Belongs to the class-I aminoacyl-tRNA synthetase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 987, 1119 |
| Acc36I | ACCTGC | 1 cut(s) | 1241 |
| AccB1I | GGYRCC | 2 cut(s) | 241, 526 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1268 |
| AccI | GTMKAC | 2 cut(s) | 558, 1849 |
| AccIII | TCCGGA | 1 cut(s) | 1335 |
| AciI | CCGC | 8 cut(s) | 32, 35, 93, 96, 199, 1004, 1676, 1797 |
| AclWI | GGATC | 5 cut(s) | 521, 815, 828, 1190, 1607 |
| AcoI | YGGCCR | 2 cut(s) | 598, 1204 |
| AcsI | RAATTY | 2 cut(s) | 290, 785 |
| AcuI | CTGAAG | 4 cut(s) | 764, 848, 1461, 1523 |
| AcyI | GRCGYC | 1 cut(s) | 113 |
| AfaI | GTAC | 3 cut(s) | 884, 1277, 1922 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 284, 636, 1072, 1268 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 328 |
| AjnI | CCWGG | 3 cut(s) | 442, 537, 1818 |
| Alw21I | GWGCWC | 2 cut(s) | 844, 1057 |
| Alw26I | GTCTC | 3 cut(s) | 77, 291, 1712 |
| AlwI | GGATC | 5 cut(s) | 521, 815, 828, 1190, 1607 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 443, 1469 |
| Aor13HI | TCCGGA | 1 cut(s) | 1335 |
| AoxI | GGCC | 5 cut(s) | 384, 440, 598, 825, 1204 |
| ApeKI | GCWGC | 5 cut(s) | 167, 211, 848, 1142, 1238 |
| ApoI | RAATTY | 2 cut(s) | 290, 785 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 666, 735 |
| AspLEI | GCGC | 2 cut(s) | 1484, 1696 |
| AspS9I | GGNCC | 2 cut(s) | 9, 440 |
| AsuC2I | CCSGG | 1 cut(s) | 637 |
| AsuHPI | GGTGA | 5 cut(s) | 36, 561, 698, 785, 1691 |
| AsuII | TTCGAA | 1 cut(s) | 729 |
| AvaII | GGWCC | 1 cut(s) | 9 |
| BaeGI | GKGCMC | 1 cut(s) | 529 |
| BalI | TGGCCA | 2 cut(s) | 600, 1206 |
| BamHI | GGATCC | 1 cut(s) | 820 |
| BanI | GGYRCC | 2 cut(s) | 241, 526 |
| BauI | CACGAG | 1 cut(s) | 1056 |
| BbsI | GAAGAC | 1 cut(s) | 1437 |
| Bbv12I | GWGCWC | 2 cut(s) | 844, 1057 |
| BbvI | GCAGC | 5 cut(s) | 179, 223, 835, 1154, 1250 |
| BccI | CCATC | 6 cut(s) | 395, 472, 503, 1074, 1262, 1316 |
| BceAI | ACGGC | 4 cut(s) | 37, 903, 962, 1901 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1729, 1763 |
| BciT130I | CCWGG | 3 cut(s) | 444, 539, 1820 |
| BciVI | GTATCC | 1 cut(s) | 868 |
| BcnI | CCSGG | 1 cut(s) | 637 |
| BcoDI | GTCTC | 3 cut(s) | 77, 291, 1712 |
| BfaI | CTAG | 1 cut(s) | 632 |
| BfmI | CTRYAG | 3 cut(s) | 849, 1461, 1792 |
