATTTATCTTGTTAGGCAAGTATCTGGAGGTTTTGGCAAAGGGGAAGACATCTGATGCCATTGCCAAGCTTATGAACTTGGCGCCCGAGACAGCAACGTTGCTGAGTTTGGATGAGGATGGAAATGTGGTAAACGAACAAGAAATCGATAGTAGGTTGATACAAAAGAATGATGTGATTAAAATCATTCCGGGTGCTAAAGTTGCCTGTGATGGTTTTGTTGTATGGGGGCAGAGCCATGTGAATGAAAGCATGATAACAGGAGAAGCACTGCCGGTGGGTAAAAATAAGGGTGATGCTGTCATTGGAGGCACTGTGAATGTGAATGGAGTGTTGCATATTAAGGCAACAAGGGTTGGATCCGAGAGTGCTCTTTCGCAGATTGTTCGACTTGTTGAATCTGCGCAAATGGCCAAAGCTCCTGTACAAAAGTTTGCTGATCGCATTTCGAAACACTTTGTGCCAACGGTTATCATTCTTTCATTTTCGACGTGGCTTGCCTGGTTTTTATCAGGGAAGTTCCATAGCTACCCACATTCTTGGATACCATCTTCTATCGACAGTTTTGAGCTTGCGCTTCAGTTCGGGATCTCAGTCATGGTCATAGCTTGCCCTTGTGCTTTAGGCCTAGCAACTCCTACTGCCGTTATGGTTGGTACCGGAGTTGGTGCATCTCAAGGTATACTGATCAAAGGGGGCCAAGCATTAGAGAACGCACATAAGGTGAACTGCATTGTTTTCGACAAGACGGGGACTCTCACAGTTGGGAAACCAGTGGTTGTAAACACAAAAATCTTCAATAATATGCTCCCTCATGAATTTTATGAACTTGTTGCAGCAACTGAGGTTAACAGTGAGCATCCACTAGCCAAGGCTATTGTAGAGCATGTCAAGAAATTCAGAGAAGGTGAAGACAATCCTTCCTGGCCTGAAGCACAAAATTTTGCATCCATTACCGGCCAAGGATTGAAGGCAGTTGTTCGGGACAAGGAGATAATCGTAGGCAACAAGAGTTTAATGTTGGATTCTAACATTGCCATTCCGGTGGAGGCTGAAGCTGCACTAGCAGAAGCTGAAGCATTAGCTCAAACCGGGATTCTAATAGCTATAGACAGAGAAGTAGCAGGAGTTGTAACCATATCCGATCCACTGAAACCCCGCGCCAAAGAAGTCATTTCCATACTCAAGTCTATGGGAGTTAAAAGTATCATGGTTACAGGTGACAACTGGGGCACAGCCAATTCCATTGCCAAGGAAACTGAAATTGAAACCGTGATCGCAGAAGCCAAACCCGAACATAAAGCTGAGAAAGTGAAGGATTTGCAGGCTACGGGGTACGTTGTTGCAATGGTGGGGGATGGGATCAACGACTCACCGGCACTTGTGGCAGCAGATGTAGGAATGGCAATTGGTGCTGGCACAGATATTGCTATTGAAGCAGCTGACATTGTTCTTATGAAGAGCAACTTGGAAGATGTGATAACAGCTATTGACCTTTCCAGGAAAACCTTCTCTCGCATTCGTTTGAACTATATCTGGGCGTTGGGATATAATGTGCTCGGAATCCCAGTAGCTGCGGGAGCCCTTTTTCCATCCACCAGGTTTAGATTACCGCCATGGATTGCCGGAGCTGCAATGGCTGCGTCCTCTGTTAGCGTCGTTTGCTGCTCTTTGTTGTTGAAGAACTACAAGAGACCCAAGAACTTGGATGTCCTTGAGATACATGAAGTAAGAATCGAACCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0008150 GO:0008324 GO:0009636 GO:0010035 GO:0010038 GO:0010273 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042221 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046688 GO:0046873 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0061687 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0097501 GO:0098655 GO:0098660 GO:0098662 GO:0098754 GO:0099131 GO:0099132 GO:1990169

KEGG Pathways

Metabolic & Signaling

ko01524 ko04016 map01524 map04016

Pfam Domains

Protein Families

E1-E2_ATPase HMA Hydrolase

Protein Analysis

1255

Amino Acids

138.03

Weight (kDa)

