Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2D

Orthogroup ID

OG0001528

Physical Location & Seq

Genomic Coordinates Forward (+)

5102268 .. 5109272

Gene Structure 7005 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2DG067400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1932 bp

ATGTTAGGGTCCTCCATAAACCTCATACTCCCGCCGCCGATTTCACCTTCGCCGTTTCTCTCTCTCACTCGTCTCTCTCTCCCCTACCTCCCCGCCGCCGAATTGTTGAAGGCGTCATCCAAGAAGCTTATCACTGCAACTAAGGCGCGCTCGGTGTCAACCATGGCAGCTGGAGGAGAGAATGTGGCGAGTTTGAAGCGGCAGTTAGGGCAGCTGTTTGAAGTTTCGATTAGGGCAGTGGTGCCTGATGAGCTGAATGTGGAGCCGGTGGTTGTTCCTTCGGCTGGGAAATTCGGAGACTATCAATGTAACAATGCCATGGACATATGGTCTAAGATTAAGGGAAAGGGAACTCAATTCAAGAATCCTAATTCTGTTGGACATGCCATCATGGGAAATCTTCCTAAATCAGAAATAATAGAGTCATGCACGGTAGCAGGGCCTGGGTTTGTTAATATTGTGTTGTCAAAGAATTGGATGGCTAAGAGCTTACAAAAGATGCTTATTGATGGGATCGAAACATGGGCACCACATCTCCAGGTCAGACGGGCTGTTGTAGACTTTTCCTCACCTAATATTGCCAAGGAGATGCATGTTGGCCATTTGCGATCTACAATTATCGGGGACACCCTAGCCCGGATGCTTGAGTTTTCCAAAGTTGAAGTTCTTCGGCGTAATCATGTTGGTGACTGGGGAACACAGTTTGGCATGTTGATTCAATACCTTTTCGAAAAGTTTCCAAACGTGGATGATGTAAATGAAATGGAAATCGGTGATCTTCAGGAATTCTATAAGCAATCAAAAAAGAGATTTGATGAGGATCCGGCCTTCAAGGAGAGAGCACAAGCTGCAGTGGTATCCCTTCAGGGTGGGACTCCCAAGTACCGTGAGGCATGGCTTCAAATCTGTGAAGTCAGCCGTAGAGAGTTTCAAATGGTCTATGAACGGCTTGGAACTCATTTAGAGGAAAAGGGAGAAAGCTTTTATAACCCATATATTCCAGCGGTTTTAGAGAAGTTGACTGACCAAGGACTGATTGTGGAAAGCGAGGGTGCTCGTGTAATCCATCTTGAGGGTTTTAACATCCCCCTTATTGTTGTGAAGAGCAATGGCAGTTATAATTATGCTTCCACTGATTTGGCAGCTCTTTGGTATCGACTTAATGAGGAGAAAGCTGAATGGATCATATATGTTACTGATGTTGGCCAGCAACAACACTTTAATATGTTTTTCAAGGCAGCTACACTCGCAGGTTGGCTTCCAGTTGATGGCTCGTACCCTAAAGTTAGCCATGTTGGTTTTGGTCTTGTTCTTGGAGAAGATGGAAAACGATTCCGGACTCGCTCTTCTGATGTGGTTCGATTGGTTGATTTACTTGATGAAGCCAAGAGCCGAAGCAAAACAAGTCTTGTCGAGCGTGGTAAGAGTGAAGACTGGACTGAAGAGGAGCTTGAGCAAACTGCAGTAGCTGTTGGTTATGGCGCTGTGAAGTATGCTGATCTGAAGAACAACAGATTGACCAATTACACTTTTGATTTCAATCAAATGCTAAATGATAAGGGAAACACTGCTGTTTACTTGCTATACATGCATGCTCGGATCTGTTCAATCATAAGAAAATCTGGCAAAGACATTGACGAATTGAAAAAAACGGGGGAAATTGTGTTGGGTCATGCGGGTGAGCGTGAATTGGCGCTTCACTTATTAAAGTTTTCTGAGACTGTGGAGGAGGCTTGCACGAATCTGTTGCCAAGTGTTTTATGTGAATACCTCTATAATCTATCAGAAATCTACAGCGGAAAGTTCTATTCCAATTGCCAGGTTATTGGGTCGGCAGAGGAAACGAGTAGACTCTTGCTCTGTGAAGCAACAGGCGTAGTCATGAGGAAATGCTTTCATCTTCTCGGAATTGAAGCCGTGTACAAGATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004812 GO:0004814 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006420 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0010467 GO:0016070 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0022414 GO:0032501 GO:0032502 GO:0034641 GO:0034645 GO:0034660 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0071704 GO:0090304 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

