MD16G1069800.v1.1

Endoplasmic reticulum membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0002194
Physical Location & Seq
Reverse (-)
4910691 .. 4912269
1579 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1069800.v1.1.491

Sequence Viewer

Length: 600 bp
ATGGGGAAGACCGGACTGTTTGATCTCGAAAAGCACTTTGCTTTCTATGGAGCTTACCACAGCAACCCGGTCAACATAGCGATTCATATGCTCTTCGTCTGGCCTATCCTCTTCACCGCTCTGCTCATCCTTTACTTCACGCCCTCGCTGCTCAGTTTTGGGGTTTCGGTGTTTGGTAACGACGTCGCTCTGCCTTTCAACGTCGGCCTGCTGTTGACTATAATCTATTCGGCGTTTTACATCTGTTTGGACGCAAAAGCTGGCTCCTTGGGTGCTCTGCTCTGCGTACTTTGCTGGGTTGGTAGCTGTTTCCTTGGAACCCTACTCGGATTTTCCATCTCTTGGAAGGTTGTTCTGGTGGCTCAGATAGTGTGTTGGACTGGACAATTTATCGGCCATGGAGTCTTTGAGAAAAGGGCACCAGCTTTGTTGGACAATCTTGCACAAGCCTTTATCATGGCTCCCTTCTTTGTTCTGTTAGAGGCTCTGCAGATCTTCTTCGGGTACGAACCGTACCCCGGGTTTCAAGCAATTGTTCAAGCAAAGATTGATGCTGAAATCAGTGAATGGCAAGAGAATAAGAAGAAACTGATCTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF962

Protein Analysis

200

Amino Acids

22.05

Weight (kDa)

6.04

Isoelectric Point (pI)

