Rw1G006960

ATP-dependent Clp protease proteolytic subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0003024
Physical Location & Seq
Reverse (-)
14459131 .. 14464643
5513 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G006960.1

Sequence Viewer

Length: 939 bp
ATGATGACATACGCAGCCTCAGCTTCAAGGCCTCTCTGTTTCAACACCCAACCGGCCATTGTGAACACGGTCTTAATAGCAGTAGCGCTTCTACGCGTCGGTCTAGTTCAAGCCTCGTCGCCTACAAGACCCACCTTGTCCAGCAATTGGGATTTTCAGTTCGCAGCTACTACTACTACTTCCTCTCCACGCGTCCCCAGATTCGAAGAGCTCGACACCACCAACATGCTCCTCCGTCAGCGAATCATCTTCTTGGGTTCTCAGGTGGATGATATGACGGCGGATTTGATAATAAGCCAACTGCTATTTCTGGATGCTGAAGACGAAGACCCCAAAAAGGACATTACATTGTTTATCAATTCGCCTGGTGGCTCGGTTACTGCTGGAATGGGAATATATGATGCAATGAAATTGTGCAAGGCAGATGTTTCAACTGTTTGCTTGGGGCTTGCTGCTTCTATGGGTGCATTTCTCCTTGCTTCCGGTTCAAAAGGAAAGAGGTACTGCATGCCCAATGCAAGAGTGATGATCCATCAACCTCTTGGAACTGCTGGAGGCAAAGCAACAGAAATGGGTATACGAATTAGAGAAATGGCGTACCACAAGATTAAATTGAACAAAATATTGTCAAGAATCACAGGGAAGCCTTTAGACCAGGTTGAGGAGGACACAGACCGTGATAATTTCATGAATCCCTGGGAAGCTAGGGATTATGGGTTGATTGATGAGGTTATTGATGATGGAAAGCCAGGGTTAGTTGCACCAATTGGAGATTCCACACCTCCACCCAAAACAAAAGTCTGGGATATGTGGAAAGTTGAAGGTACCAAGAAATCCAGAAAGAATTTACCCTCGGAGCTGAAAATTTCAGATAATGGATCTAAGGGGAGTGAAGGTCGTGAGAATGAGAAAGGTTTGGAAGCACCTACTGCAACATAG

Protein Analysis

312

Amino Acids

33.99

Weight (kDa)

6.22

Isoelectric Point (pI)

