Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb1

Orthogroup ID

OG0001528

Physical Location & Seq

Genomic Coordinates Forward (+)

3383943 .. 3391699

Gene Structure 7757 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_1g06400.t5

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2007 bp

ATGTTAGGGTCCTCCATAAACCTCATTCTCCCGCCGCCGATTTCATCTTCGCCGTTTCTCTCTCTCACTCGTCTCTCTCTCCCGTCTCATCCCGCCGCCGAATTGTTGAAGGCGTCATCTAGGAAGCTCATTTTTGCAACCAAGGCACGCTCGGTGTCAACCATGGCAGCTGGAGGAGAGAGTGTGGCGAGTGTGAAGCGGCAGTTAGGAAAACTGTTTGAAGTTTCGATTAGAACAGCTGTGCCTGATGTGAATGTCGAGCCAGCAGTTGTTCCGTCGATTGAAAAGTCTGGGAAGAAGTTTGGAGATTATCAGTGTAACAATGCCATGGACATCTGGTCTAAGATTAAGGGAAAGGGAACTGCATTCAACAATCCTAGATCTGTTGGAGAGGCCATCATGGCAAATCTTCCTAAATCCGATATAATAGAGTCATGCACGGTGGCAGGGCCTGGGTTTGTTAATATCGTGTTGGCAAAGGATTGGATGGCTAAGAGCTTACAAAAGATGCTTGTTGATGGTATTGAAACATGGGCACCACAGCTCCAGATCAAACGGGCTGTTGTTGACTTTTCATCACCTAATATTGCCAAGGAAATGCATGTTGGCCATTTGCGATCTACAATTATCGGGGACACACTAGCCAGGATGCTTGAGTTTTCCAAAGTTGAAGTTCTTCGACGTAATCATGTTGGTGACTGGGGAACACAGTTTGGCATGTTGATTCAATACCTTTTCGAAAAGTTTCCAAACGTGGATGATGTTAATGAAATGGAAATCGGTGATCTTCAGGAATTCTATAAGCAGTCAAAAAAGAGATTTGATGAGGATCCGGCCTTCAAGGAGAGAGCACAAGCTGCAGTTGTATCCCTTCAGGGTGGGACTCCCAAGTATCGTGAGGCATGGCTTCAAATTTGTGAAGTCAGCCGTAGAGAGTTTCAAATGGTCTATGAACGCCTTGGAACTCAGTTAGAGGAAAAGGGAGAAAGCTTTTACAACCCATATATTCCTGGGGTTTTAGAGAAGTTGACTGAACAAGGACTGATTGTGGAAAGCGAGGGTGCTCGTGTAATCCATCTTGAGGGTTTTAACATCCCCCTTATTGTTGTGAAGAGCAATGGCAGTTATAATTACGCTTCCACCGATTTGGCAGCTCTTTGGTATCGACTTAATGAGGAGAAAGCTGAATGGATCATATATGTTACTGATGTTGGCCAGCAGCAACACTTTAGTATGTTTTTCAAGAAAGCTGAATGGATCATATATGTTACTGATGTTGGCCAGCAGCAACACTTTAGTATGTTTTTCAAGGCGGCTACACTTGCAGGTTGGCTTCCAGCTGATGGCTCGTACCCTAAAGTTAGCCATGTTGGATTTGGTCTTGTTCTTGGAGAAGATGGCAAACGATTCCGGACTCGCTCTTCTGATGTGGTTCGATTGGTTGATTTACTTGATGAAGCCAAGAGCCGAAGCAAAACAAGTCTTGTCGAGCGTGGTAAGAGTGAAGACTTTACTGAAGAGGAGCTTGAGAAAATTGCAGTGGCTGTTGGTTATGGCGCTGTGAAGTATGCTGATCTGAAGAACAACAGATTGACCAATTACACTTTTGATTTCGATCAAATGCTAAATGATAAGGGAAACACTGCTGTTTACTTGCTATACATGCATGCTCGGATCTGTTCAATAATAAGGAAATCTGGTAAAGACATTGACGAATTGAAAAAAACGGGAGAAATTGTGCTGGGTCATGCAGGTGAGGGTGAATTGGCGCTTCACTTGTTAAAGTTTTCTGAGACCGTGGAGGAGGCTTGCACTAATCTGTTGCCAAGTGTTCTATGCGAATACCTCTATAATCTATCAGAAATCTTTAGCGGGAAGTTCTATTCCAATTGCCAGGTTATCGGGACGCCAGAGGAAACGAGTAGACTGTTACTTTGTGAAGCAACAGGCGTTGTCATGAGGAAATGTTTCCATCTTCTAGGAATCGAAGCAGTGTACAAGATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004812 GO:0004814 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006420 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0010467 GO:0016070 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0022414 GO:0032501 GO:0032502 GO:0034641 GO:0034645 GO:0034660 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0071704 GO:0090304 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

