Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0002025

Physical Location & Seq

Genomic Coordinates Forward (+)

53963228 .. 53972263

Gene Structure 9036 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000003885

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2982 bp

ATGGCGACCAAGTTCATTGCTCTTGCGTGCATACGCAACAACAACAGTGGTGAAGGCCGCGGCGGCAGTGATTTGTCGCCGCGGCCTCACTATCCTTCCATGCCAAAGTATCCGAAGGGTGTGGTGGCGGAGGAGACCACTATGGTGGTGGGCACCGAGTCCAAGGCCGTGTTCTCCGTCATCGGCATGACCTGCTCCGCCTGCGCGGGATCGGTAGAGAAGGCTGTGAAGAGGCTACCCGGGATTCGTGAGGCGGTGGTCGACGTGCTCAACAACCGTGCCCAAGTCATGTTCTTCCCCAATTTCGTTAACGCAGAGATTATCCGTGAGACTATTGAAGATGTTGGATTTCAAGCAACATTGATTGCTGATGAGGGAAACGAGAAGTCCACGTTGGTGTGCAGAATAAGGATCAAAGGAATGACTTGCACTTCTTGCTCTTCCACTGTTGAATCAGCTTTGCAGGCAGTTCATGGTGTACAAAAAGCCCAAGTTGCCTTAGCAACTGAAGAAGCAGATGTCCACTATGATCCAAAGATAGTGAGCTGCAATCAGCTGATGGTAGCCATTGAAGACACTGGATTCGAAGCCATACTTATTAATTCAGGTGAAGACATGAGCAAGATAGATCTTAAAGTTGACGGTGTAAGCACTGATCATTCAATGAGAATACTTGAACAGTCTCTTCAAGCACTCCCTGGGGTGCAAGGTGTGGACATAAATCATGATGACAGAAAAATTTCCCTGTCTTACAAGCCAGATATGACAGGGCCCCGAAATTTCATCAATGTGATTGAAACAACTGGATCCAGGCGTTTCAGGGCAAAAATTTATCCAGGAGGTGGAGCAGGAAGAGAAAATCATAGGAACGAGGAAATTGAGCAGTACTACAGAGTCTTTCTCTGGAGTTTGATTTTCACCATTCCAGTATTTTTAACCTCCATGGTCTTCATGTACATCCCTGGACTTAAGCATGGCTTAGACAAGAAAGTAGTAAATATGCTCAGCATTGGGGAGCTTATAAGGTGGATTCTGTCTACTCCGGTGCAGTTCATCATAGGCCGGAGGTTCTATACTGGGGCATACAAGTCATTGCGCCATGGTTCTGCTAATATGGATGTGTTAATTGCCTTGGGAACAAATGCAGCATATTTCTACTCAGTCTACTCTGTACTGAGAGCTGCCACCTCCCCACATTTTAAGGGTACAGATTTCTTTGAGACTAGTGCAATGCTAATCTCATTCATTCTCTTAGGGAAGTACCTAGAGGTGTTAGCTAAGGGGAAGACATCAGATGCTATTGCCAAACTGATGGATTTGGCACCTGAAACAGCCACTTTGTTGACTCTAGACGAAGAAGGAAACATTTTAGGTGAAGAAGAAATTGATGGTCGGTTGATACAAAAAAACGATGTCATCAAAATTATTCCAGGGGCGAAGGTAGCTTCGGATGGTTATGTGATATGGGGACAAAGCCATGTAAATGAGAGTATGATAACAGGAGAAGCGCGTCCAGTGGCAAAAAGGAAGGGTGATACAGTCATTGGAGGTACTGTAAATGAAAATGGTGTGTTGCATATCAAGGCAACACGGGTTGGCTCAGAAAGTTCCCTCGCGCAGATTGTACGGCTTGTTGAGTCAGCTCAGATGGCAAAAGCTCCTGTTCAGAAGTTTGCTGACCGCATTTCTAAATTCTTTGTGCCACTGGTGATCATGCTCTCATTCTTTACCTGGCTTTCATGGTTTTTGGCTGGAAAATTCCATGGCTACCCGAAATCTTGGATACCGAAGTCCATGGATAGCTTTCAGCTTGCTCTCCAGTTTGGGATCTCTGTCATGGTTATTGCGTGCCCTTGTGCTCTAGGCCTAGCAACTCCTACTGCTGTCATGGTTGGTACTGGAGTCGGTGCATCCCAAGGTGTACTGATCAAAGGAGGTCAAGCATTAGAAAGTGCACATAAGGTGGACTGCATTGTGTTCGACAAGACAGGGACTCTTACAATTGGGAAGCCACTAGTTGTTAACACACGACTCCTGAAAAATATGGTGCTTCGAGAATTCTATGAACTCGTTGCTGCAGCTGAGGTTAACAGTGAACACCCCTTGGCCAAGGCCATTGTTGAGTATGCCAAAAAATTCAGAGAAGATGAAGCGAATCCAGCCGGGCCAGAAGCGCATGACTTTGCCTCCATTACTGGTCACGGGGTGAAGGCTATTGTTCGGGGCAGGGAAATAATTGTGGGGAACAAGAGCTTGATGGTGGACCAGAACATTACAGTTCCACTCGATGCAGAAGACTACCTAGCTGAAGCTGAAGGGCTGGCTCAAACTGGGATTCTAGTAGCCATAGATGGAGAAGTGGCCGGAGTTCTAGCAATATCTGATCCACTGAAACCAGGTGCTCAAGAAGTAATTACCATACTCAAGTCAATGAATGTTAGAAGCATAATGGTGACAGGTGACAATTGGGGAACTGCAAATTCTATTGCCAATGAAGTTGGTATTGATACTGTTATAGCCGAAGCGAAACCTGACCAGAAAGCAGAGAAAGTGAAGGAATTGCAGGCTTCAGGCTACACTGTGGCGATGGTGGGAGATGGCATCAATGACTCACCAGCACTTGTGGCGGCAGATGTAGGAATGGCAATTGGTGCTGGCACTGACATTGCAATTGAGGCAGCAGACATTGTGCTAATGAAGAGCAACTTGGAGGATGTGATAACTGCCATTGATCTTTCAAGGAAAACCTTCACCCGTATTCGTTTGAACTACATTTGGGCTTTGGGATATAATGTGCTCGGGATCCCAATAGCTGCAGGAGTTCCCTTCCCATCCACTGGATTTCGCTTACCCCCTTGGATTGCTGGAGCTGCAATGGCTGCTTCTTCTGTCAGTGTTGTTTGCTGTTCATTGTTGTTGAAGAACTATAAGAGACCCAAGGAGCTGAATAACCTTGAGGTGCGTGGAATAGTGATCGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0008150 GO:0008324 GO:0009636 GO:0010035 GO:0010038 GO:0010273 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042221 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046688 GO:0046873 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0061687 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0097501 GO:0098655 GO:0098660 GO:0098662 GO:0098754 GO:0099131 GO:0099132 GO:1990169