8.0

Isoelectric Point (pI)

36.79

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002025)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G63440
fragaria_vesca	FvH4_5g02090 FvH4_5g02090 FvH4_5g02091 FvH4_5g02091 FvH4_5g02091 FvH4_5g02091
malus_domestica	MD06G1080800.v1.1 MD06G1080900.v1.1 MD14G1102600.v1.1
prunus_persica	Prupe.5G097900_v2.0.a1 Prupe.5G097900_v2.0.a1 Prupe.5G098000_v2.0.a1
pyrus_communis	pycom06g07780 pycom06g07790 pycom14g09890
rosa_chinensis	RchiOBHm_Chr7g0197781 RchiOBHm_Chr7g0198111 RchiOBHm_Chr7g0198141
rosa_laevigata	RLG00000003885 RLG00000003888 RLG00000019170
rosa_multiflora	Rmu_sc0000221.1_g000018 Rmu_sc0000221.1_g000030 Rmu_sc0006732.1_g000016
rosa_roxburghii	Rroxscaffold_3G00257940 Rroxscaffold_3G00257970 Rroxscaffold_3G00257990
rosa_rugosa	Rorug07G0045700 Rorug07G0045700 Rorug07G0045800 Rorug07G0045800 Rorug07G0045900
rosa_samantha	Rh7AG169100 Rh7AG169200 Rh7BG169900 Rh7BG172300 Rh7CG178600 Rh7CG178700 Rh7DG169200 Rh7DG171400 Rh7DG171800
rosa_wichuraiana	Rw7G014570 Rw7G014780 Rw7G014790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1539
Acc16I	TGCGCA	2 cut(s)	1877, 2424
Acc65I	GGTACC	1 cut(s)	2675
AccB1I	GGYRCC	2 cut(s)	2101, 2675
AccB7I	CCANNNNNTGG	1 cut(s)	542
AccI	GTMKAC	1 cut(s)	2701
AccII	CGCG	5 cut(s)	748, 874, 1110, 1598, 3180
AciI	CCGC	9 cut(s)	872, 1007, 1015, 1110, 1127, 1596, 3178, 3596, 3632
AclI	AACGTT	1 cut(s)	2117
AcoI	YGGCCR	3 cut(s)	869, 2430, 2977
AcsI	RAATTY	8 cut(s)	422, 691, 775, 815, 1524, 2837, 2915, 2959
AcuI	CTGAAG	7 cut(s)	246, 1314, 1644, 2582, 2970, 3093, 3114
AcvI	CACGTG	1 cut(s)	219
AcyI	GRCGYC	1 cut(s)	2102
AdeI	CACNNNGTG	1 cut(s)	2479
AfaI	GTAC	9 cut(s)	577, 907, 1216, 1266, 1667, 1987, 2445, 2677, 3356
AfiI	CCNNNNNNNGG	3 cut(s)	542, 1627, 1628
AflIII	ACRYGT	4 cut(s)	216, 218, 858, 974
AhdI	GACNNNNNGTC	1 cut(s)	1791
AjiI	CACGTC	1 cut(s)	2511
AjnI	CCWGG	6 cut(s)	1483, 1741, 2519, 2942, 3518, 3617
AjuI	GAANNNNNNNTTGG	2 cut(s)	3470, 3502
AleI	CACNNNNGTG	1 cut(s)	1823
Alw21I	GWGCWC	4 cut(s)	1276, 1419, 2392, 3579
Alw26I	GTCTC	8 cut(s)	53, 350, 1151, 1496, 1629, 1994, 2102, 3706
AlwNI	CAGNNNCTG	4 cut(s)	1801, 2861, 3092, 3593
Ama87I	CYCGRG	1 cut(s)	2105
AoxI	GGCC	9 cut(s)	532, 869, 948, 1281, 2430, 2644, 2716, 2945, 2977
