FvH4_4g31090

Endoplasmic reticulum membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0002194
Physical Location & Seq
Reverse (-)
30391843 .. 30392832
990 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g31090.t1

Sequence Viewer

Length: 603 bp
ATGGGGAAGACTGGATTGTTCGACCTTGAGAAGCACTTCGCTTTCTATGGAGCTTATCACAGCAATCCGATCAACATAGCGATACATATGATCTTCGTCTGGCCCATCTTTTTCACAGCTCTGTTGATCCTCTATTTCACGCCGTCGATTTTCAACGTGGAGCTTTCACTGTTTGGGAGCAGCCATGTCGTTCTGGCTTTCAACGTCGGCTTCTTGCTGACTTTGATCTATTCGCTGTTCTACTTCTTCCTTGATGCTAAAGCTGGTTCCTTGGCTGCTCTGCTTTGCTTCATTTGCTGGGTCGGCAGTAGCTTCCTTGCGTGTCGACTCGGGTTTTCGCTTGCTTGGAAGGTTGTTCTGGTGGCTCAGATAGTGTGTTGGACTGGACAGTTTATTGGCCATGGGGTCTTTGAGAAAAGGGCACCGGCTTTGTTGGACAACCTTGCGCAGGCGTTTATAATGGCTCCTTTCTTTGTTTTGTTAGAGGCTCTGCAAAGCTTCTTTGATTATGAACCGTATCCGGGGTTTCATGCAATAGTTCAGGCGAAAGTTGATGCTGAAATTACTGACTGGAAAGATAAGAAGCAGAGACTGGTATCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF962

Protein Analysis

201

Amino Acids

22.58

Weight (kDa)

6.57

Isoelectric Point (pI)

