Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000001

Orthogroup ID

OG0003024

Physical Location & Seq

Genomic Coordinates Reverse (-)

11917925 .. 11923138

Gene Structure 5214 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug01G0073100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3591 bp

ATGCTTGAGGACGCTCTTAGTCCTACGATCACGAACGCTCTTAGTTCGCGTGTTGGAAAACAGGCTGCGGGGATGTGCGAGGTAGGCGAGTTTTGGGTGAGGCTAGCTGAACGCTGGAGCTACGGGGGTAGGCTACGGGTGCTGCAGGGCCGCGGGGCTGGGCGCGCTGGCAGGCTGGCTGTCGCGGGGGCATGCTATAGGGCTGAGGATTGCGGGGGGCAGACGCTAGCGTGGGCTAGCTGTGTGCGGTGGTTGCGGAGCGCAGGGGCGCGTAGGAGCTGCGGGGGTAGCGAGCGCAGGGGCTGCAGGGCTGGGGCTTGCGGGAGATTTGGTGAGAAGAGTTTTAAGGCCTCCCTTTCCAAATATACTACCTCTCCTCATCCATTTAGCTATCATGTGTTCTTGAGTTTTAGAGGTGAAGACACTCGAAAGAACTTCACTGATCACCTCTACACAGCCCTTGTCAACGCCGGACTTCATACTTTCCGGGATGACCCTGAACTCGAAAGAGGTGAAAATATCAAGGACAAGCTTGATAAAGCCATTCAACAGTCACAAAGTTCTGTCATTGTGTTTTCAAAAGACTATACCTCTTCCAGATGGTGTCTGGACGAGCTTGTGATGATCCTTGAACGCAAAAGGACTTCCGATCATGTCGTTTTACCGGTCTTCTATGACGTTGATCCATCTCAACTGAGGAAGCAGGCAGAAACTCTTGCAAAGATTCCTAAATACCAAGAAAATCAATCGTCGGAGAGGTTGAAAGGATGGCGGGCAGCACTTAGAGAAGTTGCAGATCTAGCAGGAATGGTTTTAGAAAATGAAGCTGACGGGCACGAATCAAAGTTCATCCAAAATATCGTTAGAGTGATCCAAGACAAACTAGGTCGTCGTGTGCCCCTGACCGACGTCCAGGAGGTGTTACAGAAGACGTTGTTGTTTATTAACCCTGTTCTCGATGATGCCGAGGGGAAGCAACTGAACAACCCAACAGTGACGGCATGGCTCAATGAGCTTAAAGACGCAGTCTATGATACTGAGGACCTATTGCAGGAGATCAAGACAGAAGTATTACGGCGAAATATGGAACCTAAATCTGGAACTAGCACGAGTAAGGTACAAGATCTGATCTCTACTCCTCCATCTCATGTTTTTGACTTGGCATTAATATGCCCCAGGGTAGAGGAAGTTCTTAACAGACTAGACTCCATTATGGAGGATGATGAGAAGGAAAAACTTATGAAATTGTTGCTATCAGATGATCAGAGTGGCAATAAAATAAGTGTGATTCCTATTGTGGGTATGGGTGGGATTGGAAAGACCACCCTTCCCCAGTTTCTATACAATGATGATAGAGTCAAACAGCATTTTGACCTCCAAGCATGGGCATGTGTTTCTGAAAAATTTGATGTCCTTCGGATCTCACAACAAATTTATGAGTCCCTTACTTCAGATGCTTTCTTCATTATGAACTTAGATCTGCTTCAGTCAAAATTGAAGGTTGCTTTGATGGGGAAAAGGATTTTCCTTGTTCTTGATGACTTGTGGAATAAAAATTACACTGAATGGGATGTTTTAAGGCGTCCATTTCAATTTGGAGCACATGGAAGTAAGATTATTGTCACAACACGCAATCAAGATGTTGCATGCATGGTGTGTACTCTTGAAGCTCACCGTCTCAGGCCGATGTCTGAGGAAGATGGCTGGTTGTTATTTGAAAAACATGCCTTCAAAAATGCAGGTGTTGGTGCATACTTACATCTTGAAGAAATCGGTAGACAAATTGTTCGAAAGAGCAATGGTCTTCCTTTGGCTATTAAGTCTCTTGGGGGTCTCTTATGTTCTAAATTAACTGTGGGGGAATGGGAAAGACTTGAGTTATTGTCAAAATTACAATATTTAAGAGCGCTCACGTTATCAGGGCACGATATTGAAGAATTGCCTGATTCAATTAACAACTTGAAACAACTTAGGTACTTGGACTTGTCTTCGACACCTATCAAAAAGTTACCTGATAGAATTTATACTCTGTATAACTTGCAAGTGTTGTTGTTGTCACGTTGTTCTGCTCTTACTGAACTGCCAGCCAACTTGGGAAGATTGATCAACTTGAGCCATCTGGATACTTTTGGTACAATAAGGTTAAAGAAGATGCCACCTCTTATGGGCAATTTGAAAGACCTCCAGATTTTAACCGTATTTGTGGTAGACAAGCACACTGCAAGGGATAATTTGCTAGAGTTGAAGAAGCTTCAAAATTTGTGCGGAAGACTTTGTATTTCAGGGCTTGGTGATGTTGTGCATGGCAGCGCTTTGGAGGCCCACATTCTGAGGGACAAGAAGTTTCTTAGTGATCTAGTTTTGAATTGGGGAGGAAGGCATGATAGTGAAGTAGGCAATAATACTGTAGACGAAATAGAAGTGCTTGAGAAGCTCCAACCTCACCCAAACCTAGAAAGGCTTATAGTTACAGATTATGGAGGCAGATTCTTTCCAGGTTGGTCAGGATATTATACTTCCTTGCGACCAAGAAAAGCTCTAGTTTATCTTGAGCTTGTTGATTGTGTAAATTGTGTCTCCTTGCCACCATTGGGACAGCTACCTTGCCTCAGAGAGCTTGATATTTCTGGGTTACATGGAGTGCTGTCCATTGGTTCCGAGTTTTATTATGGTGACAATACTACTCGTGCAAACAAACCATTTAGGTCTCTACAATCGTTGAGATTCTATGATATGAGCGGGTGGCAAGAATGGTGTCATGTTGGAGGTGAGGAGGATGAAGGTGGGGTTTTTCCTAGTCTTGTTCAGCTTGATGTATCAGACTGTCCTAATCTTTCCGGGAGATTAGCGTTAGACAGCTTCCCGATGCTGAAAGAGGTCGACTTGATCAGGACTAACCTATCCCTAGTCACCAGGTCACGGGACTCTAAATTATCCCTGAATCAGTTGCGATTACAGTCGTGCTCAAAAGTTGTAGCTTTTCCCAGTGGAGGGTTACATGCACCCAACTTGACGGAAATAGAAATCCGACGATATGAGAAGTTGAGTTCGTTGCCGGAACAAATGGAGACCCTTCTCCCATCTCTTGTGTCTTTGTCGTTATGGGATTGCCCAGAACTGGAATGTGTTCCGGAAGGGGGATTCCCGTCAAAGTTAAAATCACTTAACATCATGGACAGTAAAAAACTGAATGCAAAGAGTACACAGAATATGAACAAAGCTCTGCGAAGACTGACCTCTCTTGAAGAACTGAGACTTGACTTCGACTGCGACGAAGAATGCGAAAAGGTAGATTGGTTTTCGGAGGGCCTCTTGCCCAGCACTCTCGATTACCTCCTGATCCACCGTCTCAAGTGTGAGGCCATCGACGGCGCCAAATGGTTTGGCCATCTCAACTCTCTTCGAAGCTTGGAGATTAGCAACTGCTCTTCGCTCCGGTGCTTGCCAGATTGTGGGTTACCCTCGTCTCTTACTCATCTATTTATCCATGGATGTCCTTTGCTTGAAAAACGGTGCCAAAGAGAGACAGGTGAAGATTGGTCCAAGATTGCTCACATCTCTATGATATTGATCGATTGGCATACGATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009368 GO:0009507 GO:0009526 GO:0009532 GO:0009534 GO:0009536 GO:0009570 GO:0009579 GO:0009840 GO:0009941 GO:0031967 GO:0031975 GO:0031976 GO:0031984 GO:0032991 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko04112 ko04212 map04112 map04212

