Rh4CG396400

Endoplasmic reticulum membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0002194
Physical Location & Seq
Reverse (-)
64606838 .. 64608806
1969 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG396400.1

Sequence Viewer

Length: 534 bp
ATGGGGAAGACTGGATTGTTCGACCTTGAGAAGCACTTCGCTTTCTATGGAGCTTATCACAGCAATCCGATCAACATAGCGATACATATGATCTTCGTCTGGCCCATCTTCTTCACAGCTCTGTTACTCCTCTATTTCACGCCATCCATATTCAACGTGGAGTTTTCACTGTTTGGGAGCCATGTCTTTCTGGCTTTCAACGTCGGCTTCTTGTTGACTTTGATCTATTCCGTGTTTTACTTCTTTCTGGATCATAAAGCTGGTTCCTTGGCTGCTCTGCTTTGTTTTATTTGCTGGGTTGGCAGTAGTTTCCTTGCGAGTCGACTCGGGTTTTCGCTCTCTTGGAAGGTTGTTCTGGTGGCTCAGATAGTGTGTTGGATTGGACAGTTCCTTGGCCATGGGGCCTTTGAGGTATTGAAAACTGTTGGTTTGAATTATCTTTGTGTTGATCTTGTATTAATGCACTTGAAATTTGATTCAATATGTAGAGACGGGCACCGGCTCTATTGGACAACCTTGCACAAGCATTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF962

Protein Analysis

177

Amino Acids

20.25

Weight (kDa)

8.3

Isoelectric Point (pI)

