Rh7DG045100

Endoplasmic reticulum membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0002194
Physical Location & Seq
Forward (+)
3425601 .. 3428085
2485 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG045100.1

Sequence Viewer

Length: 441 bp
ATGGGGGGAAGAGGATTGTTTGATCTTGAAAACCACTTTGCCTTCTATGGCGCATATCACAGCAACCCAGTAAACATTTTCATCCACACTCTGTTTGTGTGGCCAATTCTCTTCACCTCTCTTGTTCTTCTCTACTTCACACCTTCCCTGCTGAATTTCCCAGCTGGGGTTTATCATGGGTTTTTGTTTAATTTTGGGTTTGTTTTCACTGTGATCTATGCTTTGTATTATGTTGGATTGGATAAGAAAGCTGGGTCCTTGGCTGCTTTGCTCTGTGTTCTCTGCTGGGTTGGAGCGAGTGTTCTTGCTAGTCGCCTTGGGTTTTCTTTGGCATGGAAGGTTGTGTTGGCAGCTCAGTTGTTCTGTTGGACTGGACAGTTCATAGGCCATGGGGTGTTTGAGGTGACTAATTTTGTGGTTTTCAACTATTTGTTGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF962

Protein Analysis

146

Amino Acids

16.39

Weight (kDa)

7.76

Isoelectric Point (pI)