Pfam Domains

Protein Families

CLP_protease

Protein Analysis

1196

Amino Acids

134.89

Weight (kDa)

6.44

Isoelectric Point (pI)

50.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TIR	PF01582	131 - 304	6.2e-44	TIR domain
TIR_2	PF13676	133 - 232	4.3e-17	TIR domain
Rx_N	PF18052	282 - 367	5.9e-16	Rx N-terminal domain
NB-ARC	PF00931	408 - 579	2.3e-34	NB-ARC domain
LRR_14	PF23598	626 - 961	1.3e-26	Leucine-rich repeat region
LRR_R13L1-DRL21	PF25019	747 - 877	1.3e-29	R13L1/DRL21 LRRs
LRR_R13L1-DRL21	PF25019	1054 - 1165	7.3e-07	R13L1/DRL21 LRRs

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0003024)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G66670
fragaria_vesca	FvH4_7g04110
malus_domestica	MD02G1275200.v1.1 MD07G1043100.v1.1
prunus_persica	Prupe.2G045800_v2.0.a1 Prupe.2G045800_v2.0.a1 Prupe.2G045800_v2.0.a1
pyrus_communis	pycom02g23530 pycom07g03020
rosa_chinensis	RchiOBHm_Chr1g0327981 RchiOBHm_Chr1g0372191
rosa_laevigata	RLG00000008647 RLG00000012938 RLG00000021509 RLG00000029950
rosa_multiflora	Rmu_sc0000064.1_g000027 Rmu_sc0000272.1_g000043 Rmu_sc0001755.1_g000019 Rmu_sc0003977.1_g000001 Rmu_sc0007782.1_g000018 Rmu_ssc0000309.1_g000031
rosa_roxburghii	Rroxscaffold_4G00322040
rosa_rugosa	Rorug01G0073100
rosa_samantha	Rh1AG089900 Rh1BG071800 Rh1BG342100 Rh1CG086900 Rh1DG093600 Rh2BG432000 Rh2CG303000 Rh6AG065100 Rh6AG270700 Rh6BG088100 Rh6DG224400 Rh7DG308700
rosa_wichuraiana	Rw0G022860 Rw1G006940 Rw1G006960

