Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0002025

Physical Location & Seq

Genomic Coordinates Forward (+)

15859047 .. 15864307

Gene Structure 5261 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1102600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3006 bp

ATGGCGACCAAGTTCTTGGCCCTATGCATACGCAACGAGAGCTGGGGTGACCTCTCACCCCGGCCCCACTACCCCTCCATGCCAAAGTACCCCAAAGGTGTGGCGGCTGAAGAGACCAGCCTGGCAGCCGGAGCTGAGGATAAGGCTTTGTTCTCTGTCACGGGCATGACGTGCTCCGCCTGCGCCGGGTCAGTCGAGAAGGCCGTCAAGAGGCTCCCGGGGATTCGCGAGGCTATTGTCGACGTCTTGAACAACCGGGCGCAAGTTATGTTCTTTCCCAATTACGTTAACGCGGAGACTATTCGCGAGACGATTGAAGATGTTGGATTCCAAGCCACTTTGATTAATGATGAGGAGGGAAATGAGAAGTCCACATTGATATGCAGAATTCTGATAAAGGGAATGACTTGCACTTCTTGCTCGACCACTGTTGAATCAGCTTTACAGGCAGTTGATGGTGTACAAAAAGCCCAAGTTGCCTTAGCAACTGAAGAAGCAGATGTTCACTATGATCCAAAGATTGTGAGCTACAACCAGCTGTTGCAAACCATTGAAGACACTGGATTCGAAGGCAAACTCATTACTGCAGGGGAACACATGAGCCGGATAGAGCTTGAAGTTGATGGTGTAAGGACCGATCGTTCAATGAGGATACTTGAACAGTCTCTTCAAGCACTCCCTGGTGTGCAAGCTGTAGACTTCGATTCTGAAATCAAGAAAATCTCTCTTTATTACAAATCAGATATTACAGGGCCAAGAAGTTTCATTAATGTGATTGAAACAACCGGGTCCAGGCGTTTCAAGGCAAGGATTTTTCCAGGAGGTGAAGCAGGAAGAGATAGTCATAGAAAGGAGGAAATTAAGCAGTATTTTAGAGTCTTTCTGTGGAGTTTGGTTTTCACTATTCCGGTGTTTTTAACCTCCATGGTCTTCATGTATATTCCTGGAATTAAGCATGGCCTAGAAACTAAAATTGTGAATAATCTCATGATTGGGGAGCTCATGAGATGGATTCTGGCCACCCCGGTGCAATTCATTATAGGCCAGAGGTTCTATACTGGGGCATACAAATCACTGCGCCATGGTTCCGCCAATATGGATGTGCTAATCGCATTAGGAACAAATGCAGCCTATTTTTATTCGGTCTACTCGGTAGTGAGAGCTGCTACCTCTCCAGATTTTATGGGGACTGATTTCTTCGAGACTAGTGCCATGCTTATTTCATTCATTCTCCTTGGAAAGTACCTAGAGGTATTAACTAAGGGGAAGACATCTGATGCCATTGCCAAATTGATGGACTTGGCACCTGAAACAGCGGCATTGTTGGCACTAGATGAAGTAGGAAATGTGATAAATGAACAGGAAATTGATAGTAGGTTGATACAAAAGAATGACATACTTAAAATTATTCCAGGGGCGAAAGTAGCTTCAGATGGTTATGTTACATGGGGGCAAAGCCATGTTAATGAGAGTATGATAACAGGAGAAGCACGACCAGTCGCAAAAAGAAAGGGTGACTCGGTCATTGGAGGTACTTTGAATGAGAATGGTGTCCTACATATCAAGGCAACTAGAGTTGGTTCAGAAAGTTCCCTTTCCCAGATTGTACGGCTGGTTGAGTCAGCTCAGATGGCCAAAGCTCCTGTACAGAAGTTTGCTGACCGCATTTCCAAATACTTTGTGCCACTGGATACCAGAGGCTTTTTCTTAAGACGTGGCTCTAATTTGCAGGTGATCCTACTTTCATTCTTGACCTGGCTTTCCTGGTTTTTGGCTGGAAAATACCACAGCTACCCTGAATCTTGGATACCATCTTCCATGGATAGCTTTGAGCTTTCTCTTCAGTTTGGAATTTCTGTCATGGTTATAGCATGCCCTTGTGCTTTAGGCCTAGCAACTCCCACAGCTGTCATGGTTGGAACTGGAGTAGGCGCATTTCAAGGTGTATTGATCAAAGGAGGCCAAGCATTAGAAAGTGCACATAAGGTGAACTGCATTGTGTTCGACAAGACGGGGACTCTAACAATTGGAAAGCCAGTGGTTGTCAACACGCGACTTTTGAAAAATATGGTGCTTCAAGAATTCTATGAACTTGTTGCTGCAGCTGAGGTGAATAGCGAACACCCGTTGGCTAAGGCCATTGTTGAGTATGCCAAAAAGTTCAGAGAAGACGAAGAGAATCCTGCTTGGCCAGAAGCAAAACACTTTGAATCCATTACTGGGCATGGGGTGAAGGCCATTGTTAGGAACAAGGAAATAATTGTTGGCAACAAGAGCTTGATAGTGGAGCACAGCATTGCGGTTCCAATTGATGCAGAAGAGATACTTGCAAAAGCGGAAGGACTCGCTCAAACTGTAATTTTAATAGCTATAGATGGAGAAGTGGCTGGAGTTCTGTCCATATCTGATCCACTGAAACCAAGTGCTCGAGAAGTAATTTCCATACTCAAGTCTATGAAAGTTACAAGTATAATGGTGACAGGTGACAACTGGGGAACTGCAAATTCTATCGCTAAGGAAGTTGGAATTGAGACCATTATAGCAGAAGCCAAACCCGATCAGAAAGCAGAGAAAGTGAAGGATTTGCAGGCTTCAGGCTACACTGTGGCAATGGTGGGAGACGGAATCAATGACTCACCGGCACTTGTGGCAGCAGATGTAGGAATGGCAATTGGTGCAGGAACAGACATTGCCATTGAGGCAGCTGACATTGTTCTAGTGAAGAGCAACTTGGAAGATGTGATAACTGCGATTCACCTTTCTAGGAAAACCTTCACCCGTATCCGTTTGAACTACATCTGGGCTTTGGGATATAACGTTCTTGGCATTCCAATAGCTGCAGGAGCCCTATTCCCACCTACTGGATTTCGTTTACCACCATGGATTGCTGGAGCTGCAATGGCAGCTTCTTCTGTCAGTGTTGTTTGCTGCTCTCTGCTGTTGAAGAATTATAAGAAGCCAAAGGTTCTGGACAACTTGGAGGTACGCGGAATAAGTATTGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0008150 GO:0008324 GO:0009636 GO:0010035 GO:0010038 GO:0010273 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042221 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046688 GO:0046873 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0061687 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0097501 GO:0098655 GO:0098660 GO:0098662 GO:0098754 GO:0099131 GO:0099132 GO:1990169