Arg_tRNA_synt_N DALR_1 tRNA-synt_1d

Protein Analysis

669

Amino Acids

74.71

Weight (kDa)

6.25

Isoelectric Point (pI)

42.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Arg_tRNA_synt_N	PF03485	15 - 49	1.9e-07	Arginyl tRNA synthetase N terminal domain
tRNA-synt_1d	PF00750	59 - 407	3.8e-119	tRNA synthetases class I (R), catalytic domain
DALR_1	PF05746	421 - 537	1.2e-29	DALR anticodon binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0001528)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G66530 AT4G26300 AT4G26300 AT4G26300 AT4G26300 AT4G26300
fragaria_vesca	FvH4_1g06400 FvH4_1g06400 FvH4_1g06400 FvH4_1g06400 FvH4_1g06400 FvH4_1g06400
malus_domestica	MD02G1067300.v1.1 MD15G1198200.v1.1 MD15G1199100.v1.1
prunus_persica	Prupe.7G218100_v2.0.a1 Prupe.7G218100_v2.0.a1 Prupe.7G218100_v2.0.a1 Prupe.7G218100_v2.0.a1
pyrus_communis	pycom02g05330 pycom15g17660
rosa_chinensis	RchiOBHm_Chr2g0092141 RchiOBHm_Chr3g0454631 RchiOBHm_Chr3g0454641 RchiOBHm_Chr7g0215671
rosa_laevigata	RLG00000016278 RLG00000025428
rosa_multiflora	Rmu_co8501171.1_g000001 Rmu_sc0000898.1_g000026 Rmu_sc0001792.1_g000032 Rmu_sc0001792.1_g000033 Rmu_sc0013552.1_g000004
rosa_roxburghii	Rroxscaffold_2G00149440 Rroxscaffold_3G00243930
rosa_rugosa	Rorug02G0023000 Rorug02G0023100 Rorug02G0023200 Rorug03G0000700 Rorug03G0000800 Rorug07G0156100
rosa_samantha	Rh2AG068800 Rh2BG068100 Rh2CG069300 Rh2DG067400 Rh3DG062300 Rh7AG292900 Rh7BG285300 Rh7CG313000 Rh7DG296400
rosa_wichuraiana	Rw2G006350 Rw7G024930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1128
AarI	CACCTGC	1 cut(s)	1742
Acc36I	ACCTGC	2 cut(s)	1316, 1742
AccB1I	GGYRCC	1 cut(s)	535
AccB7I	CCANNNNNTGG	1 cut(s)	1343
AccI	GTMKAC	1 cut(s)	1924
AccIII	TCCGGA	1 cut(s)	1410
AciI	CCGC	7 cut(s)	32, 35, 93, 96, 199, 1313, 1872
AclWI	GGATC	5 cut(s)	824, 837, 1199, 1265, 1682
AcoI	YGGCCR	3 cut(s)	607, 1213, 1279
AcsI	RAATTY	2 cut(s)	794, 912
AcuI	CTGAAG	4 cut(s)	773, 857, 1536, 1598
