Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0002025

Physical Location & Seq

Genomic Coordinates Forward (+)

52303516 .. 52309312

Gene Structure 5797 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00257970.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2769 bp

ATGGCGACCAAGTTCTTTGCTCTTGCGTGCATACGCAACAACAACAGTGGTGAAGGCCGCGGCGGCAGCGATTTGGCCCCGCGGCCTCACTACCCCTCCATGCCAAAGTATCCGAAGGGTGTGGCGGCGGAGGAGACCACTATGGCGGTGGGCACCGAGTCCAAGGCCGTGTTCTCCGTCATCGGCATGACCTGCTCCGCCTGCGCGGGATCGGTAGAGAAGGCTGTGAAGAGGCTACCGGGGATTCGCGAGGCGGTGGTCGACGTGCTCAACAACCGTGCCCAAGTCATGTTCTTCCCCAAGTTCGTTAACGCAGAGACTATCCGTGAGACTATTGAAGATGTTGGATTTCAAGCAACATTGATTGCTGATGAGGGAAACGAGAAGTCCACGTTGGTGTGCAGAATAAGGATCAAAGGAATGACTTGCACTTCTTGCTCTTCCACTGTTGAATCAGCTTTGCAGGCAGTTCATGGTGTGCAAAGAGCCCAAGTTGCCTTAGCAACTGAAGAAGCAGATGTCCACTATGATCCAAAGATAGTGAGCTGCAATCAGCTGATGGTAGCCATTGAAGACACTGGATTTGAAGCCGTACTTATTAATTCAGGTGAAGACATGAGCAAGATAGATCTTAAAGTTGACGGTGTAAGGACTGATCATTCAATGAGAATACTTGAACAGTCTCTTCAAGCACTCCCTGGGGTGCAAGGCGGTGGAGCAGGAAGAGAAAATCATAGGAAGGAGGAAATTCAGCAGTACTACAGAGTCTTTCTCTGGAGTTTGATTTTCACCATTCCAGTATTTTTAACCTCCATGGTCCTCATGTACATCCCTGGACTTAAGCATGGCTTAGACAAGAAAGTAGTAAATATGCTCAGCATTGGGGAGCTTATAAGGTGGATTCTGTCTACGCCGGTGCAGTTCATCATAGGCCGGAGGTTCTATACTGGGGCATACAAGTCATTGCGCCATGGTTCTGCTAATATGGATGTGTTAATTGCCTTGGGAACAAATGCAGCATATTTCTATTCAGTCTACTCCGGGAAATACCTAGAGGTGTTAGCTAAGGGGAAGACATCTGATGCTATTGCCAAACTGATGGATTTGGCACCTGAAACAGCCACTTTGTTGACTCTAGACGAAGAAGGAAACGTGTTAGGTGAAGAAGAAATTGATGGTCGGTTGATACAAAAGAACGATGTCATCAAAATTATTCCAGGGGCGAAAGTAGCTTCTGATGGTTATGTGATATGGGGACAAAGCCATGTAAATGAGAGTATGATAACAGGAGAAGCGCGTCCAGTGGCAAAAAGGAAGGGTGATACAGTCATTGGAGGTACTGTAAATGAAAATGGTGTGTTGCATATCAAGGCAACACGGGTTGGATCAGAAAGTTCCCTTGCGCAGATTGTACGGCTTGTTGAGTCAGCTCAGATGGCAAAAGCTCCTGTTCAGAAGTTTGCTGACCGCATTTCTAAATTCTTTGTGCCACTGGTGATCATGCTTTCATTCTTTACCTGGCTTTCATGGTTTTTGGCTGGAAAATTCCATGGCTACCCGAAATCTTGGATACCGAAGTCCATGGATAGCTTTCAGCTTGCTCTCCAGTTTGGGATCTCTGTCATGGTTATTGCGTGCCCTTGTGCTCTAGGCCTAGCAACTCCTACTGCTGTCATGGTCGGTACTGGAGTTGGTGCATCCCAAGGTGTACTGATCAAAGGAGGTCAAGCATTAGAAAGTGCACATAAGGTGGACTGCATTGTGTTCGACAAGACAGGGACTCTTACAATTGGGAAGCCACTGGTTGTTAACACACGACTCCTGAAAAATATGGTGCTTCGAGAATTCTATGAACTTGTTGCTGCAGCTGAGGTTAACAGTGAACACCCCTTGGCCAAGGCCATTGTTGAGTATGCCAAAAAATTCAGAGAAGATGAAGAGAATCCAACCGGGCCAGAAGCGCATGACTTTGCCTCCATTACTGGTCACGGGGTGAAGGCTATTGTTCGGGGCAGGGAAATAATTGTGGGGAACAAGAGCTTGATGGTGGACCAGAACATTGCAGTTCCACTCGATGCAGAAGACTACCTAGCAGAAGCTGAAGGGCTAGCTCAAACTGGGATTCTAGTAGCCATAGATGGAGAAGTGGCCGGAGTTCTAGCAATATCTGATCCACTGAAACCAGGTGCTCAAGAAGTAATTACCATACTCAAGTCAATGAATGTTACAAGCATAATGGTGACAGGTGACAATTGGGGAACTGCAAATTCTATTGCCAATGAAGTTGGTATTGATACTGTTATAGCCGAAGCGAAACCTGACCAGAAAGCAGAGAAAGTGAAGGAATTGCAGGCTTCAGGCTACACTGTGGCGATGGTGGGAGATGGCATCAATGACTCACCAGCACTTGTGGCAGCAGATGTAGGAATGGCAATTGGTGCCGGCACTGACATTGCAATTGAGGCAGCAGACATTGTGCTAATGAAGAGCAACTTGGAGGATGTGATAACTGCCATTGATCTTTCAAGGAAAACTTTCACCCGTATTCGTTTGAACTACATTTGGGCTTTGGGATATAATGTGCTCGGGATCCCAATAGCTGCAGGAGTTCTCTTCCCATCCACTGGATTTCGCTTACCCCCTTGGATTGCTGGAGCTGCAATGGCTGCTTCTTCTGTCAGTGTTGTTTGCTGTTCATTGTTGTTGAAGAACTATAAGAGACCCAAGGAGCTGAATAACCTTGAGGTGCGTGGAATAGTGATCGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0008150 GO:0008324 GO:0009636 GO:0010035 GO:0010038 GO:0010273 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042221 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046688 GO:0046873 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0061687 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0097501 GO:0098655 GO:0098660 GO:0098662 GO:0098754 GO:0099131 GO:0099132 GO:1990169

