MD13G1069400.v1.1

Endoplasmic reticulum membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0002194
Physical Location & Seq
Reverse (-)
4875640 .. 4877063
1424 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1069400.v1.1.491

Sequence Viewer

Length: 588 bp
ATGGGGAAGACCGGCCTGTTCGATCTCGAAAAGCACTTTGCTTTCTACGGAGCTTACCACAGCAACCCAGTCAACATAGCGATTCACATGCTCTTCGTCTGGCCAATCCTTTTCACCTCTCTGCTCATCCTCTACTTCACGCCCTCGCTGCTCAGTTTTGCGGTTAACGACGTCGTTATTCCTTTCAACATCGGCTTGCTGTTGACTATAATCTATTCGGTGTTTTACATCTGTTTGGACGCAAAAGCTGGCTCCTTGGGTGCTCTGCTCTGTGTAATTTGCTGGGTTGGTAGTTGTTTCCTTGGGACCCTACTCGGATTTTCCATCTCTTGGAAGGTTGTTCTGGTGACTCAGATAGTGTGTTGGACTGGACAATTTATTGGCCATGGGGCCTTTGAGAAAAGGGCACCAGCTTTGTTGGACAATCTTGCACAGGCCTTTATAATGGCTCCCTTCTTTGTTCTGTTAGAGGCTCTGCAAATGTTCTTCGGCTACGAGCCCTACCCCGGGTTTCAAGCAATTGTTCAGGCAAAGATTGATGCTGAAATCAGTGAATGGCAAGAGAAGAAGAAGAAACTGATATCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF962

Protein Analysis

196

Amino Acids

21.79

Weight (kDa)

6.39

Isoelectric Point (pI)

