Rroxscaffold_5G00380370

Endoplasmic reticulum membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0002194
Physical Location & Seq
Reverse (-)
60580178 .. 60583802
3625 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00380370.1

Sequence Viewer

Length: 600 bp
ATGGGGAAGACTGGATTGTTCGACCTTGAGAAGCACTTCGCTTTCTATGGAGCTTATCACAGCAATCCGATCAACATAGCGATACATATGATCTTCGTCTGGCCCATCTTCTTCACAGCTCTGTTACTGCTCTATTTCACGCCATCCATATTCAACGTGGAGTTTTCACTGTTTGGGAGCCATGTCTTTCTGGCTTTCAACGTCGGCTTCTTGTTGACTTTGATCTACTCGGTGTTTTACTTCTTTCTGGATCATAAAGCTGGTTCCTTGGCTGCTCTGCTTTGTTTTATTTGCTGGGTTAGCAGTAGTTTCCTTGCGAGTCGACTCGGGTTTTCGCTCTCTTGGAAGGTTGTTCTGGTGGCTCAGATAGTGTGTTGGACTGGACAGTTTATTGGCCATGGGGCCTTTGAGAGACGGGCACCGGCTCTATTGGACAACCTTGCACAAGCATTTATAATGGCTCCTTTCTTTGTTTTGTTAGAGGCTCTGCAAAGCTTCTTTGGCTATGAACCATACCCAGGGTTTCATGCAATAGTTCAGGCAAAAGTTGATGCTGAAATTACTGAATGGAAAGAAACGAAGCAGAGATTGGTCTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF962

Protein Analysis

199

Amino Acids

22.6

Weight (kDa)

6.64

Isoelectric Point (pI)