| BfoI | RGCGCY | 2 cut(s) | 1485, 1697 |
| BfuAI | ACCTGC | 1 cut(s) | 1241 |
| BfuI | GTATCC | 1 cut(s) | 868 |
| BisI | GCNGC | 8 cut(s) | 35, 96, 168, 200, 212, 849, 1143, 1239 |
| BlsI | GCNGC | 8 cut(s) | 36, 97, 169, 201, 213, 850, 1144, 1240 |
| Bme1390I | CCNGG | 4 cut(s) | 444, 539, 637, 1820 |
| Bme18I | GGWCC | 1 cut(s) | 9 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 328 |
| BmgT120I | GGNCC | 2 cut(s) | 9, 440 |
| BmiI | GGNNCC | 5 cut(s) | 10, 243, 264, 528, 822 |
| BmrFI | CCNGG | 4 cut(s) | 444, 539, 637, 1820 |
| BmrI | ACTGGG | 1 cut(s) | 700 |
| BmsI | GCATC | 3 cut(s) | 489, 579, 630 |
| BmuI | ACTGGG | 1 cut(s) | 700 |
| BpiI | GAAGAC | 1 cut(s) | 1437 |
| BpmI | CTGGAG | 2 cut(s) | 192, 521 |
| Bpu14I | TTCGAA | 1 cut(s) | 729 |
| BpuEI | CTTGAG | 3 cut(s) | 665, 1091, 1472 |
| BpuMI | CCSGG | 1 cut(s) | 637 |
| BsaBI | GATNNNNATC | 2 cut(s) | 819, 1539 |
| BsaHI | GRCGYC | 1 cut(s) | 113 |
| BsaJI | CCNNGG | 5 cut(s) | 162, 318, 443, 582, 1027 |
| BsaWI | WCCGGW | 1 cut(s) | 1335 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 168, 198, 519, 549 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 284, 636, 1072, 1268 |
| Bse118I | RCCGGY | 1 cut(s) | 265 |
| Bse1I | ACTGG | 3 cut(s) | 695, 1262, 1439 |
| Bse3DI | GCAATG | 1 cut(s) | 1114 |
| Bse8I | GATNNNNATC | 2 cut(s) | 819, 1539 |
| BseAI | TCCGGA | 1 cut(s) | 1335 |
| BseBI | CCWGG | 3 cut(s) | 444, 539, 1820 |
| BseDI | CCNNGG | 5 cut(s) | 162, 318, 443, 582, 1027 |
| BseGI | GGATG | 5 cut(s) | 116, 483, 645, 754, 1083 |
| BseJI | GATNNNNATC | 2 cut(s) | 819, 1539 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 284, 636, 1072, 1268 |
| BseMI | GCAATG | 1 cut(s) | 1114 |
| BseMII | CTCAG | 1 cut(s) | 1707 |
| BseNI | ACTGG | 3 cut(s) | 695, 1262, 1439 |
| BseRI | GAGGAG | 3 cut(s) | 189, 1181, 1742 |
| BseSI | GKGCMC | 1 cut(s) | 529 |
| BseXI | GCAGC | 5 cut(s) | 179, 223, 835, 1154, 1250 |
| BseYI | CCCAGC | 1 cut(s) | 284 |
| BshFI | GGCC | 5 cut(s) | 386, 442, 600, 827, 1206 |
| BshNI | GGYRCC | 2 cut(s) | 241, 526 |
| BsiHKAI | GWGCWC | 2 cut(s) | 844, 1057 |
| BsiSI | CCGG | 4 cut(s) | 266, 637, 824, 1336 |
| BslFI | GGGAC | 2 cut(s) | 638, 886 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 284, 636, 1072, 1268 |
| BsmAI | GTCTC | 3 cut(s) | 77, 291, 1712 |