ApoI	RAATTY	8 cut(s)	422, 691, 775, 815, 1524, 2837, 2915, 2959
ArsI	GACNNNNNNTTYG	2 cut(s)	893, 925
AseI	ATTAAT	1 cut(s)	1164
Asp700I	GAANNNNTTC	1 cut(s)	3527
Asp718I	GGTACC	1 cut(s)	2675
AspS9I	GGNCC	6 cut(s)	533, 661, 755, 1556, 1593, 2716
AsuC2I	CCSGG	4 cut(s)	868, 1023, 2211, 3112
AsuII	TTCGAA	2 cut(s)	1371, 2468
AvaI	CYCGRG	1 cut(s)	2105
AvaII	GGWCC	4 cut(s)	661, 755, 1556, 1593
BaeGI	GKGCMC	1 cut(s)	3254
BaeI	ACNNNNGTAYC	2 cut(s)	2667, 2700
BalI	TGGCCA	1 cut(s)	2432
BamHI	GGATCC	2 cut(s)	1026, 2378
BanI	GGYRCC	2 cut(s)	2101, 2675
BanII	GRGCYC	3 cut(s)	1276, 1419, 3604
BbrPI	CACGTG	1 cut(s)	219
BbsI	GAAGAC	3 cut(s)	542, 2072, 2937
Bbv12I	GWGCWC	4 cut(s)	1276, 1419, 2392, 3579
BccI	CCATC	7 cut(s)	316, 766, 2132, 2225, 2575, 3371, 3619
BceAI	ACGGC	2 cut(s)	1025, 2648
BcgI	CGANNNNNNTGC	4 cut(s)	2387, 2421, 2740, 2774
BciT130I	CCWGG	6 cut(s)	1485, 1743, 2521, 2944, 3520, 3619
BciVI	GTATCC	2 cut(s)	200, 2556
BclI	TGATCA	2 cut(s)	1146, 2706
BcnI	CCSGG	4 cut(s)	868, 1023, 2211, 3112
BcoDI	GTCTC	8 cut(s)	53, 350, 1151, 1496, 1629, 1994, 2102, 3706
BfaI	CTAG	9 cut(s)	98, 399, 822, 1391, 1971, 2004, 2648, 2885, 3083
BfmI	CTRYAG	1 cut(s)	3126
BfoI	RGCGCY	1 cut(s)	2105
BfuI	GTATCC	2 cut(s)	200, 2556
BglII	AGATCT	1 cut(s)	1958
BmcAI	AGTACT	2 cut(s)	577, 1667
Bme18I	GGWCC	4 cut(s)	661, 755, 1556, 1593
BmeRI	GACNNNNNGTC	1 cut(s)	1791
BmeT110I	CYCGRG	1 cut(s)	2105
BmgBI	CACGTC	1 cut(s)	2511
BmgT120I	GGNCC	6 cut(s)	533, 661, 755, 1556, 1593, 2716
BmiI	GGNNCC	8 cut(s)	1028, 1594, 1918, 2103, 2380, 2677, 2717, 3601
BmrI	ACTGGG	2 cut(s)	3256, 3581
BmuI	ACTGGG	2 cut(s)	3256, 3581
BpiI	GAAGAC	3 cut(s)	542, 2072, 2937
BplI	GAGNNNNNCTC	4 cut(s)	1624, 1656, 1991, 2023
BpmI	CTGGAG	2 cut(s)	1806, 2066
Bpu10I	CCTNAGC	2 cut(s)	751, 1285
Bpu14I	TTCGAA	2 cut(s)	1371, 2468
BpuEI	CTTGAG	6 cut(s)	35, 1260, 1493, 2679, 3188, 3755
BpuMI	CCSGG	4 cut(s)	868, 1023, 2211, 3112
Bsa29I	ATCGAT	3 cut(s)	381, 995, 2166
BsaAI	YACGTR	1 cut(s)	219
BsaBI	GATNNNNATC	3 cut(s)	588, 1736, 2201
BsaHI	GRCGYC	1 cut(s)	2102
BsaI	GGTCTC	1 cut(s)	3706
BsaWI	WCCGGW	3 cut(s)	1364, 2678, 3061
BsaXI	ACNNNNNCTCC	2 cut(s)	2319, 2349
Bsc4I	CCNNNNNNNGG	3 cut(s)	542, 1627, 1628
Bse118I	RCCGGY	3 cut(s)	2293, 2975, 3394
Bse1I	ACTGG	5 cut(s)	208, 1823, 2792, 3251, 3587
Bse3DI	GCAATG	7 cut(s)	847, 1617, 2079, 3051, 3264, 3372, 3660
