MD02G1275200.v1.1

ATP-dependent Clp protease proteolytic subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0003024
Physical Location & Seq
Forward (+)
32942090 .. 32947352
5263 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1275200.v1.1.491

Sequence Viewer

Length: 966 bp
ATGATGACGTACGCAGCCACAGCTTCAAGGCCGCCGTGTTCTTCACAAACCCAAGTGCCCATTTCACCGATTGTGAGGGGCACTGCCCTCAACAGCAGCAGCAGCAGCTGCAGTGGTATTACCAGTTGCCGTCGGAGAAGCAGTGGCGTCTGTGTAATTAAGGCCATGAAAAGCTCGTCTCCCCCAAGACTAACCTTGTCGAGCAACTGGGATTTTCCGTCAGCTACTACTTCGGCTCCAAGGCTGCCCAGATTCGAAGAACTCGATACCACCAACATGCTTCTCCGTCAGAGAATCATCTTTCTGGGTTCTCAGGTAGATGACATGACCGCGGATTTGATCATAAGCCAACTTCTATTTCTAGATGCTGAGGACCCCAAAAAGGACATTAAATTGTTTATCAATTCACCCGGTGGTTCTGTTACTGCTGGAATGGGAATATATGATGCAATGAAATTGTGCAAGGCAGATGTTTCAACTGTTTGCCTGGGGCTTGCTGCTTCTATGGGGGCCTTTCTCCTTGCTGCAGGCTCTAAGGGAAAGAGATTCTGCATGCCCAATGCAAGAGTGATGATCCATCAACCCCTTGGAACTGCAGGAGGAAAAGCAACGGAAATGGGTATTCGAATCAGAGAAATGGTGTACCACAAGATTAAATTGAACAAAATATTTTCAAGAATCACAGGGAAGCCTTTAGAGCAGATTGAAGAGGACACAGACCGTGATAATTTCATGAATCCTTGGGAAGCTAAGGATTACGGGTTGATTGATTTTGTTATCGATGATGGAAAGCCAGGCTTAGTTGCGCCCGTTGGAGATTTCACACCTCCACCAAGAACACGAGTCTGGGATCAATGGAAAGTCGAAGGGAGCAAGAAATCGAGAAAGAACTTGCCCTCAGAGCAAAGATTTTCTGACAATGGAAGCGGGGCTGAGAAGGAAAAGGAAGCACCCACTGCCATATGA

Protein Analysis

322

Amino Acids

34.99

Weight (kDa)

9.12

Isoelectric Point (pI)