| BsmBI | CGTCTC | 1 cut(s) | 77 |
| BsmFI | GGGAC | 2 cut(s) | 638, 886 |
| BsnI | GGCC | 5 cut(s) | 386, 442, 600, 827, 1206 |
| Bsp119I | TTCGAA | 1 cut(s) | 729 |
| Bsp1286I | GDGCHC | 3 cut(s) | 529, 844, 1057 |
| Bsp13I | TCCGGA | 1 cut(s) | 1335 |
| Bsp1407I | TGTACA | 1 cut(s) | 1920 |
| Bsp143I | GATC | 7 cut(s) | 513, 608, 775, 820, 1182, 1498, 1599 |
| Bsp19I | CCATGG | 2 cut(s) | 162, 318 |
| BspACI | CCGC | 8 cut(s) | 32, 35, 93, 96, 199, 1004, 1676, 1797 |
| BspANI | GGCC | 5 cut(s) | 386, 442, 600, 827, 1206 |
| BspCNI | CTCAG | 1 cut(s) | 1708 |
| BspEI | TCCGGA | 1 cut(s) | 1335 |
| BspHI | TCATGA | 1 cut(s) | 1881 |
| BspLI | GGNNCC | 5 cut(s) | 10, 243, 264, 528, 822 |
| BspMAI | CTGCAG | 2 cut(s) | 853, 1465 |
| BspMI | ACCTGC | 1 cut(s) | 1241 |
| BspPI | GGATC | 5 cut(s) | 521, 815, 828, 1190, 1607 |
| BspQI | GCTCTTC | 3 cut(s) | 1097, 1351, 1440 |
| BspT104I | TTCGAA | 1 cut(s) | 729 |
| BspT107I | GGYRCC | 2 cut(s) | 241, 526 |
| BsrDI | GCAATG | 1 cut(s) | 1114 |
| BsrFI | RCCGGY | 1 cut(s) | 265 |
| BsrGI | TGTACA | 1 cut(s) | 1920 |
| BsrI | ACTGG | 3 cut(s) | 695, 1262, 1439 |
| BssAI | RCCGGY | 1 cut(s) | 265 |
| BssECI | CCNNGG | 5 cut(s) | 162, 318, 443, 582, 1027 |
| BssMI | GATC | 7 cut(s) | 513, 608, 775, 820, 1182, 1498, 1599 |
| BssNI | GRCGYC | 1 cut(s) | 113 |
| BssSI | CACGAG | 1 cut(s) | 1056 |
| BssT1I | CCWWGG | 4 cut(s) | 162, 318, 582, 1027 |
| Bst2BI | CACGAG | 1 cut(s) | 1056 |
| Bst2UI | CCWGG | 3 cut(s) | 444, 539, 1820 |
| Bst4CI | ACNGT | 4 cut(s) | 433, 702, 887, 1723 |
| Bst6I | CTCTTC | 3 cut(s) | 1097, 1351, 1440 |
| BstACI | GRCGYC | 1 cut(s) | 113 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 848 |
| BstAUI | TGTACA | 1 cut(s) | 1920 |
| BstBI | TTCGAA | 1 cut(s) | 729 |
| BstC8I | GCNNGC | 4 cut(s) | 148, 1208, 1593, 1735 |
| BstDEI | CTNAG | 4 cut(s) | 141, 333, 483, 1716 |
| BstDSI | CCRYGG | 2 cut(s) | 162, 318 |
| BstF5I | GGATG | 5 cut(s) | 116, 483, 645, 754, 1083 |
| BstH2I | RGCGCY | 2 cut(s) | 1485, 1697 |
| BstHHI | GCGC | 2 cut(s) | 1484, 1696 |
| BstKTI | GATC | 7 cut(s) | 516, 611, 778, 823, 1185, 1501, 1602 |
| BstMAI | GTCTC | 3 cut(s) | 77, 291, 1712 |
| BstMBI | GATC | 7 cut(s) | 513, 608, 775, 820, 1182, 1498, 1599 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 143, 208, 250, 848, 1247, 1588 |