30.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mpo1-like PF06127 8 - 160 6.1e-22 2-hydroxy-palmitic acid dioxygenase Mpo1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 443
AatII GACGTC 1 cut(s) 174
AccB1I GGYRCC 1 cut(s) 406
AccB7I CCANNNNNTGG 1 cut(s) 330
AciI CCGC 1 cut(s) 161
AcoI YGGCCR 2 cut(s) 101, 382
AcyI GRCGYC 1 cut(s) 171
AfiI CCNNNNNNNGG 3 cut(s) 330, 506, 507
AgsI TTSAA 2 cut(s) 187, 515
AluBI AGCT 3 cut(s) 53, 248, 413
AluI AGCT 3 cut(s) 53, 248, 413
Alw21I GWGCWC 1 cut(s) 265
Ama87I CYCGRG 1 cut(s) 506
AoxI GGCC 5 cut(s) 13, 101, 382, 390, 435
ApeKI GCWGC 1 cut(s) 148
AspS9I GGNCC 2 cut(s) 306, 390
AsuC2I CCSGG 2 cut(s) 507, 508
AsuHPI GGTGA 2 cut(s) 106, 358
AvaI CYCGRG 1 cut(s) 506
AvaII GGWCC 1 cut(s) 306
BaeGI GKGCMC 1 cut(s) 409
BalI TGGCCA 2 cut(s) 103, 384
BanI GGYRCC 1 cut(s) 406
BanII GRGCYC 1 cut(s) 501
BbsI GAAGAC 1 cut(s) 14
Bbv12I GWGCWC 1 cut(s) 265
BbvI GCAGC 1 cut(s) 135
BccI CCATC 1 cut(s) 332
BcgI CGANNNNNNTGC 2 cut(s) 70, 104
BcnI CCSGG 2 cut(s) 507, 508
BisI GCNGC 1 cut(s) 149
BlsI GCNGC 1 cut(s) 150
Bme1390I CCNGG 2 cut(s) 507, 508
Bme18I GGWCC 1 cut(s) 306
BmeT110I CYCGRG 1 cut(s) 506
BmgT120I GGNCC 2 cut(s) 306, 390
BmiI GGNNCC 6 cut(s) 253, 307, 308, 391, 408, 450
BmrFI CCNGG 2 cut(s) 507, 508
BmrI ACTGGG 1 cut(s) 62
BmsI GCATC 1 cut(s) 529
BmuI ACTGGG 1 cut(s) 62
BpiI GAAGAC 1 cut(s) 14
BpuMI CCSGG 2 cut(s) 507, 508
BsaHI GRCGYC 1 cut(s) 171
BsaJI CCNNGG 5 cut(s) 255, 301, 385, 505, 506
Bsc4I CCNNNNNNNGG 3 cut(s) 330, 506, 507
Bse118I RCCGGY 1 cut(s) 11
Bse1I ACTGG 2 cut(s) 68, 373
BseDI CCNNGG 5 cut(s) 255, 301, 385, 505, 506
BseGI GGATG 1 cut(s) 126
BseLI CCNNNNNNNGG 3 cut(s) 330, 506, 507
BseMII CTCAG 2 cut(s) 166, 365
BseNI ACTGG 2 cut(s) 68, 373
BseSI GKGCMC 1 cut(s) 409
BseXI GCAGC 1 cut(s) 135
BseYI CCCAGC 1 cut(s) 282
BshFI GGCC 5 cut(s) 15, 103, 384, 392, 437
BshNI GGYRCC 1 cut(s) 406
BsiHKAI GWGCWC 1 cut(s) 265
BsiHKCI CYCGRG 1 cut(s) 506
BsiSI CCGG 2 cut(s) 12, 507
BslFI GGGAC 1 cut(s) 319
BslI CCNNNNNNNGG 3 cut(s) 330, 506, 507
BsmFI GGGAC 1 cut(s) 319
BsnI GGCC 5 cut(s) 15, 103, 384, 392, 437
BsoBI CYCGRG 1 cut(s) 506
Bsp1286I GDGCHC 3 cut(s) 265, 409, 501
Bsp143I GATC 1 cut(s) 22
Bsp19I CCATGG 1 cut(s) 385
BspACI CCGC 1 cut(s) 161
BspANI GGCC 5 cut(s) 15, 103, 384, 392, 437
BspCNI CTCAG 2 cut(s) 165, 364
BspLI GGNNCC 6 cut(s) 253, 307, 308, 391, 408, 450
BspQI GCTCTTC 1 cut(s) 98
BspT107I GGYRCC 1 cut(s) 406
