Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr2

Orthogroup ID

OG0001528

Physical Location & Seq

Genomic Coordinates Forward (+)

3564974 .. 3571130

Gene Structure 6157 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom02g05330.7

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2163 bp

ATGAACATTGTACTTGGGCTACAAGCATACAAAAACACATGTGAACTTGGGCTTTTTCTAAGATTGCCGTGTACTGCAAGTCCAAAATTTCAAAGTCCACTCACCAGTCCAAACATATTTGCTTGTCAAAGCCCTTCGACGTGTGGGACCCCTATCCACTTTTGGCTGACATGGCATCGTCCCATTGACGAATATATAAATAGAGAAAAATTTCGAGGGAAAACAGAAATAAAAAGAGAAGAGAGTAACATGCTAGGTTCCCACTTAAACCTGATAACGCCGATTTCTCCTTCCCTCTGTCGACTCTCATCCCTCCACCCCTCTCCTTCCCTCCCCGCCGCCGACTTGTTGAAGGCGGCATCTAGGAACCTTATTTTTGCAGCAAAGTCCCACTCAGTATCCACAATGGCGACTGAAGAAGAGAATGTGGGAAATACAAAGCGACAGTTAGCGAAATTGTTTGAAGTCTCGCTCAGAAGAATAGTCCCCGATGAGCCAGATGTTGAGCCAGTGATTGCTGCTTGCACTGGGAAGTTTGGCGATTATCAATGTAACAATGCCATGGGTCTATGGTCTAAAGTAAAAGGAAAGGGCAGTGAGTTTAGGGGTCCTCCATCTGTGGGACAGGCGATCATGAAAAATCTTCCTGATTCAGAGATCATAGAATCGTGTTCTGTAGCAGGACCTGGATTTGTGAATGTTGCCTTGTCAAAGAAATGGTTGGCTAAGAGCATACAAAAGATGCTTACTGATGGCATCGATAAATGGGCGCCACAGCTTCCGATCAAAAGGGCTGTGGTTGACTTTTCCTCACCTAATATAGCTAAAGAAATGCATGTTGGCCACTTAAGGTCAACTATTATTGGGGACACACTAGCTCGTATTCTGGAGTTTTCAAATGTTGAAGTTCTTCGACGAAATCATGTTGGTGACTGGGGGACACAGTTTGGTATGTTGATTGAGTTTCTTTTTGAAAAGTTCCCAAACATAGAAGATGTTAATGAAACGGCCATTGGTGATCTGCAGTCTATAAAGCATCAAAACAGAGATTCGATGATCGATCCTGTTTTTAAGGAGAGAGCACACCGGGCAGTGGTTTCCCTTCAGGGTGGCGAACCGAGGTACCGAAATGCATGGCTGAAAATCTGTGAAATCAGCCGTAAAGAATTTCACAAGGTCTATGAACGCCTTGGAGTTCATTTAGAGGAAAAGGGTGAGAGTTTTTATAACCCATATATTCCTGGGGTTTTAAAAGAATTGAGTGATAAAGGGTTAATTGAGGACAGCCAGGGTGCTCGTGTAATCTTTCTGGAAGGGTTTAACATCCCCCTTATTGTCGTCAAGAGCGATGGTGGTTTCAACTATGCTTCAACTGATTTAGCAGCTCTTTGGTATCGCCTGAATGAGGAGAAAGCTGATTGGATCATATATGTTACCGATGTTGGCCAGCAGCAGCATTTTAATATGTTTTTCGAGGCAGCTAAACGTGCAGGTTGGCTCCCAACTGATGGCGCCTTGAAACCAAAAGTTACCCATGTTGGTTTTGGTCTAGTTCTTGGAGAAGATGGAAAACGCTTTCGCACTCGCTCTAGTGAGGTGGTCCGATTGGTTGATTTGCTTGATGAAGCCAAGGAACGTAGCAAAAAGGTTCTTGTTGAACGTGGCTGGACAGAGCAGGAGCTTAATCAAATTGCAGAGGCTGTTGGTTACGGTGCTGTTAAGTATGCTGATCTGAAGAACAACAGATTGACCAATTACACGTTTGATTTTGATCAAATGCTAAGTGATAAGGGAAACACTGCTGTTTACTTGCTCTACGCTCATGCTCGGATCTGTTCCATCATAAGGAAATCTGGCAAAAACATAGAGGAATTGAAAAAAACGGGGGAAATTGTGTTGGATCATGACGGTGAGCGTGAATTGGGGCTTCATTTGCTACAGTTTTCAGAGAACGTGGAGGAGACCTGCACCAATCTATTGCCGAATGTTCTGTGCGAATACCTATACAATTTATCAGAAGTCTTCACCAAAAAATTCTACTCCAATTGCCAGGTTGTCGGGTCTCCTGAGGAAACGAGCAGACTCTTGCTTTGTGAAGCAACAGCCGTTGTGATGAGGAAATGTTTTAATCTTCTTGGAATCGAACCGGTTTACAAGATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004812 GO:0004814 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006420 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0010467 GO:0016070 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0022414 GO:0032501 GO:0032502 GO:0034641 GO:0034645 GO:0034660 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0071704 GO:0090304 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