KEGG Pathways

Metabolic & Signaling

ko01524 ko04016 map01524 map04016

Pfam Domains

Protein Families

E1-E2_ATPase HMA Hydrolase

Protein Analysis

922

Amino Acids

99.16

Weight (kDa)

5.92

Isoelectric Point (pI)

27.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMA	PF00403	57 - 117	3.5e-14	Heavy-metal-associated domain
HMA	PF00403	136 - 196	1.6e-12	Heavy-metal-associated domain
E1-E2_ATPase	PF00122	369 - 475	2e-23	P-type ATPase actuator domain
Hydrolase	PF00702	584 - 807	2.2e-34	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0002025)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G63440
fragaria_vesca	FvH4_5g02090 FvH4_5g02090 FvH4_5g02091 FvH4_5g02091 FvH4_5g02091 FvH4_5g02091
malus_domestica	MD06G1080800.v1.1 MD06G1080900.v1.1 MD14G1102600.v1.1
prunus_persica	Prupe.5G097900_v2.0.a1 Prupe.5G097900_v2.0.a1 Prupe.5G098000_v2.0.a1
pyrus_communis	pycom06g07780 pycom06g07790 pycom14g09890
rosa_chinensis	RchiOBHm_Chr7g0197781 RchiOBHm_Chr7g0198111 RchiOBHm_Chr7g0198141
rosa_laevigata	RLG00000003885 RLG00000003888 RLG00000019170
rosa_multiflora	Rmu_sc0000221.1_g000018 Rmu_sc0000221.1_g000030 Rmu_sc0006732.1_g000016
rosa_roxburghii	Rroxscaffold_3G00257940 Rroxscaffold_3G00257970 Rroxscaffold_3G00257990
rosa_rugosa	Rorug07G0045700 Rorug07G0045700 Rorug07G0045800 Rorug07G0045800 Rorug07G0045900
rosa_samantha	Rh7AG169100 Rh7AG169200 Rh7BG169900 Rh7BG172300 Rh7CG178600 Rh7CG178700 Rh7DG169200 Rh7DG171400 Rh7DG171800
rosa_wichuraiana	Rw7G014570 Rw7G014780 Rw7G014790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	893
Acc16I	TGCGCA	1 cut(s)	1404
Acc36I	ACCTGC	1 cut(s)	200
AccB1I	GGYRCC	3 cut(s)	152, 1108, 2438
AccB7I	CCANNNNNTGG	1 cut(s)	2624
AccI	GTMKAC	3 cut(s)	261, 908, 1035
AccII	CGCG	5 cut(s)	60, 82, 206, 249, 1297
AclWI	GGATC	8 cut(s)	217, 419, 524, 1393, 1622, 2165, 2584, 2597
AcoI	YGGCCR	2 cut(s)	1893, 2148
AcsI	RAATTY	6 cut(s)	747, 1478, 1544, 1844, 1922, 2266
AcuI	CTGAAG	3 cut(s)	528, 2121, 2340
AdeI	CACNNNGTG	1 cut(s)	1993
AfaI	GTAC	7 cut(s)	594, 758, 827, 1339, 1413, 1684, 1710
AfiI	CCNNNNNNNGG	1 cut(s)	2624
AflII	CTTAAG	1 cut(s)	839
AflIII	ACRYGT	1 cut(s)	1152
AjiI	CACGTC	1 cut(s)	265
AjnI	CCWGG	5 cut(s)	697, 832, 1216, 1517, 2182
AjuI	GAANNNNNNNTTGG	6 cut(s)	155, 187, 377, 409, 2477, 2509
AleI	CACNNNNGTG	1 cut(s)	395
Alw21I	GWGCWC	5 cut(s)	270, 1648, 1744, 2191, 2586
