MD07G1043100.v1.1

ATP-dependent Clp protease proteolytic subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0003024
Physical Location & Seq
Forward (+)
3602905 .. 3610925
8021 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1043100.v1.1.491

Sequence Viewer

Length: 951 bp
ATGATGACGTACGCAGCCACAGCTTCAAGGCCGCTGTGTTCTTCACAAACCCAAGTGCCCATTTCGCCAATTGTGAAGGGCACTGCCCTCAACAGCAATGGTATTATCAATTGCCATAGGAGAAGCAATGGCGTCTGTGTGATTAAGGCCATGAAAAGCTCGTCTCCCCCACGACTGACCTTGTCGAGCAATTGGGATTTTCCCTCAGCTACTACTTCAGCTCCAAGGCTGCCCAGATTCGAAGAGCTCGATACCACCAACATGCTTCTCCGTCAAAGAATCATCTTTCTGGGTTCTCAGGTAGATGACATGACAGCGGATTTGATCATAAGCCAACTTCTATTTCTAGATGCCGAGGACCCGAAAAAGGACATTAAATTGTTTATCAATTCGCCCGGTGGTTCTGTTACTGCTGGAATGGGAATATATGATGCAATGAAATTGTGCAAGGCAGATGTTACAACTGTTTGCCTGGGGCTTGCTGCTTCTATGGGGGCCTTTCTTCTTGCTGCTGGCACAAAGGGAAAGAGATTCTGCATGCCCAATGCAAGAGTGATGATCCATCAACCCCTTGGAACTGCCGGGGGAAAAGCAACAGAAATGGGTATTCGGATTAGAGAAATGGTGTACCACAAGATTAAATTGAACAAAATATTCTCAAGAATCACGGGGAAGCCTTTGGAGCAGATTGAAGAGGACACGGACCGTGATAATTTCATGAATCCTTGGGAAGCTAGGGATTATGGGTTGATTGATGTCGTTATCGATGATGGAAAGCCAGGCTTAGTTGCACCCGTTGGAGATTTCACACCTCCACCCAGAACACGAGTCTGGGATCAGTGGAAAGTTGAAGGGAGCAAGAAATCGAGAAAGAGCTTGCCCGCAGAACAAAGGATTTCTGACACTGAAAGAGGGGCCGATAGGTTAAAGGAAGCACCCACTGCTATATGA

Protein Analysis

317

Amino Acids

34.66

Weight (kDa)

9.19

Isoelectric Point (pI)

