Rh1CG086900

ATP-dependent Clp protease proteolytic subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0003024
Physical Location & Seq
Reverse (-)
18194762 .. 18202590
7829 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG086900.1

Sequence Viewer

Length: 957 bp
ATGATGACATACGCAGCCTCAGCTTCAAGGCCTCTCTGCTTCAACACCCAACCGGCCATTGTGAACAGCAATGGTCTTAATAGCACTAGCGCTTCTAGTATCTTCGATTGCAGCAGAACAACAAGGAGGAAGCGCGTCGGCCTAGTTCAAGCCTCGTCGCCTACCAGACCCACCTTGTCCAGCAATTGGGATTTTCAGTTCGCAGCTACTACTACTACTACTTCCTCTCCACGCGTCCCCAGATTCGAAGAGCTCGACACCACCAACATGCTCCTCCGTCAGCGAATCATCTTCTTGGGTTCTCAGGTGGATGATATGACGGCGGATTTGATAATAAGCCAACTGCTATTTCTGGATGCTGAAGACCCCAAAAAGGATATTACATTGTTTATCAATTCGCCTGGTGGCTCGGTTACTGCTGGAATGGGAATATATGATGCAATGAAATTGTGCAAGGCAGATGTTTCAACTGTTTGCTTGGGGCTTGCTGCTTCTATGGGTGCATTTCTCCTTGCTTCCGGTTCAAAAGGAAAGAGGTACTGCATGCCCAATGCAAGAGTGATGATCCATCAACCTCTTGGAACTGCTGGAGGCAAAGCAACAGAAATGGGTATACGAATTAGAGAAATGGCGTACCACAAGATTAAATTGAACAAAATATTGTCAAGAATCACAGGGAAGCCTTTAGACCAGGTTGAGGAGGACACAGACCGTGATAATTTCATGAATCCCTGGGAAGCTAGGGATTATGGGTTGATTGATGAAGTTATTGATGATGGAAAGCCAGGGTTAGTTGCACCAATTGGAGATTCCACACCTCCACCCAAAACAAAAGTCTGGGATATGTGGAAAGTTGAAGGTACCAAGAAATCCAGAAGGAATTTACCCTCGGAGCAGAAAATTTCAGATAAGGGACGTGAGAATGAGAAAGGTTTGGAAGCACCTACTGCAACATAG

Protein Analysis

318

Amino Acids

34.83

Weight (kDa)

8.88

Isoelectric Point (pI)