30.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mpo1-like PF06127 8 - 163 1.3e-23 2-hydroxy-palmitic acid dioxygenase Mpo1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 186
AccB1I GGYRCC 1 cut(s) 418
AccB7I CCANNNNNTGG 1 cut(s) 342
AccBSI CCGCTC 1 cut(s) 119
AciI CCGC 1 cut(s) 117
AcoI YGGCCR 1 cut(s) 394
AcyI GRCGYC 1 cut(s) 183
AfaI GTAC 3 cut(s) 288, 506, 515
AfiI CCNNNNNNNGG 2 cut(s) 342, 518
AgsI TTSAA 3 cut(s) 199, 527, 539
AluBI AGCT 4 cut(s) 53, 260, 306, 425
AluI AGCT 4 cut(s) 53, 260, 306, 425
Alw21I GWGCWC 1 cut(s) 277
Ama87I CYCGRG 1 cut(s) 518
AoxI GGCC 3 cut(s) 101, 205, 394
ApeKI GCWGC 1 cut(s) 148
AsuC2I CCSGG 3 cut(s) 68, 519, 520
AsuHPI GGTGA 1 cut(s) 106
AvaI CYCGRG 1 cut(s) 518
BaeGI GKGCMC 1 cut(s) 421
BanI GGYRCC 1 cut(s) 418
BbsI GAAGAC 1 cut(s) 14
Bbv12I GWGCWC 1 cut(s) 277
BbvI GCAGC 1 cut(s) 135
BccI CCATC 1 cut(s) 344
BcgI CGANNNNNNTGC 2 cut(s) 70, 104
BcnI CCSGG 3 cut(s) 68, 519, 520
BfmI CTRYAG 1 cut(s) 488
BglII AGATCT 1 cut(s) 492
BisI GCNGC 1 cut(s) 149
BlsI GCNGC 1 cut(s) 150
Bme1390I CCNGG 3 cut(s) 68, 519, 520
BmeT110I CYCGRG 1 cut(s) 518
BmiI GGNNCC 4 cut(s) 265, 319, 420, 462
BmrFI CCNGG 3 cut(s) 68, 519, 520
BmsI GCATC 1 cut(s) 541
BpiI GAAGAC 1 cut(s) 14
BpuMI CCSGG 3 cut(s) 68, 519, 520
BsaHI GRCGYC 1 cut(s) 183
BsaJI CCNNGG 5 cut(s) 267, 313, 397, 517, 518
BsaWI WCCGGW 1 cut(s) 11
Bsc4I CCNNNNNNNGG 2 cut(s) 342, 518
Bse1I ACTGG 1 cut(s) 385
BseDI CCNNGG 5 cut(s) 267, 313, 397, 517, 518
BseGI GGATG 1 cut(s) 126
BseLI CCNNNNNNNGG 2 cut(s) 342, 518
BseMII CTCAG 2 cut(s) 166, 377
BseNI ACTGG 1 cut(s) 385
BseSI GKGCMC 1 cut(s) 421
BseXI GCAGC 1 cut(s) 135
BseYI CCCAGC 1 cut(s) 294
BshFI GGCC 3 cut(s) 103, 207, 396
BshNI GGYRCC 1 cut(s) 418
BsiHKAI GWGCWC 1 cut(s) 277
BsiHKCI CYCGRG 1 cut(s) 518
BsiSI CCGG 3 cut(s) 12, 68, 519
BslI CCNNNNNNNGG 2 cut(s) 342, 518
BsnI GGCC 3 cut(s) 103, 207, 396
BsoBI CYCGRG 1 cut(s) 518
Bsp1286I GDGCHC 2 cut(s) 277, 421
Bsp143I GATC 3 cut(s) 22, 492, 591
Bsp19I CCATGG 1 cut(s) 397
BspACI CCGC 1 cut(s) 117
BspANI GGCC 3 cut(s) 103, 207, 396
BspCNI CTCAG 2 cut(s) 165, 376
BspLI GGNNCC 4 cut(s) 265, 319, 420, 462
BspMAI CTGCAG 1 cut(s) 492
BspQI GCTCTTC 1 cut(s) 98
BspT107I GGYRCC 1 cut(s) 418
BsrBI CCGCTC 1 cut(s) 119
BsrI ACTGG 1 cut(s) 385
BssECI CCNNGG 5 cut(s) 267, 313, 397, 517, 518
BssMI GATC 3 cut(s) 22, 492, 591
BssNI GRCGYC 1 cut(s) 183
BssT1I CCWWGG 3 cut(s) 267, 313, 397
Bst4CI ACNGT 2 cut(s) 18, 513
Bst6I CTCTTC 2 cut(s) 98, 116
BstACI GRCGYC 1 cut(s) 183
BstC8I GCNNGC 2 cut(s) 209, 262
BstDEI CTNAG 2 cut(s) 152, 363
BstDSI CCRYGG 1 cut(s) 397
BstF5I GGATG 1 cut(s) 126
BstKTI GATC 3 cut(s) 25, 495, 594
BstMBI GATC 3 cut(s) 22, 492, 591
BstMWI GCNNNNNNNGC 2 cut(s) 148, 291
BstSCI CCNGG 3 cut(s) 66, 517, 518
BstSFI CTRYAG 1 cut(s) 488
BstSLI GKGCMC 1 cut(s) 421
BstV1I GCAGC 1 cut(s) 135
BstV2I GAAGAC 1 cut(s) 14
BstX2I RGATCY 1 cut(s) 492
BstYI RGATCY 1 cut(s) 492
BsuRI GGCC 3 cut(s) 103, 207, 396
BtgI CCRYGG 1 cut(s) 397
BtsCI GGATG 1 cut(s) 126
BtsIMutI CAGTG 1 cut(s) 568
Cac8I GCNNGC 2 cut(s) 209, 262
Cfr9I CCCGGG 1 cut(s) 518
CseI GACGC 1 cut(s) 260
Csp6I GTAC 3 cut(s) 287, 505, 514
CviAII CATG 2 cut(s) 398, 457
CviQI GTAC 3 cut(s) 287, 505, 514
DdeI CTNAG 2 cut(s) 152, 363
DpnI GATC 3 cut(s) 24, 494, 593