Bse8I	GATNNNNATC	3 cut(s)	588, 1736, 2201
BseBI	CCWGG	6 cut(s)	1485, 1743, 2521, 2944, 3520, 3619
BseCI	ATCGAT	3 cut(s)	381, 995, 2166
BseGI	GGATG	9 cut(s)	181, 1903, 2137, 2143, 2878, 2966, 3382, 3611, 3733
BseJI	GATNNNNATC	3 cut(s)	588, 1736, 2201
BseLI	CCNNNNNNNGG	3 cut(s)	542, 1627, 1628
BseMI	GCAATG	7 cut(s)	847, 1617, 2079, 3051, 3264, 3372, 3660
BseMII	CTCAG	9 cut(s)	510, 765, 930, 1086, 1793, 2114, 2625, 2853, 3315
BseNI	ACTGG	5 cut(s)	208, 1823, 2792, 3251, 3587
BsePI	GCGCGC	1 cut(s)	746
BseRI	GAGGAG	2 cut(s)	1169, 1216
BseSI	GKGCMC	1 cut(s)	3254
BseX3I	CGGCCG	1 cut(s)	869
BseYI	CCCAGC	1 cut(s)	849
BsgI	GTGCAG	2 cut(s)	1847, 3063
Bsh1236I	CGCG	5 cut(s)	748, 874, 1110, 1598, 3180
Bsh1285I	CGRYCG	1 cut(s)	872
BshFI	GGCC	9 cut(s)	534, 871, 950, 1283, 2432, 2646, 2718, 2947, 2979
BshNI	GGYRCC	2 cut(s)	2101, 2675
BshVI	ATCGAT	3 cut(s)	381, 995, 2166
BsiEI	CGRYCG	1 cut(s)	872
BsiHKAI	GWGCWC	4 cut(s)	1276, 1419, 2392, 3579
BsiHKCI	CYCGRG	1 cut(s)	2105
BslFI	GGGAC	4 cut(s)	1126, 1409, 2785, 3017
BslI	CCNNNNNNNGG	3 cut(s)	542, 1627, 1628
BsmAI	GTCTC	8 cut(s)	53, 350, 1151, 1496, 1629, 1994, 2102, 3706
BsmBI	CGTCTC	1 cut(s)	350
BsmFI	GGGAC	4 cut(s)	1126, 1409, 2785, 3017
BsmI	GAATGC	1 cut(s)	3537
BsnI	GGCC	9 cut(s)	534, 871, 950, 1283, 2432, 2646, 2718, 2947, 2979
Bso31I	GGTCTC	1 cut(s)	3706
BsoBI	CYCGRG	1 cut(s)	2105
Bsp119I	TTCGAA	2 cut(s)	1371, 2468
Bsp1286I	GDGCHC	6 cut(s)	1276, 1419, 2392, 3254, 3579, 3604
Bsp1407I	TGTACA	2 cut(s)	1264, 2443
Bsp19I	CCATGG	2 cut(s)	1722, 3635
BspACI	CCGC	9 cut(s)	872, 1007, 1015, 1110, 1127, 1596, 3178, 3596, 3632
BspANI	GGCC	9 cut(s)	534, 871, 950, 1283, 2432, 2646, 2718, 2947, 2979
BspCNI	CTCAG	9 cut(s)	511, 764, 931, 1087, 1794, 2115, 2624, 2854, 3316
BspDI	ATCGAT	3 cut(s)	381, 995, 2166
BspFNI	CGCG	5 cut(s)	748, 874, 1110, 1598, 3180
BspHI	TCATGA	2 cut(s)	646, 2833
BspLI	GGNNCC	8 cut(s)	1028, 1594, 1918, 2103, 2380, 2677, 2717, 3601
BspQI	GCTCTTC	2 cut(s)	365, 3473
BspT104I	TTCGAA	2 cut(s)	1371, 2468
BspT107I	GGYRCC	2 cut(s)	2101, 2675
BspTNI	GGTCTC	1 cut(s)	3706
BsrDI	GCAATG	7 cut(s)	847, 1617, 2079, 3051, 3264, 3372, 3660
BsrFI	RCCGGY	3 cut(s)	2293, 2975, 3394
BsrGI	TGTACA	2 cut(s)	1264, 2443
BsrI	ACTGG	5 cut(s)	208, 1823, 2792, 3251, 3587
