Belongs to the class-I aminoacyl-tRNA synthetase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 1002, 1134 |
| Acc36I | ACCTGC | 2 cut(s) | 1256, 1760 |
| AccB1I | GGYRCC | 2 cut(s) | 541, 1067 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 392 |
| AccBSI | CCGCTC | 1 cut(s) | 1702 |
| AccI | GTMKAC | 1 cut(s) | 52 |
| AccII | CGCG | 1 cut(s) | 1437 |
| AciI | CCGC | 6 cut(s) | 87, 90, 901, 1128, 1437, 1702 |
| AclWI | GGATC | 8 cut(s) | 3, 16, 500, 830, 1205, 1416, 1625, 1695 |
| AcoI | YGGCCR | 3 cut(s) | 613, 780, 1219 |
| AcsI | RAATTY | 2 cut(s) | 305, 941 |
| AcuI | CTGAAG | 4 cut(s) | 207, 536, 1467, 1541 |
| AcyI | GRCGYC | 1 cut(s) | 542 |
| AfaI | GTAC | 3 cut(s) | 367, 655, 899 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 392, 424, 1180 |
| AflII | CTTAAG | 1 cut(s) | 619 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 580 |
| AjnI | CCWGG | 3 cut(s) | 457, 1015, 1836 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 594, 626, 768, 800 |
| AluBI | AGCT | 9 cut(s) | 121, 229, 550, 596, 996, 1160, 1190, 1256, 1468 |
| AluI | AGCT | 9 cut(s) | 121, 229, 550, 596, 996, 1160, 1190, 1256, 1468 |
| Alw26I | GTCTC | 2 cut(s) | 1742, 1854 |
| AlwI | GGATC | 8 cut(s) | 3, 16, 500, 830, 1205, 1416, 1625, 1695 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 458, 1487 |
| Ama87I | CYCGRG | 1 cut(s) | 1071 |
| AoxI | GGCC | 5 cut(s) | 379, 399, 613, 780, 1219 |
| ApaI | GGGCCC | 1 cut(s) | 383 |
| ApeKI | GCWGC | 4 cut(s) | 107, 1225, 1228, 1253 |
| ApoI | RAATTY | 2 cut(s) | 305, 941 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1860, 1892 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 681, 1910 |
| AspLEI | GCGC | 5 cut(s) | 31, 299, 544, 859, 1437 |
| AspS9I | GGNCC | 4 cut(s) | 379, 380, 455, 1375 |
| AsuC2I | CCSGG | 3 cut(s) | 301, 1072, 1073 |
| AsuHPI | GGTGA | 5 cut(s) | 576, 713, 800, 1346, 1709 |
| AsuNHI | GCTAGC | 1 cut(s) | 646 |
| AvaI | CYCGRG | 1 cut(s) | 1071 |
| AvaII | GGWCC | 2 cut(s) | 455, 1375 |
| BaeGI | GKGCMC | 2 cut(s) | 383, 1072 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 881, 914 |
| BalI | TGGCCA | 2 cut(s) | 615, 1221 |
| BamHI | GGATCC | 1 cut(s) | 8 |
| BanI | GGYRCC | 2 cut(s) | 541, 1067 |
| BanII | GRGCYC | 1 cut(s) | 383 |
| BbvI | GCAGC | 4 cut(s) | 119, 1237, 1240, 1265 |
| BccI | CCATC | 6 cut(s) | 172, 394, 410, 518, 1118, 1334 |
| BceAI | ACGGC | 2 cut(s) | 795, 918 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 42, 76 |