KEGG Pathways

Metabolic & Signaling

ko01524 ko04016 map01524 map04016

Pfam Domains

Protein Families

E1-E2_ATPase HMA Hydrolase

Protein Analysis

994

Amino Acids

107.19

Weight (kDa)

6.07

Isoelectric Point (pI)

29.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMA	PF00403	57 - 117	2.4e-13	Heavy-metal-associated domain
HMA	PF00403	136 - 196	3.2e-13	Heavy-metal-associated domain
E1-E2_ATPase	PF00122	440 - 546	2.1e-23	P-type ATPase actuator domain
Hydrolase	PF00702	655 - 878	1.4e-34	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0002025)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G63440
fragaria_vesca	FvH4_5g02090 FvH4_5g02090 FvH4_5g02091 FvH4_5g02091 FvH4_5g02091 FvH4_5g02091
malus_domestica	MD06G1080800.v1.1 MD06G1080900.v1.1 MD14G1102600.v1.1
prunus_persica	Prupe.5G097900_v2.0.a1 Prupe.5G097900_v2.0.a1 Prupe.5G098000_v2.0.a1
pyrus_communis	pycom06g07780 pycom06g07790 pycom14g09890
rosa_chinensis	RchiOBHm_Chr7g0197781 RchiOBHm_Chr7g0198111 RchiOBHm_Chr7g0198141
rosa_laevigata	RLG00000003885 RLG00000003888 RLG00000019170
rosa_multiflora	Rmu_sc0000221.1_g000018 Rmu_sc0000221.1_g000030 Rmu_sc0006732.1_g000016
rosa_roxburghii	Rroxscaffold_3G00257940 Rroxscaffold_3G00257970 Rroxscaffold_3G00257990
rosa_rugosa	Rorug07G0045700 Rorug07G0045700 Rorug07G0045800 Rorug07G0045800 Rorug07G0045900
rosa_samantha	Rh7AG169100 Rh7AG169200 Rh7BG169900 Rh7BG172300 Rh7CG178600 Rh7CG178700 Rh7DG169200 Rh7DG171400 Rh7DG171800
rosa_wichuraiana	Rw7G014570 Rw7G014780 Rw7G014790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1022
Acc36I	ACCTGC	1 cut(s)	200
AccB1I	GGYRCC	2 cut(s)	152, 1321
AccB7I	CCANNNNNTGG	2 cut(s)	842, 2837
AccI	GTMKAC	3 cut(s)	261, 1037, 1164
AccII	CGCG	5 cut(s)	60, 82, 206, 1510, 1616
AclWI	GGATC	9 cut(s)	217, 419, 524, 801, 814, 1835, 2378, 2797, 2810
AcoI	YGGCCR	2 cut(s)	2106, 2361
AcsI	RAATTY	8 cut(s)	738, 778, 828, 1691, 1757, 2057, 2135, 2479
AcuI	CTGAAG	4 cut(s)	528, 2328, 2334, 2553
AdeI	CACNNNGTG	1 cut(s)	2206
AfiI	CCNNNNNNNGG	2 cut(s)	842, 2837
AflII	CTTAAG	1 cut(s)	968
AhlI	ACTAGT	2 cut(s)	1223, 2014
AjiI	CACGTC	1 cut(s)	265
AjnI	CCWGG	7 cut(s)	697, 809, 835, 961, 1429, 1730, 2395
AjuI	GAANNNNNNNTTGG	6 cut(s)	155, 187, 377, 409, 2690, 2722
AleI	CACNNNNGTG	2 cut(s)	143, 395
Alw21I	GWGCWC	5 cut(s)	270, 1861, 1957, 2404, 2799
Alw26I	GTCTC	5 cut(s)	128, 323, 687, 1214, 2926