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1730
AatII	GACGTC	1 cut(s)	912
Acc36I	ACCTGC	1 cut(s)	1730
AccB1I	GGYRCC	2 cut(s)	3371, 3513
AccB7I	CCANNNNNTGG	1 cut(s)	3452
AccBSI	CCGCTC	1 cut(s)	2739
AccI	GTMKAC	4 cut(s)	1777, 2208, 2409, 2880
AccII	CGCG	5 cut(s)	49, 153, 165, 185, 271
AccIII	TCCGGA	1 cut(s)	3130
AclWI	GGATC	5 cut(s)	619, 677, 865, 1427, 3334
AcoI	YGGCCR	1 cut(s)	3385
AcsI	RAATTY	4 cut(s)	1403, 1431, 2019, 2257
AcuI	CTGAAG	2 cut(s)	1434, 1469
AcyI	GRCGYC	3 cut(s)	909, 1582, 3372
AfaI	GTAC	5 cut(s)	1119, 1660, 1976, 2134, 3202
AfeI	AGCGCT	2 cut(s)	1908, 2311
AgeI	ACCGGT	1 cut(s)	664
AjnI	CCWGG	4 cut(s)	912, 1175, 2494, 2912
Alw21I	GWGCWC	2 cut(s)	1603, 2966
AlwI	GGATC	5 cut(s)	619, 677, 865, 1427, 3334
AlwNI	CAGNNNCTG	1 cut(s)	303
Aor13HI	TCCGGA	1 cut(s)	3130
Aor51HI	AGCGCT	2 cut(s)	1908, 2311
AoxI	GGCC	7 cut(s)	148, 348, 1682, 2319, 3307, 3360, 3385
ApeKI	GCWGC	6 cut(s)	65, 142, 279, 303, 776, 2307
ApoI	RAATTY	4 cut(s)	1403, 1431, 2019, 2257
ArsI	GACNNNNNNTTYG	2 cut(s)	1770, 1802
AseI	ATTAAT	1 cut(s)	1166
AsiGI	ACCGGT	1 cut(s)	664
Asp700I	GAANNNNTTC	1 cut(s)	3126
AspLEI	GCGC	8 cut(s)	165, 167, 263, 271, 297, 1909, 2312, 3374
AspS9I	GGNCC	5 cut(s)	148, 1042, 2320, 3307, 3540
AsuC2I	CCSGG	2 cut(s)	488, 2839
AsuII	TTCGAA	2 cut(s)	1789, 3403
AsuNHI	GCTAGC	3 cut(s)	103, 226, 236
AvaII	GGWCC	2 cut(s)	1042, 3540
BaeGI	GKGCMC	3 cut(s)	837, 900, 1926
BalI	TGGCCA	1 cut(s)	3387
BanI	GGYRCC	2 cut(s)	3371, 3513
BauI	CACGAG	2 cut(s)	1108, 2685
BbsI	GAAGAC	7 cut(s)	426, 661, 935, 1796, 1980, 2275, 3235
Bbv12I	GWGCWC	2 cut(s)	1603, 2966
BbvCI	CCTCAGC	1 cut(s)	204
BbvI	GCAGC	6 cut(s)	52, 129, 266, 290, 788, 2319
BceAI	ACGGC	3 cut(s)	1014, 1091, 3385
BciT130I	CCWGG	4 cut(s)	914, 1177, 2496, 2914
BciVI	GTATCC	1 cut(s)	2116
BclI	TGATCA	4 cut(s)	442, 1261, 2103, 2886
BcnI	CCSGG	2 cut(s)	488, 2839
BfmI	CTRYAG	4 cut(s)	143, 196, 304, 2406
BfoI	RGCGCY	3 cut(s)	1910, 2313, 3375
BfuAI	ACCTGC	1 cut(s)	1730
BfuI	GTATCC	1 cut(s)	2116
BglII	AGATCT	3 cut(s)	796, 1123, 1477
BisI	GCNGC	7 cut(s)	66, 143, 151, 280, 304, 777, 2308
BlsI	GCNGC	7 cut(s)	67, 144, 152, 281, 305, 778, 2309
Bme1390I	CCNGG	6 cut(s)	488, 914, 1177, 2496, 2839, 2914
Bme18I	GGWCC	2 cut(s)	1042, 3540
BmgT120I	GGNCC	5 cut(s)	148, 1042, 2320, 3307, 3540
BmiI	GGNNCC	4 cut(s)	1089, 2656, 3373, 3515
BmrFI	CCNGG	6 cut(s)	488, 914, 1177, 2496, 2839, 2914
BmrI	ACTGGG	2 cut(s)	1327, 2979