AcyI	GRCGYC	2 cut(s)	113, 1907
AfaI	GTAC	2 cut(s)	1352, 1997
AfiI	CCNNNNNNNGG	2 cut(s)	1081, 1343
AhdI	GACNNNNNGTC	1 cut(s)	337
AjnI	CCWGG	4 cut(s)	451, 644, 1009, 1893
AjuI	GAANNNNNNNTTGG	2 cut(s)	588, 620
Alw21I	GWGCWC	2 cut(s)	853, 1066
Alw26I	GTCTC	3 cut(s)	77, 90, 1787
AlwI	GGATC	5 cut(s)	824, 837, 1199, 1265, 1682
AlwNI	CAGNNNCTG	2 cut(s)	452, 1544
Aor13HI	TCCGGA	1 cut(s)	1410
AoxI	GGCC	6 cut(s)	393, 449, 607, 834, 1213, 1279
ApeKI	GCWGC	5 cut(s)	167, 857, 1151, 1219, 1285
ApoI	RAATTY	2 cut(s)	794, 912
ArsI	GACNNNNNNTTYG	2 cut(s)	1917, 1949
Asp700I	GAANNNNTTC	3 cut(s)	675, 744, 1967
AspLEI	GCGC	2 cut(s)	1559, 1771
AspS9I	GGNCC	2 cut(s)	9, 449
AsuHPI	GGTGA	5 cut(s)	570, 707, 794, 1766, 1772
AsuII	TTCGAA	1 cut(s)	738
AvaII	GGWCC	1 cut(s)	9
BaeGI	GKGCMC	1 cut(s)	538
BalI	TGGCCA	3 cut(s)	609, 1215, 1281
BamHI	GGATCC	1 cut(s)	829
BanI	GGYRCC	1 cut(s)	535
BauI	CACGAG	1 cut(s)	1065
BbsI	GAAGAC	1 cut(s)	1512
Bbv12I	GWGCWC	2 cut(s)	853, 1066
BbvI	GCAGC	5 cut(s)	179, 844, 1163, 1231, 1297
BccI	CCATC	7 cut(s)	404, 481, 512, 1083, 1337, 1391, 1980
BceAI	ACGGC	2 cut(s)	37, 912
BciT130I	CCWGG	4 cut(s)	453, 646, 1011, 1895
BciVI	GTATCC	1 cut(s)	877
BcoDI	GTCTC	3 cut(s)	77, 90, 1787
BfaI	CTAG	4 cut(s)	120, 378, 641, 1979
BfmI	CTRYAG	1 cut(s)	858
BfoI	RGCGCY	2 cut(s)	1560, 1772
BfuAI	ACCTGC	2 cut(s)	1316, 1742
BfuI	GTATCC	1 cut(s)	877
BglI	GCCNNNNNGGC	1 cut(s)	401
BglII	AGATCT	1 cut(s)	380
BisI	GCNGC	9 cut(s)	35, 96, 168, 200, 858, 1152, 1220, 1286, 1314
BlsI	GCNGC	9 cut(s)	36, 97, 169, 201, 859, 1153, 1221, 1287, 1315
Bme1390I	CCNGG	4 cut(s)	453, 646, 1011, 1895
Bme18I	GGWCC	1 cut(s)	9
BmeRI	GACNNNNNGTC	1 cut(s)	337
BmgT120I	GGNCC	2 cut(s)	9, 449
BmiI	GGNNCC	3 cut(s)	10, 537, 831
BmrFI	CCNGG	4 cut(s)	453, 646, 1011, 1895
BmrI	ACTGGG	1 cut(s)	709
BmsI	GCATC	2 cut(s)	498, 639
BmuI	ACTGGG	1 cut(s)	709
BpiI	GAAGAC	1 cut(s)	1512
BpmI	CTGGAG	2 cut(s)	192, 530
Bpu14I	TTCGAA	1 cut(s)	738
BpuEI	CTTGAG	3 cut(s)	674, 1100, 1547