Alw44I	GTGCAC	1 cut(s)	1953
AlwI	GGATC	9 cut(s)	217, 419, 524, 801, 814, 1835, 2378, 2797, 2810
Ama87I	CYCGRG	2 cut(s)	239, 2798
ApaI	GGGCCC	1 cut(s)	774
ApaLI	GTGCAC	1 cut(s)	1953
ApeKI	GCWGC	9 cut(s)	546, 1145, 1181, 2075, 2078, 2678, 2813, 2870, 2879
ApoI	RAATTY	8 cut(s)	738, 778, 828, 1691, 1757, 2057, 2135, 2479
ArsI	GACNNNNNNTTYG	2 cut(s)	566, 598
AseI	ATTAAT	1 cut(s)	600
Asp700I	GAANNNNTTC	2 cut(s)	739, 2747
AspLEI	GCGC	5 cut(s)	206, 1098, 1510, 1618, 2176
AspS9I	GGNCC	4 cut(s)	770, 771, 2165, 2263
AsuC2I	CCSGG	3 cut(s)	240, 241, 2164
AsuII	TTCGAA	1 cut(s)	585
AvaI	CYCGRG	2 cut(s)	239, 2798
AvaII	GGWCC	1 cut(s)	2263
BaeGI	GKGCMC	5 cut(s)	155, 283, 774, 1853, 1957
BaeI	ACNNNNGTAYC	4 cut(s)	1775, 1808, 1887, 1920
BalI	TGGCCA	1 cut(s)	2108
BamHI	GGATCC	2 cut(s)	806, 2802
BanI	GGYRCC	2 cut(s)	152, 1321
BanII	GRGCYC	1 cut(s)	774
BbsI	GAAGAC	5 cut(s)	579, 618, 940, 1292, 2301
Bbv12I	GWGCWC	5 cut(s)	270, 1861, 1957, 2404, 2799
BbvCI	CCTCAGC	1 cut(s)	2082
BbvI	GCAGC	9 cut(s)	533, 1157, 1168, 2062, 2090, 2690, 2800, 2857, 2866
BceAI	ACGGC	2 cut(s)	152, 1643
BcgI	CGANNNNNNTGC	2 cut(s)	1960, 1994
BciT130I	CCWGG	7 cut(s)	699, 811, 837, 963, 1431, 1732, 2397
BciVI	GTATCC	2 cut(s)	120, 1776
BclI	TGATCA	3 cut(s)	655, 1710, 1926
BcnI	CCSGG	3 cut(s)	240, 241, 2164
BcoDI	GTCTC	5 cut(s)	128, 323, 687, 1214, 2926
BcuI	ACTAGT	2 cut(s)	1223, 2014
BfaI	CTAG	9 cut(s)	1224, 1265, 1349, 1862, 1868, 2015, 2303, 2339, 2372
BfmI	CTRYAG	3 cut(s)	889, 2076, 2814
BfrI	CTTAAG	1 cut(s)	968
BfuAI	ACCTGC	1 cut(s)	200
BfuI	GTATCC	2 cut(s)	120, 1776
BglI	GCCNNNNNGGC	1 cut(s)	63
BglII	AGATCT	1 cut(s)	628
BlpI	GCTNAGC	1 cut(s)	1004
BmcAI	AGTACT	1 cut(s)	887
Bme18I	GGWCC	1 cut(s)	2263
BmeT110I	CYCGRG	2 cut(s)	239, 2798
BmgBI	CACGTC	1 cut(s)	265
BmgT120I	GGNCC	4 cut(s)	770, 771, 2165, 2263
BmiI	GGNNCC	6 cut(s)	154, 772, 773, 808, 1323, 2804
BmrI	ACTGGG	2 cut(s)	1086, 2340
BmsI	GCATC	4 cut(s)	1285, 1919, 2278, 2610
BmuI	ACTGGG	2 cut(s)	1086, 2340
BpiI	GAAGAC	5 cut(s)	579, 618, 940, 1292, 2301
BplI	GAGNNNNNCTC	2 cut(s)	885, 917
BpmI	CTGGAG	4 cut(s)	925, 1802, 1920, 2886
Bpu10I	CCTNAGC	3 cut(s)	499, 1278, 2082
Bpu1102I	GCTNAGC	1 cut(s)	1004
Bpu14I	TTCGAA	1 cut(s)	585
BpuEI	CTTGAG	3 cut(s)	2388, 2408, 2975
BpuMI	CCSGG	3 cut(s)	240, 241, 2164
BsaI	GGTCTC	2 cut(s)	128, 2926
BsaWI	WCCGGW	1 cut(s)	1042