BmsI	GCATC	4 cut(s)	952, 1444, 2142, 2856
BmtI	GCTAGC	3 cut(s)	107, 230, 240
BmuI	ACTGGG	2 cut(s)	1327, 2979
BoxI	GACNNNNGTC	1 cut(s)	908
BpiI	GAAGAC	7 cut(s)	426, 661, 935, 1796, 1980, 2275, 3235
BpmI	CTGGAG	2 cut(s)	136, 2168
Bpu10I	CCTNAGC	1 cut(s)	204
Bpu14I	TTCGAA	2 cut(s)	1789, 3403
BpuEI	CTTGAG	7 cut(s)	26, 424, 1895, 2131, 2447, 2570, 3335
BpuMI	CCSGG	2 cut(s)	488, 2839
Bsa29I	ATCGAT	1 cut(s)	3573
BsaBI	GATNNNNATC	1 cut(s)	3569
BsaHI	GRCGYC	3 cut(s)	909, 1582, 3372
BsaI	GGTCTC	3 cut(s)	1838, 2712, 3062
BsaJI	CCNNGG	5 cut(s)	151, 966, 1175, 1176, 3487
BsaWI	WCCGGW	3 cut(s)	664, 3130, 3435
BsaXI	ACNNNNNCTCC	4 cut(s)	316, 346, 358, 388
Bse118I	RCCGGY	1 cut(s)	664
Bse1I	ACTGG	3 cut(s)	1333, 2985, 3123
Bse3DI	GCAATG	1 cut(s)	1804
Bse8I	GATNNNNATC	1 cut(s)	3569
BseAI	TCCGGA	1 cut(s)	3130
BseBI	CCWGG	4 cut(s)	914, 1177, 2496, 2914
BseCI	ATCGAT	1 cut(s)	3573
BseDI	CCNNGG	5 cut(s)	151, 966, 1175, 1176, 3487
BseGI	GGATG	9 cut(s)	78, 379, 496, 773, 849, 1225, 1576, 2782, 3497
BseJI	GATNNNNATC	1 cut(s)	3569
BseMI	GCAATG	1 cut(s)	1804
BseMII	CTCAG	8 cut(s)	195, 686, 1029, 1683, 1693, 2321, 2623, 3242
BseNI	ACTGG	3 cut(s)	1333, 2985, 3123
BsePI	GCGCGC	1 cut(s)	163
BseRI	GAGGAG	3 cut(s)	366, 1128, 2786
BseSI	GKGCMC	3 cut(s)	837, 900, 1926
BseXI	GCAGC	6 cut(s)	52, 129, 266, 290, 788, 2319
BseYI	CCCAGC	3 cut(s)	158, 311, 3317
Bsh1236I	CGCG	5 cut(s)	49, 153, 165, 185, 271
BshFI	GGCC	7 cut(s)	150, 350, 1684, 2321, 3309, 3362, 3387
BshNI	GGYRCC	2 cut(s)	3371, 3513
BshTI	ACCGGT	1 cut(s)	664
BshVI	ATCGAT	1 cut(s)	3573
BsiHKAI	GWGCWC	2 cut(s)	1603, 2966
BsiSI	CCGG	7 cut(s)	471, 487, 665, 2838, 3056, 3131, 3436
BslFI	GGGAC	4 cut(s)	1426, 2348, 2607, 2936
BsmBI	CGTCTC	3 cut(s)	1682, 3353, 3471
BsmFI	GGGAC	4 cut(s)	1426, 2348, 2607, 2936
BsmI	GAATGC	2 cut(s)	3196, 3284
BsnI	GGCC	7 cut(s)	150, 350, 1684, 2321, 3309, 3362, 3387
Bso31I	GGTCTC	3 cut(s)	1838, 2712, 3062
Bsp119I	TTCGAA	2 cut(s)	1789, 3403
Bsp1286I	GDGCHC	5 cut(s)	837, 900, 1603, 1926, 2966
Bsp13I	TCCGGA	1 cut(s)	3130
Bsp19I	CCATGG	1 cut(s)	3487
BspANI	GGCC	7 cut(s)	150, 350, 1684, 2321, 3309, 3362, 3387
BspCNI	CTCAG	8 cut(s)	196, 687, 1030, 1684, 1692, 2322, 2622, 3243
BspDI	ATCGAT	1 cut(s)	3573
BspEI	TCCGGA	1 cut(s)	3130
BspFNI	CGCG	5 cut(s)	49, 153, 165, 185, 271
BspLI	GGNNCC	4 cut(s)	1089, 2656, 3373, 3515
BspMAI	CTGCAG	2 cut(s)	147, 308
BspMI	ACCTGC	1 cut(s)	1730