Alw26I	GTCTC	5 cut(s)	128, 311, 323, 687, 2713
Alw44I	GTGCAC	1 cut(s)	1740
AlwI	GGATC	8 cut(s)	217, 419, 524, 1393, 1622, 2165, 2584, 2597
AlwNI	CAGNNNCTG	1 cut(s)	2099
Ama87I	CYCGRG	1 cut(s)	2585
ApaLI	GTGCAC	1 cut(s)	1740
ApoI	RAATTY	6 cut(s)	747, 1478, 1544, 1844, 1922, 2266
ArsI	GACNNNNNNTTYG	2 cut(s)	566, 598
AseI	ATTAAT	1 cut(s)	600
Asp700I	GAANNNNTTC	1 cut(s)	2534
AspLEI	GCGC	5 cut(s)	206, 969, 1297, 1405, 1963
AspS9I	GGNCC	4 cut(s)	76, 817, 1952, 2050
AsuC2I	CCSGG	3 cut(s)	240, 1042, 1951
AsuNHI	GCTAGC	1 cut(s)	2107
AvaI	CYCGRG	1 cut(s)	2585
AvaII	GGWCC	2 cut(s)	817, 2050
BaeGI	GKGCMC	4 cut(s)	155, 283, 1640, 1744
BaeI	ACNNNNGTAYC	4 cut(s)	1562, 1595, 1674, 1707
BalI	TGGCCA	1 cut(s)	1895
BamHI	GGATCC	1 cut(s)	2589
BanI	GGYRCC	3 cut(s)	152, 1108, 2438
BanII	GRGCYC	1 cut(s)	490
BbsI	GAAGAC	4 cut(s)	579, 618, 1079, 2088
Bbv12I	GWGCWC	5 cut(s)	270, 1648, 1744, 2191, 2586
BbvCI	CCTCAGC	1 cut(s)	1869
BceAI	ACGGC	3 cut(s)	152, 575, 1430
BcgI	CGANNNNNNTGC	2 cut(s)	1747, 1781
BciT130I	CCWGG	5 cut(s)	699, 834, 1218, 1519, 2184
BciVI	GTATCC	2 cut(s)	120, 1563
BclI	TGATCA	3 cut(s)	655, 1497, 1713
BcnI	CCSGG	3 cut(s)	240, 1042, 1951
BcoDI	GTCTC	5 cut(s)	128, 311, 323, 687, 2713
BfaI	CTAG	8 cut(s)	1052, 1136, 1649, 1655, 2090, 2108, 2126, 2159
BfmI	CTRYAG	3 cut(s)	760, 1863, 2601
BfrI	CTTAAG	1 cut(s)	839
BfuAI	ACCTGC	1 cut(s)	200
BfuI	GTATCC	2 cut(s)	120, 1563
BglI	GCCNNNNNGGC	1 cut(s)	63
BglII	AGATCT	1 cut(s)	628
BlpI	GCTNAGC	1 cut(s)	875
BmcAI	AGTACT	1 cut(s)	758
Bme1390I	CCNGG	8 cut(s)	240, 699, 834, 1042, 1218, 1519, 1951, 2184
Bme18I	GGWCC	2 cut(s)	817, 2050
BmeT110I	CYCGRG	1 cut(s)	2585
BmgBI	CACGTC	1 cut(s)	265
BmgT120I	GGNCC	4 cut(s)	76, 817, 1952, 2050
BmiI	GGNNCC	5 cut(s)	78, 154, 1110, 2440, 2591
BmrFI	CCNGG	8 cut(s)	240, 699, 834, 1042, 1218, 1519, 1951, 2184
BmrI	ACTGGG	2 cut(s)	957, 2127
BmsI	GCATC	4 cut(s)	1072, 1706, 2065, 2397
BmtI	GCTAGC	1 cut(s)	2111
BmuI	ACTGGG	2 cut(s)	957, 2127
BpiI	GAAGAC	4 cut(s)	579, 618, 1079, 2088
BplI	GAGNNNNNCTC	2 cut(s)	756, 788
BpmI	CTGGAG	4 cut(s)	796, 1589, 1707, 2673
Bpu10I	CCTNAGC	3 cut(s)	499, 1065, 1869
Bpu1102I	GCTNAGC	1 cut(s)	875
BpuEI	CTTGAG	3 cut(s)	2175, 2195, 2762
BpuMI	CCSGG	3 cut(s)	240, 1042, 1951
BsaI	GGTCTC	2 cut(s)	128, 2713
BsaXI	ACNNNNNCTCC	6 cut(s)	80, 110, 1326, 1356, 1958, 1988
Bsc4I	CCNNNNNNNGG	1 cut(s)	2624
Bse118I	RCCGGY	2 cut(s)	913, 2441
Bse3DI	GCAATG	4 cut(s)	962, 2058, 2451, 2667
