Rroxscaffold_3G00271780

Endoplasmic reticulum membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0002194
Physical Location & Seq
Reverse (-)
64127371 .. 64130956
3586 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00271780.1

Sequence Viewer

Length: 591 bp
ATGGGGGAAGAGGATTCTGGGGTTTATCCTGGGTTTTTGTTTAATTTTGGGTTTGTTTTCACTGTGATCTATGCTTTGTATTATGTTGGATTGGATAAGAAAGCTGGGTCCTTGGCTGCTTTGCTCTGTGTTCTCTGCTGGGTTGGAGCCAGTGTTCTTGCTAGTCGCCTTGGGTTTTCTTTGGCATGGAAGGCCATGTACAATTTGTGGGAGGGTAATGGGAAAATAGTAAAAGATGTGGTTTTGATCTATTTAAGTTGGAAAGCTCTGGACTTTGCTTCGTTTTGCAATTCAAAGATATATGTTTCTTCCAGAAACACTGTTGGTTGTCTTCTTCCAGTTGTGTTGGCAGCTCAGTTGTTCTGTTGGACCGGACAGTTCATAGGCCATGGGGTGTTTGAGAAACGAGCACCAGCTCTTCTGGACAACCTTGTCCAAGCATTTCTAATGGCCCCTTTCTTTGTGTTACTTGAGGTCCTTCAAACGGCCTTTGGTTACGAACCTTATCCAGGGTTTCATGCAAATGTTAAAGAAAAGATAGAGGCTAATATCAAAGAGTGGCAAGAAGAGAAACAAAAGAAACAATCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF962

Protein Analysis

196

Amino Acids

21.92

Weight (kDa)

7.58

Isoelectric Point (pI)