| BstNI | CCWGG | 3 cut(s) | 444, 539, 1820 |
| BstNSI | RCATGY | 4 cut(s) | 596, 712, 1591, 1595 |
| BstSCI | CCNGG | 4 cut(s) | 442, 537, 635, 1818 |
| BstSFI | CTRYAG | 3 cut(s) | 849, 1461, 1792 |
| BstSLI | GKGCMC | 1 cut(s) | 529 |
| BstV1I | GCAGC | 5 cut(s) | 179, 223, 835, 1154, 1250 |
| BstV2I | GAAGAC | 1 cut(s) | 1437 |
| BstX2I | RGATCY | 2 cut(s) | 820, 1599 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 1138, 1826 |
| BstYI | RGATCY | 2 cut(s) | 820, 1599 |
| BsuI | GTATCC | 1 cut(s) | 868 |
| BsuRI | GGCC | 5 cut(s) | 386, 442, 600, 827, 1206 |
| BtgI | CCRYGG | 2 cut(s) | 162, 318 |
| BtsCI | GGATG | 5 cut(s) | 116, 483, 645, 754, 1083 |
| BtsI | GCAGTG | 4 cut(s) | 132, 243, 858, 1566 |
| BtsIMutI | CAGTG | 5 cut(s) | 132, 243, 858, 1131, 1566 |
| BveI | ACCTGC | 1 cut(s) | 1241 |
| Cac8I | GCNNGC | 4 cut(s) | 148, 1208, 1593, 1735 |
| CaiI | CAGNNNCTG | 2 cut(s) | 443, 1469 |
| CciI | TCATGA | 1 cut(s) | 1881 |
| CfoI | GCGC | 2 cut(s) | 1484, 1696 |
| Cfr10I | RCCGGY | 1 cut(s) | 265 |
| Cfr13I | GGNCC | 2 cut(s) | 9, 440 |
| CseI | GACGC | 1 cut(s) | 102 |
| Csp6I | GTAC | 3 cut(s) | 883, 1276, 1921 |
| CviQI | GTAC | 3 cut(s) | 883, 1276, 1921 |
| DdeI | CTNAG | 4 cut(s) | 141, 333, 483, 1716 |
| DpnI | GATC | 7 cut(s) | 515, 610, 777, 822, 1184, 1500, 1601 |
| DpnII | GATC | 7 cut(s) | 513, 608, 775, 820, 1182, 1498, 1599 |
| DriI | GACNNNNNGTC | 1 cut(s) | 328 |
| EaeI | YGGCCR | 2 cut(s) | 598, 1204 |
| Eam1104I | CTCTTC | 3 cut(s) | 1097, 1351, 1440 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 328 |
| EarI | CTCTTC | 3 cut(s) | 1097, 1351, 1440 |
| Eco130I | CCWWGG | 4 cut(s) | 162, 318, 582, 1027 |
| Eco47I | GGWCC | 1 cut(s) | 9 |
| Eco57I | CTGAAG | 4 cut(s) | 764, 848, 1461, 1523 |
| EcoO109I | RGGNCCY | 2 cut(s) | 9, 440 |
| EcoRI | GAATTC | 1 cut(s) | 785 |
| EcoRII | CCWGG | 3 cut(s) | 442, 537, 1818 |
| EcoT14I | CCWWGG | 4 cut(s) | 162, 318, 582, 1027 |
| EcoT22I | ATGCAT | 2 cut(s) | 594, 1593 |
| ErhI | CCWWGG | 4 cut(s) | 162, 318, 582, 1027 |
| Esp3I | CGTCTC | 1 cut(s) | 77 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1434, 1466 |
| FaqI | GGGAC | 2 cut(s) | 638, 886 |
| FauI | CCCGC | 3 cut(s) | 39, 100, 1669 |
| FauNDI | CATATG | 1 cut(s) | 326 |
| FblI | GTMKAC | 2 cut(s) | 558, 1849 |