| BciT130I | CCWGG | 3 cut(s) | 459, 1017, 1838 |
| BciVI | GTATCC | 1 cut(s) | 181 |
| BclI | TGATCA | 2 cut(s) | 1471, 1558 |
| BcnI | CCSGG | 3 cut(s) | 301, 1072, 1073 |
| BcoDI | GTCTC | 2 cut(s) | 1742, 1854 |
| BfaI | CTAG | 4 cut(s) | 114, 647, 1325, 1394 |
| BfmI | CTRYAG | 5 cut(s) | 447, 794, 878, 1479, 1724 |
| BfoI | RGCGCY | 2 cut(s) | 32, 545 |
| BfrI | CTTAAG | 1 cut(s) | 619 |
| BfuAI | ACCTGC | 2 cut(s) | 1256, 1760 |
| BfuI | GTATCC | 1 cut(s) | 181 |
| BisI | GCNGC | 5 cut(s) | 90, 108, 1226, 1229, 1254 |
| BlsI | GCNGC | 5 cut(s) | 91, 109, 1227, 1230, 1255 |
| Bme1390I | CCNGG | 6 cut(s) | 301, 459, 1017, 1072, 1073, 1838 |
| Bme18I | GGWCC | 2 cut(s) | 455, 1375 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 580 |
| BmeT110I | CYCGRG | 1 cut(s) | 1071 |
| BmgT120I | GGNCC | 4 cut(s) | 379, 380, 455, 1375 |
| BmiI | GGNNCC | 6 cut(s) | 10, 381, 543, 891, 1069, 1274 |
| BmrFI | CCNGG | 6 cut(s) | 301, 459, 1017, 1072, 1073, 1838 |
| BmrI | ACTGGG | 1 cut(s) | 715 |
| BmsI | GCATC | 4 cut(s) | 119, 504, 821, 1554 |
| BmtI | GCTAGC | 1 cut(s) | 650 |
| BmuI | ACTGGG | 1 cut(s) | 715 |
| BpmI | CTGGAG | 1 cut(s) | 1836 |
| BpuEI | CTTGAG | 3 cut(s) | 680, 1348, 1451 |
| BpuMI | CCSGG | 3 cut(s) | 301, 1072, 1073 |
| BsaBI | GATNNNNATC | 2 cut(s) | 834, 1557 |
| BsaHI | GRCGYC | 1 cut(s) | 542 |
| BsaI | GGTCTC | 2 cut(s) | 1742, 1854 |
| BsaJI | CCNNGG | 5 cut(s) | 333, 964, 1016, 1071, 1404 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 392, 424, 1180 |
| Bse118I | RCCGGY | 1 cut(s) | 280 |
| Bse1I | ACTGG | 1 cut(s) | 710 |
| Bse3DI | GCAATG | 1 cut(s) | 910 |
| Bse8I | GATNNNNATC | 2 cut(s) | 834, 1557 |
| BseBI | CCWGG | 3 cut(s) | 459, 1017, 1838 |
| BseDI | CCNNGG | 5 cut(s) | 333, 964, 1016, 1071, 1404 |
| BseGI | GGATG | 1 cut(s) | 1098 |
| BseJI | GATNNNNATC | 2 cut(s) | 834, 1557 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 392, 424, 1180 |
| BseMI | GCAATG | 1 cut(s) | 910 |
| BseMII | CTCAG | 4 cut(s) | 61, 159, 180, 259 |
| BseNI | ACTGG | 1 cut(s) | 710 |
| BseRI | GAGGAG | 3 cut(s) | 995, 1196, 1760 |
| BseSI | GKGCMC | 2 cut(s) | 383, 1072 |
| BseXI | GCAGC | 4 cut(s) | 119, 1237, 1240, 1265 |
| BsgI | GTGCAG | 2 cut(s) | 1284, 1738 |
| Bsh1236I | CGCG | 1 cut(s) | 1437 |
| BshFI | GGCC | 5 cut(s) | 381, 401, 615, 782, 1221 |
| BshNI | GGYRCC | 2 cut(s) | 541, 1067 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1071 |
| BsiSI | CCGG | 3 cut(s) | 281, 300, 1072 |
