FvH4_7g04110

ATP-dependent Clp protease proteolytic subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0003024
Physical Location & Seq
Reverse (-)
4701340 .. 4708123
6784 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g04110.t1

Sequence Viewer

Length: 942 bp
ATGATGACGTACGCAGCCCCAGCGTCAAGGCCTCTGTGTTTCAACACCCAACCGTCGGTTGCTCTTCACAACGGTCTTCTCAGTGCGGCTACTAGTACTAGTAGTACCATCGGTTGTAGCAGAAGCTCAAGGAAGGTCGTGGGTTCAGTTAAAGCCTCGTCGTCTCCCACAAGACCCACCTTGTCCACCAATTGGGATTTCCAGTTCCCCGCTGCTACTGCCCCGTCTCCTACACGCGTCCCCAGATTCGAAGAGCTCGACACCACCAACATGCTCCTCCGCCAGAGAATCATCTTCTTGGGCTCTCAGGTGGATGACATGACGGCGGATTTGATCATAAGCCAGCTGCTGTTTCTGGATGCTGAAGACCCCCAAAAGGACATTACATTGTTTATCAATTCACCCGGTGGCTCGGTTACGGCTGGAATGGGAATATATGATGCAATGAAAATGTGCAAGGCAGATGTTTCAACTGTTTGCTTCGGGCTTGCTGCTTCAATGGGTGCATTTCTCCTTGCTTCCGGCTCAAAAGGAAAGAGGTACTGCATGCCTAATGCAAGAGTGATGATCCATCAACCTCTTGGAACTGCTGGAGGCAAAGCAACAGAAATGGGTATACGAATTAGAGAAATGGCATACCACAAGATTAAATTGAACAAGATATTGTCAAGAATCACAGGAAAGCCGTTTGAACAGGTTGAGGAGGATACAGACCGTGATAATTTCATGAATCCCTGGGAAGCTAAGGATTATGGGTTGATTGATGATGTTATTGATGATGGAAAGCCAGGGTTGGTTGCACCAATTGGAGATTCCACACCCCCACCTAGAACAAAAGTCTGGGATATGTGGAAAGTAGAAGGTACCAAGAAATCCAGAAAGAATTTACCCTCAGAGCAGAAAAATTTAGAGAAAGGTTTGGAAGCACCTACTGCAACATAG

Protein Analysis

314

Amino Acids

34.03

Weight (kDa)

8.72

Isoelectric Point (pI)

