Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr7

Orthogroup ID

OG0002025

Physical Location & Seq

Genomic Coordinates Reverse (-)

14259949 .. 14267749

Gene Structure 7801 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw7G014570.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2757 bp

ATGTTCCTCACATATTGCAAAGAAAACAATAATAGAAAGAATTTAATCACCAATTCCAAAGCCGTGTTCTCAGTCATCGGCATGACCTGCTCTGCCTGCACGGGATCAGTAGAGAAGGCTGTGAAGAGGCTACCCGGGATTCCCGAGGCGGTGGTCGACGTGCTCAACAACCATGCCCAAGTCATGTTCTTCCCCAATTTCGTTAACACTATCCGTGAGACAATTGAAGATGTTGGATTTCAAGCAACATTGATTGCTGATGAGGGAAAAGAGAAGCCCACGTTGGTGTGCAGAATAAGGATCAAAGGAATGACTTGCACTTCTTGCTCTTCCACTGTTGAATCAGCTTTGCAGGTAGTTCATGGTGTACAAAAAGCCCAAGTTGCCTTAGCAACTGAAGAAGCAGATGTCCACTATGATCCAAAGATAGTGAGCTGCAATCAGCTGATGGTAGCCATTGAAGACGCTGGATTCGAATCCATACTTATTAATTCAGGTGAAGATCTTAAAGTTGACGGTGTAAGGACCAATCATTCAATGAGAATACTTGAAAAAAATTTCCCTGTCTTACAAGCCAGATATACAGGGCCCCGAAATTTCATCAATGTGATTGAAACAACTGGATCCAGGCGTTTCAGGGCAAAAATTTATCCAGGCGGTGGAGCAGGAAGAGAAAATCATAGGAAGGAGGAAATTCAGCAGTACTACAGAGTCTTTCTCTGGAGTTTGATTTTCACCATTCCAGTATTTTTAACCTCCATGGTCTTCATGTACATCCCTGGACTTAAGCATGGCTTAGACAAGAAAGTAGTAAATATGCTCAGCATTGGGGAGCTTGTAAGGTGGATTCTGTCTACTCCGGTGCAGTTCATCATAGGCTGGAGGTTCTATACTGGGGCATACAAGTCATTGCGCCATGGTTCTGCTAATATGGATGTGTTAATTGCCTTGGGAACAAATGCAGCATATTTCTATTCAGTCTACTCCGTACTGAGAGCTGCCACCTCCCCACATTTTAAGGGTACAGATTTCTTTGAGACTAGTGCAATGCTAATCTCATTCATTCTCTTAGGGAAGTACCTAGAGGTGTTAGCTAAGGGGAAGACATCTGATGCTATTGCCAAACTGATGGATTTGGCACCTGAAACAGCCACTTTGTTGACTCTAGACGAAGAAGGAAACATTTTAGGTGAAGAAGAAATTGATGGTCGGTTGATACAAAAGAACGATGCCATCAAAATTATTCCAGGAGCGAAGGTAGCTTCGGATGGTTATGTGATATGGGGACAAAGCCATGTAAATGAGAGTATGATAACAGGAGAAGCGCGTCCAGTGGCAAAAAGGAAGGGTGATACAGTCATTGGAGGTACTGTAAATGAAAATGGTGTGTTGCATATCAAGGCAACACGGGTTGGATCAGAAAGTTCCCTCGCGCAGATTGTACGGCTTGTTGAGTCAGCTCAGATGGCAAAAGCTCCTGTTCAGAAGTTTGCTGACCGCATTTCTAAATTCTTTGTGCCACTGTTTTTGGCTGGAAAATTCCATGGCTACCCAAAATCTTGGATACCGAAGTCCATGGATAGCTTTCAGCTTGCTCTCCAGTTTGGGATCTCTGTCATGGTTATTGCGTGCCCTTGTGCTCTAGGCCTAGCAACTCCTACTGCTGTCATGGTTGGTACTGGAGTCGGTGCATCCCAAGGTGTACTGATCAAAGGAGGTCAAGCATTAGAAAGTGCACATACGGTGGACTGCATTGTGTTCGACAAGACAGGGACACTTACAATTGGGAAGCCACTGGTTGTTAACACACGACTCCTGAAAAATATGGTGCTTCAAGAATTCTATGAACTTGTTGCTGCAGCAGAGGATGAACACCCCTTGGCCAAGGCCATTGTTGAGTATGCCAAAAAATTCAGAGAAGATGAAGAGAATCCAGCCGGGCCAGAAGCGCATGACTTTGCCTCCATTACTTGTCACAGGGTGAAGGCTATTGTTCGGGGCAGGGAAATAATTGTGGGGAACAAGAGCTTGATGGTGGACCAGAACATTGCAGTTCCACTTGATGCAGAAGACTACCCAGCAGAAGCTGAAGGGCTGGCTCAAACTGGAATTCTAGTAGCCATAGATGGAGAAGTAGCCGGAGTTCTAGCAGTATCTGATCCACTGAAACCAGGTGCTCAAGAAGTAATTACCATACTCAAGTCAATGAATGTTAGAAGCATAATGGTGACAGGTGACAATTGGGGAACTGCAAATTCTATTGCCAATGGAGTTGGTATTGATACTGTTATAGCCGAATCGAAACCTGACCAGAAAGCAGATAAAGTGAAGGAATTGCAGGCTTCAGGCTACACTGTGGCGACGGAGGGATATGGCATCAATGACTCACCAGCACTTGTGGCGGCAGATGTAGGAATGGCAATTGGTGCTGGCACTGACATTGCAATTGAGGCAGCAGACATTGTGCTAATGAAGAGCAACTTGGAGGATGTGATAACTGCCATTGATCTTTCAAGGAAAACCTTCACCCGTATTCGTTTGAACTATATTTGGGCTTTGGGATATAATGTGCTCGGGATCCCAATAGCTGCAGGAGTTCTCTTCCCATCCACTGGATTTCTCTTACCCCCTTGGATTGCTGGAGCTGCAATGGCTGCTTCTTCTGTCAGTGTTGTTTGCTGTTCATTGTTGTTGAAGAACTATAAGAGACCCAAGGAGCTGAATAACCTTGAGGTGCGTGGAATAGTGATCGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0008150 GO:0008324 GO:0009636 GO:0010035 GO:0010038 GO:0010273 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042221 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046688 GO:0046873 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0061687 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0097501 GO:0098655 GO:0098660 GO:0098662 GO:0098754 GO:0099131 GO:0099132 GO:1990169