30.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mpo1-like PF06127 8 - 165 1.7e-23 2-hydroxy-palmitic acid dioxygenase Mpo1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 458
Acc16I TGCGCA 1 cut(s) 447
AccB1I GGYRCC 1 cut(s) 421
AccI GTMKAC 1 cut(s) 325
AclWI GGATC 1 cut(s) 121
AcoI YGGCCR 1 cut(s) 397
AfiI CCNNNNNNNGG 2 cut(s) 448, 521
AgsI TTSAA 2 cut(s) 154, 202
AluBI AGCT 6 cut(s) 53, 119, 163, 263, 312, 498
AluI AGCT 6 cut(s) 53, 119, 163, 263, 312, 498
Alw26I GTCTC 1 cut(s) 583
AlwI GGATC 1 cut(s) 121
AlwNI CAGNNNCTG 1 cut(s) 592
Ama87I CYCGRG 1 cut(s) 329
AoxI GGCC 2 cut(s) 101, 397
ApeKI GCWGC 2 cut(s) 180, 275
Asp700I GAANNNNTTC 1 cut(s) 35
AspLEI GCGC 1 cut(s) 448
AspS9I GGNCC 1 cut(s) 102
AsuC2I CCSGG 1 cut(s) 522
AvaI CYCGRG 1 cut(s) 329
BaeGI GKGCMC 1 cut(s) 424
BalI TGGCCA 1 cut(s) 399
BanI GGYRCC 1 cut(s) 421
BbsI GAAGAC 1 cut(s) 14
BbvI GCAGC 2 cut(s) 192, 262
BccI CCATC 1 cut(s) 113
BceAI ACGGC 1 cut(s) 127
BcgI CGANNNNNNTGC 2 cut(s) 169, 203
BciVI GTATCC 1 cut(s) 528
BcnI CCSGG 1 cut(s) 522
BcoDI GTCTC 1 cut(s) 583
BfuI GTATCC 1 cut(s) 528
BisI GCNGC 2 cut(s) 181, 276
BlsI GCNGC 2 cut(s) 182, 277
Bme1390I CCNGG 1 cut(s) 522
BmeT110I CYCGRG 1 cut(s) 329
BmgT120I GGNCC 1 cut(s) 102
BmiI GGNNCC 3 cut(s) 268, 423, 465
BmrFI CCNGG 1 cut(s) 522
BmsI GCATC 2 cut(s) 244, 544
BpiI GAAGAC 1 cut(s) 14
BpuEI CTTGAG 1 cut(s) 47
BpuMI CCSGG 1 cut(s) 522
BsaJI CCNNGG 3 cut(s) 270, 400, 521
Bsc4I CCNNNNNNNGG 2 cut(s) 448, 521
Bse118I RCCGGY 1 cut(s) 424
Bse1I ACTGG 4 cut(s) 16, 388, 575, 597
BseDI CCNNGG 3 cut(s) 270, 400, 521
BseLI CCNNNNNNNGG 2 cut(s) 448, 521
BseMII CTCAG 1 cut(s) 380
BseNI ACTGG 4 cut(s) 16, 388, 575, 597
BseSI GKGCMC 1 cut(s) 424
BseXI GCAGC 2 cut(s) 192, 262
BseYI CCCAGC 1 cut(s) 297
BshFI GGCC 2 cut(s) 103, 399
BshNI GGYRCC 1 cut(s) 421
BsiHKCI CYCGRG 1 cut(s) 329
BsiSI CCGG 2 cut(s) 425, 521
BslI CCNNNNNNNGG 2 cut(s) 448, 521
BsmAI GTCTC 1 cut(s) 583
BsnI GGCC 2 cut(s) 103, 399
BsoBI CYCGRG 1 cut(s) 329
Bsp1286I GDGCHC 1 cut(s) 424
Bsp143I GATC 4 cut(s) 69, 90, 126, 225
Bsp19I CCATGG 1 cut(s) 400
BspANI GGCC 2 cut(s) 103, 399
BspCNI CTCAG 1 cut(s) 379
BspLI GGNNCC 3 cut(s) 268, 423, 465
BspPI GGATC 1 cut(s) 121
BspT107I GGYRCC 1 cut(s) 421
BsrFI RCCGGY 1 cut(s) 424
BsrI ACTGG 4 cut(s) 16, 388, 575, 597
BssAI RCCGGY 1 cut(s) 424
BssECI CCNNGG 3 cut(s) 270, 400, 521
BssMI GATC 4 cut(s) 69, 90, 126, 225
BssT1I CCWWGG 2 cut(s) 270, 400
Bst4CI ACNGT 3 cut(s) 171, 390, 516
BstC8I GCNNGC 2 cut(s) 342, 450
BstDEI CTNAG 1 cut(s) 366
BstDSI CCRYGG 1 cut(s) 400
BstENI CCTNNNNNAGG 1 cut(s) 446
BstHHI GCGC 1 cut(s) 448
BstKTI GATC 4 cut(s) 72, 93, 129, 228
BstMAI GTCTC 1 cut(s) 583
BstMBI GATC 4 cut(s) 69, 90, 126, 225
BstMWI GCNNNNNNNGC 2 cut(s) 294, 303
BstSCI CCNGG 1 cut(s) 520
BstSLI GKGCMC 1 cut(s) 424
BstV1I GCAGC 2 cut(s) 192, 262
BstV2I GAAGAC 1 cut(s) 14
BsuI GTATCC 1 cut(s) 528
BsuRI GGCC 2 cut(s) 103, 399
BtgI CCRYGG 1 cut(s) 400
BtsIMutI CAGTG 1 cut(s) 167
Cac8I GCNNGC 2 cut(s) 342, 450
CaiI CAGNNNCTG 1 cut(s) 592
CfoI GCGC 1 cut(s) 448
Cfr10I RCCGGY 1 cut(s) 424
Cfr13I GGNCC 1 cut(s) 102