41.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLP_protease PF00574 88 - 261 1.3e-75 Clp protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 332
AciI CCGC 4 cut(s) 32, 330, 332, 927
AclWI GGATC 2 cut(s) 568, 858
AcyI GRCGYC 1 cut(s) 147
AdeI CACNNNGTG 1 cut(s) 413
AfaI GTAC 2 cut(s) 11, 644
AfiI CCNNNNNNNGG 1 cut(s) 382
AgsI TTSAA 5 cut(s) 27, 477, 661, 675, 707
AjnI CCWGG 2 cut(s) 486, 793
AjuI GAANNNNNNNTTGG 2 cut(s) 342, 374
AluBI AGCT 5 cut(s) 23, 108, 174, 224, 749
AluI AGCT 5 cut(s) 23, 108, 174, 224, 749
Alw26I GTCTC 1 cut(s) 183
AlwI GGATC 2 cut(s) 568, 858
AlwNI CAGNNNCTG 1 cut(s) 108
AoxI GGCC 3 cut(s) 29, 162, 510
ApeKI GCWGC 9 cut(s) 14, 96, 99, 102, 105, 108, 244, 497, 524
ArsI GACNNNNNNTTYG 2 cut(s) 319, 351
AspLEI GCGC 1 cut(s) 808
AspS9I GGNCC 2 cut(s) 373, 510
AsuC2I CCSGG 1 cut(s) 411
AsuHPI GGTGA 2 cut(s) 57, 399
AsuII TTCGAA 2 cut(s) 255, 625
AvaII GGWCC 1 cut(s) 373
BaeGI GKGCMC 2 cut(s) 60, 83
BauI CACGAG 1 cut(s) 840
BbvCI CCTCAGC 1 cut(s) 369
BbvI GCAGC 9 cut(s) 26, 95, 108, 111, 114, 117, 231, 484, 511
BccI CCATC 2 cut(s) 585, 779
BceAI ACGGC 2 cut(s) 19, 114
BciT130I CCWGG 2 cut(s) 488, 795
BclI TGATCA 1 cut(s) 339
BcnI CCSGG 1 cut(s) 411
BcoDI GTCTC 1 cut(s) 183
BfaI CTAG 1 cut(s) 362
BfmI CTRYAG 3 cut(s) 109, 525, 594
Bme1390I CCNGG 3 cut(s) 411, 488, 795
Bme18I GGWCC 1 cut(s) 373
BmgT120I GGNCC 2 cut(s) 373, 510
BmiI GGNNCC 3 cut(s) 237, 375, 511
BmrFI CCNGG 3 cut(s) 411, 488, 795
BmrI ACTGGG 1 cut(s) 217
BmsI GCATC 2 cut(s) 355, 436
BmuI ACTGGG 1 cut(s) 217
Bpu10I CCTNAGC 2 cut(s) 369, 750
Bpu14I TTCGAA 2 cut(s) 255, 625
BpuMI CCSGG 1 cut(s) 411
Bsa29I ATCGAT 1 cut(s) 780
BsaHI GRCGYC 1 cut(s) 147
BsaJI CCNNGG 5 cut(s) 239, 330, 487, 586, 740
BsaXI ACNNNNNCTCC 4 cut(s) 127, 157, 220, 250
Bsc4I CCNNNNNNNGG 1 cut(s) 382
Bse1I ACTGG 2 cut(s) 123, 212
Bse3DI GCAATG 1 cut(s) 456
BseBI CCWGG 2 cut(s) 488, 795
BseCI ATCGAT 1 cut(s) 780
BseDI CCNNGG 5 cut(s) 239, 330, 487, 586, 740
BseLI CCNNNNNNNGG 1 cut(s) 382
BseMI GCAATG 1 cut(s) 456
BseMII CTCAG 4 cut(s) 326, 360, 912, 924
BseNI ACTGG 2 cut(s) 123, 212
BseSI GKGCMC 2 cut(s) 60, 83
BseXI GCAGC 9 cut(s) 26, 95, 108, 111, 114, 117, 231, 484, 511
Bsh1236I CGCG 1 cut(s) 332
BshFI GGCC 3 cut(s) 31, 164, 512
BshVI ATCGAT 1 cut(s) 780