39.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLP_protease PF00574 86 - 259 1.2e-75 Clp protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 863
AccB1I GGYRCC 1 cut(s) 863
AccB7I CCANNNNNTGG 1 cut(s) 192
AccI GTMKAC 1 cut(s) 616
AccII CGCG 1 cut(s) 237
AciI CCGC 4 cut(s) 86, 210, 280, 326
AclWI GGATC 1 cut(s) 562
AcsI RAATTY 2 cut(s) 883, 904
AcuI CTGAAG 1 cut(s) 384
AdeI CACNNNGTG 1 cut(s) 407
AfaI GTAC 5 cut(s) 11, 97, 106, 542, 865
AfiI CCNNNNNNNGG 3 cut(s) 55, 192, 376
AflIII ACRYGT 1 cut(s) 235
AgsI TTSAA 5 cut(s) 43, 471, 498, 655, 692
AhlI ACTAGT 2 cut(s) 92, 98
AjnI CCWGG 2 cut(s) 734, 787
AjuI GAANNNNNNNTTGG 4 cut(s) 182, 214, 796, 828
AloI GAACNNNNNNTCC 2 cut(s) 188, 220
AluBI AGCT 4 cut(s) 126, 256, 346, 743
AluI AGCT 4 cut(s) 126, 256, 346, 743
Alw21I GWGCWC 1 cut(s) 258
Alw26I GTCTC 2 cut(s) 168, 231
AlwI GGATC 1 cut(s) 562
AlwNI CAGNNNCTG 1 cut(s) 349
AoxI GGCC 1 cut(s) 29
ApeKI GCWGC 4 cut(s) 14, 212, 346, 491
ApoI RAATTY 2 cut(s) 883, 904
ArsI GACNNNNNNTTYG 2 cut(s) 313, 345
Asp718I GGTACC 1 cut(s) 863
AsuC2I CCSGG 1 cut(s) 405
AsuHPI GGTGA 1 cut(s) 393
AsuII TTCGAA 1 cut(s) 249
BanI GGYRCC 1 cut(s) 863
BanII GRGCYC 2 cut(s) 258, 305
BbsI GAAGAC 2 cut(s) 68, 372
Bbv12I GWGCWC 1 cut(s) 258
BbvI GCAGC 4 cut(s) 26, 199, 333, 478
BccI CCATC 3 cut(s) 116, 579, 773
BceAI ACGGC 3 cut(s) 339, 435, 670
BciT130I CCWGG 2 cut(s) 736, 789
BciVI GTATCC 1 cut(s) 700
BclI TGATCA 1 cut(s) 333
BcnI CCSGG 1 cut(s) 405
BcoDI GTCTC 2 cut(s) 168, 231
BcuI ACTAGT 2 cut(s) 92, 98
BfaI CTAG 3 cut(s) 93, 99, 828
BfuI GTATCC 1 cut(s) 700
BisI GCNGC 5 cut(s) 15, 87, 213, 347, 492
BlsI GCNGC 5 cut(s) 16, 88, 214, 348, 493
BmcAI AGTACT 1 cut(s) 97
Bme1390I CCNGG 3 cut(s) 405, 736, 789
BmiI GGNNCC 1 cut(s) 865
BmrFI CCNGG 3 cut(s) 405, 736, 789
BmsI GCATC 2 cut(s) 349, 430
BpiI GAAGAC 2 cut(s) 68, 372
BpmI CTGGAG 1 cut(s) 612
Bpu10I CCTNAGC 1 cut(s) 744
Bpu14I TTCGAA 1 cut(s) 249
BpuEI CTTGAG 1 cut(s) 112
BpuMI CCSGG 1 cut(s) 405
BsaJI CCNNGG 3 cut(s) 734, 735, 788
Bsc4I CCNNNNNNNGG 3 cut(s) 55, 192, 376
Bse1I ACTGG 1 cut(s) 202
Bse3DI GCAATG 1 cut(s) 450
BseBI CCWGG 2 cut(s) 736, 789
BseDI CCNNGG 3 cut(s) 734, 735, 788
BseGI GGATG 2 cut(s) 319, 364
BseLI CCNNNNNNNGG 3 cut(s) 55, 192, 376