33.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLP_protease PF00574 83 - 256 1.9e-75 Clp protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 32, 317, 882
AclWI GGATC 2 cut(s) 553, 843
AcuI CTGAAG 1 cut(s) 201
AcyI GRCGYC 1 cut(s) 132
AfaI GTAC 2 cut(s) 11, 629
AfiI CCNNNNNNNGG 1 cut(s) 367
AgsI TTSAA 4 cut(s) 27, 646, 692, 851
AjnI CCWGG 2 cut(s) 471, 778
AjuI GAANNNNNNNTTGG 2 cut(s) 327, 359
AluBI AGCT 7 cut(s) 23, 159, 209, 221, 247, 734, 876
AluI AGCT 7 cut(s) 23, 159, 209, 221, 247, 734, 876
Alw21I GWGCWC 1 cut(s) 249
Alw26I GTCTC 1 cut(s) 168
AlwI GGATC 2 cut(s) 553, 843
AoxI GGCC 4 cut(s) 29, 147, 495, 915
ApeKI GCWGC 4 cut(s) 14, 229, 482, 509
ArsI GACNNNNNNTTYG 2 cut(s) 304, 336
AspS9I GGNCC 4 cut(s) 358, 495, 703, 915
AsuC2I CCSGG 2 cut(s) 396, 583
AsuII TTCGAA 1 cut(s) 240
AvaII GGWCC 2 cut(s) 358, 703
BaeGI GKGCMC 2 cut(s) 60, 83
BanII GRGCYC 1 cut(s) 249
BauI CACGAG 1 cut(s) 825
Bbv12I GWGCWC 1 cut(s) 249
BbvCI CCTCAGC 1 cut(s) 205
BbvI GCAGC 4 cut(s) 26, 216, 469, 496
BccI CCATC 2 cut(s) 570, 764
BciT130I CCWGG 2 cut(s) 473, 780
BclI TGATCA 1 cut(s) 324
BcnI CCSGG 2 cut(s) 396, 583
BcoDI GTCTC 1 cut(s) 168
BfaI CTAG 2 cut(s) 347, 735
BisI GCNGC 5 cut(s) 15, 32, 230, 483, 510
BlsI GCNGC 5 cut(s) 16, 33, 231, 484, 511
Bme1390I CCNGG 4 cut(s) 396, 473, 583, 780
Bme18I GGWCC 2 cut(s) 358, 703
BmgT120I GGNCC 4 cut(s) 358, 495, 703, 915
BmiI GGNNCC 3 cut(s) 360, 496, 916
BmrFI CCNGG 4 cut(s) 396, 473, 583, 780
BmsI GCATC 2 cut(s) 340, 421
Bpu10I CCTNAGC 1 cut(s) 205
Bpu14I TTCGAA 1 cut(s) 240
BpuEI CTTGAG 1 cut(s) 643
BpuMI CCSGG 2 cut(s) 396, 583
Bsa29I ATCGAT 1 cut(s) 765
BsaHI GRCGYC 1 cut(s) 132
BsaJI CCNNGG 6 cut(s) 224, 354, 472, 571, 582, 725
BsaXI ACNNNNNCTCC 2 cut(s) 205, 235
Bsc4I CCNNNNNNNGG 1 cut(s) 367
Bse3DI GCAATG 3 cut(s) 103, 133, 441
BseBI CCWGG 2 cut(s) 473, 780
BseCI ATCGAT 1 cut(s) 765
BseDI CCNNGG 6 cut(s) 224, 354, 472, 571, 582, 725
BseLI CCNNNNNNNGG 1 cut(s) 367
BseMI GCAATG 3 cut(s) 103, 133, 441
BseMII CTCAG 2 cut(s) 219, 311
BseSI GKGCMC 2 cut(s) 60, 83
BseXI GCAGC 4 cut(s) 26, 216, 469, 496
BshFI GGCC 4 cut(s) 31, 149, 497, 917
BshVI ATCGAT 1 cut(s) 765
BsiHKAI GWGCWC 1 cut(s) 249
BsiSI CCGG 2 cut(s) 396, 582
BsiWI CGTACG 1 cut(s) 9