CviAII CATG 3 cut(s) 185, 401, 530
DdeI CTNAG 1 cut(s) 366
DpnI GATC 4 cut(s) 71, 92, 128, 227
DpnII GATC 4 cut(s) 69, 90, 126, 225
EaeI YGGCCR 1 cut(s) 397
Eco130I CCWWGG 2 cut(s) 270, 400
Eco88I CYCGRG 1 cut(s) 329
EcoNI CCTNNNNNAGG 1 cut(s) 446
EcoT14I CCWWGG 2 cut(s) 270, 400
ErhI CCWWGG 2 cut(s) 270, 400
FaeI CATG 3 cut(s) 188, 404, 533
FatI CATG 3 cut(s) 184, 400, 529
FauNDI CATATG 1 cut(s) 87
FblI GTMKAC 1 cut(s) 325
Fnu4HI GCNGC 2 cut(s) 181, 276
Fsp4HI GCNGC 2 cut(s) 181, 276
FspI TGCGCA 1 cut(s) 447
GlaI GCGC 1 cut(s) 447
GluI GCNGC 2 cut(s) 181, 276
GsaI CCCAGC 1 cut(s) 301
HaeIII GGCC 2 cut(s) 103, 399
HapII CCGG 2 cut(s) 425, 521
HhaI GCGC 1 cut(s) 448
Hin1II CATG 3 cut(s) 188, 404, 533
Hin6I GCGC 1 cut(s) 446
HinP1I GCGC 1 cut(s) 446
HincII GTYRAC 1 cut(s) 326
HindII GTYRAC 1 cut(s) 326
HindIII AAGCTT 1 cut(s) 496
HinfI GANTC 1 cut(s) 327
HpaII CCGG 2 cut(s) 425, 521
Hpy166II GTNNAC 1 cut(s) 326
Hpy188I TCNGA 2 cut(s) 69, 369
Hpy8I GTNNAC 1 cut(s) 326
Hpy99I CGWCG 2 cut(s) 148, 209
HpyAV CCTTC 1 cut(s) 343
HpyCH4III ACNGT 3 cut(s) 171, 390, 516
HpyCH4IV ACGT 2 cut(s) 156, 204
HpyCH4V TGCA 2 cut(s) 493, 533
HpyF10VI GCNNNNNNNGC 2 cut(s) 294, 303
HpyF3I CTNAG 1 cut(s) 366
HpySE526I ACGT 2 cut(s) 156, 204
Hsp92II CATG 3 cut(s) 188, 404, 533
HspAI GCGC 1 cut(s) 446
Kzo9I GATC 4 cut(s) 69, 90, 126, 225
LmnI GCTCC 4 cut(s) 50, 160, 177, 469
Lsp1109I GCAGC 2 cut(s) 192, 262
LweI GCATC 2 cut(s) 244, 544
MaeII ACGT 2 cut(s) 156, 204
MalI GATC 4 cut(s) 71, 92, 128, 227
MboI GATC 4 cut(s) 69, 90, 126, 225
MboII GAAGA 3 cut(s) 19, 85, 238
MhlI GDGCHC 1 cut(s) 424
MlsI TGGCCA 1 cut(s) 399
MluCI AATT 1 cut(s) 561
MluNI TGGCCA 1 cut(s) 399
MlyI GAGTC 1 cut(s) 321
MmeI TCCRAC 2 cut(s) 359, 414
MnlI CCTC 2 cut(s) 140, 478
Mox20I TGGCCA 1 cut(s) 399
MroXI GAANNNNTTC 1 cut(s) 35
MscI TGGCCA 1 cut(s) 399
Msp20I TGGCCA 1 cut(s) 399
MspI CCGG 2 cut(s) 425, 521
MspR9I CCNGG 1 cut(s) 522
MwoI GCNNNNNNNGC 2 cut(s) 294, 303
NciI CCSGG 1 cut(s) 522
NcoI CCATGG 1 cut(s) 400
NdeI CATATG 1 cut(s) 87
NdeII GATC 4 cut(s) 69, 90, 126, 225
NlaIII CATG 3 cut(s) 188, 404, 533
NlaIV GGNNCC 3 cut(s) 268, 423, 465
NsbI TGCGCA 1 cut(s) 447
PdmI GAANNNNTTC 1 cut(s) 35
PkrI GCNGC 2 cut(s) 182, 277
PleI GAGTC 1 cut(s) 321
PpsI GAGTC 1 cut(s) 321
PsiI TTATAA 1 cut(s) 458
PspFI CCCAGC 1 cut(s) 297
PspN4I GGNNCC 3 cut(s) 268, 423, 465
PspPI GGNCC 1 cut(s) 102
PstNI CAGNNNCTG 1 cut(s) 592
SalI GTCGAC 1 cut(s) 324
SatI GCNGC 2 cut(s) 181, 276
Sau3AI GATC 4 cut(s) 69, 90, 126, 225
Sau96I GGNCC 1 cut(s) 102
SchI GAGTC 1 cut(s) 321
ScrFI CCNGG 1 cut(s) 522
SduI GDGCHC 1 cut(s) 424
SfaNI GCATC 2 cut(s) 244, 544
SmlI CTYRAG 1 cut(s) 26
SmoI CTYRAG 1 cut(s) 26
Sse9I AATT 1 cut(s) 561
StyD4I CCNGG 1 cut(s) 520
StyI CCWWGG 2 cut(s) 270, 400
TaaI ACNGT 3 cut(s) 171, 390, 516
TaiI ACGT 2 cut(s) 159, 207
TaqI TCGA 3 cut(s) 21, 146, 325
TasI AATT 1 cut(s) 561
TscAI CASTG 1 cut(s) 174
TseI GCWGC 2 cut(s) 180, 275
TspDTI ATGAA 3 cut(s) 280, 518, 525
TspRI CASTG 1 cut(s) 174
XagI CCTNNNNNAGG 1 cut(s) 446
XmiI GTMKAC 1 cut(s) 325
XmnI GAANNNNTTC 1 cut(s) 35
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.