Belongs to the class-I aminoacyl-tRNA synthetase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 1554 |
| Acc36I | ACCTGC | 1 cut(s) | 1554 |
| AccB1I | GGYRCC | 2 cut(s) | 367, 1325 |
| AccII | CGCG | 1 cut(s) | 1263 |
| AciI | CCGC | 5 cut(s) | 213, 727, 1204, 1263, 1726 |
| AclWI | GGATC | 4 cut(s) | 710, 1242, 1451, 1521 |
| AcoI | YGGCCR | 2 cut(s) | 1045, 1674 |
| AcsI | RAATTY | 5 cut(s) | 26, 131, 575, 650, 1643 |
| AcuI | CTGAAG | 5 cut(s) | 605, 689, 1352, 1367, 1535 |
| AcyI | GRCGYC | 1 cut(s) | 46 |
| AfaI | GTAC | 4 cut(s) | 193, 303, 491, 1613 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 218, 706, 736, 1440 |
| AflII | CTTAAG | 1 cut(s) | 77 |
| AgsI | TTSAA | 8 cut(s) | 335, 503, 649, 661, 806, 881, 914, 1075 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1689 |
| AjnI | CCWGG | 3 cut(s) | 124, 283, 729 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 420, 452 |
| Alw21I | GWGCWC | 2 cut(s) | 898, 1583 |
| Alw26I | GTCTC | 6 cut(s) | 177, 245, 596, 1556, 1676, 1697 |
| Alw44I | GTGCAC | 1 cut(s) | 1579 |
| AlwI | GGATC | 4 cut(s) | 710, 1242, 1451, 1521 |
| Ama87I | CYCGRG | 3 cut(s) | 485, 1185, 1286 |
| AoxI | GGCC | 3 cut(s) | 1045, 1227, 1674 |
| ApaLI | GTGCAC | 1 cut(s) | 1579 |
| ApeKI | GCWGC | 7 cut(s) | 864, 1051, 1054, 1079, 1190, 1247, 1520 |
| ApoI | RAATTY | 5 cut(s) | 26, 131, 575, 650, 1643 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 507, 1733 |
| AspLEI | GCGC | 1 cut(s) | 1263 |
| AspS9I | GGNCC | 4 cut(s) | 206, 281, 697, 1297 |
| AsuHPI | GGTGA | 8 cut(s) | 22, 476, 539, 1275, 1535, 1630, 1675, 1744 |
| AsuII | TTCGAA | 1 cut(s) | 793 |
| AvaI | CYCGRG | 3 cut(s) | 485, 1185, 1286 |
| AvaII | GGWCC | 4 cut(s) | 206, 281, 697, 1297 |
| BaeGI | GKGCMC | 4 cut(s) | 370, 1330, 1534, 1583 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1401, 1434 |
| BalI | TGGCCA | 1 cut(s) | 1047 |
| BanI | GGYRCC | 2 cut(s) | 367, 1325 |
| BanII | GRGCYC | 1 cut(s) | 114 |
| BauI | CACGAG | 1 cut(s) | 897 |
| BbsI | GAAGAC | 2 cut(s) | 695, 1355 |
| Bbv12I | GWGCWC | 2 cut(s) | 898, 1583 |
| BbvCI | CCTCAGC | 1 cut(s) | 1308 |
| BbvI | GCAGC | 7 cut(s) | 876, 1063, 1066, 1066, 1202, 1234, 1532 |
| BccI | CCATC | 5 cut(s) | 250, 313, 344, 944, 1089 |
| BceAI | ACGGC | 3 cut(s) | 1400, 1674, 1728 |
| BciT130I | CCWGG | 3 cut(s) | 126, 285, 731 |