BsaBI	GATNNNNATC	2 cut(s)	828, 1614
BsaHI	GRCGYC	2 cut(s)	113, 1907
BsaI	GGTCTC	1 cut(s)	1787
BsaJI	CCNNGG	8 cut(s)	141, 162, 327, 452, 591, 958, 1010, 1797
BsaWI	WCCGGW	1 cut(s)	1410
BsaXI	ACNNNNNCTCC	6 cut(s)	168, 198, 528, 558, 1722, 1752
Bsc4I	CCNNNNNNNGG	2 cut(s)	1081, 1343
Bse1I	ACTGG	1 cut(s)	704
Bse3DI	GCAATG	1 cut(s)	1123
Bse8I	GATNNNNATC	2 cut(s)	828, 1614
BseAI	TCCGGA	1 cut(s)	1410
BseBI	CCWGG	4 cut(s)	453, 646, 1011, 1895
BseDI	CCNNGG	8 cut(s)	141, 162, 327, 452, 591, 958, 1010, 1797
BseGI	GGATG	5 cut(s)	88, 492, 654, 763, 1092
BseJI	GATNNNNATC	2 cut(s)	828, 1614
BseLI	CCNNNNNNNGG	2 cut(s)	1081, 1343
BseMI	GCAATG	1 cut(s)	1123
BseMII	CTCAG	2 cut(s)	980, 1782
BseNI	ACTGG	1 cut(s)	704
BseRI	GAGGAG	4 cut(s)	189, 1190, 1535, 1817
BseSI	GKGCMC	1 cut(s)	538
BseXI	GCAGC	5 cut(s)	179, 844, 1163, 1231, 1297
BseYI	CCCAGC	1 cut(s)	1741
BshFI	GGCC	6 cut(s)	395, 451, 609, 836, 1215, 1281
BshNI	GGYRCC	1 cut(s)	535
BsiHKAI	GWGCWC	2 cut(s)	853, 1066
BsiSI	CCGG	2 cut(s)	833, 1411
BslFI	GGGAC	3 cut(s)	647, 895, 1918
BslI	CCNNNNNNNGG	2 cut(s)	1081, 1343
BsmAI	GTCTC	3 cut(s)	77, 90, 1787
BsmBI	CGTCTC	2 cut(s)	77, 90
BsmFI	GGGAC	3 cut(s)	647, 895, 1918
BsmI	GAATGC	1 cut(s)	365
BsnI	GGCC	6 cut(s)	395, 451, 609, 836, 1215, 1281
Bso31I	GGTCTC	1 cut(s)	1787
Bsp119I	TTCGAA	1 cut(s)	738
Bsp1286I	GDGCHC	3 cut(s)	538, 853, 1066
Bsp13I	TCCGGA	1 cut(s)	1410
Bsp1407I	TGTACA	1 cut(s)	1995
Bsp19I	CCATGG	2 cut(s)	162, 327
BspACI	CCGC	7 cut(s)	32, 35, 93, 96, 199, 1313, 1872
BspANI	GGCC	6 cut(s)	395, 451, 609, 836, 1215, 1281
BspCNI	CTCAG	2 cut(s)	979, 1783
BspEI	TCCGGA	1 cut(s)	1410
BspHI	TCATGA	1 cut(s)	1956
BspLI	GGNNCC	3 cut(s)	10, 537, 831
BspMAI	CTGCAG	1 cut(s)	862
BspMI	ACCTGC	2 cut(s)	1316, 1742
BspPI	GGATC	5 cut(s)	824, 837, 1199, 1265, 1682
BspQI	GCTCTTC	2 cut(s)	1106, 1426
BspT104I	TTCGAA	1 cut(s)	738
BspT107I	GGYRCC	1 cut(s)	535
BspTNI	GGTCTC	1 cut(s)	1787
BsrDI	GCAATG	1 cut(s)	1123
BsrGI	TGTACA	1 cut(s)	1995