Arg_tRNA_synt_N DALR_1 tRNA-synt_1d

Protein Analysis

721

Amino Acids

81.0

Weight (kDa)

6.99

Isoelectric Point (pI)

48.52

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001528)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G66530 AT4G26300 AT4G26300 AT4G26300 AT4G26300 AT4G26300
fragaria_vesca	FvH4_1g06400 FvH4_1g06400 FvH4_1g06400 FvH4_1g06400 FvH4_1g06400 FvH4_1g06400
malus_domestica	MD02G1067300.v1.1 MD15G1198200.v1.1 MD15G1199100.v1.1
prunus_persica	Prupe.7G218100_v2.0.a1 Prupe.7G218100_v2.0.a1 Prupe.7G218100_v2.0.a1 Prupe.7G218100_v2.0.a1
pyrus_communis	pycom02g05330 pycom15g17660
rosa_chinensis	RchiOBHm_Chr2g0092141 RchiOBHm_Chr3g0454631 RchiOBHm_Chr3g0454641 RchiOBHm_Chr7g0215671
rosa_laevigata	RLG00000016278 RLG00000025428
rosa_multiflora	Rmu_co8501171.1_g000001 Rmu_sc0000898.1_g000026 Rmu_sc0001792.1_g000032 Rmu_sc0001792.1_g000033 Rmu_sc0013552.1_g000004
rosa_roxburghii	Rroxscaffold_2G00149440 Rroxscaffold_3G00243930
rosa_rugosa	Rorug02G0023000 Rorug02G0023100 Rorug02G0023200 Rorug03G0000700 Rorug03G0000800 Rorug07G0156100
rosa_samantha	Rh2AG068800 Rh2BG068100 Rh2CG069300 Rh2DG067400 Rh3DG062300 Rh7AG292900 Rh7BG285300 Rh7CG313000 Rh7DG296400
rosa_wichuraiana	Rw2G006350 Rw7G024930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1227
Acc36I	ACCTGC	2 cut(s)	1481, 1973
Acc65I	GGTACC	1 cut(s)	1122
AccB1I	GGYRCC	3 cut(s)	769, 1122, 1511
AccB7I	CCANNNNNTGG	1 cut(s)	1508
AccI	GTMKAC	1 cut(s)	301
AciI	CCGC	3 cut(s)	336, 339, 356
AclWI	GGATC	4 cut(s)	1055, 1430, 1838, 1908
AcoI	YGGCCR	3 cut(s)	841, 1008, 1444
AcsI	RAATTY	4 cut(s)	86, 209, 1166, 2033
AcuI	CTGAAG	3 cut(s)	435, 1088, 1754
AcyI	GRCGYC	2 cut(s)	770, 1512
AfaI	GTAC	3 cut(s)	12, 73, 1124
AfiI	CCNNNNNNNGG	5 cut(s)	620, 1093, 1405, 1508, 1845
AflII	CTTAAG	1 cut(s)	847
AflIII	ACRYGT	3 cut(s)	38, 140, 1758
AgeI	ACCGGT	1 cut(s)	2146
AjiI	CACGTC	1 cut(s)	141
AjnI	CCWGG	4 cut(s)	685, 1240, 1287, 2049
AjuI	GAANNNNNNNTTGG	4 cut(s)	822, 854, 996, 1028