KEGG Pathways

Metabolic & Signaling

ko01524 ko04016 map01524 map04016

Pfam Domains

Protein Families

E1-E2_ATPase HMA Hydrolase

Protein Analysis

1002

Amino Acids

108.8

Weight (kDa)

6.04

Isoelectric Point (pI)

32.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMA	PF00403	51 - 110	6.6e-13	Heavy-metal-associated domain
HMA	PF00403	130 - 190	1.9e-15	Heavy-metal-associated domain
E1-E2_ATPase	PF00122	452 - 540	3.1e-23	P-type ATPase actuator domain
Hydrolase	PF00702	664 - 886	5.6e-33	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0002025)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G63440
fragaria_vesca	FvH4_5g02090 FvH4_5g02090 FvH4_5g02091 FvH4_5g02091 FvH4_5g02091 FvH4_5g02091
malus_domestica	MD06G1080800.v1.1 MD06G1080900.v1.1 MD14G1102600.v1.1
prunus_persica	Prupe.5G097900_v2.0.a1 Prupe.5G097900_v2.0.a1 Prupe.5G098000_v2.0.a1
pyrus_communis	pycom06g07780 pycom06g07790 pycom14g09890
rosa_chinensis	RchiOBHm_Chr7g0197781 RchiOBHm_Chr7g0198111 RchiOBHm_Chr7g0198141
rosa_laevigata	RLG00000003885 RLG00000003888 RLG00000019170
rosa_multiflora	Rmu_sc0000221.1_g000018 Rmu_sc0000221.1_g000030 Rmu_sc0006732.1_g000016
rosa_roxburghii	Rroxscaffold_3G00257940 Rroxscaffold_3G00257970 Rroxscaffold_3G00257990
rosa_rugosa	Rorug07G0045700 Rorug07G0045700 Rorug07G0045800 Rorug07G0045800 Rorug07G0045900
rosa_samantha	Rh7AG169100 Rh7AG169200 Rh7BG169900 Rh7BG172300 Rh7CG178600 Rh7CG178700 Rh7DG169200 Rh7DG171400 Rh7DG171800
rosa_wichuraiana	Rw7G014570 Rw7G014780 Rw7G014790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2952
AarI	CACCTGC	1 cut(s)	1720
AatII	GACGTC	1 cut(s)	246
Acc36I	ACCTGC	1 cut(s)	1720
AccB1I	GGYRCC	1 cut(s)	1303
AccB7I	CCANNNNNTGG	1 cut(s)	2861
AccI	GTMKAC	3 cut(s)	240, 696, 1146
AccII	CGCG	5 cut(s)	228, 293, 306, 2053, 2988
AciI	CCGC	9 cut(s)	104, 177, 293, 1089, 1316, 1663, 2300, 2336, 2988
AclI	AACGTT	1 cut(s)	2817
AclWI	GGATC	3 cut(s)	506, 1729, 2402
AcoI	YGGCCR	3 cut(s)	1017, 1632, 2189
AcsI	RAATTY	4 cut(s)	387, 1851, 2081, 2503
AcuI	CTGAAG	5 cut(s)	129, 510, 1413, 1826, 2577
AcyI	GRCGYC	1 cut(s)	243
AfaI	GTAC	7 cut(s)	89, 462, 1244, 1534, 1608, 1647, 2985
AfiI	CCNNNNNNNGG	6 cut(s)	186, 210, 792, 2220, 2244, 2861
AflII	CTTAAG	1 cut(s)	1708
AhlI	ACTAGT	1 cut(s)	1205
AjiI	CACGTC	2 cut(s)	171, 1715
AjnI	CCWGG	8 cut(s)	120, 679, 791, 817, 943, 1411, 1754, 1763
AjuI	GAANNNNNNNTTGG	4 cut(s)	2247, 2279, 2714, 2746
AleI	CACNNNNGTG	1 cut(s)	1025
AloI	GAACNNNNNNTCC	4 cut(s)	625, 657, 2802, 2834
Alw21I	GWGCWC	5 cut(s)	176, 1002, 1981, 2292, 2428
Alw26I	GTCTC	7 cut(s)	107, 290, 302, 669, 1196, 2525, 2613
Alw44I	GTGCAC	1 cut(s)	1977