KEGG Pathways

Metabolic & Signaling

ko01524 ko04016 map01524 map04016

Pfam Domains

Protein Families

E1-E2_ATPase HMA Hydrolase

Protein Analysis

918

Amino Acids

99.36

Weight (kDa)

6.37

Isoelectric Point (pI)

27.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMA	PF00403	22 - 80	8.5e-12	Heavy-metal-associated domain
HMA	PF00403	99 - 159	2.7e-12	Heavy-metal-associated domain
E1-E2_ATPase	PF00122	379 - 485	4.5e-23	P-type ATPase actuator domain
Hydrolase	PF00702	582 - 803	5.9e-26	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0002025)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G63440
fragaria_vesca	FvH4_5g02090 FvH4_5g02090 FvH4_5g02091 FvH4_5g02091 FvH4_5g02091 FvH4_5g02091
malus_domestica	MD06G1080800.v1.1 MD06G1080900.v1.1 MD14G1102600.v1.1
prunus_persica	Prupe.5G097900_v2.0.a1 Prupe.5G097900_v2.0.a1 Prupe.5G098000_v2.0.a1
pyrus_communis	pycom06g07780 pycom06g07790 pycom14g09890
rosa_chinensis	RchiOBHm_Chr7g0197781 RchiOBHm_Chr7g0198111 RchiOBHm_Chr7g0198141
rosa_laevigata	RLG00000003885 RLG00000003888 RLG00000019170
rosa_multiflora	Rmu_sc0000221.1_g000018 Rmu_sc0000221.1_g000030 Rmu_sc0006732.1_g000016
rosa_roxburghii	Rroxscaffold_3G00257940 Rroxscaffold_3G00257970 Rroxscaffold_3G00257990
rosa_rugosa	Rorug07G0045700 Rorug07G0045700 Rorug07G0045800 Rorug07G0045800 Rorug07G0045900
rosa_samantha	Rh7AG169100 Rh7AG169200 Rh7BG169900 Rh7BG172300 Rh7CG178600 Rh7CG178700 Rh7DG169200 Rh7DG171400 Rh7DG171800
rosa_wichuraiana	Rw7G014570 Rw7G014780 Rw7G014790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	95, 343
AccB1I	GGYRCC	1 cut(s)	1138
AccB7I	CCANNNNNTGG	2 cut(s)	659, 2612
AccI	GTMKAC	3 cut(s)	156, 854, 981
AccII	CGCG	2 cut(s)	1327, 1433
AciI	CCGC	4 cut(s)	149, 657, 1498, 2402
AcoI	YGGCCR	1 cut(s)	1881
AcuI	CTGAAG	3 cut(s)	417, 2109, 2328
AdeI	CACNNNGTG	1 cut(s)	1981
AfiI	CCNNNNNNNGG	2 cut(s)	659, 2612
AflII	CTTAAG	1 cut(s)	785
AhlI	ACTAGT	1 cut(s)	1040
AjiI	CACGTC	1 cut(s)	160
AjnI	CCWGG	5 cut(s)	626, 652, 778, 1246, 2170
AjuI	GAANNNNNNNTTGG	6 cut(s)	50, 82, 266, 298, 2465, 2497
AleI	CACNNNNGTG	1 cut(s)	284
Alw21I	GWGCWC	5 cut(s)	165, 1642, 1738, 2179, 2574
Alw26I	GTCTC	3 cut(s)	212, 1031, 2701
Alw44I	GTGCAC	1 cut(s)	1734
AlwNI	CAGNNNCTG	2 cut(s)	2087, 2156
Ama87I	CYCGRG	3 cut(s)	134, 143, 2573
AoxI	GGCC	5 cut(s)	587, 1645, 1881, 1887, 1940
ApaI	GGGCCC	1 cut(s)	591
ApaLI	GTGCAC	1 cut(s)	1734
ApeKI	GCWGC	9 cut(s)	435, 962, 998, 1856, 1859, 2453, 2588, 2645, 2654