Arg_tRNA_synt_N DALR_1 tRNA-synt_1d

Protein Analysis

643

Amino Acids

71.96

Weight (kDa)

6.29

Isoelectric Point (pI)

42.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Arg_tRNA_synt_N	PF03485	71 - 155	4.2e-15	Arginyl tRNA synthetase N terminal domain
tRNA-synt_1d	PF00750	180 - 513	2.3e-117	tRNA synthetases class I (R), catalytic domain
DALR_1	PF05746	527 - 643	2.1e-29	DALR anticodon binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0001528)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G66530 AT4G26300 AT4G26300 AT4G26300 AT4G26300 AT4G26300
fragaria_vesca	FvH4_1g06400 FvH4_1g06400 FvH4_1g06400 FvH4_1g06400 FvH4_1g06400 FvH4_1g06400
malus_domestica	MD02G1067300.v1.1 MD15G1198200.v1.1 MD15G1199100.v1.1
prunus_persica	Prupe.7G218100_v2.0.a1 Prupe.7G218100_v2.0.a1 Prupe.7G218100_v2.0.a1 Prupe.7G218100_v2.0.a1
pyrus_communis	pycom02g05330 pycom15g17660
rosa_chinensis	RchiOBHm_Chr2g0092141 RchiOBHm_Chr3g0454631 RchiOBHm_Chr3g0454641 RchiOBHm_Chr7g0215671
rosa_laevigata	RLG00000016278 RLG00000025428
rosa_multiflora	Rmu_co8501171.1_g000001 Rmu_sc0000898.1_g000026 Rmu_sc0001792.1_g000032 Rmu_sc0001792.1_g000033 Rmu_sc0013552.1_g000004
rosa_roxburghii	Rroxscaffold_2G00149440 Rroxscaffold_3G00243930
rosa_rugosa	Rorug02G0023000 Rorug02G0023100 Rorug02G0023200 Rorug03G0000700 Rorug03G0000800 Rorug07G0156100
rosa_samantha	Rh2AG068800 Rh2BG068100 Rh2CG069300 Rh2DG067400 Rh3DG062300 Rh7AG292900 Rh7BG285300 Rh7CG313000 Rh7DG296400
rosa_wichuraiana	Rw2G006350 Rw7G024930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	987, 1119
Acc36I	ACCTGC	1 cut(s)	1241
AccB1I	GGYRCC	2 cut(s)	241, 526
AccB7I	CCANNNNNTGG	1 cut(s)	1268
AccI	GTMKAC	2 cut(s)	558, 1849
AccII	CGCG	1 cut(s)	148
AccIII	TCCGGA	1 cut(s)	1335
AciI	CCGC	8 cut(s)	32, 35, 93, 96, 199, 1004, 1676, 1797
AclWI	GGATC	5 cut(s)	521, 815, 828, 1190, 1607
AcoI	YGGCCR	2 cut(s)	598, 1204
AcsI	RAATTY	2 cut(s)	290, 785
AcuI	CTGAAG	4 cut(s)	764, 848, 1461, 1523
AcyI	GRCGYC	1 cut(s)	113
AfaI	GTAC	3 cut(s)	884, 1277, 1922
AfiI	CCNNNNNNNGG	4 cut(s)	284, 636, 1072, 1268