| BclI | TGATCA | 1 cut(s) | 1384 |
| BcoDI | GTCTC | 6 cut(s) | 177, 245, 596, 1556, 1676, 1697 |
| BfaI | CTAG | 6 cut(s) | 18, 473, 1125, 1208, 1220, 1535 |
| BfmI | CTRYAG | 3 cut(s) | 273, 393, 1716 |
| BfrI | CTTAAG | 1 cut(s) | 77 |
| BfuAI | ACCTGC | 1 cut(s) | 1554 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1529 |
| BglII | AGATCT | 1 cut(s) | 616 |
| BisI | GCNGC | 7 cut(s) | 865, 1052, 1055, 1080, 1191, 1248, 1521 |
| BlpI | GCTNAGC | 1 cut(s) | 57 |
| BlsI | GCNGC | 7 cut(s) | 866, 1053, 1056, 1081, 1192, 1249, 1522 |
| BmcAI | AGTACT | 1 cut(s) | 491 |
| Bme1390I | CCNGG | 3 cut(s) | 126, 285, 731 |
| Bme18I | GGWCC | 4 cut(s) | 206, 281, 697, 1297 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1689 |
| BmeT110I | CYCGRG | 3 cut(s) | 485, 1185, 1286 |
| BmgT120I | GGNCC | 4 cut(s) | 206, 281, 697, 1297 |
| BmiI | GGNNCC | 3 cut(s) | 90, 369, 1327 |
| BmrFI | CCNGG | 3 cut(s) | 126, 285, 731 |
| BmrI | ACTGGG | 1 cut(s) | 541 |
| BmsI | GCATC | 1 cut(s) | 540 |
| BmuI | ACTGGG | 1 cut(s) | 541 |
| BoxI | GACNNNNGTC | 1 cut(s) | 169 |
| BpiI | GAAGAC | 2 cut(s) | 695, 1355 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1308 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 57 |
| Bpu14I | TTCGAA | 1 cut(s) | 793 |
| BsaBI | GATNNNNATC | 1 cut(s) | 660 |
| BsaHI | GRCGYC | 1 cut(s) | 46 |
| BsaI | GGTCTC | 1 cut(s) | 177 |
| BsaJI | CCNNGG | 6 cut(s) | 202, 774, 1156, 1230, 1287, 1677 |
| BsaWI | WCCGGW | 1 cut(s) | 32 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 705, 735, 1562, 1592 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 218, 706, 736, 1440 |
| Bse1I | ACTGG | 1 cut(s) | 536 |
| Bse3DI | GCAATG | 2 cut(s) | 165, 417 |
| Bse8I | GATNNNNATC | 1 cut(s) | 660 |
| BseBI | CCWGG | 3 cut(s) | 126, 285, 731 |
| BseDI | CCNNGG | 6 cut(s) | 202, 774, 1156, 1230, 1287, 1677 |
| BseGI | GGATG | 3 cut(s) | 324, 555, 1100 |
| BseJI | GATNNNNATC | 1 cut(s) | 660 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 218, 706, 736, 1440 |
| BseMI | GCAATG | 2 cut(s) | 165, 417 |
| BseMII | CTCAG | 5 cut(s) | 243, 657, 1008, 1299, 1551 |
| BseNI | ACTGG | 1 cut(s) | 536 |
| BseRI | GAGGAG | 2 cut(s) | 1304, 1694 |
| BseSI | GKGCMC | 4 cut(s) | 370, 1330, 1534, 1583 |
| BseX3I | CGGCCG | 1 cut(s) | 1674 |
| BseXI | GCAGC | 7 cut(s) | 876, 1063, 1066, 1066, 1202, 1234, 1532 |