30.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLP_protease PF00574 71 - 246 3.7e-73 Clp protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 824
AccB1I GGYRCC 1 cut(s) 824
AccB7I CCANNNNNTGG 1 cut(s) 147
AccI GTMKAC 1 cut(s) 577
AccII CGCG 2 cut(s) 96, 192
AciI CCGC 1 cut(s) 281
AclWI GGATC 2 cut(s) 523, 886
AcoI YGGCCR 1 cut(s) 54
AcsI RAATTY 2 cut(s) 844, 864
AcuI CTGAAG 1 cut(s) 339
AfaI GTAC 3 cut(s) 503, 599, 826
AfeI AGCGCT 1 cut(s) 87
AfiI CCNNNNNNNGG 3 cut(s) 147, 337, 661
AflIII ACRYGT 2 cut(s) 94, 190
AgsI TTSAA 7 cut(s) 27, 43, 110, 432, 489, 616, 821
AjnI CCWGG 4 cut(s) 364, 654, 695, 748
AjuI GAANNNNNNNTTGG 4 cut(s) 291, 323, 757, 789
AloI GAACNNNNNNTCC 2 cut(s) 143, 175
AluBI AGCT 5 cut(s) 23, 167, 211, 704, 859
AluI AGCT 5 cut(s) 23, 167, 211, 704, 859
Alw21I GWGCWC 1 cut(s) 213
AlwI GGATC 2 cut(s) 523, 886
Aor51HI AGCGCT 1 cut(s) 87
AoxI GGCC 2 cut(s) 29, 54
ApeKI GCWGC 3 cut(s) 14, 164, 452
ApoI RAATTY 2 cut(s) 844, 864
ArsI GACNNNNNNTTYG 4 cut(s) 268, 300, 783, 815
Asp718I GGTACC 1 cut(s) 824
AspLEI GCGC 1 cut(s) 88
AsuII TTCGAA 1 cut(s) 204
BanI GGYRCC 1 cut(s) 824
BanII GRGCYC 1 cut(s) 213
BbsI GAAGAC 2 cut(s) 327, 333
Bbv12I GWGCWC 1 cut(s) 213
BbvCI CCTCAGC 1 cut(s) 19
BbvI GCAGC 3 cut(s) 26, 176, 439
BccI CCATC 2 cut(s) 540, 734
BceAI ACGGC 1 cut(s) 294
BciT130I CCWGG 4 cut(s) 366, 656, 697, 750
BfaI CTAG 2 cut(s) 104, 705
BfoI RGCGCY 1 cut(s) 89
BisI GCNGC 3 cut(s) 15, 165, 453
BlsI GCNGC 3 cut(s) 16, 166, 454
Bme1390I CCNGG 4 cut(s) 366, 656, 697, 750
BmiI GGNNCC 1 cut(s) 826
BmrFI CCNGG 4 cut(s) 366, 656, 697, 750
BmsI GCATC 2 cut(s) 304, 391
BpiI GAAGAC 2 cut(s) 327, 333
BpmI CTGGAG 1 cut(s) 573
Bpu10I CCTNAGC 1 cut(s) 19
Bpu14I TTCGAA 1 cut(s) 204
BsaJI CCNNGG 4 cut(s) 695, 696, 749, 852
BsaWI WCCGGW 1 cut(s) 482
BsaXI ACNNNNNCTCC 4 cut(s) 169, 199, 880, 910
Bsc4I CCNNNNNNNGG 3 cut(s) 147, 337, 661
Bse118I RCCGGY 1 cut(s) 52
Bse3DI GCAATG 1 cut(s) 411
BseBI CCWGG 4 cut(s) 366, 656, 697, 750
BseDI CCNNGG 4 cut(s) 695, 696, 749, 852
BseGI GGATG 2 cut(s) 274, 319
BseLI CCNNNNNNNGG 3 cut(s) 147, 337, 661
BseMI GCAATG 1 cut(s) 411
BseMII CTCAG 2 cut(s) 33, 275
BseRI GAGGAG 2 cut(s) 221, 677
BseXI GCAGC 3 cut(s) 26, 176, 439
Bsh1236I CGCG 2 cut(s) 96, 192
BshFI GGCC 2 cut(s) 31, 56
BshNI GGYRCC 1 cut(s) 824
BsiHKAI GWGCWC 1 cut(s) 213
BsiSI CCGG 2 cut(s) 53, 483
BslFI GGGAC 1 cut(s) 179
BslI CCNNNNNNNGG 3 cut(s) 147, 337, 661
BsmFI GGGAC 1 cut(s) 179
BsnI GGCC 2 cut(s) 31, 56
Bsp119I TTCGAA 1 cut(s) 204
Bsp1286I GDGCHC 1 cut(s) 213
Bsp143I GATC 2 cut(s) 528, 878
BspACI CCGC 1 cut(s) 281
BspANI GGCC 2 cut(s) 31, 56
BspCNI CTCAG 2 cut(s) 32, 274
BspFNI CGCG 2 cut(s) 96, 192
BspHI TCATGA 1 cut(s) 687
BspLI GGNNCC 1 cut(s) 826
BspPI GGATC 2 cut(s) 523, 886