35.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mpo1-like PF06127 52 - 161 2.4e-12 2-hydroxy-palmitic acid dioxygenase Mpo1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 431
AfaI GTAC 1 cut(s) 200
AfiI CCNNNNNNNGG 2 cut(s) 484, 509
AgsI TTSAA 2 cut(s) 294, 482
AjnI CCWGG 2 cut(s) 28, 508
AloI GAACNNNNNNTCC 2 cut(s) 138, 170
AluBI AGCT 4 cut(s) 104, 266, 353, 416
AluI AGCT 4 cut(s) 104, 266, 353, 416
Alw21I GWGCWC 1 cut(s) 412
AoxI GGCC 4 cut(s) 192, 385, 450, 486
ApeKI GCWGC 2 cut(s) 116, 350
AspS9I GGNCC 4 cut(s) 108, 369, 451, 475
AvaII GGWCC 3 cut(s) 108, 369, 475
BarI GAAGNNNNNNTAC 2 cut(s) 182, 214
BbsI GAAGAC 1 cut(s) 323
Bbv12I GWGCWC 1 cut(s) 412
BbvI GCAGC 2 cut(s) 103, 362
BceAI ACGGC 1 cut(s) 501
BciT130I CCWGG 2 cut(s) 30, 510
BfaI CTAG 1 cut(s) 162
BisI GCNGC 2 cut(s) 117, 351
BlsI GCNGC 2 cut(s) 118, 352
Bme1390I CCNGG 2 cut(s) 30, 510
Bme18I GGWCC 3 cut(s) 108, 369, 475
BmgT120I GGNCC 4 cut(s) 108, 369, 451, 475
BmiI GGNNCC 3 cut(s) 109, 148, 453
BmrFI CCNGG 2 cut(s) 30, 510
BpiI GAAGAC 1 cut(s) 323
BpuEI CTTGAG 1 cut(s) 491
BsaJI CCNNGG 5 cut(s) 29, 111, 169, 388, 509
BsaWI WCCGGW 1 cut(s) 371
BsaXI ACNNNNNCTCC 2 cut(s) 138, 168
Bsc4I CCNNNNNNNGG 2 cut(s) 484, 509
Bse1I ACTGG 2 cut(s) 150, 338
BseBI CCWGG 2 cut(s) 30, 510
BseDI CCNNGG 5 cut(s) 29, 111, 169, 388, 509
BseLI CCNNNNNNNGG 2 cut(s) 484, 509
BseMII CTCAG 1 cut(s) 368
BseNI ACTGG 2 cut(s) 150, 338
BseXI GCAGC 2 cut(s) 103, 362
BseYI CCCAGC 2 cut(s) 104, 138
BshFI GGCC 4 cut(s) 194, 387, 452, 488
BsiHKAI GWGCWC 1 cut(s) 412
BsiSI CCGG 1 cut(s) 372
BslI CCNNNNNNNGG 2 cut(s) 484, 509
BsnI GGCC 4 cut(s) 194, 387, 452, 488
Bsp1286I GDGCHC 1 cut(s) 412
Bsp1407I TGTACA 1 cut(s) 198
Bsp143I GATC 2 cut(s) 66, 246
Bsp19I CCATGG 1 cut(s) 388
BspANI GGCC 4 cut(s) 194, 387, 452, 488
BspCNI CTCAG 1 cut(s) 367
BspLI GGNNCC 3 cut(s) 109, 148, 453
BspQI GCTCTTC 1 cut(s) 423
BsrGI TGTACA 1 cut(s) 198
BsrI ACTGG 2 cut(s) 150, 338
BssECI CCNNGG 5 cut(s) 29, 111, 169, 388, 509
BssMI GATC 2 cut(s) 66, 246
BssT1I CCWWGG 3 cut(s) 111, 169, 388
Bst2UI CCWGG 2 cut(s) 30, 510
Bst4CI ACNGT 3 cut(s) 64, 322, 378
Bst6I CTCTTC 3 cut(s) 3, 423, 561
BstAUI TGTACA 1 cut(s) 198
BstDEI CTNAG 2 cut(s) 354, 588
BstDSI CCRYGG 1 cut(s) 388
BstENI CCTNNNNNAGG 1 cut(s) 507
BstKTI GATC 2 cut(s) 69, 249
BstMBI GATC 2 cut(s) 66, 246
BstMWI GCNNNNNNNGC 1 cut(s) 191
BstNI CCWGG 2 cut(s) 30, 510
BstSCI CCNGG 2 cut(s) 28, 508
BstV1I GCAGC 2 cut(s) 103, 362
BstV2I GAAGAC 1 cut(s) 323
BsuRI GGCC 4 cut(s) 194, 387, 452, 488
BtgI CCRYGG 1 cut(s) 388
BtsIMutI CAGTG 3 cut(s) 60, 157, 318
Cfr13I GGNCC 4 cut(s) 108, 369, 451, 475
Csp6I GTAC 1 cut(s) 199
CviAII CATG 4 cut(s) 186, 196, 389, 518
CviQI GTAC 1 cut(s) 199
DdeI CTNAG 2 cut(s) 354, 588
DpnI GATC 2 cut(s) 68, 248
DpnII GATC 2 cut(s) 66, 246
DrdI GACNNNNNNGTC 1 cut(s) 431
DseDI GACNNNNNNGTC 1 cut(s) 431
Eam1104I CTCTTC 3 cut(s) 3, 423, 561
EarI CTCTTC 3 cut(s) 3, 423, 561
Eco130I CCWWGG 3 cut(s) 111, 169, 388