30.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mpo1-like PF06127 8 - 164 3.6e-22 2-hydroxy-palmitic acid dioxygenase Mpo1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 455
AccB1I GGYRCC 1 cut(s) 418
AccI GTMKAC 1 cut(s) 322
AclWI GGATC 1 cut(s) 258
AcoI YGGCCR 1 cut(s) 394
AfiI CCNNNNNNNGG 1 cut(s) 518
AgsI TTSAA 2 cut(s) 154, 199
AjnI CCWGG 1 cut(s) 517
AjuI GAANNNNNNNTTGG 1 cut(s) 572
AluBI AGCT 4 cut(s) 53, 119, 260, 495
AluI AGCT 4 cut(s) 53, 119, 260, 495
Alw26I GTCTC 1 cut(s) 406
AlwI GGATC 1 cut(s) 258
Ama87I CYCGRG 1 cut(s) 326
AoxI GGCC 3 cut(s) 101, 394, 402
ApeKI GCWGC 1 cut(s) 272
Asp700I GAANNNNTTC 1 cut(s) 35
AspS9I GGNCC 2 cut(s) 102, 402
AvaI CYCGRG 1 cut(s) 326
BaeGI GKGCMC 1 cut(s) 421
BalI TGGCCA 1 cut(s) 396
BanI GGYRCC 1 cut(s) 418
BbsI GAAGAC 1 cut(s) 14
BbvI GCAGC 1 cut(s) 259
BccI CCATC 2 cut(s) 113, 151
BciT130I CCWGG 1 cut(s) 519
BcoDI GTCTC 1 cut(s) 406
BisI GCNGC 1 cut(s) 273
BlsI GCNGC 1 cut(s) 274
Bme1390I CCNGG 1 cut(s) 519
BmeT110I CYCGRG 1 cut(s) 326
BmgT120I GGNCC 2 cut(s) 102, 402
BmiI GGNNCC 5 cut(s) 179, 265, 403, 420, 462
BmrFI CCNGG 1 cut(s) 519
BmsI GCATC 1 cut(s) 541
BpiI GAAGAC 1 cut(s) 14
BpuEI CTTGAG 1 cut(s) 47
BsaJI CCNNGG 4 cut(s) 267, 397, 517, 518
Bsc4I CCNNNNNNNGG 1 cut(s) 518
Bse118I RCCGGY 1 cut(s) 421
Bse1I ACTGG 2 cut(s) 16, 385
BseBI CCWGG 1 cut(s) 519
BseDI CCNNGG 4 cut(s) 267, 397, 517, 518
BseGI GGATG 1 cut(s) 143
BseLI CCNNNNNNNGG 1 cut(s) 518
BseMII CTCAG 1 cut(s) 377
BseNI ACTGG 2 cut(s) 16, 385
BseSI GKGCMC 1 cut(s) 421
BseXI GCAGC 1 cut(s) 259
BseYI CCCAGC 1 cut(s) 294
BshFI GGCC 3 cut(s) 103, 396, 404
BshNI GGYRCC 1 cut(s) 418
BsiHKCI CYCGRG 1 cut(s) 326
BsiSI CCGG 1 cut(s) 422
BslI CCNNNNNNNGG 1 cut(s) 518
BsmAI GTCTC 1 cut(s) 406
BsmBI CGTCTC 1 cut(s) 406
BsnI GGCC 3 cut(s) 103, 396, 404
BsoBI CYCGRG 1 cut(s) 326
Bsp1286I GDGCHC 1 cut(s) 421
Bsp143I GATC 4 cut(s) 69, 90, 222, 250
Bsp19I CCATGG 1 cut(s) 397
BspANI GGCC 3 cut(s) 103, 396, 404
BspCNI CTCAG 1 cut(s) 376
BspLI GGNNCC 5 cut(s) 179, 265, 403, 420, 462
BspPI GGATC 1 cut(s) 258
BspT107I GGYRCC 1 cut(s) 418
BsrFI RCCGGY 1 cut(s) 421
BsrI ACTGG 2 cut(s) 16, 385
BssAI RCCGGY 1 cut(s) 421
BssECI CCNNGG 4 cut(s) 267, 397, 517, 518
BssMI GATC 4 cut(s) 69, 90, 222, 250
BssT1I CCWWGG 2 cut(s) 267, 397
Bst2UI CCWGG 1 cut(s) 519
Bst4CI ACNGT 2 cut(s) 171, 387
BstDEI CTNAG 1 cut(s) 363
BstDSI CCRYGG 1 cut(s) 397
BstF5I GGATG 1 cut(s) 143
BstKTI GATC 4 cut(s) 72, 93, 225, 253
BstMAI GTCTC 1 cut(s) 406
BstMBI GATC 4 cut(s) 69, 90, 222, 250
BstMWI GCNNNNNNNGC 2 cut(s) 300, 501
BstNI CCWGG 1 cut(s) 519
BstSCI CCNGG 1 cut(s) 517
BstSLI GKGCMC 1 cut(s) 421
BstV1I GCAGC 1 cut(s) 259
BstV2I GAAGAC 1 cut(s) 14
BsuRI GGCC 3 cut(s) 103, 396, 404
BtgI CCRYGG 1 cut(s) 397
BtsCI GGATG 1 cut(s) 143
BtsIMutI CAGTG 1 cut(s) 167
Cfr10I RCCGGY 1 cut(s) 421
Cfr13I GGNCC 2 cut(s) 102, 402
CviAII CATG 3 cut(s) 182, 398, 527
DdeI CTNAG 1 cut(s) 363
DpnI GATC 4 cut(s) 71, 92, 224, 252
DpnII GATC 4 cut(s) 69, 90, 222, 250
EaeI YGGCCR 1 cut(s) 394
