Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0001528

Physical Location & Seq

Genomic Coordinates Reverse (-)

36385509 .. 36387285

Gene Structure 1777 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00243930.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1593 bp

ATGGCGATTATCAGTGCAACAATGCCATGGCTTTATGGACCAAAAGGAAAGGACTCAAAGGAGGGCAATGAGTTTAAAGACGCTGAGTCTATTGGGCACGCCATAATGCAAAACCTTCCACAATCCGAAATCGTAGAATCATGCTCTGTGGCAAGGCCTGGATTTGTGAATATTATATTGTCAAAGAGTTGGATGGCTAAGACTCTACAGAAGATGCTTGTTGATGGCATTGAAACATGGACGGCGCAGCTGCAGATGAAAATGGTTGTGGTGGACTTTTCATCTCCAAATATAGCAAAAGAGATGCATGTTGGTGATTGGGGAACTCAGTTTGGTATTTTGATCGAGTCCCTTGTGGAAAAGTACCTGGAGTTGGAAGATTTTAGCGGAATAGCCATTGGAGATCTGCAGGTTTTGTATAAAGAAGCCAATGATAGATTTAAACGTGATCCCGAGTTTAAGAAGTCGGCACGAAAGGCAGTGGTTAAGCTTCAGAGTGGGAAGTCTAAGTACCGCAAGGCGTGGGAGCAATTCTGTGAAACTAGCTGGAGAGAATTTCGGAAGATTTATCAACGCCTTGGAGACAATGGAAACGAAACTAAGAGTGATGCAAGTGATACGAAAGAGAAAAGCATGAGTGTTGCAAGTGATGAGGAAGGGAAAATCAAGAGTGCAATGCAAAAGTGCAACTTAGGGCCTCTTCTGATCCCTATTGAAGGTTGTAATGTTCCACTGATTGTTGTGAAGAGTGATGGAGGCTACAACTATGCGACAACCGATTTGGCAGCTCTTTGGTATCGGCTAAATGAAGAGAAAGCTGAGTGGGTTACATATGTTACTGGTAATGAAACATCCCAGAAGGATCACTTGCATAAGATTTTCAAGGCAGCTCAGTGTGCAGGTTGGCTCCCAGCTGATGAAGGCAGGTCATTATATCCAAAAGCTAGCCATGTTGGTTTTGGTCTTGTTTGTGGAGCAGATGCCAAACCCCTCTGGACTCGCTGCGCCAATGTGGTTAGATTAGTTGATTTACTGGATGAAGCGAAGAACTGCAGCAAAGCAGCTCTAATTGAGCGGGGTAAAGCTAAAGGTAAAAAGTGGGATGAAGATGAGCTTGAGCGAACTGCAGAGGCTGTTGGTTATGGTGCTGTTAAGTATGCTGACCTGAAGAATAGTAGATGTAGCAAATACAAATTCGATTTTGATCAGATGCTAAATGATAAGGGAAACACTGCAGTTTACTTGCTCTATGCTCATGCTCGGATCTGTTCTATAATGAGCAAATCTGGCAGAAACATAGAGGAATTGAAAAGAACAGGAGAAATTGTGCTGGACCATGTTGGGGAACGGCAATTGGCGCTTCATTTGCTAAAATTTTCTGAGGCTGTGGAGGAGAGCTGCACCACTCTATTGCCGAATGTTTTGACTGACTACCTCTACAGTTTATCAGAAATCTTCACTAAGTTTTATGAAGACTGCAAGGTTGCTGGGAGTTCACAGGAAACAAGTAGACTGTTGCGTTGTGAAGCAACAGCCTTAGTGATGAGGAAATGTTTTAATCTGCTCGGAATCGAACCTGTTTACAAGCTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004812 GO:0004814 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006420 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0010467 GO:0016070 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0022414 GO:0032501 GO:0032502 GO:0034641 GO:0034645 GO:0034660 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0071704 GO:0090304 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

Arg_tRNA_synt_N DALR_1 tRNA-synt_1d

Protein Analysis

530

Amino Acids

59.54

Weight (kDa)

7.05

Isoelectric Point (pI)