35.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLP_protease PF00574 85 - 258 5.5e-75 Clp protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 860
AccB1I GGYRCC 1 cut(s) 860
AccB7I CCANNNNNTGG 1 cut(s) 186
AccI GTMKAC 1 cut(s) 613
AccII CGCG 2 cut(s) 135, 234
AciI CCGC 1 cut(s) 323
AclWI GGATC 1 cut(s) 559
AcoI YGGCCR 1 cut(s) 54
AcsI RAATTY 2 cut(s) 880, 900
AcuI CTGAAG 1 cut(s) 381
AfaI GTAC 3 cut(s) 539, 635, 862
AfeI AGCGCT 1 cut(s) 91
AfiI CCNNNNNNNGG 3 cut(s) 186, 373, 697
AflIII ACRYGT 1 cut(s) 232
AgsI TTSAA 7 cut(s) 27, 43, 149, 468, 525, 652, 857
AjiI CACGTC 1 cut(s) 917
AjnI CCWGG 4 cut(s) 400, 690, 731, 784
AjuI GAANNNNNNNTTGG 4 cut(s) 333, 365, 793, 825
AloI GAACNNNNNNTCC 2 cut(s) 182, 214
AluBI AGCT 4 cut(s) 23, 206, 253, 740
AluI AGCT 4 cut(s) 23, 206, 253, 740
Alw21I GWGCWC 1 cut(s) 255
AlwI GGATC 1 cut(s) 559
Aor51HI AGCGCT 1 cut(s) 91
AoxI GGCC 3 cut(s) 29, 54, 139
ApeKI GCWGC 4 cut(s) 14, 111, 203, 488
ApoI RAATTY 2 cut(s) 880, 900
ArsI GACNNNNNNTTYG 4 cut(s) 310, 342, 819, 851
Asp718I GGTACC 1 cut(s) 860
AspLEI GCGC 2 cut(s) 92, 135
AsuII TTCGAA 1 cut(s) 246
BanI GGYRCC 1 cut(s) 860
BanII GRGCYC 1 cut(s) 255
BbsI GAAGAC 1 cut(s) 369
Bbv12I GWGCWC 1 cut(s) 255
BbvCI CCTCAGC 1 cut(s) 19
BbvI GCAGC 4 cut(s) 26, 123, 215, 475
BccI CCATC 2 cut(s) 576, 770
BceAI ACGGC 1 cut(s) 336
BciT130I CCWGG 4 cut(s) 402, 692, 733, 786
BfaI CTAG 4 cut(s) 87, 96, 143, 741
BfoI RGCGCY 1 cut(s) 93
BisI GCNGC 4 cut(s) 15, 112, 204, 489
BlsI GCNGC 4 cut(s) 16, 113, 205, 490
Bme1390I CCNGG 4 cut(s) 402, 692, 733, 786
BmgBI CACGTC 1 cut(s) 917
BmiI GGNNCC 1 cut(s) 862
BmrFI CCNGG 4 cut(s) 402, 692, 733, 786
BmsI GCATC 2 cut(s) 346, 427
BpiI GAAGAC 1 cut(s) 369
BpmI CTGGAG 1 cut(s) 609
Bpu10I CCTNAGC 1 cut(s) 19
Bpu14I TTCGAA 1 cut(s) 246
BsaJI CCNNGG 4 cut(s) 731, 732, 785, 888
BsaWI WCCGGW 1 cut(s) 518
BsaXI ACNNNNNCTCC 2 cut(s) 211, 241
Bsc4I CCNNNNNNNGG 3 cut(s) 186, 373, 697
Bse118I RCCGGY 1 cut(s) 52
Bse3DI GCAATG 2 cut(s) 76, 447
BseBI CCWGG 4 cut(s) 402, 692, 733, 786
BseDI CCNNGG 4 cut(s) 731, 732, 785, 888
BseGI GGATG 2 cut(s) 316, 361
BseLI CCNNNNNNNGG 3 cut(s) 186, 373, 697
BseMI GCAATG 2 cut(s) 76, 447
BseMII CTCAG 2 cut(s) 33, 317
BseRI GAGGAG 2 cut(s) 263, 713
BseXI GCAGC 4 cut(s) 26, 123, 215, 475
Bsh1236I CGCG 2 cut(s) 135, 234
BshFI GGCC 3 cut(s) 31, 56, 141
BshNI GGYRCC 1 cut(s) 860
BsiHKAI GWGCWC 1 cut(s) 255
BsiSI CCGG 2 cut(s) 53, 519
BslFI GGGAC 2 cut(s) 221, 927
BslI CCNNNNNNNGG 3 cut(s) 186, 373, 697
BsmFI GGGAC 2 cut(s) 221, 927
BsnI GGCC 3 cut(s) 31, 56, 141
Bsp119I TTCGAA 1 cut(s) 246
Bsp1286I GDGCHC 1 cut(s) 255
Bsp143I GATC 1 cut(s) 564
BspACI CCGC 1 cut(s) 323
BspANI GGCC 3 cut(s) 31, 56, 141
BspCNI CTCAG 2 cut(s) 32, 316
BspFNI CGCG 2 cut(s) 135, 234
BspHI TCATGA 1 cut(s) 723
BspLI GGNNCC 1 cut(s) 862