Eco130I CCWWGG 2 cut(s) 267, 397
Eco88I CYCGRG 1 cut(s) 326
EcoO109I RGGNCCY 1 cut(s) 402
EcoRII CCWGG 1 cut(s) 517
EcoT14I CCWWGG 2 cut(s) 267, 397
ErhI CCWWGG 2 cut(s) 267, 397
Esp3I CGTCTC 1 cut(s) 406
FaeI CATG 3 cut(s) 185, 401, 530
FatI CATG 3 cut(s) 181, 397, 526
FauNDI CATATG 1 cut(s) 87
FblI GTMKAC 1 cut(s) 322
Fnu4HI GCNGC 1 cut(s) 273
FokI GGATG 1 cut(s) 130
Fsp4HI GCNGC 1 cut(s) 273
GluI GCNGC 1 cut(s) 273
GsaI CCCAGC 1 cut(s) 298
HaeIII GGCC 3 cut(s) 103, 396, 404
HapII CCGG 1 cut(s) 422
Hin1II CATG 3 cut(s) 185, 401, 530
HincII GTYRAC 2 cut(s) 216, 323
HindII GTYRAC 2 cut(s) 216, 323
HindIII AAGCTT 1 cut(s) 493
HinfI GANTC 2 cut(s) 319, 324
HpaII CCGG 1 cut(s) 422
Hpy166II GTNNAC 2 cut(s) 216, 323
Hpy188I TCNGA 2 cut(s) 69, 366
Hpy188III TCNNGA 1 cut(s) 248
Hpy8I GTNNAC 2 cut(s) 216, 323
Hpy99I CGWCG 1 cut(s) 206
HpyAV CCTTC 1 cut(s) 340
HpyCH4III ACNGT 2 cut(s) 171, 387
HpyCH4IV ACGT 2 cut(s) 156, 201
HpyCH4V TGCA 3 cut(s) 443, 490, 530
HpyF10VI GCNNNNNNNGC 2 cut(s) 300, 501
HpyF3I CTNAG 1 cut(s) 363
HpySE526I ACGT 2 cut(s) 156, 201
Hsp92II CATG 3 cut(s) 185, 401, 530
Kzo9I GATC 4 cut(s) 69, 90, 222, 250
LmnI GCTCC 3 cut(s) 50, 177, 466
Lsp1109I GCAGC 1 cut(s) 259
LweI GCATC 1 cut(s) 541
MaeII ACGT 2 cut(s) 156, 201
MaeIII GTNAC 1 cut(s) 123
MalI GATC 4 cut(s) 71, 92, 224, 252
MboI GATC 4 cut(s) 69, 90, 222, 250
MboII GAAGA 4 cut(s) 19, 85, 100, 103
MhlI GDGCHC 1 cut(s) 421
MlsI TGGCCA 1 cut(s) 396
MluCI AATT 1 cut(s) 558
MluNI TGGCCA 1 cut(s) 396
MlyI GAGTC 2 cut(s) 318, 328
MmeI TCCRAC 1 cut(s) 356
MnlI CCTC 1 cut(s) 475
Mox20I TGGCCA 1 cut(s) 396
MroXI GAANNNNTTC 1 cut(s) 35
MscI TGGCCA 1 cut(s) 396
Msp20I TGGCCA 1 cut(s) 396
MspI CCGG 1 cut(s) 422
MspR9I CCNGG 1 cut(s) 519
MvaI CCWGG 1 cut(s) 519
MwoI GCNNNNNNNGC 2 cut(s) 300, 501
NcoI CCATGG 1 cut(s) 397
NdeI CATATG 1 cut(s) 87
NdeII GATC 4 cut(s) 69, 90, 222, 250
NlaIII CATG 3 cut(s) 185, 401, 530
NlaIV GGNNCC 5 cut(s) 179, 265, 403, 420, 462
PasI CCCWGGG 1 cut(s) 518
PdmI GAANNNNTTC 1 cut(s) 35
PkrI GCNGC 1 cut(s) 274
PleI GAGTC 2 cut(s) 318, 327
PpsI GAGTC 2 cut(s) 318, 327
PsiI TTATAA 1 cut(s) 455
Psp6I CCWGG 1 cut(s) 517
PspFI CCCAGC 1 cut(s) 294
PspGI CCWGG 1 cut(s) 517
PspN4I GGNNCC 5 cut(s) 179, 265, 403, 420, 462
PspPI GGNCC 2 cut(s) 102, 402
SalI GTCGAC 1 cut(s) 321
SatI GCNGC 1 cut(s) 273
Sau3AI GATC 4 cut(s) 69, 90, 222, 250
Sau96I GGNCC 2 cut(s) 102, 402
SchI GAGTC 2 cut(s) 318, 328
ScrFI CCNGG 1 cut(s) 519
SduI GDGCHC 1 cut(s) 421
SetI ASST 9 cut(s) 27, 55, 121, 159, 204, 262, 351, 441, 497
SfaNI GCATC 1 cut(s) 541
SmlI CTYRAG 1 cut(s) 26
SmoI CTYRAG 1 cut(s) 26
Sse9I AATT 1 cut(s) 558
StyD4I CCNGG 1 cut(s) 517
StyI CCWWGG 2 cut(s) 267, 397
TaaI ACNGT 2 cut(s) 171, 387
TaiI ACGT 2 cut(s) 159, 204
TaqI TCGA 2 cut(s) 21, 322
TasI AATT 1 cut(s) 558
TscAI CASTG 1 cut(s) 174
TseI GCWGC 1 cut(s) 272
TspDTI ATGAA 2 cut(s) 515, 522
TspRI CASTG 1 cut(s) 174
XcmI CCANNNNNNNNNTGG 1 cut(s) 154
XmiI GTMKAC 1 cut(s) 322
XmnI GAANNNNTTC 1 cut(s) 35
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.