| BslFI | GGGAC | 4 cut(s) | 68, 242, 653, 724 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 392, 424, 1180 |
| BsmAI | GTCTC | 2 cut(s) | 1742, 1854 |
| BsmFI | GGGAC | 4 cut(s) | 68, 242, 653, 724 |
| BsmI | GAATGC | 1 cut(s) | 800 |
| BsnI | GGCC | 5 cut(s) | 381, 401, 615, 782, 1221 |
| Bso31I | GGTCTC | 2 cut(s) | 1742, 1854 |
| BsoBI | CYCGRG | 1 cut(s) | 1071 |
| Bsp120I | GGGCCC | 1 cut(s) | 379 |
| Bsp1286I | GDGCHC | 2 cut(s) | 383, 1072 |
| Bsp19I | CCATGG | 1 cut(s) | 333 |
| BspACI | CCGC | 6 cut(s) | 87, 90, 901, 1128, 1437, 1702 |
| BspANI | GGCC | 5 cut(s) | 381, 401, 615, 782, 1221 |
| BspCNI | CTCAG | 4 cut(s) | 60, 158, 179, 258 |
| BspFNI | CGCG | 1 cut(s) | 1437 |
| BspHI | TCATGA | 1 cut(s) | 405 |
| BspLI | GGNNCC | 6 cut(s) | 10, 381, 543, 891, 1069, 1274 |
| BspMAI | CTGCAG | 3 cut(s) | 798, 882, 1483 |
| BspMI | ACCTGC | 2 cut(s) | 1256, 1760 |
| BspOI | GCTAGC | 1 cut(s) | 650 |
| BspPI | GGATC | 8 cut(s) | 3, 16, 500, 830, 1205, 1416, 1625, 1695 |
| BspT107I | GGYRCC | 2 cut(s) | 541, 1067 |
| BspTI | CTTAAG | 1 cut(s) | 619 |
| BspTNI | GGTCTC | 2 cut(s) | 1742, 1854 |
| BsrBI | CCGCTC | 1 cut(s) | 1702 |
| BsrDI | GCAATG | 1 cut(s) | 910 |
| BsrFI | RCCGGY | 1 cut(s) | 280 |
| BsrI | ACTGG | 1 cut(s) | 710 |
| BssAI | RCCGGY | 1 cut(s) | 280 |
| BssECI | CCNNGG | 5 cut(s) | 333, 964, 1016, 1071, 1404 |
| BssNI | GRCGYC | 1 cut(s) | 542 |
| BssT1I | CCWWGG | 3 cut(s) | 333, 964, 1404 |
| Bst2UI | CCWGG | 3 cut(s) | 459, 1017, 1838 |
| Bst4CI | ACNGT | 5 cut(s) | 219, 717, 1499, 1728, 1872 |
| Bst6I | CTCTTC | 1 cut(s) | 1443 |
| BstACI | GRCGYC | 1 cut(s) | 542 |
| BstAFI | CTTAAG | 1 cut(s) | 619 |
| BstC8I | GCNNGC | 5 cut(s) | 295, 648, 798, 878, 1223 |
| BstDEI | CTNAG | 6 cut(s) | 47, 145, 166, 245, 498, 894 |
| BstDSI | CCRYGG | 1 cut(s) | 333 |
| BstEII | GGTNACC | 1 cut(s) | 1303 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1178 |
| BstF5I | GGATG | 1 cut(s) | 1098 |
| BstFNI | CGCG | 1 cut(s) | 1437 |
| BstH2I | RGCGCY | 2 cut(s) | 32, 545 |
| BstHHI | GCGC | 5 cut(s) | 31, 299, 544, 859, 1437 |
| BstMAI | GTCTC | 2 cut(s) | 1742, 1854 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 863, 1262, 1720 |
| BstNI | CCWGG | 3 cut(s) | 459, 1017, 1838 |
| BstNSI | RCATGY | 1 cut(s) | 611 |
| BstPI | GGTNACC | 1 cut(s) | 1303 |
| BstSCI | CCNGG | 6 cut(s) | 299, 457, 1015, 1070, 1071, 1836 |
| BstSFI | CTRYAG | 5 cut(s) | 447, 794, 878, 1479, 1724 |
| BstSLI | GKGCMC | 2 cut(s) | 383, 1072 |
| BstUI | CGCG | 1 cut(s) | 1437 |
