MD05G1207900.v1.1

Endoplasmic reticulum membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0002194
Physical Location & Seq
Reverse (-)
33915228 .. 33915821
594 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1207900.v1.1.491

Sequence Viewer

Length: 594 bp
ATGGGGAGGACCGGATTGTTTGATCTTGAAGGGCACTTCGCCTTCTATGGGGCATATCACAGCAACCCAGTTAACATTTTGATTCACACTTTCTTTGTGTGGCCATTATTCTTCACCGCTGCTGTTCTTTTCCACTTTACACCCTCTCTCTACAATTTCTCATCTGGGTTTAATAATCATGGTTTCACATTGAATTTAGGGTTTGTTTTAACTTTTATGTATGCTTTCTTTTATGTCAAATTGGACAAGAAAGCTGGGTCCTTGGCAGCTTTGCTATGTTTTCTCTGCTGGGTTGGAGCCAGTGTTCTTGCTAGTGGGCTAGGGTTTGTTAAGTCATGGAAGCTTGTTCTTGCTGCTCAGGTTTTCTGTTGGGGTGGACAGTTCATAGGCCACGGGATTTTTGAAAAAAGAGGACCAGCTCTTGCAGACAACTTTGCTCAAGCACTTCTGATGGGGCCTTACTTCGTGTTGCTTGAGCTTCTTCAATCAGCCTTTGGGTATGAACCTTATCCAGGGTTTCATAAAAGTGTGAGAGCAAAGATTGAAGTTAATATCAAAGAGTGGAAGGTCAAGAACCAAAAGAAACTCTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF962

Protein Analysis

198

Amino Acids

22.14

Weight (kDa)

9.36

Isoelectric Point (pI)