AhdI	GACNNNNNGTC	1 cut(s)	328
AjnI	CCWGG	3 cut(s)	442, 537, 1818
Alw21I	GWGCWC	2 cut(s)	844, 1057
Alw26I	GTCTC	3 cut(s)	77, 291, 1712
AlwI	GGATC	5 cut(s)	521, 815, 828, 1190, 1607
AlwNI	CAGNNNCTG	2 cut(s)	443, 1469
Aor13HI	TCCGGA	1 cut(s)	1335
AoxI	GGCC	4 cut(s)	440, 598, 825, 1204
ApeKI	GCWGC	5 cut(s)	167, 211, 848, 1142, 1238
ApoI	RAATTY	2 cut(s)	290, 785
Asp700I	GAANNNNTTC	2 cut(s)	666, 735
AspLEI	GCGC	4 cut(s)	148, 150, 1484, 1696
AspS9I	GGNCC	2 cut(s)	9, 440
AsuC2I	CCSGG	1 cut(s)	637
AsuHPI	GGTGA	5 cut(s)	36, 561, 698, 785, 1691
AsuII	TTCGAA	1 cut(s)	729
AvaII	GGWCC	1 cut(s)	9
BaeGI	GKGCMC	1 cut(s)	529
BalI	TGGCCA	2 cut(s)	600, 1206
BamHI	GGATCC	1 cut(s)	820
BanI	GGYRCC	2 cut(s)	241, 526
BauI	CACGAG	1 cut(s)	1056
BbsI	GAAGAC	1 cut(s)	1437
Bbv12I	GWGCWC	2 cut(s)	844, 1057
BbvI	GCAGC	5 cut(s)	179, 223, 835, 1154, 1250
BccI	CCATC	6 cut(s)	395, 472, 503, 1074, 1262, 1316
BceAI	ACGGC	4 cut(s)	37, 903, 962, 1901
BcgI	CGANNNNNNTGC	2 cut(s)	1729, 1763
BciT130I	CCWGG	3 cut(s)	444, 539, 1820
BciVI	GTATCC	1 cut(s)	868
BcnI	CCSGG	1 cut(s)	637
BcoDI	GTCTC	3 cut(s)	77, 291, 1712
BfaI	CTAG	1 cut(s)	632
BfmI	CTRYAG	3 cut(s)	849, 1461, 1792
BfoI	RGCGCY	2 cut(s)	1485, 1697
BfuAI	ACCTGC	1 cut(s)	1241
BfuI	GTATCC	1 cut(s)	868
BisI	GCNGC	8 cut(s)	35, 96, 168, 200, 212, 849, 1143, 1239
BlsI	GCNGC	8 cut(s)	36, 97, 169, 201, 213, 850, 1144, 1240
Bme1390I	CCNGG	4 cut(s)	444, 539, 637, 1820
Bme18I	GGWCC	1 cut(s)	9
BmeRI	GACNNNNNGTC	1 cut(s)	328
BmgT120I	GGNCC	2 cut(s)	9, 440
BmiI	GGNNCC	5 cut(s)	10, 243, 264, 528, 822
BmrFI	CCNGG	4 cut(s)	444, 539, 637, 1820
BmrI	ACTGGG	1 cut(s)	700
BmsI	GCATC	3 cut(s)	489, 579, 630
BmuI	ACTGGG	1 cut(s)	700
BpiI	GAAGAC	1 cut(s)	1437
BpmI	CTGGAG	2 cut(s)	192, 521
Bpu14I	TTCGAA	1 cut(s)	729
BpuEI	CTTGAG	3 cut(s)	665, 1091, 1472
BpuMI	CCSGG	1 cut(s)	637
BsaBI	GATNNNNATC	2 cut(s)	819, 1539
BsaHI	GRCGYC	1 cut(s)	113
BsaJI	CCNNGG	5 cut(s)	162, 318, 443, 582, 1027
BsaWI	WCCGGW	1 cut(s)	1335
BsaXI	ACNNNNNCTCC	4 cut(s)	168, 198, 519, 549
Bsc4I	CCNNNNNNNGG	4 cut(s)	284, 636, 1072, 1268