Eco47I GGWCC 3 cut(s) 108, 369, 475
EcoNI CCTNNNNNAGG 1 cut(s) 507
EcoO109I RGGNCCY 2 cut(s) 108, 475
EcoRII CCWGG 2 cut(s) 28, 508
EcoT14I CCWWGG 3 cut(s) 111, 169, 388
ErhI CCWWGG 3 cut(s) 111, 169, 388
FaeI CATG 4 cut(s) 189, 199, 392, 521
FaiI YATR 9 cut(s) 72, 84, 187, 197, 301, 303, 383, 390, 519
FatI CATG 4 cut(s) 185, 195, 388, 517
Fnu4HI GCNGC 2 cut(s) 117, 351
Fsp4HI GCNGC 2 cut(s) 117, 351
FspBI CTAG 1 cut(s) 162
GluI GCNGC 2 cut(s) 117, 351
GsaI CCCAGC 2 cut(s) 108, 142
HaeIII GGCC 4 cut(s) 194, 387, 452, 488
HapII CCGG 1 cut(s) 372
Hin1II CATG 4 cut(s) 189, 199, 392, 521
HinfI GANTC 1 cut(s) 14
HpaII CCGG 1 cut(s) 372
Hpy188III TCNNGA 3 cut(s) 269, 312, 422
HpyAV CCTTC 2 cut(s) 184, 488
HpyCH4III ACNGT 3 cut(s) 64, 322, 378
HpyCH4V TGCA 2 cut(s) 288, 521
HpyF10VI GCNNNNNNNGC 1 cut(s) 191
HpyF3I CTNAG 2 cut(s) 354, 588
Hsp92II CATG 4 cut(s) 189, 199, 392, 521
Kzo9I GATC 2 cut(s) 66, 246
LguI GCTCTTC 1 cut(s) 423
LmnI GCTCC 1 cut(s) 146
Lsp1109I GCAGC 2 cut(s) 103, 362
MaeI CTAG 1 cut(s) 162
MaeIII GTNAC 2 cut(s) 465, 494
MalI GATC 2 cut(s) 68, 248
MboI GATC 2 cut(s) 66, 246
MboII GAAGA 6 cut(s) 20, 300, 323, 326, 410, 578
MhlI GDGCHC 1 cut(s) 412
MluCI AATT 3 cut(s) 43, 202, 289
MmeI TCCRAC 4 cut(s) 67, 124, 239, 347
MnlI CCTC 4 cut(s) 4, 205, 466, 535
MseI TTAA 3 cut(s) 42, 254, 528
MslI CAYNNNNRTG 1 cut(s) 522
MspI CCGG 1 cut(s) 372
MspR9I CCNGG 2 cut(s) 30, 510
MvaI CCWGG 2 cut(s) 30, 510
MwoI GCNNNNNNNGC 1 cut(s) 191
NcoI CCATGG 1 cut(s) 388
NdeII GATC 2 cut(s) 66, 246
NlaIII CATG 4 cut(s) 189, 199, 392, 521
NlaIV GGNNCC 3 cut(s) 109, 148, 453
PciSI GCTCTTC 1 cut(s) 423
PfeI GAWTC 1 cut(s) 14
PkrI GCNGC 2 cut(s) 118, 352
PpuMI RGGWCCY 2 cut(s) 108, 475
Psp5II RGGWCCY 2 cut(s) 108, 475
Psp6I CCWGG 2 cut(s) 28, 508
PspFI CCCAGC 2 cut(s) 104, 138
PspGI CCWGG 2 cut(s) 28, 508
PspN4I GGNNCC 3 cut(s) 109, 148, 453
PspPI GGNCC 4 cut(s) 108, 369, 451, 475
PspPPI RGGWCCY 2 cut(s) 108, 475
RsaI GTAC 1 cut(s) 200
RsaNI GTAC 1 cut(s) 199
RseI CAYNNNNRTG 1 cut(s) 522
SapI GCTCTTC 1 cut(s) 423
SaqAI TTAA 3 cut(s) 42, 254, 528
SatI GCNGC 2 cut(s) 117, 351
Sau3AI GATC 2 cut(s) 66, 246
Sau96I GGNCC 4 cut(s) 108, 369, 451, 475
ScrFI CCNGG 2 cut(s) 30, 510
SduI GDGCHC 1 cut(s) 412
SetI ASST 7 cut(s) 106, 268, 355, 418, 432, 477, 505
SinI GGWCC 3 cut(s) 108, 369, 475
SmiMI CAYNNNNRTG 1 cut(s) 522
SmlI CTYRAG 1 cut(s) 470
SmoI CTYRAG 1 cut(s) 470
Sse9I AATT 3 cut(s) 43, 202, 289
SspMI CTAG 1 cut(s) 162
StyD4I CCNGG 2 cut(s) 28, 508
StyI CCWWGG 3 cut(s) 111, 169, 388
TaaI ACNGT 3 cut(s) 64, 322, 378
TasI AATT 3 cut(s) 43, 202, 289
TatI WGTACW 1 cut(s) 198
TfiI GAWTC 1 cut(s) 14
Tru1I TTAA 3 cut(s) 42, 254, 528
Tru9I TTAA 3 cut(s) 42, 254, 528
TscAI CASTG 3 cut(s) 67, 157, 325
TseI GCWGC 2 cut(s) 116, 350
TspDTI ATGAA 2 cut(s) 370, 506
TspRI CASTG 3 cut(s) 67, 157, 325
VpaK11BI GGWCC 3 cut(s) 108, 369, 475
XagI CCTNNNNNAGG 1 cut(s) 507
XspI CTAG 1 cut(s) 162
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.