Rw4G031520

Endoplasmic reticulum membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0002194
Physical Location & Seq
Reverse (-)
58660992 .. 58664609
3618 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G031520.1

Sequence Viewer

Length: 480 bp
ATGGGGAAGACTGGATTGTTCGACCTTGAGAAGCACTTCGCTTTCTATGGAGCTTATCACAGCAATCCGATCAACATAGCGATACATATGATCTTCGTCTGGCCCATCTTCTTCACAGCTCTGTTGCTCCTCTATTTCACGCCTTCCATATTCAACGTGGAGTTTTCACTGTTTGGGAGCCATGTCTTTCTGGCTTTCAACGTCGGCTTCTTGTTGACTTTGATCTATTCCGTGTTTTACTTCTTTCTGGATCATAAAGCTGGTTCCTTGGCTGCTCTGCTTTGTTTTATTTGCTGGGTTGGCAGCAGTTTCCTTGCGAGTCGACTCGGGTTTTCGCTCTCTTGGAAGGTTGTTCTGGTGGCTCAGATAGTGTGTTGGACTGGACAGTTTCTTGGCCATGGGGCCTTTGAGGTATTGAAAACTGTTGGTTTGAACTATCTTTGTGTTGATCTTGTATTAATGCACTCGAAATTTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF962

Protein Analysis

159

Amino Acids

17.99

Weight (kDa)

7.04

Isoelectric Point (pI)