ArsI	GACNNNNNNTTYG	2 cut(s)	455, 487
AseI	ATTAAT	1 cut(s)	489
Asp700I	GAANNNNTTC	1 cut(s)	2522
AspLEI	GCGC	4 cut(s)	915, 1327, 1435, 1951
AspS9I	GGNCC	5 cut(s)	525, 587, 588, 1940, 2038
AsuC2I	CCSGG	3 cut(s)	135, 136, 1939
AsuII	TTCGAA	1 cut(s)	474
AvaI	CYCGRG	3 cut(s)	134, 143, 2573
AvaII	GGWCC	2 cut(s)	525, 2038
BaeGI	GKGCMC	3 cut(s)	591, 1634, 1738
BaeI	ACNNNNGTAYC	4 cut(s)	1556, 1589, 1668, 1701
BalI	TGGCCA	1 cut(s)	1883
BamHI	GGATCC	2 cut(s)	623, 2577
BanI	GGYRCC	1 cut(s)	1138
BanII	GRGCYC	1 cut(s)	591
BbsI	GAAGAC	4 cut(s)	468, 757, 1109, 2076
Bbv12I	GWGCWC	5 cut(s)	165, 1642, 1738, 2179, 2574
BbvI	GCAGC	9 cut(s)	422, 974, 985, 1843, 1871, 2465, 2575, 2632, 2641
BccI	CCATC	9 cut(s)	442, 1123, 1199, 1241, 1262, 1459, 2026, 2120, 2614
BceAI	ACGGC	2 cut(s)	47, 1460
BcgI	CGANNNNNNTGC	2 cut(s)	1741, 1775
BciT130I	CCWGG	5 cut(s)	628, 654, 780, 1248, 2172
BciVI	GTATCC	1 cut(s)	1557
BclI	TGATCA	1 cut(s)	1707
BcnI	CCSGG	3 cut(s)	135, 136, 1939
BcoDI	GTCTC	3 cut(s)	212, 1031, 2701
BcuI	ACTAGT	1 cut(s)	1040
BfaI	CTAG	7 cut(s)	1041, 1082, 1166, 1643, 1649, 2114, 2147
BfmI	CTRYAG	3 cut(s)	706, 1857, 2589
BfrI	CTTAAG	1 cut(s)	785
BfuAI	ACCTGC	2 cut(s)	95, 343
BfuI	GTATCC	1 cut(s)	1557
BglII	AGATCT	1 cut(s)	502
BlpI	GCTNAGC	1 cut(s)	821
BmcAI	AGTACT	1 cut(s)	704
Bme1390I	CCNGG	8 cut(s)	135, 136, 628, 654, 780, 1248, 1939, 2172
Bme18I	GGWCC	2 cut(s)	525, 2038
BmeT110I	CYCGRG	3 cut(s)	134, 143, 2573
BmgBI	CACGTC	1 cut(s)	160
BmgT120I	GGNCC	5 cut(s)	525, 587, 588, 1940, 2038
BmiI	GGNNCC	5 cut(s)	589, 590, 625, 1140, 2579
BmrFI	CCNGG	8 cut(s)	135, 136, 628, 654, 780, 1248, 1939, 2172
BmrI	ACTGGG	1 cut(s)	903
BmsI	GCATC	5 cut(s)	1102, 1219, 1700, 2053, 2385
BmuI	ACTGGG	1 cut(s)	903
BpiI	GAAGAC	4 cut(s)	468, 757, 1109, 2076
BplI	GAGNNNNNCTC	2 cut(s)	702, 734
BpmI	CTGGAG	5 cut(s)	742, 901, 1583, 1701, 2661
Bpu10I	CCTNAGC	2 cut(s)	388, 1095
Bpu1102I	GCTNAGC	1 cut(s)	821
Bpu14I	TTCGAA	1 cut(s)	474
BpuEI	CTTGAG	3 cut(s)	2163, 2183, 2750
BpuMI	CCSGG	3 cut(s)	135, 136, 1939
BsaBI	GATNNNNATC	1 cut(s)	475
BsaI	GGTCTC	1 cut(s)	2701
BsaWI	WCCGGW	1 cut(s)	859
BsaXI	ACNNNNNCTCC	6 cut(s)	1242, 1272, 1356, 1386, 1946, 1976
Bsc4I	CCNNNNNNNGG	2 cut(s)	659, 2612
Bse1I	ACTGG	9 cut(s)	625, 743, 898, 1331, 1600, 1684, 1800, 2110, 2617
Bse3DI	GCAATG	5 cut(s)	908, 1053, 2046, 2439, 2655
Bse8I	GATNNNNATC	1 cut(s)	475
BseBI	CCWGG	5 cut(s)	628, 654, 780, 1248, 2172
BseGI	GGATG	7 cut(s)	774, 940, 1273, 1691, 1873, 2494, 2606