AlwI	GGATC	3 cut(s)	506, 1729, 2402
Ama87I	CYCGRG	2 cut(s)	217, 2427
ApaLI	GTGCAC	1 cut(s)	1977
ApoI	RAATTY	4 cut(s)	387, 1851, 2081, 2503
ArsI	GACNNNNNNTTYG	2 cut(s)	548, 580
AseI	ATTAAT	2 cut(s)	345, 768
Asp700I	GAANNNNTTC	1 cut(s)	2771
AspLEI	GCGC	4 cut(s)	185, 262, 1080, 1934
AspS9I	GGNCC	5 cut(s)	19, 63, 633, 752, 789
AsuC2I	CCSGG	7 cut(s)	61, 187, 218, 219, 257, 787, 1025
AsuII	TTCGAA	1 cut(s)	567
AvaI	CYCGRG	2 cut(s)	217, 2427
AvaII	GGWCC	2 cut(s)	633, 789
BaeGI	GKGCMC	1 cut(s)	1981
BalI	TGGCCA	3 cut(s)	1019, 1634, 2191
BanI	GGYRCC	1 cut(s)	1303
BanII	GRGCYC	2 cut(s)	1002, 2848
BbsI	GAAGAC	4 cut(s)	561, 922, 1274, 2175
Bbv12I	GWGCWC	5 cut(s)	176, 1002, 1981, 2292, 2428
BbvCI	CCTCAGC	2 cut(s)	135, 2106
BccI	CCATC	8 cut(s)	449, 617, 1002, 1288, 1427, 1624, 1819, 2369
BceAI	ACGGC	2 cut(s)	188, 1625
BcgI	CGANNNNNNTGC	4 cut(s)	1190, 1224, 1984, 2018
BciT130I	CCWGG	8 cut(s)	122, 681, 793, 819, 945, 1413, 1756, 1765
BciVI	GTATCC	4 cut(s)	645, 1684, 1800, 2792
BclI	TGATCA	1 cut(s)	1950
BcnI	CCSGG	7 cut(s)	61, 187, 218, 219, 257, 787, 1025
BcoDI	GTCTC	7 cut(s)	107, 290, 302, 669, 1196, 2525, 2613
BcuI	ACTAGT	1 cut(s)	1205
BfaI	CTAG	8 cut(s)	962, 1206, 1247, 1331, 1572, 1892, 2717, 2763
BfmI	CTRYAG	5 cut(s)	585, 693, 2100, 2370, 2838
BfrI	CTTAAG	1 cut(s)	1708
BfuAI	ACCTGC	1 cut(s)	1720
BfuI	GTATCC	4 cut(s)	645, 1684, 1800, 2792
BglI	GCCNNNNNGGC	1 cut(s)	2699
Bme18I	GGWCC	2 cut(s)	633, 789
BmeT110I	CYCGRG	2 cut(s)	217, 2427
BmgBI	CACGTC	2 cut(s)	171, 1715
BmgT120I	GGNCC	5 cut(s)	19, 63, 633, 752, 789
BmiI	GGNNCC	7 cut(s)	65, 215, 790, 1087, 1305, 2304, 2845
BmrI	ACTGGG	3 cut(s)	1068, 2229, 2500
BmsI	GCATC	2 cut(s)	1267, 2302
BmuI	ACTGGG	3 cut(s)	1068, 2229, 2500
BpiI	GAAGAC	4 cut(s)	561, 922, 1274, 2175
BpmI	CTGGAG	4 cut(s)	1158, 1944, 2409, 2910
Bpu10I	CCTNAGC	5 cut(s)	135, 481, 2106, 2133, 2514
Bpu14I	TTCGAA	1 cut(s)	567
BpuEI	CTTGAG	1 cut(s)	2432
BpuMI	CCSGG	7 cut(s)	61, 187, 218, 219, 257, 787, 1025
BsaHI	GRCGYC	1 cut(s)	243
BsaI	GGTCTC	2 cut(s)	107, 2525
BsaWI	WCCGGW	1 cut(s)	907
BsaXI	ACNNNNNCTCC	2 cut(s)	59, 89
Bsc4I	CCNNNNNNNGG	6 cut(s)	186, 210, 792, 2220, 2244, 2861
Bse118I	RCCGGY	1 cut(s)	2638
Bse1I	ACTGG	9 cut(s)	565, 1063, 1496, 1692, 1927, 2036, 2224, 2495, 2866
Bse3DI	GCAATG	5 cut(s)	1281, 2295, 2616, 2688, 2904
BseBI	CCWGG	8 cut(s)	122, 681, 793, 819, 945, 1413, 1756, 1765
BseGI	GGATG	1 cut(s)	1105
BseLI	CCNNNNNNNGG	6 cut(s)	186, 210, 792, 2220, 2244, 2861
BseMI	GCAATG	5 cut(s)	1281, 2295, 2616, 2688, 2904
BseMII	CTCAG	3 cut(s)	126, 1640, 2097
BseNI	ACTGG	9 cut(s)	565, 1063, 1496, 1692, 1927, 2036, 2224, 2495, 2866
BseRI	GAGGAG	1 cut(s)	368