BsaXI	ACNNNNNCTCC	4 cut(s)	1539, 1569, 2171, 2201
Bsc4I	CCNNNNNNNGG	2 cut(s)	842, 2837
Bse3DI	GCAATG	5 cut(s)	15, 1091, 1236, 2664, 2880
BseBI	CCWGG	7 cut(s)	699, 811, 837, 963, 1431, 1732, 2397
BseGI	GGATG	6 cut(s)	957, 1123, 1456, 1910, 2719, 2831
BseLI	CCNNNNNNNGG	2 cut(s)	842, 2837
BseMI	GCAATG	5 cut(s)	15, 1091, 1236, 2664, 2880
BseMII	CTCAG	6 cut(s)	1018, 1166, 1173, 1614, 1658, 2073
BseRI	GAGGAG	1 cut(s)	146
BseSI	GKGCMC	5 cut(s)	155, 283, 774, 1853, 1957
BseXI	GCAGC	9 cut(s)	533, 1157, 1168, 2062, 2090, 2690, 2800, 2857, 2866
BsgI	GTGCAG	2 cut(s)	421, 1067
Bsh1236I	CGCG	5 cut(s)	60, 82, 206, 1510, 1616
BshNI	GGYRCC	2 cut(s)	152, 1321
BsiHKAI	GWGCWC	5 cut(s)	270, 1861, 1957, 2404, 2799
BsiHKCI	CYCGRG	2 cut(s)	239, 2798
BsiSI	CCGG	5 cut(s)	240, 1043, 1063, 2163, 2364
BslFI	GGGAC	2 cut(s)	1482, 2005
BslI	CCNNNNNNNGG	2 cut(s)	842, 2837
BsmAI	GTCTC	5 cut(s)	128, 323, 687, 1214, 2926
BsmFI	GGGAC	2 cut(s)	1482, 2005
Bso31I	GGTCTC	2 cut(s)	128, 2926
BsoBI	CYCGRG	2 cut(s)	239, 2798
Bsp119I	TTCGAA	1 cut(s)	585
Bsp120I	GGGCCC	1 cut(s)	770
Bsp1286I	GDGCHC	9 cut(s)	155, 270, 283, 774, 1853, 1861, 1957, 2404, 2799
Bsp1407I	TGTACA	2 cut(s)	478, 954
Bsp1720I	GCTNAGC	1 cut(s)	1004
Bsp19I	CCATGG	4 cut(s)	942, 1099, 1762, 1794
BspCNI	CTCAG	6 cut(s)	1017, 1167, 1172, 1613, 1657, 2074
BspFNI	CGCG	5 cut(s)	60, 82, 206, 1510, 1616
BspHI	TCATGA	1 cut(s)	724
BspLI	GGNNCC	6 cut(s)	154, 772, 773, 808, 1323, 2804
BspMAI	CTGCAG	2 cut(s)	2080, 2818
BspMI	ACCTGC	1 cut(s)	200
BspPI	GGATC	9 cut(s)	217, 419, 524, 801, 814, 1835, 2378, 2797, 2810
BspQI	GCTCTTC	2 cut(s)	445, 2693
BspT104I	TTCGAA	1 cut(s)	585
BspT107I	GGYRCC	2 cut(s)	152, 1321
BspTI	CTTAAG	1 cut(s)	968
BspTNI	GGTCTC	2 cut(s)	128, 2926
BsrDI	GCAATG	5 cut(s)	15, 1091, 1236, 2664, 2880
BsrGI	TGTACA	2 cut(s)	478, 954
Bst2UI	CCWGG	7 cut(s)	699, 811, 837, 963, 1431, 1732, 2397
Bst6I	CTCTTC	5 cut(s)	224, 445, 690, 847, 2693
BstAFI	CTTAAG	1 cut(s)	968
BstAPI	GCANNNNNTGC	4 cut(s)	192, 435, 2651, 2879
BstAUI	TGTACA	2 cut(s)	478, 954
BstBI	TTCGAA	1 cut(s)	585
BstC8I	GCNNGC	8 cut(s)	28, 202, 465, 1812, 1849, 2322, 2565, 2656
BstDSI	CCRYGG	6 cut(s)	58, 80, 942, 1099, 1762, 1794
BstF5I	GGATG	6 cut(s)	957, 1123, 1456, 1910, 2719, 2831
BstFNI	CGCG	5 cut(s)	60, 82, 206, 1510, 1616
BstHHI	GCGC	5 cut(s)	206, 1098, 1510, 1618, 2176
BstMAI	GTCTC	5 cut(s)	128, 323, 687, 1214, 2926