| Bsh1236I | CGCG | 1 cut(s) | 1263 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1677 |
| BshFI | GGCC | 3 cut(s) | 1047, 1229, 1676 |
| BshNI | GGYRCC | 2 cut(s) | 367, 1325 |
| BsiEI | CGRYCG | 1 cut(s) | 1677 |
| BsiHKAI | GWGCWC | 2 cut(s) | 898, 1583 |
| BsiHKCI | CYCGRG | 3 cut(s) | 485, 1185, 1286 |
| BsiSI | CCGG | 1 cut(s) | 33 |
| BslFI | GGGAC | 1 cut(s) | 177 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 218, 706, 736, 1440 |
| BsmAI | GTCTC | 6 cut(s) | 177, 245, 596, 1556, 1676, 1697 |
| BsmBI | CGTCTC | 3 cut(s) | 1556, 1676, 1697 |
| BsmFI | GGGAC | 1 cut(s) | 177 |
| BsnI | GGCC | 3 cut(s) | 1047, 1229, 1676 |
| Bso31I | GGTCTC | 1 cut(s) | 177 |
| BsoBI | CYCGRG | 3 cut(s) | 485, 1185, 1286 |
| Bsp119I | TTCGAA | 1 cut(s) | 793 |
| Bsp1286I | GDGCHC | 6 cut(s) | 114, 370, 898, 1330, 1534, 1583 |
| Bsp143I | GATC | 9 cut(s) | 117, 449, 616, 715, 976, 1234, 1384, 1443, 1513 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 57 |
| BspACI | CCGC | 5 cut(s) | 213, 727, 1204, 1263, 1726 |
| BspANI | GGCC | 3 cut(s) | 1047, 1229, 1676 |
| BspCNI | CTCAG | 5 cut(s) | 244, 658, 1009, 1300, 1552 |
| BspFNI | CGCG | 1 cut(s) | 1263 |
| BspLI | GGNNCC | 3 cut(s) | 90, 369, 1327 |
| BspMI | ACCTGC | 1 cut(s) | 1554 |
| BspPI | GGATC | 4 cut(s) | 710, 1242, 1451, 1521 |
| BspQI | GCTCTTC | 1 cut(s) | 68 |
| BspT104I | TTCGAA | 1 cut(s) | 793 |
| BspT107I | GGYRCC | 2 cut(s) | 367, 1325 |
| BspTI | CTTAAG | 1 cut(s) | 77 |
| BspTNI | GGTCTC | 1 cut(s) | 177 |
| BsrDI | GCAATG | 2 cut(s) | 165, 417 |
| BsrI | ACTGG | 1 cut(s) | 536 |
| BssECI | CCNNGG | 6 cut(s) | 202, 774, 1156, 1230, 1287, 1677 |
| BssMI | GATC | 9 cut(s) | 117, 449, 616, 715, 976, 1234, 1384, 1443, 1513 |
| BssNI | GRCGYC | 1 cut(s) | 46 |
| BssSI | CACGAG | 1 cut(s) | 897 |
| BssT1I | CCWWGG | 3 cut(s) | 774, 1156, 1230 |
| Bst2BI | CACGAG | 1 cut(s) | 897 |
| Bst2UI | CCWGG | 3 cut(s) | 126, 285, 731 |
| Bst4CI | ACNGT | 3 cut(s) | 88, 543, 1567 |
| Bst6I | CTCTTC | 3 cut(s) | 68, 1002, 1626 |
| BstACI | GRCGYC | 1 cut(s) | 46 |
| BstAFI | CTTAAG | 1 cut(s) | 77 |
| BstBI | TTCGAA | 1 cut(s) | 793 |
| BstC8I | GCNNGC | 4 cut(s) | 478, 482, 1049, 1579 |
| BstDSI | CCRYGG | 1 cut(s) | 202 |
| BstEII | GGTNACC | 1 cut(s) | 1281 |
| BstF5I | GGATG | 3 cut(s) | 324, 555, 1100 |
| BstFNI | CGCG | 1 cut(s) | 1263 |