BssAI	RCCGGY	3 cut(s)	2293, 2975, 3394
BssHII	GCGCGC	1 cut(s)	746
BssNAI	GTATAC	1 cut(s)	2702
BssNI	GRCGYC	1 cut(s)	2102
BssT1I	CCWWGG	6 cut(s)	1722, 1911, 2889, 2980, 3270, 3635
Bst1107I	GTATAC	1 cut(s)	2702
Bst2UI	CCWGG	6 cut(s)	1485, 1743, 2521, 2944, 3520, 3619
Bst6I	CTCTTC	3 cut(s)	225, 365, 3473
BstACI	GRCGYC	1 cut(s)	2102
BstAPI	GCANNNNNTGC	3 cut(s)	846, 3431, 3659
BstAUI	TGTACA	2 cut(s)	1264, 2443
BstBAI	YACGTR	1 cut(s)	219
BstBI	TTCGAA	2 cut(s)	1371, 2468
BstC8I	GCNNGC	8 cut(s)	568, 748, 985, 2517, 2592, 2629, 3345, 3436
BstDSI	CCRYGG	2 cut(s)	1722, 3635
BstF5I	GGATG	9 cut(s)	181, 1903, 2137, 2143, 2878, 2966, 3382, 3611, 3733
BstFNI	CGCG	5 cut(s)	748, 874, 1110, 1598, 3180
BstH2I	RGCGCY	1 cut(s)	2105
BstMAI	GTCTC	8 cut(s)	53, 350, 1151, 1496, 1629, 1994, 2102, 3706
BstMCI	CGRYCG	1 cut(s)	872
BstNI	CCWGG	6 cut(s)	1485, 1743, 2521, 2944, 3520, 3619
BstNSI	RCATGY	5 cut(s)	862, 929, 978, 987, 2909
BstSFI	CTRYAG	1 cut(s)	3126
BstSLI	GKGCMC	1 cut(s)	3254
BstUI	CGCG	5 cut(s)	748, 874, 1110, 1598, 3180
BstV2I	GAAGAC	3 cut(s)	542, 2072, 2937
BstX2I	RGATCY	4 cut(s)	1026, 1958, 2378, 2607
BstXI	CCANNNNNNTGG	3 cut(s)	2559, 3064, 3724
BstYI	RGATCY	4 cut(s)	1026, 1958, 2378, 2607
BstZ17I	GTATAC	1 cut(s)	2702
BstZI	CGGCCG	1 cut(s)	869
Bsu15I	ATCGAT	3 cut(s)	381, 995, 2166
BsuI	GTATCC	2 cut(s)	200, 2556
BsuRI	GGCC	9 cut(s)	534, 871, 950, 1283, 2432, 2646, 2718, 2947, 2979
BsuTUI	ATCGAT	3 cut(s)	381, 995, 2166
BtgI	CCRYGG	2 cut(s)	1722, 3635
BtrI	CACGTC	1 cut(s)	2511
BtsCI	GGATG	9 cut(s)	181, 1903, 2137, 2143, 2878, 2966, 3382, 3611, 3733
BtsI	GCAGTG	1 cut(s)	2288
BtsIMutI	CAGTG	7 cut(s)	1230, 1830, 2288, 2331, 2799, 2878, 3167
Cac8I	GCNNGC	8 cut(s)	568, 748, 985, 2517, 2592, 2629, 3345, 3436
CaiI	CAGNNNCTG	4 cut(s)	1801, 2861, 3092, 3593
CciI	TCATGA	2 cut(s)	646, 2833
Cfr10I	RCCGGY	3 cut(s)	2293, 2975, 3394
Cfr13I	GGNCC	6 cut(s)	533, 661, 755, 1556, 1593, 2716
ClaI	ATCGAT	3 cut(s)	381, 995, 2166
CseI	GACGC	3 cut(s)	367, 3651, 3664
CsiI	ACCWGGT	1 cut(s)	3617
Csp6I	GTAC	9 cut(s)	576, 906, 1215, 1265, 1666, 1986, 2444, 2676, 3355
CviQI	GTAC	9 cut(s)	576, 906, 1215, 1265, 1666, 1986, 2444, 2676, 3355
DinI	GGCGCC	1 cut(s)	2103
DraIII	CACNNNGTG	1 cut(s)	2479
DriI	GACNNNNNGTC	1 cut(s)	1791
EaeI	YGGCCR	3 cut(s)	869, 2430, 2977
EagI	CGGCCG	1 cut(s)	869
