Prupe.5G195600_v2.0.a1

Endoplasmic reticulum membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0002194
Physical Location & Seq
Forward (+)
15856970 .. 15858561
1592 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G195600.1

Sequence Viewer

Length: 591 bp
ATGGGGAGGACTGGGTTGTTTGATCTTGAAAGGCACTTCGCCTTCTACGGCGCATACCACAGCAACCCAGTTAACATTTTGATACACACTGTGTTTGTGTGGCCGATATTGTTCACCTCTCTGGTTCTTTTGTACTTCACACCTTCTCTGTACACTTTCCCAGCTGGGCTCAGTCATGGTTTTCTGTTGAATTTTGGGTTTGTTTTCACTGTGATCTATGCCTTGTTTTACGTCTGCTTGGATAAGAAAGCTGGGTCTTTGGCTGCTTTGCTCTGTGTTCTCTGTTGGGTTGGAGCCAGTTTGCTTGCTGCTCGCCTTGGATTTTCTTTGGCATGGAAGGTTGTACTGGCTGCTCAGTTGTTCTGTTGGACTGGACAGTTCATAGGCCATGGGGTGTTTGAGAAACGAGCACCAGCTCTTTTGGACAACCTCGTTCAAGCATTTCTAATGGCGCCTTTCTTCGTGCTGCTTGAGGTTCTTCAAACAGCGTTCGGCTATGAACCTTATCCCGGGTTTCATGCAAGTGTGAAAGAAAAGATAGACGCCAACCTCAAAGAGTGGTGCGAGAAGAAACAGAAGAAAATCTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF962

Protein Analysis

197

Amino Acids

22.03

Weight (kDa)

8.79

Isoelectric Point (pI)

