AT1G74440

Protein of unknown function (DUF962)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0002194
Physical Location & Seq
Reverse (-)
27976502 .. 27980456
3955 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G74440.2

Sequence Viewer

Length: 627 bp
ATGAGCAATCGCATCGGATTGCTCGATCTAGAGAAGCATTTTGCTTTCTATGGAGCTTATCACAGCAATCCCATTAACATTATAATCCACACTCTCTTCGTATGGCCAAACGTCTTCGCTACTCTCCTTTTCCTCTACTCTACGCCACCGATTTTAGATCACTCTCAATTAGGGTTCCTCAAGTCGCTGACATTTGATGGTGTTTTGCGTTTGGACATTGGTTTTACCCTCACTGTGACTTATGCTGTCTTTTACATCTGTTTGGATAAGAAATCTGGTGTTTTAGCAGCTCTGTTATGTTTCTCTTGTTGGATTGGCTCTAGCTTCCTCGCAGCTCGATTAGGACATTCCTTGACTTTAAAGGTTGGAGTAGCTTCTCAGCTCTTATGTTGGACTGGTCAGTTCCTCGGACATGGCTTGTTTGAGAAACGAGCTCCTGCACTTTTAGACAATCTACTCCAAGCTTTTCTTATGGGTCCTTTCTTTGTGTTGCTCGAGGTGTTGCAGTCTGTTTTCGGGTACGAGCCATACCCTGGATTTAAAGCACGCGTCGATTCCAAGATAGAGAGTGGAATCAAGGAATGGAGAGAAAAGAAGAAACAGCAGCAGATGAAGAAGGTCACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF962

Protein Analysis

208

Amino Acids

23.44

Weight (kDa)

9.16

Isoelectric Point (pI)