BspOI	GCTAGC	3 cut(s)	107, 230, 240
BspPI	GGATC	5 cut(s)	619, 677, 865, 1427, 3334
BspQI	GCTCTTC	1 cut(s)	3433
BspT104I	TTCGAA	2 cut(s)	1789, 3403
BspT107I	GGYRCC	2 cut(s)	3371, 3513
BspTNI	GGTCTC	3 cut(s)	1838, 2712, 3062
BsrBI	CCGCTC	1 cut(s)	2739
BsrDI	GCAATG	1 cut(s)	1804
BsrFI	RCCGGY	1 cut(s)	664
BsrI	ACTGG	3 cut(s)	1333, 2985, 3123
BssAI	RCCGGY	1 cut(s)	664
BssECI	CCNNGG	5 cut(s)	151, 966, 1175, 1176, 3487
BssHII	GCGCGC	1 cut(s)	163
BssNI	GRCGYC	3 cut(s)	909, 1582, 3372
BssSI	CACGAG	2 cut(s)	1108, 2685
BssT1I	CCWWGG	1 cut(s)	3487
Bst2BI	CACGAG	2 cut(s)	1108, 2685
Bst2UI	CCWGG	4 cut(s)	914, 1177, 2496, 2914
Bst6I	CTCTTC	4 cut(s)	332, 598, 3405, 3433
BstACI	GRCGYC	3 cut(s)	909, 1582, 3372
BstAPI	GCANNNNNTGC	1 cut(s)	303
BstBI	TTCGAA	2 cut(s)	1789, 3403
BstDSI	CCRYGG	2 cut(s)	151, 3487
BstEII	GGTNACC	1 cut(s)	3456
BstENI	CCTNNNNNAGG	1 cut(s)	1049
BstF5I	GGATG	9 cut(s)	78, 379, 496, 773, 849, 1225, 1576, 2782, 3497
BstFNI	CGCG	5 cut(s)	49, 153, 165, 185, 271
BstH2I	RGCGCY	3 cut(s)	1910, 2313, 3375
BstHHI	GCGC	8 cut(s)	165, 167, 263, 271, 297, 1909, 2312, 3374
BstNI	CCWGG	4 cut(s)	914, 1177, 2496, 2914
BstNSI	RCATGY	5 cut(s)	195, 1392, 1650, 1727, 3002
BstPAI	GACNNNNGTC	1 cut(s)	908
BstPI	GGTNACC	1 cut(s)	3456
BstSCI	CCNGG	6 cut(s)	486, 912, 1175, 2494, 2837, 2912
BstSFI	CTRYAG	4 cut(s)	143, 196, 304, 2406
BstSLI	GKGCMC	3 cut(s)	837, 900, 1926
BstUI	CGCG	5 cut(s)	49, 153, 165, 185, 271
BstV1I	GCAGC	6 cut(s)	52, 129, 266, 290, 788, 2319
BstV2I	GAAGAC	7 cut(s)	426, 661, 935, 1796, 1980, 2275, 3235
BstX2I	RGATCY	4 cut(s)	796, 1123, 1419, 1477
BstYI	RGATCY	4 cut(s)	796, 1123, 1419, 1477
Bsu15I	ATCGAT	1 cut(s)	3573
BsuI	GTATCC	1 cut(s)	2116
BsuRI	GGCC	7 cut(s)	150, 350, 1684, 2321, 3309, 3362, 3387
BsuTUI	ATCGAT	1 cut(s)	3573
BtgI	CCRYGG	2 cut(s)	151, 3487
BtsCI	GGATG	9 cut(s)	78, 379, 496, 773, 849, 1225, 1576, 2782, 3497
BtsI	GCAGTG	1 cut(s)	2217
BtsIMutI	CAGTG	5 cut(s)	438, 999, 1560, 2217, 2992
BveI	ACCTGC	1 cut(s)	1730
CaiI	CAGNNNCTG	1 cut(s)	303
CfoI	GCGC	8 cut(s)	165, 167, 263, 271, 297, 1909, 2312, 3374
Cfr10I	RCCGGY	1 cut(s)	664
Cfr13I	GGNCC	5 cut(s)	148, 1042, 2320, 3307, 3540
Cfr42I	CCGCGG	1 cut(s)	154
ClaI	ATCGAT	1 cut(s)	3573
CseI	GACGC	4 cut(s)	20, 232, 1031, 1571
CsiI	ACCWGGT	1 cut(s)	2912
Csp6I	GTAC	5 cut(s)	1118, 1659, 1975, 2133, 3201
CspAI	ACCGGT	1 cut(s)	664
CviQI	GTAC	5 cut(s)	1118, 1659, 1975, 2133, 3201