BspPI GGATC 1 cut(s) 559
BspQI GCTCTTC 1 cut(s) 243
BspT104I TTCGAA 1 cut(s) 246
BspT107I GGYRCC 1 cut(s) 860
BsrDI GCAATG 2 cut(s) 76, 447
BsrFI RCCGGY 1 cut(s) 52
BssAI RCCGGY 1 cut(s) 52
BssECI CCNNGG 4 cut(s) 731, 732, 785, 888
BssMI GATC 1 cut(s) 564
BssNAI GTATAC 1 cut(s) 614
Bst1107I GTATAC 1 cut(s) 614
Bst2UI CCWGG 4 cut(s) 402, 692, 733, 786
Bst4CI ACNGT 2 cut(s) 472, 713
Bst6I CTCTTC 1 cut(s) 243
BstAPI GCANNNNNTGC 1 cut(s) 947
BstBI TTCGAA 1 cut(s) 246
BstC8I GCNNGC 2 cut(s) 486, 545
BstDEI CTNAG 2 cut(s) 19, 303
BstF5I GGATG 2 cut(s) 316, 361
BstFNI CGCG 2 cut(s) 135, 234
BstH2I RGCGCY 1 cut(s) 93
BstHHI GCGC 2 cut(s) 92, 135
BstKTI GATC 1 cut(s) 567
BstMBI GATC 1 cut(s) 564
BstMWI GCNNNNNNNGC 2 cut(s) 20, 947
BstNI CCWGG 4 cut(s) 402, 692, 733, 786
BstNSI RCATGY 2 cut(s) 271, 547
BstSCI CCNGG 4 cut(s) 400, 690, 731, 784
BstUI CGCG 2 cut(s) 135, 234
BstV1I GCAGC 4 cut(s) 26, 123, 215, 475
BstV2I GAAGAC 1 cut(s) 369
BstZ17I GTATAC 1 cut(s) 614
BsuRI GGCC 3 cut(s) 31, 56, 141
BtrI CACGTC 1 cut(s) 917
BtsCI GGATG 2 cut(s) 316, 361
Cac8I GCNNGC 2 cut(s) 486, 545
CciI TCATGA 1 cut(s) 723
CfoI GCGC 2 cut(s) 92, 135
Cfr10I RCCGGY 1 cut(s) 52
CseI GACGC 2 cut(s) 124, 223
CsiI ACCWGGT 1 cut(s) 690
Csp6I GTAC 3 cut(s) 538, 634, 861
CviAII CATG 3 cut(s) 268, 544, 724
CviQI GTAC 3 cut(s) 538, 634, 861
DdeI CTNAG 2 cut(s) 19, 303
DpnI GATC 1 cut(s) 566
DpnII GATC 1 cut(s) 564
EaeI YGGCCR 1 cut(s) 54
Eam1104I CTCTTC 1 cut(s) 243
EarI CTCTTC 1 cut(s) 243
EciI GGCGGA 1 cut(s) 338
Ecl136II GAGCTC 1 cut(s) 253
Eco147I AGGCCT 1 cut(s) 31
Eco24I GRGCYC 1 cut(s) 255
Eco47III AGCGCT 1 cut(s) 91
Eco53kI GAGCTC 1 cut(s) 253
Eco57I CTGAAG 1 cut(s) 381
EcoICRI GAGCTC 1 cut(s) 253
EcoRII CCWGG 4 cut(s) 400, 690, 731, 784
EcoT38I GRGCYC 1 cut(s) 255
FaeI CATG 3 cut(s) 271, 547, 727
FaqI GGGAC 2 cut(s) 221, 927
FatI CATG 3 cut(s) 267, 543, 723
FblI GTMKAC 1 cut(s) 613
Fnu4HI GCNGC 4 cut(s) 15, 112, 204, 489
FokI GGATG 2 cut(s) 323, 368
FriOI GRGCYC 1 cut(s) 255
Fsp4HI GCNGC 4 cut(s) 15, 112, 204, 489
FspBI CTAG 4 cut(s) 87, 96, 143, 741
GlaI GCGC 2 cut(s) 91, 134
GluI GCNGC 4 cut(s) 15, 112, 204, 489
GsuI CTGGAG 1 cut(s) 609
HaeII RGCGCY 1 cut(s) 93
HaeIII GGCC 3 cut(s) 31, 56, 141
HapII CCGG 2 cut(s) 53, 519
HgaI GACGC 2 cut(s) 124, 223
HhaI GCGC 2 cut(s) 92, 135
Hin1II CATG 3 cut(s) 271, 547, 727
Hin6I GCGC 2 cut(s) 90, 133
HinP1I GCGC 2 cut(s) 90, 133
HinfI GANTC 5 cut(s) 243, 285, 669, 727, 809
HpaII CCGG 2 cut(s) 53, 519
Hpy166II GTNNAC 2 cut(s) 64, 614
Hpy188I TCNGA 2 cut(s) 892, 907
Hpy188III TCNNGA 4 cut(s) 353, 666, 724, 873
Hpy8I GTNNAC 2 cut(s) 64, 614
Hpy99I CGWCG 2 cut(s) 140, 160
HpyAV CCTTC 2 cut(s) 851, 870
HpyCH4III ACNGT 2 cut(s) 472, 713
HpyCH4IV ACGT 1 cut(s) 916
HpyCH4V TGCA 8 cut(s) 111, 440, 453, 503, 543, 554, 797, 950