17.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mpo1-like PF06127 8 - 145 5.2e-16 2-hydroxy-palmitic acid dioxygenase Mpo1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 101
AcsI RAATTY 1 cut(s) 154
AfiI CCNNNNNNNGG 1 cut(s) 166
AgsI TTSAA 2 cut(s) 29, 424
AjuI GAANNNNNNNTTGG 2 cut(s) 329, 361
AloI GAACNNNNNNTCC 2 cut(s) 285, 317
AluBI AGCT 3 cut(s) 164, 251, 353
AluI AGCT 3 cut(s) 164, 251, 353
AoxI GGCC 2 cut(s) 101, 385
ApeKI GCWGC 2 cut(s) 263, 350
ApoI RAATTY 1 cut(s) 154
AspLEI GCGC 1 cut(s) 53
AspS9I GGNCC 1 cut(s) 255
AsuHPI GGTGA 2 cut(s) 106, 415
AvaII GGWCC 1 cut(s) 255
BalI TGGCCA 1 cut(s) 103
BbvI GCAGC 2 cut(s) 250, 362
BfaI CTAG 1 cut(s) 309
BisI GCNGC 2 cut(s) 264, 351
BlsI GCNGC 2 cut(s) 265, 352
Bme18I GGWCC 1 cut(s) 255
BmgT120I GGNCC 1 cut(s) 255
BmiI GGNNCC 1 cut(s) 256
BmrI ACTGGG 1 cut(s) 62
BmuI ACTGGG 1 cut(s) 62
BsaJI CCNNGG 3 cut(s) 258, 316, 388
BsaXI ACNNNNNCTCC 2 cut(s) 285, 315
Bsc4I CCNNNNNNNGG 1 cut(s) 166
Bse1I ACTGG 2 cut(s) 68, 376
BseDI CCNNGG 3 cut(s) 258, 316, 388
BseGI GGATG 1 cut(s) 81
BseLI CCNNNNNNNGG 1 cut(s) 166
BseMII CTCAG 1 cut(s) 368
BseNI ACTGG 2 cut(s) 68, 376
BseXI GCAGC 2 cut(s) 250, 362
BseYI CCCAGC 4 cut(s) 160, 164, 251, 285
BshFI GGCC 2 cut(s) 103, 387
BslI CCNNNNNNNGG 1 cut(s) 166
BsnI GGCC 2 cut(s) 103, 387
Bsp143I GATC 2 cut(s) 22, 213
Bsp19I CCATGG 1 cut(s) 388
BspANI GGCC 2 cut(s) 103, 387
BspCNI CTCAG 1 cut(s) 367
BspLI GGNNCC 1 cut(s) 256
BsrI ACTGG 2 cut(s) 68, 376
BssECI CCNNGG 3 cut(s) 258, 316, 388
BssMI GATC 2 cut(s) 22, 213
BssT1I CCWWGG 3 cut(s) 258, 316, 388
Bst4CI ACNGT 2 cut(s) 211, 378
Bst6I CTCTTC 2 cut(s) 4, 116
BstDEI CTNAG 1 cut(s) 354
BstDSI CCRYGG 1 cut(s) 388
BstF5I GGATG 1 cut(s) 81
BstHHI GCGC 1 cut(s) 53
BstKTI GATC 2 cut(s) 25, 216
BstMBI GATC 2 cut(s) 22, 213
BstV1I GCAGC 2 cut(s) 250, 362
BsuRI GGCC 2 cut(s) 103, 387
BtgI CCRYGG 1 cut(s) 388
BtsCI GGATG 1 cut(s) 81
BtsIMutI CAGTG 1 cut(s) 207
CfoI GCGC 1 cut(s) 53
Cfr13I GGNCC 1 cut(s) 255
CviAII CATG 3 cut(s) 176, 333, 389
CviJI RGCY 6 cut(s) 103, 164, 251, 263, 353, 387
CviKI_1 RGCY 6 cut(s) 103, 164, 251, 263, 353, 387
DdeI CTNAG 1 cut(s) 354
DpnI GATC 2 cut(s) 24, 215
DpnII GATC 2 cut(s) 22, 213
EaeI YGGCCR 1 cut(s) 101
Eam1104I CTCTTC 2 cut(s) 4, 116
EarI CTCTTC 2 cut(s) 4, 116
Eco130I CCWWGG 3 cut(s) 258, 316, 388
Eco47I GGWCC 1 cut(s) 255
EcoO109I RGGNCCY 1 cut(s) 255
EcoT14I CCWWGG 3 cut(s) 258, 316, 388
ErhI CCWWGG 3 cut(s) 258, 316, 388
FaeI CATG 3 cut(s) 179, 336, 392
FaiI YATR 8 cut(s) 48, 55, 177, 219, 231, 334, 383, 390
FatI CATG 3 cut(s) 175, 332, 388
Fnu4HI GCNGC 2 cut(s) 264, 351
FokI GGATG 1 cut(s) 68
Fsp4HI GCNGC 2 cut(s) 264, 351
FspBI CTAG 1 cut(s) 309
GlaI GCGC 1 cut(s) 52
GluI GCNGC 2 cut(s) 264, 351
GsaI CCCAGC 4 cut(s) 164, 168, 255, 289
HaeIII GGCC 2 cut(s) 103, 387
HhaI GCGC 1 cut(s) 53
Hin1II CATG 3 cut(s) 179, 336, 392
Hin6I GCGC 1 cut(s) 51
HinP1I GCGC 1 cut(s) 51
HphI GGTGA 2 cut(s) 106, 415
Hpy166II GTNNAC 1 cut(s) 73
Hpy188III TCNNGA 1 cut(s) 26
Hpy8I GTNNAC 1 cut(s) 73
HpyAV CCTTC 3 cut(s) 52, 153, 331
HpyCH4III ACNGT 2 cut(s) 211, 378
HpyF3I CTNAG 1 cut(s) 354
Hsp92II CATG 3 cut(s) 179, 336, 392
HspAI GCGC 1 cut(s) 51
Kzo9I GATC 2 cut(s) 22, 213
LmnI GCTCC 1 cut(s) 293
LpnPI CCDG 7 cut(s) 81, 150, 161, 174, 237, 271, 357
Lsp1109I GCAGC 2 cut(s) 250, 362
MaeI CTAG 1 cut(s) 309
MaeIII GTNAC 1 cut(s) 403
MalI GATC 2 cut(s) 24, 215
MboI GATC 2 cut(s) 22, 213
MboII GAAGA 3 cut(s) 21, 103, 119
MlsI TGGCCA 1 cut(s) 103
MluCI AATT 4 cut(s) 105, 154, 190, 409
MluNI TGGCCA 1 cut(s) 103
MmeI TCCRAC 3 cut(s) 214, 271, 347
MnlI CCTC 3 cut(s) 5, 127, 394
Mox20I TGGCCA 1 cut(s) 103
MscI TGGCCA 1 cut(s) 103
MseI TTAA 2 cut(s) 189, 439
Msp20I TGGCCA 1 cut(s) 103
MspA1I CMGCKG 1 cut(s) 164
NcoI CCATGG 1 cut(s) 388
NdeII GATC 2 cut(s) 22, 213
NlaIII CATG 3 cut(s) 179, 336, 392
NlaIV GGNNCC 1 cut(s) 256
NmuCI GTSAC 1 cut(s) 403
PkrI GCNGC 2 cut(s) 265, 352
PpuMI RGGWCCY 1 cut(s) 255
Psp5II RGGWCCY 1 cut(s) 255
PspFI CCCAGC 4 cut(s) 160, 164, 251, 285
PspN4I GGNNCC 1 cut(s) 256
PspPI GGNCC 1 cut(s) 255
PspPPI RGGWCCY 1 cut(s) 255
PvuII CAGCTG 1 cut(s) 164
SaqAI TTAA 2 cut(s) 189, 439
SatI GCNGC 2 cut(s) 264, 351
Sau3AI GATC 2 cut(s) 22, 213
Sau96I GGNCC 1 cut(s) 255
SetI ASST 7 cut(s) 119, 145, 166, 253, 342, 355, 405
SinI GGWCC 1 cut(s) 255
Sse9I AATT 4 cut(s) 105, 154, 190, 409
SspMI CTAG 1 cut(s) 309
StyI CCWWGG 3 cut(s) 258, 316, 388
TaaI ACNGT 2 cut(s) 211, 378
TasI AATT 4 cut(s) 105, 154, 190, 409
Tru1I TTAA 2 cut(s) 189, 439
Tru9I TTAA 2 cut(s) 189, 439
TscAI CASTG 1 cut(s) 214
TseFI GTSAC 1 cut(s) 403
TseI GCWGC 2 cut(s) 263, 350
Tsp45I GTSAC 1 cut(s) 403
TspDTI ATGAA 2 cut(s) 70, 370
TspRI CASTG 1 cut(s) 214
VpaK11BI GGWCC 1 cut(s) 255
XapI RAATTY 1 cut(s) 154
XspI CTAG 1 cut(s) 309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.