28.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 451
AcoI YGGCCR 1 cut(s) 101
AcsI RAATTY 1 cut(s) 190
AcyI GRCGYC 2 cut(s) 452, 543
AdeI CACNNNGTG 1 cut(s) 91
AfaI GTAC 3 cut(s) 134, 152, 345
AfiI CCNNNNNNNGG 1 cut(s) 509
AgsI TTSAA 4 cut(s) 29, 190, 437, 482
AluBI AGCT 3 cut(s) 164, 251, 416
AluI AGCT 3 cut(s) 164, 251, 416
Alw21I GWGCWC 1 cut(s) 412
Ama87I CYCGRG 1 cut(s) 509
AoxI GGCC 2 cut(s) 101, 385
ApeKI GCWGC 4 cut(s) 263, 308, 350, 466
ApoI RAATTY 1 cut(s) 190
AspLEI GCGC 2 cut(s) 53, 454
AsuC2I CCSGG 2 cut(s) 510, 511
AsuHPI GGTGA 1 cut(s) 106
AvaI CYCGRG 1 cut(s) 509
BanI GGYRCC 1 cut(s) 451
BanII GRGCYC 1 cut(s) 171
Bbv12I GWGCWC 1 cut(s) 412
BbvI GCAGC 4 cut(s) 250, 295, 337, 453
BceAI ACGGC 1 cut(s) 64
BcnI CCSGG 2 cut(s) 510, 511
BfoI RGCGCY 1 cut(s) 455
BisI GCNGC 4 cut(s) 264, 309, 351, 467
BlsI GCNGC 4 cut(s) 265, 310, 352, 468
Bme1390I CCNGG 2 cut(s) 510, 511
BmeT110I CYCGRG 1 cut(s) 509
BmiI GGNNCC 2 cut(s) 295, 453
BmrFI CCNGG 2 cut(s) 510, 511
BmrI ACTGGG 2 cut(s) 21, 62
BmuI ACTGGG 2 cut(s) 21, 62
BpuEI CTTGAG 1 cut(s) 491
BpuMI CCSGG 2 cut(s) 510, 511
BsaHI GRCGYC 2 cut(s) 452, 543
BsaJI CCNNGG 3 cut(s) 316, 388, 509
Bsc4I CCNNNNNNNGG 1 cut(s) 509
Bse1I ACTGG 5 cut(s) 16, 68, 297, 351, 376
BseDI CCNNGG 3 cut(s) 316, 388, 509
BseLI CCNNNNNNNGG 1 cut(s) 509
BseMII CTCAG 2 cut(s) 184, 368
BseNI ACTGG 5 cut(s) 16, 68, 297, 351, 376
BseXI GCAGC 4 cut(s) 250, 295, 337, 453
BseYI CCCAGC 3 cut(s) 160, 164, 251
BshFI GGCC 2 cut(s) 103, 387
BshNI GGYRCC 1 cut(s) 451
BsiHKAI GWGCWC 1 cut(s) 412
BsiHKCI CYCGRG 1 cut(s) 509
BsiSI CCGG 1 cut(s) 510
BslI CCNNNNNNNGG 1 cut(s) 509
BsnI GGCC 2 cut(s) 103, 387
BsoBI CYCGRG 1 cut(s) 509
Bsp1286I GDGCHC 2 cut(s) 171, 412
Bsp1407I TGTACA 1 cut(s) 150
Bsp143I GATC 2 cut(s) 22, 213
Bsp19I CCATGG 1 cut(s) 388
BspANI GGCC 2 cut(s) 103, 387
BspCNI CTCAG 2 cut(s) 183, 367
BspLI GGNNCC 2 cut(s) 295, 453
BspT107I GGYRCC 1 cut(s) 451
BsrGI TGTACA 1 cut(s) 150
BsrI ACTGG 5 cut(s) 16, 68, 297, 351, 376
BssECI CCNNGG 3 cut(s) 316, 388, 509
BssMI GATC 2 cut(s) 22, 213
BssNI GRCGYC 2 cut(s) 452, 543
BssT1I CCWWGG 2 cut(s) 316, 388
Bst4CI ACNGT 3 cut(s) 91, 211, 378
BstACI GRCGYC 2 cut(s) 452, 543
BstAUI TGTACA 1 cut(s) 150
BstC8I GCNNGC 2 cut(s) 306, 313
BstDEI CTNAG 3 cut(s) 170, 354, 588
BstDSI CCRYGG 1 cut(s) 388
BstH2I RGCGCY 1 cut(s) 455
BstHHI GCGC 2 cut(s) 53, 454
BstKTI GATC 2 cut(s) 25, 216
BstMBI GATC 2 cut(s) 22, 213
BstSCI CCNGG 2 cut(s) 508, 509
BstV1I GCAGC 4 cut(s) 250, 295, 337, 453
BsuRI GGCC 2 cut(s) 103, 387
BtgI CCRYGG 1 cut(s) 388
BtsIMutI CAGTG 2 cut(s) 87, 207
Cac8I GCNNGC 2 cut(s) 306, 313
CfoI GCGC 2 cut(s) 53, 454
Cfr9I CCCGGG 1 cut(s) 509
CseI GACGC 1 cut(s) 551
Csp6I GTAC 3 cut(s) 133, 151, 344
CviAII CATG 4 cut(s) 176, 333, 389, 518
CviQI GTAC 3 cut(s) 133, 151, 344
DdeI CTNAG 3 cut(s) 170, 354, 588
DinI GGCGCC 1 cut(s) 453
DpnI GATC 2 cut(s) 24, 215
DpnII GATC 2 cut(s) 22, 213
DraIII CACNNNGTG 1 cut(s) 91
EaeI YGGCCR 1 cut(s) 101
Eco130I CCWWGG 2 cut(s) 316, 388
Eco24I GRGCYC 1 cut(s) 171
Eco88I CYCGRG 1 cut(s) 509