AluBI	AGCT	7 cut(s)	778, 824, 878, 1385, 1415, 1481, 1681
AluI	AGCT	7 cut(s)	778, 824, 878, 1385, 1415, 1481, 1681
Alw21I	GWGCWC	2 cut(s)	1084, 1297
Alw26I	GTCTC	3 cut(s)	472, 1955, 2067
AlwI	GGATC	4 cut(s)	1055, 1430, 1838, 1908
AlwNI	CAGNNNCTG	2 cut(s)	686, 1700
AoxI	GGCC	3 cut(s)	841, 1008, 1444
ApeKI	GCWGC	6 cut(s)	380, 518, 1382, 1450, 1453, 1478
ApoI	RAATTY	4 cut(s)	86, 209, 1166, 2033
ArsI	GACNNNNNNTTYG	2 cut(s)	2073, 2105
AsiGI	ACCGGT	1 cut(s)	2146
Asp700I	GAANNNNTTC	2 cut(s)	210, 909
Asp718I	GGTACC	1 cut(s)	1122
AspLEI	GCGC	2 cut(s)	772, 1514
AspS9I	GGNCC	4 cut(s)	147, 608, 683, 1600
AsuC2I	CCSGG	1 cut(s)	1088
AsuHPI	GGTGA	7 cut(s)	94, 804, 941, 1028, 1226, 1922, 2017
AvaII	GGWCC	4 cut(s)	147, 608, 683, 1600
AxyI	CCTNAGG	1 cut(s)	2067
BaeI	ACNNNNGTAYC	2 cut(s)	1106, 1139
BalI	TGGCCA	2 cut(s)	843, 1446
BanI	GGYRCC	3 cut(s)	769, 1122, 1511
BauI	CACGAG	1 cut(s)	1296
BbsI	GAAGAC	1 cut(s)	2014
Bbv12I	GWGCWC	2 cut(s)	1084, 1297
BbvI	GCAGC	6 cut(s)	392, 505, 1394, 1462, 1465, 1490
BccI	CCATC	6 cut(s)	622, 746, 1343, 1502, 1559, 1847
BceAI	ACGGC	4 cut(s)	52, 1023, 1143, 2090
BciT130I	CCWGG	4 cut(s)	687, 1242, 1289, 2051
BciVI	GTATCC	1 cut(s)	409
BclI	TGATCA	1 cut(s)	1771
BcnI	CCSGG	1 cut(s)	1088
BcoDI	GTCTC	3 cut(s)	472, 1955, 2067
BfaI	CTAG	5 cut(s)	254, 363, 875, 1550, 1590
BfmI	CTRYAG	3 cut(s)	675, 1022, 1937
BfoI	RGCGCY	2 cut(s)	773, 1515
BfrI	CTTAAG	1 cut(s)	847
BfuAI	ACCTGC	2 cut(s)	1481, 1973
BfuI	GTATCC	1 cut(s)	409
BisI	GCNGC	8 cut(s)	339, 357, 381, 519, 1383, 1451, 1454, 1479
BlsI	GCNGC	8 cut(s)	340, 358, 382, 520, 1384, 1452, 1455, 1480
Bme1390I	CCNGG	5 cut(s)	687, 1088, 1242, 1289, 2051
Bme18I	GGWCC	4 cut(s)	147, 608, 683, 1600
BmgBI	CACGTC	1 cut(s)	141
BmgT120I	GGNCC	4 cut(s)	147, 608, 683, 1600
BmiI	GGNNCC	9 cut(s)	148, 149, 259, 368, 609, 771, 1124, 1499, 1513
BmrFI	CCNGG	5 cut(s)	687, 1088, 1242, 1289, 2051
BmrI	ACTGGG	2 cut(s)	537, 943
BmsI	GCATC	5 cut(s)	184, 368, 732, 765, 1045
BmuI	ACTGGG	2 cut(s)	537, 943
BpiI	GAAGAC	1 cut(s)	2014