Eam1104I	CTCTTC	3 cut(s)	225, 365, 3473
Eam1105I	GACNNNNNGTC	1 cut(s)	1791
EarI	CTCTTC	3 cut(s)	225, 365, 3473
Ecl136II	GAGCTC	2 cut(s)	1274, 1417
EclXI	CGGCCG	1 cut(s)	869
Eco130I	CCWWGG	6 cut(s)	1722, 1911, 2889, 2980, 3270, 3635
Eco147I	AGGCCT	2 cut(s)	950, 2646
Eco24I	GRGCYC	3 cut(s)	1276, 1419, 3604
Eco31I	GGTCTC	1 cut(s)	3706
Eco47I	GGWCC	4 cut(s)	661, 755, 1556, 1593
Eco52I	CGGCCG	1 cut(s)	869
Eco53kI	GAGCTC	2 cut(s)	1274, 1417
Eco57I	CTGAAG	7 cut(s)	246, 1314, 1644, 2582, 2970, 3093, 3114
Eco72I	CACGTG	1 cut(s)	219
Eco88I	CYCGRG	1 cut(s)	2105
EcoICRI	GAGCTC	2 cut(s)	1274, 1417
EcoRI	GAATTC	1 cut(s)	691
EcoRII	CCWGG	6 cut(s)	1483, 1741, 2519, 2942, 3518, 3617
EcoT14I	CCWWGG	6 cut(s)	1722, 1911, 2889, 2980, 3270, 3635
EcoT22I	ATGCAT	2 cut(s)	589, 891
EcoT38I	GRGCYC	3 cut(s)	1276, 1419, 3604
EgeI	GGCGCC	1 cut(s)	2103
EheI	GGCGCC	1 cut(s)	2103
ErhI	CCWWGG	6 cut(s)	1722, 1911, 2889, 2980, 3270, 3635
Esp3I	CGTCTC	1 cut(s)	350
FalI	AAGNNNNNCTT	4 cut(s)	1365, 1397, 3470, 3502
FaqI	GGGAC	4 cut(s)	1126, 1409, 2785, 3017
FauI	CCCGC	4 cut(s)	1103, 1120, 3185, 3589
FbaI	TGATCA	2 cut(s)	1146, 2706
FblI	GTMKAC	1 cut(s)	2701
FokI	GGATG	9 cut(s)	188, 1910, 2144, 2150, 2865, 2953, 3389, 3598, 3740
FriOI	GRGCYC	3 cut(s)	1276, 1419, 3604
FspBI	CTAG	9 cut(s)	98, 399, 822, 1391, 1971, 2004, 2648, 2885, 3083
FspI	TGCGCA	2 cut(s)	1877, 2424
GsaI	CCCAGC	1 cut(s)	853
GsuI	CTGGAG	2 cut(s)	1806, 2066
HaeII	RGCGCY	1 cut(s)	2105
HaeIII	GGCC	9 cut(s)	534, 871, 950, 1283, 2432, 2646, 2718, 2947, 2979
HgaI	GACGC	3 cut(s)	367, 3651, 3664
Hin1I	GRCGYC	1 cut(s)	2102
HincII	GTYRAC	4 cut(s)	670, 1426, 1785, 2869
HindII	GTYRAC	4 cut(s)	670, 1426, 1785, 2869
HindIII	AAGCTT	2 cut(s)	1084, 2087
HpaI	GTTAAC	1 cut(s)	2869
Hpy188III	TCNNGA	9 cut(s)	647, 753, 1493, 1971, 2045, 2605, 2834, 2911, 3002
Hpy99I	CGWCG	7 cut(s)	63, 886, 913, 1046, 1358, 2512, 3680
HpyCH4IV	ACGT	7 cut(s)	218, 619, 634, 908, 2117, 2510, 3357
HpySE526I	ACGT	7 cut(s)	218, 619, 634, 908, 2117, 2510, 3357
Hsp92I	GRCGYC	1 cut(s)	2102
KasI	GGCGCC	1 cut(s)	2101
KpnI	GGTACC	1 cut(s)	2679
Ksp22I	TGATCA	2 cut(s)	1146, 2706
KspAI	GTTAAC	1 cut(s)	2869
LguI	GCTCTTC	2 cut(s)	365, 3473
LmnI	GCTCC	6 cut(s)	1229, 1810, 2443, 2832, 3599, 3647
MabI	ACCWGGT	1 cut(s)	3617