BseMI GCAATG 1 cut(s) 450
BseMII CTCAG 3 cut(s) 94, 320, 906
BseNI ACTGG 1 cut(s) 202
BseRI GAGGAG 2 cut(s) 266, 716
BseXI GCAGC 4 cut(s) 26, 199, 333, 478
BseYI CCCAGC 1 cut(s) 19
Bsh1236I CGCG 1 cut(s) 237
BshFI GGCC 1 cut(s) 31
BshNI GGYRCC 1 cut(s) 863
BsiHKAI GWGCWC 1 cut(s) 258
BsiSI CCGG 2 cut(s) 405, 522
BsiWI CGTACG 1 cut(s) 9
BslFI GGGAC 1 cut(s) 224
BslI CCNNNNNNNGG 3 cut(s) 55, 192, 376
BsmAI GTCTC 2 cut(s) 168, 231
BsmBI CGTCTC 2 cut(s) 168, 231
BsmFI GGGAC 1 cut(s) 224
BsnI GGCC 1 cut(s) 31
Bsp119I TTCGAA 1 cut(s) 249
Bsp1286I GDGCHC 2 cut(s) 258, 305
Bsp143I GATC 2 cut(s) 333, 567
BspACI CCGC 4 cut(s) 86, 210, 280, 326
BspANI GGCC 1 cut(s) 31
BspCNI CTCAG 3 cut(s) 93, 319, 905
BspFNI CGCG 1 cut(s) 237
BspHI TCATGA 1 cut(s) 726
BspLI GGNNCC 1 cut(s) 865
BspPI GGATC 1 cut(s) 562
BspQI GCTCTTC 2 cut(s) 69, 246
BspT104I TTCGAA 1 cut(s) 249
BspT107I GGYRCC 1 cut(s) 863
BsrDI GCAATG 1 cut(s) 450
BsrI ACTGG 1 cut(s) 202
BssECI CCNNGG 3 cut(s) 734, 735, 788
BssMI GATC 2 cut(s) 333, 567
BssNAI GTATAC 1 cut(s) 617
Bst1107I GTATAC 1 cut(s) 617
Bst2UI CCWGG 2 cut(s) 736, 789
Bst4CI ACNGT 4 cut(s) 54, 74, 475, 716
Bst6I CTCTTC 2 cut(s) 69, 246
BstAPI GCANNNNNTGC 1 cut(s) 932
BstBI TTCGAA 1 cut(s) 249
BstC8I GCNNGC 3 cut(s) 344, 489, 548
BstDEI CTNAG 4 cut(s) 80, 306, 744, 892
BstF5I GGATG 2 cut(s) 319, 364
BstFNI CGCG 1 cut(s) 237
BstKTI GATC 2 cut(s) 336, 570
BstMAI GTCTC 2 cut(s) 168, 231
BstMBI GATC 2 cut(s) 333, 567
BstMWI GCNNNNNNNGC 3 cut(s) 20, 218, 932
BstNI CCWGG 2 cut(s) 736, 789
BstNSI RCATGY 2 cut(s) 274, 550
BstSCI CCNGG 3 cut(s) 403, 734, 787
BstUI CGCG 1 cut(s) 237
BstV1I GCAGC 4 cut(s) 26, 199, 333, 478
BstV2I GAAGAC 2 cut(s) 68, 372
BstZ17I GTATAC 1 cut(s) 617
BsuI GTATCC 1 cut(s) 700
BsuRI GGCC 1 cut(s) 31
BtsCI GGATG 2 cut(s) 319, 364
BtsIMutI CAGTG 1 cut(s) 88
Cac8I GCNNGC 3 cut(s) 344, 489, 548
CaiI CAGNNNCTG 1 cut(s) 349
CciI TCATGA 1 cut(s) 726
CseI GACGC 2 cut(s) 12, 226
Csp6I GTAC 5 cut(s) 10, 96, 105, 541, 864
CviAII CATG 4 cut(s) 271, 319, 547, 727
CviQI GTAC 5 cut(s) 10, 96, 105, 541, 864
DdeI CTNAG 4 cut(s) 80, 306, 744, 892
DpnI GATC 2 cut(s) 335, 569
DpnII GATC 2 cut(s) 333, 567
DraIII CACNNNGTG 1 cut(s) 407
Eam1104I CTCTTC 2 cut(s) 69, 246
EarI CTCTTC 2 cut(s) 69, 246