37.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mpo1-like PF06127 9 - 170 8e-23 2-hydroxy-palmitic acid dioxygenase Mpo1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 83
AccB7I CCANNNNNTGG 1 cut(s) 533
AccII CGCG 1 cut(s) 549
AcoI YGGCCR 1 cut(s) 104
AfaI GTAC 1 cut(s) 521
AfiI CCNNNNNNNGG 1 cut(s) 533
AflIII ACRYGT 1 cut(s) 547
AjnI CCWGG 1 cut(s) 532
AluBI AGCT 8 cut(s) 56, 290, 324, 335, 374, 382, 434, 464
AluI AGCT 8 cut(s) 56, 290, 324, 335, 374, 382, 434, 464
Alw21I GWGCWC 1 cut(s) 436
Ama87I CYCGRG 1 cut(s) 494
AoxI GGCC 1 cut(s) 104
ApeKI GCWGC 3 cut(s) 287, 332, 604
AspS9I GGNCC 1 cut(s) 476
AvaI CYCGRG 1 cut(s) 494
AvaII GGWCC 1 cut(s) 476
BalI TGGCCA 1 cut(s) 106
BanII GRGCYC 1 cut(s) 436
BbsI GAAGAC 1 cut(s) 106
Bbv12I GWGCWC 1 cut(s) 436
BbvI GCAGC 3 cut(s) 299, 344, 616
BccI CCATC 1 cut(s) 191
BcgI CGANNNNNNTGC 1 cut(s) 29
BciT130I CCWGG 1 cut(s) 534
BfaI CTAG 2 cut(s) 29, 321
BisI GCNGC 3 cut(s) 288, 333, 605
BlsI GCNGC 3 cut(s) 289, 334, 606
Bme1390I CCNGG 1 cut(s) 534
Bme18I GGWCC 1 cut(s) 476
BmeT110I CYCGRG 1 cut(s) 494
BmgT120I GGNCC 1 cut(s) 476
BmiI GGNNCC 2 cut(s) 176, 477
BmrFI CCNGG 1 cut(s) 534
BmsI GCATC 1 cut(s) 21
BpiI GAAGAC 1 cut(s) 106
BpuEI CTTGAG 1 cut(s) 164
BsaJI CCNNGG 2 cut(s) 406, 532
Bsc4I CCNNNNNNNGG 1 cut(s) 533
Bse1I ACTGG 1 cut(s) 400
BseBI CCWGG 1 cut(s) 534
BseDI CCNNGG 2 cut(s) 406, 532
BseLI CCNNNNNNNGG 1 cut(s) 533
BseMII CTCAG 1 cut(s) 392
BseNI ACTGG 1 cut(s) 400
BseXI GCAGC 3 cut(s) 299, 344, 616
BsgI GTGCAG 1 cut(s) 423
Bsh1236I CGCG 1 cut(s) 549
BshFI GGCC 1 cut(s) 106
BsiHKAI GWGCWC 1 cut(s) 436
BsiHKCI CYCGRG 1 cut(s) 494
BslI CCNNNNNNNGG 1 cut(s) 533
BsnI GGCC 1 cut(s) 106
BsoBI CYCGRG 1 cut(s) 494
Bsp1286I GDGCHC 1 cut(s) 436
Bsp143I GATC 2 cut(s) 25, 157
BspANI GGCC 1 cut(s) 106
BspCNI CTCAG 1 cut(s) 391
BspFNI CGCG 1 cut(s) 549
BspLI GGNNCC 2 cut(s) 176, 477
BsrI ACTGG 1 cut(s) 400
BssECI CCNNGG 2 cut(s) 406, 532
BssMI GATC 2 cut(s) 25, 157
Bst2UI CCWGG 1 cut(s) 534
Bst4CI ACNGT 1 cut(s) 235
Bst6I CTCTTC 1 cut(s) 101
BstC8I GCNNGC 1 cut(s) 547
BstDEI CTNAG 1 cut(s) 378
BstFNI CGCG 1 cut(s) 549
BstKTI GATC 2 cut(s) 28, 160
BstMBI GATC 2 cut(s) 25, 157
BstNI CCWGG 1 cut(s) 534
BstSCI CCNGG 1 cut(s) 532
BstUI CGCG 1 cut(s) 549
BstV1I GCAGC 3 cut(s) 299, 344, 616
BstV2I GAAGAC 1 cut(s) 106
BsuRI GGCC 1 cut(s) 106
BtsIMutI CAGTG 1 cut(s) 231
Cac8I GCNNGC 1 cut(s) 547
Cfr13I GGNCC 1 cut(s) 476
CseI GACGC 1 cut(s) 538
Csp6I GTAC 1 cut(s) 520
CviAII CATG 1 cut(s) 413
CviQI GTAC 1 cut(s) 520
DdeI CTNAG 1 cut(s) 378
DpnI GATC 2 cut(s) 27, 159
DpnII GATC 2 cut(s) 25, 157
DraI TTTAAA 2 cut(s) 360, 541
EaeI YGGCCR 1 cut(s) 104
Eam1104I CTCTTC 1 cut(s) 101
EarI CTCTTC 1 cut(s) 101
Ecl136II GAGCTC 1 cut(s) 434
Eco24I GRGCYC 1 cut(s) 436
Eco47I GGWCC 1 cut(s) 476
Eco53kI GAGCTC 1 cut(s) 434
Eco88I CYCGRG 1 cut(s) 494
EcoICRI GAGCTC 1 cut(s) 434
EcoO109I RGGNCCY 1 cut(s) 476
EcoRII CCWGG 1 cut(s) 532
EcoT38I GRGCYC 1 cut(s) 436
FaeI CATG 1 cut(s) 416
FaiI YATR 9 cut(s) 51, 83, 103, 243, 298, 388, 414, 473, 529