DinI	GGCGCC	1 cut(s)	3373
EaeI	YGGCCR	1 cut(s)	3385
Eam1104I	CTCTTC	4 cut(s)	332, 598, 3405, 3433
EarI	CTCTTC	4 cut(s)	332, 598, 3405, 3433
Eco130I	CCWWGG	1 cut(s)	3487
Eco147I	AGGCCT	1 cut(s)	350
Eco31I	GGTCTC	3 cut(s)	1838, 2712, 3062
Eco47I	GGWCC	2 cut(s)	1042, 3540
Eco47III	AGCGCT	2 cut(s)	1908, 2311
Eco57I	CTGAAG	2 cut(s)	1434, 1469
Eco91I	GGTNACC	1 cut(s)	3456
EcoNI	CCTNNNNNAGG	1 cut(s)	1049
EcoO109I	RGGNCCY	2 cut(s)	1042, 3307
EcoO65I	GGTNACC	1 cut(s)	3456
EcoRII	CCWGG	4 cut(s)	912, 1175, 2494, 2912
EcoT14I	CCWWGG	1 cut(s)	3487
EcoT22I	ATGCAT	1 cut(s)	1652
EgeI	GGCGCC	1 cut(s)	3373
EheI	GGCGCC	1 cut(s)	3373
ErhI	CCWWGG	1 cut(s)	3487
Esp3I	CGTCTC	3 cut(s)	1682, 3353, 3471
FaqI	GGGAC	4 cut(s)	1426, 2348, 2607, 2936
FauI	CCCGC	8 cut(s)	61, 146, 178, 206, 275, 314, 765, 2732
FbaI	TGATCA	4 cut(s)	442, 1261, 2103, 2886
FblI	GTMKAC	4 cut(s)	1777, 2208, 2409, 2880
Fnu4HI	GCNGC	7 cut(s)	66, 143, 151, 280, 304, 777, 2308
FokI	GGATG	9 cut(s)	85, 366, 503, 780, 836, 1232, 1583, 2789, 3504
Fsp4HI	GCNGC	7 cut(s)	66, 143, 151, 280, 304, 777, 2308
GlaI	GCGC	8 cut(s)	164, 166, 262, 270, 296, 1908, 2311, 3373
GluI	GCNGC	7 cut(s)	66, 143, 151, 280, 304, 777, 2308
GsaI	CCCAGC	3 cut(s)	162, 315, 3321
GsuI	CTGGAG	2 cut(s)	136, 2168
HaeII	RGCGCY	3 cut(s)	1910, 2313, 3375
HaeIII	GGCC	7 cut(s)	150, 350, 1684, 2321, 3309, 3362, 3387
HapII	CCGG	7 cut(s)	471, 487, 665, 2838, 3056, 3131, 3436
HgaI	GACGC	4 cut(s)	20, 232, 1031, 1571
HhaI	GCGC	8 cut(s)	165, 167, 263, 271, 297, 1909, 2312, 3374
Hin1I	GRCGYC	3 cut(s)	909, 1582, 3372
Hin6I	GCGC	8 cut(s)	163, 165, 261, 269, 295, 1907, 2310, 3372
HinP1I	GCGC	8 cut(s)	163, 165, 261, 269, 295, 1907, 2310, 3372
HincII	GTYRAC	2 cut(s)	466, 2881
HindII	GTYRAC	2 cut(s)	466, 2881
HindIII	AAGCTT	3 cut(s)	530, 2249, 3406
HpaII	CCGG	7 cut(s)	471, 487, 665, 2838, 3056, 3131, 3436
Hpy166II	GTNNAC	7 cut(s)	466, 1659, 1778, 2209, 2410, 2881, 3203
Hpy8I	GTNNAC	7 cut(s)	466, 1659, 1778, 2209, 2410, 2881, 3203
Hpy99I	CGWCG	6 cut(s)	754, 894, 911, 3033, 3275, 3371
HpyAV	CCTTC	9 cut(s)	1222, 1337, 1424, 1492, 1738, 2370, 2774, 3083, 3128
HpyCH4IV	ACGT	5 cut(s)	678, 909, 932, 1913, 2059
HpySE526I	ACGT	5 cut(s)	678, 909, 932, 1913, 2059
Hsp92I	GRCGYC	3 cut(s)	909, 1582, 3372
HspAI	GCGC	8 cut(s)	163, 165, 261, 269, 295, 1907, 2310, 3372
KasI	GGCGCC	1 cut(s)	3371
Kpn2I	TCCGGA	1 cut(s)	3130
Ksp22I	TGATCA	4 cut(s)	442, 1261, 2103, 2886