BsiSI CCGG 1 cut(s) 411
BsiWI CGTACG 1 cut(s) 9
BslI CCNNNNNNNGG 1 cut(s) 382
BsmAI GTCTC 1 cut(s) 183
BsmBI CGTCTC 1 cut(s) 183
BsnI GGCC 3 cut(s) 31, 164, 512
Bsp119I TTCGAA 2 cut(s) 255, 625
Bsp1286I GDGCHC 2 cut(s) 60, 83
Bsp143I GATC 3 cut(s) 339, 573, 850
BspACI CCGC 4 cut(s) 32, 330, 332, 927
BspANI GGCC 3 cut(s) 31, 164, 512
BspCNI CTCAG 4 cut(s) 325, 361, 911, 925
BspDI ATCGAT 1 cut(s) 780
BspFNI CGCG 1 cut(s) 332
BspHI TCATGA 1 cut(s) 732
BspLI GGNNCC 3 cut(s) 237, 375, 511
BspMAI CTGCAG 3 cut(s) 113, 529, 598
BspPI GGATC 2 cut(s) 568, 858
BspT104I TTCGAA 2 cut(s) 255, 625
BsrDI GCAATG 1 cut(s) 456
BsrI ACTGG 2 cut(s) 123, 212
BssECI CCNNGG 5 cut(s) 239, 330, 487, 586, 740
BssMI GATC 3 cut(s) 339, 573, 850
BssNI GRCGYC 1 cut(s) 147
BssSI CACGAG 1 cut(s) 840
BssT1I CCWWGG 3 cut(s) 239, 586, 740
Bst2BI CACGAG 1 cut(s) 840
Bst2UI CCWGG 2 cut(s) 488, 795
Bst4CI ACNGT 2 cut(s) 481, 722
Bst6I CTCTTC 1 cut(s) 702
BstACI GRCGYC 1 cut(s) 147
BstAPI GCANNNNNTGC 2 cut(s) 108, 956
BstBI TTCGAA 2 cut(s) 255, 625
BstC8I GCNNGC 3 cut(s) 495, 529, 554
BstDEI CTNAG 7 cut(s) 312, 369, 534, 750, 799, 898, 933
BstDSI CCRYGG 1 cut(s) 330
BstFNI CGCG 1 cut(s) 332
BstHHI GCGC 1 cut(s) 808
BstKTI GATC 3 cut(s) 342, 576, 853
BstMAI GTCTC 1 cut(s) 183
BstMBI GATC 3 cut(s) 339, 573, 850
BstMWI GCNNNNNNNGC 7 cut(s) 20, 102, 105, 108, 697, 901, 956
BstNI CCWGG 2 cut(s) 488, 795
BstNSI RCATGY 2 cut(s) 280, 556
BstSCI CCNGG 3 cut(s) 409, 486, 793
BstSFI CTRYAG 3 cut(s) 109, 525, 594
BstSLI GKGCMC 2 cut(s) 60, 83
BstUI CGCG 1 cut(s) 332
BstV1I GCAGC 9 cut(s) 26, 95, 108, 111, 114, 117, 231, 484, 511
Bsu15I ATCGAT 1 cut(s) 780
BsuRI GGCC 3 cut(s) 31, 164, 512
BsuTUI ATCGAT 1 cut(s) 780
BtgI CCRYGG 1 cut(s) 330
BtsI GCAGTG 4 cut(s) 81, 118, 148, 954
BtsIMutI CAGTG 4 cut(s) 81, 118, 148, 954
Cac8I GCNNGC 3 cut(s) 495, 529, 554
CaiI CAGNNNCTG 1 cut(s) 108
CciI TCATGA 1 cut(s) 732
CfoI GCGC 1 cut(s) 808
Cfr13I GGNCC 2 cut(s) 373, 510
Cfr42I CCGCGG 1 cut(s) 333
ClaI ATCGAT 1 cut(s) 780
CseI GACGC 1 cut(s) 136
Csp6I GTAC 2 cut(s) 10, 643
CviAII CATG 5 cut(s) 166, 277, 325, 553, 733
CviQI GTAC 2 cut(s) 10, 643
DdeI CTNAG 7 cut(s) 312, 369, 534, 750, 799, 898, 933
DpnI GATC 3 cut(s) 341, 575, 852
DpnII GATC 3 cut(s) 339, 573, 850
