pycom05g19380

Endoplasmic reticulum membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0002194
Physical Location & Seq
Reverse (-)
22309755 .. 22310348
594 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g19380.1

Sequence Viewer

Length: 594 bp
ATGGGGAGGACTGGATTGTTTGATCTTGAAAGGCACTTCGCCTTCTATGGGGCGTATCACAGCAACCCAGTTAACATTTTGATTCACACTTTCTTTGTGTGGCCATTATTCTTCACCGCTGCTGTTCTTTTCCACTTCACACCCTCTCTCTACAATTTCTCATCTGGGTTTAACAATCATGGTTTCAAATTGAATTTAGGGTTTGTTTTAACTTTTATGTATGCTTTCTTTTATGTCAAATTGGACAAGAAAGCTGGGTCCTTGGCAGCTTTGCTATGTTTTCTCTGCTGGGTTGGAGCCAATGTTCTTGCTAGTGGGCTAGGGTTTGCTAAGTCATGGAAGCTTGTTCTTGCTGCTCAGGTTTTCTGCTGGGGTGGACAGTTCCTAGGCCACGGGATTTTTGAAAAAAGAGCACCAGCTCTTGCAGACAACTTTGCTCAAGCACTTCTGATGGGGCCTTACTTCGTGTTACTTGAGCTTCTTCAATCAGCCTTTGGGTATGAACCTTATCCAGGATTTCATAAAAGTGTGAAAGCAAAGATTGAAGCTAATATCAAAGAGTGGAAGGTCAAGAACCGAAAGAAACTCTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF962

Protein Analysis

198

Amino Acids

22.25

Weight (kDa)

9.6

Isoelectric Point (pI)