BseBI	CCWGG	5 cut(s)	699, 834, 1218, 1519, 2184
BseGI	GGATG	5 cut(s)	828, 994, 1697, 2506, 2618
BseLI	CCNNNNNNNGG	1 cut(s)	2624
BseMI	GCAATG	4 cut(s)	962, 2058, 2451, 2667
BseMII	CTCAG	3 cut(s)	889, 1445, 1860
BseRI	GAGGAG	1 cut(s)	146
BseSI	GKGCMC	4 cut(s)	155, 283, 1640, 1744
BsgI	GTGCAG	2 cut(s)	421, 938
Bsh1236I	CGCG	5 cut(s)	60, 82, 206, 249, 1297
BshNI	GGYRCC	3 cut(s)	152, 1108, 2438
BsiHKAI	GWGCWC	5 cut(s)	270, 1648, 1744, 2191, 2586
BsiHKCI	CYCGRG	1 cut(s)	2585
BsiSI	CCGG	7 cut(s)	239, 914, 934, 1041, 1950, 2151, 2442
BslFI	GGGAC	2 cut(s)	1269, 1792
BslI	CCNNNNNNNGG	1 cut(s)	2624
BsmAI	GTCTC	5 cut(s)	128, 311, 323, 687, 2713
BsmFI	GGGAC	2 cut(s)	1269, 1792
Bso31I	GGTCTC	2 cut(s)	128, 2713
BsoBI	CYCGRG	1 cut(s)	2585
Bsp1286I	GDGCHC	9 cut(s)	155, 270, 283, 490, 1640, 1648, 1744, 2191, 2586
Bsp1407I	TGTACA	1 cut(s)	825
Bsp1720I	GCTNAGC	1 cut(s)	875
Bsp19I	CCATGG	4 cut(s)	813, 970, 1549, 1581
Bsp68I	TCGCGA	1 cut(s)	249
BspCNI	CTCAG	3 cut(s)	888, 1444, 1861
BspFNI	CGCG	5 cut(s)	60, 82, 206, 249, 1297
BspLI	GGNNCC	5 cut(s)	78, 154, 1110, 2440, 2591
BspMAI	CTGCAG	2 cut(s)	1867, 2605
BspMI	ACCTGC	1 cut(s)	200
BspOI	GCTAGC	1 cut(s)	2111
BspPI	GGATC	8 cut(s)	217, 419, 524, 1393, 1622, 2165, 2584, 2597
BspQI	GCTCTTC	2 cut(s)	445, 2480
BspT107I	GGYRCC	3 cut(s)	152, 1108, 2438
BspTI	CTTAAG	1 cut(s)	839
BspTNI	GGTCTC	2 cut(s)	128, 2713
BsrDI	GCAATG	4 cut(s)	962, 2058, 2451, 2667
BsrFI	RCCGGY	2 cut(s)	913, 2441
BsrGI	TGTACA	1 cut(s)	825
BssAI	RCCGGY	2 cut(s)	913, 2441
Bst2UI	CCWGG	5 cut(s)	699, 834, 1218, 1519, 2184
Bst6I	CTCTTC	7 cut(s)	224, 445, 690, 718, 1932, 2480, 2618
BstAFI	CTTAAG	1 cut(s)	839
BstAPI	GCANNNNNTGC	4 cut(s)	192, 435, 2438, 2666
BstAUI	TGTACA	1 cut(s)	825
BstC8I	GCNNGC	8 cut(s)	28, 202, 465, 1599, 1636, 2109, 2352, 2443
BstDEI	CTNAG	6 cut(s)	499, 850, 875, 1065, 1431, 1869
BstDSI	CCRYGG	6 cut(s)	58, 80, 813, 970, 1549, 1581
BstF5I	GGATG	5 cut(s)	828, 994, 1697, 2506, 2618
BstFNI	CGCG	5 cut(s)	60, 82, 206, 249, 1297
BstHHI	GCGC	5 cut(s)	206, 969, 1297, 1405, 1963
BstMAI	GTCTC	5 cut(s)	128, 311, 323, 687, 2713
BstNI	CCWGG	5 cut(s)	699, 834, 1218, 1519, 2184
BstSCI	CCNGG	8 cut(s)	238, 697, 832, 1040, 1216, 1517, 1949, 2182
BstSFI	CTRYAG	3 cut(s)	760, 1863, 2601
BstSLI	GKGCMC	4 cut(s)	155, 283, 1640, 1744
BstUI	CGCG	5 cut(s)	60, 82, 206, 249, 1297
BstV2I	GAAGAC	4 cut(s)	579, 618, 1079, 2088