BseSI	GKGCMC	1 cut(s)	1981
BseYI	CCCAGC	1 cut(s)	42
BsgI	GTGCAG	1 cut(s)	2697
Bsh1236I	CGCG	5 cut(s)	228, 293, 306, 2053, 2988
Bsh1285I	CGRYCG	1 cut(s)	640
BshNI	GGYRCC	1 cut(s)	1303
BsiEI	CGRYCG	1 cut(s)	640
BsiHKAI	GWGCWC	5 cut(s)	176, 1002, 1981, 2292, 2428
BsiHKCI	CYCGRG	2 cut(s)	217, 2427
BslFI	GGGAC	2 cut(s)	1201, 2029
BslI	CCNNNNNNNGG	6 cut(s)	186, 210, 792, 2220, 2244, 2861
BsmAI	GTCTC	7 cut(s)	107, 290, 302, 669, 1196, 2525, 2613
BsmBI	CGTCTC	2 cut(s)	302, 2613
BsmFI	GGGAC	2 cut(s)	1201, 2029
BsmI	GAATGC	1 cut(s)	2826
Bso31I	GGTCTC	2 cut(s)	107, 2525
BsoBI	CYCGRG	2 cut(s)	217, 2427
Bsp119I	TTCGAA	1 cut(s)	567
Bsp1286I	GDGCHC	6 cut(s)	176, 1002, 1981, 2292, 2428, 2848
Bsp1407I	TGTACA	2 cut(s)	460, 1645
Bsp143I	GATC	6 cut(s)	511, 637, 1734, 1950, 2407, 2557
Bsp19I	CCATGG	4 cut(s)	924, 1081, 1818, 2879
Bsp68I	TCGCGA	2 cut(s)	228, 306
BspACI	CCGC	9 cut(s)	104, 177, 293, 1089, 1316, 1663, 2300, 2336, 2988
BspCNI	CTCAG	3 cut(s)	127, 1639, 2098
BspFNI	CGCG	5 cut(s)	228, 293, 306, 2053, 2988
BspHI	TCATGA	2 cut(s)	987, 1002
BspLI	GGNNCC	7 cut(s)	65, 215, 790, 1087, 1305, 2304, 2845
BspMAI	CTGCAG	3 cut(s)	589, 2104, 2842
BspMI	ACCTGC	1 cut(s)	1720
BspPI	GGATC	3 cut(s)	506, 1729, 2402
BspQI	GCTCTTC	1 cut(s)	2717
BspT104I	TTCGAA	1 cut(s)	567
BspT107I	GGYRCC	1 cut(s)	1303
BspTI	CTTAAG	1 cut(s)	1708
BspTNI	GGTCTC	2 cut(s)	107, 2525
BsrDI	GCAATG	5 cut(s)	1281, 2295, 2616, 2688, 2904
BsrFI	RCCGGY	1 cut(s)	2638
BsrGI	TGTACA	2 cut(s)	460, 1645
BsrI	ACTGG	9 cut(s)	565, 1063, 1496, 1692, 1927, 2036, 2224, 2495, 2866
BssAI	RCCGGY	1 cut(s)	2638
BssMI	GATC	6 cut(s)	511, 637, 1734, 1950, 2407, 2557
BssNI	GRCGYC	1 cut(s)	243
BssT1I	CCWWGG	5 cut(s)	924, 1081, 1234, 1818, 2879
Bst2UI	CCWGG	8 cut(s)	122, 681, 793, 819, 945, 1413, 1756, 1765
Bst4CI	ACNGT	4 cut(s)	430, 663, 2356, 2605
Bst6I	CTCTTC	7 cut(s)	105, 672, 829, 1845, 2169, 2313, 2717
BstACI	GRCGYC	1 cut(s)	243
BstAFI	CTTAAG	1 cut(s)	1708
BstAPI	GCANNNNNTGC	3 cut(s)	171, 417, 2675
BstAUI	TGTACA	2 cut(s)	460, 1645
BstBI	TTCGAA	1 cut(s)	567
BstC8I	GCNNGC	4 cut(s)	181, 690, 1873, 2589
BstDEI	CTNAG	7 cut(s)	135, 481, 1260, 1626, 2106, 2133, 2514
BstDSI	CCRYGG	4 cut(s)	924, 1081, 1818, 2879
BstEII	GGTNACC	1 cut(s)	47
BstF5I	GGATG	1 cut(s)	1105
BstFNI	CGCG	5 cut(s)	228, 293, 306, 2053, 2988
BstHHI	GCGC	4 cut(s)	185, 262, 1080, 1934
BstKTI	GATC	6 cut(s)	514, 640, 1737, 1953, 2410, 2560
BstMAI	GTCTC	7 cut(s)	107, 290, 302, 669, 1196, 2525, 2613
BstMBI	GATC	6 cut(s)	511, 637, 1734, 1950, 2407, 2557
BstMCI	CGRYCG	1 cut(s)	640
BstNI	CCWGG	8 cut(s)	122, 681, 793, 819, 945, 1413, 1756, 1765
BstNSI	RCATGY	1 cut(s)	1875