BseJI	GATNNNNATC	1 cut(s)	475
BseLI	CCNNNNNNNGG	2 cut(s)	659, 2612
BseMI	GCAATG	5 cut(s)	908, 1053, 2046, 2439, 2655
BseMII	CTCAG	4 cut(s)	84, 835, 983, 1475
BseNI	ACTGG	9 cut(s)	625, 743, 898, 1331, 1600, 1684, 1800, 2110, 2617
BseSI	GKGCMC	3 cut(s)	591, 1634, 1738
BseXI	GCAGC	9 cut(s)	422, 974, 985, 1843, 1871, 2465, 2575, 2632, 2641
BseYI	CCCAGC	1 cut(s)	2077
BsgI	GTGCAG	3 cut(s)	82, 310, 884
Bsh1236I	CGCG	2 cut(s)	1327, 1433
BshFI	GGCC	5 cut(s)	589, 1647, 1883, 1889, 1942
BshNI	GGYRCC	1 cut(s)	1138
BsiHKAI	GWGCWC	5 cut(s)	165, 1642, 1738, 2179, 2574
BsiHKCI	CYCGRG	3 cut(s)	134, 143, 2573
BsiSI	CCGG	4 cut(s)	135, 860, 1938, 2139
BslFI	GGGAC	2 cut(s)	1299, 1786
BslI	CCNNNNNNNGG	2 cut(s)	659, 2612
BsmAI	GTCTC	3 cut(s)	212, 1031, 2701
BsmFI	GGGAC	2 cut(s)	1299, 1786
BsnI	GGCC	5 cut(s)	589, 1647, 1883, 1889, 1942
Bso31I	GGTCTC	1 cut(s)	2701
BsoBI	CYCGRG	3 cut(s)	134, 143, 2573
Bsp119I	TTCGAA	1 cut(s)	474
Bsp120I	GGGCCC	1 cut(s)	587
Bsp1286I	GDGCHC	7 cut(s)	165, 591, 1634, 1642, 1738, 2179, 2574
Bsp1407I	TGTACA	2 cut(s)	367, 771
Bsp1720I	GCTNAGC	1 cut(s)	821
Bsp19I	CCATGG	4 cut(s)	759, 916, 1543, 1575
BspACI	CCGC	4 cut(s)	149, 657, 1498, 2402
BspANI	GGCC	5 cut(s)	589, 1647, 1883, 1889, 1942
BspCNI	CTCAG	4 cut(s)	83, 834, 984, 1474
BspFNI	CGCG	2 cut(s)	1327, 1433
BspLI	GGNNCC	5 cut(s)	589, 590, 625, 1140, 2579
BspMAI	CTGCAG	2 cut(s)	1861, 2593
BspMI	ACCTGC	2 cut(s)	95, 343
BspQI	GCTCTTC	2 cut(s)	334, 2468
BspT104I	TTCGAA	1 cut(s)	474
BspT107I	GGYRCC	1 cut(s)	1138
BspTI	CTTAAG	1 cut(s)	785
BspTNI	GGTCTC	1 cut(s)	2701
BsrDI	GCAATG	5 cut(s)	908, 1053, 2046, 2439, 2655
BsrGI	TGTACA	2 cut(s)	367, 771
BsrI	ACTGG	9 cut(s)	625, 743, 898, 1331, 1600, 1684, 1800, 2110, 2617
Bst2UI	CCWGG	5 cut(s)	628, 654, 780, 1248, 2172
Bst4CI	ACNGT	8 cut(s)	337, 518, 1357, 1372, 1524, 1744, 2287, 2356
Bst6I	CTCTTC	6 cut(s)	119, 334, 664, 1920, 2468, 2606
BstAFI	CTTAAG	1 cut(s)	785
BstAPI	GCANNNNNTGC	4 cut(s)	87, 324, 2426, 2654
BstAUI	TGTACA	2 cut(s)	367, 771
BstBI	TTCGAA	1 cut(s)	474
BstC8I	GCNNGC	6 cut(s)	97, 1593, 1630, 2097, 2340, 2431
BstDEI	CTNAG	8 cut(s)	70, 388, 796, 821, 992, 1069, 1095, 1461
BstDSI	CCRYGG	4 cut(s)	759, 916, 1543, 1575
BstF5I	GGATG	7 cut(s)	774, 940, 1273, 1691, 1873, 2494, 2606
BstFNI	CGCG	2 cut(s)	1327, 1433
BstHHI	GCGC	4 cut(s)	915, 1327, 1435, 1951
BstMAI	GTCTC	3 cut(s)	212, 1031, 2701
BstNI	CCWGG	5 cut(s)	628, 654, 780, 1248, 2172