| BstHHI | GCGC | 1 cut(s) | 1263 |
| BstKTI | GATC | 9 cut(s) | 120, 452, 619, 718, 979, 1237, 1387, 1446, 1516 |
| BstMAI | GTCTC | 6 cut(s) | 177, 245, 596, 1556, 1676, 1697 |
| BstMBI | GATC | 9 cut(s) | 117, 449, 616, 715, 976, 1234, 1384, 1443, 1513 |
| BstMCI | CGRYCG | 1 cut(s) | 1677 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 689, 756, 1088, 1529, 1546 |
| BstNI | CCWGG | 3 cut(s) | 126, 285, 731 |
| BstNSI | RCATGY | 2 cut(s) | 437, 484 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 169 |
| BstPI | GGTNACC | 1 cut(s) | 1281 |
| BstSCI | CCNGG | 3 cut(s) | 124, 283, 729 |
| BstSFI | CTRYAG | 3 cut(s) | 273, 393, 1716 |
| BstSLI | GKGCMC | 4 cut(s) | 370, 1330, 1534, 1583 |
| BstUI | CGCG | 1 cut(s) | 1263 |
| BstV1I | GCAGC | 7 cut(s) | 876, 1063, 1066, 1066, 1202, 1234, 1532 |
| BstV2I | GAAGAC | 2 cut(s) | 695, 1355 |
| BstX2I | RGATCY | 3 cut(s) | 616, 715, 1443 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1745 |
| BstYI | RGATCY | 3 cut(s) | 616, 715, 1443 |
| BstZI | CGGCCG | 1 cut(s) | 1674 |
| BsuRI | GGCC | 3 cut(s) | 1047, 1229, 1676 |
| BtgI | CCRYGG | 1 cut(s) | 202 |
| BtsCI | GGATG | 3 cut(s) | 324, 555, 1100 |
| BveI | ACCTGC | 1 cut(s) | 1554 |
| Cac8I | GCNNGC | 4 cut(s) | 478, 482, 1049, 1579 |
| CfoI | GCGC | 1 cut(s) | 1263 |
| Cfr13I | GGNCC | 4 cut(s) | 206, 281, 697, 1297 |
| CseI | GACGC | 1 cut(s) | 54 |
| Csp6I | GTAC | 4 cut(s) | 192, 302, 490, 1612 |
| CviQI | GTAC | 4 cut(s) | 192, 302, 490, 1612 |
| DpnI | GATC | 9 cut(s) | 119, 451, 618, 717, 978, 1236, 1386, 1445, 1515 |
| DpnII | GATC | 9 cut(s) | 117, 449, 616, 715, 976, 1234, 1384, 1443, 1513 |
| DraI | TTTAAA | 1 cut(s) | 922 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1689 |
| EaeI | YGGCCR | 2 cut(s) | 1045, 1674 |
| EagI | CGGCCG | 1 cut(s) | 1674 |
| Eam1104I | CTCTTC | 3 cut(s) | 68, 1002, 1626 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1689 |
| EarI | CTCTTC | 3 cut(s) | 68, 1002, 1626 |
| EciI | GGCGGA | 1 cut(s) | 1193 |
| EclXI | CGGCCG | 1 cut(s) | 1674 |
| Eco130I | CCWWGG | 3 cut(s) | 774, 1156, 1230 |
| Eco24I | GRGCYC | 1 cut(s) | 114 |
| Eco31I | GGTCTC | 1 cut(s) | 177 |
| Eco47I | GGWCC | 4 cut(s) | 206, 281, 697, 1297 |
| Eco52I | CGGCCG | 1 cut(s) | 1674 |
| Eco57I | CTGAAG | 5 cut(s) | 605, 689, 1352, 1367, 1535 |
| Eco88I | CYCGRG | 3 cut(s) | 485, 1185, 1286 |