21.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mpo1-like PF06127 8 - 151 7.8e-15 2-hydroxy-palmitic acid dioxygenase Mpo1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 532
AccB1I GGYRCC 1 cut(s) 495
AccI GTMKAC 1 cut(s) 322
AclWI GGATC 1 cut(s) 258
AcoI YGGCCR 1 cut(s) 394
AcsI RAATTY 1 cut(s) 470
AgsI TTSAA 6 cut(s) 154, 199, 418, 433, 469, 480
AluBI AGCT 3 cut(s) 53, 119, 260
AluI AGCT 3 cut(s) 53, 119, 260
Alw26I GTCTC 1 cut(s) 483
AlwI GGATC 1 cut(s) 258
Ama87I CYCGRG 1 cut(s) 326
AoxI GGCC 3 cut(s) 101, 394, 402
ApeKI GCWGC 1 cut(s) 272
ApoI RAATTY 1 cut(s) 470
AseI ATTAAT 1 cut(s) 458
Asp700I GAANNNNTTC 1 cut(s) 35
AspS9I GGNCC 2 cut(s) 102, 402
AvaI CYCGRG 1 cut(s) 326
BaeGI GKGCMC 1 cut(s) 498
BalI TGGCCA 1 cut(s) 396
BanI GGYRCC 1 cut(s) 495
BbsI GAAGAC 1 cut(s) 14
BbvI GCAGC 1 cut(s) 259
BccI CCATC 2 cut(s) 113, 151
BcoDI GTCTC 1 cut(s) 483
BisI GCNGC 1 cut(s) 273
BlsI GCNGC 1 cut(s) 274
BmeT110I CYCGRG 1 cut(s) 326
BmgT120I GGNCC 2 cut(s) 102, 402
BmiI GGNNCC 4 cut(s) 179, 265, 403, 497
BpiI GAAGAC 1 cut(s) 14
BpuEI CTTGAG 1 cut(s) 47
BsaJI CCNNGG 3 cut(s) 267, 391, 397
Bse118I RCCGGY 1 cut(s) 498
Bse1I ACTGG 1 cut(s) 16
BseDI CCNNGG 3 cut(s) 267, 391, 397
BseGI GGATG 1 cut(s) 143
BseMII CTCAG 1 cut(s) 377
BseNI ACTGG 1 cut(s) 16
BseRI GAGGAG 1 cut(s) 119
BseSI GKGCMC 1 cut(s) 498
BseXI GCAGC 1 cut(s) 259
BseYI CCCAGC 1 cut(s) 294
BshFI GGCC 3 cut(s) 103, 396, 404
BshNI GGYRCC 1 cut(s) 495
BsiHKCI CYCGRG 1 cut(s) 326
BsiSI CCGG 1 cut(s) 499
BsmAI GTCTC 1 cut(s) 483
BsmBI CGTCTC 1 cut(s) 483
BsnI GGCC 3 cut(s) 103, 396, 404
BsoBI CYCGRG 1 cut(s) 326
Bsp1286I GDGCHC 1 cut(s) 498
Bsp143I GATC 5 cut(s) 69, 90, 222, 250, 448
Bsp19I CCATGG 1 cut(s) 397
BspANI GGCC 3 cut(s) 103, 396, 404
BspCNI CTCAG 1 cut(s) 376
BspLI GGNNCC 4 cut(s) 179, 265, 403, 497
BspPI GGATC 1 cut(s) 258
BspT107I GGYRCC 1 cut(s) 495
BsrFI RCCGGY 1 cut(s) 498
BsrI ACTGG 1 cut(s) 16
BssAI RCCGGY 1 cut(s) 498
BssECI CCNNGG 3 cut(s) 267, 391, 397
BssMI GATC 5 cut(s) 69, 90, 222, 250, 448
BssT1I CCWWGG 3 cut(s) 267, 391, 397
Bst4CI ACNGT 3 cut(s) 171, 387, 424
BstDEI CTNAG 1 cut(s) 363
BstDSI CCRYGG 1 cut(s) 397
BstF5I GGATG 1 cut(s) 143
BstKTI GATC 5 cut(s) 72, 93, 225, 253, 451
BstMAI GTCTC 1 cut(s) 483
BstMBI GATC 5 cut(s) 69, 90, 222, 250, 448
BstMWI GCNNNNNNNGC 1 cut(s) 300
BstSLI GKGCMC 1 cut(s) 498
BstV1I GCAGC 1 cut(s) 259
BstV2I GAAGAC 1 cut(s) 14
BsuRI GGCC 3 cut(s) 103, 396, 404
BtgI CCRYGG 1 cut(s) 397
BtsCI GGATG 1 cut(s) 143
BtsIMutI CAGTG 1 cut(s) 167
Cfr10I RCCGGY 1 cut(s) 498
Cfr13I GGNCC 2 cut(s) 102, 402
CviAII CATG 2 cut(s) 182, 398
DdeI CTNAG 1 cut(s) 363
DpnI GATC 5 cut(s) 71, 92, 224, 252, 450
DpnII GATC 5 cut(s) 69, 90, 222, 250, 448
EaeI YGGCCR 1 cut(s) 394
Eco130I CCWWGG 3 cut(s) 267, 391, 397
Eco88I CYCGRG 1 cut(s) 326
EcoO109I RGGNCCY 1 cut(s) 402
EcoT14I CCWWGG 3 cut(s) 267, 391, 397
ErhI CCWWGG 3 cut(s) 267, 391, 397
