AT1G66670

clp protease proteolytic subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0003024
Physical Location & Seq
Reverse (-)
24863734 .. 24865848
2115 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G66670.1

Sequence Viewer

Length: 930 bp
ATGGAGATGAGTTTGCGTCTCGCTTCATCTTCAACCTCAAACCCAATTTGTCTACTAAACCCTGGAAAAAACCTTAATTTCCCAATCCGAAACCATAGAATCCCTAAAACTTCGAAACCCTTTTGCGTTAGGTCTTCAATGAGCTTGTCTAAACCACCCAGACAAACCTTATCTAGTAACTGGGATGTATCTAGCTTCTCCATTGATTCCGTTGCTCAATCTCCTTCAAGACTCCCAAGTTTCGAAGAACTCGATACCACCAACATGTTGCTCCGTCAAAGAATCGTCTTTTTGGGTTCTCAGGTTGATGATATGACGGCGGATTTGGTTATAAGTCAGCTATTGTTACTAGATGCTGAGGACTCAGAAAGAGACATTACGCTTTTTATCAATTCACCCGGTGGATCTATTACTGCTGGGATGGGAATATATGATGCAATGAAACAATGTAAGGCGGATGTATCTACTGTTTGCTTAGGGTTAGCTGCATCTATGGGTGCGTTTCTTCTTGCTTCTGGTTCAAAAGGGAAACGGTATTGTATGCCTAACTCTAAAGTTATGATCCATCAGCCACTTGGTACTGCTGGAGGCAAAGCAACGGAAATGAGCATACGTATAAGAGAAATGATGTACCACAAGATTAAACTTAACAAAATCTTCTCTAGAATCACTGGGAAGCCTGAATCAGAGATCGAAAGTGACACAGACCGTGATAACTTCTTGAATCCATGGGAGGCGAAAGAATATGGTTTGATCGACGCTGTAATCGATGATGGGAAACCGGGACTAATCGCTCCAATTGGAGATGGTACTCCTCCTCCTAAAACCAAAGTCTGGGATCTTTGGAAAGTCGAAGGAACCAAGAAAGACAACACTAACTTGCCATCTGAGCGCTCCATGACACAGAATGGTTATGCCGCCATTGAATAG

Protein Analysis

309

Amino Acids

33.92

Weight (kDa)

7.59

Isoelectric Point (pI)