BslI CCNNNNNNNGG 1 cut(s) 367
BsmAI GTCTC 1 cut(s) 168
BsmBI CGTCTC 1 cut(s) 168
BsnI GGCC 4 cut(s) 31, 149, 497, 917
Bsp119I TTCGAA 1 cut(s) 240
Bsp1286I GDGCHC 3 cut(s) 60, 83, 249
Bsp143I GATC 3 cut(s) 324, 558, 835
BspACI CCGC 3 cut(s) 32, 317, 882
BspANI GGCC 4 cut(s) 31, 149, 497, 917
BspCNI CTCAG 2 cut(s) 218, 310
BspDI ATCGAT 1 cut(s) 765
BspHI TCATGA 1 cut(s) 717
BspLI GGNNCC 3 cut(s) 360, 496, 916
BspPI GGATC 2 cut(s) 553, 843
BspQI GCTCTTC 1 cut(s) 237
BspT104I TTCGAA 1 cut(s) 240
BsrDI GCAATG 3 cut(s) 103, 133, 441
BssECI CCNNGG 6 cut(s) 224, 354, 472, 571, 582, 725
BssMI GATC 3 cut(s) 324, 558, 835
BssNI GRCGYC 1 cut(s) 132
BssSI CACGAG 1 cut(s) 825
BssT1I CCWWGG 3 cut(s) 224, 571, 725
Bst2BI CACGAG 1 cut(s) 825
Bst2UI CCWGG 2 cut(s) 473, 780
Bst4CI ACNGT 2 cut(s) 466, 707
Bst6I CTCTTC 2 cut(s) 237, 687
BstACI GRCGYC 1 cut(s) 132
BstAPI GCANNNNNTGC 1 cut(s) 941
BstBI TTCGAA 1 cut(s) 240
BstC8I GCNNGC 5 cut(s) 480, 514, 539, 878, 882
BstDEI CTNAG 3 cut(s) 205, 297, 784
BstKTI GATC 3 cut(s) 327, 561, 838
BstMAI GTCTC 1 cut(s) 168
BstMBI GATC 3 cut(s) 324, 558, 835
BstMWI GCNNNNNNNGC 4 cut(s) 20, 64, 682, 941
BstNI CCWGG 2 cut(s) 473, 780
BstNSI RCATGY 2 cut(s) 265, 541
BstSCI CCNGG 4 cut(s) 394, 471, 581, 778
BstSLI GKGCMC 2 cut(s) 60, 83
BstV1I GCAGC 4 cut(s) 26, 216, 469, 496
Bsu15I ATCGAT 1 cut(s) 765
BsuRI GGCC 4 cut(s) 31, 149, 497, 917
BsuTUI ATCGAT 1 cut(s) 765
BtsI GCAGTG 2 cut(s) 81, 939
BtsIMutI CAGTG 4 cut(s) 81, 845, 903, 939
Cac8I GCNNGC 5 cut(s) 480, 514, 539, 878, 882
CciI TCATGA 1 cut(s) 717
Cfr13I GGNCC 4 cut(s) 358, 495, 703, 915
ClaI ATCGAT 1 cut(s) 765
CpoI CGGWCCG 1 cut(s) 703
CseI GACGC 1 cut(s) 121
Csp6I GTAC 2 cut(s) 10, 628
CspI CGGWCCG 1 cut(s) 703
CviAII CATG 5 cut(s) 151, 262, 310, 538, 718
CviQI GTAC 2 cut(s) 10, 628
DdeI CTNAG 3 cut(s) 205, 297, 784
DpnI GATC 3 cut(s) 326, 560, 837
DpnII GATC 3 cut(s) 324, 558, 835
Eam1104I CTCTTC 2 cut(s) 237, 687
EarI CTCTTC 2 cut(s) 237, 687
Ecl136II GAGCTC 1 cut(s) 247
Eco130I CCWWGG 3 cut(s) 224, 571, 725
Eco24I GRGCYC 1 cut(s) 249
Eco47I GGWCC 2 cut(s) 358, 703
Eco53kI GAGCTC 1 cut(s) 247
Eco57I CTGAAG 1 cut(s) 201
EcoICRI GAGCTC 1 cut(s) 247
EcoO109I RGGNCCY 2 cut(s) 358, 495
EcoRII CCWGG 2 cut(s) 471, 778