| Fnu4HI | GCNGC | 8 cut(s) | 35, 96, 168, 200, 212, 849, 1143, 1239 |
| FokI | GGATG | 5 cut(s) | 103, 490, 652, 761, 1070 |
| Fsp4HI | GCNGC | 8 cut(s) | 35, 96, 168, 200, 212, 849, 1143, 1239 |
| FspBI | CTAG | 1 cut(s) | 632 |
| GlaI | GCGC | 2 cut(s) | 1483, 1695 |
| GluI | GCNGC | 8 cut(s) | 35, 96, 168, 200, 212, 849, 1143, 1239 |
| GsaI | CCCAGC | 1 cut(s) | 288 |
| GsuI | CTGGAG | 2 cut(s) | 192, 521 |
| HaeII | RGCGCY | 2 cut(s) | 1485, 1697 |
| HaeIII | GGCC | 5 cut(s) | 386, 442, 600, 827, 1206 |
| HapII | CCGG | 4 cut(s) | 266, 637, 824, 1336 |
| HgaI | GACGC | 1 cut(s) | 102 |
| HhaI | GCGC | 2 cut(s) | 1484, 1696 |
| Hin1I | GRCGYC | 1 cut(s) | 113 |
| Hin6I | GCGC | 2 cut(s) | 1482, 1694 |
| HinP1I | GCGC | 2 cut(s) | 1482, 1694 |
| HincII | GTYRAC | 1 cut(s) | 1020 |
| HindII | GTYRAC | 1 cut(s) | 1020 |
| HindIII | AAGCTT | 2 cut(s) | 125, 979 |
| HinfI | GANTC | 8 cut(s) | 364, 422, 715, 874, 1332, 1339, 1741, 1851 |
| HpaII | CCGG | 4 cut(s) | 266, 637, 824, 1336 |
| HphI | GGTGA | 5 cut(s) | 36, 561, 698, 785, 1691 |
| Hpy166II | GTNNAC | 5 cut(s) | 559, 1020, 1576, 1850, 1921 |
| Hpy188I | TCNGA | 9 cut(s) | 296, 412, 545, 1351, 1503, 1599, 1717, 1786, 1907 |
| Hpy188III | TCNNGA | 5 cut(s) | 361, 782, 1070, 1336, 1882 |
| Hpy8I | GTNNAC | 5 cut(s) | 559, 1020, 1576, 1850, 1921 |
| HpyAV | CCTTC | 5 cut(s) | 57, 103, 288, 838, 872 |
| HpyCH4III | ACNGT | 4 cut(s) | 433, 702, 887, 1723 |
| HpyCH4IV | ACGT | 1 cut(s) | 744 |
| HpyCH4V | TGCA | 7 cut(s) | 137, 429, 592, 851, 1463, 1591, 1737 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 143, 208, 250, 848, 1247, 1588 |
| HpyF3I | CTNAG | 4 cut(s) | 141, 333, 483, 1716 |
| HpySE526I | ACGT | 1 cut(s) | 744 |
| Hsp92I | GRCGYC | 1 cut(s) | 113 |
| HspAI | GCGC | 2 cut(s) | 1482, 1694 |
| Kpn2I | TCCGGA | 1 cut(s) | 1335 |
| Kzo9I | GATC | 7 cut(s) | 513, 608, 775, 820, 1182, 1498, 1599 |
| LguI | GCTCTTC | 3 cut(s) | 1097, 1351, 1440 |
| LmnI | GCTCC | 1 cut(s) | 262 |
| Lsp1109I | GCAGC | 5 cut(s) | 179, 223, 835, 1154, 1250 |
| LweI | GCATC | 3 cut(s) | 489, 579, 630 |
| MaeI | CTAG | 1 cut(s) | 632 |
| MaeII | ACGT | 1 cut(s) | 744 |
| MaeIII | GTNAC | 3 cut(s) | 308, 686, 1192 |
| MalI | GATC | 7 cut(s) | 515, 610, 777, 822, 1184, 1500, 1601 |
| MboI | GATC | 7 cut(s) | 513, 608, 775, 820, 1182, 1498, 1599 |