36.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_1d	PF00750	69 - 105	7.1e-07	tRNA synthetases class I (R), catalytic domain
tRNA-synt_1d	PF00750	103 - 401	1.1e-62	tRNA synthetases class I (R), catalytic domain
DALR_1	PF05746	415 - 530	2.5e-28	DALR anticodon binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0001528)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G66530 AT4G26300 AT4G26300 AT4G26300 AT4G26300 AT4G26300
fragaria_vesca	FvH4_1g06400 FvH4_1g06400 FvH4_1g06400 FvH4_1g06400 FvH4_1g06400 FvH4_1g06400
malus_domestica	MD02G1067300.v1.1 MD15G1198200.v1.1 MD15G1199100.v1.1
prunus_persica	Prupe.7G218100_v2.0.a1 Prupe.7G218100_v2.0.a1 Prupe.7G218100_v2.0.a1 Prupe.7G218100_v2.0.a1
pyrus_communis	pycom02g05330 pycom15g17660
rosa_chinensis	RchiOBHm_Chr2g0092141 RchiOBHm_Chr3g0454631 RchiOBHm_Chr3g0454641 RchiOBHm_Chr7g0215671
rosa_laevigata	RLG00000016278 RLG00000025428
rosa_multiflora	Rmu_co8501171.1_g000001 Rmu_sc0000898.1_g000026 Rmu_sc0001792.1_g000032 Rmu_sc0001792.1_g000033 Rmu_sc0013552.1_g000004
rosa_roxburghii	Rroxscaffold_2G00149440 Rroxscaffold_3G00243930
rosa_rugosa	Rorug02G0023000 Rorug02G0023100 Rorug02G0023200 Rorug03G0000700 Rorug03G0000800 Rorug07G0156100
rosa_samantha	Rh2AG068800 Rh2BG068100 Rh2CG069300 Rh2DG067400 Rh3DG062300 Rh7AG292900 Rh7BG285300 Rh7CG313000 Rh7DG296400
rosa_wichuraiana	Rw2G006350 Rw7G024930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	3 cut(s)	400, 890, 915
AccBSI	CCGCTC	1 cut(s)	1075
AccI	GTMKAC	1 cut(s)	1510
AciI	CCGC	3 cut(s)	387, 514, 1075
AclWI	GGATC	4 cut(s)	443, 700, 870, 1271
AcsI	RAATTY	3 cut(s)	554, 1193, 1373
AcuI	CTGAAG	2 cut(s)	476, 1187
AfaI	GTAC	2 cut(s)	365, 512
AfiI	CCNNNNNNNGG	3 cut(s)	373, 716, 1342
AgsI	TTSAA	4 cut(s)	233, 716, 883, 1309
AhdI	GACNNNNNGTC	1 cut(s)	85
AjnI	CCWGG	2 cut(s)	157, 366
Alw26I	GTCTC	1 cut(s)	576
AlwI	GGATC	4 cut(s)	443, 700, 870, 1271
AlwNI	CAGNNNCTG	1 cut(s)	1133
Ama87I	CYCGRG	1 cut(s)	452
AoxI	GGCC	2 cut(s)	155, 695
ApeKI	GCWGC	8 cut(s)	247, 250, 785, 887, 1002, 1053, 1061, 1398
ApoI	RAATTY	3 cut(s)	554, 1193, 1373
ArsI	GACNNNNNNTTYG	2 cut(s)	763, 795
AspLEI	GCGC	3 cut(s)	247, 1007, 1360
AspS9I	GGNCC	3 cut(s)	38, 695, 1333
AsuHPI	GGTGA	1 cut(s)	326
AsuNHI	GCTAGC	1 cut(s)	944
AvaI	CYCGRG	1 cut(s)	452
AvaII	GGWCC	2 cut(s)	38, 1333
BaeGI	GKGCMC	1 cut(s)	99