BsrI	ACTGG	1 cut(s)	704
BssECI	CCNNGG	8 cut(s)	141, 162, 327, 452, 591, 958, 1010, 1797
BssNI	GRCGYC	2 cut(s)	113, 1907
BssSI	CACGAG	1 cut(s)	1065
BssT1I	CCWWGG	5 cut(s)	141, 162, 327, 591, 958
Bst2BI	CACGAG	1 cut(s)	1065
Bst2UI	CCWGG	4 cut(s)	453, 646, 1011, 1895
Bst4CI	ACNGT	5 cut(s)	216, 442, 711, 1798, 1929
Bst6I	CTCTTC	3 cut(s)	1106, 1426, 1512
BstACI	GRCGYC	2 cut(s)	113, 1907
BstAPI	GCANNNNNTGC	1 cut(s)	857
BstAUI	TGTACA	1 cut(s)	1995
BstBI	TTCGAA	1 cut(s)	738
BstC8I	GCNNGC	6 cut(s)	148, 264, 1217, 1283, 1668, 1810
BstDEI	CTNAG	4 cut(s)	342, 492, 966, 1791
BstDSI	CCRYGG	3 cut(s)	162, 327, 1797
BstF5I	GGATG	5 cut(s)	88, 492, 654, 763, 1092
BstH2I	RGCGCY	2 cut(s)	1560, 1772
BstHHI	GCGC	2 cut(s)	1559, 1771
BstMAI	GTCTC	3 cut(s)	77, 90, 1787
BstMWI	GCNNNNNNNGC	5 cut(s)	143, 401, 857, 1322, 1663
BstNI	CCWGG	4 cut(s)	453, 646, 1011, 1895
BstNSI	RCATGY	4 cut(s)	605, 721, 1666, 1670
BstSCI	CCNGG	4 cut(s)	451, 644, 1009, 1893
BstSFI	CTRYAG	1 cut(s)	858
BstSLI	GKGCMC	1 cut(s)	538
BstV1I	GCAGC	5 cut(s)	179, 844, 1163, 1231, 1297
BstV2I	GAAGAC	1 cut(s)	1512
BstX2I	RGATCY	3 cut(s)	380, 829, 1674
BstXI	CCANNNNNNTGG	1 cut(s)	1147
BstYI	RGATCY	3 cut(s)	380, 829, 1674
BsuI	GTATCC	1 cut(s)	877
BsuRI	GGCC	6 cut(s)	395, 451, 609, 836, 1215, 1281
BtgI	CCRYGG	3 cut(s)	162, 327, 1797
BtsCI	GGATG	5 cut(s)	88, 492, 654, 763, 1092
BtsI	GCAGTG	3 cut(s)	1545, 1641, 1998
BtsIMutI	CAGTG	4 cut(s)	320, 1545, 1641, 1998
BveI	ACCTGC	2 cut(s)	1316, 1742
Cac8I	GCNNGC	6 cut(s)	148, 264, 1217, 1283, 1668, 1810
CaiI	CAGNNNCTG	2 cut(s)	452, 1544
CciI	TCATGA	1 cut(s)	1956
CfoI	GCGC	2 cut(s)	1559, 1771
Cfr13I	GGNCC	2 cut(s)	9, 449
CseI	GACGC	2 cut(s)	102, 1915
Csp6I	GTAC	2 cut(s)	1351, 1996
CviQI	GTAC	2 cut(s)	1351, 1996
DdeI	CTNAG	4 cut(s)	342, 492, 966, 1791
DriI	GACNNNNNGTC	1 cut(s)	337
EaeI	YGGCCR	3 cut(s)	607, 1213, 1279
Eam1104I	CTCTTC	3 cut(s)	1106, 1426, 1512
Eam1105I	GACNNNNNGTC	1 cut(s)	337
EarI	CTCTTC	3 cut(s)	1106, 1426, 1512