BsrFI RCCGGY 1 cut(s) 11
BsrI ACTGG 2 cut(s) 68, 373
BssAI RCCGGY 1 cut(s) 11
BssECI CCNNGG 5 cut(s) 255, 301, 385, 505, 506
BssMI GATC 1 cut(s) 22
BssNI GRCGYC 1 cut(s) 171
BssT1I CCWWGG 3 cut(s) 255, 301, 385
Bst6I CTCTTC 1 cut(s) 98
BstACI GRCGYC 1 cut(s) 171
BstC8I GCNNGC 2 cut(s) 197, 250
BstDEI CTNAG 2 cut(s) 152, 351
BstDSI CCRYGG 1 cut(s) 385
BstF5I GGATG 1 cut(s) 126
BstKTI GATC 1 cut(s) 25
BstMBI GATC 1 cut(s) 22
BstMWI GCNNNNNNNGC 1 cut(s) 148
BstNSI RCATGY 1 cut(s) 91
BstSCI CCNGG 2 cut(s) 505, 506
BstSLI GKGCMC 1 cut(s) 409
BstV1I GCAGC 1 cut(s) 135
BstV2I GAAGAC 1 cut(s) 14
BsuRI GGCC 5 cut(s) 15, 103, 384, 392, 437
BtgI CCRYGG 1 cut(s) 385
BtsCI GGATG 1 cut(s) 126
BtsIMutI CAGTG 1 cut(s) 556
Cac8I GCNNGC 2 cut(s) 197, 250
Cfr10I RCCGGY 1 cut(s) 11
Cfr13I GGNCC 2 cut(s) 306, 390
Cfr9I CCCGGG 1 cut(s) 506
CseI GACGC 1 cut(s) 248
CviAII CATG 2 cut(s) 88, 386
DdeI CTNAG 2 cut(s) 152, 351
DpnI GATC 1 cut(s) 24
DpnII GATC 1 cut(s) 22
EaeI YGGCCR 2 cut(s) 101, 382
Eam1104I CTCTTC 1 cut(s) 98
EarI CTCTTC 1 cut(s) 98
Eco130I CCWWGG 3 cut(s) 255, 301, 385
Eco147I AGGCCT 1 cut(s) 437
Eco24I GRGCYC 1 cut(s) 501
Eco32I GATATC 1 cut(s) 582
Eco47I GGWCC 1 cut(s) 306
Eco88I CYCGRG 1 cut(s) 506
EcoO109I RGGNCCY 2 cut(s) 306, 390
EcoRV GATATC 1 cut(s) 582
EcoT14I CCWWGG 3 cut(s) 255, 301, 385
EcoT38I GRGCYC 1 cut(s) 501
ErhI CCWWGG 3 cut(s) 255, 301, 385
FaeI CATG 2 cut(s) 91, 389
FaiI YATR 5 cut(s) 77, 89, 209, 387, 443
FaqI GGGAC 1 cut(s) 319
FatI CATG 2 cut(s) 87, 385
Fnu4HI GCNGC 1 cut(s) 149
FokI GGATG 1 cut(s) 113
FriOI GRGCYC 1 cut(s) 501
Fsp4HI GCNGC 1 cut(s) 149
GluI GCNGC 1 cut(s) 149
GsaI CCCAGC 1 cut(s) 286
HaeIII GGCC 5 cut(s) 15, 103, 384, 392, 437
HapII CCGG 2 cut(s) 12, 507
HgaI GACGC 1 cut(s) 248
Hin1I GRCGYC 1 cut(s) 171
Hin1II CATG 2 cut(s) 91, 389
HincII GTYRAC 3 cut(s) 73, 166, 204
HindII GTYRAC 3 cut(s) 73, 166, 204
HinfI GANTC 2 cut(s) 82, 349
HpaI GTTAAC 1 cut(s) 166
HpaII CCGG 2 cut(s) 12, 507
HphI GGTGA 2 cut(s) 106, 358
Hpy166II GTNNAC 3 cut(s) 73, 166, 204
Hpy188I TCNGA 2 cut(s) 317, 354
Hpy188III TCNNGA 1 cut(s) 26
Hpy8I GTNNAC 3 cut(s) 73, 166, 204
Hpy99I CGWCG 2 cut(s) 173, 176
HpyAV CCTTC 2 cut(s) 328, 463
HpyCH4IV ACGT 1 cut(s) 171
HpyCH4V TGCA 2 cut(s) 431, 478
HpyF10VI GCNNNNNNNGC 1 cut(s) 148
HpyF3I CTNAG 2 cut(s) 152, 351
HpySE526I ACGT 1 cut(s) 171
Hsp92I GRCGYC 1 cut(s) 171
Hsp92II CATG 2 cut(s) 91, 389
KflI GGGWCCC 1 cut(s) 306