EcoT14I CCWWGG 3 cut(s) 224, 571, 725
EcoT38I GRGCYC 1 cut(s) 249
ErhI CCWWGG 3 cut(s) 224, 571, 725
Esp3I CGTCTC 1 cut(s) 168
FaeI CATG 5 cut(s) 154, 265, 313, 541, 721
FalI AAGNNNNNCTT 2 cut(s) 767, 799
FatI CATG 5 cut(s) 150, 261, 309, 537, 717
FauI CCCGC 1 cut(s) 889
FbaI TGATCA 1 cut(s) 324
Fnu4HI GCNGC 5 cut(s) 15, 32, 230, 483, 510
FriOI GRGCYC 1 cut(s) 249
Fsp4HI GCNGC 5 cut(s) 15, 32, 230, 483, 510
FspBI CTAG 2 cut(s) 347, 735
GluI GCNGC 5 cut(s) 15, 32, 230, 483, 510
HaeIII GGCC 4 cut(s) 31, 149, 497, 917
HapII CCGG 2 cut(s) 396, 582
HgaI GACGC 1 cut(s) 121
Hin1I GRCGYC 1 cut(s) 132
Hin1II CATG 5 cut(s) 154, 265, 313, 541, 721
HinfI GANTC 6 cut(s) 237, 279, 531, 663, 721, 828
HpaII CCGG 2 cut(s) 396, 582
Hpy166II GTNNAC 1 cut(s) 628
Hpy188I TCNGA 2 cut(s) 612, 901
Hpy188III TCNNGA 4 cut(s) 347, 660, 718, 867
Hpy8I GTNNAC 1 cut(s) 628
HpyAV CCTTC 2 cut(s) 70, 845
HpyCH4III ACNGT 2 cut(s) 466, 707
HpyCH4IV ACGT 1 cut(s) 8
HpyCH4V TGCA 5 cut(s) 434, 447, 537, 548, 791
HpyF10VI GCNNNNNNNGC 4 cut(s) 20, 64, 682, 941
HpyF3I CTNAG 3 cut(s) 205, 297, 784
HpySE526I ACGT 1 cut(s) 8
Hsp92I GRCGYC 1 cut(s) 132
Hsp92II CATG 5 cut(s) 154, 265, 313, 541, 721
Ksp22I TGATCA 1 cut(s) 324
Kzo9I GATC 3 cut(s) 324, 558, 835
LguI GCTCTTC 1 cut(s) 237
LmnI GCTCC 3 cut(s) 226, 682, 855
Lsp1109I GCAGC 4 cut(s) 26, 216, 469, 496
LweI GCATC 2 cut(s) 340, 421
MaeI CTAG 2 cut(s) 347, 735
MaeII ACGT 1 cut(s) 8
MaeIII GTNAC 2 cut(s) 406, 457
MalI GATC 3 cut(s) 326, 560, 837
MboI GATC 3 cut(s) 324, 558, 835
MboII GAAGA 4 cut(s) 33, 254, 494, 704
MfeI CAATTG 3 cut(s) 69, 109, 190
MhlI GDGCHC 3 cut(s) 60, 83, 249
MluCI AATT 8 cut(s) 69, 109, 190, 377, 388, 440, 641, 712
MlyI GAGTC 1 cut(s) 837
MmeI TCCRAC 1 cut(s) 778
MnlI CCTC 6 cut(s) 98, 214, 349, 688, 822, 905
MseI TTAA 4 cut(s) 144, 375, 639, 926
MslI CAYNNNNRTG 1 cut(s) 260
MspA1I CMGCKG 2 cut(s) 34, 317
MspI CCGG 2 cut(s) 396, 582
MspR9I CCNGG 4 cut(s) 396, 473, 583, 780
MunI CAATTG 3 cut(s) 69, 109, 190
MvaI CCWGG 2 cut(s) 473, 780
MwoI GCNNNNNNNGC 4 cut(s) 20, 64, 682, 941
NciI CCSGG 2 cut(s) 396, 583
NdeII GATC 3 cut(s) 324, 558, 835
NlaIII CATG 5 cut(s) 154, 265, 313, 541, 721
NlaIV GGNNCC 3 cut(s) 360, 496, 916
NmeAIII GCCGAG 1 cut(s) 379
NspI RCATGY 2 cut(s) 265, 541