BstSCI	CCNGG	8 cut(s)	133, 134, 626, 652, 778, 1246, 1937, 2170
BstSFI	CTRYAG	3 cut(s)	706, 1857, 2589
BstSLI	GKGCMC	3 cut(s)	591, 1634, 1738
BstUI	CGCG	2 cut(s)	1327, 1433
BstV1I	GCAGC	9 cut(s)	422, 974, 985, 1843, 1871, 2465, 2575, 2632, 2641
BstV2I	GAAGAC	4 cut(s)	468, 757, 1109, 2076
BstX2I	RGATCY	4 cut(s)	502, 623, 1608, 2577
BstXI	CCANNNNNNTGG	2 cut(s)	1129, 1560
BstYI	RGATCY	4 cut(s)	502, 623, 1608, 2577
BsuI	GTATCC	1 cut(s)	1557
BsuRI	GGCC	5 cut(s)	589, 1647, 1883, 1889, 1942
BtgI	CCRYGG	4 cut(s)	759, 916, 1543, 1575
BtrI	CACGTC	1 cut(s)	160
BtsCI	GGATG	7 cut(s)	774, 940, 1273, 1691, 1873, 2494, 2606
BtsIMutI	CAGTG	9 cut(s)	333, 1338, 1520, 1793, 2162, 2352, 2433, 2610, 2674
BveI	ACCTGC	2 cut(s)	95, 343
Cac8I	GCNNGC	6 cut(s)	97, 1593, 1630, 2097, 2340, 2431
CaiI	CAGNNNCTG	2 cut(s)	2087, 2156
CfoI	GCGC	4 cut(s)	915, 1327, 1435, 1951
Cfr13I	GGNCC	5 cut(s)	525, 587, 588, 1940, 2038
Cfr9I	CCCGGG	1 cut(s)	134
CseI	GACGC	2 cut(s)	473, 1316
CsiI	ACCWGGT	1 cut(s)	2170
CspCI	CAANNNNNGTGG	2 cut(s)	1509, 1544
DdeI	CTNAG	8 cut(s)	70, 388, 796, 821, 992, 1069, 1095, 1461
DraIII	CACNNNGTG	1 cut(s)	1981
EaeI	YGGCCR	1 cut(s)	1881
Eam1104I	CTCTTC	6 cut(s)	119, 334, 664, 1920, 2468, 2606
EarI	CTCTTC	6 cut(s)	119, 334, 664, 1920, 2468, 2606
Eco147I	AGGCCT	1 cut(s)	1647
Eco24I	GRGCYC	1 cut(s)	591
Eco31I	GGTCTC	1 cut(s)	2701
Eco47I	GGWCC	2 cut(s)	525, 2038
Eco57I	CTGAAG	3 cut(s)	417, 2109, 2328
Eco88I	CYCGRG	3 cut(s)	134, 143, 2573
EcoO109I	RGGNCCY	2 cut(s)	587, 588
EcoRI	GAATTC	2 cut(s)	1838, 2109
EcoRII	CCWGG	5 cut(s)	626, 652, 778, 1246, 2170
EcoT38I	GRGCYC	1 cut(s)	591
FalI	AAGNNNNNCTT	4 cut(s)	779, 811, 2465, 2497
FaqI	GGGAC	2 cut(s)	1299, 1786
FbaI	TGATCA	1 cut(s)	1707
FblI	GTMKAC	3 cut(s)	156, 854, 981
FokI	GGATG	7 cut(s)	761, 947, 1280, 1678, 1880, 2501, 2593
FriOI	GRGCYC	1 cut(s)	591
FspBI	CTAG	7 cut(s)	1041, 1082, 1166, 1643, 1649, 2114, 2147
GlaI	GCGC	4 cut(s)	914, 1326, 1434, 1950
GsaI	CCCAGC	1 cut(s)	2081
GsuI	CTGGAG	5 cut(s)	742, 901, 1583, 1701, 2661
HaeIII	GGCC	5 cut(s)	589, 1647, 1883, 1889, 1942
HapII	CCGG	4 cut(s)	135, 860, 1938, 2139
HgaI	GACGC	2 cut(s)	473, 1316
HhaI	GCGC	4 cut(s)	915, 1327, 1435, 1951
Hin6I	GCGC	4 cut(s)	913, 1325, 1433, 1949
HinP1I	GCGC	4 cut(s)	913, 1325, 1433, 1949
HincII	GTYRAC	5 cut(s)	157, 205, 514, 1161, 1804
HindII	GTYRAC	5 cut(s)	157, 205, 514, 1161, 1804
HpaI	GTTAAC	2 cut(s)	205, 1804
HpaII	CCGG	4 cut(s)	135, 860, 1938, 2139