BarI	GAAGNNNNNNTAC	2 cut(s)	494, 526
BbsI	GAAGAC	1 cut(s)	1479
BbvI	GCAGC	8 cut(s)	237, 259, 797, 899, 989, 1065, 1073, 1385
BccI	CCATC	3 cut(s)	187, 218, 746
BceAI	ACGGC	2 cut(s)	258, 1364
BciT130I	CCWGG	2 cut(s)	159, 368
BclI	TGATCA	1 cut(s)	1204
BcoDI	GTCTC	1 cut(s)	576
BfaI	CTAG	2 cut(s)	543, 945
BfmI	CTRYAG	7 cut(s)	206, 251, 407, 1051, 1125, 1233, 1438
BfoI	RGCGCY	1 cut(s)	1361
BfuAI	ACCTGC	3 cut(s)	400, 890, 915
BglII	AGATCT	1 cut(s)	403
BisI	GCNGC	8 cut(s)	248, 251, 786, 888, 1003, 1054, 1062, 1399
BlsI	GCNGC	8 cut(s)	249, 252, 787, 889, 1004, 1055, 1063, 1400
Bme1390I	CCNGG	2 cut(s)	159, 368
Bme18I	GGWCC	2 cut(s)	38, 1333
BmeRI	GACNNNNNGTC	1 cut(s)	85
BmeT110I	CYCGRG	1 cut(s)	452
BmgT120I	GGNCC	3 cut(s)	38, 695, 1333
BmiI	GGNNCC	1 cut(s)	908
BmrFI	CCNGG	2 cut(s)	159, 368
BmsI	GCATC	5 cut(s)	204, 294, 598, 970, 1200
BmtI	GCTAGC	1 cut(s)	948
BpiI	GAAGAC	1 cut(s)	1479
BpmI	CTGGAG	2 cut(s)	389, 568
BpuEI	CTTGAG	1 cut(s)	1136
BsaBI	GATNNNNATC	1 cut(s)	1203
BsaJI	CCNNGG	2 cut(s)	26, 577
BsaXI	ACNNNNNCTCC	2 cut(s)	1311, 1341
Bsc4I	CCNNNNNNNGG	3 cut(s)	373, 716, 1342
Bse1I	ACTGG	2 cut(s)	844, 1038
Bse3DI	GCAATG	2 cut(s)	73, 681
Bse8I	GATNNNNATC	1 cut(s)	1203
BseBI	CCWGG	2 cut(s)	159, 368
BseDI	CCNNGG	2 cut(s)	26, 577
BseGI	GGATG	4 cut(s)	198, 851, 1042, 1108
BseJI	GATNNNNATC	1 cut(s)	1203
BseLI	CCNNNNNNNGG	3 cut(s)	373, 716, 1342
BseMI	GCAATG	2 cut(s)	73, 681
BseMII	CTCAG	5 cut(s)	75, 341, 810, 905, 1371
BseNI	ACTGG	2 cut(s)	844, 1038
BseRI	GAGGAG	1 cut(s)	1406
BseSI	GKGCMC	1 cut(s)	99
BseXI	GCAGC	8 cut(s)	237, 259, 797, 899, 989, 1065, 1073, 1385
BseYI	CCCAGC	2 cut(s)	910, 1487
BsgI	GTGCAG	2 cut(s)	918, 1384
BshFI	GGCC	2 cut(s)	157, 697
BsiHKCI	CYCGRG	1 cut(s)	452
BslFI	GGGAC	1 cut(s)	334
BslI	CCNNNNNNNGG	3 cut(s)	373, 716, 1342
BsmAI	GTCTC	1 cut(s)	576
BsmFI	GGGAC	1 cut(s)	334
BsnI	GGCC	2 cut(s)	157, 697
BsoBI	CYCGRG	1 cut(s)	452
Bsp1286I	GDGCHC	1 cut(s)	99
Bsp143I	GATC	7 cut(s)	342, 403, 448, 705, 862, 1204, 1263
Bsp19I	CCATGG	1 cut(s)	26
BspACI	CCGC	3 cut(s)	387, 514, 1075
BspANI	GGCC	2 cut(s)	157, 697
BspCNI	CTCAG	5 cut(s)	76, 340, 811, 904, 1372
BspLI	GGNNCC	1 cut(s)	908
BspMAI	CTGCAG	5 cut(s)	255, 411, 1055, 1129, 1237
BspMI	ACCTGC	3 cut(s)	400, 890, 915
BspOI	GCTAGC	1 cut(s)	948
BspPI	GGATC	4 cut(s)	443, 700, 870, 1271
BsrBI	CCGCTC	1 cut(s)	1075
BsrDI	GCAATG	2 cut(s)	73, 681