Bse118I	RCCGGY	1 cut(s)	265
Bse1I	ACTGG	3 cut(s)	695, 1262, 1439
Bse3DI	GCAATG	1 cut(s)	1114
Bse8I	GATNNNNATC	2 cut(s)	819, 1539
BseAI	TCCGGA	1 cut(s)	1335
BseBI	CCWGG	3 cut(s)	444, 539, 1820
BseDI	CCNNGG	5 cut(s)	162, 318, 443, 582, 1027
BseGI	GGATG	5 cut(s)	116, 483, 645, 754, 1083
BseJI	GATNNNNATC	2 cut(s)	819, 1539
BseLI	CCNNNNNNNGG	4 cut(s)	284, 636, 1072, 1268
BseMI	GCAATG	1 cut(s)	1114
BseMII	CTCAG	1 cut(s)	1707
BseNI	ACTGG	3 cut(s)	695, 1262, 1439
BsePI	GCGCGC	1 cut(s)	146
BseRI	GAGGAG	4 cut(s)	189, 1181, 1460, 1742
BseSI	GKGCMC	1 cut(s)	529
BseXI	GCAGC	5 cut(s)	179, 223, 835, 1154, 1250
BseYI	CCCAGC	1 cut(s)	284
Bsh1236I	CGCG	1 cut(s)	148
BshFI	GGCC	4 cut(s)	442, 600, 827, 1206
BshNI	GGYRCC	2 cut(s)	241, 526
BsiHKAI	GWGCWC	2 cut(s)	844, 1057
BsiSI	CCGG	4 cut(s)	266, 637, 824, 1336
BslFI	GGGAC	2 cut(s)	638, 886
BslI	CCNNNNNNNGG	4 cut(s)	284, 636, 1072, 1268
BsmAI	GTCTC	3 cut(s)	77, 291, 1712
BsmBI	CGTCTC	1 cut(s)	77
BsmFI	GGGAC	2 cut(s)	638, 886
BsnI	GGCC	4 cut(s)	442, 600, 827, 1206
Bsp119I	TTCGAA	1 cut(s)	729
Bsp1286I	GDGCHC	3 cut(s)	529, 844, 1057
Bsp13I	TCCGGA	1 cut(s)	1335
Bsp1407I	TGTACA	1 cut(s)	1920
Bsp143I	GATC	7 cut(s)	513, 608, 775, 820, 1182, 1498, 1599
Bsp19I	CCATGG	2 cut(s)	162, 318
BspACI	CCGC	8 cut(s)	32, 35, 93, 96, 199, 1004, 1676, 1797
BspANI	GGCC	4 cut(s)	442, 600, 827, 1206
BspCNI	CTCAG	1 cut(s)	1708
BspEI	TCCGGA	1 cut(s)	1335
BspFNI	CGCG	1 cut(s)	148
BspHI	TCATGA	1 cut(s)	1881
BspLI	GGNNCC	5 cut(s)	10, 243, 264, 528, 822
BspMAI	CTGCAG	2 cut(s)	853, 1465
BspMI	ACCTGC	1 cut(s)	1241
BspPI	GGATC	5 cut(s)	521, 815, 828, 1190, 1607
BspQI	GCTCTTC	2 cut(s)	1097, 1351
BspT104I	TTCGAA	1 cut(s)	729
BspT107I	GGYRCC	2 cut(s)	241, 526
BsrDI	GCAATG	1 cut(s)	1114
BsrFI	RCCGGY	1 cut(s)	265
BsrGI	TGTACA	1 cut(s)	1920
BsrI	ACTGG	3 cut(s)	695, 1262, 1439
BssAI	RCCGGY	1 cut(s)	265
BssECI	CCNNGG	5 cut(s)	162, 318, 443, 582, 1027
BssHII	GCGCGC	1 cut(s)	146
BssMI	GATC	7 cut(s)	513, 608, 775, 820, 1182, 1498, 1599
BssNI	GRCGYC	1 cut(s)	113
BssSI	CACGAG	1 cut(s)	1056
BssT1I	CCWWGG	4 cut(s)	162, 318, 582, 1027