45.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLP_protease PF00574 84 - 257 4e-73 Clp protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 332
AccB7I CCANNNNNTGG 1 cut(s) 834
AccI GTMKAC 1 cut(s) 52
AciI CCGC 3 cut(s) 320, 455, 918
AclWI GGATC 3 cut(s) 412, 556, 846
AdeI CACNNNGTG 1 cut(s) 401
AfaI GTAC 3 cut(s) 580, 632, 811
AfeI AGCGCT 1 cut(s) 893
AfiI CCNNNNNNNGG 1 cut(s) 834
AflIII ACRYGT 1 cut(s) 264
AgsI TTSAA 6 cut(s) 33, 138, 228, 522, 724, 926
AjnI CCWGG 1 cut(s) 61
AluBI AGCT 4 cut(s) 144, 195, 340, 485
AluI AGCT 4 cut(s) 144, 195, 340, 485
Alw26I GTCTC 2 cut(s) 23, 366
AlwI GGATC 3 cut(s) 412, 556, 846
Aor51HI AGCGCT 1 cut(s) 893
ApeKI GCWGC 1 cut(s) 485
ArsI GACNNNNNNTTYG 2 cut(s) 307, 339
AspLEI GCGC 1 cut(s) 894
AsuC2I CCSGG 2 cut(s) 399, 783
AsuHPI GGTGA 1 cut(s) 387
AsuII TTCGAA 2 cut(s) 113, 243
BbsI GAAGAC 1 cut(s) 126
BbvCI CCTCAGC 1 cut(s) 357
BbvI GCAGC 1 cut(s) 472
BccI CCATC 5 cut(s) 415, 573, 767, 800, 892
BceAI ACGGC 1 cut(s) 333
BciT130I CCWGG 1 cut(s) 63
BcnI CCSGG 2 cut(s) 399, 783
BcoDI GTCTC 2 cut(s) 23, 366
BfaI CTAG 4 cut(s) 174, 192, 350, 663
BfoI RGCGCY 1 cut(s) 895
BisI GCNGC 2 cut(s) 486, 918
BlsI GCNGC 2 cut(s) 487, 919
Bme1390I CCNGG 3 cut(s) 63, 399, 783
BmiI GGNNCC 1 cut(s) 859
BmrFI CCNGG 3 cut(s) 63, 399, 783
BmrI ACTGGG 2 cut(s) 190, 681
BmsI GCATC 3 cut(s) 343, 424, 497
BmuI ACTGGG 2 cut(s) 190, 681
BpiI GAAGAC 1 cut(s) 126
BpmI CTGGAG 1 cut(s) 606
Bpu10I CCTNAGC 2 cut(s) 357, 475
Bpu14I TTCGAA 2 cut(s) 113, 243
BpuMI CCSGG 2 cut(s) 399, 783
Bsa29I ATCGAT 1 cut(s) 768
BsaAI YACGTR 1 cut(s) 614
BsaJI CCNNGG 2 cut(s) 61, 728
BsaXI ACNNNNNCTCC 2 cut(s) 802, 832
Bsc4I CCNNNNNNNGG 1 cut(s) 834
Bse1I ACTGG 2 cut(s) 185, 676
Bse3DI GCAATG 1 cut(s) 444
BseBI CCWGG 1 cut(s) 63
BseCI ATCGAT 1 cut(s) 768
BseDI CCNNGG 2 cut(s) 61, 728
BseGI GGATG 3 cut(s) 190, 426, 463
BseLI CCNNNNNNNGG 1 cut(s) 834
BseMI GCAATG 1 cut(s) 444
BseMII CTCAG 4 cut(s) 314, 348, 378, 879
BseNI ACTGG 2 cut(s) 185, 676
BseRI GAGGAG 2 cut(s) 804, 807
BseXI GCAGC 1 cut(s) 472
BseYI CCCAGC 1 cut(s) 416
BshVI ATCGAT 1 cut(s) 768
BsiSI CCGG 2 cut(s) 399, 782
BslFI GGGAC 1 cut(s) 798
BslI CCNNNNNNNGG 1 cut(s) 834
BsmAI GTCTC 2 cut(s) 23, 366
BsmBI CGTCTC 1 cut(s) 23
BsmFI GGGAC 1 cut(s) 798
Bsp119I TTCGAA 2 cut(s) 113, 243
Bsp143I GATC 5 cut(s) 404, 561, 690, 753, 838
Bsp19I CCATGG 1 cut(s) 728
BspACI CCGC 3 cut(s) 320, 455, 918
BspCNI CTCAG 4 cut(s) 313, 349, 377, 880
BspDI ATCGAT 1 cut(s) 768
BspLI GGNNCC 1 cut(s) 859
BspPI GGATC 3 cut(s) 412, 556, 846
BspT104I TTCGAA 2 cut(s) 113, 243
BsrDI GCAATG 1 cut(s) 444
BsrI ACTGG 2 cut(s) 185, 676
BssECI CCNNGG 2 cut(s) 61, 728