| MfeI | CAATTG | 1 cut(s) | 1813 |
| MflI | RGATCY | 2 cut(s) | 820, 1599 |
| MhlI | GDGCHC | 3 cut(s) | 529, 844, 1057 |
| MlsI | TGGCCA | 2 cut(s) | 600, 1206 |
| MluNI | TGGCCA | 2 cut(s) | 600, 1206 |
| MlyI | GAGTC | 4 cut(s) | 431, 868, 1333, 1845 |
| MmeI | TCCRAC | 1 cut(s) | 358 |
| Mox20I | TGGCCA | 2 cut(s) | 600, 1206 |
| Mph1103I | ATGCAT | 2 cut(s) | 594, 1593 |
| MroI | TCCGGA | 1 cut(s) | 1335 |
| MroXI | GAANNNNTTC | 2 cut(s) | 666, 735 |
| MscI | TGGCCA | 2 cut(s) | 600, 1206 |
| MseI | TTAA | 6 cut(s) | 339, 453, 1080, 1161, 1221, 1706 |
| MslI | CAYNNNNRTG | 2 cut(s) | 684, 1677 |
| Msp20I | TGGCCA | 2 cut(s) | 600, 1206 |
| MspA1I | CMGCKG | 4 cut(s) | 170, 214, 1004, 1797 |
| MspI | CCGG | 4 cut(s) | 266, 637, 824, 1336 |
| MspR9I | CCNGG | 4 cut(s) | 444, 539, 637, 1820 |
| MunI | CAATTG | 1 cut(s) | 1813 |
| MvaI | CCWGG | 3 cut(s) | 444, 539, 1820 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 143, 208, 250, 848, 1247, 1588 |
| NciI | CCSGG | 1 cut(s) | 637 |
| NcoI | CCATGG | 2 cut(s) | 162, 318 |
| NdeI | CATATG | 1 cut(s) | 326 |
| NdeII | GATC | 7 cut(s) | 513, 608, 775, 820, 1182, 1498, 1599 |
| NlaIV | GGNNCC | 5 cut(s) | 10, 243, 264, 528, 822 |
| NmuCI | GTSAC | 1 cut(s) | 686 |
| NsiI | ATGCAT | 2 cut(s) | 594, 1593 |
| NspI | RCATGY | 4 cut(s) | 596, 712, 1591, 1595 |
| NspV | TTCGAA | 1 cut(s) | 729 |
| PaeI | GCATGC | 1 cut(s) | 1595 |
| PagI | TCATGA | 1 cut(s) | 1881 |
| PciSI | GCTCTTC | 3 cut(s) | 1097, 1351, 1440 |
| PdmI | GAANNNNTTC | 2 cut(s) | 666, 735 |
| PfeI | GAWTC | 4 cut(s) | 364, 715, 1332, 1741 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1268 |
| PkrI | GCNGC | 8 cut(s) | 36, 97, 169, 201, 213, 850, 1144, 1240 |
| PleI | GAGTC | 4 cut(s) | 430, 868, 1333, 1845 |
| PpsI | GAGTC | 4 cut(s) | 430, 868, 1333, 1845 |
| PpuMI | RGGWCCY | 1 cut(s) | 9 |
| PsiI | TTATAA | 2 cut(s) | 987, 1119 |
| Psp5II | RGGWCCY | 1 cut(s) | 9 |
| Psp6I | CCWGG | 3 cut(s) | 442, 537, 1818 |
| PspFI | CCCAGC | 1 cut(s) | 284 |
| PspGI | CCWGG | 3 cut(s) | 442, 537, 1818 |
| PspN4I | GGNNCC | 5 cut(s) | 10, 243, 264, 528, 822 |
| PspPI | GGNCC | 2 cut(s) | 9, 440 |
| PspPPI | RGGWCCY | 1 cut(s) | 9 |
| PstI | CTGCAG | 2 cut(s) | 853, 1465 |
| PstNI | CAGNNNCTG | 2 cut(s) | 443, 1469 |
| PsuI | RGATCY | 2 cut(s) | 820, 1599 |