Eco130I	CCWWGG	5 cut(s)	141, 162, 327, 591, 958
Eco31I	GGTCTC	1 cut(s)	1787
Eco47I	GGWCC	1 cut(s)	9
Eco57I	CTGAAG	4 cut(s)	773, 857, 1536, 1598
EcoO109I	RGGNCCY	2 cut(s)	9, 449
EcoRI	GAATTC	1 cut(s)	794
EcoRII	CCWGG	4 cut(s)	451, 644, 1009, 1893
EcoT14I	CCWWGG	5 cut(s)	141, 162, 327, 591, 958
EcoT22I	ATGCAT	2 cut(s)	603, 1668
ErhI	CCWWGG	5 cut(s)	141, 162, 327, 591, 958
Esp3I	CGTCTC	2 cut(s)	77, 90
FalI	AAGNNNNNCTT	2 cut(s)	1509, 1541
FaqI	GGGAC	3 cut(s)	647, 895, 1918
FauI	CCCGC	3 cut(s)	39, 100, 1865
FblI	GTMKAC	1 cut(s)	1924
Fnu4HI	GCNGC	9 cut(s)	35, 96, 168, 200, 858, 1152, 1220, 1286, 1314
FokI	GGATG	5 cut(s)	75, 499, 661, 770, 1079
Fsp4HI	GCNGC	9 cut(s)	35, 96, 168, 200, 858, 1152, 1220, 1286, 1314
FspBI	CTAG	4 cut(s)	120, 378, 641, 1979
GlaI	GCGC	2 cut(s)	1558, 1770
GluI	GCNGC	9 cut(s)	35, 96, 168, 200, 858, 1152, 1220, 1286, 1314
GsaI	CCCAGC	1 cut(s)	1745
GsuI	CTGGAG	2 cut(s)	192, 530
HaeII	RGCGCY	2 cut(s)	1560, 1772
HaeIII	GGCC	6 cut(s)	395, 451, 609, 836, 1215, 1281
HapII	CCGG	2 cut(s)	833, 1411
HgaI	GACGC	2 cut(s)	102, 1915
HhaI	GCGC	2 cut(s)	1559, 1771
Hin1I	GRCGYC	2 cut(s)	113, 1907
Hin6I	GCGC	2 cut(s)	1557, 1769
HinP1I	GCGC	2 cut(s)	1557, 1769
HincII	GTYRAC	3 cut(s)	159, 568, 1029
HindII	GTYRAC	3 cut(s)	159, 568, 1029
HindIII	AAGCTT	1 cut(s)	988
HinfI	GANTC	6 cut(s)	431, 724, 883, 1407, 1414, 1983
HpaII	CCGG	2 cut(s)	833, 1411
HphI	GGTGA	5 cut(s)	570, 707, 794, 1766, 1772
Hpy166II	GTNNAC	6 cut(s)	159, 568, 1029, 1651, 1925, 1996
Hpy188I	TCNGA	6 cut(s)	421, 1426, 1578, 1674, 1792, 1861
Hpy188III	TCNNGA	8 cut(s)	547, 791, 896, 1079, 1243, 1411, 1903, 1957
Hpy8I	GTNNAC	6 cut(s)	159, 568, 1029, 1651, 1925, 1996
Hpy99I	CGWCG	2 cut(s)	280, 684
HpyAV	CCTTC	3 cut(s)	103, 847, 881
HpyCH4III	ACNGT	5 cut(s)	216, 442, 711, 1798, 1929
HpyCH4IV	ACGT	2 cut(s)	682, 753
HpyF10VI	GCNNNNNNNGC	5 cut(s)	143, 401, 857, 1322, 1663
HpyF3I	CTNAG	4 cut(s)	342, 492, 966, 1791
HpySE526I	ACGT	2 cut(s)	682, 753
Hsp92I	GRCGYC	2 cut(s)	113, 1907