| Eco91I | GGTNACC | 1 cut(s) | 1281 |
| EcoO65I | GGTNACC | 1 cut(s) | 1281 |
| EcoRI | GAATTC | 1 cut(s) | 26 |
| EcoRII | CCWGG | 3 cut(s) | 124, 283, 729 |
| EcoT14I | CCWWGG | 3 cut(s) | 774, 1156, 1230 |
| EcoT22I | ATGCAT | 1 cut(s) | 435 |
| EcoT38I | GRGCYC | 1 cut(s) | 114 |
| ErhI | CCWWGG | 3 cut(s) | 774, 1156, 1230 |
| Esp3I | CGTCTC | 3 cut(s) | 1556, 1676, 1697 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1236, 1268 |
| FaqI | GGGAC | 1 cut(s) | 177 |
| FauI | CCCGC | 1 cut(s) | 220 |
| FbaI | TGATCA | 1 cut(s) | 1384 |
| Fnu4HI | GCNGC | 7 cut(s) | 865, 1052, 1055, 1080, 1191, 1248, 1521 |
| FokI | GGATG | 3 cut(s) | 331, 562, 1107 |
| FriOI | GRGCYC | 1 cut(s) | 114 |
| Fsp4HI | GCNGC | 7 cut(s) | 865, 1052, 1055, 1080, 1191, 1248, 1521 |
| FspBI | CTAG | 6 cut(s) | 18, 473, 1125, 1208, 1220, 1535 |
| GlaI | GCGC | 1 cut(s) | 1262 |
| GluI | GCNGC | 7 cut(s) | 865, 1052, 1055, 1080, 1191, 1248, 1521 |
| HaeIII | GGCC | 3 cut(s) | 1047, 1229, 1676 |
| HapII | CCGG | 1 cut(s) | 33 |
| HgaI | GACGC | 1 cut(s) | 54 |
| HhaI | GCGC | 1 cut(s) | 1263 |
| Hin1I | GRCGYC | 1 cut(s) | 46 |
| Hin6I | GCGC | 1 cut(s) | 1261 |
| HinP1I | GCGC | 1 cut(s) | 1261 |
| HincII | GTYRAC | 1 cut(s) | 84 |
| HindII | GTYRAC | 1 cut(s) | 84 |
| HindIII | AAGCTT | 2 cut(s) | 820, 1766 |
| HinfI | GANTC | 6 cut(s) | 238, 263, 638, 661, 795, 1749 |
| HpaI | GTTAAC | 1 cut(s) | 84 |
| HpaII | CCGG | 1 cut(s) | 33 |
| HphI | GGTGA | 8 cut(s) | 22, 476, 539, 1275, 1535, 1630, 1675, 1744 |
| Hpy166II | GTNNAC | 7 cut(s) | 84, 724, 781, 1420, 1581, 1638, 1762 |
| Hpy188I | TCNGA | 5 cut(s) | 237, 253, 1443, 1460, 1630 |
| Hpy188III | TCNNGA | 5 cut(s) | 92, 581, 665, 1103, 1467 |
| Hpy8I | GTNNAC | 7 cut(s) | 84, 724, 781, 1420, 1581, 1638, 1762 |
| Hpy99I | CGWCG | 1 cut(s) | 1043 |
| HpyAV | CCTTC | 4 cut(s) | 800, 908, 1433, 1751 |
| HpyCH4III | ACNGT | 3 cut(s) | 88, 543, 1567 |
| HpyCH4IV | ACGT | 2 cut(s) | 846, 1041 |
| HpyCH4V | TGCA | 5 cut(s) | 158, 433, 1520, 1581, 1608 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 689, 756, 1088, 1529, 1546 |
| HpySE526I | ACGT | 2 cut(s) | 846, 1041 |
| Hsp92I | GRCGYC | 1 cut(s) | 46 |
| HspAI | GCGC | 1 cut(s) | 1261 |
| Ksp22I | TGATCA | 1 cut(s) | 1384 |
| KspAI | GTTAAC | 1 cut(s) | 84 |
| Kzo9I | GATC | 9 cut(s) | 117, 449, 616, 715, 976, 1234, 1384, 1443, 1513 |