KspI	CCGCGG	1 cut(s)	154
LguI	GCTCTTC	1 cut(s)	3433
LmnI	GCTCC	6 cut(s)	117, 258, 276, 1598, 2439, 3438
Lsp1109I	GCAGC	6 cut(s)	52, 129, 266, 290, 788, 2319
LweI	GCATC	4 cut(s)	952, 1444, 2142, 2856
MabI	ACCWGGT	1 cut(s)	2912
MaeII	ACGT	5 cut(s)	678, 909, 932, 1913, 2059
MbiI	CCGCTC	1 cut(s)	2739
MflI	RGATCY	4 cut(s)	796, 1123, 1419, 1477
MhlI	GDGCHC	5 cut(s)	837, 900, 1603, 1926, 2966
MlsI	TGGCCA	1 cut(s)	3387
MluNI	TGGCCA	1 cut(s)	3387
Mly113I	GGCGCC	1 cut(s)	3372
MlyI	GAGTC	4 cut(s)	1199, 1365, 1448, 2918
MmeI	TCCRAC	5 cut(s)	34, 732, 2461, 2743, 3052
Mox20I	TGGCCA	1 cut(s)	3387
Mph1103I	ATGCAT	1 cut(s)	1652
MroI	TCCGGA	1 cut(s)	3130
MroXI	GAANNNNTTC	1 cut(s)	3126
MscI	TGGCCA	1 cut(s)	3387
MslI	CAYNNNNRTG	4 cut(s)	1168, 1387, 2385, 3560
Msp20I	TGGCCA	1 cut(s)	3387
MspA1I	CMGCKG	1 cut(s)	153
MspI	CCGG	7 cut(s)	471, 487, 665, 2838, 3056, 3131, 3436
MspR9I	CCNGG	6 cut(s)	488, 914, 1177, 2496, 2839, 2914
Mva1269I	GAATGC	2 cut(s)	3196, 3284
MvaI	CCWGG	4 cut(s)	914, 1177, 2496, 2914
MvnI	CGCG	5 cut(s)	49, 153, 165, 185, 271
NarI	GGCGCC	1 cut(s)	3372
NciI	CCSGG	2 cut(s)	488, 2839
NcoI	CCATGG	1 cut(s)	3487
NheI	GCTAGC	3 cut(s)	103, 226, 236
NlaIV	GGNNCC	4 cut(s)	1089, 2656, 3373, 3515
NmeAIII	GCCGAG	1 cut(s)	991
NmuCI	GTSAC	7 cut(s)	552, 994, 1621, 2055, 2672, 2908, 2916
NsiI	ATGCAT	1 cut(s)	1652
NspI	RCATGY	5 cut(s)	195, 1392, 1650, 1727, 3002
NspV	TTCGAA	2 cut(s)	1789, 3403
PaeI	GCATGC	2 cut(s)	195, 1650
PaqCI	CACCTGC	1 cut(s)	1730
PasI	CCCWGGG	1 cut(s)	1176
PauI	GCGCGC	1 cut(s)	163
PceI	AGGCCT	1 cut(s)	350
PciSI	GCTCTTC	1 cut(s)	3433
PcsI	WCGNNNNNNNCGW	3 cut(s)	963, 3270, 3279
PctI	GAATGC	2 cut(s)	3196, 3284
PdmI	GAANNNNTTC	1 cut(s)	3126
PfeI	GAWTC	8 cut(s)	724, 839, 1288, 1946, 2487, 2724, 2941, 3141
PflFI	GACNNNGTC	1 cut(s)	1025
PflMI	CCANNNNNTGG	1 cut(s)	3452
PfoI	TCCNGGA	3 cut(s)	486, 912, 2837
PinAI	ACCGGT	1 cut(s)	664
PkrI	GCNGC	7 cut(s)	67, 144, 152, 281, 305, 778, 2309
PleI	GAGTC	4 cut(s)	1199, 1364, 1447, 2918
PluTI	GGCGCC	1 cut(s)	3375
PpsI	GAGTC	4 cut(s)	1199, 1364, 1447, 2918
PpuMI	RGGWCCY	1 cut(s)	1042
PshAI	GACNNNNGTC	1 cut(s)	908
PshBI	ATTAAT	1 cut(s)	1166
Psp5II	RGGWCCY	1 cut(s)	1042
Psp6I	CCWGG	4 cut(s)	912, 1175, 2494, 2912
PspEI	GGTNACC	1 cut(s)	3456
PspFI	CCCAGC	3 cut(s)	158, 311, 3317
PspGI	CCWGG	4 cut(s)	912, 1175, 2494, 2912
PspN4I	GGNNCC	4 cut(s)	1089, 2656, 3373, 3515
PspPI	GGNCC	5 cut(s)	148, 1042, 2320, 3307, 3540