BpmI	CTGGAG	1 cut(s)	908
BpuMI	CCSGG	1 cut(s)	1088
Bsa29I	ATCGAT	2 cut(s)	759, 1059
BsaBI	GATNNNNATC	1 cut(s)	1770
BsaHI	GRCGYC	2 cut(s)	770, 1512
BsaI	GGTCTC	2 cut(s)	1955, 2067
BsaJI	CCNNGG	6 cut(s)	561, 1118, 1189, 1241, 1288, 1629
BsaWI	WCCGGW	1 cut(s)	2146
Bsc4I	CCNNNNNNNGG	5 cut(s)	620, 1093, 1405, 1508, 1845
Bse118I	RCCGGY	1 cut(s)	2146
Bse1I	ACTGG	4 cut(s)	105, 509, 532, 938
Bse21I	CCTNAGG	1 cut(s)	2067
Bse8I	GATNNNNATC	1 cut(s)	1770
BseBI	CCWGG	4 cut(s)	687, 1242, 1289, 2051
BseCI	ATCGAT	2 cut(s)	759, 1059
BseDI	CCNNGG	6 cut(s)	561, 1118, 1189, 1241, 1288, 1629
BseGI	GGATG	2 cut(s)	308, 1323
BseJI	GATNNNNATC	1 cut(s)	1770
BseLI	CCNNNNNNNGG	5 cut(s)	620, 1093, 1405, 1508, 1845
BseMII	CTCAG	3 cut(s)	408, 487, 2058
BseNI	ACTGG	4 cut(s)	105, 509, 532, 938
BseRI	GAGGAG	2 cut(s)	1421, 1973
BseXI	GCAGC	6 cut(s)	392, 505, 1394, 1462, 1465, 1490
BsgI	GTGCAG	2 cut(s)	1509, 1951
BshFI	GGCC	3 cut(s)	843, 1010, 1446
BshNI	GGYRCC	3 cut(s)	769, 1122, 1511
BshTI	ACCGGT	1 cut(s)	2146
BshVI	ATCGAT	2 cut(s)	759, 1059
BsiHKAI	GWGCWC	2 cut(s)	1084, 1297
BsiSI	CCGG	2 cut(s)	1087, 2147
BslFI	GGGAC	7 cut(s)	160, 165, 373, 470, 636, 881, 952
BslI	CCNNNNNNNGG	5 cut(s)	620, 1093, 1405, 1508, 1845
BsmAI	GTCTC	3 cut(s)	472, 1955, 2067
BsmFI	GGGAC	7 cut(s)	160, 165, 373, 470, 636, 881, 952
BsnI	GGCC	3 cut(s)	843, 1010, 1446
Bso31I	GGTCTC	2 cut(s)	1955, 2067
Bsp1286I	GDGCHC	2 cut(s)	1084, 1297
Bsp19I	CCATGG	1 cut(s)	561
BspACI	CCGC	3 cut(s)	336, 339, 356
BspANI	GGCC	3 cut(s)	843, 1010, 1446
BspCNI	CTCAG	3 cut(s)	407, 486, 2059
BspDI	ATCGAT	2 cut(s)	759, 1059
BspHI	TCATGA	2 cut(s)	633, 1903
BspLI	GGNNCC	9 cut(s)	148, 149, 259, 368, 609, 771, 1124, 1499, 1513
BspMAI	CTGCAG	1 cut(s)	1026
BspMI	ACCTGC	2 cut(s)	1481, 1973
BspPI	GGATC	4 cut(s)	1055, 1430, 1838, 1908
BspT107I	GGYRCC	3 cut(s)	769, 1122, 1511
BspTI	CTTAAG	1 cut(s)	847
BspTNI	GGTCTC	2 cut(s)	1955, 2067
BsrFI	RCCGGY	1 cut(s)	2146
BsrI	ACTGG	4 cut(s)	105, 509, 532, 938
BssAI	RCCGGY	1 cut(s)	2146