Bst2BI	CACGAG	1 cut(s)	1056
Bst2UI	CCWGG	3 cut(s)	444, 539, 1820
Bst4CI	ACNGT	4 cut(s)	433, 702, 887, 1723
Bst6I	CTCTTC	3 cut(s)	1097, 1351, 1437
BstACI	GRCGYC	1 cut(s)	113
BstAPI	GCANNNNNTGC	1 cut(s)	848
BstAUI	TGTACA	1 cut(s)	1920
BstBI	TTCGAA	1 cut(s)	729
BstC8I	GCNNGC	4 cut(s)	148, 1208, 1593, 1735
BstDEI	CTNAG	4 cut(s)	141, 333, 483, 1716
BstDSI	CCRYGG	2 cut(s)	162, 318
BstF5I	GGATG	5 cut(s)	116, 483, 645, 754, 1083
BstFNI	CGCG	1 cut(s)	148
BstH2I	RGCGCY	2 cut(s)	1485, 1697
BstHHI	GCGC	4 cut(s)	148, 150, 1484, 1696
BstKTI	GATC	7 cut(s)	516, 611, 778, 823, 1185, 1501, 1602
BstMAI	GTCTC	3 cut(s)	77, 291, 1712
BstMBI	GATC	7 cut(s)	513, 608, 775, 820, 1182, 1498, 1599
BstMWI	GCNNNNNNNGC	6 cut(s)	143, 208, 250, 848, 1247, 1588
BstNI	CCWGG	3 cut(s)	444, 539, 1820
BstNSI	RCATGY	5 cut(s)	386, 596, 712, 1591, 1595
BstSCI	CCNGG	4 cut(s)	442, 537, 635, 1818
BstSFI	CTRYAG	3 cut(s)	849, 1461, 1792
BstSLI	GKGCMC	1 cut(s)	529
BstUI	CGCG	1 cut(s)	148
BstV1I	GCAGC	5 cut(s)	179, 223, 835, 1154, 1250
BstV2I	GAAGAC	1 cut(s)	1437
BstX2I	RGATCY	2 cut(s)	820, 1599
BstXI	CCANNNNNNTGG	2 cut(s)	1138, 1826
BstYI	RGATCY	2 cut(s)	820, 1599
BsuI	GTATCC	1 cut(s)	868
BsuRI	GGCC	4 cut(s)	442, 600, 827, 1206
BtgI	CCRYGG	2 cut(s)	162, 318
BtsCI	GGATG	5 cut(s)	116, 483, 645, 754, 1083
BtsI	GCAGTG	4 cut(s)	132, 243, 858, 1566
BtsIMutI	CAGTG	5 cut(s)	132, 243, 858, 1131, 1566
BveI	ACCTGC	1 cut(s)	1241
Cac8I	GCNNGC	4 cut(s)	148, 1208, 1593, 1735
CaiI	CAGNNNCTG	2 cut(s)	443, 1469
CciI	TCATGA	1 cut(s)	1881
CfoI	GCGC	4 cut(s)	148, 150, 1484, 1696
Cfr10I	RCCGGY	1 cut(s)	265
Cfr13I	GGNCC	2 cut(s)	9, 440
CseI	GACGC	1 cut(s)	102
Csp6I	GTAC	3 cut(s)	883, 1276, 1921
CviQI	GTAC	3 cut(s)	883, 1276, 1921
DdeI	CTNAG	4 cut(s)	141, 333, 483, 1716
DpnI	GATC	7 cut(s)	515, 610, 777, 822, 1184, 1500, 1601
DpnII	GATC	7 cut(s)	513, 608, 775, 820, 1182, 1498, 1599
DriI	GACNNNNNGTC	1 cut(s)	328
EaeI	YGGCCR	2 cut(s)	598, 1204
Eam1104I	CTCTTC	3 cut(s)	1097, 1351, 1437
Eam1105I	GACNNNNNGTC	1 cut(s)	328
EarI	CTCTTC	3 cut(s)	1097, 1351, 1437