NspV TTCGAA 1 cut(s) 240
PaeI GCATGC 1 cut(s) 541
PagI TCATGA 1 cut(s) 717
PciSI GCTCTTC 1 cut(s) 237
PcsI WCGNNNNNNNCGW 1 cut(s) 246
PfeI GAWTC 5 cut(s) 237, 279, 531, 663, 721
Pfl23II CGTACG 1 cut(s) 9
PflFI GACNNNGTC 1 cut(s) 181
PkrI GCNGC 5 cut(s) 16, 33, 231, 484, 511
PleI GAGTC 1 cut(s) 836
PpsI GAGTC 1 cut(s) 836
PpuMI RGGWCCY 1 cut(s) 358
Psp124BI GAGCTC 1 cut(s) 249
Psp5II RGGWCCY 1 cut(s) 358
Psp6I CCWGG 2 cut(s) 471, 778
PspGI CCWGG 2 cut(s) 471, 778
PspLI CGTACG 1 cut(s) 9
PspN4I GGNNCC 3 cut(s) 360, 496, 916
PspPI GGNCC 4 cut(s) 358, 495, 703, 915
PspPPI RGGWCCY 1 cut(s) 358
PsyI GACNNNGTC 1 cut(s) 181
RsaI GTAC 2 cut(s) 11, 629
RsaNI GTAC 2 cut(s) 10, 628
RseI CAYNNNNRTG 1 cut(s) 260
Rsr2I CGGWCCG 1 cut(s) 703
RsrII CGGWCCG 1 cut(s) 703
SacI GAGCTC 1 cut(s) 249
SapI GCTCTTC 1 cut(s) 237
SaqAI TTAA 4 cut(s) 144, 375, 639, 926
SatI GCNGC 5 cut(s) 15, 32, 230, 483, 510
Sau3AI GATC 3 cut(s) 324, 558, 835
Sau96I GGNCC 4 cut(s) 358, 495, 703, 915
SchI GAGTC 1 cut(s) 837
ScrFI CCNGG 4 cut(s) 396, 473, 583, 780
SduI GDGCHC 3 cut(s) 60, 83, 249
SfaNI GCATC 2 cut(s) 340, 421
SfuI TTCGAA 1 cut(s) 240
SinI GGWCC 2 cut(s) 358, 703
SmiMI CAYNNNNRTG 1 cut(s) 260
SmlI CTYRAG 1 cut(s) 658
SmoI CTYRAG 1 cut(s) 658
SphI GCATGC 1 cut(s) 541
Sse9I AATT 8 cut(s) 69, 109, 190, 377, 388, 440, 641, 712
SsiI CCGC 3 cut(s) 32, 317, 882
SspI AATATT 1 cut(s) 654
SspMI CTAG 2 cut(s) 347, 735
SstI GAGCTC 1 cut(s) 249
StyD4I CCNGG 4 cut(s) 394, 471, 581, 778
StyI CCWWGG 3 cut(s) 224, 571, 725
TaaI ACNGT 2 cut(s) 466, 707
TaiI ACGT 1 cut(s) 11
TaqI TCGA 5 cut(s) 185, 240, 249, 765, 866
TasI AATT 8 cut(s) 69, 109, 190, 377, 388, 440, 641, 712
TauI GCSGC 1 cut(s) 34
TfiI GAWTC 5 cut(s) 237, 279, 531, 663, 721
Tru1I TTAA 4 cut(s) 144, 375, 639, 926
Tru9I TTAA 4 cut(s) 144, 375, 639, 926
TscAI CASTG 4 cut(s) 88, 845, 910, 946
TseI GCWGC 4 cut(s) 14, 229, 482, 509
TspDTI ATGAA 4 cut(s) 167, 452, 706, 734
TspGWI ACGGA 2 cut(s) 260, 716
TspRI CASTG 4 cut(s) 88, 845, 910, 946
Tth111I GACNNNGTC 1 cut(s) 181
VpaK11BI GGWCC 2 cut(s) 358, 703
XbaI TCTAGA 1 cut(s) 346
XceI RCATGY 2 cut(s) 265, 541
XcmI CCANNNNNNNNNTGG 1 cut(s) 569
XspI CTAG 2 cut(s) 347, 735
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.