BstPI	GGTNACC	1 cut(s)	47
BstSFI	CTRYAG	5 cut(s)	585, 693, 2100, 2370, 2838
BstSLI	GKGCMC	1 cut(s)	1981
BstUI	CGCG	5 cut(s)	228, 293, 306, 2053, 2988
BstV2I	GAAGAC	4 cut(s)	561, 922, 1274, 2175
BstXI	CCANNNNNNTGG	3 cut(s)	16, 100, 1294
BsuI	GTATCC	4 cut(s)	645, 1684, 1800, 2792
BtgI	CCRYGG	4 cut(s)	924, 1081, 1818, 2879
BtrI	CACGTC	2 cut(s)	171, 1715
BtsCI	GGATG	1 cut(s)	1105
BtsI	GCAGTG	1 cut(s)	1073
BtsIMutI	CAGTG	8 cut(s)	426, 558, 1073, 1685, 2043, 2411, 2601, 2923
BtuMI	TCGCGA	2 cut(s)	228, 306
BveI	ACCTGC	1 cut(s)	1720
Cac8I	GCNNGC	4 cut(s)	181, 690, 1873, 2589
CciI	TCATGA	2 cut(s)	987, 1002
CfoI	GCGC	4 cut(s)	185, 262, 1080, 1934
Cfr10I	RCCGGY	1 cut(s)	2638
Cfr13I	GGNCC	5 cut(s)	19, 63, 633, 752, 789
Cfr9I	CCCGGG	1 cut(s)	217
Csp6I	GTAC	7 cut(s)	88, 461, 1243, 1533, 1607, 1646, 2984
CviQI	GTAC	7 cut(s)	88, 461, 1243, 1533, 1607, 1646, 2984
DdeI	CTNAG	7 cut(s)	135, 481, 1260, 1626, 2106, 2133, 2514
DpnI	GATC	6 cut(s)	513, 639, 1736, 1952, 2409, 2559
DpnII	GATC	6 cut(s)	511, 637, 1734, 1950, 2407, 2557
EaeI	YGGCCR	3 cut(s)	1017, 1632, 2189
Eam1104I	CTCTTC	7 cut(s)	105, 672, 829, 1845, 2169, 2313, 2717
EarI	CTCTTC	7 cut(s)	105, 672, 829, 1845, 2169, 2313, 2717
EciI	GGCGGA	2 cut(s)	166, 1078
Ecl136II	GAGCTC	1 cut(s)	1000
Eco130I	CCWWGG	5 cut(s)	924, 1081, 1234, 1818, 2879
Eco147I	AGGCCT	1 cut(s)	1890
Eco24I	GRGCYC	2 cut(s)	1002, 2848
Eco31I	GGTCTC	2 cut(s)	107, 2525
Eco47I	GGWCC	2 cut(s)	633, 789
Eco53kI	GAGCTC	1 cut(s)	1000
Eco57I	CTGAAG	5 cut(s)	129, 510, 1413, 1826, 2577
Eco88I	CYCGRG	2 cut(s)	217, 2427
Eco91I	GGTNACC	1 cut(s)	47
EcoICRI	GAGCTC	1 cut(s)	1000
EcoO65I	GGTNACC	1 cut(s)	47
EcoRI	GAATTC	2 cut(s)	387, 2081
EcoRII	CCWGG	8 cut(s)	120, 679, 791, 817, 943, 1411, 1754, 1763
EcoT14I	CCWWGG	5 cut(s)	924, 1081, 1234, 1818, 2879
EcoT22I	ATGCAT	1 cut(s)	29
EcoT38I	GRGCYC	2 cut(s)	1002, 2848
ErhI	CCWWGG	5 cut(s)	924, 1081, 1234, 1818, 2879
Esp3I	CGTCTC	2 cut(s)	302, 2613
FalI	AAGNNNNNCTT	4 cut(s)	2310, 2342, 2714, 2746
FaqI	GGGAC	2 cut(s)	1201, 2029
FbaI	TGATCA	1 cut(s)	1950
FblI	GTMKAC	3 cut(s)	240, 696, 1146
FokI	GGATG	1 cut(s)	1112
FriOI	GRGCYC	2 cut(s)	1002, 2848
FspBI	CTAG	8 cut(s)	962, 1206, 1247, 1331, 1572, 1892, 2717, 2763
GlaI	GCGC	4 cut(s)	184, 261, 1079, 1933
GsaI	CCCAGC	1 cut(s)	46
GsuI	CTGGAG	4 cut(s)	1158, 1944, 2409, 2910
HhaI	GCGC	4 cut(s)	185, 262, 1080, 1934
Hin1I	GRCGYC	1 cut(s)	243
Hin6I	GCGC	4 cut(s)	183, 260, 1078, 1932
HinP1I	GCGC	4 cut(s)	183, 260, 1078, 1932
HincII	GTYRAC	3 cut(s)	241, 289, 2047
HindII	GTYRAC	3 cut(s)	241, 289, 2047
HpaI	GTTAAC	1 cut(s)	289
Hpy99I	CGWCG	1 cut(s)	245
HpyAV	CCTTC	6 cut(s)	193, 563, 2227, 2333, 2572, 2782