| BstV1I | GCAGC | 4 cut(s) | 119, 1237, 1240, 1265 |
| BstX2I | RGATCY | 2 cut(s) | 8, 1617 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1844 |
| BstYI | RGATCY | 2 cut(s) | 8, 1617 |
| BsuI | GTATCC | 1 cut(s) | 181 |
| BsuRI | GGCC | 5 cut(s) | 381, 401, 615, 782, 1221 |
| BtgI | CCRYGG | 1 cut(s) | 333 |
| BtsCI | GGATG | 1 cut(s) | 1098 |
| BtsI | GCAGTG | 2 cut(s) | 873, 1584 |
| BtsIMutI | CAGTG | 3 cut(s) | 873, 1504, 1584 |
| BveI | ACCTGC | 2 cut(s) | 1256, 1760 |
| Cac8I | GCNNGC | 5 cut(s) | 295, 648, 798, 878, 1223 |
| CaiI | CAGNNNCTG | 2 cut(s) | 458, 1487 |
| CciI | TCATGA | 1 cut(s) | 405 |
| CfoI | GCGC | 5 cut(s) | 31, 299, 544, 859, 1437 |
| Cfr10I | RCCGGY | 1 cut(s) | 280 |
| Cfr13I | GGNCC | 4 cut(s) | 379, 380, 455, 1375 |
| Cfr9I | CCCGGG | 1 cut(s) | 1071 |
| CseI | GACGC | 2 cut(s) | 148, 169 |
| Csp6I | GTAC | 3 cut(s) | 366, 654, 898 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1353, 1388 |
| CviAII | CATG | 8 cut(s) | 334, 406, 441, 608, 695, 909, 1310, 1610 |
| CviQI | GTAC | 3 cut(s) | 366, 654, 898 |
| DdeI | CTNAG | 6 cut(s) | 47, 145, 166, 245, 498, 894 |
| DinI | GGCGCC | 1 cut(s) | 543 |
| DraI | TTTAAA | 1 cut(s) | 1026 |
| DriI | GACNNNNNGTC | 1 cut(s) | 580 |
| EaeI | YGGCCR | 3 cut(s) | 613, 780, 1219 |
| Eam1104I | CTCTTC | 1 cut(s) | 1443 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 580 |
| EarI | CTCTTC | 1 cut(s) | 1443 |
| Eco130I | CCWWGG | 3 cut(s) | 333, 964, 1404 |
| Eco24I | GRGCYC | 1 cut(s) | 383 |
| Eco31I | GGTCTC | 2 cut(s) | 1742, 1854 |
| Eco47I | GGWCC | 2 cut(s) | 455, 1375 |
| Eco57I | CTGAAG | 4 cut(s) | 207, 536, 1467, 1541 |
| Eco88I | CYCGRG | 1 cut(s) | 1071 |
| Eco91I | GGTNACC | 1 cut(s) | 1303 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1178 |
| EcoO109I | RGGNCCY | 3 cut(s) | 379, 380, 455 |
| EcoO65I | GGTNACC | 1 cut(s) | 1303 |
| EcoRII | CCWGG | 3 cut(s) | 457, 1015, 1836 |
| EcoT14I | CCWWGG | 3 cut(s) | 333, 964, 1404 |
| EcoT22I | ATGCAT | 2 cut(s) | 609, 910 |
| EcoT38I | GRGCYC | 1 cut(s) | 383 |
| EgeI | GGCGCC | 1 cut(s) | 543 |
| EheI | GGCGCC | 1 cut(s) | 543 |
| ErhI | CCWWGG | 3 cut(s) | 333, 964, 1404 |
| FaeI | CATG | 8 cut(s) | 337, 409, 444, 611, 698, 912, 1313, 1613 |
| FaqI | GGGAC | 4 cut(s) | 68, 242, 653, 724 |
| FatI | CATG | 8 cut(s) | 333, 405, 440, 607, 694, 908, 1309, 1609 |
| FauI | CCCGC | 2 cut(s) | 94, 1695 |
| FauNDI | CATATG | 1 cut(s) | 1692 |
| FbaI | TGATCA | 2 cut(s) | 1471, 1558 |