Esp3I CGTCTC 1 cut(s) 483
FaeI CATG 2 cut(s) 185, 401
FatI CATG 2 cut(s) 181, 397
FauNDI CATATG 1 cut(s) 87
FblI GTMKAC 1 cut(s) 322
Fnu4HI GCNGC 1 cut(s) 273
FokI GGATG 1 cut(s) 130
Fsp4HI GCNGC 1 cut(s) 273
GluI GCNGC 1 cut(s) 273
GsaI CCCAGC 1 cut(s) 298
HaeIII GGCC 3 cut(s) 103, 396, 404
HapII CCGG 1 cut(s) 499
Hin1II CATG 2 cut(s) 185, 401
HincII GTYRAC 2 cut(s) 216, 323
HindII GTYRAC 2 cut(s) 216, 323
HinfI GANTC 3 cut(s) 319, 324, 476
HpaII CCGG 1 cut(s) 499
Hpy166II GTNNAC 2 cut(s) 216, 323
Hpy188I TCNGA 2 cut(s) 69, 366
Hpy188III TCNNGA 1 cut(s) 248
Hpy8I GTNNAC 2 cut(s) 216, 323
Hpy99I CGWCG 1 cut(s) 206
HpyAV CCTTC 1 cut(s) 340
HpyCH4III ACNGT 3 cut(s) 171, 387, 424
HpyCH4IV ACGT 2 cut(s) 156, 201
HpyCH4V TGCA 2 cut(s) 463, 520
HpyF10VI GCNNNNNNNGC 1 cut(s) 300
HpyF3I CTNAG 1 cut(s) 363
HpySE526I ACGT 2 cut(s) 156, 201
Hsp92II CATG 2 cut(s) 185, 401
Kzo9I GATC 5 cut(s) 69, 90, 222, 250, 448
LmnI GCTCC 2 cut(s) 50, 177
LpnPI CCDG 7 cut(s) 85, 176, 233, 246, 280, 341, 512
Lsp1109I GCAGC 1 cut(s) 259
MaeII ACGT 2 cut(s) 156, 201
MaeIII GTNAC 1 cut(s) 123
MalI GATC 5 cut(s) 71, 92, 224, 252, 450
MboI GATC 5 cut(s) 69, 90, 222, 250, 448
MboII GAAGA 4 cut(s) 19, 85, 100, 103
MhlI GDGCHC 1 cut(s) 498
MlsI TGGCCA 1 cut(s) 396
MluCI AATT 2 cut(s) 433, 470
MluNI TGGCCA 1 cut(s) 396
MlyI GAGTC 2 cut(s) 318, 328
MmeI TCCRAC 1 cut(s) 356
MnlI CCTC 2 cut(s) 140, 403
Mox20I TGGCCA 1 cut(s) 396
MroXI GAANNNNTTC 1 cut(s) 35
MscI TGGCCA 1 cut(s) 396
MseI TTAA 1 cut(s) 458
Msp20I TGGCCA 1 cut(s) 396
MspI CCGG 1 cut(s) 499
MwoI GCNNNNNNNGC 1 cut(s) 300
NcoI CCATGG 1 cut(s) 397
NdeI CATATG 1 cut(s) 87
NdeII GATC 5 cut(s) 69, 90, 222, 250, 448
NlaIII CATG 2 cut(s) 185, 401
NlaIV GGNNCC 4 cut(s) 179, 265, 403, 497
PdmI GAANNNNTTC 1 cut(s) 35
PfeI GAWTC 1 cut(s) 476
PkrI GCNGC 1 cut(s) 274
PleI GAGTC 2 cut(s) 318, 327
PpsI GAGTC 2 cut(s) 318, 327
PshBI ATTAAT 1 cut(s) 458
PsiI TTATAA 1 cut(s) 532
PspFI CCCAGC 1 cut(s) 294
PspN4I GGNNCC 4 cut(s) 179, 265, 403, 497
PspPI GGNCC 2 cut(s) 102, 402
SalI GTCGAC 1 cut(s) 321
SaqAI TTAA 1 cut(s) 458
SatI GCNGC 1 cut(s) 273
Sau3AI GATC 5 cut(s) 69, 90, 222, 250, 448
Sau96I GGNCC 2 cut(s) 102, 402
SchI GAGTC 2 cut(s) 318, 328
SduI GDGCHC 1 cut(s) 498
SetI ASST 9 cut(s) 27, 55, 121, 159, 204, 262, 351, 414, 518
SmlI CTYRAG 1 cut(s) 26
SmoI CTYRAG 1 cut(s) 26
Sse9I AATT 2 cut(s) 433, 470
StyI CCWWGG 3 cut(s) 267, 391, 397
TaaI ACNGT 3 cut(s) 171, 387, 424
TaiI ACGT 2 cut(s) 159, 204
TaqI TCGA 2 cut(s) 21, 322
TasI AATT 2 cut(s) 433, 470
TfiI GAWTC 1 cut(s) 476
Tru1I TTAA 1 cut(s) 458
Tru9I TTAA 1 cut(s) 458
TscAI CASTG 1 cut(s) 174
TseI GCWGC 1 cut(s) 272
TspGWI ACGGA 1 cut(s) 220
TspRI CASTG 1 cut(s) 174
VspI ATTAAT 1 cut(s) 458
XapI RAATTY 1 cut(s) 470
XcmI CCANNNNNNNNNTGG 1 cut(s) 154
XmiI GTMKAC 1 cut(s) 322
XmnI GAANNNNTTC 1 cut(s) 35
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.