BsrI	ACTGG	2 cut(s)	844, 1038
BssECI	CCNNGG	2 cut(s)	26, 577
BssMI	GATC	7 cut(s)	342, 403, 448, 705, 862, 1204, 1263
BssT1I	CCWWGG	2 cut(s)	26, 577
Bst2UI	CCWGG	2 cut(s)	159, 368
Bst4CI	ACNGT	2 cut(s)	1442, 1515
Bst6I	CTCTTC	3 cut(s)	705, 740, 804
BstC8I	GCNNGC	2 cut(s)	99, 946
BstDSI	CCRYGG	1 cut(s)	26
BstENI	CCTNNNNNAGG	1 cut(s)	714
BstF5I	GGATG	4 cut(s)	198, 851, 1042, 1108
BstH2I	RGCGCY	1 cut(s)	1361
BstHHI	GCGC	3 cut(s)	247, 1007, 1360
BstKTI	GATC	7 cut(s)	345, 406, 451, 708, 865, 1207, 1266
BstMAI	GTCTC	1 cut(s)	576
BstMBI	GATC	7 cut(s)	342, 403, 448, 705, 862, 1204, 1263
BstMWI	GCNNNNNNNGC	5 cut(s)	476, 896, 1287, 1357, 1366
BstNI	CCWGG	2 cut(s)	159, 368
BstNSI	RCATGY	1 cut(s)	311
BstSCI	CCNGG	2 cut(s)	157, 366
BstSFI	CTRYAG	7 cut(s)	206, 251, 407, 1051, 1125, 1233, 1438
BstSLI	GKGCMC	1 cut(s)	99
BstV1I	GCAGC	8 cut(s)	237, 259, 797, 899, 989, 1065, 1073, 1385
BstV2I	GAAGAC	1 cut(s)	1479
BstX2I	RGATCY	2 cut(s)	403, 1263
BstYI	RGATCY	2 cut(s)	403, 1263
BsuRI	GGCC	2 cut(s)	157, 697
BtgI	CCRYGG	1 cut(s)	26
BtsCI	GGATG	4 cut(s)	198, 851, 1042, 1108
BtsI	GCAGTG	2 cut(s)	486, 1230
BtsIMutI	CAGTG	5 cut(s)	19, 486, 731, 899, 1230
BveI	ACCTGC	3 cut(s)	400, 890, 915
Cac8I	GCNNGC	2 cut(s)	99, 946
CaiI	CAGNNNCTG	1 cut(s)	1133
CfoI	GCGC	3 cut(s)	247, 1007, 1360
Cfr13I	GGNCC	3 cut(s)	38, 695, 1333
CseI	GACGC	1 cut(s)	89
Csp6I	GTAC	2 cut(s)	364, 511
CviAII	CATG	8 cut(s)	27, 141, 237, 308, 634, 950, 1256, 1337
CviQI	GTAC	2 cut(s)	364, 511
DpnI	GATC	7 cut(s)	344, 405, 450, 707, 864, 1206, 1265
DpnII	GATC	7 cut(s)	342, 403, 448, 705, 862, 1204, 1263
DraI	TTTAAA	2 cut(s)	76, 442
DriI	GACNNNNNGTC	1 cut(s)	85
Eam1104I	CTCTTC	3 cut(s)	705, 740, 804
Eam1105I	GACNNNNNGTC	1 cut(s)	85
EarI	CTCTTC	3 cut(s)	705, 740, 804
Eco130I	CCWWGG	2 cut(s)	26, 577
Eco147I	AGGCCT	1 cut(s)	157
Eco47I	GGWCC	2 cut(s)	38, 1333
Eco57I	CTGAAG	2 cut(s)	476, 1187
Eco88I	CYCGRG	1 cut(s)	452
EcoNI	CCTNNNNNAGG	1 cut(s)	714
EcoO109I	RGGNCCY	1 cut(s)	695
EcoRII	CCWGG	2 cut(s)	157, 366
EcoT14I	CCWWGG	2 cut(s)	26, 577
EcoT22I	ATGCAT	1 cut(s)	309
ErhI	CCWWGG	2 cut(s)	26, 577
FaeI	CATG	8 cut(s)	30, 144, 240, 311, 637, 953, 1259, 1340
FalI	AAGNNNNNCTT	6 cut(s)	674, 706, 851, 883, 1098, 1130
FaqI	GGGAC	1 cut(s)	334
FatI	CATG	8 cut(s)	26, 140, 236, 307, 633, 949, 1255, 1336
FauI	CCCGC	1 cut(s)	1068
FauNDI	CATATG	1 cut(s)	832