BssECI	CCNNGG	6 cut(s)	561, 1118, 1189, 1241, 1288, 1629
BssNI	GRCGYC	2 cut(s)	770, 1512
BssSI	CACGAG	1 cut(s)	1296
BssT1I	CCWWGG	3 cut(s)	561, 1189, 1629
Bst2BI	CACGAG	1 cut(s)	1296
Bst2UI	CCWGG	4 cut(s)	687, 1242, 1289, 2051
Bst4CI	ACNGT	5 cut(s)	447, 945, 1712, 1910, 1941
Bst6I	CTCTTC	2 cut(s)	234, 414
BstACI	GRCGYC	2 cut(s)	770, 1512
BstAFI	CTTAAG	1 cut(s)	847
BstC8I	GCNNGC	2 cut(s)	523, 1448
BstDEI	CTNAG	6 cut(s)	59, 394, 473, 726, 1781, 2067
BstDSI	CCRYGG	1 cut(s)	561
BstENI	CCTNNNNNAGG	1 cut(s)	1403
BstF5I	GGATG	2 cut(s)	308, 1323
BstH2I	RGCGCY	2 cut(s)	773, 1515
BstHHI	GCGC	2 cut(s)	772, 1514
BstMAI	GTCTC	3 cut(s)	472, 1955, 2067
BstMWI	GCNNNNNNNGC	4 cut(s)	172, 1088, 1487, 1933
BstNI	CCWGG	4 cut(s)	687, 1242, 1289, 2051
BstNSI	RCATGY	3 cut(s)	42, 253, 839
BstSCI	CCNGG	5 cut(s)	685, 1086, 1240, 1287, 2049
BstSFI	CTRYAG	3 cut(s)	675, 1022, 1937
BstV1I	GCAGC	6 cut(s)	392, 505, 1394, 1462, 1465, 1490
BstV2I	GAAGAC	1 cut(s)	2014
BstX2I	RGATCY	1 cut(s)	1830
BstYI	RGATCY	1 cut(s)	1830
Bsu15I	ATCGAT	2 cut(s)	759, 1059
Bsu36I	CCTNAGG	1 cut(s)	2067
BsuI	GTATCC	1 cut(s)	409
BsuRI	GGCC	3 cut(s)	843, 1010, 1446
BsuTUI	ATCGAT	2 cut(s)	759, 1059
BtgI	CCRYGG	1 cut(s)	561
BtgZI	GCGATG	1 cut(s)	1362
BtrI	CACGTC	1 cut(s)	141
BtsCI	GGATG	2 cut(s)	308, 1323
BtsI	GCAGTG	3 cut(s)	601, 1098, 1797
BtsIMutI	CAGTG	5 cut(s)	516, 525, 601, 1098, 1797
BveI	ACCTGC	2 cut(s)	1481, 1973
Cac8I	GCNNGC	2 cut(s)	523, 1448
CaiI	CAGNNNCTG	2 cut(s)	686, 1700
CciI	TCATGA	2 cut(s)	633, 1903
CfoI	GCGC	2 cut(s)	772, 1514
Cfr10I	RCCGGY	1 cut(s)	2146
Cfr13I	GGNCC	4 cut(s)	147, 608, 683, 1600
ClaI	ATCGAT	2 cut(s)	759, 1059
Csp6I	GTAC	3 cut(s)	11, 72, 1123
CspAI	ACCGGT	1 cut(s)	2146
CviQI	GTAC	3 cut(s)	11, 72, 1123
DdeI	CTNAG	6 cut(s)	59, 394, 473, 726, 1781, 2067
DinI	GGCGCC	2 cut(s)	771, 1513
DraI	TTTAAA	1 cut(s)	1251
EaeI	YGGCCR	3 cut(s)	841, 1008, 1444
Eam1104I	CTCTTC	2 cut(s)	234, 414
EarI	CTCTTC	2 cut(s)	234, 414
Eco130I	CCWWGG	3 cut(s)	561, 1189, 1629
Eco31I	GGTCTC	2 cut(s)	1955, 2067