Hpy188I	TCNGA	7 cut(s)	1111, 1267, 1420, 1464, 1485, 1916, 2158
Hpy188III	TCNNGA	7 cut(s)	143, 721, 1166, 1816, 1835, 2180, 2575
Hpy99I	CGWCG	2 cut(s)	161, 2365
HpyAV	CCTTC	9 cut(s)	109, 679, 1169, 1249, 1339, 1978, 2084, 2323, 2533
HpyCH4III	ACNGT	8 cut(s)	337, 518, 1357, 1372, 1524, 1744, 2287, 2356
HpyCH4IV	ACGT	2 cut(s)	159, 281
HpyF3I	CTNAG	8 cut(s)	70, 388, 796, 821, 992, 1069, 1095, 1461
HpySE526I	ACGT	2 cut(s)	159, 281
HspAI	GCGC	4 cut(s)	913, 1325, 1433, 1949
Ksp22I	TGATCA	1 cut(s)	1707
KspAI	GTTAAC	2 cut(s)	205, 1804
LguI	GCTCTTC	2 cut(s)	334, 2468
LmnI	GCTCC	6 cut(s)	662, 832, 1250, 1480, 2642, 2716
Lsp1109I	GCAGC	9 cut(s)	422, 974, 985, 1843, 1871, 2465, 2575, 2632, 2641
LweI	GCATC	5 cut(s)	1102, 1219, 1700, 2053, 2385
MabI	ACCWGGT	1 cut(s)	2170
MaeI	CTAG	7 cut(s)	1041, 1082, 1166, 1643, 1649, 2114, 2147
MaeII	ACGT	2 cut(s)	159, 281
MaeIII	GTNAC	3 cut(s)	1973, 2227, 2234
MfeI	CAATTG	5 cut(s)	222, 1782, 2239, 2421, 2445
MflI	RGATCY	4 cut(s)	502, 623, 1608, 2577
MhlI	GDGCHC	7 cut(s)	165, 591, 1634, 1642, 1738, 2179, 2574
MlsI	TGGCCA	1 cut(s)	1883
MluNI	TGGCCA	1 cut(s)	1883
MlyI	GAGTC	6 cut(s)	720, 1156, 1463, 1692, 1806, 2378
MmeI	TCCRAC	2 cut(s)	214, 1393
Mox20I	TGGCCA	1 cut(s)	1883
MroXI	GAANNNNTTC	1 cut(s)	2522
MscI	TGGCCA	1 cut(s)	1883
MseI	TTAA	9 cut(s)	44, 204, 489, 507, 752, 786, 941, 1017, 1803
MslI	CAYNNNNRTG	5 cut(s)	80, 284, 605, 1299, 2225
Msp20I	TGGCCA	1 cut(s)	1883
MspA1I	CMGCKG	1 cut(s)	445
MspCI	CTTAAG	1 cut(s)	785
MspI	CCGG	4 cut(s)	135, 860, 1938, 2139
MspR9I	CCNGG	8 cut(s)	135, 136, 628, 654, 780, 1248, 1939, 2172
MunI	CAATTG	5 cut(s)	222, 1782, 2239, 2421, 2445
MvaI	CCWGG	5 cut(s)	628, 654, 780, 1248, 2172
MvnI	CGCG	2 cut(s)	1327, 1433
NciI	CCSGG	3 cut(s)	135, 136, 1939
NcoI	CCATGG	4 cut(s)	759, 916, 1543, 1575
NlaIV	GGNNCC	5 cut(s)	589, 590, 625, 1140, 2579
NmuCI	GTSAC	3 cut(s)	1973, 2227, 2234
NspV	TTCGAA	1 cut(s)	474
OliI	CACNNNNGTG	1 cut(s)	284
PceI	AGGCCT	1 cut(s)	1647
PciSI	GCTCTTC	2 cut(s)	334, 2468
PcsI	WCGNNNNNNNCGW	1 cut(s)	471
PdmI	GAANNNNTTC	1 cut(s)	2522
PfeI	GAWTC	7 cut(s)	139, 341, 471, 476, 847, 1930, 2297
PflMI	CCANNNNNTGG	2 cut(s)	659, 2612
PfoI	TCCNGGA	1 cut(s)	1246
PleI	GAGTC	6 cut(s)	719, 1156, 1462, 1691, 1806, 2378
PpsI	GAGTC	6 cut(s)	719, 1156, 1462, 1691, 1806, 2378
PshBI	ATTAAT	1 cut(s)	489
Psp6I	CCWGG	5 cut(s)	626, 652, 778, 1246, 2170
PspFI	CCCAGC	1 cut(s)	2077
PspGI	CCWGG	5 cut(s)	626, 652, 778, 1246, 2170
PspN4I	GGNNCC	5 cut(s)	589, 590, 625, 1140, 2579