EciI GGCGGA 2 cut(s) 269, 341
Ecl136II GAGCTC 1 cut(s) 256
Eco147I AGGCCT 1 cut(s) 31
Eco24I GRGCYC 2 cut(s) 258, 305
Eco53kI GAGCTC 1 cut(s) 256
Eco57I CTGAAG 1 cut(s) 384
EcoICRI GAGCTC 1 cut(s) 256
EcoRII CCWGG 2 cut(s) 734, 787
EcoT38I GRGCYC 2 cut(s) 258, 305
Esp3I CGTCTC 2 cut(s) 168, 231
FaeI CATG 4 cut(s) 274, 322, 550, 730
FaqI GGGAC 1 cut(s) 224
FatI CATG 4 cut(s) 270, 318, 546, 726
FauI CCCGC 1 cut(s) 217
FbaI TGATCA 1 cut(s) 333
FblI GTMKAC 1 cut(s) 616
Fnu4HI GCNGC 5 cut(s) 15, 87, 213, 347, 492
FokI GGATG 2 cut(s) 326, 371
FriOI GRGCYC 2 cut(s) 258, 305
Fsp4HI GCNGC 5 cut(s) 15, 87, 213, 347, 492
FspBI CTAG 3 cut(s) 93, 99, 828
GluI GCNGC 5 cut(s) 15, 87, 213, 347, 492
GsaI CCCAGC 1 cut(s) 23
GsuI CTGGAG 1 cut(s) 612
HaeIII GGCC 1 cut(s) 31
HapII CCGG 2 cut(s) 405, 522
HgaI GACGC 2 cut(s) 12, 226
Hin1II CATG 4 cut(s) 274, 322, 550, 730
HinfI GANTC 5 cut(s) 246, 288, 672, 730, 812
HpaII CCGG 2 cut(s) 405, 522
HphI GGTGA 1 cut(s) 393
Hpy166II GTNNAC 2 cut(s) 186, 617
Hpy188I TCNGA 1 cut(s) 895
Hpy188III TCNNGA 4 cut(s) 356, 669, 727, 876
Hpy8I GTNNAC 2 cut(s) 186, 617
Hpy99I CGWCG 2 cut(s) 58, 163
HpyAV CCTTC 2 cut(s) 127, 854
HpyCH4III ACNGT 4 cut(s) 54, 74, 475, 716
HpyCH4IV ACGT 1 cut(s) 8
HpyCH4V TGCA 7 cut(s) 443, 456, 506, 546, 557, 800, 935
HpyF10VI GCNNNNNNNGC 3 cut(s) 20, 218, 932
HpyF3I CTNAG 4 cut(s) 80, 306, 744, 892
HpySE526I ACGT 1 cut(s) 8
Hsp92II CATG 4 cut(s) 274, 322, 550, 730
KpnI GGTACC 1 cut(s) 867
Ksp22I TGATCA 1 cut(s) 333
Kzo9I GATC 2 cut(s) 333, 567
LguI GCTCTTC 2 cut(s) 69, 246
LmnI GCTCC 1 cut(s) 279
Lsp1109I GCAGC 4 cut(s) 26, 199, 333, 478
LweI GCATC 2 cut(s) 349, 430
MaeI CTAG 3 cut(s) 93, 99, 828
MaeII ACGT 1 cut(s) 8
MaeIII GTNAC 1 cut(s) 415
MalI GATC 2 cut(s) 335, 569
MboI GATC 2 cut(s) 333, 567
MboII GAAGA 5 cut(s) 56, 68, 263, 286, 377
MfeI CAATTG 2 cut(s) 190, 804
MhlI GDGCHC 2 cut(s) 258, 305
MluCI AATT 8 cut(s) 190, 397, 621, 650, 721, 804, 883, 904
MluI ACGCGT 1 cut(s) 235
MnlI CCTC 9 cut(s) 42, 166, 287, 531, 587, 588, 694, 697, 901
MseI TTAA 2 cut(s) 150, 648
MslI CAYNNNNRTG 1 cut(s) 269
MspA1I CMGCKG 2 cut(s) 212, 346
MspI CCGG 2 cut(s) 405, 522
MspR9I CCNGG 3 cut(s) 405, 736, 789
MunI CAATTG 2 cut(s) 190, 804
MvaI CCWGG 2 cut(s) 736, 789