BstX2I	RGATCY	3 cut(s)	628, 1614, 2589
BstXI	CCANNNNNNTGG	1 cut(s)	1099
BstYI	RGATCY	3 cut(s)	628, 1614, 2589
BsuI	GTATCC	2 cut(s)	120, 1563
BtgI	CCRYGG	6 cut(s)	58, 80, 813, 970, 1549, 1581
BtgZI	GCGATG	1 cut(s)	2387
BtrI	CACGTC	1 cut(s)	265
BtsCI	GGATG	5 cut(s)	828, 994, 1697, 2506, 2618
BtuMI	TCGCGA	1 cut(s)	249
BveI	ACCTGC	1 cut(s)	200
Cac8I	GCNNGC	8 cut(s)	28, 202, 465, 1599, 1636, 2109, 2352, 2443
CaiI	CAGNNNCTG	1 cut(s)	2099
CfoI	GCGC	5 cut(s)	206, 969, 1297, 1405, 1963
Cfr10I	RCCGGY	2 cut(s)	913, 2441
Cfr13I	GGNCC	4 cut(s)	76, 817, 1952, 2050
Cfr42I	CCGCGG	2 cut(s)	61, 83
CseI	GACGC	1 cut(s)	1286
CsiI	ACCWGGT	1 cut(s)	2182
Csp6I	GTAC	7 cut(s)	593, 757, 826, 1338, 1412, 1683, 1709
CspCI	CAANNNNNGTGG	2 cut(s)	28, 63
CviQI	GTAC	7 cut(s)	593, 757, 826, 1338, 1412, 1683, 1709
DdeI	CTNAG	6 cut(s)	499, 850, 875, 1065, 1431, 1869
DraIII	CACNNNGTG	1 cut(s)	1993
EaeI	YGGCCR	2 cut(s)	1893, 2148
Eam1104I	CTCTTC	7 cut(s)	224, 445, 690, 718, 1932, 2480, 2618
EarI	CTCTTC	7 cut(s)	224, 445, 690, 718, 1932, 2480, 2618
EciI	GGCGGA	2 cut(s)	143, 187
Eco147I	AGGCCT	1 cut(s)	1653
Eco24I	GRGCYC	1 cut(s)	490
Eco31I	GGTCTC	2 cut(s)	128, 2713
Eco47I	GGWCC	2 cut(s)	817, 2050
Eco57I	CTGAAG	3 cut(s)	528, 2121, 2340
Eco88I	CYCGRG	1 cut(s)	2585
EcoRI	GAATTC	1 cut(s)	1844
EcoRII	CCWGG	5 cut(s)	697, 832, 1216, 1517, 2182
EcoT38I	GRGCYC	1 cut(s)	490
FalI	AAGNNNNNCTT	8 cut(s)	579, 611, 833, 865, 2477, 2509, 2518, 2550
FaqI	GGGAC	2 cut(s)	1269, 1792
FauI	CCCGC	2 cut(s)	87, 199
FbaI	TGATCA	3 cut(s)	655, 1497, 1713
FblI	GTMKAC	3 cut(s)	261, 908, 1035
FokI	GGATG	5 cut(s)	815, 1001, 1684, 2513, 2605
FriOI	GRGCYC	1 cut(s)	490
FspBI	CTAG	8 cut(s)	1052, 1136, 1649, 1655, 2090, 2108, 2126, 2159
FspI	TGCGCA	1 cut(s)	1404
GlaI	GCGC	5 cut(s)	205, 968, 1296, 1404, 1962
GsuI	CTGGAG	4 cut(s)	796, 1589, 1707, 2673
HapII	CCGG	7 cut(s)	239, 914, 934, 1041, 1950, 2151, 2442
HgaI	GACGC	1 cut(s)	1286
HhaI	GCGC	5 cut(s)	206, 969, 1297, 1405, 1963
Hin6I	GCGC	5 cut(s)	204, 967, 1295, 1403, 1961
HinP1I	GCGC	5 cut(s)	204, 967, 1295, 1403, 1961
HincII	GTYRAC	6 cut(s)	262, 310, 640, 1131, 1810, 1876
HindII	GTYRAC	6 cut(s)	262, 310, 640, 1131, 1810, 1876
HpaI	GTTAAC	3 cut(s)	310, 1810, 1876
HpaII	CCGG	7 cut(s)	239, 914, 934, 1041, 1950, 2151, 2442
Hpy188I	TCNGA	8 cut(s)	114, 1081, 1237, 1390, 1434, 1455, 1928, 2170
Hpy188III	TCNNGA	7 cut(s)	248, 775, 1136, 1822, 1841, 2192, 2587