Eco130I	CCWWGG	4 cut(s)	162, 318, 582, 1027
Eco47I	GGWCC	1 cut(s)	9
Eco57I	CTGAAG	4 cut(s)	764, 848, 1461, 1523
EcoO109I	RGGNCCY	2 cut(s)	9, 440
EcoRI	GAATTC	1 cut(s)	785
EcoRII	CCWGG	3 cut(s)	442, 537, 1818
EcoT14I	CCWWGG	4 cut(s)	162, 318, 582, 1027
EcoT22I	ATGCAT	2 cut(s)	594, 1593
ErhI	CCWWGG	4 cut(s)	162, 318, 582, 1027
Esp3I	CGTCTC	1 cut(s)	77
FalI	AAGNNNNNCTT	2 cut(s)	1434, 1466
FaqI	GGGAC	2 cut(s)	638, 886
FauI	CCCGC	3 cut(s)	39, 100, 1669
FauNDI	CATATG	1 cut(s)	326
FblI	GTMKAC	2 cut(s)	558, 1849
Fnu4HI	GCNGC	8 cut(s)	35, 96, 168, 200, 212, 849, 1143, 1239
FokI	GGATG	5 cut(s)	103, 490, 652, 761, 1070
Fsp4HI	GCNGC	8 cut(s)	35, 96, 168, 200, 212, 849, 1143, 1239
FspBI	CTAG	1 cut(s)	632
GlaI	GCGC	4 cut(s)	147, 149, 1483, 1695
GluI	GCNGC	8 cut(s)	35, 96, 168, 200, 212, 849, 1143, 1239
GsaI	CCCAGC	1 cut(s)	288
GsuI	CTGGAG	2 cut(s)	192, 521
HaeII	RGCGCY	2 cut(s)	1485, 1697
HaeIII	GGCC	4 cut(s)	442, 600, 827, 1206
HapII	CCGG	4 cut(s)	266, 637, 824, 1336
HgaI	GACGC	1 cut(s)	102
HhaI	GCGC	4 cut(s)	148, 150, 1484, 1696
Hin1I	GRCGYC	1 cut(s)	113
Hin6I	GCGC	4 cut(s)	146, 148, 1482, 1694
HinP1I	GCGC	4 cut(s)	146, 148, 1482, 1694
HincII	GTYRAC	2 cut(s)	159, 1020
HindII	GTYRAC	2 cut(s)	159, 1020
HindIII	AAGCTT	2 cut(s)	125, 979
HinfI	GANTC	8 cut(s)	364, 422, 715, 874, 1332, 1339, 1741, 1851
HpaII	CCGG	4 cut(s)	266, 637, 824, 1336
HphI	GGTGA	5 cut(s)	36, 561, 698, 785, 1691
Hpy166II	GTNNAC	6 cut(s)	159, 559, 1020, 1576, 1850, 1921
Hpy188I	TCNGA	9 cut(s)	296, 412, 545, 1351, 1503, 1599, 1717, 1786, 1907
Hpy188III	TCNNGA	5 cut(s)	361, 782, 1070, 1336, 1882
Hpy8I	GTNNAC	6 cut(s)	159, 559, 1020, 1576, 1850, 1921
HpyAV	CCTTC	5 cut(s)	57, 103, 288, 838, 872
HpyCH4III	ACNGT	4 cut(s)	433, 702, 887, 1723
HpyCH4IV	ACGT	1 cut(s)	744
HpyCH4V	TGCA	7 cut(s)	137, 429, 592, 851, 1463, 1591, 1737
HpyF10VI	GCNNNNNNNGC	6 cut(s)	143, 208, 250, 848, 1247, 1588
HpyF3I	CTNAG	4 cut(s)	141, 333, 483, 1716
HpySE526I	ACGT	1 cut(s)	744
Hsp92I	GRCGYC	1 cut(s)	113
HspAI	GCGC	4 cut(s)	146, 148, 1482, 1694
Kpn2I	TCCGGA	1 cut(s)	1335