27.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mpo1-like PF06127 9 - 162 1.6e-22 2-hydroxy-palmitic acid dioxygenase Mpo1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 117
AcoI YGGCCR 1 cut(s) 101
AcsI RAATTY 1 cut(s) 193
AfiI CCNNNNNNNGG 2 cut(s) 48, 512
AgsI TTSAA 5 cut(s) 29, 193, 404, 485, 545
AjnI CCWGG 1 cut(s) 511
AloI GAACNNNNNNTCC 2 cut(s) 288, 320
AluBI AGCT 5 cut(s) 254, 269, 343, 419, 478
AluI AGCT 5 cut(s) 254, 269, 343, 419, 478
AoxI GGCC 3 cut(s) 101, 388, 455
ApeKI GCWGC 3 cut(s) 119, 266, 353
ApoI RAATTY 1 cut(s) 193
AspS9I GGNCC 4 cut(s) 9, 258, 413, 455
AsuHPI GGTGA 1 cut(s) 106
AvaII GGWCC 3 cut(s) 9, 258, 413
BaeGI GKGCMC 1 cut(s) 36
BalI TGGCCA 1 cut(s) 103
BbvI GCAGC 3 cut(s) 106, 278, 340
BccI CCATC 1 cut(s) 445
BciT130I CCWGG 1 cut(s) 513
BfaI CTAG 2 cut(s) 312, 320
BisI GCNGC 3 cut(s) 120, 267, 354
BlsI GCNGC 3 cut(s) 121, 268, 355
Bme1390I CCNGG 1 cut(s) 513
Bme18I GGWCC 3 cut(s) 9, 258, 413
BmgT120I GGNCC 4 cut(s) 9, 258, 413, 455
BmiI GGNNCC 3 cut(s) 259, 298, 456
BmrFI CCNGG 1 cut(s) 513
BmrI ACTGGG 1 cut(s) 62
BmuI ACTGGG 1 cut(s) 62
Bpu10I CCTNAGC 1 cut(s) 357
BpuEI CTTGAG 2 cut(s) 423, 494
BsaJI CCNNGG 3 cut(s) 261, 391, 512
BsaWI WCCGGW 1 cut(s) 11
BsaXI ACNNNNNCTCC 2 cut(s) 288, 318
Bsc4I CCNNNNNNNGG 2 cut(s) 48, 512
Bse1I ACTGG 2 cut(s) 68, 300
BseBI CCWGG 1 cut(s) 513
BseDI CCNNGG 3 cut(s) 261, 391, 512
BseLI CCNNNNNNNGG 2 cut(s) 48, 512
BseMII CTCAG 1 cut(s) 371
BseNI ACTGG 2 cut(s) 68, 300
BseSI GKGCMC 1 cut(s) 36
BseXI GCAGC 3 cut(s) 106, 278, 340
BseYI CCCAGC 2 cut(s) 254, 288
BshFI GGCC 3 cut(s) 103, 390, 457
BsiSI CCGG 1 cut(s) 12
BslI CCNNNNNNNGG 2 cut(s) 48, 512
BsnI GGCC 3 cut(s) 103, 390, 457
Bsp1286I GDGCHC 1 cut(s) 36
Bsp143I GATC 1 cut(s) 22
BspACI CCGC 1 cut(s) 117
BspANI GGCC 3 cut(s) 103, 390, 457
BspCNI CTCAG 1 cut(s) 370
BspLI GGNNCC 3 cut(s) 259, 298, 456
BsrI ACTGG 2 cut(s) 68, 300
BssECI CCNNGG 3 cut(s) 261, 391, 512
BssMI GATC 1 cut(s) 22
BssT1I CCWWGG 1 cut(s) 261
Bst2UI CCWGG 1 cut(s) 513
Bst4CI ACNGT 1 cut(s) 381
BstDEI CTNAG 1 cut(s) 357
BstDSI CCRYGG 1 cut(s) 391
BstENI CCTNNNNNAGG 1 cut(s) 510
BstKTI GATC 1 cut(s) 25
BstMBI GATC 1 cut(s) 22
BstNI CCWGG 1 cut(s) 513
BstSCI CCNGG 1 cut(s) 511
BstSLI GKGCMC 1 cut(s) 36
BstV1I GCAGC 3 cut(s) 106, 278, 340
BsuRI GGCC 3 cut(s) 103, 390, 457
BtgI CCRYGG 1 cut(s) 391
BtsIMutI CAGTG 1 cut(s) 307
Cfr13I GGNCC 4 cut(s) 9, 258, 413, 455
CviAII CATG 2 cut(s) 179, 336
DdeI CTNAG 1 cut(s) 357
DpnI GATC 1 cut(s) 24
DpnII GATC 1 cut(s) 22
EaeI YGGCCR 1 cut(s) 101
Eco130I CCWWGG 1 cut(s) 261
Eco47I GGWCC 3 cut(s) 9, 258, 413
EcoNI CCTNNNNNAGG 1 cut(s) 510
EcoO109I RGGNCCY 2 cut(s) 258, 455
EcoRII CCWGG 1 cut(s) 511
EcoT14I CCWWGG 1 cut(s) 261
ErhI CCWWGG 1 cut(s) 261
FaeI CATG 2 cut(s) 182, 339