| FblI | GTMKAC | 1 cut(s) | 52 |
| Fnu4HI | GCNGC | 5 cut(s) | 90, 108, 1226, 1229, 1254 |
| FokI | GGATG | 1 cut(s) | 1085 |
| FriOI | GRGCYC | 1 cut(s) | 383 |
| Fsp4HI | GCNGC | 5 cut(s) | 90, 108, 1226, 1229, 1254 |
| FspBI | CTAG | 4 cut(s) | 114, 647, 1325, 1394 |
| GlaI | GCGC | 5 cut(s) | 30, 298, 543, 858, 1436 |
| GluI | GCNGC | 5 cut(s) | 90, 108, 1226, 1229, 1254 |
| GsuI | CTGGAG | 1 cut(s) | 1836 |
| HaeII | RGCGCY | 2 cut(s) | 32, 545 |
| HaeIII | GGCC | 5 cut(s) | 381, 401, 615, 782, 1221 |
| HapII | CCGG | 3 cut(s) | 281, 300, 1072 |
| HgaI | GACGC | 2 cut(s) | 148, 169 |
| HhaI | GCGC | 5 cut(s) | 31, 299, 544, 859, 1437 |
| Hin1I | GRCGYC | 1 cut(s) | 542 |
| Hin1II | CATG | 8 cut(s) | 337, 409, 444, 611, 698, 912, 1313, 1613 |
| Hin6I | GCGC | 5 cut(s) | 29, 297, 542, 857, 1435 |
| HinP1I | GCGC | 5 cut(s) | 29, 297, 542, 857, 1435 |
| HincII | GTYRAC | 3 cut(s) | 53, 574, 627 |
| HindII | GTYRAC | 3 cut(s) | 53, 574, 627 |
| HindIII | AAGCTT | 2 cut(s) | 119, 994 |
| HinfI | GANTC | 6 cut(s) | 54, 437, 691, 825, 1357, 1926 |
| HpaII | CCGG | 3 cut(s) | 281, 300, 1072 |
| HphI | GGTGA | 5 cut(s) | 576, 713, 800, 1346, 1709 |
| Hpy166II | GTNNAC | 6 cut(s) | 53, 574, 627, 1455, 1594, 1939 |
| Hpy188III | TCNNGA | 7 cut(s) | 114, 406, 659, 1085, 1117, 1430, 1853 |
| Hpy8I | GTNNAC | 6 cut(s) | 53, 574, 627, 1455, 1594, 1939 |
| Hpy99I | CGWCG | 1 cut(s) | 690 |
| HpyAV | CCTTC | 5 cut(s) | 51, 97, 979, 1082, 1436 |
| HpyCH4III | ACNGT | 5 cut(s) | 219, 717, 1499, 1728, 1872 |
| HpyCH4IV | ACGT | 3 cut(s) | 759, 1261, 1740 |
| HpyCH4V | TGCA | 9 cut(s) | 131, 607, 796, 880, 908, 973, 1265, 1481, 1755 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 863, 1262, 1720 |
| HpyF3I | CTNAG | 6 cut(s) | 47, 145, 166, 245, 498, 894 |
| HpySE526I | ACGT | 3 cut(s) | 759, 1261, 1740 |
| Hsp92I | GRCGYC | 1 cut(s) | 542 |
| Hsp92II | CATG | 8 cut(s) | 337, 409, 444, 611, 698, 912, 1313, 1613 |
| HspAI | GCGC | 5 cut(s) | 29, 297, 542, 857, 1435 |
| KasI | GGCGCC | 1 cut(s) | 541 |
| Ksp22I | TGATCA | 2 cut(s) | 1471, 1558 |
| LmnI | GCTCC | 2 cut(s) | 1278, 1465 |
| Lsp1109I | GCAGC | 4 cut(s) | 119, 1237, 1240, 1265 |
| LweI | GCATC | 4 cut(s) | 119, 504, 821, 1554 |
| MaeI | CTAG | 4 cut(s) | 114, 647, 1325, 1394 |
| MaeII | ACGT | 3 cut(s) | 759, 1261, 1740 |
| MaeIII | GTNAC | 6 cut(s) | 323, 582, 701, 1207, 1303, 1493 |
| MbiI | CCGCTC | 1 cut(s) | 1702 |
| MboII | GAAGA | 8 cut(s) | 69, 200, 203, 674, 776, 1349, 1460, 1534 |