BstNI	CCWGG	7 cut(s)	699, 811, 837, 963, 1431, 1732, 2397
BstSFI	CTRYAG	3 cut(s)	889, 2076, 2814
BstSLI	GKGCMC	5 cut(s)	155, 283, 774, 1853, 1957
BstUI	CGCG	5 cut(s)	60, 82, 206, 1510, 1616
BstV1I	GCAGC	9 cut(s)	533, 1157, 1168, 2062, 2090, 2690, 2800, 2857, 2866
BstV2I	GAAGAC	5 cut(s)	579, 618, 940, 1292, 2301
BstX2I	RGATCY	4 cut(s)	628, 806, 1827, 2802
BstXI	CCANNNNNNTGG	2 cut(s)	145, 1312
BstYI	RGATCY	4 cut(s)	628, 806, 1827, 2802
BsuI	GTATCC	2 cut(s)	120, 1776
BtgI	CCRYGG	6 cut(s)	58, 80, 942, 1099, 1762, 1794
BtgZI	GCGATG	1 cut(s)	2600
BtrI	CACGTC	1 cut(s)	265
BtsCI	GGATG	6 cut(s)	957, 1123, 1456, 1910, 2719, 2831
BtsI	GCAGTG	1 cut(s)	73
BveI	ACCTGC	1 cut(s)	200
Cac8I	GCNNGC	8 cut(s)	28, 202, 465, 1812, 1849, 2322, 2565, 2656
CciI	TCATGA	1 cut(s)	724
CfoI	GCGC	5 cut(s)	206, 1098, 1510, 1618, 2176
Cfr13I	GGNCC	4 cut(s)	770, 771, 2165, 2263
Cfr42I	CCGCGG	2 cut(s)	61, 83
Cfr9I	CCCGGG	1 cut(s)	239
CseI	GACGC	1 cut(s)	1499
CsiI	ACCWGGT	1 cut(s)	2395
CspCI	CAANNNNNGTGG	2 cut(s)	28, 63
DraIII	CACNNNGTG	1 cut(s)	2206
EaeI	YGGCCR	2 cut(s)	2106, 2361
Eam1104I	CTCTTC	5 cut(s)	224, 445, 690, 847, 2693
EarI	CTCTTC	5 cut(s)	224, 445, 690, 847, 2693
EciI	GGCGGA	2 cut(s)	143, 187
Eco147I	AGGCCT	1 cut(s)	1866
Eco24I	GRGCYC	1 cut(s)	774
Eco31I	GGTCTC	2 cut(s)	128, 2926
Eco47I	GGWCC	1 cut(s)	2263
Eco57I	CTGAAG	4 cut(s)	528, 2328, 2334, 2553
Eco88I	CYCGRG	2 cut(s)	239, 2798
EcoO109I	RGGNCCY	2 cut(s)	770, 771
EcoRI	GAATTC	1 cut(s)	2057
EcoRII	CCWGG	7 cut(s)	697, 809, 835, 961, 1429, 1730, 2395
EcoT38I	GRGCYC	1 cut(s)	774
FalI	AAGNNNNNCTT	6 cut(s)	579, 611, 962, 994, 2690, 2722
FaqI	GGGAC	2 cut(s)	1482, 2005
FauI	CCCGC	1 cut(s)	199
FbaI	TGATCA	3 cut(s)	655, 1710, 1926
FblI	GTMKAC	3 cut(s)	261, 1037, 1164
FokI	GGATG	6 cut(s)	944, 1130, 1463, 1897, 2726, 2818
FriOI	GRGCYC	1 cut(s)	774
FspBI	CTAG	9 cut(s)	1224, 1265, 1349, 1862, 1868, 2015, 2303, 2339, 2372
GlaI	GCGC	5 cut(s)	205, 1097, 1509, 1617, 2175
GsuI	CTGGAG	4 cut(s)	925, 1802, 1920, 2886
HapII	CCGG	5 cut(s)	240, 1043, 1063, 2163, 2364
HgaI	GACGC	1 cut(s)	1499
HhaI	GCGC	5 cut(s)	206, 1098, 1510, 1618, 2176
Hin6I	GCGC	5 cut(s)	204, 1096, 1508, 1616, 2174
HinP1I	GCGC	5 cut(s)	204, 1096, 1508, 1616, 2174
HincII	GTYRAC	6 cut(s)	262, 310, 640, 1344, 2023, 2089
HindII	GTYRAC	6 cut(s)	262, 310, 640, 1344, 2023, 2089
HpaI	GTTAAC	3 cut(s)	310, 2023, 2089