EcoT14I CCWWGG 2 cut(s) 316, 388
EcoT38I GRGCYC 1 cut(s) 171
EgeI GGCGCC 1 cut(s) 453
EheI GGCGCC 1 cut(s) 453
ErhI CCWWGG 2 cut(s) 316, 388
FaeI CATG 4 cut(s) 179, 336, 392, 521
FaiI YATR 8 cut(s) 55, 177, 219, 334, 383, 390, 498, 519
FatI CATG 4 cut(s) 175, 332, 388, 517
Fnu4HI GCNGC 4 cut(s) 264, 309, 351, 467
FriOI GRGCYC 1 cut(s) 171
Fsp4HI GCNGC 4 cut(s) 264, 309, 351, 467
GlaI GCGC 2 cut(s) 52, 453
GluI GCNGC 4 cut(s) 264, 309, 351, 467
GsaI CCCAGC 3 cut(s) 164, 168, 255
HaeII RGCGCY 1 cut(s) 455
HaeIII GGCC 2 cut(s) 103, 387
HapII CCGG 1 cut(s) 510
HgaI GACGC 1 cut(s) 551
HhaI GCGC 2 cut(s) 53, 454
Hin1I GRCGYC 2 cut(s) 452, 543
Hin1II CATG 4 cut(s) 179, 336, 392, 521
Hin6I GCGC 2 cut(s) 51, 452
HinP1I GCGC 2 cut(s) 51, 452
HincII GTYRAC 1 cut(s) 73
HindII GTYRAC 1 cut(s) 73
HpaI GTTAAC 1 cut(s) 73
HpaII CCGG 1 cut(s) 510
HphI GGTGA 1 cut(s) 106
Hpy166II GTNNAC 3 cut(s) 73, 114, 153
Hpy188III TCNNGA 1 cut(s) 26
Hpy8I GTNNAC 3 cut(s) 73, 114, 153
HpyAV CCTTC 3 cut(s) 52, 153, 331
HpyCH4III ACNGT 3 cut(s) 91, 211, 378
HpyCH4IV ACGT 1 cut(s) 231
HpyCH4V TGCA 1 cut(s) 521
HpyF3I CTNAG 3 cut(s) 170, 354, 588
HpySE526I ACGT 1 cut(s) 231
Hsp92I GRCGYC 2 cut(s) 452, 543
Hsp92II CATG 4 cut(s) 179, 336, 392, 521
HspAI GCGC 2 cut(s) 51, 452
KasI GGCGCC 1 cut(s) 451
KspAI GTTAAC 1 cut(s) 73
Kzo9I GATC 2 cut(s) 22, 213
LmnI GCTCC 1 cut(s) 293
Lsp1109I GCAGC 4 cut(s) 250, 295, 337, 453
MaeII ACGT 1 cut(s) 231
MalI GATC 2 cut(s) 24, 215
MboI GATC 2 cut(s) 22, 213
MboII GAAGA 4 cut(s) 451, 470, 580, 589
MhlI GDGCHC 2 cut(s) 171, 412
MluCI AATT 1 cut(s) 190
Mly113I GGCGCC 1 cut(s) 452
MmeI TCCRAC 2 cut(s) 271, 347
MnlI CCTC 4 cut(s) 127, 440, 466, 560
MseI TTAA 1 cut(s) 72
MslI CAYNNNNRTG 1 cut(s) 522
MspA1I CMGCKG 1 cut(s) 164
MspI CCGG 1 cut(s) 510
MspR9I CCNGG 2 cut(s) 510, 511
NarI GGCGCC 1 cut(s) 452
NciI CCSGG 2 cut(s) 510, 511
NcoI CCATGG 1 cut(s) 388
NdeII GATC 2 cut(s) 22, 213
NlaIII CATG 4 cut(s) 179, 336, 392, 521
NlaIV GGNNCC 2 cut(s) 295, 453
PkrI GCNGC 4 cut(s) 265, 310, 352, 468
PluTI GGCGCC 1 cut(s) 455
PspFI CCCAGC 3 cut(s) 160, 164, 251
PspN4I GGNNCC 2 cut(s) 295, 453
PvuII CAGCTG 1 cut(s) 164
RsaI GTAC 3 cut(s) 134, 152, 345
RsaNI GTAC 3 cut(s) 133, 151, 344
RseI CAYNNNNRTG 1 cut(s) 522
SaqAI TTAA 1 cut(s) 72
SatI GCNGC 4 cut(s) 264, 309, 351, 467
Sau3AI GATC 2 cut(s) 22, 213
ScrFI CCNGG 2 cut(s) 510, 511
SduI GDGCHC 2 cut(s) 171, 412
SfoI GGCGCC 1 cut(s) 453
SmaI CCCGGG 1 cut(s) 511
SmiMI CAYNNNNRTG 1 cut(s) 522
SmlI CTYRAG 1 cut(s) 470
SmoI CTYRAG 1 cut(s) 470
Sse9I AATT 1 cut(s) 190
SspDI GGCGCC 1 cut(s) 451
StyD4I CCNGG 2 cut(s) 508, 509
StyI CCWWGG 2 cut(s) 316, 388
TaaI ACNGT 3 cut(s) 91, 211, 378
TaiI ACGT 1 cut(s) 234
TasI AATT 1 cut(s) 190
TatI WGTACW 3 cut(s) 132, 150, 343
Tru1I TTAA 1 cut(s) 72
Tru9I TTAA 1 cut(s) 72
TscAI CASTG 2 cut(s) 94, 214
TseI GCWGC 4 cut(s) 263, 308, 350, 466
TspDTI ATGAA 3 cut(s) 370, 506, 513
TspMI CCCGGG 1 cut(s) 509
TspRI CASTG 2 cut(s) 94, 214
XapI RAATTY 1 cut(s) 190
XmaI CCCGGG 1 cut(s) 509
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.