Kzo9I	GATC	7 cut(s)	513, 608, 775, 820, 1182, 1498, 1599
LguI	GCTCTTC	2 cut(s)	1097, 1351
LmnI	GCTCC	2 cut(s)	262, 1447
Lsp1109I	GCAGC	5 cut(s)	179, 223, 835, 1154, 1250
LweI	GCATC	3 cut(s)	489, 579, 630
MaeI	CTAG	1 cut(s)	632
MaeII	ACGT	1 cut(s)	744
MaeIII	GTNAC	3 cut(s)	308, 686, 1192
MalI	GATC	7 cut(s)	515, 610, 777, 822, 1184, 1500, 1601
MboI	GATC	7 cut(s)	513, 608, 775, 820, 1182, 1498, 1599
MfeI	CAATTG	1 cut(s)	1813
MflI	RGATCY	2 cut(s)	820, 1599
MhlI	GDGCHC	3 cut(s)	529, 844, 1057
MlsI	TGGCCA	2 cut(s)	600, 1206
MluNI	TGGCCA	2 cut(s)	600, 1206
MlyI	GAGTC	4 cut(s)	431, 868, 1333, 1845
MmeI	TCCRAC	1 cut(s)	358
Mox20I	TGGCCA	2 cut(s)	600, 1206
Mph1103I	ATGCAT	2 cut(s)	594, 1593
MroI	TCCGGA	1 cut(s)	1335
MroXI	GAANNNNTTC	2 cut(s)	666, 735
MscI	TGGCCA	2 cut(s)	600, 1206
MseI	TTAA	6 cut(s)	339, 453, 1080, 1161, 1221, 1706
MslI	CAYNNNNRTG	2 cut(s)	684, 1677
Msp20I	TGGCCA	2 cut(s)	600, 1206
MspA1I	CMGCKG	4 cut(s)	170, 214, 1004, 1797
MspI	CCGG	4 cut(s)	266, 637, 824, 1336
MspR9I	CCNGG	4 cut(s)	444, 539, 637, 1820
MunI	CAATTG	1 cut(s)	1813
MvaI	CCWGG	3 cut(s)	444, 539, 1820
MvnI	CGCG	1 cut(s)	148
MwoI	GCNNNNNNNGC	6 cut(s)	143, 208, 250, 848, 1247, 1588
NciI	CCSGG	1 cut(s)	637
NcoI	CCATGG	2 cut(s)	162, 318
NdeI	CATATG	1 cut(s)	326
NdeII	GATC	7 cut(s)	513, 608, 775, 820, 1182, 1498, 1599
NlaIV	GGNNCC	5 cut(s)	10, 243, 264, 528, 822
NmuCI	GTSAC	1 cut(s)	686
NsiI	ATGCAT	2 cut(s)	594, 1593
NspI	RCATGY	5 cut(s)	386, 596, 712, 1591, 1595
NspV	TTCGAA	1 cut(s)	729
PaeI	GCATGC	1 cut(s)	1595
PagI	TCATGA	1 cut(s)	1881
PauI	GCGCGC	1 cut(s)	146
PciSI	GCTCTTC	2 cut(s)	1097, 1351
PdmI	GAANNNNTTC	2 cut(s)	666, 735
PfeI	GAWTC	4 cut(s)	364, 715, 1332, 1741
PflMI	CCANNNNNTGG	1 cut(s)	1268
PkrI	GCNGC	8 cut(s)	36, 97, 169, 201, 213, 850, 1144, 1240
PleI	GAGTC	4 cut(s)	430, 868, 1333, 1845
PpsI	GAGTC	4 cut(s)	430, 868, 1333, 1845
PpuMI	RGGWCCY	1 cut(s)	9
PsiI	TTATAA	2 cut(s)	987, 1119
Psp5II	RGGWCCY	1 cut(s)	9
Psp6I	CCWGG	3 cut(s)	442, 537, 1818
PspFI	CCCAGC	1 cut(s)	284
PspGI	CCWGG	3 cut(s)	442, 537, 1818
PspN4I	GGNNCC	5 cut(s)	10, 243, 264, 528, 822