PspOMI	GGGCCC	1 cut(s)	587
PspPI	GGNCC	5 cut(s)	525, 587, 588, 1940, 2038
PsrI	GAACNNNNNNTAC	2 cut(s)	2127, 2159
PstI	CTGCAG	2 cut(s)	1861, 2593
PstNI	CAGNNNCTG	2 cut(s)	2087, 2156
PsuI	RGATCY	4 cut(s)	502, 623, 1608, 2577
PvuII	CAGCTG	1 cut(s)	445
RseI	CAYNNNNRTG	5 cut(s)	80, 284, 605, 1299, 2225
SalI	GTCGAC	1 cut(s)	155
SapI	GCTCTTC	2 cut(s)	334, 2468
SaqAI	TTAA	9 cut(s)	44, 204, 489, 507, 752, 786, 941, 1017, 1803
Sau96I	GGNCC	5 cut(s)	525, 587, 588, 1940, 2038
ScaI	AGTACT	1 cut(s)	704
SchI	GAGTC	6 cut(s)	720, 1156, 1463, 1692, 1806, 2378
ScrFI	CCNGG	8 cut(s)	135, 136, 628, 654, 780, 1248, 1939, 2172
SduI	GDGCHC	7 cut(s)	165, 591, 1634, 1642, 1738, 2179, 2574
SexAI	ACCWGGT	1 cut(s)	2170
SfaNI	GCATC	5 cut(s)	1102, 1219, 1700, 2053, 2385
SfcI	CTRYAG	3 cut(s)	706, 1857, 2589
SfuI	TTCGAA	1 cut(s)	474
SinI	GGWCC	2 cut(s)	525, 2038
SmaI	CCCGGG	1 cut(s)	136
SmiMI	CAYNNNNRTG	5 cut(s)	80, 284, 605, 1299, 2225
SmlI	CTYRAG	4 cut(s)	785, 2178, 2198, 2729
SmoI	CTYRAG	4 cut(s)	785, 2178, 2198, 2729
SpeI	ACTAGT	1 cut(s)	1040
SseBI	AGGCCT	1 cut(s)	1647
SsiI	CCGC	4 cut(s)	149, 657, 1498, 2402
SspMI	CTAG	7 cut(s)	1041, 1082, 1166, 1643, 1649, 2114, 2147
StuI	AGGCCT	1 cut(s)	1647
StyD4I	CCNGG	8 cut(s)	133, 134, 626, 652, 778, 1246, 1937, 2170
TaaI	ACNGT	8 cut(s)	337, 518, 1357, 1372, 1524, 1744, 2287, 2356
TaiI	ACGT	2 cut(s)	162, 284
TaqI	TCGA	5 cut(s)	156, 474, 1761, 2300, 2751
TatI	WGTACW	4 cut(s)	367, 702, 771, 1702
TauI	GCSGC	1 cut(s)	2405
TfiI	GAWTC	7 cut(s)	139, 341, 471, 476, 847, 1930, 2297
Tru1I	TTAA	9 cut(s)	44, 204, 489, 507, 752, 786, 941, 1017, 1803
Tru9I	TTAA	9 cut(s)	44, 204, 489, 507, 752, 786, 941, 1017, 1803
TscAI	CASTG	9 cut(s)	340, 1338, 1527, 1800, 2169, 2359, 2440, 2617, 2674
TseFI	GTSAC	3 cut(s)	1973, 2227, 2234
TseI	GCWGC	9 cut(s)	435, 962, 998, 1856, 1859, 2453, 2588, 2645, 2654
Tsp45I	GTSAC	3 cut(s)	1973, 2227, 2234
TspGWI	ACGGA	3 cut(s)	203, 976, 2378
TspMI	CCCGGG	1 cut(s)	134
TspRI	CASTG	9 cut(s)	340, 1338, 1527, 1800, 2169, 2359, 2440, 2617, 2674
Van91I	CCANNNNNTGG	2 cut(s)	659, 2612
Vha464I	CTTAAG	1 cut(s)	785
VneI	GTGCAC	1 cut(s)	1734
VpaK11BI	GGWCC	2 cut(s)	525, 2038
VspI	ATTAAT	1 cut(s)	489
XbaI	TCTAGA	1 cut(s)	1165
XmaI	CCCGGG	1 cut(s)	134
XmiI	GTMKAC	3 cut(s)	156, 854, 981
XmnI	GAANNNNTTC	1 cut(s)	2522
XspI	CTAG	7 cut(s)	1041, 1082, 1166, 1643, 1649, 2114, 2147
ZrmI	AGTACT	1 cut(s)	704

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.