Eco47I	GGWCC	4 cut(s)	147, 608, 683, 1600
Eco57I	CTGAAG	3 cut(s)	435, 1088, 1754
Eco81I	CCTNAGG	1 cut(s)	2067
EcoNI	CCTNNNNNAGG	1 cut(s)	1403
EcoO109I	RGGNCCY	3 cut(s)	147, 608, 683
EcoRII	CCWGG	4 cut(s)	685, 1240, 1287, 2049
EcoT14I	CCWWGG	3 cut(s)	561, 1189, 1629
EcoT22I	ATGCAT	2 cut(s)	837, 1135
EgeI	GGCGCC	2 cut(s)	771, 1513
EheI	GGCGCC	2 cut(s)	771, 1513
ErhI	CCWWGG	3 cut(s)	561, 1189, 1629
FaqI	GGGAC	7 cut(s)	160, 165, 373, 470, 636, 881, 952
FauI	CCCGC	1 cut(s)	343
FbaI	TGATCA	1 cut(s)	1771
FblI	GTMKAC	1 cut(s)	301
Fnu4HI	GCNGC	8 cut(s)	339, 357, 381, 519, 1383, 1451, 1454, 1479
FokI	GGATG	2 cut(s)	295, 1310
Fsp4HI	GCNGC	8 cut(s)	339, 357, 381, 519, 1383, 1451, 1454, 1479
FspBI	CTAG	5 cut(s)	254, 363, 875, 1550, 1590
GlaI	GCGC	2 cut(s)	771, 1513
GluI	GCNGC	8 cut(s)	339, 357, 381, 519, 1383, 1451, 1454, 1479
GsuI	CTGGAG	1 cut(s)	908
HaeII	RGCGCY	2 cut(s)	773, 1515
HaeIII	GGCC	3 cut(s)	843, 1010, 1446
HapII	CCGG	2 cut(s)	1087, 2147
HhaI	GCGC	2 cut(s)	772, 1514
Hin1I	GRCGYC	2 cut(s)	770, 1512
Hin6I	GCGC	2 cut(s)	770, 1512
HinP1I	GCGC	2 cut(s)	770, 1512
HincII	GTYRAC	3 cut(s)	302, 802, 855
HindII	GTYRAC	3 cut(s)	302, 802, 855
HinfI	GANTC	6 cut(s)	303, 650, 665, 1049, 2082, 2139
HpaII	CCGG	2 cut(s)	1087, 2147
HphI	GGTGA	7 cut(s)	94, 804, 941, 1028, 1226, 1922, 2017
Hpy166II	GTNNAC	8 cut(s)	44, 72, 98, 302, 802, 855, 1807, 2152
Hpy188I	TCNGA	8 cut(s)	476, 655, 783, 1604, 1734, 1830, 1948, 2017
Hpy188III	TCNNGA	7 cut(s)	634, 647, 887, 1310, 1342, 1904, 2066
Hpy8I	GTNNAC	8 cut(s)	44, 72, 98, 302, 802, 855, 1807, 2152
Hpy99I	CGWCG	2 cut(s)	142, 918
HpyAV	CCTTC	6 cut(s)	144, 300, 336, 346, 1112, 1307
HpyCH4III	ACNGT	5 cut(s)	447, 945, 1712, 1910, 1941
HpyCH4IV	ACGT	6 cut(s)	140, 1486, 1636, 1660, 1760, 1953
HpyCH4V	TGCA	9 cut(s)	77, 380, 525, 835, 1024, 1133, 1490, 1694, 1968
HpyF10VI	GCNNNNNNNGC	4 cut(s)	172, 1088, 1487, 1933
HpyF3I	CTNAG	6 cut(s)	59, 394, 473, 726, 1781, 2067
HpySE526I	ACGT	6 cut(s)	140, 1486, 1636, 1660, 1760, 1953