20.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mpo1-like PF06127 8 - 147 1.3e-14 2-hydroxy-palmitic acid dioxygenase Mpo1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 322
AclWI GGATC 1 cut(s) 258
AcoI YGGCCR 1 cut(s) 394
AcsI RAATTY 1 cut(s) 470
AgsI TTSAA 4 cut(s) 154, 199, 418, 433
AluBI AGCT 3 cut(s) 53, 119, 260
AluI AGCT 3 cut(s) 53, 119, 260
AlwI GGATC 1 cut(s) 258
Ama87I CYCGRG 1 cut(s) 326
AoxI GGCC 3 cut(s) 101, 394, 402
ApeKI GCWGC 2 cut(s) 272, 303
ApoI RAATTY 1 cut(s) 470
AseI ATTAAT 1 cut(s) 458
Asp700I GAANNNNTTC 1 cut(s) 35
AspS9I GGNCC 2 cut(s) 102, 402
AvaI CYCGRG 1 cut(s) 326
BalI TGGCCA 1 cut(s) 396
BbsI GAAGAC 1 cut(s) 14
BbvI GCAGC 2 cut(s) 259, 315
BccI CCATC 1 cut(s) 113
BisI GCNGC 2 cut(s) 273, 304
BlsI GCNGC 2 cut(s) 274, 305
BmeT110I CYCGRG 1 cut(s) 326
BmgT120I GGNCC 2 cut(s) 102, 402
BmiI GGNNCC 3 cut(s) 179, 265, 403
BpiI GAAGAC 1 cut(s) 14
BpuEI CTTGAG 1 cut(s) 47
BsaJI CCNNGG 2 cut(s) 267, 397
Bse1I ACTGG 2 cut(s) 16, 385
BseDI CCNNGG 2 cut(s) 267, 397
BseMII CTCAG 1 cut(s) 377
BseNI ACTGG 2 cut(s) 16, 385
BseRI GAGGAG 1 cut(s) 119
BseXI GCAGC 2 cut(s) 259, 315
BseYI CCCAGC 1 cut(s) 294
BshFI GGCC 3 cut(s) 103, 396, 404
BsiHKCI CYCGRG 1 cut(s) 326
BsnI GGCC 3 cut(s) 103, 396, 404
BsoBI CYCGRG 1 cut(s) 326
Bsp143I GATC 5 cut(s) 69, 90, 222, 250, 448
Bsp19I CCATGG 1 cut(s) 397
BspANI GGCC 3 cut(s) 103, 396, 404
BspCNI CTCAG 1 cut(s) 376
BspLI GGNNCC 3 cut(s) 179, 265, 403
BspPI GGATC 1 cut(s) 258
BsrI ACTGG 2 cut(s) 16, 385
BssECI CCNNGG 2 cut(s) 267, 397
BssMI GATC 5 cut(s) 69, 90, 222, 250, 448
BssT1I CCWWGG 2 cut(s) 267, 397
Bst4CI ACNGT 3 cut(s) 171, 387, 424
BstDEI CTNAG 1 cut(s) 363
BstDSI CCRYGG 1 cut(s) 397
BstKTI GATC 5 cut(s) 72, 93, 225, 253, 451
BstMBI GATC 5 cut(s) 69, 90, 222, 250, 448
BstMWI GCNNNNNNNGC 1 cut(s) 300
BstV1I GCAGC 2 cut(s) 259, 315
BstV2I GAAGAC 1 cut(s) 14
BsuRI GGCC 3 cut(s) 103, 396, 404
BtgI CCRYGG 1 cut(s) 397
BtsIMutI CAGTG 1 cut(s) 167
Cfr13I GGNCC 2 cut(s) 102, 402
CviAII CATG 2 cut(s) 182, 398
DdeI CTNAG 1 cut(s) 363
DpnI GATC 5 cut(s) 71, 92, 224, 252, 450
DpnII GATC 5 cut(s) 69, 90, 222, 250, 448
EaeI YGGCCR 1 cut(s) 394
Eco130I CCWWGG 2 cut(s) 267, 397
Eco88I CYCGRG 1 cut(s) 326
EcoO109I RGGNCCY 1 cut(s) 402
EcoT14I CCWWGG 2 cut(s) 267, 397
ErhI CCWWGG 2 cut(s) 267, 397
FaeI CATG 2 cut(s) 185, 401
FaiI YATR 8 cut(s) 48, 77, 87, 89, 149, 183, 255, 399
FatI CATG 2 cut(s) 181, 397
FauNDI CATATG 1 cut(s) 87
FblI GTMKAC 1 cut(s) 322
Fnu4HI GCNGC 2 cut(s) 273, 304
Fsp4HI GCNGC 2 cut(s) 273, 304
GluI GCNGC 2 cut(s) 273, 304
GsaI CCCAGC 1 cut(s) 298
HaeIII GGCC 3 cut(s) 103, 396, 404
Hin1II CATG 2 cut(s) 185, 401
HincII GTYRAC 2 cut(s) 216, 323
HindII GTYRAC 2 cut(s) 216, 323
HinfI GANTC 2 cut(s) 319, 324
Hpy166II GTNNAC 2 cut(s) 216, 323
Hpy188I TCNGA 2 cut(s) 69, 366
Hpy188III TCNNGA 1 cut(s) 248
Hpy8I GTNNAC 2 cut(s) 216, 323
Hpy99I CGWCG 1 cut(s) 206
HpyAV CCTTC 2 cut(s) 153, 340
HpyCH4III ACNGT 3 cut(s) 171, 387, 424
HpyCH4IV ACGT 2 cut(s) 156, 201
HpyCH4V TGCA 1 cut(s) 463
HpyF10VI GCNNNNNNNGC 1 cut(s) 300
HpyF3I CTNAG 1 cut(s) 363
HpySE526I ACGT 2 cut(s) 156, 201
Hsp92II CATG 2 cut(s) 185, 401
Kzo9I GATC 5 cut(s) 69, 90, 222, 250, 448
LmnI GCTCC 3 cut(s) 50, 132, 177
LpnPI CCDG 7 cut(s) 85, 176, 233, 246, 280, 341, 366
Lsp1109I GCAGC 2 cut(s) 259, 315
MaeII ACGT 2 cut(s) 156, 201
MalI GATC 5 cut(s) 71, 92, 224, 252, 450
MboI GATC 5 cut(s) 69, 90, 222, 250, 448
MboII GAAGA 4 cut(s) 19, 85, 100, 103
MlsI TGGCCA 1 cut(s) 396
MluCI AATT 1 cut(s) 470
MluNI TGGCCA 1 cut(s) 396
MlyI GAGTC 2 cut(s) 318, 328
MmeI TCCRAC 1 cut(s) 356
MnlI CCTC 2 cut(s) 140, 403
Mox20I TGGCCA 1 cut(s) 396
MroXI GAANNNNTTC 1 cut(s) 35
MscI TGGCCA 1 cut(s) 396
MseI TTAA 1 cut(s) 458
Msp20I TGGCCA 1 cut(s) 396
MwoI GCNNNNNNNGC 1 cut(s) 300
NcoI CCATGG 1 cut(s) 397
NdeI CATATG 1 cut(s) 87
NdeII GATC 5 cut(s) 69, 90, 222, 250, 448
NlaIII CATG 2 cut(s) 185, 401
NlaIV GGNNCC 3 cut(s) 179, 265, 403
PdmI GAANNNNTTC 1 cut(s) 35
PkrI GCNGC 2 cut(s) 274, 305
PleI GAGTC 2 cut(s) 318, 327
PpsI GAGTC 2 cut(s) 318, 327
PshBI ATTAAT 1 cut(s) 458
PspFI CCCAGC 1 cut(s) 294
PspN4I GGNNCC 3 cut(s) 179, 265, 403
PspPI GGNCC 2 cut(s) 102, 402
SalI GTCGAC 1 cut(s) 321
SaqAI TTAA 1 cut(s) 458
SatI GCNGC 2 cut(s) 273, 304
Sau3AI GATC 5 cut(s) 69, 90, 222, 250, 448
Sau96I GGNCC 2 cut(s) 102, 402
SchI GAGTC 2 cut(s) 318, 328
SetI ASST 8 cut(s) 27, 55, 121, 159, 204, 262, 351, 414
SmlI CTYRAG 1 cut(s) 26
SmoI CTYRAG 1 cut(s) 26
Sse9I AATT 1 cut(s) 470
StyI CCWWGG 2 cut(s) 267, 397
TaaI ACNGT 3 cut(s) 171, 387, 424
TaiI ACGT 2 cut(s) 159, 204
TaqI TCGA 3 cut(s) 21, 322, 467
TasI AATT 1 cut(s) 470
Tru1I TTAA 1 cut(s) 458
Tru9I TTAA 1 cut(s) 458
TscAI CASTG 1 cut(s) 174
TseI GCWGC 2 cut(s) 272, 303
TspGWI ACGGA 1 cut(s) 220
TspRI CASTG 1 cut(s) 174
VspI ATTAAT 1 cut(s) 458
XapI RAATTY 1 cut(s) 470
XcmI CCANNNNNNNNNTGG 1 cut(s) 154
XmiI GTMKAC 1 cut(s) 322
XmnI GAANNNNTTC 1 cut(s) 35
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.