PspPI	GGNCC	2 cut(s)	9, 440
PspPPI	RGGWCCY	1 cut(s)	9
PstI	CTGCAG	2 cut(s)	853, 1465
PstNI	CAGNNNCTG	2 cut(s)	443, 1469
PsuI	RGATCY	2 cut(s)	820, 1599
PteI	GCGCGC	1 cut(s)	146
PvuII	CAGCTG	2 cut(s)	170, 214
RsaI	GTAC	3 cut(s)	884, 1277, 1922
RsaNI	GTAC	3 cut(s)	883, 1276, 1921
RseI	CAYNNNNRTG	2 cut(s)	684, 1677
SapI	GCTCTTC	2 cut(s)	1097, 1351
SaqAI	TTAA	6 cut(s)	339, 453, 1080, 1161, 1221, 1706
SatI	GCNGC	8 cut(s)	35, 96, 168, 200, 212, 849, 1143, 1239
Sau3AI	GATC	7 cut(s)	513, 608, 775, 820, 1182, 1498, 1599
Sau96I	GGNCC	2 cut(s)	9, 440
SchI	GAGTC	4 cut(s)	431, 868, 1333, 1845
ScrFI	CCNGG	4 cut(s)	444, 539, 637, 1820
SduI	GDGCHC	3 cut(s)	529, 844, 1057
SfaNI	GCATC	3 cut(s)	489, 579, 630
SfcI	CTRYAG	3 cut(s)	849, 1461, 1792
SfuI	TTCGAA	1 cut(s)	729
SinI	GGWCC	1 cut(s)	9
SmiMI	CAYNNNNRTG	2 cut(s)	684, 1677
SmlI	CTYRAG	3 cut(s)	644, 1070, 1451
SmoI	CTYRAG	3 cut(s)	644, 1070, 1451
SphI	GCATGC	1 cut(s)	1595
SsiI	CCGC	8 cut(s)	32, 35, 93, 96, 199, 1004, 1676, 1797
SspI	AATATT	2 cut(s)	457, 577
SspMI	CTAG	1 cut(s)	632
StyD4I	CCNGG	4 cut(s)	442, 537, 635, 1818
StyI	CCWWGG	4 cut(s)	162, 318, 582, 1027
TaaI	ACNGT	4 cut(s)	433, 702, 887, 1723
TaiI	ACGT	1 cut(s)	747
TaqI	TCGA	6 cut(s)	227, 516, 729, 1156, 1360, 1413
TatI	WGTACW	1 cut(s)	1920
TauI	GCSGC	3 cut(s)	37, 98, 202
TfiI	GAWTC	4 cut(s)	364, 715, 1332, 1741
Tru1I	TTAA	6 cut(s)	339, 453, 1080, 1161, 1221, 1706
Tru9I	TTAA	6 cut(s)	339, 453, 1080, 1161, 1221, 1706
TscAI	CASTG	5 cut(s)	139, 243, 858, 1138, 1573
TseFI	GTSAC	1 cut(s)	686
TseI	GCWGC	5 cut(s)	167, 211, 848, 1142, 1238
Tsp45I	GTSAC	1 cut(s)	686
TspDTI	ATGAA	4 cut(s)	774, 957, 1395, 1886
TspRI	CASTG	5 cut(s)	139, 243, 858, 1138, 1573
Van91I	CCANNNNNTGG	1 cut(s)	1268
VpaK11BI	GGWCC	1 cut(s)	9
XapI	RAATTY	2 cut(s)	290, 785
XceI	RCATGY	5 cut(s)	386, 596, 712, 1591, 1595
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1298
XmiI	GTMKAC	2 cut(s)	558, 1849
XmnI	GAANNNNTTC	2 cut(s)	666, 735
XspI	CTAG	1 cut(s)	632
Zsp2I	ATGCAT	2 cut(s)	594, 1593

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.