HpyCH4III	ACNGT	4 cut(s)	430, 663, 2356, 2605
HpyCH4IV	ACGT	5 cut(s)	170, 243, 285, 1714, 2817
HpyF3I	CTNAG	7 cut(s)	135, 481, 1260, 1626, 2106, 2133, 2514
HpySE526I	ACGT	5 cut(s)	170, 243, 285, 1714, 2817
Hsp92I	GRCGYC	1 cut(s)	243
HspAI	GCGC	4 cut(s)	183, 260, 1078, 1932
Ksp22I	TGATCA	1 cut(s)	1950
KspAI	GTTAAC	1 cut(s)	289
Kzo9I	GATC	6 cut(s)	511, 637, 1734, 1950, 2407, 2557
LguI	GCTCTTC	1 cut(s)	2717
LmnI	GCTCC	8 cut(s)	131, 179, 219, 997, 1645, 2287, 2843, 2891
LweI	GCATC	2 cut(s)	1267, 2302
MaeI	CTAG	8 cut(s)	962, 1206, 1247, 1331, 1572, 1892, 2717, 2763
MaeII	ACGT	5 cut(s)	170, 243, 285, 1714, 2817
MaeIII	GTNAC	7 cut(s)	47, 157, 1441, 1514, 2461, 2476, 2483
MalI	GATC	6 cut(s)	513, 639, 1736, 1952, 2409, 2559
MboI	GATC	6 cut(s)	511, 637, 1734, 1950, 2407, 2557
MfeI	CAATTG	3 cut(s)	2025, 2307, 2670
MhlI	GDGCHC	6 cut(s)	176, 1002, 1981, 2292, 2428, 2848
MlsI	TGGCCA	3 cut(s)	1019, 1634, 2191
MluNI	TGGCCA	3 cut(s)	1019, 1634, 2191
MlyI	GAGTC	6 cut(s)	885, 1511, 1628, 2011, 2337, 2627
MmeI	TCCRAC	3 cut(s)	304, 1897, 2503
Mox20I	TGGCCA	3 cut(s)	1019, 1634, 2191
Mph1103I	ATGCAT	1 cut(s)	29
MroXI	GAANNNNTTC	1 cut(s)	2771
MscI	TGGCCA	3 cut(s)	1019, 1634, 2191
MslI	CAYNNNNRTG	5 cut(s)	164, 379, 770, 1025, 1464
Msp20I	TGGCCA	3 cut(s)	1019, 1634, 2191
MspA1I	CMGCKG	5 cut(s)	538, 1316, 1907, 2105, 2705
MspCI	CTTAAG	1 cut(s)	1708
MunI	CAATTG	3 cut(s)	2025, 2307, 2670
Mva1269I	GAATGC	1 cut(s)	2826
MvaI	CCWGG	8 cut(s)	122, 681, 793, 819, 945, 1413, 1756, 1765
MvnI	CGCG	5 cut(s)	228, 293, 306, 2053, 2988
NciI	CCSGG	7 cut(s)	61, 187, 218, 219, 257, 787, 1025
NcoI	CCATGG	4 cut(s)	924, 1081, 1818, 2879
NdeII	GATC	6 cut(s)	511, 637, 1734, 1950, 2407, 2557
NlaIV	GGNNCC	7 cut(s)	65, 215, 790, 1087, 1305, 2304, 2845
NmuCI	GTSAC	5 cut(s)	47, 157, 1514, 2476, 2483
NruI	TCGCGA	2 cut(s)	228, 306
NsiI	ATGCAT	1 cut(s)	29
NspI	RCATGY	1 cut(s)	1875
NspV	TTCGAA	1 cut(s)	567
OliI	CACNNNNGTG	1 cut(s)	1025
PaeI	GCATGC	1 cut(s)	1875
PaeR7I	CTCGAG	1 cut(s)	2427
PagI	TCATGA	2 cut(s)	987, 1002
PaqCI	CACCTGC	1 cut(s)	1720
PceI	AGGCCT	1 cut(s)	1890
PciSI	GCTCTTC	1 cut(s)	2717
PcsI	WCGNNNNNNNCGW	2 cut(s)	167, 201
PctI	GAATGC	1 cut(s)	2826
PdmI	GAANNNNTTC	1 cut(s)	2771
PflFI	GACNNNGTC	1 cut(s)	1520
PflMI	CCANNNNNTGG	1 cut(s)	2861
PfoI	TCCNGGA	2 cut(s)	817, 943
Ple19I	CGATCG	1 cut(s)	640
PleI	GAGTC	6 cut(s)	884, 1511, 1627, 2011, 2337, 2627
PpsI	GAGTC	6 cut(s)	884, 1511, 1627, 2011, 2337, 2627
PshBI	ATTAAT	2 cut(s)	345, 768
PsiI	TTATAA	1 cut(s)	2952
Psp124BI	GAGCTC	1 cut(s)	1002
Psp1406I	AACGTT	1 cut(s)	2817
Psp6I	CCWGG	8 cut(s)	120, 679, 791, 817, 943, 1411, 1754, 1763
PspEI	GGTNACC	1 cut(s)	47