PspPPI	RGGWCCY	1 cut(s)	1042
PsrI	GAACNNNNNNTAC	2 cut(s)	1173, 1205
PstI	CTGCAG	2 cut(s)	147, 308
PstNI	CAGNNNCTG	1 cut(s)	303
PsuI	RGATCY	4 cut(s)	796, 1123, 1419, 1477
PsyI	GACNNNGTC	1 cut(s)	1025
PteI	GCGCGC	1 cut(s)	163
RsaI	GTAC	5 cut(s)	1119, 1660, 1976, 2134, 3202
RsaNI	GTAC	5 cut(s)	1118, 1659, 1975, 2133, 3201
RseI	CAYNNNNRTG	4 cut(s)	1168, 1387, 2385, 3560
SacII	CCGCGG	1 cut(s)	154
SalI	GTCGAC	1 cut(s)	2879
SapI	GCTCTTC	1 cut(s)	3433
SatI	GCNGC	7 cut(s)	66, 143, 151, 280, 304, 777, 2308
Sau96I	GGNCC	5 cut(s)	148, 1042, 2320, 3307, 3540
SchI	GAGTC	4 cut(s)	1199, 1365, 1448, 2918
ScrFI	CCNGG	6 cut(s)	488, 914, 1177, 2496, 2839, 2914
SduI	GDGCHC	5 cut(s)	837, 900, 1603, 1926, 2966
SexAI	ACCWGGT	1 cut(s)	2912
SfaNI	GCATC	4 cut(s)	952, 1444, 2142, 2856
SfcI	CTRYAG	4 cut(s)	143, 196, 304, 2406
SfoI	GGCGCC	1 cut(s)	3373
Sfr303I	CCGCGG	1 cut(s)	154
SfuI	TTCGAA	2 cut(s)	1789, 3403
SgrBI	CCGCGG	1 cut(s)	154
SinI	GGWCC	2 cut(s)	1042, 3540
SmiMI	CAYNNNNRTG	4 cut(s)	1168, 1387, 2385, 3560
SmlI	CTYRAG	7 cut(s)	5, 403, 1874, 2110, 2426, 2549, 3350
SmoI	CTYRAG	7 cut(s)	5, 403, 1874, 2110, 2426, 2549, 3350
SphI	GCATGC	2 cut(s)	195, 1650
SseBI	AGGCCT	1 cut(s)	350
SspDI	GGCGCC	1 cut(s)	3371
SspI	AATATT	1 cut(s)	1898
StuI	AGGCCT	1 cut(s)	350
StyD4I	CCNGG	6 cut(s)	486, 912, 1175, 2494, 2837, 2912
StyI	CCWWGG	1 cut(s)	3487
TaiI	ACGT	5 cut(s)	681, 912, 935, 1916, 2062
TaqII	GACCGA	1 cut(s)	920
TatI	WGTACW	2 cut(s)	1658, 3200
TauI	GCSGC	1 cut(s)	153
TfiI	GAWTC	8 cut(s)	724, 839, 1288, 1946, 2487, 2724, 2941, 3141
TscAI	CASTG	5 cut(s)	445, 999, 1567, 2224, 2992
TseFI	GTSAC	7 cut(s)	552, 994, 1621, 2055, 2672, 2908, 2916
TseI	GCWGC	6 cut(s)	65, 142, 279, 303, 776, 2307
Tsp45I	GTSAC	7 cut(s)	552, 994, 1621, 2055, 2672, 2908, 2916
TspDTI	ATGAA	8 cut(s)	467, 837, 838, 1256, 1453, 1484, 2793, 3227
TspGWI	ACGGA	1 cut(s)	3029
TspRI	CASTG	5 cut(s)	445, 999, 1567, 2224, 2992
Tth111I	GACNNNGTC	1 cut(s)	1025
Van91I	CCANNNNNTGG	1 cut(s)	3452
VpaK11BI	GGWCC	2 cut(s)	1042, 3540
VspI	ATTAAT	1 cut(s)	1166
XagI	CCTNNNNNAGG	1 cut(s)	1049
XapI	RAATTY	4 cut(s)	1403, 1431, 2019, 2257
XceI	RCATGY	5 cut(s)	195, 1392, 1650, 1727, 3002
XcmI	CCANNNNNNNNNTGG	1 cut(s)	604
XmiI	GTMKAC	4 cut(s)	1777, 2208, 2409, 2880
XmnI	GAANNNNTTC	1 cut(s)	3126
ZraI	GACGTC	1 cut(s)	910
Zsp2I	ATGCAT	1 cut(s)	1652

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.