28.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mpo1-like PF06127 8 - 162 5.6e-23 2-hydroxy-palmitic acid dioxygenase Mpo1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 117
AcoI YGGCCR 1 cut(s) 101
AcsI RAATTY 1 cut(s) 193
AfiI CCNNNNNNNGG 2 cut(s) 48, 512
AgsI TTSAA 6 cut(s) 29, 187, 193, 404, 485, 545
AjnI CCWGG 1 cut(s) 511
AloI GAACNNNNNNTCC 2 cut(s) 288, 320
AluBI AGCT 6 cut(s) 254, 269, 343, 419, 478, 548
AluI AGCT 6 cut(s) 254, 269, 343, 419, 478, 548
Alw21I GWGCWC 1 cut(s) 415
AoxI GGCC 3 cut(s) 101, 388, 455
ApeKI GCWGC 3 cut(s) 119, 266, 353
ApoI RAATTY 1 cut(s) 193
AspA2I CCTAGG 1 cut(s) 385
AspS9I GGNCC 2 cut(s) 258, 455
AsuHPI GGTGA 1 cut(s) 106
AvaII GGWCC 1 cut(s) 258
AvrII CCTAGG 1 cut(s) 385
BalI TGGCCA 1 cut(s) 103
Bbv12I GWGCWC 1 cut(s) 415
BbvI GCAGC 3 cut(s) 106, 278, 340
BccI CCATC 1 cut(s) 445
BciT130I CCWGG 1 cut(s) 513
BfaI CTAG 3 cut(s) 312, 320, 386
BisI GCNGC 3 cut(s) 120, 267, 354
BlnI CCTAGG 1 cut(s) 385
BlsI GCNGC 3 cut(s) 121, 268, 355
Bme1390I CCNGG 1 cut(s) 513
Bme18I GGWCC 1 cut(s) 258
BmgT120I GGNCC 2 cut(s) 258, 455
BmiI GGNNCC 3 cut(s) 259, 298, 456
BmrFI CCNGG 1 cut(s) 513
BmrI ACTGGG 1 cut(s) 62
BmuI ACTGGG 1 cut(s) 62
Bpu10I CCTNAGC 1 cut(s) 357
BpuEI CTTGAG 2 cut(s) 423, 494
BsaJI CCNNGG 3 cut(s) 261, 385, 391
BsaXI ACNNNNNCTCC 2 cut(s) 288, 318
Bsc4I CCNNNNNNNGG 2 cut(s) 48, 512
Bse1I ACTGG 2 cut(s) 16, 68
BseBI CCWGG 1 cut(s) 513
BseDI CCNNGG 3 cut(s) 261, 385, 391
BseLI CCNNNNNNNGG 2 cut(s) 48, 512
BseMII CTCAG 1 cut(s) 371
BseNI ACTGG 2 cut(s) 16, 68
BseXI GCAGC 3 cut(s) 106, 278, 340
BseYI CCCAGC 3 cut(s) 254, 288, 369
BshFI GGCC 3 cut(s) 103, 390, 457
BsiHKAI GWGCWC 1 cut(s) 415
BslI CCNNNNNNNGG 2 cut(s) 48, 512
BsnI GGCC 3 cut(s) 103, 390, 457
Bsp1286I GDGCHC 1 cut(s) 415
Bsp143I GATC 1 cut(s) 22
BspACI CCGC 1 cut(s) 117
BspANI GGCC 3 cut(s) 103, 390, 457
BspCNI CTCAG 1 cut(s) 370
BspLI GGNNCC 3 cut(s) 259, 298, 456
BsrI ACTGG 2 cut(s) 16, 68
BssECI CCNNGG 3 cut(s) 261, 385, 391
BssMI GATC 1 cut(s) 22
BssT1I CCWWGG 2 cut(s) 261, 385
Bst2UI CCWGG 1 cut(s) 513
Bst4CI ACNGT 1 cut(s) 381
BstDEI CTNAG 2 cut(s) 330, 357
BstDSI CCRYGG 1 cut(s) 391
BstENI CCTNNNNNAGG 1 cut(s) 510
BstKTI GATC 1 cut(s) 25
BstMBI GATC 1 cut(s) 22
BstNI CCWGG 1 cut(s) 513
BstSCI CCNGG 1 cut(s) 511
BstV1I GCAGC 3 cut(s) 106, 278, 340
BsuRI GGCC 3 cut(s) 103, 390, 457
BtgI CCRYGG 1 cut(s) 391
Cfr13I GGNCC 2 cut(s) 258, 455
CviAII CATG 2 cut(s) 179, 336
DdeI CTNAG 2 cut(s) 330, 357
DpnI GATC 1 cut(s) 24
DpnII GATC 1 cut(s) 22
EaeI YGGCCR 1 cut(s) 101
Eco130I CCWWGG 2 cut(s) 261, 385
Eco47I GGWCC 1 cut(s) 258
EcoNI CCTNNNNNAGG 1 cut(s) 510
EcoO109I RGGNCCY 2 cut(s) 258, 455
EcoRII CCWGG 1 cut(s) 511
EcoT14I CCWWGG 2 cut(s) 261, 385
ErhI CCWWGG 2 cut(s) 261, 385
FaeI CATG 2 cut(s) 182, 339