PspFI	CCCAGC	1 cut(s)	42
PspGI	CCWGG	8 cut(s)	120, 679, 791, 817, 943, 1411, 1754, 1763
PspN4I	GGNNCC	7 cut(s)	65, 215, 790, 1087, 1305, 2304, 2845
PspPI	GGNCC	5 cut(s)	19, 63, 633, 752, 789
PstI	CTGCAG	3 cut(s)	589, 2104, 2842
PsyI	GACNNNGTC	1 cut(s)	1520
PvuI	CGATCG	1 cut(s)	640
PvuII	CAGCTG	4 cut(s)	538, 1907, 2105, 2705
RruI	TCGCGA	2 cut(s)	228, 306
RsaI	GTAC	7 cut(s)	89, 462, 1244, 1534, 1608, 1647, 2985
RsaNI	GTAC	7 cut(s)	88, 461, 1243, 1533, 1607, 1646, 2984
RseI	CAYNNNNRTG	5 cut(s)	164, 379, 770, 1025, 1464
SacI	GAGCTC	1 cut(s)	1002
SalI	GTCGAC	1 cut(s)	239
SapI	GCTCTTC	1 cut(s)	2717
Sau3AI	GATC	6 cut(s)	511, 637, 1734, 1950, 2407, 2557
Sau96I	GGNCC	5 cut(s)	19, 63, 633, 752, 789
SchI	GAGTC	6 cut(s)	885, 1511, 1628, 2011, 2337, 2627
SduI	GDGCHC	6 cut(s)	176, 1002, 1981, 2292, 2428, 2848
SfaNI	GCATC	2 cut(s)	1267, 2302
SfcI	CTRYAG	5 cut(s)	585, 693, 2100, 2370, 2838
Sfr274I	CTCGAG	1 cut(s)	2427
SfuI	TTCGAA	1 cut(s)	567
SinI	GGWCC	2 cut(s)	633, 789
SlaI	CTCGAG	1 cut(s)	2427
SmaI	CCCGGG	1 cut(s)	219
SmiMI	CAYNNNNRTG	5 cut(s)	164, 379, 770, 1025, 1464
SmlI	CTYRAG	3 cut(s)	1708, 2427, 2447
SmoI	CTYRAG	3 cut(s)	1708, 2427, 2447
SpeI	ACTAGT	1 cut(s)	1205
SphI	GCATGC	1 cut(s)	1875
SseBI	AGGCCT	1 cut(s)	1890
SsiI	CCGC	9 cut(s)	104, 177, 293, 1089, 1316, 1663, 2300, 2336, 2988
SspMI	CTAG	8 cut(s)	962, 1206, 1247, 1331, 1572, 1892, 2717, 2763
SstI	GAGCTC	1 cut(s)	1002
StuI	AGGCCT	1 cut(s)	1890
StyI	CCWWGG	5 cut(s)	924, 1081, 1234, 1818, 2879
TaaI	ACNGT	4 cut(s)	430, 663, 2356, 2605
TaiI	ACGT	5 cut(s)	173, 246, 288, 1717, 2820
TaqI	TCGA	8 cut(s)	195, 240, 422, 567, 702, 1200, 2004, 2428
TaqII	GACCGA	3 cut(s)	650, 1132, 1510
TatI	WGTACW	2 cut(s)	460, 1645
TauI	GCSGC	2 cut(s)	107, 1319
TscAI	CASTG	8 cut(s)	433, 565, 1080, 1692, 2043, 2418, 2608, 2923
TseFI	GTSAC	5 cut(s)	47, 157, 1514, 2476, 2483
Tsp45I	GTSAC	5 cut(s)	47, 157, 1514, 2476, 2483
TspGWI	ACGGA	2 cut(s)	2637, 2774
TspMI	CCCGGG	1 cut(s)	217
TspRI	CASTG	8 cut(s)	433, 565, 1080, 1692, 2043, 2418, 2608, 2923
Tth111I	GACNNNGTC	1 cut(s)	1520
Van91I	CCANNNNNTGG	1 cut(s)	2861
Vha464I	CTTAAG	1 cut(s)	1708
VneI	GTGCAC	1 cut(s)	1977
VpaK11BI	GGWCC	2 cut(s)	633, 789
VspI	ATTAAT	2 cut(s)	345, 768
XapI	RAATTY	4 cut(s)	387, 1851, 2081, 2503
XceI	RCATGY	1 cut(s)	1875
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1909
XhoI	CTCGAG	1 cut(s)	2427
XmaI	CCCGGG	1 cut(s)	217
XmiI	GTMKAC	3 cut(s)	240, 696, 1146
XmnI	GAANNNNTTC	1 cut(s)	2771
XspI	CTAG	8 cut(s)	962, 1206, 1247, 1331, 1572, 1892, 2717, 2763
ZraI	GACGTC	1 cut(s)	244
Zsp2I	ATGCAT	1 cut(s)	29

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.