FalI AAGNNNNNCTT 2 cut(s) 453, 485
FatI CATG 1 cut(s) 412
Fnu4HI GCNGC 3 cut(s) 288, 333, 605
FriOI GRGCYC 1 cut(s) 436
Fsp4HI GCNGC 3 cut(s) 288, 333, 605
FspBI CTAG 2 cut(s) 29, 321
GluI GCNGC 3 cut(s) 288, 333, 605
HaeIII GGCC 1 cut(s) 106
HgaI GACGC 1 cut(s) 538
Hin1II CATG 1 cut(s) 416
HindIII AAGCTT 1 cut(s) 462
HinfI GANTC 2 cut(s) 554, 573
Hpy188I TCNGA 2 cut(s) 17, 410
Hpy188III TCNNGA 1 cut(s) 29
Hpy99I CGWCG 1 cut(s) 554
HpyAV CCTTC 1 cut(s) 610
HpyCH4III ACNGT 1 cut(s) 235
HpyCH4IV ACGT 1 cut(s) 111
HpyCH4V TGCA 2 cut(s) 440, 505
HpyF3I CTNAG 1 cut(s) 378
HpySE526I ACGT 1 cut(s) 111
Hsp92II CATG 1 cut(s) 416
Kzo9I GATC 2 cut(s) 25, 157
LmnI GCTCC 2 cut(s) 53, 439
LpnPI CCDG 5 cut(s) 261, 381, 450, 519, 546
Lsp1109I GCAGC 3 cut(s) 299, 344, 616
LweI GCATC 1 cut(s) 21
MaeI CTAG 2 cut(s) 29, 321
MaeII ACGT 1 cut(s) 111
MaeIII GTNAC 2 cut(s) 235, 619
MalI GATC 2 cut(s) 27, 159
MboI GATC 2 cut(s) 25, 157
MboII GAAGA 4 cut(s) 88, 106, 607, 625
MhlI GDGCHC 1 cut(s) 436
MlsI TGGCCA 1 cut(s) 106
MluCI AATT 1 cut(s) 167
MluI ACGCGT 1 cut(s) 547
MluNI TGGCCA 1 cut(s) 106
MmeI TCCRAC 3 cut(s) 290, 346, 371
MnlI CCTC 6 cut(s) 143, 188, 239, 338, 416, 490
Mox20I TGGCCA 1 cut(s) 106
MscI TGGCCA 1 cut(s) 106
MseI TTAA 4 cut(s) 75, 359, 540, 625
Msp20I TGGCCA 1 cut(s) 106
MspR9I CCNGG 1 cut(s) 534
MvaI CCWGG 1 cut(s) 534
MvnI CGCG 1 cut(s) 549
NdeII GATC 2 cut(s) 25, 157
NlaIII CATG 1 cut(s) 416
NlaIV GGNNCC 2 cut(s) 176, 477
NmuCI GTSAC 2 cut(s) 235, 619
PaeR7I CTCGAG 1 cut(s) 494
PcsI WCGNNNNNNNCGW 1 cut(s) 21
PfeI GAWTC 2 cut(s) 554, 573
PflMI CCANNNNNTGG 1 cut(s) 533
PkrI GCNGC 3 cut(s) 289, 334, 606
PpuMI RGGWCCY 1 cut(s) 476
PsiI TTATAA 1 cut(s) 83
Psp124BI GAGCTC 1 cut(s) 436
Psp5II RGGWCCY 1 cut(s) 476
Psp6I CCWGG 1 cut(s) 532
PspGI CCWGG 1 cut(s) 532
PspN4I GGNNCC 2 cut(s) 176, 477
PspPI GGNCC 1 cut(s) 476
PspPPI RGGWCCY 1 cut(s) 476
PspXI VCTCGAGB 1 cut(s) 494
RsaI GTAC 1 cut(s) 521
RsaNI GTAC 1 cut(s) 520
SacI GAGCTC 1 cut(s) 436
SaqAI TTAA 4 cut(s) 75, 359, 540, 625
SatI GCNGC 3 cut(s) 288, 333, 605
Sau3AI GATC 2 cut(s) 25, 157
Sau96I GGNCC 1 cut(s) 476
ScrFI CCNGG 1 cut(s) 534
SduI GDGCHC 1 cut(s) 436
SfaNI GCATC 1 cut(s) 21
Sfr274I CTCGAG 1 cut(s) 494
SinI GGWCC 1 cut(s) 476
SlaI CTCGAG 1 cut(s) 494
SmlI CTYRAG 2 cut(s) 179, 494
SmoI CTYRAG 2 cut(s) 179, 494
Sse9I AATT 1 cut(s) 167
SspMI CTAG 2 cut(s) 29, 321
SstI GAGCTC 1 cut(s) 436
StyD4I CCNGG 1 cut(s) 532
TaaI ACNGT 1 cut(s) 235
TaiI ACGT 1 cut(s) 114
TaqI TCGA 4 cut(s) 24, 337, 495, 552
TasI AATT 1 cut(s) 167
TfiI GAWTC 2 cut(s) 554, 573
Tru1I TTAA 4 cut(s) 75, 359, 540, 625
Tru9I TTAA 4 cut(s) 75, 359, 540, 625
TscAI CASTG 1 cut(s) 238
TseFI GTSAC 2 cut(s) 235, 619
TseI GCWGC 3 cut(s) 287, 332, 604
Tsp45I GTSAC 2 cut(s) 235, 619
TspDTI ATGAA 1 cut(s) 626
TspRI CASTG 1 cut(s) 238
Van91I CCANNNNNTGG 1 cut(s) 533
VpaK11BI GGWCC 1 cut(s) 476
XbaI TCTAGA 1 cut(s) 28
XhoI CTCGAG 1 cut(s) 494
XspI CTAG 2 cut(s) 29, 321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.