HspAI	GCGC	2 cut(s)	1557, 1769
Kpn2I	TCCGGA	1 cut(s)	1410
LguI	GCTCTTC	2 cut(s)	1106, 1426
LmnI	GCTCC	2 cut(s)	549, 1522
Lsp1109I	GCAGC	5 cut(s)	179, 844, 1163, 1231, 1297
LweI	GCATC	2 cut(s)	498, 639
MaeI	CTAG	4 cut(s)	120, 378, 641, 1979
MaeII	ACGT	2 cut(s)	682, 753
MaeIII	GTNAC	5 cut(s)	317, 695, 1201, 1267, 1929
MfeI	CAATTG	1 cut(s)	1888
MflI	RGATCY	3 cut(s)	380, 829, 1674
MhlI	GDGCHC	3 cut(s)	538, 853, 1066
MlsI	TGGCCA	3 cut(s)	609, 1215, 1281
MluNI	TGGCCA	3 cut(s)	609, 1215, 1281
MlyI	GAGTC	3 cut(s)	440, 877, 1408
MmeI	TCCRAC	2 cut(s)	367, 1351
Mox20I	TGGCCA	3 cut(s)	609, 1215, 1281
Mph1103I	ATGCAT	2 cut(s)	603, 1668
MroI	TCCGGA	1 cut(s)	1410
MroXI	GAANNNNTTC	3 cut(s)	675, 744, 1967
MscI	TGGCCA	3 cut(s)	609, 1215, 1281
MseI	TTAA	6 cut(s)	348, 462, 765, 1089, 1170, 1781
MslI	CAYNNNNRTG	2 cut(s)	693, 1752
Msp20I	TGGCCA	3 cut(s)	609, 1215, 1281
MspA1I	CMGCKG	3 cut(s)	170, 239, 1340
MspI	CCGG	2 cut(s)	833, 1411
MspR9I	CCNGG	4 cut(s)	453, 646, 1011, 1895
MunI	CAATTG	1 cut(s)	1888
Mva1269I	GAATGC	1 cut(s)	365
MvaI	CCWGG	4 cut(s)	453, 646, 1011, 1895
MwoI	GCNNNNNNNGC	5 cut(s)	143, 401, 857, 1322, 1663
NcoI	CCATGG	2 cut(s)	162, 327
NlaIV	GGNNCC	3 cut(s)	10, 537, 831
NmuCI	GTSAC	1 cut(s)	695
NsiI	ATGCAT	2 cut(s)	603, 1668
NspI	RCATGY	4 cut(s)	605, 721, 1666, 1670
NspV	TTCGAA	1 cut(s)	738
PaeI	GCATGC	1 cut(s)	1670
PagI	TCATGA	1 cut(s)	1956
PaqCI	CACCTGC	1 cut(s)	1742
PciSI	GCTCTTC	2 cut(s)	1106, 1426
PcsI	WCGNNNNNNNCGW	1 cut(s)	1140
PctI	GAATGC	1 cut(s)	365
PdmI	GAANNNNTTC	3 cut(s)	675, 744, 1967
PfeI	GAWTC	3 cut(s)	724, 1407, 1983
PflMI	CCANNNNNTGG	1 cut(s)	1343
PkrI	GCNGC	9 cut(s)	36, 97, 169, 201, 859, 1153, 1221, 1287, 1315
PleI	GAGTC	3 cut(s)	439, 877, 1408
PpsI	GAGTC	3 cut(s)	439, 877, 1408
PpuMI	RGGWCCY	1 cut(s)	9
PsiI	TTATAA	1 cut(s)	1128
Psp5II	RGGWCCY	1 cut(s)	9
Psp6I	CCWGG	4 cut(s)	451, 644, 1009, 1893
PspFI	CCCAGC	1 cut(s)	1741
PspGI	CCWGG	4 cut(s)	451, 644, 1009, 1893
PspN4I	GGNNCC	3 cut(s)	10, 537, 831