DraIII CACNNNGTG 1 cut(s) 413
Eam1104I CTCTTC 1 cut(s) 702
EarI CTCTTC 1 cut(s) 702
Eco130I CCWWGG 3 cut(s) 239, 586, 740
Eco47I GGWCC 1 cut(s) 373
EcoO109I RGGNCCY 2 cut(s) 373, 510
EcoRII CCWGG 2 cut(s) 486, 793
EcoT14I CCWWGG 3 cut(s) 239, 586, 740
ErhI CCWWGG 3 cut(s) 239, 586, 740
Esp3I CGTCTC 1 cut(s) 183
FaeI CATG 5 cut(s) 169, 280, 328, 556, 736
FalI AAGNNNNNCTT 2 cut(s) 782, 814
FatI CATG 5 cut(s) 165, 276, 324, 552, 732
FauI CCCGC 1 cut(s) 920
FauNDI CATATG 1 cut(s) 962
FbaI TGATCA 1 cut(s) 339
FspBI CTAG 1 cut(s) 362
GlaI GCGC 1 cut(s) 807
HaeIII GGCC 3 cut(s) 31, 164, 512
HapII CCGG 1 cut(s) 411
HgaI GACGC 1 cut(s) 136
HhaI GCGC 1 cut(s) 808
Hin1I GRCGYC 1 cut(s) 147
Hin1II CATG 5 cut(s) 169, 280, 328, 556, 736
Hin6I GCGC 1 cut(s) 806
HinP1I GCGC 1 cut(s) 806
HinfI GANTC 7 cut(s) 252, 294, 546, 627, 678, 736, 843
HpaII CCGG 1 cut(s) 411
HphI GGTGA 2 cut(s) 57, 399
Hpy166II GTNNAC 1 cut(s) 643
Hpy188I TCNGA 5 cut(s) 135, 291, 632, 901, 916
Hpy188III TCNNGA 4 cut(s) 362, 675, 733, 882
Hpy8I GTNNAC 1 cut(s) 643
Hpy99I CGWCG 1 cut(s) 135
HpyAV CCTTC 2 cut(s) 860, 931
HpyCH4III ACNGT 2 cut(s) 481, 722
HpyCH4IV ACGT 1 cut(s) 8
HpyCH4V TGCA 7 cut(s) 111, 449, 462, 527, 552, 563, 596
HpyF10VI GCNNNNNNNGC 7 cut(s) 20, 102, 105, 108, 697, 901, 956
HpyF3I CTNAG 7 cut(s) 312, 369, 534, 750, 799, 898, 933
HpySE526I ACGT 1 cut(s) 8
Hsp92I GRCGYC 1 cut(s) 147
Hsp92II CATG 5 cut(s) 169, 280, 328, 556, 736
HspAI GCGC 1 cut(s) 806
Ksp22I TGATCA 1 cut(s) 339
KspI CCGCGG 1 cut(s) 333
Kzo9I GATC 3 cut(s) 339, 573, 850
LmnI GCTCC 2 cut(s) 241, 870
Lsp1109I GCAGC 9 cut(s) 26, 95, 108, 111, 114, 117, 231, 484, 511
LweI GCATC 2 cut(s) 355, 436
MaeI CTAG 1 cut(s) 362
MaeII ACGT 1 cut(s) 8
MaeIII GTNAC 1 cut(s) 421
MalI GATC 3 cut(s) 341, 575, 852
MboI GATC 3 cut(s) 339, 573, 850
MboII GAAGA 3 cut(s) 33, 269, 719
MhlI GDGCHC 2 cut(s) 60, 83
MluCI AATT 6 cut(s) 156, 392, 403, 455, 656, 727
MlyI GAGTC 1 cut(s) 852
MmeI TCCRAC 2 cut(s) 113, 793
MnlI CCTC 7 cut(s) 69, 98, 364, 593, 703, 837, 907
MseI TTAA 3 cut(s) 159, 390, 654
MslI CAYNNNNRTG 1 cut(s) 275
MspA1I CMGCKG 2 cut(s) 108, 332
MspI CCGG 1 cut(s) 411
MspR9I CCNGG 3 cut(s) 411, 488, 795
MvaI CCWGG 2 cut(s) 488, 795
MvnI CGCG 1 cut(s) 332
MwoI GCNNNNNNNGC 7 cut(s) 20, 102, 105, 108, 697, 901, 956