FbaI	TGATCA	1 cut(s)	1204
FblI	GTMKAC	1 cut(s)	1510
Fnu4HI	GCNGC	8 cut(s)	248, 251, 786, 888, 1003, 1054, 1062, 1399
FokI	GGATG	4 cut(s)	205, 838, 1049, 1115
Fsp4HI	GCNGC	8 cut(s)	248, 251, 786, 888, 1003, 1054, 1062, 1399
FspBI	CTAG	2 cut(s)	543, 945
GlaI	GCGC	3 cut(s)	246, 1006, 1359
GluI	GCNGC	8 cut(s)	248, 251, 786, 888, 1003, 1054, 1062, 1399
GsaI	CCCAGC	2 cut(s)	914, 1491
GsuI	CTGGAG	2 cut(s)	389, 568
HaeII	RGCGCY	1 cut(s)	1361
HaeIII	GGCC	2 cut(s)	157, 697
HgaI	GACGC	1 cut(s)	89
HhaI	GCGC	3 cut(s)	247, 1007, 1360
Hin1II	CATG	8 cut(s)	30, 144, 240, 311, 637, 953, 1259, 1340
Hin6I	GCGC	3 cut(s)	245, 1005, 1358
HinP1I	GCGC	3 cut(s)	245, 1005, 1358
HindIII	AAGCTT	1 cut(s)	488
HinfI	GANTC	7 cut(s)	53, 86, 137, 202, 347, 997, 1569
HphI	GGTGA	1 cut(s)	326
Hpy166II	GTNNAC	5 cut(s)	274, 1240, 1496, 1511, 1582
Hpy188I	TCNGA	9 cut(s)	127, 495, 561, 705, 1209, 1263, 1381, 1450, 1568
Hpy188III	TCNNGA	3 cut(s)	452, 667, 994
Hpy8I	GTNNAC	5 cut(s)	274, 1240, 1496, 1511, 1582
HpyAV	CCTTC	5 cut(s)	125, 650, 710, 853, 914
HpyCH4III	ACNGT	2 cut(s)	1442, 1515
HpyCH4IV	ACGT	1 cut(s)	445
HpyF10VI	GCNNNNNNNGC	5 cut(s)	476, 896, 1287, 1357, 1366
HpySE526I	ACGT	1 cut(s)	445
Hsp92II	CATG	8 cut(s)	30, 144, 240, 311, 637, 953, 1259, 1340
HspAI	GCGC	3 cut(s)	245, 1005, 1358
Ksp22I	TGATCA	1 cut(s)	1204
Kzo9I	GATC	7 cut(s)	342, 403, 448, 705, 862, 1204, 1263
LmnI	GCTCC	3 cut(s)	526, 912, 974
Lsp1109I	GCAGC	8 cut(s)	237, 259, 797, 899, 989, 1065, 1073, 1385
LweI	GCATC	5 cut(s)	204, 294, 598, 970, 1200
MaeI	CTAG	2 cut(s)	543, 945
MaeII	ACGT	1 cut(s)	445
MaeIII	GTNAC	2 cut(s)	826, 835
MalI	GATC	7 cut(s)	344, 405, 450, 707, 864, 1206, 1265
MbiI	CCGCTC	1 cut(s)	1075
MboI	GATC	7 cut(s)	342, 403, 448, 705, 862, 1204, 1263
MfeI	CAATTG	1 cut(s)	1352
MflI	RGATCY	2 cut(s)	403, 1263
MhlI	GDGCHC	1 cut(s)	99
MluCI	AATT	8 cut(s)	530, 554, 1068, 1193, 1304, 1323, 1352, 1373
MlyI	GAGTC	5 cut(s)	47, 95, 196, 356, 991
MmeI	TCCRAC	2 cut(s)	170, 354
Mph1103I	ATGCAT	1 cut(s)	309
MseI	TTAA	6 cut(s)	75, 441, 459, 486, 1152, 1557
MslI	CAYNNNNRTG	1 cut(s)	312
MspA1I	CMGCKG	2 cut(s)	250, 914
MspR9I	CCNGG	2 cut(s)	159, 368
MunI	CAATTG	1 cut(s)	1352
MvaI	CCWGG	2 cut(s)	159, 368
MwoI	GCNNNNNNNGC	5 cut(s)	476, 896, 1287, 1357, 1366
NcoI	CCATGG	1 cut(s)	26
NdeI	CATATG	1 cut(s)	832
NdeII	GATC	7 cut(s)	342, 403, 448, 705, 862, 1204, 1263