Hsp92I	GRCGYC	2 cut(s)	770, 1512
HspAI	GCGC	2 cut(s)	770, 1512
KasI	GGCGCC	2 cut(s)	769, 1511
KflI	GGGWCCC	1 cut(s)	147
KpnI	GGTACC	1 cut(s)	1126
Ksp22I	TGATCA	1 cut(s)	1771
LmnI	GCTCC	2 cut(s)	1503, 1678
Lsp1109I	GCAGC	6 cut(s)	392, 505, 1394, 1462, 1465, 1490
LweI	GCATC	5 cut(s)	184, 368, 732, 765, 1045
MaeI	CTAG	5 cut(s)	254, 363, 875, 1550, 1590
MaeII	ACGT	6 cut(s)	140, 1486, 1636, 1660, 1760, 1953
MaeIII	GTNAC	6 cut(s)	245, 551, 929, 1432, 1528, 1706
MfeI	CAATTG	1 cut(s)	2044
MflI	RGATCY	1 cut(s)	1830
MhlI	GDGCHC	2 cut(s)	1084, 1297
MlsI	TGGCCA	2 cut(s)	843, 1446
MluNI	TGGCCA	2 cut(s)	843, 1446
Mly113I	GGCGCC	2 cut(s)	770, 1512
MlyI	GAGTC	2 cut(s)	297, 2076
MmeI	TCCRAC	1 cut(s)	1878
Mox20I	TGGCCA	2 cut(s)	843, 1446
Mph1103I	ATGCAT	2 cut(s)	837, 1135
MroXI	GAANNNNTTC	2 cut(s)	210, 909
MscI	TGGCCA	2 cut(s)	843, 1446
MslI	CAYNNNNRTG	2 cut(s)	927, 1908
Msp20I	TGGCCA	2 cut(s)	843, 1446
MspCI	CTTAAG	1 cut(s)	847
MspI	CCGG	2 cut(s)	1087, 2147
MspR9I	CCNGG	5 cut(s)	687, 1088, 1242, 1289, 2051
MunI	CAATTG	1 cut(s)	2044
MvaI	CCWGG	4 cut(s)	687, 1242, 1289, 2051
MwoI	GCNNNNNNNGC	4 cut(s)	172, 1088, 1487, 1933
NarI	GGCGCC	2 cut(s)	770, 1512
NciI	CCSGG	1 cut(s)	1088
NcoI	CCATGG	1 cut(s)	561
NlaIV	GGNNCC	9 cut(s)	148, 149, 259, 368, 609, 771, 1124, 1499, 1513
NmuCI	GTSAC	1 cut(s)	929
NsiI	ATGCAT	2 cut(s)	837, 1135
NspI	RCATGY	3 cut(s)	42, 253, 839
PagI	TCATGA	2 cut(s)	633, 1903
PciI	ACATGT	1 cut(s)	38
PcsI	WCGNNNNNNNCGW	1 cut(s)	1344
PdmI	GAANNNNTTC	2 cut(s)	210, 909
PfeI	GAWTC	4 cut(s)	650, 665, 1049, 2139
PflMI	CCANNNNNTGG	1 cut(s)	1508
PinAI	ACCGGT	1 cut(s)	2146
PkrI	GCNGC	8 cut(s)	340, 358, 382, 520, 1384, 1452, 1455, 1480
PleI	GAGTC	2 cut(s)	297, 2076
PluTI	GGCGCC	2 cut(s)	773, 1515
PpsI	GAGTC	2 cut(s)	297, 2076
PpuMI	RGGWCCY	3 cut(s)	147, 608, 683
PscI	ACATGT	1 cut(s)	38
PsiI	TTATAA	1 cut(s)	1227
Psp5II	RGGWCCY	3 cut(s)	147, 608, 683
Psp6I	CCWGG	4 cut(s)	685, 1240, 1287, 2049
PspGI	CCWGG	4 cut(s)	685, 1240, 1287, 2049