| LguI | GCTCTTC | 1 cut(s) | 68 |
| LmnI | GCTCC | 1 cut(s) | 1291 |
| Lsp1109I | GCAGC | 7 cut(s) | 876, 1063, 1066, 1066, 1202, 1234, 1532 |
| LweI | GCATC | 1 cut(s) | 540 |
| MaeI | CTAG | 6 cut(s) | 18, 473, 1125, 1208, 1220, 1535 |
| MaeII | ACGT | 2 cut(s) | 846, 1041 |
| MaeIII | GTNAC | 7 cut(s) | 149, 464, 527, 595, 1033, 1281, 1590 |
| MalI | GATC | 9 cut(s) | 119, 451, 618, 717, 978, 1236, 1386, 1445, 1515 |
| MboI | GATC | 9 cut(s) | 117, 449, 616, 715, 976, 1234, 1384, 1443, 1513 |
| MfeI | CAATTG | 1 cut(s) | 1654 |
| MflI | RGATCY | 3 cut(s) | 616, 715, 1443 |
| MhlI | GDGCHC | 6 cut(s) | 114, 370, 898, 1330, 1534, 1583 |
| MlsI | TGGCCA | 1 cut(s) | 1047 |
| MluNI | TGGCCA | 1 cut(s) | 1047 |
| MlyI | GAGTC | 1 cut(s) | 247 |
| MmeI | TCCRAC | 3 cut(s) | 199, 1297, 1491 |
| Mox20I | TGGCCA | 1 cut(s) | 1047 |
| Mph1103I | ATGCAT | 1 cut(s) | 435 |
| MroXI | GAANNNNTTC | 2 cut(s) | 507, 1733 |
| MscI | TGGCCA | 1 cut(s) | 1047 |
| MslI | CAYNNNNRTG | 2 cut(s) | 313, 525 |
| Msp20I | TGGCCA | 1 cut(s) | 1047 |
| MspA1I | CMGCKG | 1 cut(s) | 215 |
| MspCI | CTTAAG | 1 cut(s) | 77 |
| MspI | CCGG | 1 cut(s) | 33 |
| MspR9I | CCNGG | 3 cut(s) | 126, 285, 731 |
| MunI | CAATTG | 1 cut(s) | 1654 |
| MvaI | CCWGG | 3 cut(s) | 126, 285, 731 |
| MvnI | CGCG | 1 cut(s) | 1263 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 689, 756, 1088, 1529, 1546 |
| NdeII | GATC | 9 cut(s) | 117, 449, 616, 715, 976, 1234, 1384, 1443, 1513 |
| NlaIV | GGNNCC | 3 cut(s) | 90, 369, 1327 |
| NmeAIII | GCCGAG | 1 cut(s) | 1702 |
| NmuCI | GTSAC | 5 cut(s) | 464, 527, 595, 1033, 1281 |
| NsiI | ATGCAT | 1 cut(s) | 435 |
| NspI | RCATGY | 2 cut(s) | 437, 484 |
| NspV | TTCGAA | 1 cut(s) | 793 |
| PaeI | GCATGC | 1 cut(s) | 484 |
| PaeR7I | CTCGAG | 1 cut(s) | 485 |
| PaqCI | CACCTGC | 1 cut(s) | 1554 |
| PciSI | GCTCTTC | 1 cut(s) | 68 |
| PdmI | GAANNNNTTC | 2 cut(s) | 507, 1733 |
| PfeI | GAWTC | 5 cut(s) | 263, 638, 661, 795, 1749 |
| PfoI | TCCNGGA | 1 cut(s) | 283 |
| PkrI | GCNGC | 7 cut(s) | 866, 1053, 1056, 1081, 1192, 1249, 1522 |
| PleI | GAGTC | 1 cut(s) | 246 |
| PpsI | GAGTC | 1 cut(s) | 246 |
| PshAI | GACNNNNGTC | 1 cut(s) | 169 |
| Psp6I | CCWGG | 3 cut(s) | 124, 283, 729 |
| PspEI | GGTNACC | 1 cut(s) | 1281 |
| PspGI | CCWGG | 3 cut(s) | 124, 283, 729 |
| PspN4I | GGNNCC | 3 cut(s) | 90, 369, 1327 |