FatI CATG 2 cut(s) 178, 335
Fnu4HI GCNGC 3 cut(s) 120, 267, 354
Fsp4HI GCNGC 3 cut(s) 120, 267, 354
FspBI CTAG 2 cut(s) 312, 320
GluI GCNGC 3 cut(s) 120, 267, 354
GsaI CCCAGC 2 cut(s) 258, 292
HaeIII GGCC 3 cut(s) 103, 390, 457
HapII CCGG 1 cut(s) 12
Hin1II CATG 2 cut(s) 182, 339
HincII GTYRAC 1 cut(s) 73
HindII GTYRAC 1 cut(s) 73
HindIII AAGCTT 1 cut(s) 341
HinfI GANTC 1 cut(s) 82
HpaI GTTAAC 1 cut(s) 73
HpaII CCGG 1 cut(s) 12
HphI GGTGA 1 cut(s) 106
Hpy166II GTNNAC 2 cut(s) 73, 377
Hpy188I TCNGA 1 cut(s) 450
Hpy188III TCNNGA 3 cut(s) 26, 571, 591
Hpy8I GTNNAC 2 cut(s) 73, 377
HpyAV CCTTC 3 cut(s) 23, 52, 559
HpyCH4III ACNGT 1 cut(s) 381
HpyCH4V TGCA 1 cut(s) 425
HpyF3I CTNAG 1 cut(s) 357
Hsp92II CATG 2 cut(s) 182, 339
KspAI GTTAAC 1 cut(s) 73
Kzo9I GATC 1 cut(s) 22
LmnI GCTCC 1 cut(s) 296
Lsp1109I GCAGC 3 cut(s) 106, 278, 340
MaeI CTAG 2 cut(s) 312, 320
MalI GATC 1 cut(s) 24
MboI GATC 1 cut(s) 22
MboII GAAGA 2 cut(s) 103, 473
MhlI GDGCHC 1 cut(s) 36
MlsI TGGCCA 1 cut(s) 103
MluCI AATT 3 cut(s) 154, 193, 239
MluNI TGGCCA 1 cut(s) 103
MmeI TCCRAC 1 cut(s) 274
MnlI CCTC 2 cut(s) 154, 404
Mox20I TGGCCA 1 cut(s) 103
MscI TGGCCA 1 cut(s) 103
MseI TTAA 5 cut(s) 72, 171, 209, 330, 549
MslI CAYNNNNRTG 1 cut(s) 525
Msp20I TGGCCA 1 cut(s) 103
MspA1I CMGCKG 1 cut(s) 119
MspI CCGG 1 cut(s) 12
MspR9I CCNGG 1 cut(s) 513
MvaI CCWGG 1 cut(s) 513
NdeII GATC 1 cut(s) 22
NlaIII CATG 2 cut(s) 182, 339
NlaIV GGNNCC 3 cut(s) 259, 298, 456
PfeI GAWTC 1 cut(s) 82
PkrI GCNGC 3 cut(s) 121, 268, 355
PpuMI RGGWCCY 1 cut(s) 258
Psp5II RGGWCCY 1 cut(s) 258
Psp6I CCWGG 1 cut(s) 511
PspFI CCCAGC 2 cut(s) 254, 288
PspGI CCWGG 1 cut(s) 511
PspN4I GGNNCC 3 cut(s) 259, 298, 456
PspPI GGNCC 4 cut(s) 9, 258, 413, 455
PspPPI RGGWCCY 1 cut(s) 258
RseI CAYNNNNRTG 1 cut(s) 525
SaqAI TTAA 5 cut(s) 72, 171, 209, 330, 549
SatI GCNGC 3 cut(s) 120, 267, 354
Sau3AI GATC 1 cut(s) 22
Sau96I GGNCC 4 cut(s) 9, 258, 413, 455
ScrFI CCNGG 1 cut(s) 513
SduI GDGCHC 1 cut(s) 36
SetI ASST 8 cut(s) 256, 271, 345, 363, 421, 480, 508, 570
SinI GGWCC 3 cut(s) 9, 258, 413
SmiMI CAYNNNNRTG 1 cut(s) 525
SmlI CTYRAG 2 cut(s) 438, 473
SmoI CTYRAG 2 cut(s) 438, 473
Sse9I AATT 3 cut(s) 154, 193, 239
SsiI CCGC 1 cut(s) 117
SspMI CTAG 2 cut(s) 312, 320
StyD4I CCNGG 1 cut(s) 511
StyI CCWWGG 1 cut(s) 261
TaaI ACNGT 1 cut(s) 381
TasI AATT 3 cut(s) 154, 193, 239
TfiI GAWTC 1 cut(s) 82
Tru1I TTAA 5 cut(s) 72, 171, 209, 330, 549
Tru9I TTAA 5 cut(s) 72, 171, 209, 330, 549
TscAI CASTG 1 cut(s) 307
TseI GCWGC 3 cut(s) 119, 266, 353
TspDTI ATGAA 3 cut(s) 373, 509, 516
TspRI CASTG 1 cut(s) 307
VpaK11BI GGWCC 3 cut(s) 9, 258, 413
XagI CCTNNNNNAGG 1 cut(s) 510
XapI RAATTY 1 cut(s) 193
XspI CTAG 2 cut(s) 312, 320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.