MaeI	CTAG	9 cut(s)	98, 399, 822, 1391, 1971, 2004, 2648, 2885, 3083
MaeII	ACGT	7 cut(s)	218, 619, 634, 908, 2117, 2510, 3357
MaeIII	GTNAC	6 cut(s)	854, 861, 1090, 3151, 3232, 3239
MfeI	CAATTG	1 cut(s)	3426
MflI	RGATCY	4 cut(s)	1026, 1958, 2378, 2607
MhlI	GDGCHC	6 cut(s)	1276, 1419, 2392, 3254, 3579, 3604
MlsI	TGGCCA	1 cut(s)	2432
MluNI	TGGCCA	1 cut(s)	2432
Mly113I	GGCGCC	1 cut(s)	2102
MlyI	GAGTC	2 cut(s)	2767, 3383
MmeI	TCCRAC	4 cut(s)	41, 1326, 2356, 3021
Mox20I	TGGCCA	1 cut(s)	2432
Mph1103I	ATGCAT	2 cut(s)	589, 891
MroXI	GAANNNNTTC	1 cut(s)	3527
MscI	TGGCCA	1 cut(s)	2432
MslI	CAYNNNNRTG	6 cut(s)	217, 930, 1574, 1823, 2262, 3062
Msp20I	TGGCCA	1 cut(s)	2432
MspA1I	CMGCKG	2 cut(s)	1342, 3461
MunI	CAATTG	1 cut(s)	3426
Mva1269I	GAATGC	1 cut(s)	3537
MvaI	CCWGG	6 cut(s)	1485, 1743, 2521, 2944, 3520, 3619
MvnI	CGCG	5 cut(s)	748, 874, 1110, 1598, 3180
NarI	GGCGCC	1 cut(s)	2102
NciI	CCSGG	4 cut(s)	868, 1023, 2211, 3112
NcoI	CCATGG	2 cut(s)	1722, 3635
NlaIV	GGNNCC	8 cut(s)	1028, 1594, 1918, 2103, 2380, 2677, 2717, 3601
NmuCI	GTSAC	3 cut(s)	854, 861, 3239
NsbI	TGCGCA	2 cut(s)	1877, 2424
NsiI	ATGCAT	2 cut(s)	589, 891
NspI	RCATGY	5 cut(s)	862, 929, 978, 987, 2909
NspV	TTCGAA	2 cut(s)	1371, 2468
OliI	CACNNNNGTG	1 cut(s)	1823
PaeI	GCATGC	1 cut(s)	987
PagI	TCATGA	2 cut(s)	646, 2833
PauI	GCGCGC	1 cut(s)	746
PceI	AGGCCT	2 cut(s)	950, 2646
PciI	ACATGT	2 cut(s)	858, 974
PciSI	GCTCTTC	2 cut(s)	365, 3473
PcsI	WCGNNNNNNNCGW	2 cut(s)	1578, 1704
PctI	GAATGC	1 cut(s)	3537
PdmI	GAANNNNTTC	1 cut(s)	3527
PfeI	GAWTC	9 cut(s)	1238, 1368, 1583, 1798, 2417, 3044, 3115, 3582, 3753
PflFI	GACNNNGTC	1 cut(s)	665
PflMI	CCANNNNNTGG	1 cut(s)	542
PfoI	TCCNGGA	1 cut(s)	3518
PleI	GAGTC	2 cut(s)	2767, 3383
PluTI	GGCGCC	1 cut(s)	2105
PmaCI	CACGTG	1 cut(s)	219
PmlI	CACGTG	1 cut(s)	219
PpsI	GAGTC	2 cut(s)	2767, 3383
Ppu21I	YACGTR	1 cut(s)	219
PscI	ACATGT	2 cut(s)	858, 974
PshBI	ATTAAT	1 cut(s)	1164
PsiI	TTATAA	1 cut(s)	1539
Psp124BI	GAGCTC	2 cut(s)	1276, 1419
Psp1406I	AACGTT	1 cut(s)	2117
Psp6I	CCWGG	6 cut(s)	1483, 1741, 2519, 2942, 3518, 3617
PspCI	CACGTG	1 cut(s)	219
PspFI	CCCAGC	1 cut(s)	849
PspGI	CCWGG	6 cut(s)	1483, 1741, 2519, 2942, 3518, 3617
PspN4I	GGNNCC	8 cut(s)	1028, 1594, 1918, 2103, 2380, 2677, 2717, 3601
PspPI	GGNCC	6 cut(s)	533, 661, 755, 1556, 1593, 2716