HpaII	CCGG	5 cut(s)	240, 1043, 1063, 2163, 2364
Hpy188I	TCNGA	8 cut(s)	114, 1294, 1450, 1603, 1647, 1668, 2141, 2383
Hpy188III	TCNNGA	8 cut(s)	248, 725, 904, 1349, 2035, 2054, 2405, 2800
Hpy99I	CGWCG	1 cut(s)	266
HpyCH4IV	ACGT	2 cut(s)	264, 392
HpySE526I	ACGT	2 cut(s)	264, 392
HspAI	GCGC	5 cut(s)	204, 1096, 1508, 1616, 2174
Ksp22I	TGATCA	3 cut(s)	655, 1710, 1926
KspAI	GTTAAC	3 cut(s)	310, 2023, 2089
KspI	CCGCGG	2 cut(s)	61, 83
LguI	GCTCTTC	2 cut(s)	445, 2693
LmnI	GCTCC	6 cut(s)	200, 845, 1015, 1663, 2867, 2941
Lsp1109I	GCAGC	9 cut(s)	533, 1157, 1168, 2062, 2090, 2690, 2800, 2857, 2866
LweI	GCATC	4 cut(s)	1285, 1919, 2278, 2610
MabI	ACCWGGT	1 cut(s)	2395
MaeI	CTAG	9 cut(s)	1224, 1265, 1349, 1862, 1868, 2015, 2303, 2339, 2372
MaeII	ACGT	2 cut(s)	264, 392
MaeIII	GTNAC	3 cut(s)	2198, 2452, 2459
MfeI	CAATTG	4 cut(s)	2001, 2464, 2646, 2670
MflI	RGATCY	4 cut(s)	628, 806, 1827, 2802
MhlI	GDGCHC	9 cut(s)	155, 270, 283, 774, 1853, 1861, 1957, 2404, 2799
MlsI	TGGCCA	1 cut(s)	2108
MluNI	TGGCCA	1 cut(s)	2108
MlyI	GAGTC	8 cut(s)	167, 903, 1339, 1646, 1911, 1987, 2025, 2603
MmeI	TCCRAC	1 cut(s)	325
Mox20I	TGGCCA	1 cut(s)	2108
MroXI	GAANNNNTTC	2 cut(s)	739, 2747
MscI	TGGCCA	1 cut(s)	2108
MseI	TTAA	9 cut(s)	309, 600, 633, 935, 969, 1124, 1200, 2022, 2088
MslI	CAYNNNNRTG	6 cut(s)	143, 185, 395, 788, 1482, 2450
Msp20I	TGGCCA	1 cut(s)	2108
MspA1I	CMGCKG	4 cut(s)	60, 82, 556, 2081
MspCI	CTTAAG	1 cut(s)	968
MspI	CCGG	5 cut(s)	240, 1043, 1063, 2163, 2364
MunI	CAATTG	4 cut(s)	2001, 2464, 2646, 2670
MvaI	CCWGG	7 cut(s)	699, 811, 837, 963, 1431, 1732, 2397
MvnI	CGCG	5 cut(s)	60, 82, 206, 1510, 1616
NciI	CCSGG	3 cut(s)	240, 241, 2164
NcoI	CCATGG	4 cut(s)	942, 1099, 1762, 1794
NlaIV	GGNNCC	6 cut(s)	154, 772, 773, 808, 1323, 2804
NmuCI	GTSAC	3 cut(s)	2198, 2452, 2459
NspV	TTCGAA	1 cut(s)	585
OliI	CACNNNNGTG	2 cut(s)	143, 395
PagI	TCATGA	1 cut(s)	724
PasI	CCCWGGG	1 cut(s)	698
PceI	AGGCCT	1 cut(s)	1866
PciSI	GCTCTTC	2 cut(s)	445, 2693
PdmI	GAANNNNTTC	2 cut(s)	739, 2747
PfeI	GAWTC	6 cut(s)	244, 452, 582, 1030, 2155, 2335
PflMI	CCANNNNNTGG	2 cut(s)	842, 2837
PfoI	TCCNGGA	1 cut(s)	835
PleI	GAGTC	8 cut(s)	166, 902, 1339, 1645, 1910, 1987, 2025, 2603
PpsI	GAGTC	8 cut(s)	166, 902, 1339, 1645, 1910, 1987, 2025, 2603
PshBI	ATTAAT	1 cut(s)	600
PsiI	TTATAA	1 cut(s)	1022
Psp6I	CCWGG	7 cut(s)	697, 809, 835, 961, 1429, 1730, 2395