| MfeI | CAATTG | 1 cut(s) | 1831 |
| MflI | RGATCY | 2 cut(s) | 8, 1617 |
| MhlI | GDGCHC | 2 cut(s) | 383, 1072 |
| MlsI | TGGCCA | 2 cut(s) | 615, 1221 |
| MluCI | AATT | 8 cut(s) | 305, 941, 1050, 1540, 1658, 1677, 1706, 1831 |
| MluNI | TGGCCA | 2 cut(s) | 615, 1221 |
| Mly113I | GGCGCC | 1 cut(s) | 542 |
| MlyI | GAGTC | 2 cut(s) | 48, 1351 |
| MmeI | TCCRAC | 2 cut(s) | 373, 1665 |
| Mox20I | TGGCCA | 2 cut(s) | 615, 1221 |
| Mph1103I | ATGCAT | 2 cut(s) | 609, 910 |
| MroXI | GAANNNNTTC | 2 cut(s) | 681, 1910 |
| MscI | TGGCCA | 2 cut(s) | 615, 1221 |
| MslI | CAYNNNNRTG | 3 cut(s) | 699, 1695, 1898 |
| Msp20I | TGGCCA | 2 cut(s) | 615, 1221 |
| MspA1I | CMGCKG | 1 cut(s) | 92 |
| MspCI | CTTAAG | 1 cut(s) | 619 |
| MspI | CCGG | 3 cut(s) | 281, 300, 1072 |
| MspR9I | CCNGG | 6 cut(s) | 301, 459, 1017, 1072, 1073, 1838 |
| MunI | CAATTG | 1 cut(s) | 1831 |
| Mva1269I | GAATGC | 1 cut(s) | 800 |
| MvaI | CCWGG | 3 cut(s) | 459, 1017, 1838 |
| MvnI | CGCG | 1 cut(s) | 1437 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 863, 1262, 1720 |
| NarI | GGCGCC | 1 cut(s) | 542 |
| NciI | CCSGG | 3 cut(s) | 301, 1072, 1073 |
| NcoI | CCATGG | 1 cut(s) | 333 |
| NdeI | CATATG | 1 cut(s) | 1692 |
| NheI | GCTAGC | 1 cut(s) | 646 |
| NlaIII | CATG | 8 cut(s) | 337, 409, 444, 611, 698, 912, 1313, 1613 |
| NlaIV | GGNNCC | 6 cut(s) | 10, 381, 543, 891, 1069, 1274 |
| NmuCI | GTSAC | 2 cut(s) | 582, 701 |
| NsiI | ATGCAT | 2 cut(s) | 609, 910 |
| NspI | RCATGY | 1 cut(s) | 611 |
| PagI | TCATGA | 1 cut(s) | 405 |
| PctI | GAATGC | 1 cut(s) | 800 |
| PdmI | GAANNNNTTC | 2 cut(s) | 681, 1910 |
| PfeI | GAWTC | 4 cut(s) | 437, 691, 825, 1926 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 392 |
| PkrI | GCNGC | 5 cut(s) | 91, 109, 1227, 1230, 1255 |
| PleI | GAGTC | 2 cut(s) | 48, 1351 |
| PluTI | GGCGCC | 1 cut(s) | 545 |
| PpsI | GAGTC | 2 cut(s) | 48, 1351 |
| PpuMI | RGGWCCY | 1 cut(s) | 455 |
| PsiI | TTATAA | 2 cut(s) | 1002, 1134 |
| Psp5II | RGGWCCY | 1 cut(s) | 455 |
| Psp6I | CCWGG | 3 cut(s) | 457, 1015, 1836 |
| PspEI | GGTNACC | 1 cut(s) | 1303 |
| PspGI | CCWGG | 3 cut(s) | 457, 1015, 1836 |
| PspN4I | GGNNCC | 6 cut(s) | 10, 381, 543, 891, 1069, 1274 |
| PspOMI | GGGCCC | 1 cut(s) | 379 |
| PspPI | GGNCC | 4 cut(s) | 379, 380, 455, 1375 |
| PspPPI | RGGWCCY | 1 cut(s) | 455 |
| PstI | CTGCAG | 3 cut(s) | 798, 882, 1483 |
| PstNI | CAGNNNCTG | 2 cut(s) | 458, 1487 |
| PsuI | RGATCY | 2 cut(s) | 8, 1617 |
| RsaI | GTAC | 3 cut(s) | 367, 655, 899 |