BspQI GCTCTTC 1 cut(s) 201
BspT104I TTCGAA 1 cut(s) 204
BspT107I GGYRCC 1 cut(s) 824
BsrDI GCAATG 1 cut(s) 411
BsrFI RCCGGY 1 cut(s) 52
BssAI RCCGGY 1 cut(s) 52
BssECI CCNNGG 4 cut(s) 695, 696, 749, 852
BssMI GATC 2 cut(s) 528, 878
BssNAI GTATAC 1 cut(s) 578
Bst1107I GTATAC 1 cut(s) 578
Bst2UI CCWGG 4 cut(s) 366, 656, 697, 750
Bst4CI ACNGT 3 cut(s) 70, 436, 677
Bst6I CTCTTC 1 cut(s) 201
BstAPI GCANNNNNTGC 1 cut(s) 929
BstBI TTCGAA 1 cut(s) 204
BstC8I GCNNGC 2 cut(s) 450, 509
BstDEI CTNAG 3 cut(s) 19, 261, 882
BstF5I GGATG 2 cut(s) 274, 319
BstFNI CGCG 2 cut(s) 96, 192
BstH2I RGCGCY 1 cut(s) 89
BstHHI GCGC 1 cut(s) 88
BstKTI GATC 2 cut(s) 531, 881
BstMBI GATC 2 cut(s) 528, 878
BstMWI GCNNNNNNNGC 2 cut(s) 20, 929
BstNI CCWGG 4 cut(s) 366, 656, 697, 750
BstNSI RCATGY 2 cut(s) 229, 511
BstSCI CCNGG 4 cut(s) 364, 654, 695, 748
BstUI CGCG 2 cut(s) 96, 192
BstV1I GCAGC 3 cut(s) 26, 176, 439
BstV2I GAAGAC 2 cut(s) 327, 333
BstX2I RGATCY 1 cut(s) 878
BstYI RGATCY 1 cut(s) 878
BstZ17I GTATAC 1 cut(s) 578
BsuRI GGCC 2 cut(s) 31, 56
BtsCI GGATG 2 cut(s) 274, 319
Cac8I GCNNGC 2 cut(s) 450, 509
CciI TCATGA 1 cut(s) 687
CfoI GCGC 1 cut(s) 88
Cfr10I RCCGGY 1 cut(s) 52
CseI GACGC 2 cut(s) 85, 181
CsiI ACCWGGT 1 cut(s) 654
Csp6I GTAC 3 cut(s) 502, 598, 825
CviAII CATG 3 cut(s) 226, 508, 688
CviQI GTAC 3 cut(s) 502, 598, 825
DdeI CTNAG 3 cut(s) 19, 261, 882
DpnI GATC 2 cut(s) 530, 880
DpnII GATC 2 cut(s) 528, 878
EaeI YGGCCR 1 cut(s) 54
Eam1104I CTCTTC 1 cut(s) 201
EarI CTCTTC 1 cut(s) 201
EciI GGCGGA 1 cut(s) 296
Ecl136II GAGCTC 1 cut(s) 211
Eco147I AGGCCT 1 cut(s) 31
Eco24I GRGCYC 1 cut(s) 213
Eco47III AGCGCT 1 cut(s) 87
Eco53kI GAGCTC 1 cut(s) 211
Eco57I CTGAAG 1 cut(s) 339
EcoICRI GAGCTC 1 cut(s) 211
EcoRII CCWGG 4 cut(s) 364, 654, 695, 748
EcoT38I GRGCYC 1 cut(s) 213
FaeI CATG 3 cut(s) 229, 511, 691
FaqI GGGAC 1 cut(s) 179
FatI CATG 3 cut(s) 225, 507, 687
FblI GTMKAC 1 cut(s) 577
Fnu4HI GCNGC 3 cut(s) 15, 165, 453
FokI GGATG 2 cut(s) 281, 326
FriOI GRGCYC 1 cut(s) 213
Fsp4HI GCNGC 3 cut(s) 15, 165, 453
FspBI CTAG 2 cut(s) 104, 705
GlaI GCGC 1 cut(s) 87
GluI GCNGC 3 cut(s) 15, 165, 453
GsuI CTGGAG 1 cut(s) 573
HaeII RGCGCY 1 cut(s) 89
HaeIII GGCC 2 cut(s) 31, 56
HapII CCGG 2 cut(s) 53, 483
HgaI GACGC 2 cut(s) 85, 181
HhaI GCGC 1 cut(s) 88
Hin1II CATG 3 cut(s) 229, 511, 691
Hin6I GCGC 1 cut(s) 86
HinP1I GCGC 1 cut(s) 86
HinfI GANTC 5 cut(s) 201, 243, 633, 691, 773
HpaII CCGG 2 cut(s) 53, 483
Hpy166II GTNNAC 2 cut(s) 64, 578
Hpy188I TCNGA 2 cut(s) 856, 871
Hpy188III TCNNGA 5 cut(s) 311, 630, 688, 837, 899
Hpy8I GTNNAC 2 cut(s) 64, 578
Hpy99I CGWCG 2 cut(s) 101, 121
HpyAV CCTTC 2 cut(s) 815, 887
HpyCH4III ACNGT 3 cut(s) 70, 436, 677
HpyCH4V TGCA 7 cut(s) 404, 417, 467, 507, 518, 761, 932