KspAI GTTAAC 1 cut(s) 166
Kzo9I GATC 1 cut(s) 22
LguI GCTCTTC 1 cut(s) 98
LmnI GCTCC 3 cut(s) 50, 257, 454
Lsp1109I GCAGC 1 cut(s) 135
LweI GCATC 1 cut(s) 529
MaeII ACGT 1 cut(s) 171
MaeIII GTNAC 1 cut(s) 346
MalI GATC 1 cut(s) 24
MboI GATC 1 cut(s) 22
MboII GAAGA 6 cut(s) 19, 85, 478, 577, 580, 583
MfeI CAATTG 1 cut(s) 519
MhlI GDGCHC 3 cut(s) 265, 409, 501
MlsI TGGCCA 2 cut(s) 103, 384
MluCI AATT 3 cut(s) 276, 374, 519
MluNI TGGCCA 2 cut(s) 103, 384
MlyI GAGTC 1 cut(s) 343
MmeI TCCRAC 2 cut(s) 344, 399
MnlI CCTC 4 cut(s) 127, 140, 154, 463
Mox20I TGGCCA 2 cut(s) 103, 384
MscI TGGCCA 2 cut(s) 103, 384
MseI TTAA 1 cut(s) 165
Msp20I TGGCCA 2 cut(s) 103, 384
MspI CCGG 2 cut(s) 12, 507
MspR9I CCNGG 2 cut(s) 507, 508
MunI CAATTG 1 cut(s) 519
MwoI GCNNNNNNNGC 1 cut(s) 148
NciI CCSGG 2 cut(s) 507, 508
NcoI CCATGG 1 cut(s) 385
NdeII GATC 1 cut(s) 22
NlaIII CATG 2 cut(s) 91, 389
NlaIV GGNNCC 6 cut(s) 253, 307, 308, 391, 408, 450
NmuCI GTSAC 1 cut(s) 346
NspI RCATGY 1 cut(s) 91
PceI AGGCCT 1 cut(s) 437
PciSI GCTCTTC 1 cut(s) 98
PfeI GAWTC 1 cut(s) 82
PflMI CCANNNNNTGG 1 cut(s) 330
PkrI GCNGC 1 cut(s) 150
PleI GAGTC 1 cut(s) 343
PpsI GAGTC 1 cut(s) 343
PpuMI RGGWCCY 1 cut(s) 306
PsiI TTATAA 1 cut(s) 443
Psp5II RGGWCCY 1 cut(s) 306
PspFI CCCAGC 1 cut(s) 282
PspN4I GGNNCC 6 cut(s) 253, 307, 308, 391, 408, 450
PspPI GGNCC 2 cut(s) 306, 390
PspPPI RGGWCCY 1 cut(s) 306
SapI GCTCTTC 1 cut(s) 98
SaqAI TTAA 1 cut(s) 165
SatI GCNGC 1 cut(s) 149
Sau3AI GATC 1 cut(s) 22
Sau96I GGNCC 2 cut(s) 306, 390
SchI GAGTC 1 cut(s) 343
ScrFI CCNGG 2 cut(s) 507, 508
SduI GDGCHC 3 cut(s) 265, 409, 501
SetI ASST 6 cut(s) 55, 119, 174, 250, 339, 415
SfaNI GCATC 1 cut(s) 529
SinI GGWCC 1 cut(s) 306
SmaI CCCGGG 1 cut(s) 508
Sse9I AATT 3 cut(s) 276, 374, 519
SseBI AGGCCT 1 cut(s) 437
SsiI CCGC 1 cut(s) 161
StuI AGGCCT 1 cut(s) 437
StyD4I CCNGG 2 cut(s) 505, 506
StyI CCWWGG 3 cut(s) 255, 301, 385
TaiI ACGT 1 cut(s) 174
TaqI TCGA 2 cut(s) 21, 27
TasI AATT 3 cut(s) 276, 374, 519
TfiI GAWTC 1 cut(s) 82
Tru1I TTAA 1 cut(s) 165
Tru9I TTAA 1 cut(s) 165
TscAI CASTG 1 cut(s) 556
TseFI GTSAC 1 cut(s) 346
TseI GCWGC 1 cut(s) 148
Tsp45I GTSAC 1 cut(s) 346
TspGWI ACGGA 1 cut(s) 63
TspMI CCCGGG 1 cut(s) 506
TspRI CASTG 1 cut(s) 556
Van91I CCANNNNNTGG 1 cut(s) 330
VpaK11BI GGWCC 1 cut(s) 306
XceI RCATGY 1 cut(s) 91
XmaI CCCGGG 1 cut(s) 506
ZraI GACGTC 1 cut(s) 172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.