PspN4I	GGNNCC	9 cut(s)	148, 149, 259, 368, 609, 771, 1124, 1499, 1513
PspPI	GGNCC	4 cut(s)	147, 608, 683, 1600
PspPPI	RGGWCCY	3 cut(s)	147, 608, 683
PstI	CTGCAG	1 cut(s)	1026
PstNI	CAGNNNCTG	2 cut(s)	686, 1700
PsuI	RGATCY	1 cut(s)	1830
RsaI	GTAC	3 cut(s)	12, 73, 1124
RsaNI	GTAC	3 cut(s)	11, 72, 1123
RseI	CAYNNNNRTG	2 cut(s)	927, 1908
SalI	GTCGAC	1 cut(s)	300
SatI	GCNGC	8 cut(s)	339, 357, 381, 519, 1383, 1451, 1454, 1479
Sau96I	GGNCC	4 cut(s)	147, 608, 683, 1600
SchI	GAGTC	2 cut(s)	297, 2076
ScrFI	CCNGG	5 cut(s)	687, 1088, 1242, 1289, 2051
SduI	GDGCHC	2 cut(s)	1084, 1297
SfaNI	GCATC	5 cut(s)	184, 368, 732, 765, 1045
SfcI	CTRYAG	3 cut(s)	675, 1022, 1937
SfoI	GGCGCC	2 cut(s)	771, 1513
SinI	GGWCC	4 cut(s)	147, 608, 683, 1600
SmiMI	CAYNNNNRTG	2 cut(s)	927, 1908
SmlI	CTYRAG	1 cut(s)	847
SmoI	CTYRAG	1 cut(s)	847
SsiI	CCGC	3 cut(s)	336, 339, 356
SspDI	GGCGCC	2 cut(s)	769, 1511
SspMI	CTAG	5 cut(s)	254, 363, 875, 1550, 1590
StyD4I	CCNGG	5 cut(s)	685, 1086, 1240, 1287, 2049
StyI	CCWWGG	3 cut(s)	561, 1189, 1629
TaaI	ACNGT	5 cut(s)	447, 945, 1712, 1910, 1941
TaiI	ACGT	6 cut(s)	143, 1489, 1639, 1663, 1763, 1956
TaqI	TCGA	9 cut(s)	137, 214, 301, 759, 913, 1052, 1059, 1473, 2142
TatI	WGTACW	2 cut(s)	10, 71
TauI	GCSGC	2 cut(s)	341, 359
TfiI	GAWTC	4 cut(s)	650, 665, 1049, 2139
TscAI	CASTG	5 cut(s)	516, 532, 601, 1098, 1804
TseFI	GTSAC	1 cut(s)	929
TseI	GCWGC	6 cut(s)	380, 518, 1382, 1450, 1453, 1478
Tsp45I	GTSAC	1 cut(s)	929
TspDTI	ATGAA	7 cut(s)	17, 650, 1017, 1187, 1197, 1638, 1919
TspRI	CASTG	5 cut(s)	516, 532, 601, 1098, 1804
Van91I	CCANNNNNTGG	1 cut(s)	1508
Vha464I	CTTAAG	1 cut(s)	847
VpaK11BI	GGWCC	4 cut(s)	147, 608, 683, 1600
XagI	CCTNNNNNAGG	1 cut(s)	1403
XapI	RAATTY	4 cut(s)	86, 209, 1166, 2033
XceI	RCATGY	3 cut(s)	42, 253, 839
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1541
XmiI	GTMKAC	1 cut(s)	301
XmnI	GAANNNNTTC	2 cut(s)	210, 909
XspI	CTAG	5 cut(s)	254, 363, 875, 1550, 1590
Zsp2I	ATGCAT	2 cut(s)	837, 1135

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.