| PspPI | GGNCC | 4 cut(s) | 206, 281, 697, 1297 |
| PspXI | VCTCGAGB | 1 cut(s) | 485 |
| PsuI | RGATCY | 3 cut(s) | 616, 715, 1443 |
| RsaI | GTAC | 4 cut(s) | 193, 303, 491, 1613 |
| RsaNI | GTAC | 4 cut(s) | 192, 302, 490, 1612 |
| RseI | CAYNNNNRTG | 2 cut(s) | 313, 525 |
| SapI | GCTCTTC | 1 cut(s) | 68 |
| SatI | GCNGC | 7 cut(s) | 865, 1052, 1055, 1080, 1191, 1248, 1521 |
| Sau3AI | GATC | 9 cut(s) | 117, 449, 616, 715, 976, 1234, 1384, 1443, 1513 |
| Sau96I | GGNCC | 4 cut(s) | 206, 281, 697, 1297 |
| ScaI | AGTACT | 1 cut(s) | 491 |
| SchI | GAGTC | 1 cut(s) | 247 |
| ScrFI | CCNGG | 3 cut(s) | 126, 285, 731 |
| SduI | GDGCHC | 6 cut(s) | 114, 370, 898, 1330, 1534, 1583 |
| SfaNI | GCATC | 1 cut(s) | 540 |
| SfcI | CTRYAG | 3 cut(s) | 273, 393, 1716 |
| Sfr274I | CTCGAG | 1 cut(s) | 485 |
| SfuI | TTCGAA | 1 cut(s) | 793 |
| SinI | GGWCC | 4 cut(s) | 206, 281, 697, 1297 |
| SlaI | CTCGAG | 1 cut(s) | 485 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 313, 525 |
| SmlI | CTYRAG | 2 cut(s) | 77, 485 |
| SmoI | CTYRAG | 2 cut(s) | 77, 485 |
| SphI | GCATGC | 1 cut(s) | 484 |
| SsiI | CCGC | 5 cut(s) | 213, 727, 1204, 1263, 1726 |
| SspMI | CTAG | 6 cut(s) | 18, 473, 1125, 1208, 1220, 1535 |
| StyD4I | CCNGG | 3 cut(s) | 124, 283, 729 |
| StyI | CCWWGG | 3 cut(s) | 774, 1156, 1230 |
| TaaI | ACNGT | 3 cut(s) | 88, 543, 1567 |
| TaiI | ACGT | 2 cut(s) | 849, 1044 |
| TaqI | TCGA | 5 cut(s) | 66, 120, 486, 793, 798 |
| TaqII | GACCGA | 2 cut(s) | 614, 1052 |
| TatI | WGTACW | 2 cut(s) | 301, 489 |
| TfiI | GAWTC | 5 cut(s) | 263, 638, 661, 795, 1749 |
| TseFI | GTSAC | 5 cut(s) | 464, 527, 595, 1033, 1281 |
| TseI | GCWGC | 7 cut(s) | 864, 1051, 1054, 1079, 1190, 1247, 1520 |
| Tsp45I | GTSAC | 5 cut(s) | 464, 527, 595, 1033, 1281 |
| TspDTI | ATGAA | 3 cut(s) | 111, 898, 1020 |
| TspGWI | ACGGA | 2 cut(s) | 191, 689 |
| Vha464I | CTTAAG | 1 cut(s) | 77 |
| VneI | GTGCAC | 1 cut(s) | 1579 |
| VpaK11BI | GGWCC | 4 cut(s) | 206, 281, 697, 1297 |
| XapI | RAATTY | 5 cut(s) | 26, 131, 575, 650, 1643 |
| XceI | RCATGY | 2 cut(s) | 437, 484 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1142 |
| XhoI | CTCGAG | 1 cut(s) | 485 |
| XmnI | GAANNNNTTC | 2 cut(s) | 507, 1733 |
| XspI | CTAG | 6 cut(s) | 18, 473, 1125, 1208, 1220, 1535 |
| ZrmI | AGTACT | 1 cut(s) | 491 |
| Zsp2I | ATGCAT | 1 cut(s) | 435 |