MvnI CGCG 1 cut(s) 237
MwoI GCNNNNNNNGC 3 cut(s) 20, 218, 932
NciI CCSGG 1 cut(s) 405
NdeII GATC 2 cut(s) 333, 567
NlaIII CATG 4 cut(s) 274, 322, 550, 730
NlaIV GGNNCC 1 cut(s) 865
NspI RCATGY 2 cut(s) 274, 550
NspV TTCGAA 1 cut(s) 249
PaeI GCATGC 1 cut(s) 550
PagI TCATGA 1 cut(s) 726
PasI CCCWGGG 1 cut(s) 735
PceI AGGCCT 1 cut(s) 31
PciSI GCTCTTC 2 cut(s) 69, 246
PcsI WCGNNNNNNNCGW 1 cut(s) 255
PfeI GAWTC 5 cut(s) 246, 288, 672, 730, 812
Pfl23II CGTACG 1 cut(s) 9
PflMI CCANNNNNTGG 1 cut(s) 192
PkrI GCNGC 5 cut(s) 16, 88, 214, 348, 493
Psp124BI GAGCTC 1 cut(s) 258
Psp6I CCWGG 2 cut(s) 734, 787
PspFI CCCAGC 1 cut(s) 19
PspGI CCWGG 2 cut(s) 734, 787
PspLI CGTACG 1 cut(s) 9
PspN4I GGNNCC 1 cut(s) 865
PstNI CAGNNNCTG 1 cut(s) 349
PvuII CAGCTG 1 cut(s) 346
RsaI GTAC 5 cut(s) 11, 97, 106, 542, 865
RsaNI GTAC 5 cut(s) 10, 96, 105, 541, 864
RseI CAYNNNNRTG 1 cut(s) 269
SacI GAGCTC 1 cut(s) 258
SapI GCTCTTC 2 cut(s) 69, 246
SaqAI TTAA 2 cut(s) 150, 648
SatI GCNGC 5 cut(s) 15, 87, 213, 347, 492
Sau3AI GATC 2 cut(s) 333, 567
ScaI AGTACT 1 cut(s) 97
ScrFI CCNGG 3 cut(s) 405, 736, 789
SduI GDGCHC 2 cut(s) 258, 305
SfaNI GCATC 2 cut(s) 349, 430
SfuI TTCGAA 1 cut(s) 249
SmiMI CAYNNNNRTG 1 cut(s) 269
SmlI CTYRAG 1 cut(s) 127
SmoI CTYRAG 1 cut(s) 127
SpeI ACTAGT 2 cut(s) 92, 98
SphI GCATGC 1 cut(s) 550
Sse9I AATT 8 cut(s) 190, 397, 621, 650, 721, 804, 883, 904
SseBI AGGCCT 1 cut(s) 31
SsiI CCGC 4 cut(s) 86, 210, 280, 326
SspMI CTAG 3 cut(s) 93, 99, 828
SstI GAGCTC 1 cut(s) 258
StuI AGGCCT 1 cut(s) 31
StyD4I CCNGG 3 cut(s) 403, 734, 787
TaaI ACNGT 4 cut(s) 54, 74, 475, 716
TaiI ACGT 1 cut(s) 11
TaqI TCGA 2 cut(s) 249, 258
TasI AATT 8 cut(s) 190, 397, 621, 650, 721, 804, 883, 904
TatI WGTACW 1 cut(s) 95
TauI GCSGC 1 cut(s) 89
TfiI GAWTC 5 cut(s) 246, 288, 672, 730, 812
Tru1I TTAA 2 cut(s) 150, 648
Tru9I TTAA 2 cut(s) 150, 648
TscAI CASTG 1 cut(s) 88
TseI GCWGC 4 cut(s) 14, 212, 346, 491
TspDTI ATGAA 3 cut(s) 461, 715, 743
TspRI CASTG 1 cut(s) 88
Van91I CCANNNNNTGG 1 cut(s) 192
XapI RAATTY 2 cut(s) 883, 904
XceI RCATGY 2 cut(s) 274, 550
XcmI CCANNNNNNNNNTGG 1 cut(s) 578
XmiI GTMKAC 1 cut(s) 616
XspI CTAG 3 cut(s) 93, 99, 828
ZrmI AGTACT 1 cut(s) 97
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.