DpnII GATC 3 cut(s) 22, 492, 591
EaeI YGGCCR 1 cut(s) 394
Eam1104I CTCTTC 2 cut(s) 98, 116
EarI CTCTTC 2 cut(s) 98, 116
Eco130I CCWWGG 3 cut(s) 267, 313, 397
Eco88I CYCGRG 1 cut(s) 518
EcoT14I CCWWGG 3 cut(s) 267, 313, 397
ErhI CCWWGG 3 cut(s) 267, 313, 397
FaeI CATG 2 cut(s) 401, 460
FaiI YATR 7 cut(s) 48, 77, 87, 89, 221, 399, 458
FatI CATG 2 cut(s) 397, 456
FauNDI CATATG 1 cut(s) 87
Fnu4HI GCNGC 1 cut(s) 149
FokI GGATG 1 cut(s) 113
Fsp4HI GCNGC 1 cut(s) 149
GluI GCNGC 1 cut(s) 149
GsaI CCCAGC 1 cut(s) 298
HaeIII GGCC 3 cut(s) 103, 207, 396
HapII CCGG 3 cut(s) 12, 68, 519
HgaI GACGC 1 cut(s) 260
Hin1I GRCGYC 1 cut(s) 183
Hin1II CATG 2 cut(s) 401, 460
HincII GTYRAC 2 cut(s) 73, 216
HindII GTYRAC 2 cut(s) 73, 216
HinfI GANTC 2 cut(s) 82, 402
HpaII CCGG 3 cut(s) 12, 68, 519
HphI GGTGA 1 cut(s) 106
Hpy166II GTNNAC 2 cut(s) 73, 216
Hpy188I TCNGA 2 cut(s) 329, 366
Hpy188III TCNNGA 1 cut(s) 26
Hpy8I GTNNAC 2 cut(s) 73, 216
Hpy99I CGWCG 3 cut(s) 185, 188, 206
HpyAV CCTTC 2 cut(s) 340, 475
HpyCH4III ACNGT 2 cut(s) 18, 513
HpyCH4IV ACGT 2 cut(s) 183, 201
HpyCH4V TGCA 2 cut(s) 443, 490
HpyF10VI GCNNNNNNNGC 2 cut(s) 148, 291
HpyF3I CTNAG 2 cut(s) 152, 363
HpySE526I ACGT 2 cut(s) 183, 201
Hsp92I GRCGYC 1 cut(s) 183
Hsp92II CATG 2 cut(s) 401, 460
Kzo9I GATC 3 cut(s) 22, 492, 591
LguI GCTCTTC 1 cut(s) 98
LmnI GCTCC 3 cut(s) 50, 269, 466
Lsp1109I GCAGC 1 cut(s) 135
LweI GCATC 1 cut(s) 541
MaeII ACGT 2 cut(s) 183, 201
MaeIII GTNAC 1 cut(s) 176
MalI GATC 3 cut(s) 24, 494, 593
MbiI CCGCTC 1 cut(s) 119
MboI GATC 3 cut(s) 22, 492, 591
MboII GAAGA 6 cut(s) 19, 85, 103, 487, 490, 595
MfeI CAATTG 1 cut(s) 531
MflI RGATCY 1 cut(s) 492
MhlI GDGCHC 2 cut(s) 277, 421
MluCI AATT 2 cut(s) 386, 531
MlyI GAGTC 1 cut(s) 411
MmeI TCCRAC 2 cut(s) 356, 411
MnlI CCTC 3 cut(s) 119, 154, 475
MspI CCGG 3 cut(s) 12, 68, 519
MspR9I CCNGG 3 cut(s) 68, 519, 520
MunI CAATTG 1 cut(s) 531
MwoI GCNNNNNNNGC 2 cut(s) 148, 291
NciI CCSGG 3 cut(s) 68, 519, 520
NcoI CCATGG 1 cut(s) 397
NdeI CATATG 1 cut(s) 87
NdeII GATC 3 cut(s) 22, 492, 591
NlaIII CATG 2 cut(s) 401, 460
NlaIV GGNNCC 4 cut(s) 265, 319, 420, 462
PciSI GCTCTTC 1 cut(s) 98
PfeI GAWTC 1 cut(s) 82
PflMI CCANNNNNTGG 1 cut(s) 342
PkrI GCNGC 1 cut(s) 150
PleI GAGTC 1 cut(s) 410
PpsI GAGTC 1 cut(s) 410
PspFI CCCAGC 1 cut(s) 294
PspN4I GGNNCC 4 cut(s) 265, 319, 420, 462
PstI CTGCAG 1 cut(s) 492
PsuI RGATCY 1 cut(s) 492
RsaI GTAC 3 cut(s) 288, 506, 515
RsaNI GTAC 3 cut(s) 287, 505, 514
SapI GCTCTTC 1 cut(s) 98
SatI GCNGC 1 cut(s) 149
Sau3AI GATC 3 cut(s) 22, 492, 591
SchI GAGTC 1 cut(s) 411
ScrFI CCNGG 3 cut(s) 68, 519, 520
SduI GDGCHC 2 cut(s) 277, 421
SetI ASST 7 cut(s) 55, 186, 204, 262, 308, 351, 427
SfaNI GCATC 1 cut(s) 541
SfcI CTRYAG 1 cut(s) 488
SmaI CCCGGG 1 cut(s) 520
Sse9I AATT 2 cut(s) 386, 531
SsiI CCGC 1 cut(s) 117
StyD4I CCNGG 3 cut(s) 66, 517, 518
StyI CCWWGG 3 cut(s) 267, 313, 397
TaaI ACNGT 2 cut(s) 18, 513
TaiI ACGT 2 cut(s) 186, 204
TaqI TCGA 1 cut(s) 27
TasI AATT 2 cut(s) 386, 531
TfiI GAWTC 1 cut(s) 82
TscAI CASTG 1 cut(s) 568
TseI GCWGC 1 cut(s) 148
TspDTI ATGAA 1 cut(s) 74
TspMI CCCGGG 1 cut(s) 518
TspRI CASTG 1 cut(s) 568
Van91I CCANNNNNTGG 1 cut(s) 342
XmaI CCCGGG 1 cut(s) 518
ZraI GACGTC 1 cut(s) 184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.