PspGI	CCWGG	7 cut(s)	697, 809, 835, 961, 1429, 1730, 2395
PspN4I	GGNNCC	6 cut(s)	154, 772, 773, 808, 1323, 2804
PspOMI	GGGCCC	1 cut(s)	770
PspPI	GGNCC	4 cut(s)	770, 771, 2165, 2263
PstI	CTGCAG	2 cut(s)	2080, 2818
PsuI	RGATCY	4 cut(s)	628, 806, 1827, 2802
PvuII	CAGCTG	2 cut(s)	556, 2081
RseI	CAYNNNNRTG	6 cut(s)	143, 185, 395, 788, 1482, 2450
SacII	CCGCGG	2 cut(s)	61, 83
SalI	GTCGAC	1 cut(s)	260
SapI	GCTCTTC	2 cut(s)	445, 2693
SaqAI	TTAA	9 cut(s)	309, 600, 633, 935, 969, 1124, 1200, 2022, 2088
Sau96I	GGNCC	4 cut(s)	770, 771, 2165, 2263
ScaI	AGTACT	1 cut(s)	887
SchI	GAGTC	8 cut(s)	167, 903, 1339, 1646, 1911, 1987, 2025, 2603
SduI	GDGCHC	9 cut(s)	155, 270, 283, 774, 1853, 1861, 1957, 2404, 2799
SexAI	ACCWGGT	1 cut(s)	2395
SfaNI	GCATC	4 cut(s)	1285, 1919, 2278, 2610
SfcI	CTRYAG	3 cut(s)	889, 2076, 2814
Sfr303I	CCGCGG	2 cut(s)	61, 83
SfuI	TTCGAA	1 cut(s)	585
SgrBI	CCGCGG	2 cut(s)	61, 83
SinI	GGWCC	1 cut(s)	2263
SmaI	CCCGGG	1 cut(s)	241
SmiMI	CAYNNNNRTG	6 cut(s)	143, 185, 395, 788, 1482, 2450
SmlI	CTYRAG	4 cut(s)	968, 2403, 2423, 2954
SmoI	CTYRAG	4 cut(s)	968, 2403, 2423, 2954
SpeI	ACTAGT	2 cut(s)	1223, 2014
SseBI	AGGCCT	1 cut(s)	1866
SspMI	CTAG	9 cut(s)	1224, 1265, 1349, 1862, 1868, 2015, 2303, 2339, 2372
StuI	AGGCCT	1 cut(s)	1866
TaiI	ACGT	2 cut(s)	267, 395
TaqI	TCGA	6 cut(s)	261, 585, 1980, 2053, 2286, 2976
TatI	WGTACW	5 cut(s)	478, 885, 954, 1171, 1921
TauI	GCSGC	6 cut(s)	60, 63, 66, 82, 85, 2630
TfiI	GAWTC	6 cut(s)	244, 452, 582, 1030, 2155, 2335
Tru1I	TTAA	9 cut(s)	309, 600, 633, 935, 969, 1124, 1200, 2022, 2088
Tru9I	TTAA	9 cut(s)	309, 600, 633, 935, 969, 1124, 1200, 2022, 2088
TseFI	GTSAC	3 cut(s)	2198, 2452, 2459
TseI	GCWGC	9 cut(s)	546, 1145, 1181, 2075, 2078, 2678, 2813, 2870, 2879
Tsp45I	GTSAC	3 cut(s)	2198, 2452, 2459
TspGWI	ACGGA	2 cut(s)	166, 314
TspMI	CCCGGG	1 cut(s)	239
Van91I	CCANNNNNTGG	2 cut(s)	842, 2837
Vha464I	CTTAAG	1 cut(s)	968
VneI	GTGCAC	1 cut(s)	1953
VpaK11BI	GGWCC	1 cut(s)	2263
VspI	ATTAAT	1 cut(s)	600
XapI	RAATTY	8 cut(s)	738, 778, 828, 1691, 1757, 2057, 2135, 2479
XbaI	TCTAGA	1 cut(s)	1348
XcmI	CCANNNNNNNNNTGG	1 cut(s)	145
XmaI	CCCGGG	1 cut(s)	239
XmiI	GTMKAC	3 cut(s)	261, 1037, 1164
XmnI	GAANNNNTTC	2 cut(s)	739, 2747
XspI	CTAG	9 cut(s)	1224, 1265, 1349, 1862, 1868, 2015, 2303, 2339, 2372
ZrmI	AGTACT	1 cut(s)	887

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.