NheI	GCTAGC	1 cut(s)	944
NlaIII	CATG	8 cut(s)	30, 144, 240, 311, 637, 953, 1259, 1340
NlaIV	GGNNCC	1 cut(s)	908
NsiI	ATGCAT	1 cut(s)	309
NspI	RCATGY	1 cut(s)	311
PceI	AGGCCT	1 cut(s)	157
PfeI	GAWTC	2 cut(s)	137, 1569
PkrI	GCNGC	8 cut(s)	249, 252, 787, 889, 1004, 1055, 1063, 1400
PleI	GAGTC	5 cut(s)	47, 94, 196, 355, 991
PpsI	GAGTC	5 cut(s)	47, 94, 196, 355, 991
Psp6I	CCWGG	2 cut(s)	157, 366
PspFI	CCCAGC	2 cut(s)	910, 1487
PspGI	CCWGG	2 cut(s)	157, 366
PspN4I	GGNNCC	1 cut(s)	908
PspPI	GGNCC	3 cut(s)	38, 695, 1333
PstI	CTGCAG	5 cut(s)	255, 411, 1055, 1129, 1237
PstNI	CAGNNNCTG	1 cut(s)	1133
PsuI	RGATCY	2 cut(s)	403, 1263
PvuII	CAGCTG	2 cut(s)	250, 914
RsaI	GTAC	2 cut(s)	365, 512
RsaNI	GTAC	2 cut(s)	364, 511
RseI	CAYNNNNRTG	1 cut(s)	312
SaqAI	TTAA	6 cut(s)	75, 441, 459, 486, 1152, 1557
SatI	GCNGC	8 cut(s)	248, 251, 786, 888, 1003, 1054, 1062, 1399
Sau3AI	GATC	7 cut(s)	342, 403, 448, 705, 862, 1204, 1263
Sau96I	GGNCC	3 cut(s)	38, 695, 1333
SchI	GAGTC	5 cut(s)	47, 95, 196, 356, 991
ScrFI	CCNGG	2 cut(s)	159, 368
SduI	GDGCHC	1 cut(s)	99
SfaNI	GCATC	5 cut(s)	204, 294, 598, 970, 1200
SfcI	CTRYAG	7 cut(s)	206, 251, 407, 1051, 1125, 1233, 1438
SinI	GGWCC	2 cut(s)	38, 1333
SmiMI	CAYNNNNRTG	1 cut(s)	312
SmlI	CTYRAG	1 cut(s)	1115
SmoI	CTYRAG	1 cut(s)	1115
Sse9I	AATT	8 cut(s)	530, 554, 1068, 1193, 1304, 1323, 1352, 1373
SseBI	AGGCCT	1 cut(s)	157
SsiI	CCGC	3 cut(s)	387, 514, 1075
SspI	AATATT	1 cut(s)	172
SspMI	CTAG	2 cut(s)	543, 945
StuI	AGGCCT	1 cut(s)	157
StyD4I	CCNGG	2 cut(s)	157, 366
StyI	CCWWGG	2 cut(s)	26, 577
TaaI	ACNGT	2 cut(s)	1442, 1515
TaiI	ACGT	1 cut(s)	448
TaqI	TCGA	3 cut(s)	345, 1197, 1572
TasI	AATT	8 cut(s)	530, 554, 1068, 1193, 1304, 1323, 1352, 1373
TfiI	GAWTC	2 cut(s)	137, 1569
Tru1I	TTAA	6 cut(s)	75, 441, 459, 486, 1152, 1557
Tru9I	TTAA	6 cut(s)	75, 441, 459, 486, 1152, 1557
TscAI	CASTG	5 cut(s)	19, 486, 738, 899, 1237
TseI	GCWGC	8 cut(s)	247, 250, 785, 887, 1002, 1053, 1061, 1398
TspDTI	ATGAA	9 cut(s)	270, 272, 822, 861, 933, 1053, 1119, 1352, 1485
TspRI	CASTG	5 cut(s)	19, 486, 738, 899, 1237
VpaK11BI	GGWCC	2 cut(s)	38, 1333
XagI	CCTNNNNNAGG	1 cut(s)	714
XapI	RAATTY	3 cut(s)	554, 1193, 1373
XceI	RCATGY	1 cut(s)	311
XcmI	CCANNNNNNNNNTGG	1 cut(s)	956
XmiI	GTMKAC	1 cut(s)	1510
XspI	CTAG	2 cut(s)	543, 945
Zsp2I	ATGCAT	1 cut(s)	309

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.