PstNI	CAGNNNCTG	4 cut(s)	1801, 2861, 3092, 3593
PsuI	RGATCY	4 cut(s)	1026, 1958, 2378, 2607
PsyI	GACNNNGTC	1 cut(s)	665
PteI	GCGCGC	1 cut(s)	746
PvuII	CAGCTG	2 cut(s)	1342, 3461
RsaI	GTAC	9 cut(s)	577, 907, 1216, 1266, 1667, 1987, 2445, 2677, 3356
RsaNI	GTAC	9 cut(s)	576, 906, 1215, 1265, 1666, 1986, 2444, 2676, 3355
RseI	CAYNNNNRTG	6 cut(s)	217, 930, 1574, 1823, 2262, 3062
SacI	GAGCTC	2 cut(s)	1276, 1419
SapI	GCTCTTC	2 cut(s)	365, 3473
Sau96I	GGNCC	6 cut(s)	533, 661, 755, 1556, 1593, 2716
ScaI	AGTACT	2 cut(s)	577, 1667
SchI	GAGTC	2 cut(s)	2767, 3383
SduI	GDGCHC	6 cut(s)	1276, 1419, 2392, 3254, 3579, 3604
SexAI	ACCWGGT	1 cut(s)	3617
SfcI	CTRYAG	1 cut(s)	3126
SfoI	GGCGCC	1 cut(s)	2103
SfuI	TTCGAA	2 cut(s)	1371, 2468
SinI	GGWCC	4 cut(s)	661, 755, 1556, 1593
SmiMI	CAYNNNNRTG	6 cut(s)	217, 930, 1574, 1823, 2262, 3062
SmlI	CTYRAG	6 cut(s)	50, 1275, 1472, 2694, 3203, 3734
SmoI	CTYRAG	6 cut(s)	50, 1275, 1472, 2694, 3203, 3734
SphI	GCATGC	1 cut(s)	987
SseBI	AGGCCT	2 cut(s)	950, 2646
SsiI	CCGC	9 cut(s)	872, 1007, 1015, 1110, 1127, 1596, 3178, 3596, 3632
SspDI	GGCGCC	1 cut(s)	2101
SspMI	CTAG	9 cut(s)	98, 399, 822, 1391, 1971, 2004, 2648, 2885, 3083
SstI	GAGCTC	2 cut(s)	1276, 1419
StuI	AGGCCT	2 cut(s)	950, 2646
StyI	CCWWGG	6 cut(s)	1722, 1911, 2889, 2980, 3270, 3635
TaiI	ACGT	7 cut(s)	221, 622, 637, 911, 2120, 2513, 3360
TatI	WGTACW	4 cut(s)	575, 1264, 1665, 2443
TauI	GCSGC	2 cut(s)	874, 1018
TfiI	GAWTC	9 cut(s)	1238, 1368, 1583, 1798, 2417, 3044, 3115, 3582, 3753
TscAI	CASTG	7 cut(s)	1237, 1830, 2295, 2338, 2799, 2878, 3174
TseFI	GTSAC	3 cut(s)	854, 861, 3239
Tsp45I	GTSAC	3 cut(s)	854, 861, 3239
TspGWI	ACGGA	2 cut(s)	870, 1019
TspRI	CASTG	7 cut(s)	1237, 1830, 2295, 2338, 2799, 2878, 3174
Tth111I	GACNNNGTC	1 cut(s)	665
Van91I	CCANNNNNTGG	1 cut(s)	542
VpaK11BI	GGWCC	4 cut(s)	661, 755, 1556, 1593
VspI	ATTAAT	1 cut(s)	1164
XapI	RAATTY	8 cut(s)	422, 691, 775, 815, 1524, 2837, 2915, 2959
XbaI	TCTAGA	1 cut(s)	1970
XceI	RCATGY	5 cut(s)	862, 929, 978, 987, 2909
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1256
XmiI	GTMKAC	1 cut(s)	2701
XmnI	GAANNNNTTC	1 cut(s)	3527
XspI	CTAG	9 cut(s)	98, 399, 822, 1391, 1971, 2004, 2648, 2885, 3083
ZrmI	AGTACT	2 cut(s)	577, 1667
Zsp2I	ATGCAT	2 cut(s)	589, 891

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.