NciI CCSGG 1 cut(s) 411
NdeI CATATG 1 cut(s) 962
NdeII GATC 3 cut(s) 339, 573, 850
NlaIII CATG 5 cut(s) 169, 280, 328, 556, 736
NlaIV GGNNCC 3 cut(s) 237, 375, 511
NspI RCATGY 2 cut(s) 280, 556
NspV TTCGAA 2 cut(s) 255, 625
PaeI GCATGC 1 cut(s) 556
PagI TCATGA 1 cut(s) 732
PcsI WCGNNNNNNNCGW 1 cut(s) 261
PfeI GAWTC 6 cut(s) 252, 294, 546, 627, 678, 736
Pfl23II CGTACG 1 cut(s) 9
PleI GAGTC 1 cut(s) 851
PpsI GAGTC 1 cut(s) 851
PpuMI RGGWCCY 1 cut(s) 373
Psp5II RGGWCCY 1 cut(s) 373
Psp6I CCWGG 2 cut(s) 486, 793
PspGI CCWGG 2 cut(s) 486, 793
PspLI CGTACG 1 cut(s) 9
PspN4I GGNNCC 3 cut(s) 237, 375, 511
PspPI GGNCC 2 cut(s) 373, 510
PspPPI RGGWCCY 1 cut(s) 373
PstI CTGCAG 3 cut(s) 113, 529, 598
PstNI CAGNNNCTG 1 cut(s) 108
PvuII CAGCTG 1 cut(s) 108
RsaI GTAC 2 cut(s) 11, 644
RsaNI GTAC 2 cut(s) 10, 643
RseI CAYNNNNRTG 1 cut(s) 275
SacII CCGCGG 1 cut(s) 333
SaqAI TTAA 3 cut(s) 159, 390, 654
Sau3AI GATC 3 cut(s) 339, 573, 850
Sau96I GGNCC 2 cut(s) 373, 510
SchI GAGTC 1 cut(s) 852
ScrFI CCNGG 3 cut(s) 411, 488, 795
SduI GDGCHC 2 cut(s) 60, 83
SetI ASST 9 cut(s) 11, 25, 110, 176, 197, 226, 318, 751, 829
SfaNI GCATC 2 cut(s) 355, 436
SfcI CTRYAG 3 cut(s) 109, 525, 594
Sfr303I CCGCGG 1 cut(s) 333
SfuI TTCGAA 2 cut(s) 255, 625
SgrBI CCGCGG 1 cut(s) 333
SinI GGWCC 1 cut(s) 373
SmiMI CAYNNNNRTG 1 cut(s) 275
SphI GCATGC 1 cut(s) 556
Sse9I AATT 6 cut(s) 156, 392, 403, 455, 656, 727
SsiI CCGC 4 cut(s) 32, 330, 332, 927
SspI AATATT 1 cut(s) 669
SspMI CTAG 1 cut(s) 362
StyD4I CCNGG 3 cut(s) 409, 486, 793
StyI CCWWGG 3 cut(s) 239, 586, 740
TaaI ACNGT 2 cut(s) 481, 722
TaiI ACGT 1 cut(s) 11
TaqI TCGA 7 cut(s) 200, 255, 264, 625, 780, 864, 881
TasI AATT 6 cut(s) 156, 392, 403, 455, 656, 727
TauI GCSGC 1 cut(s) 34
TfiI GAWTC 6 cut(s) 252, 294, 546, 627, 678, 736
Tru1I TTAA 3 cut(s) 159, 390, 654
Tru9I TTAA 3 cut(s) 159, 390, 654
TscAI CASTG 4 cut(s) 88, 118, 148, 961
TseI GCWGC 9 cut(s) 14, 96, 99, 102, 105, 108, 244, 497, 524
TspDTI ATGAA 4 cut(s) 182, 467, 721, 749
TspGWI ACGGA 3 cut(s) 207, 275, 626
TspRI CASTG 4 cut(s) 88, 118, 148, 961
VpaK11BI GGWCC 1 cut(s) 373
XbaI TCTAGA 1 cut(s) 361
XceI RCATGY 2 cut(s) 280, 556
XcmI CCANNNNNNNNNTGG 1 cut(s) 584
XspI CTAG 1 cut(s) 362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.