Hpy99I	CGWCG	1 cut(s)	266
HpyAV	CCTTC	9 cut(s)	47, 109, 214, 733, 1139, 1309, 1990, 2096, 2335
HpyCH4IV	ACGT	3 cut(s)	264, 392, 1152
HpyF3I	CTNAG	6 cut(s)	499, 850, 875, 1065, 1431, 1869
HpySE526I	ACGT	3 cut(s)	264, 392, 1152
HspAI	GCGC	5 cut(s)	204, 967, 1295, 1403, 1961
KroI	GCCGGC	1 cut(s)	2441
KroNI	GCCGGC	1 cut(s)	2443
Ksp22I	TGATCA	3 cut(s)	655, 1497, 1713
KspAI	GTTAAC	3 cut(s)	310, 1810, 1876
KspI	CCGCGG	2 cut(s)	61, 83
LguI	GCTCTTC	2 cut(s)	445, 2480
LmnI	GCTCC	6 cut(s)	200, 716, 886, 1450, 2654, 2728
LweI	GCATC	4 cut(s)	1072, 1706, 2065, 2397
MabI	ACCWGGT	1 cut(s)	2182
MaeI	CTAG	8 cut(s)	1052, 1136, 1649, 1655, 2090, 2108, 2126, 2159
MaeII	ACGT	3 cut(s)	264, 392, 1152
MaeIII	GTNAC	4 cut(s)	1985, 2224, 2239, 2246
MfeI	CAATTG	4 cut(s)	1788, 2251, 2433, 2457
MflI	RGATCY	3 cut(s)	628, 1614, 2589
MhlI	GDGCHC	9 cut(s)	155, 270, 283, 490, 1640, 1648, 1744, 2191, 2586
MlsI	TGGCCA	1 cut(s)	1895
MluNI	TGGCCA	1 cut(s)	1895
MlyI	GAGTC	7 cut(s)	167, 774, 1126, 1433, 1774, 1812, 2390
MmeI	TCCRAC	3 cut(s)	325, 1363, 1970
Mox20I	TGGCCA	1 cut(s)	1895
MroNI	GCCGGC	1 cut(s)	2441
MroXI	GAANNNNTTC	1 cut(s)	2534
MscI	TGGCCA	1 cut(s)	1895
MseI	TTAA	8 cut(s)	309, 600, 633, 806, 840, 995, 1809, 1875
MslI	CAYNNNNRTG	4 cut(s)	185, 395, 1269, 2237
Msp20I	TGGCCA	1 cut(s)	1895
MspA1I	CMGCKG	4 cut(s)	60, 82, 556, 1868
MspCI	CTTAAG	1 cut(s)	839
MspI	CCGG	7 cut(s)	239, 914, 934, 1041, 1950, 2151, 2442
MspR9I	CCNGG	8 cut(s)	240, 699, 834, 1042, 1218, 1519, 1951, 2184
MunI	CAATTG	4 cut(s)	1788, 2251, 2433, 2457
MvaI	CCWGG	5 cut(s)	699, 834, 1218, 1519, 2184
MvnI	CGCG	5 cut(s)	60, 82, 206, 249, 1297
NaeI	GCCGGC	1 cut(s)	2443
NciI	CCSGG	3 cut(s)	240, 1042, 1951
NcoI	CCATGG	4 cut(s)	813, 970, 1549, 1581
NgoMIV	GCCGGC	1 cut(s)	2441
NheI	GCTAGC	1 cut(s)	2107
NlaIV	GGNNCC	5 cut(s)	78, 154, 1110, 2440, 2591
NmuCI	GTSAC	3 cut(s)	1985, 2239, 2246
NruI	TCGCGA	1 cut(s)	249
NsbI	TGCGCA	1 cut(s)	1404
OliI	CACNNNNGTG	1 cut(s)	395
PasI	CCCWGGG	1 cut(s)	698
PceI	AGGCCT	1 cut(s)	1653
PciSI	GCTCTTC	2 cut(s)	445, 2480
PdiI	GCCGGC	1 cut(s)	2443
PdmI	GAANNNNTTC	1 cut(s)	2534
PfeI	GAWTC	5 cut(s)	244, 452, 901, 1942, 2122
PflMI	CCANNNNNTGG	1 cut(s)	2624
PfoI	TCCNGGA	1 cut(s)	1040
PleI	GAGTC	7 cut(s)	166, 773, 1126, 1432, 1774, 1812, 2390
PpsI	GAGTC	7 cut(s)	166, 773, 1126, 1432, 1774, 1812, 2390
PshBI	ATTAAT	1 cut(s)	600
PsiI	TTATAA	1 cut(s)	893