| PvuII | CAGCTG | 2 cut(s) | 170, 214 |
| RsaI | GTAC | 3 cut(s) | 884, 1277, 1922 |
| RsaNI | GTAC | 3 cut(s) | 883, 1276, 1921 |
| RseI | CAYNNNNRTG | 2 cut(s) | 684, 1677 |
| SapI | GCTCTTC | 3 cut(s) | 1097, 1351, 1440 |
| SaqAI | TTAA | 6 cut(s) | 339, 453, 1080, 1161, 1221, 1706 |
| SatI | GCNGC | 8 cut(s) | 35, 96, 168, 200, 212, 849, 1143, 1239 |
| Sau3AI | GATC | 7 cut(s) | 513, 608, 775, 820, 1182, 1498, 1599 |
| Sau96I | GGNCC | 2 cut(s) | 9, 440 |
| SchI | GAGTC | 4 cut(s) | 431, 868, 1333, 1845 |
| ScrFI | CCNGG | 4 cut(s) | 444, 539, 637, 1820 |
| SduI | GDGCHC | 3 cut(s) | 529, 844, 1057 |
| SfaNI | GCATC | 3 cut(s) | 489, 579, 630 |
| SfcI | CTRYAG | 3 cut(s) | 849, 1461, 1792 |
| SfuI | TTCGAA | 1 cut(s) | 729 |
| SinI | GGWCC | 1 cut(s) | 9 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 684, 1677 |
| SmlI | CTYRAG | 3 cut(s) | 644, 1070, 1451 |
| SmoI | CTYRAG | 3 cut(s) | 644, 1070, 1451 |
| SphI | GCATGC | 1 cut(s) | 1595 |
| SsiI | CCGC | 8 cut(s) | 32, 35, 93, 96, 199, 1004, 1676, 1797 |
| SspI | AATATT | 2 cut(s) | 457, 577 |
| SspMI | CTAG | 1 cut(s) | 632 |
| StyD4I | CCNGG | 4 cut(s) | 442, 537, 635, 1818 |
| StyI | CCWWGG | 4 cut(s) | 162, 318, 582, 1027 |
| TaaI | ACNGT | 4 cut(s) | 433, 702, 887, 1723 |
| TaiI | ACGT | 1 cut(s) | 747 |
| TaqI | TCGA | 6 cut(s) | 227, 516, 729, 1156, 1360, 1413 |
| TatI | WGTACW | 1 cut(s) | 1920 |
| TauI | GCSGC | 3 cut(s) | 37, 98, 202 |
| TfiI | GAWTC | 4 cut(s) | 364, 715, 1332, 1741 |
| Tru1I | TTAA | 6 cut(s) | 339, 453, 1080, 1161, 1221, 1706 |
| Tru9I | TTAA | 6 cut(s) | 339, 453, 1080, 1161, 1221, 1706 |
| TscAI | CASTG | 5 cut(s) | 139, 243, 858, 1138, 1573 |
| TseFI | GTSAC | 1 cut(s) | 686 |
| TseI | GCWGC | 5 cut(s) | 167, 211, 848, 1142, 1238 |
| Tsp45I | GTSAC | 1 cut(s) | 686 |
| TspDTI | ATGAA | 4 cut(s) | 774, 957, 1395, 1886 |
| TspRI | CASTG | 5 cut(s) | 139, 243, 858, 1138, 1573 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1268 |
| VpaK11BI | GGWCC | 1 cut(s) | 9 |
| XapI | RAATTY | 2 cut(s) | 290, 785 |
| XceI | RCATGY | 4 cut(s) | 596, 712, 1591, 1595 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1298 |
| XmiI | GTMKAC | 2 cut(s) | 558, 1849 |
| XmnI | GAANNNNTTC | 2 cut(s) | 666, 735 |
| XspI | CTAG | 1 cut(s) | 632 |
| Zsp2I | ATGCAT | 2 cut(s) | 594, 1593 |