FaiI YATR 9 cut(s) 48, 180, 218, 222, 234, 277, 337, 501, 522
FatI CATG 2 cut(s) 178, 335
Fnu4HI GCNGC 3 cut(s) 120, 267, 354
Fsp4HI GCNGC 3 cut(s) 120, 267, 354
FspBI CTAG 3 cut(s) 312, 320, 386
GluI GCNGC 3 cut(s) 120, 267, 354
GsaI CCCAGC 3 cut(s) 258, 292, 373
HaeIII GGCC 3 cut(s) 103, 390, 457
Hin1II CATG 2 cut(s) 182, 339
HincII GTYRAC 1 cut(s) 73
HindII GTYRAC 1 cut(s) 73
HindIII AAGCTT 1 cut(s) 341
HinfI GANTC 1 cut(s) 82
HpaI GTTAAC 1 cut(s) 73
HphI GGTGA 1 cut(s) 106
Hpy166II GTNNAC 2 cut(s) 73, 377
Hpy188I TCNGA 1 cut(s) 450
Hpy188III TCNNGA 3 cut(s) 26, 571, 591
Hpy8I GTNNAC 2 cut(s) 73, 377
HpyAV CCTTC 2 cut(s) 52, 559
HpyCH4III ACNGT 1 cut(s) 381
HpyCH4V TGCA 1 cut(s) 425
HpyF3I CTNAG 2 cut(s) 330, 357
Hsp92II CATG 2 cut(s) 182, 339
KspAI GTTAAC 1 cut(s) 73
Kzo9I GATC 1 cut(s) 22
LmnI GCTCC 1 cut(s) 296
LpnPI CCDG 9 cut(s) 81, 150, 240, 274, 344, 355, 429, 498, 525
Lsp1109I GCAGC 3 cut(s) 106, 278, 340
MaeI CTAG 3 cut(s) 312, 320, 386
MaeIII GTNAC 1 cut(s) 468
MalI GATC 1 cut(s) 24
MboI GATC 1 cut(s) 22
MboII GAAGA 2 cut(s) 103, 473
MhlI GDGCHC 1 cut(s) 415
MlsI TGGCCA 1 cut(s) 103
MluCI AATT 4 cut(s) 154, 188, 193, 239
MluNI TGGCCA 1 cut(s) 103
MmeI TCCRAC 1 cut(s) 274
MnlI CCTC 1 cut(s) 154
Mox20I TGGCCA 1 cut(s) 103
MscI TGGCCA 1 cut(s) 103
MseI TTAA 3 cut(s) 72, 171, 209
MslI CAYNNNNRTG 1 cut(s) 525
Msp20I TGGCCA 1 cut(s) 103
MspA1I CMGCKG 1 cut(s) 119
MspR9I CCNGG 1 cut(s) 513
MvaI CCWGG 1 cut(s) 513
NdeII GATC 1 cut(s) 22
NlaIII CATG 2 cut(s) 182, 339
NlaIV GGNNCC 3 cut(s) 259, 298, 456
PfeI GAWTC 1 cut(s) 82
PfoI TCCNGGA 1 cut(s) 511
PkrI GCNGC 3 cut(s) 121, 268, 355
PpuMI RGGWCCY 1 cut(s) 258
Psp5II RGGWCCY 1 cut(s) 258
Psp6I CCWGG 1 cut(s) 511
PspFI CCCAGC 3 cut(s) 254, 288, 369
PspGI CCWGG 1 cut(s) 511
PspN4I GGNNCC 3 cut(s) 259, 298, 456
PspPI GGNCC 2 cut(s) 258, 455
PspPPI RGGWCCY 1 cut(s) 258
RseI CAYNNNNRTG 1 cut(s) 525
SaqAI TTAA 3 cut(s) 72, 171, 209
SatI GCNGC 3 cut(s) 120, 267, 354
Sau3AI GATC 1 cut(s) 22
Sau96I GGNCC 2 cut(s) 258, 455
ScrFI CCNGG 1 cut(s) 513
SduI GDGCHC 1 cut(s) 415
SetI ASST 9 cut(s) 256, 271, 345, 363, 421, 480, 508, 550, 570
SinI GGWCC 1 cut(s) 258
SmiMI CAYNNNNRTG 1 cut(s) 525
SmlI CTYRAG 2 cut(s) 438, 473
SmoI CTYRAG 2 cut(s) 438, 473
Sse9I AATT 4 cut(s) 154, 188, 193, 239
SsiI CCGC 1 cut(s) 117
SspMI CTAG 3 cut(s) 312, 320, 386
StyD4I CCNGG 1 cut(s) 511
StyI CCWWGG 2 cut(s) 261, 385
TaaI ACNGT 1 cut(s) 381
TasI AATT 4 cut(s) 154, 188, 193, 239
TfiI GAWTC 1 cut(s) 82
Tru1I TTAA 3 cut(s) 72, 171, 209
Tru9I TTAA 3 cut(s) 72, 171, 209
TseI GCWGC 3 cut(s) 119, 266, 353
TspDTI ATGAA 2 cut(s) 509, 516
VpaK11BI GGWCC 1 cut(s) 258
XagI CCTNNNNNAGG 1 cut(s) 510
XapI RAATTY 1 cut(s) 193
XmaJI CCTAGG 1 cut(s) 385
XspI CTAG 3 cut(s) 312, 320, 386
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.