| RsaNI | GTAC | 3 cut(s) | 366, 654, 898 |
| RseI | CAYNNNNRTG | 3 cut(s) | 699, 1695, 1898 |
| SalI | GTCGAC | 1 cut(s) | 51 |
| SatI | GCNGC | 5 cut(s) | 90, 108, 1226, 1229, 1254 |
| Sau96I | GGNCC | 4 cut(s) | 379, 380, 455, 1375 |
| SbfI | CCTGCAGG | 1 cut(s) | 882 |
| SchI | GAGTC | 2 cut(s) | 48, 1351 |
| ScrFI | CCNGG | 6 cut(s) | 301, 459, 1017, 1072, 1073, 1838 |
| SdaI | CCTGCAGG | 1 cut(s) | 882 |
| SduI | GDGCHC | 2 cut(s) | 383, 1072 |
| SfaNI | GCATC | 4 cut(s) | 119, 504, 821, 1554 |
| SfcI | CTRYAG | 5 cut(s) | 447, 794, 878, 1479, 1724 |
| SfoI | GGCGCC | 1 cut(s) | 543 |
| SinI | GGWCC | 2 cut(s) | 455, 1375 |
| SmaI | CCCGGG | 1 cut(s) | 1073 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 699, 1695, 1898 |
| SmlI | CTYRAG | 4 cut(s) | 619, 659, 1363, 1430 |
| SmoI | CTYRAG | 4 cut(s) | 619, 659, 1363, 1430 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 882 |
| Sse9I | AATT | 8 cut(s) | 305, 941, 1050, 1540, 1658, 1677, 1706, 1831 |
| SsiI | CCGC | 6 cut(s) | 87, 90, 901, 1128, 1437, 1702 |
| SspDI | GGCGCC | 1 cut(s) | 541 |
| SspMI | CTAG | 4 cut(s) | 114, 647, 1325, 1394 |
| StyD4I | CCNGG | 6 cut(s) | 299, 457, 1015, 1070, 1071, 1836 |
| StyI | CCWWGG | 3 cut(s) | 333, 964, 1404 |
| TaaI | ACNGT | 5 cut(s) | 219, 717, 1499, 1728, 1872 |
| TaiI | ACGT | 3 cut(s) | 762, 1264, 1743 |
| TaqI | TCGA | 7 cut(s) | 52, 152, 276, 685, 732, 828, 1929 |
| TasI | AATT | 8 cut(s) | 305, 941, 1050, 1540, 1658, 1677, 1706, 1831 |
| TauI | GCSGC | 1 cut(s) | 92 |
| TfiI | GAWTC | 4 cut(s) | 437, 691, 825, 1926 |
| TscAI | CASTG | 3 cut(s) | 873, 1504, 1591 |
| TseFI | GTSAC | 2 cut(s) | 582, 701 |
| TseI | GCWGC | 4 cut(s) | 107, 1225, 1228, 1253 |
| Tsp45I | GTSAC | 2 cut(s) | 582, 701 |
| TspDTI | ATGAA | 4 cut(s) | 422, 972, 1413, 1706 |
| TspGWI | ACGGA | 1 cut(s) | 703 |
| TspMI | CCCGGG | 1 cut(s) | 1071 |
| TspRI | CASTG | 3 cut(s) | 873, 1504, 1591 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 392 |
| Vha464I | CTTAAG | 1 cut(s) | 619 |
| VpaK11BI | GGWCC | 2 cut(s) | 455, 1375 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1178 |
| XapI | RAATTY | 2 cut(s) | 305, 941 |
| XbaI | TCTAGA | 1 cut(s) | 113 |
| XceI | RCATGY | 1 cut(s) | 611 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1316 |
| XmaI | CCCGGG | 1 cut(s) | 1071 |
| XmiI | GTMKAC | 1 cut(s) | 52 |
| XmnI | GAANNNNTTC | 2 cut(s) | 681, 1910 |
| XspI | CTAG | 4 cut(s) | 114, 647, 1325, 1394 |
| Zsp2I | ATGCAT | 2 cut(s) | 609, 910 |