Psp6I	CCWGG	5 cut(s)	697, 832, 1216, 1517, 2182
PspGI	CCWGG	5 cut(s)	697, 832, 1216, 1517, 2182
PspN4I	GGNNCC	5 cut(s)	78, 154, 1110, 2440, 2591
PspPI	GGNCC	4 cut(s)	76, 817, 1952, 2050
PstI	CTGCAG	2 cut(s)	1867, 2605
PstNI	CAGNNNCTG	1 cut(s)	2099
PsuI	RGATCY	3 cut(s)	628, 1614, 2589
PvuII	CAGCTG	2 cut(s)	556, 1868
RruI	TCGCGA	1 cut(s)	249
RsaI	GTAC	7 cut(s)	594, 758, 827, 1339, 1413, 1684, 1710
RsaNI	GTAC	7 cut(s)	593, 757, 826, 1338, 1412, 1683, 1709
RseI	CAYNNNNRTG	4 cut(s)	185, 395, 1269, 2237
SacII	CCGCGG	2 cut(s)	61, 83
SalI	GTCGAC	1 cut(s)	260
SapI	GCTCTTC	2 cut(s)	445, 2480
SaqAI	TTAA	8 cut(s)	309, 600, 633, 806, 840, 995, 1809, 1875
Sau96I	GGNCC	4 cut(s)	76, 817, 1952, 2050
ScaI	AGTACT	1 cut(s)	758
SchI	GAGTC	7 cut(s)	167, 774, 1126, 1433, 1774, 1812, 2390
ScrFI	CCNGG	8 cut(s)	240, 699, 834, 1042, 1218, 1519, 1951, 2184
SduI	GDGCHC	9 cut(s)	155, 270, 283, 490, 1640, 1648, 1744, 2191, 2586
SexAI	ACCWGGT	1 cut(s)	2182
SfaNI	GCATC	4 cut(s)	1072, 1706, 2065, 2397
SfcI	CTRYAG	3 cut(s)	760, 1863, 2601
Sfr303I	CCGCGG	2 cut(s)	61, 83
SgrAI	CRCCGGYG	1 cut(s)	913
SgrBI	CCGCGG	2 cut(s)	61, 83
SinI	GGWCC	2 cut(s)	817, 2050
SmiMI	CAYNNNNRTG	4 cut(s)	185, 395, 1269, 2237
SmlI	CTYRAG	4 cut(s)	839, 2190, 2210, 2741
SmoI	CTYRAG	4 cut(s)	839, 2190, 2210, 2741
SseBI	AGGCCT	1 cut(s)	1653
SspMI	CTAG	8 cut(s)	1052, 1136, 1649, 1655, 2090, 2108, 2126, 2159
StuI	AGGCCT	1 cut(s)	1653
StyD4I	CCNGG	8 cut(s)	238, 697, 832, 1040, 1216, 1517, 1949, 2182
TaiI	ACGT	3 cut(s)	267, 395, 1155
TaqI	TCGA	5 cut(s)	261, 1767, 1840, 2073, 2763
TatI	WGTACW	3 cut(s)	756, 825, 1708
TauI	GCSGC	5 cut(s)	60, 63, 66, 85, 128
TfiI	GAWTC	5 cut(s)	244, 452, 901, 1942, 2122
Tru1I	TTAA	8 cut(s)	309, 600, 633, 806, 840, 995, 1809, 1875
Tru9I	TTAA	8 cut(s)	309, 600, 633, 806, 840, 995, 1809, 1875
TseFI	GTSAC	3 cut(s)	1985, 2239, 2246
Tsp45I	GTSAC	3 cut(s)	1985, 2239, 2246
TspGWI	ACGGA	2 cut(s)	166, 314
Van91I	CCANNNNNTGG	1 cut(s)	2624
Vha464I	CTTAAG	1 cut(s)	839
VneI	GTGCAC	1 cut(s)	1740
VpaK11BI	GGWCC	2 cut(s)	817, 2050
VspI	ATTAAT	1 cut(s)	600
XapI	RAATTY	6 cut(s)	747, 1478, 1544, 1844, 1922, 2266
XbaI	TCTAGA	1 cut(s)	1135
XcmI	CCANNNNNNNNNTGG	1 cut(s)	145
XmiI	GTMKAC	3 cut(s)	261, 908, 1035
XmnI	GAANNNNTTC	1 cut(s)	2534
XspI	CTAG	8 cut(s)	1052, 1136, 1649, 1655, 2090, 2108, 2126, 2159
ZrmI	AGTACT	1 cut(s)	758

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.