HpyF10VI GCNNNNNNNGC 2 cut(s) 20, 947
HpyF3I CTNAG 2 cut(s) 19, 303
HpySE526I ACGT 1 cut(s) 916
Hsp92II CATG 3 cut(s) 271, 547, 727
HspAI GCGC 2 cut(s) 90, 133
KpnI GGTACC 1 cut(s) 864
Kzo9I GATC 1 cut(s) 564
LguI GCTCTTC 1 cut(s) 243
LmnI GCTCC 2 cut(s) 276, 892
Lsp1109I GCAGC 4 cut(s) 26, 123, 215, 475
LweI GCATC 2 cut(s) 346, 427
MabI ACCWGGT 1 cut(s) 690
MaeI CTAG 4 cut(s) 87, 96, 143, 741
MaeII ACGT 1 cut(s) 916
MaeIII GTNAC 1 cut(s) 412
MalI GATC 1 cut(s) 566
MboI GATC 1 cut(s) 564
MboII GAAGA 4 cut(s) 94, 260, 283, 374
MfeI CAATTG 2 cut(s) 184, 801
MhlI GDGCHC 1 cut(s) 255
MluCI AATT 9 cut(s) 184, 394, 446, 618, 647, 718, 801, 880, 900
MluI ACGCGT 1 cut(s) 232
MseI TTAA 2 cut(s) 78, 645
MslI CAYNNNNRTG 1 cut(s) 266
MspI CCGG 2 cut(s) 53, 519
MspR9I CCNGG 4 cut(s) 402, 692, 733, 786
MunI CAATTG 2 cut(s) 184, 801
MvaI CCWGG 4 cut(s) 402, 692, 733, 786
MvnI CGCG 2 cut(s) 135, 234
MwoI GCNNNNNNNGC 2 cut(s) 20, 947
NdeII GATC 1 cut(s) 564
NlaIII CATG 3 cut(s) 271, 547, 727
NlaIV GGNNCC 1 cut(s) 862
NspI RCATGY 2 cut(s) 271, 547
NspV TTCGAA 1 cut(s) 246
PaeI GCATGC 1 cut(s) 547
PagI TCATGA 1 cut(s) 723
PasI CCCWGGG 1 cut(s) 732
PceI AGGCCT 1 cut(s) 31
PciSI GCTCTTC 1 cut(s) 243
PcsI WCGNNNNNNNCGW 1 cut(s) 252
PfeI GAWTC 5 cut(s) 243, 285, 669, 727, 809
PflMI CCANNNNNTGG 1 cut(s) 186
PkrI GCNGC 4 cut(s) 16, 113, 205, 490
Psp124BI GAGCTC 1 cut(s) 255
Psp6I CCWGG 4 cut(s) 400, 690, 731, 784
PspGI CCWGG 4 cut(s) 400, 690, 731, 784
PspN4I GGNNCC 1 cut(s) 862
RsaI GTAC 3 cut(s) 539, 635, 862
RsaNI GTAC 3 cut(s) 538, 634, 861
RseI CAYNNNNRTG 1 cut(s) 266
SacI GAGCTC 1 cut(s) 255
SapI GCTCTTC 1 cut(s) 243
SaqAI TTAA 2 cut(s) 78, 645
SatI GCNGC 4 cut(s) 15, 112, 204, 489
Sau3AI GATC 1 cut(s) 564
ScrFI CCNGG 4 cut(s) 402, 692, 733, 786
SduI GDGCHC 1 cut(s) 255
SexAI ACCWGGT 1 cut(s) 690
SfaNI GCATC 2 cut(s) 346, 427
SfuI TTCGAA 1 cut(s) 246
SmiMI CAYNNNNRTG 1 cut(s) 266
SphI GCATGC 1 cut(s) 547
Sse9I AATT 9 cut(s) 184, 394, 446, 618, 647, 718, 801, 880, 900
SseBI AGGCCT 1 cut(s) 31
SsiI CCGC 1 cut(s) 323
SspI AATATT 1 cut(s) 660
SspMI CTAG 4 cut(s) 87, 96, 143, 741
SstI GAGCTC 1 cut(s) 255
StuI AGGCCT 1 cut(s) 31
StyD4I CCNGG 4 cut(s) 400, 690, 731, 784
TaaI ACNGT 2 cut(s) 472, 713
TaiI ACGT 1 cut(s) 919
TaqI TCGA 3 cut(s) 105, 246, 255
TasI AATT 9 cut(s) 184, 394, 446, 618, 647, 718, 801, 880, 900
TfiI GAWTC 5 cut(s) 243, 285, 669, 727, 809
Tru1I TTAA 2 cut(s) 78, 645
Tru9I TTAA 2 cut(s) 78, 645
TseI GCWGC 4 cut(s) 14, 111, 203, 488
TspDTI ATGAA 4 cut(s) 458, 712, 740, 777
TspGWI ACGGA 1 cut(s) 266
Van91I CCANNNNNTGG 1 cut(s) 186
XapI RAATTY 2 cut(s) 880, 900
XceI RCATGY 2 cut(s) 271, 547
XcmI CCANNNNNNNNNTGG 1 cut(s) 575
XmiI GTMKAC 1 cut(s) 613
XspI CTAG 4 cut(s) 87, 96, 143, 741
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.