BssMI GATC 5 cut(s) 404, 561, 690, 753, 838
BssT1I CCWWGG 1 cut(s) 728
Bst2UI CCWGG 1 cut(s) 63
Bst4CI ACNGT 3 cut(s) 469, 534, 710
BstBAI YACGTR 1 cut(s) 614
BstBI TTCGAA 2 cut(s) 113, 243
BstDEI CTNAG 5 cut(s) 300, 357, 364, 475, 888
BstDSI CCRYGG 1 cut(s) 728
BstF5I GGATG 3 cut(s) 190, 426, 463
BstH2I RGCGCY 1 cut(s) 895
BstHHI GCGC 1 cut(s) 894
BstKTI GATC 5 cut(s) 407, 564, 693, 756, 841
BstMAI GTCTC 2 cut(s) 23, 366
BstMBI GATC 5 cut(s) 404, 561, 690, 753, 838
BstMWI GCNNNNNNNGC 1 cut(s) 889
BstNI CCWGG 1 cut(s) 63
BstNSI RCATGY 1 cut(s) 268
BstSCI CCNGG 3 cut(s) 61, 397, 781
BstSNI TACGTA 1 cut(s) 614
BstV1I GCAGC 1 cut(s) 472
BstV2I GAAGAC 1 cut(s) 126
BstX2I RGATCY 2 cut(s) 404, 838
BstYI RGATCY 2 cut(s) 404, 838
Bsu15I ATCGAT 1 cut(s) 768
BsuTUI ATCGAT 1 cut(s) 768
BtgI CCRYGG 1 cut(s) 728
BtsCI GGATG 3 cut(s) 190, 426, 463
BtsIMutI CAGTG 1 cut(s) 669
CfoI GCGC 1 cut(s) 894
ClaI ATCGAT 1 cut(s) 768
CseI GACGC 2 cut(s) 5, 767
Csp6I GTAC 3 cut(s) 579, 631, 810
CviAII CATG 3 cut(s) 265, 729, 898
CviJI RGCY 6 cut(s) 144, 195, 340, 485, 571, 679
CviKI_1 RGCY 6 cut(s) 144, 195, 340, 485, 571, 679
CviQI GTAC 3 cut(s) 579, 631, 810
DdeI CTNAG 5 cut(s) 300, 357, 364, 475, 888
DpnI GATC 5 cut(s) 406, 563, 692, 755, 840
DpnII GATC 5 cut(s) 404, 561, 690, 753, 838
DraIII CACNNNGTG 1 cut(s) 401
EciI GGCGGA 2 cut(s) 335, 470
Eco105I TACGTA 1 cut(s) 614
Eco130I CCWWGG 1 cut(s) 728
Eco47III AGCGCT 1 cut(s) 893
EcoRII CCWGG 1 cut(s) 61
EcoT14I CCWWGG 1 cut(s) 728
ErhI CCWWGG 1 cut(s) 728
Esp3I CGTCTC 1 cut(s) 23
FaeI CATG 3 cut(s) 268, 732, 901
FaqI GGGAC 1 cut(s) 798
FatI CATG 3 cut(s) 264, 728, 897
FblI GTMKAC 1 cut(s) 52
Fnu4HI GCNGC 2 cut(s) 486, 918
FokI GGATG 3 cut(s) 197, 433, 470
Fsp4HI GCNGC 2 cut(s) 486, 918
FspBI CTAG 4 cut(s) 174, 192, 350, 663
GlaI GCGC 1 cut(s) 893
GluI GCNGC 2 cut(s) 486, 918
GsaI CCCAGC 1 cut(s) 420
GsuI CTGGAG 1 cut(s) 606
HaeII RGCGCY 1 cut(s) 895
HapII CCGG 2 cut(s) 399, 782
HgaI GACGC 2 cut(s) 5, 767
HhaI GCGC 1 cut(s) 894
Hin1II CATG 3 cut(s) 268, 732, 901
Hin6I GCGC 1 cut(s) 892
HinP1I GCGC 1 cut(s) 892
HinfI GANTC 8 cut(s) 99, 206, 231, 282, 362, 666, 683, 724
HpaII CCGG 2 cut(s) 399, 782
HphI GGTGA 1 cut(s) 387
Hpy166II GTNNAC 1 cut(s) 53
Hpy188I TCNGA 4 cut(s) 89, 367, 688, 889
Hpy188III TCNNGA 3 cut(s) 228, 663, 721
Hpy8I GTNNAC 1 cut(s) 53
Hpy99I CGWCG 1 cut(s) 761
HpyAV CCTTC 2 cut(s) 234, 848
HpyCH4III ACNGT 3 cut(s) 469, 534, 710
HpyCH4IV ACGT 1 cut(s) 613
HpyCH4V TGCA 2 cut(s) 437, 488
HpyF10VI GCNNNNNNNGC 1 cut(s) 889
HpyF3I CTNAG 5 cut(s) 300, 357, 364, 475, 888
HpySE526I ACGT 1 cut(s) 613
Hsp92II CATG 3 cut(s) 268, 732, 901
HspAI GCGC 1 cut(s) 892
Kzo9I GATC 5 cut(s) 404, 561, 690, 753, 838