HpyF10VI GCNNNNNNNGC 2 cut(s) 20, 929
HpyF3I CTNAG 3 cut(s) 19, 261, 882
Hsp92II CATG 3 cut(s) 229, 511, 691
HspAI GCGC 1 cut(s) 86
KpnI GGTACC 1 cut(s) 828
Kzo9I GATC 2 cut(s) 528, 878
LguI GCTCTTC 1 cut(s) 201
LmnI GCTCC 2 cut(s) 234, 856
Lsp1109I GCAGC 3 cut(s) 26, 176, 439
LweI GCATC 2 cut(s) 304, 391
MabI ACCWGGT 1 cut(s) 654
MaeI CTAG 2 cut(s) 104, 705
MaeIII GTNAC 1 cut(s) 376
MalI GATC 2 cut(s) 530, 880
MboI GATC 2 cut(s) 528, 878
MboII GAAGA 4 cut(s) 218, 241, 332, 338
MfeI CAATTG 2 cut(s) 145, 765
MflI RGATCY 1 cut(s) 878
MhlI GDGCHC 1 cut(s) 213
MluCI AATT 9 cut(s) 145, 358, 410, 582, 611, 682, 765, 844, 864
MluI ACGCGT 2 cut(s) 94, 190
MseI TTAA 2 cut(s) 74, 609
MslI CAYNNNNRTG 1 cut(s) 224
MspI CCGG 2 cut(s) 53, 483
MspR9I CCNGG 4 cut(s) 366, 656, 697, 750
MunI CAATTG 2 cut(s) 145, 765
MvaI CCWGG 4 cut(s) 366, 656, 697, 750
MvnI CGCG 2 cut(s) 96, 192
MwoI GCNNNNNNNGC 2 cut(s) 20, 929
NdeII GATC 2 cut(s) 528, 878
NlaIII CATG 3 cut(s) 229, 511, 691
NlaIV GGNNCC 1 cut(s) 826
NspI RCATGY 2 cut(s) 229, 511
NspV TTCGAA 1 cut(s) 204
PaeI GCATGC 1 cut(s) 511
PagI TCATGA 1 cut(s) 687
PasI CCCWGGG 1 cut(s) 696
PceI AGGCCT 1 cut(s) 31
PciSI GCTCTTC 1 cut(s) 201
PcsI WCGNNNNNNNCGW 1 cut(s) 210
PfeI GAWTC 5 cut(s) 201, 243, 633, 691, 773
PflMI CCANNNNNTGG 1 cut(s) 147
PkrI GCNGC 3 cut(s) 16, 166, 454
Psp124BI GAGCTC 1 cut(s) 213
Psp6I CCWGG 4 cut(s) 364, 654, 695, 748
PspGI CCWGG 4 cut(s) 364, 654, 695, 748
PspN4I GGNNCC 1 cut(s) 826
PsuI RGATCY 1 cut(s) 878
RsaI GTAC 3 cut(s) 503, 599, 826
RsaNI GTAC 3 cut(s) 502, 598, 825
RseI CAYNNNNRTG 1 cut(s) 224
SacI GAGCTC 1 cut(s) 213
SapI GCTCTTC 1 cut(s) 201
SaqAI TTAA 2 cut(s) 74, 609
SatI GCNGC 3 cut(s) 15, 165, 453
Sau3AI GATC 2 cut(s) 528, 878
ScrFI CCNGG 4 cut(s) 366, 656, 697, 750
SduI GDGCHC 1 cut(s) 213
SexAI ACCWGGT 1 cut(s) 654
SfaNI GCATC 2 cut(s) 304, 391
SfuI TTCGAA 1 cut(s) 204
SmiMI CAYNNNNRTG 1 cut(s) 224
SphI GCATGC 1 cut(s) 511
Sse9I AATT 9 cut(s) 145, 358, 410, 582, 611, 682, 765, 844, 864
SseBI AGGCCT 1 cut(s) 31
SsiI CCGC 1 cut(s) 281
SspI AATATT 1 cut(s) 624
SspMI CTAG 2 cut(s) 104, 705
SstI GAGCTC 1 cut(s) 213
StuI AGGCCT 1 cut(s) 31
StyD4I CCNGG 4 cut(s) 364, 654, 695, 748
TaaI ACNGT 3 cut(s) 70, 436, 677
TaqI TCGA 2 cut(s) 204, 213
TaqII GACCGA 1 cut(s) 89
TasI AATT 9 cut(s) 145, 358, 410, 582, 611, 682, 765, 844, 864
TfiI GAWTC 5 cut(s) 201, 243, 633, 691, 773
Tru1I TTAA 2 cut(s) 74, 609
Tru9I TTAA 2 cut(s) 74, 609
TseI GCWGC 3 cut(s) 14, 164, 452
TspDTI ATGAA 3 cut(s) 422, 676, 704
TspGWI ACGGA 1 cut(s) 224
Van91I CCANNNNNTGG 1 cut(s) 147
XapI RAATTY 2 cut(s) 844, 864
XceI RCATGY 2 cut(s) 229, 511
XcmI CCANNNNNNNNNTGG 1 cut(s) 539
XmiI GTMKAC 1 cut(s) 577
XspI CTAG 2 cut(s) 104, 705
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.