PspPI	GGNCC	2 cut(s)	9, 449
PspPPI	RGGWCCY	1 cut(s)	9
PstI	CTGCAG	1 cut(s)	862
PstNI	CAGNNNCTG	2 cut(s)	452, 1544
PsuI	RGATCY	3 cut(s)	380, 829, 1674
PvuII	CAGCTG	3 cut(s)	170, 239, 1340
RsaI	GTAC	2 cut(s)	1352, 1997
RsaNI	GTAC	2 cut(s)	1351, 1996
RseI	CAYNNNNRTG	2 cut(s)	693, 1752
SapI	GCTCTTC	2 cut(s)	1106, 1426
SaqAI	TTAA	6 cut(s)	348, 462, 765, 1089, 1170, 1781
SatI	GCNGC	9 cut(s)	35, 96, 168, 200, 858, 1152, 1220, 1286, 1314
Sau96I	GGNCC	2 cut(s)	9, 449
SchI	GAGTC	3 cut(s)	440, 877, 1408
ScrFI	CCNGG	4 cut(s)	453, 646, 1011, 1895
SduI	GDGCHC	3 cut(s)	538, 853, 1066
SfaNI	GCATC	2 cut(s)	498, 639
SfcI	CTRYAG	1 cut(s)	858
SfuI	TTCGAA	1 cut(s)	738
SinI	GGWCC	1 cut(s)	9
SmiMI	CAYNNNNRTG	2 cut(s)	693, 1752
SmlI	CTYRAG	3 cut(s)	653, 1079, 1526
SmoI	CTYRAG	3 cut(s)	653, 1079, 1526
SphI	GCATGC	1 cut(s)	1670
SsiI	CCGC	7 cut(s)	32, 35, 93, 96, 199, 1313, 1872
SspI	AATATT	1 cut(s)	586
SspMI	CTAG	4 cut(s)	120, 378, 641, 1979
StyD4I	CCNGG	4 cut(s)	451, 644, 1009, 1893
StyI	CCWWGG	5 cut(s)	141, 162, 327, 591, 958
TaaI	ACNGT	5 cut(s)	216, 442, 711, 1798, 1929
TaiI	ACGT	2 cut(s)	685, 756
TatI	WGTACW	1 cut(s)	1995
TauI	GCSGC	4 cut(s)	37, 98, 202, 1316
TfiI	GAWTC	3 cut(s)	724, 1407, 1983
Tru1I	TTAA	6 cut(s)	348, 462, 765, 1089, 1170, 1781
Tru9I	TTAA	6 cut(s)	348, 462, 765, 1089, 1170, 1781
TscAI	CASTG	4 cut(s)	320, 1545, 1648, 1998
TseFI	GTSAC	1 cut(s)	695
TseI	GCWGC	5 cut(s)	167, 857, 1151, 1219, 1285
Tsp45I	GTSAC	1 cut(s)	695
TspDTI	ATGAA	5 cut(s)	33, 564, 783, 966, 1470
TspGWI	ACGGA	1 cut(s)	264
TspRI	CASTG	4 cut(s)	320, 1545, 1648, 1998
Van91I	CCANNNNNTGG	1 cut(s)	1343
VpaK11BI	GGWCC	1 cut(s)	9
XapI	RAATTY	2 cut(s)	794, 912
XceI	RCATGY	4 cut(s)	605, 721, 1666, 1670
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1373
XmiI	GTMKAC	1 cut(s)	1924
XmnI	GAANNNNTTC	3 cut(s)	675, 744, 1967
XspI	CTAG	4 cut(s)	120, 378, 641, 1979
Zsp2I	ATGCAT	2 cut(s)	603, 1668

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.