LmnI GCTCC 3 cut(s) 276, 799, 899
Lsp1109I GCAGC 1 cut(s) 472
LweI GCATC 3 cut(s) 343, 424, 497
MaeI CTAG 4 cut(s) 174, 192, 350, 663
MaeII ACGT 1 cut(s) 613
MaeIII GTNAC 3 cut(s) 176, 345, 698
MalI GATC 5 cut(s) 406, 563, 692, 755, 840
MboI GATC 5 cut(s) 404, 561, 690, 753, 838
MboII GAAGA 5 cut(s) 21, 126, 257, 497, 649
MfeI CAATTG 1 cut(s) 798
MflI RGATCY 2 cut(s) 404, 838
MluCI AATT 4 cut(s) 45, 76, 391, 798
MlyI GAGTC 2 cut(s) 225, 356
MnlI CCTC 6 cut(s) 46, 352, 581, 727, 825, 828
MseI TTAA 3 cut(s) 75, 642, 648
MslI CAYNNNNRTG 1 cut(s) 263
MspI CCGG 2 cut(s) 399, 782
MspR9I CCNGG 3 cut(s) 63, 399, 783
MunI CAATTG 1 cut(s) 798
MvaI CCWGG 1 cut(s) 63
MwoI GCNNNNNNNGC 1 cut(s) 889
NciI CCSGG 2 cut(s) 399, 783
NcoI CCATGG 1 cut(s) 728
NdeII GATC 5 cut(s) 404, 561, 690, 753, 838
NlaIII CATG 3 cut(s) 268, 732, 901
NlaIV GGNNCC 1 cut(s) 859
NmuCI GTSAC 1 cut(s) 698
NspI RCATGY 1 cut(s) 268
NspV TTCGAA 2 cut(s) 113, 243
PciI ACATGT 1 cut(s) 264
PcsI WCGNNNNNNNCGW 2 cut(s) 249, 765
PfeI GAWTC 6 cut(s) 99, 206, 282, 666, 683, 724
PflMI CCANNNNNTGG 1 cut(s) 834
PkrI GCNGC 2 cut(s) 487, 919
PleI GAGTC 2 cut(s) 225, 356
PpsI GAGTC 2 cut(s) 225, 356
Ppu21I YACGTR 1 cut(s) 614
PscI ACATGT 1 cut(s) 264
PsiI TTATAA 1 cut(s) 332
Psp6I CCWGG 1 cut(s) 61
PspFI CCCAGC 1 cut(s) 416
PspGI CCWGG 1 cut(s) 61
PspN4I GGNNCC 1 cut(s) 859
PsuI RGATCY 2 cut(s) 404, 838
RsaI GTAC 3 cut(s) 580, 632, 811
RsaNI GTAC 3 cut(s) 579, 631, 810
RseI CAYNNNNRTG 1 cut(s) 263
SaqAI TTAA 3 cut(s) 75, 642, 648
SatI GCNGC 2 cut(s) 486, 918
Sau3AI GATC 5 cut(s) 404, 561, 690, 753, 838
SchI GAGTC 2 cut(s) 225, 356
ScrFI CCNGG 3 cut(s) 63, 399, 783
SfaNI GCATC 3 cut(s) 343, 424, 497
SfuI TTCGAA 2 cut(s) 113, 243
SmiMI CAYNNNNRTG 1 cut(s) 263
SnaBI TACGTA 1 cut(s) 614
Sse9I AATT 4 cut(s) 45, 76, 391, 798
SsiI CCGC 3 cut(s) 320, 455, 918
SspMI CTAG 4 cut(s) 174, 192, 350, 663
StyD4I CCNGG 3 cut(s) 61, 397, 781
StyI CCWWGG 1 cut(s) 728
TaaI ACNGT 3 cut(s) 469, 534, 710
TaiI ACGT 1 cut(s) 616
TaqI TCGA 7 cut(s) 113, 243, 252, 693, 756, 768, 852
TasI AATT 4 cut(s) 45, 76, 391, 798
TauI GCSGC 1 cut(s) 920
TfiI GAWTC 6 cut(s) 99, 206, 282, 666, 683, 724
Tru1I TTAA 3 cut(s) 75, 642, 648
Tru9I TTAA 3 cut(s) 75, 642, 648
TscAI CASTG 1 cut(s) 676
TseFI GTSAC 1 cut(s) 698
TseI GCWGC 1 cut(s) 485
Tsp45I GTSAC 1 cut(s) 698
TspDTI ATGAA 2 cut(s) 15, 455
TspGWI ACGGA 3 cut(s) 199, 263, 614
TspRI CASTG 1 cut(s) 676
Van91I CCANNNNNTGG 1 cut(s) 834
XbaI TCTAGA 1 cut(s) 662
XceI RCATGY 1 cut(s) 268
XcmI CCANNNNNNNNNTGG 1 cut(s) 572
XmiI GTMKAC 1 cut(s) 52
XspI CTAG 4 cut(s) 174, 192, 350, 663
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.